TM
Product Guide for LudgerTag
2-AB
(2-aminobenzamide) Glycan Labeling Kit containing
2-picoline borane
Part of the Ludger-Velocity™ Fast Glycan Analysis Range.
(Ludger Product Code: LT-KAB-VP24)
Ludger Document # LT-KAB-VP24-Guide-v2.0
Ludger Ltd
Culham Science Centre
Oxford OX14 3EB
United Kingdom
Tel: +44 1865 408 554
Fax: +44 870 163 4620
Email: [email protected]
www.ludger.com
Note: The use of 2-picoline borane in labeling reactions is exclusively licensed to
Ludger Ltd
Ludger Document # LT-KAB-VP24-Guide-v2.0
© Ludger Limited, 2013 Page 2
Contents
Page
Contents .......................................................................................................................................... 2
Specifications for LT-KAB-VP24 .................................................................................................... 3
Kit Contents .................................................................................................................................... 4
Additional Reagents and Equipment Required ............................................................................. 4
Time Line for Labeling .................................................................................................................... 4
Labeling Method ............................................................................................................................. 5
LudgerClean™ S or T1 Post-Labeling Sample Cleanup ............................................................... 6
Analysis of LudgerTag™ 2AB-Labeled Glycans ........................................................................... 7
The Reductive Amination Reaction ............................................................................................... 8
Warranties and Liabilities ............................................................................................................... 9
Document Revision Number .......................................................................................................... 9
Appendix 1: Troubleshooting Guide ........................................................................................... 10
SAFETY DATA SHEET .................................................................................................................. 13
SAFETY DATA SHEET .................................................................................................................. 21
SAFETY DATA SHEET .................................................................................................................. 27
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Specifications for LT-KAB-VP24 LT-KAB-VP24 说明书
Application For labeling of free glycans with 2-aminobenzamide acid (2-AB). 应用: 适用于 2-氨基苯甲酰胺(2-AB)标记游离的多糖。
Description The kit contains reagents for the conjugation of dye to the free reducing end of the glycan
by a reductive amination reaction.
描述: 该试剂盒的染料与多糖的游离还原性末端通过还原性氨基化作用反应形成共轭结合。
Dye Properties Mass = 136.15
染料属性 Fluorescence, ex = 250 nm, em = 428 nm.
O
H2N
H2N
Number of Samples 12 separate analytical samples per set of labeling reagents (24 samples in total for the
kit)
样品数: 每组标记试剂有 12 个单独的分析样品(本试剂盒总共可用于 24 个样品)。
Amount of Sample From 25 pmol up to 25 nmol glycans per sample.
样品量: 每个样品分析的多糖为最少为 25 pmol 最多可达 25 nmol。
Suitable Samples Any purified glycans with free reducing termini can be labeled.
适合的样品: 已经纯化的具有游离还原性末端的多糖均能被标记。
Structural Integrity No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.
结构完整性: 唾液酸、海藻糖、硫酸盐或者磷酸盐的损失不可检出(<2 摩尔百分率)
Labeling Selectivity Essentially stoichiometric labeling.
标记选择性: 基本上是化学计量的标记
Storage: Store at room temperature in the dark. Protect from sources of heat, light, and
moisture. The reagents are stable for at least two years from the manufacture date.
储存: 室温避光保存。避免加热、光照和受潮。所有试剂自生产日期起至少 2 年内性质稳
定
Shipping: The product can be shipped at ambient temperature.
运输: 该产品能够常温运输。
Handling: Ensure that any glass, plasticware or solvents used are free of glycosidases and
environmental carbohydrates. Use powder-free gloves for all sample handling
procedures and avoid contamination with environmental carbohydrate.
All steps involving labeling reagents must be performed in a dry environment with dry
glassware and plasticware. Once individual vials of reagents are opened, their contents
should be used immediately and excess then discarded according to local safety rules.
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操作:确保使用的玻璃器皿、塑料器皿或者溶剂均无糖苷酶和环境中的糖类。处理样品时需佩戴无粉手套避免
环境中糖类的污染。
所有涉及标记试剂的步骤必须在干燥的环境中使用干燥的玻璃和塑料器皿。一旦单个的试剂瓶被打开,里面的
成分必须马上使用,多余的试剂应按当地的安全规范进行处理。
Safety: For research use only. Not for human or drug use
Please read the Safety Data Sheets (SDS's) for all chemicals used. All processes
involving labeling reagents should be performed using appropriate personal safety
protection - eyeglasses, chemically resistant gloves (e.g. nitrile), etc. - and where
appropriate in a laboratory fume cupboard.
安全: 请阅读所有使用到的化学物的材料安全数据(SDS's)清单。所有涉及标记试剂的步骤必须
在实验室通风厨进行,使用者需佩戴个人安全保护装置,如护眼镜、实验服和防化学手套
(如腈)。
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Kit Contents 试剂盒组分:
Each kit contains two labelling reaction sets. Each labeling reaction set consists of one vial of each of the
following: 每个试剂盒包括 2 套标记反应。每套标记反应包括以下试剂各一瓶:
Cat. # Item Quantity
LT-2AB-03
LT-PB-01
LT-ACETIC-DMSO-01
2-AB Dye (2-aminobenzamide)
2PB reductant (2-picoline borane)
30% acetic acid in DMSO
7.5 mg
16.5 mg
500 µL
Additional Reagents and Equipment Required
需要的其它试剂和仪器: Milli Q water or similar
分析纯级别的水,如 MilliQ
Heating block, oven, or similar dry heater (a water bath cannot be used) set at 65°C
恒温器,烤箱或者相似的加热干燥器(不能用水浴),反应温度设置在 65°C
Centrifugal evaporator (e.g. Savant, Heto, or similar)
离心蒸发仪(如 Savant、Heto 等商标的仪器)
Reaction vials (e.g. polypropylene microcentrifuge vials)
反应瓶:(如聚丙烯微量离心瓶)
Note: Further reagents are required if doing the optional post-labeling sample cleanup (see Section on
Sample Cleanup)
注意:如果要清洁标记后的样品需要更多的仪器(见样品纯化)
Time Line for Labeling 标记的时间段:
The LudgerTag™ labeling procedure takes 2 hours with just 1 hour for the actual labelling incubation.
