TimelineQuestionnaire

& physical exam

SerumDust

sampleBlood RNA

Blood DNA Stool Meconiun

&Stool

Mother n = 326 n = 326 n = 200

Newborns n = 326 n = 326** n = 71** n = 90

Child (3-6 months)

n = 286* n = 78 n = 272‡ n = 245 n = 45

Child(12 months)

n = 280 n = 205 n = 9 n = 272 n = 178 n = 57

Child (24 months)

n = 274 n = 169 n = 82 n = 153 n = 51

Measurements & Analyses

Demographics,exposures &

clinical outcomes

* (x2) validation of lifestyle +

follow-up

sIgE, IgA IgG

levels

**from cord blood

Allergens & endotoxin

levels

‡House floors

(n = 232)child’s bed(n = 347)

Gene expression

**from cord blood

Genotyping& re-seq

Parasite analysis & Kato Katz

Molecular analysis of

gutmicroflora