LudgerTag™标记过程需要 2 小时,实际的标记孵育需要正好 1 小时。
Procedure Time Elapsed Time (hours)
进程 时间 进程消耗时间
Transfer samples to reaction tube and dry 30 min 0.5
把样品转到反应管并干燥 30 分钟 0.5 小时
Add water to samples 15 min 0.75
在样品中加入水 15 分钟 0.75 小时
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Make up and add labeling reagent 15 min 1
制作并加入标记试剂 15 分钟 1 小时
Incubate samples with reagent 1 hour 2
用试剂孵育样品 1 小时 2 小时
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Labeling Method 标记方法
1 Purify the glycans 纯化多糖
If necessary, remove non-carbohydrate contaminants from the samples (Ludger product LC-EB10-A6).
如果需要的话,可去除样品中的非碳水化合物的污染(Ludger 产品货号 LC-EB10-A6)
2 Transfer sample to reaction vial 把样品转到反应瓶
The kit is designed to label up to 25 nmols of glycans per reaction. With a single pure glycan as little as 5
pmols per reaction can be labeled and detected in subsequent HPLC analysis. Suitable reaction vials
include small polypropylene microcentrifuge tubes and tubes for PCR work.
本试剂盒每个反应可用于标记最多 25nmols。每个反应少至 5 pmols 的单个纯化的多糖均能被标记,并
在随后的高效液相色谱分析中被检测得到。适合的反应瓶包括小号的聚丙烯微量离心管和 PCR 管。
3 Dry the samples and redissolve in 10 µL of water 干燥样品并重溶于 10ul 水
Dry down the samples if the volume of the sample exceeds 10 µL.
If the samples need to be dried down then this should be done using a centrifugal evaporator. If this is
not possible then freeze drying (lyophilization) can be used with caution (in particular, ensure that the
sample dries to a small, compact mass at the very bottom of the vial). Do not subject samples to high
temperatures (>28°C) or extremes of pH as these conditions will result in acid catalysed loss of sialic
acids (high temperatures, low pH) or epimerization of the glycan reducing terminus (at high pH).
Once the samples are dry then redissolve the glycans in 10 µL of water.
如果样品的体积超过10ul则需要进行干燥,样品干燥必须使用离心蒸发仪。如果没有离心蒸发仪,可小心
使用冻干的方法(特别要确保样品在瓶底成为小而紧密的沉淀)。不要把样品暴露在高温(>28°C)或者
极限pH,这些条件可能导致酸催化而损失唾液酸(高温,低pH)或者多糖还原性末端差向异构化(在高
pH)。一旦样品干燥了,即马上把多糖溶在10ul水中。
4 Prepare the labelling reagent 准备标记试剂
Add 150 µL of kit component LT-ACETIC-DMSO-01 (30% acetic acid in DMSO) to a vial of dye (LT-2AB-
03) and mix by pipette action until the dye is dissolved. Sometimes heat (30-60°C) is required to help
dissolve the dye.
Transfer the 150 µL of dissolved dye solution to a vial of reductant (LT-PB-01) and mix by pipette action
until the reductant is dissolved. Sometimes heat (30-60°C) is required to help dissolve the reductant.
加入 150ul 试剂盒组分 LT-ACETIC-DMSO-01 (30%冰乙酸 DMSO)到染料瓶(货号 LT-2AB- 03),用移液
管吸打混匀直到染料溶解。加热(30-60°C)有助于染料溶解。
把上述 150ul 已经溶解的染料溶液转到还原剂瓶(LT-PB-01),用移液管吸打混匀直到染料溶解。加热
(30-60°C)有助于染料溶解。
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5 Add labeling reagent to samples 把标记的试剂加到样品中
Add 10 µL of labeling reagent to each glycan sample, cap the microtube, mix thoroughly, and then gently
tap to ensure the labeling solution is at the bottom of the vial.
加入 10ul 标记的试剂到每个多糖样品,盖好盖子后充分混匀,然后轻柔敲打确保标记的溶液在瓶底。
6 Incubate 孵育
Place the reaction vials in a heating block, sand tray, or dry oven set at 65°C and incubate for 1 hour.
把反应瓶放在加热器上(沙盘或者烤炉)65°C 孵育 1 小时。
The samples must be completely dissolved in the labeling solution for efficient labeling. To encourage
complete dissolution the samples can be vortexed 10 minutes after the start of the 65°C incubation then
the incubation continued.
为了提高标记效率,样品在标记溶液中必须充分溶解。为了鼓励完全溶解样品,样品在 65°C 孵育开始后
可以旋窝混匀 10 分钟,然后继续孵育。
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7 Centrifuge and cool 离心和冷却
After the incubation period remove the samples, centrifuge the microtubes briefly, and then allow them to
cool completely to room temperature.
在孵育完成后移动样品,轻柔离心微型管,然后在室温下完全冷却。
LudgerClean™ S or T1 Post-Labeling Sample Cleanup
LudgerClean™ S 或 T1 标记后样品纯化
Post-labeling sample cleanup (to remove excess dye and other labeling reagents) is necessary for certain
applications - e.g. subsequent analysis by HPLC. Such cleanup can be achieved using LudgerClean™ S
cartridges (Cat # LC-S-Ax, where x denotes the number of cartridges in the kit) or 96 well compatible
LudgerClean™ T1 cartridges (Cat # LC-T1-Ax, where x denotes the number of cartridges in the kit) both using
the standard protocol included with the kits.
标记后样品纯化(为了去除多余的染料和其它标记试剂)对一些应用是必须的。例如,后续的高效液相色谱。这
种纯化能用 LudgerClean™ S 暗盒(货号 LC-S-Ax, x 代表试剂盒中暗盒的编号)或者兼容 96 孔板的
LudgerClean™ T1 暗盒 (货号 LC-T1-Ax, x 代表试剂盒中暗盒的编号)。这两个方法使用该试剂盒中的标准步骤.
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Analysis of LudgerTag™ 2AB-Labeled Glycans
LudgerTag™ 2AB 标记多糖的分析
LudgerTag™ 2-AB labeled glycans may be studied by a number of different analytical methods including
HPLC, gel electrophoresis, and mass spectrometry. LudgerTag™ 2-AB 标记的多糖能够通过很多不同的分析方
法进行分析,包括高效液相色谱、琼脂糖凝胶电泳和质谱。
HPLC Analysis
LudgerTag™ 2-AB labeled glycan mixtures may be separated and analysed by a variety of HPLC (high
pressure liquid chromatography) methods including LudgerSepHPLC:
LudgerTag™ 2-AB 标记的多糖混合物能够通过多种高效液相色谱方法分离和分析,包括 LudgerSep 高效液相
色谱:
Type of Analysis Column
分析类型 色 谱 柱
Separation of charged and neutral glycans LudgerSep™ C
Profile analysis of neutral and charged glycans LudgerSep™ N
Separation of neutral glycans LudgerSep™ R
The LudgerSep™ N column is an especially powerful tool for the purification and analysis of LudgerTag™
labeled oligosaccharides from complex glycan mixtures. Please contact us for advice regarding your particular
application. LudgerSep™ N 色谱柱是从复杂多糖混合物中纯化和分析寡聚糖特别有力的工具。若有特殊的应
用的疑问请联系我们公司。
Enzymatic Analysis
酶分析
High purity, sequencing grade enzymes (e.g. exoglycosidases) suitable for structural analysis of both N- and
O-linked LudgerTag™ labeled glycans are available from a number of companies.
When selecting glycosidases be especially careful to choose those with formulations that are compatible with
your particular application. For example, some enzymes and enzyme buffers have components that interfere
with certain types of analysis. Please call us for guidance in selecting enzymes and reaction conditions for
your work. 很多公司有出售适合 N- 和 O-linked LudgerTag™标记多糖结构分析的高纯度和测序级的酶(如
外切糖苷酶)。当选择糖苷酶时要特别仔细选择那些和你使用的特殊应用相兼容的配方。例如,一些酶和缓冲
液中的成分会干扰某些分析的类型。请联系我们指导选择适合你工作的酶和反应条件。
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Mass Spectrometry and Electrophoresis
质谱分析和凝胶电泳
LudgerTag™ labeled glycans may also be analysed by mass spectrometry, electrophoresis, and various types
of spectroscopy. Please call us for advice on the analysis conditions most suitable for your intended analyses.
LudgerTag™标记多糖可能通过质谱分析、电泳和多种光谱学类型分析。请联系我们提供最适合你想从事工作
的条件。
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NaCNBH3
The Reductive Amination Reaction
还原性氨基化反应
The labeling reaction involves a two step process (see Figure 1): 标记反应包括 2 个操作过程(见图 1):
1. Schiff's base formation. 席夫碱形成
This requires a glycan with a free reducing terminus which is equilibrium between the ring closed (cyclic)
and ring open (acyclic) forms. The primary amino group of the dye performs a nucleophilic attack on the
carbonyl carbon of the acyclic reducing terminal residue to form a partially stable Schiff's base.
这需要具有游离还原性末端多糖,这是闭环和开环形式的平衡。染料的主要氨基基团以亲质子的形式攻击
开环的还原性末端残基的羰基碳,以形成一个特别的席夫碱。
2. Reduction of the Schiff's base. 席夫碱还原反应
The Schiff's base imine group is chemically reduced to give a stable labeled glycan.
席夫碱亚胺基团通过化学还原产生稳定标记的多糖。
H OH
H O
HO
H+ HO
H OH
H OH
HO H H NH
H OH
O
HO H H NH
H O
O
H OH
HO
H OH
H2N
O
H2
N
NH2
NH2
HO H NH H
H O
O
H OH
HO
H OH
O
H2N
O
H N
HO
H NH H
H OH
O
H OH
HO
Imine
H OH
H2N O
N
HO H NH
H H
O
2PB
+ H2O
H OH
HO
H OH
H2N O
H N
HO H NH
H
O
Labelled Glycan
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Figure 1: Labeling of a glycan with 2-aminobenzamide acid (2-AB) by reductive amination.
图 1:用 2-AB 试剂通过还原性的氨基化作用标记多糖。
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Warranties and Liabilities
Ludger warrants that the above product conforms to the attached analytical documents. Should the product fail
for reasons other than through misuse Ludger will, at its option, replace free of charge or refund the purchase
price. This warranty is exclusive and Ludger makes no other warrants, expressed or implied, including any
implied conditions or warranties of merchantability or fitness for any particular purpose.
Ludger shall not be liable for any incidental, consequential or contingent damages.
This product is intended for in vitro research only.
Document Revision Number
Document # LT-KAB-VP24-Guide-v2.0
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Appendix 1: Troubleshooting Guide 附录 1:故障排除指南
The glycan sample to be labeled, whether a purified glycan or a glycan mixture, must contain a free reducing
terminus, be particle and salt-free, and be presented in a volatile solvent system (preferably pure water).
用于标记的多糖样品(已纯化的多糖或者是多糖混合物)必须包含游离的还原性
末端,必须是颗粒状和无盐份存在, 并且应放在挥发溶解系统。
The following may interfere with the labeling reaction and must be removed from the glycan samples prior to
LudgerTag™ labeling: 以下成分可能干扰标记反应,必须在 LudgerTag™标记前去将它们从多糖样品中去除。
Non-volatile solvents
非挥发性溶剂
Non-volatile salts, in particular transition metal ions
非挥发性盐分,特别是转换的金属离子
Detergents
洗涤剂
Dyes and stains such as Coomassie Blue
染料和染色剂,如考马斯亮蓝。
A range of LudgerClean™ kits for cleaning glycan samples prior to LudgerTag™ labeling is available from
Ludger. 一系列可用于 LudgerTag™标记前清洁多糖的 LudgerClean™试剂盒可以在 Ludger 购买。
The LudgerTag™ labeling protocol is an efficient, robust method. If problems do arise they can normally be
corrected without difficulty. The following is a guide to the most likely problems, possible causes, and
solutions. LudgerTag™标记步骤是高效简便的方法。如果有问题出现也很容易被纠正。以下是最有可能出现
的问题、其可能造成的原因和解决方法的指导。
Poor Incorporation of Dye / Low Labeling Yield
染料结合能力差/低标记产量
Water was not added to the glycans prior to adding the labelling reagent
多糖在加标记试剂之前没有加水。
Please ensure that 10 µL of water is added to the glycans during the labelling step. The water can be used to
solubilise the glycans prior to adding the labelling reagent or added after the labelling reagent. Water is
necessary in order to maximise the labelling efficiency.
在多糖标记步骤确保加入 10ul 水。在标记试剂添加前后可以用于溶解多糖。水分对最大提高标记能力很有必要。
The labeling temperature was incorrect.
标记温度不正确
Please ensure that the oven or heating block is equilibrated to the incubation temperature and that the reaction
tube is subjected to this temperature for the entire labeling period.
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请确保烤炉或者加热器恒定在孵育温度,在整个标记过程反应管放在该温度条件下。
The sample was incompletely solubilised. 样品不完全溶解。
The glycans must be completely dissolved in the labeling mixture for maximum labeling efficiency. Please
ensure that the sample is thoroughly mixed with the labeling reagent prior to the incubation and, as a
precaution, carefully mix the samples 15 minutes after the start of the incubation.
多糖必须在标记混合物完全溶解以达到最大的标记效率。请确保在孵育前样品必须充分混匀。作为一个预防措
施,在孵育开始后混合样品 15 分钟。
The sample contained contaminants that interfered with the labeling. 样品中含有干扰标记的污染物。
Please ensure that the glycans are adequately purified before labeling (see protocol step 1 and the
LudgerClean™ Glycan Cleanup Guide).
请确保多糖在标记前已经足够纯化(见步骤 1 和 LudgerClean™多糖纯化指南)
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The labeling solution was inactive.
标记溶液失效。
Please make up the labeling solution immediately before use - the reagents will lose activity within a few hours
of mixing. 在使用前立即制备标记溶液。该试剂在混合后几小时内将失效。
There was less starting glycan than was originally estimated. 多糖的起始量比起初预计的少
The glycans did not contain a free reducing terminus.
多糖没有游离的还原性末端
The 2-AB dye conjugates to the glycan via the aldehyde group of the free reducing terminus. Alditols and
glycans already conjugated via their reducing terminus (e.g. glycopeptides, glycolipids, and previously labeled
glycans) do not contain a free reducing terminus and so cannot conjugate to the dye.
The glycans were lost during the post-labeling cleanup.
Please ensure that the removal of excess labeling reagents is performed as specified in the cleanup protocol
and that the wash reagents are correctly made. 2-AB 染料可通过游离还原性末端乙醛基团跟多糖共轭。
糖醇被多糖已经通过它们的还原性末端(如糖肽、糖脂和先前已经标记的多糖)形成共轭,因为没有了游离的
还原性末端,所以不能和染料共轭。
The Labeled Samples Contain Fluorescent Non-Carbohydrate Material
标记样品包含荧光非碳水化合物材料
The original glycans contained aldehyde-bearing contaminants. 原始的多糖包含醛酸污染物。
Please ensure that the glycans are adequately purified before labeling (see protocol step 1 and the
LudgerClean Glycan Cleanup Guide).
请确保多糖在标记前已经足够纯化(见步骤 1 和 LudgerClean 多糖纯化指南)
The post-labeling cleanup step did not work correctly. 标记后纯化步骤不正确操作。
Please ensure that the removal of excess labeling reagents is performed as specified in the post-labeling
cleanup protocol and that the wash reagents are correctly made. 请确保多余的标记试剂的去除必须按标记后纯
化步骤的详细说明进行操作,并正确制备纯化试剂。
Selective Loss of Smaller Glycans 小分子多糖的选择性损失。
The cleanup cartridge was not primed correctly. 纯化柱装填不正确。
Please ensure the cartridge is primed correctly and that the cartridge bed is still wet with acetonitrile when the
sample is applied to the disc. 请确保纯化柱正确装填,并确保样品加到操作盘时纯化柱的底床依然用乙腈湿润
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Incorrect wash reagents were used during the post-labeling cleanup. 标记后纯化使用了不正确的试剂。
Please ensure that the wash reagents are correctly prepared. 请确保正确准备纯化试剂。
Selective Loss of Larger Glycans 大分子多糖选择性损失。
The sample was incompletely solubilised. 样品溶解不完全。
The glycans must be completely dissolved in the labeling mixture for maximum labeling efficiency. Larger
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glycans tend to be less soluble in the labeling mixture than small sugars. Please ensure that the sample is
thoroughly mixed with the labeling reagent prior to the incubation and, as a precaution, carefully mix the
samples 15 minutes after the start of the incubation. 多糖在标记混合物中必须充分溶解以达到最高标记效率。
大分子多糖在标记混合物比小分子糖分更容易损失。请确保样品在孵育前和标记试剂充分混合。作为一个预防
措施,样品在孵育开始后可以小心混合 15 分钟。
Desialylation of the Glycans
多糖去唾液酸化作用
The sample was subjected to acidic conditions in aqueous solutions at elevated temperatures
样品处在高温的酸性水性溶液中。
Avoid prolonged periods of exposure of sialylated glycan samples in aqueous solutions to conditions of low pH
and elevated temperatures. Keep the incubation time of the labelling reaction to 1h as desialylation increases
with 2 or 3h incubation times.
避免酸化多糖在低 pH 和高温的水性溶液条件中暴露时间过长。保持标记反应的孵育时间达 1 小时,去唾液酸
反应的孵育时间增加到 2 到 3 小时。
The samples were not cleaned up correctly after labeling
标记后的样品纯化不正确。
Make sure that samples undergo the post-labeling cleanup immediately after the reductive amination reaction
and that the post-labeling drying and cleanup procedure is conducted reasonably quickly.
Labeled samples that have not undergone drying and subsequent cleanup will be prone to acid catalyzed de-
sialylation.
确保样品在还原性氨基化反应后 立即在标记后进行纯化,同时确保标记后干燥和纯化步骤快速合理地操作。
标记样品没有经过干燥和后续的纯化会倾向于酸催化去唾液酸作用。
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SAFETY DATA SHEET
Version: 1.0 Date written: 13th
November 2012
SECTION 1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND OF THE
COMPANY / UNDERTAKING
Product Name Acetic Acid / dimethyl sulfoxide solution
Product Catalogue Name LT-ACETIC-DMSO-01
Company: Ludger Ltd Culham Science Centre Abingdon
Oxford OX14 3EB Telephone: 01865 408554 Emergency Telephone: 01865 408554 Email: [email protected]
SECTION 2. HAZARDS IDENTIFICATION
2.1 Classification of the substance or mixture
Classification according to Regulation (EC) No. 1272/2008 [EU-GHS-CLP]
Flammable liquids (Category 3) Skin corrosion (Category 1A)
2.2 Label elements
Signal Word: Danger
Hazard Statement(s)
H226 Flammable liquid and vapour H314 Causes severe skin burns and eye damage.
Precautionary Statement(s)
P280 Wear proactive gloves/ protective clothing/ eye protection/ face protection.
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact
lenses, if present and safe to do so. Continue rinsing. P310 Immediately call a POISON CENTRE or doctor/ physician.
2.3 Other hazard information:
None
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SECTION 3. COMPOSITION/INFORMATION ON INGREDIENTS
3. 1 Substances
Synonyms: DMSO, methyl sulfoxide, dimethyl sulfoxide Glacial acetic acid
Formula: DMSO: C2H6OS
Acetic Acid: C2H4O2
Molecular Weight: DMSO: 78.13 g/mol Acetic Acid: 60.05 g/mol
Component Concentration
Name Dimethyl Sulfoxide 70%
CAS-No. 67-68-5 EC-No. 200-664-3
Name Acetic Acid 30%
CAS-No. 64-19-7 EC-No. 200-580-7 Index-No. 607-002-00-6
SECTION 4. FIRST AID MEASURES
4.1 Description of first aid measures
General Advice
Consult a physician if exposure causes ill effects and if in any doubt. Show this safety data sheet to the physician/ first responder in attendance.
If Ingested
Do NOT induce vomiting. Rinse mouth well with water. Never give anything by mouth to an unconscious person.
If skin is exposed
Remove all contaminated clothing immediately; wash the area well with plenty of soap and water.
If eyes are exposed
Flush eyes with plenty of water/ eye wash solution for at least 15 minutes, if present and safe to do so, remove contact lenses and continue rinsing.
If inhaled
Move effect person to fresh air. If not breathing give artificial respiration.
4.2 Most important symptoms and effects, both acute and delayed
Nausea, Fatigue and Headache. To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated.
4.3 Indication of immediate medical attention and special treatment needed
No data available.
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SECTION 5. FIRE-FIGHTING MEASURES
5.1 Extinguishing media
Small fires: Use extinguishing media such as “alcohol” foam, dry chemical or carbon dioxide. Large fires: Use extinguishing media such as water, from a far away distance as possible. Use very large quantities of water as mist or spray to flood the fire and the combustible material. Cool all affected containers with large quantities of water.
5.2 Special hazards arising from the substance or mixture
Carbon oxides, Sulphur oxides
5.3 Advice for fire fighters
Wear self contained breathing apparatus fir fire fighting if necessary, to spray cool water on any unopened containers near the fire.
SECTION 6. ACCIDENTAL RELEASE MEASURES
6.1 Personal precautions, protective equipment and emergency procedures
Avoid breathing vapours, gas or mist. Remove all sources of ignition. Beware of vapours accumulating to form explosive concentrations. Vapours can accumulate in low areas.
6.2 Environmental Precautions
Prevent further leakage or spillage if safe to do so, e.g. with spill mats. Do not let the product enter drains.
6.3 Methods and material for containment and cleaning up
Contain the spillage and put the collected material into a suitable container with a secure lid. Wash the area well, do not let run off into the drains, collect as waste.
6.4 Reference to other sections
See section 13 for disposal of waste material(s).
SECTION 7. HANDLING AND STORAGE
7.1 Precautions for safe handling
Avoid inhalation of vapour or mist. Keep away from sources of ignition- No smoking. Take measures to prevent the build up of electrostatic charge.
7.2 Conditions for safe storage, including any incompatibilities
Store in a cool place. Keep container closed in a dry well ventilated place.
7.3 Specific end uses
No data available
SECTION 8. EXPOSURE CONTROLS/PERSONAL PROTECTION
8.1 Control parameters
Components with workplace control parameters.
ACETIC ACID
CAS-No. Value Control Parameters
Update Basis
64-19-7 TWA 10ppm 25mg/m3
1991-07-05 Europe. Commission Directive 91/322/EEC on establishing indicative limit on values.
Remarks Indicative
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DMSO contains no substances with occupational exposure limit values.
8.2 Exposure controls
Appropriate engineering controls
Handle in accordance with good laboratory hygiene and safety practice. Wash hands before breaks and at the end of the day.
Personal Protective Equipment
Eye / face protection
Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166 (EU).
Skin protection
Handle with gloves, which should be inspected before use. Use proper glove removal technique (removal without the outside of the glove touching the skin) to avoid contact with the skin/chemical. Dispose of contaminated gloves as Laboratory waste in accordance with applicable laws and good laboratory practices. Wash and dry hands.
Gloves should be of the standard that will stratify the specifications of EU directive 89/696/EEC and the standard EN 374 derived from it.
Body Protection
The type of protective clothing must be selected according to the amount of substance at the specific workplace being used. Impervious coats or laboratory coats.
Respiratory protection
Use substance in an operation fume hood/ outside venting extraction cupboard. Wear full face respirator if appropriate to use, must be tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU).
SECTION 9. PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on basic physical and chemical properties
Appearance Form: Liquid, clear Colour: Colourless
Odour Strong Odour threshold No data available pH No data available Freezing/Melting Point No data available Initial boiling point and boiling range No data available Flash Point No data available Evaporation rate No data available Flammability No data available Upper/lower flammability or explosive limits No data available Vapour Pressure, Pa at temperature degree C No data available Relative Density No data available Solubility in water and solvents Completely miscible Partition coefficient: n-octanol/water No data available Auto ignition temperature No data available Decomposition temperature No data available Viscosity No data available Explosive properties No data available Oxidising properties No data available
9.2 Other information
No data available
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SECTION 10. STABILITY AND REACTIVITY
10.1 eactivity
No data available
10.2 hemical stability
No data available
10.3 ossibility of hazardous reactions
No data available
10.4 onditions to Avoid
Heat, flames and sparks
10.5 Incompatible materials
Acid chlorides, Phosphorus halides, Strong oxidizing agents and strong reducing agents, soluble carbonates and phosphates, hydroxides, metals, peroxides, permanganates, e.g. potassium permanganate, Amines and Alcohols.
10.6 azardous decomposition products
Other decomposition products – No data available
SECTION 11. TOXICOLOGICAL INFORMATION
11.1 Information on toxicological effects
DMSO
Acute toxicity
LD50 Oral – Rat – 14,500mg/kg LC50 Inhalation – Rat – 4h – 40250ppm LD50 Dermal – Rabbit - > 5,000mg/kg
Acetic Acid
Acute toxicity
LD50 Oral – Rat – 3,310 mg/kg
LC50 Inhalation – Mouse – 1h - 5620ppm Remarks: Sense Organs and Special Senses (Nose, Eyes, Ears and Taste): Eyes: Conjunctive irritation. Eyes: Other. Blood: Other changes.
LD50 Dermal – Rabbit – 1,112 mg/kg
DMSO
Skin corrosion/irritation
Skin – Rabbit – No skin irritation – 4h
Acetic Acid
Skin corrosion/irritation
Skin – Rabbit – Mild skin irritation – 24h
DMSO
Serious eye damage/irritation
Eyes – Rabbit – Mild eye irritation
Acetic Acid
Serious eye damage/irritation
Eyes – Rabbit – Corrosive to eyes.
Respiratory or skin sensitisation
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May cause sensitization by skin contact.
Germ cell mutagenicity
Genotoxicity in vitro – Mouse – lymphocyte Cytogenetic analysis
Genotoxicity in vitro – Mouse – lymphocyte Mutation in mammalian somatic cells
Genotoxicity in vivo – Rat – Intraperitoneal Cytogenetic analysis
Genotoxicity in vivo - Mouse – Intraperitoneal DNA damage
Carcinogenicity
Carcinogenicity – Rat – Oral Tumorigenic: Equivocal tumorigenic agent by RTECS criteria. Skin and Appendages: Others: Tumors.
Carcinogenicity – Mouse – Oral Tumorigic: Equivocal tumorigenic agent by RTECS criteria. Lukaemia skin and appendages: Other: Tumors.
IARC: No component of this product presents at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity
Reproductive toxicity – Rat – Intraperitoneal Effects on fertility: Abortion
Reproductive toxicity – Rat – Intraperitoneal Effects on fertility: Post – implantation mortality (e.g. dead and/or resorbed implants per total number of implants).
Reproductive toxicity – Rat – Subcutaneous Effects on fertility: Post – implantation mortality (e.g. dead and/or resorbed implants per total number of implants). Effects on fertility: Litter size (e.g. # fetuses per litter; measured before birth).
Reproductive toxicity – Mouse – Oral Effects on fertility: Pre-implantation mortality (e.g. reduction in number of implants per female; total number of implants per corpora lutea). Effects on Embryo or fetus: Fetoxicity (except death, e.g. stunted fetus). Specific developmental abnormalities: Musculoskeletal system.
Reproductive toxicity – Mouse – Intraperitoneal Effects on embryo or fetus: Fetoxicity (except death, e.g. stunted fetus). Specific developmental abnormalities: Musculoskeletal system.
STOT-single exposure
No data available
STOT-repeated exposure
No data available
Aspiration hazard.
No data available
Potential Health Hazards
Inhalation Harmful if inhaled. Causes serious respiratory tract irritation.
Ingestion Harmful if swallowed. Causes burns.
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Skin May be harmful if absorbed through skin. Causes skin burns.
Eyes Causes eye irritation/ burns.
Aggravated Medical
Condition Avoid contact with DMSO solutions containing toxic materials or
materials with unknown toxicological properties. Dimethyl sulfoxide is readily absorbed through the skin and may carry such materials into the body.
Signs and symptoms of exposure
Nausea, Fatigue, Headache. To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated.
Additional Information RTECS: PV6210000 RTECS: AF1225000
SECTION 12. ECOLOGICAL INFORMATION
12.1 Toxicity
DMSO Toxicity to Fish LC50-Pimephales promelas (fathead minnow) – 34,000mg/l - 96h
96h Toxicity to daphnia and other
LC50-Oncorhynchus mykiss (rainbow trout) – 34,000mg/l-
Aquatic invertebrates EC50-Daphnia pulex (water fleas) – 27,500mg/l Toxicity to algae EC50-Lepomis macrochirus (bluegill) - >400,000mg/l-96h
Acetic Acid Toxicity to Fish LC50 – Leuciscus idus (Golden Orfe) – 410.00mg/l – 48h
LC50 – Cyprinus carpio (Carp) – 49.00mg/l – 48h LC50 – Pimephales promelas (Fathead minnow) – 79.00 -
88.00mg/l – 96h LC50 – Lepomis macrochirus – 75mg/l – 96h
Toxicity to Daphnia and other aquatic invertebrates. EC50 – Daphnia magna (Water flea) – 65.00mg/l – 48h
12.2 ersistence and degradability
Biodegradability Remarks: Expected to be biodegradable.
12.3 ioaccumulative potential
No data available
12.4. Mobility in soil
No data available
12.5. Results of PBT and vPvB assessment
No data available
12.6. Other adverse effects
Biochemical Oxygen Demand (BOD) - 880mg/g
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SECTION 13. DISPOSAL CONSIDERATIONS
13.1 Waste treatment methods
This combustible material may be burned in a chemical incinerator equipped with an afterburner and scrubber or to be disposed of by a licensed professional waste disposal company.
Contaminated packaging
Dispose of as the unused product.
SECTION 14. TRANSPORT INFORMATION
14.1 UN Number
DMSO Acetic Acid
ADR/RID: - ADR/RID: 2789
IMDG: - IMDG: 2789
IATA: - IATA: 2789
14.2 UN Proper Shipping Name DMSO
Acetic Acid
ADR/RID: IMDG: IATA: ADR/RID: IMDG: IATA:
Not Dangerous Goods Not Dangerous Goods Not Dangerous Goods ACETIC ACID, GLACIAL ACETIC ACID, GLACIAL Acetic Acid, glacial
14.3 Transport hazard class (es) DMSO Acetic Acid
ADR/RID: - ADR/RID: 8 (3)
IMDG: - IMDG: 8 (3)
IATA: - IATA: 8 (3)
14.4 Packing group DMSO Acetic Acid
ADR/RID: - ADR/RID: II
IMDG: - IMDG: II
IATA: - IATA: II
14.5 nvironmental hazards
ADR/RID: No IMDG Marine pollutant: No IATA: No
14.6 pecial precautions for user
No data available
SECTION 15. REGULATORY INFORMATION
This safety data sheet complies with the requirements of Regulation (EC) No. 1907/2006
15.1. Safety, health and environmental regulations/legislation specific for the substance or
mixture
No data available
15.2 Chemical Safety Assessment
No data available
Please note that the label elements that used to go in Section 15 are now in Section 2.
SECTION 16. OTHER INFORMATION
The advice offered is derived from the current available information on the hazardous materials in this product and it component(s). Consideration has been made regarding the quantities offered in the pre dispensed container. The advice offered is, therefore not all inclusive nor should it be taken as the descriptive of the compound generally.
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SAFETY DATA SHEET
Version: 1.0
October 2013
Date written: 21st
SECTION 1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND OF THE
COMPANY / UNDERTAKING
Product Name 2-AB Dye
Product Catalogue Name LT-2AB-03
CAS-No. 88-68-6
Company: Ludger Ltd Culham Science Centre Abingdon Oxford OX14 3EB
Telephone: 01865 408554 Emergency Telephone: 01865 408554 Email: [email protected]
SECTION 2. HAZARDS IDENTIFICATION
2.1 Classification of the substance or mixture
Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP]
Acute toxicity, Oral (Category 4) Skin irritation (Category 2)
Eye irritation (Category 2) Respiratory sensitization (Category 1)
Specific target organ toxicity – Single exposure (Category 3)
2.2 Label elements
Signal Word: Danger
Hazard Statement(s)
H302 Harmful if swallowed. H315 Causes skin irritation. H319 Causes serious eye irritation. H334 May cause allergy or asthma symptoms or breathing difficulties if
inhaled.
H335 May cause respiratory irritation.
Precautionary Statement(s)
P261 Avoid breathing dust/ fume/gas/mist/vapours/spray.
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P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do so. Continue rinsing.
P342+P311 If experiencing respiratory symptoms: Call a POISON CENTRE or a Doctor/Physician.
2.3 Other hazard information:
May produce an allergic reaction. No supplemental hazard statements.
SECTION 3. COMPOSITION/INFORMATION ON INGREDIENTS
3. 1 Substances
Synonyms: 2-Aminobenzamide Anthranilic Acid Amide Anthranilamide
Formula: C7H8N2O Molecular Weight: 136.15g/mol
Componen t Concentration
Name Anthranilamide --------
CAS-No. 88-68-6 EC-No. 201-851-2
SECTION 4. FIRST AID MEASURES
4.1 Description of first aid measures
General Advice
Consult a physician if exposure causes ill effects and if in any doubt. Show this safety data sheet to the physician/ first responder in attendance.
If Ingested
Never give anything by mouth to an unconscious person. Rinse mouth well with water.
If skin is exposed
Wash area well with soap and water.
If eyes are exposed
Rinse well with plenty of water for at 15 minutes.
If inhaled
Move the person into fresh air. If not breathing give artificial respiration.
4.2 Most important symptoms and effects, both acute and delayed
To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated so cannot be reported.
4.3 Indication of immediate medical attention and special treatment needed
No Data available
SECTION 5. FIRE-FIGHTING MEASURES
5.1 Extinguishing media
Use a water spray, alcohol resistant foam, dry chemical or carbon dioxide extinguisher.
5.2 Special hazards arising from the substance or mixture
Carbon oxides, nitrogen oxides (NOx)
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5.3 Advice for firefighters
Fire fighters to wear self contained breathing apparatus if necessary.
SECTION 6. ACCIDENTAL RELEASE MEASURES
6.1 Personal precautions, protective equipment and emergency procedures
Use personal protective equipment. Avoid dust formation. Avoid breathing vapours, mist or gas. Ensure adequate ventilation in work areas. Evacuate personnel to safe areas to avoid breathing dust.
6.4 Environmental Precautions
Do not let the product enter the drains.
6.5 Methods and material for containment and cleaning up
Carefully sweep up the spill without creating any dust. Contain the collected material in a sealed suitable container, to await disposal.
6.4 Reference to other sections
Please refer to section 13 for disposal of product and spills.
SECTION 7. HANDLING AND STORAGE
7.1 Precautions for safe handling
Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Provide appropriate exhaust ventilation at places where dust is formed. Normal measures for fire prevention protection.
7.2 Conditions for safe storage, including any incompatibilities
Store in a cool, dark place. Keep the container tightly closed in a dry well ventilated place.
7.3 Specific end uses
No data available
SECTION 8. EXPOSURE CONTROLS/PERSONAL PROTECTION
8.1 Control parameters
Components with workplace control parameters
Contains no substances with occupational exposure limit values.
8.3 Exposure controls
Appropriate engineering controls
Handle in accordance with good laboratory and safety practice. Wash hands before entering the laboratory and at the end of the workday/ when finished handling the material.
Personal Protective Equipment
Eye / face protection
Safety glasses. Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166 (EU).
Skin protection
Handle wearing gloves. Gloves must be inspected before use. Use proper glove removal technique (without the glove touching the skin) to avoid skin contact with the product. Dispose of contaminated gloves as chemical dry waste in accordance with applicable laws and good laboratory practices. Wash and dry the hands. Gloves must satisfy the specifications of EU directive 89/686/EEC and the standard EN 374 derived from it.
Body Protection
Laboratory coat or other types of body covering suitable for use in a laboratory.
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Respiratory protection
When used under an operational fume hood no special protection is required. If required use respirators and components tested and approved under government standards such as NIOSH (US) or CEN (EU). Required level for nuisance exposure P98 (US) or P1 (EU EN 143), higher levels of protection OV/AG/P99 (US) or ABEK-P2 (EU EN 143).
SECTION 9. PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on basic physical and chemical properties
Appearance Form: Crystalline Colour: Beige
Odour No data available Odour threshold No data available pH No data available
Freezing/Melting Point Melting point/range: 111-113C
Melting point/range: 111-113C Initial boiling point and boiling range No data available
Flash Point 198C – closed cup Evaporation rate No data available Flammability No data available Upper/lower flammability or explosive limits No data available Vapour Pressure No data available Relative Density No data available Solubility in water and solvents (mg/l) No data available Partition coefficient: n- Octanol/water No data available Auto ignition temperature No data available Decomposition temperature No data available Viscosity No data available Explosive properties No data available Oxidising properties No data available
9.2 Other information
No data available
SECTION 10. STABILITY AND REACTIVITY
10.1 eactivity
No data available
10.2 hemical stability
No data available
10.3 ossibility of hazardous reactions
No data available
10.4 onditions to Avoid
No data available
10.5 Incompatible materials
Oxidizing agents
10.6 azardous decomposition products
Other decomposition products - No data available
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SECTION 11. TOXICOLOGICAL INFORMATION
11.1 Information on toxicological effects
Acute toxicity
No data available
Skin corrosion/irritation
No data available
Serious eye damage/irritation
No data available
Respiratory or skin sensitisation
No data available May cause sensitisation by inhalation.
Germ cell mutagenicity
No data available
Carcinogenicity
IARC: No components of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity
No data available
STOT-single exposure
Inhalation – May cause respiratory irritation.
STOT-repeated exposure
No data available
Aspiration hazard.
No data available
Potential Health Hazards
Inhalation May be harmful if inhaled. Causes respiratory tract irritation. Ingestion Harmful if swallowed.
Skin May be harmful if absorbed through the skin. Causes skin irritation. Eyes Causes serious eye irritation.
Signs and symptoms of exposure
To the best of our knowledge the chemical, physical and toxicological properties have not been thoroughly investigated.
Additional Information
RTECS: CU8993000
SECTION 12. ECOLOGICAL INFORMATION 12.1 Toxicity
No data available
12.2 ersistence and degradability
No data available
12.3 ioaccumulative potential No data available
12.4. Mobility in soil
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No data available
12.5. Results of PBT and vPvB assessment
No data available
12.6. Other adverse effects
No data available
SECTION 13. DISPOSAL CONSIDERATIONS
13.1 Waste treatment methods
Contact a licensed waste disposal service to collect/dispose of any waste material.
Contaminated packaging
Treat as an unopened/ unused product.
SECTION 14. TRANSPORT INFORMATION
14.1 N Number
ADR/RID: - IMDG: - IATA: -
14.2 N Proper Shipping Name ADR/RID: Not dangerous goods IMDG: Not dangerous goods IATA: Not dangerous goods
14.3 Transport hazard class(es)
ADR/RID: - IMDG: - IATA: -
14.4 Packing group
ADR/RID: -
IMDG: -
IATA: -
14.5 nvironmental hazards
ADR/RID: No IMDG Marine pollutant: No IATA: No
14.6 pecial precautions for user
No data available
SECTION 15. REGULATORY INFORMATION
This safety data sheet complies with the requirements of Regulation (EC) No. 1907/2006
15.1. Safety, health and environmental regulations/legislation specific for the substance or
mixture
No data available
15.2 Chemical Safety Assessment
No data available
Note that the label elements, the Risk and Safety phrases (now Hazard and Precautionary statements) that used to be in Section 15 are now in Section 2.
SECTION 16. OTHER INFORMATION
The advice offered is derived from the current available information on the hazardous materials in this product and it component(s). Consideration has been made regarding the quantities offered in the pre dispensed container. The advice offered is, therefore not all inclusive nor should it be taken as the descriptive of the compound generally.
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SAFETY DATA SHEET
Version: 1.0
October 2013
Date written: 21st
SECTION 1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND OF THE
COMPANY / UNDERTAKING
Product Name 2-Picoline Borane
Product Catalogue Name LT-PB-01
CAS-No: 3999-38-0
Company: Ludger Ltd Culham Science Centre Abingdon
Oxford OX14 3EB Telephone: 01865 408554 Emergency Telephone: 01865 408554 Email: [email protected]
SECTION 2. HAZARDS IDENTIFICATION
2.1 Classification of the substance or mixture
Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP]
Substances, which in contact with water, emit flammable gases (Category 2) Skin irritation (Category 2) Eye irritation (Category 2) Specific target organ toxicity – Single exposure (Category 3)
2.2 Label elements
Signal Word: Danger
Hazard Statement(s)
H261 In contact with water, releases flammable gas. H315 Causes skin irritation. H319 Causes serious eye irritation. H335 May cause respiratory irritation.
Precautionary Statement(s)
P231+P232 Handle under inert gas. Protect from moisture. P261 Avoid breathing dust/ fume/gas/mist/vapours/spray.
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do so. Continue rinsing.
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P422 Store contents under inert gas.
2.3 Other hazard information:
No supplemental hazard statements.
SECTION 3. COMPOSITION/INFORMATION ON INGREDIENTS
3. 1 Substances
Synonyms: 2-picoline borane complex 2-Methylpyridine borane complex
Formula: C6H10NB Molecular Weight: 106.96 g/mol
Componen t Concentration
Name 2-picoline borane complex 100%
CAS-No. 3999-38-0
SECTION 4. FIRST AID MEASURES
4.1 Description of first aid measures
General Advice
Consult a physician if exposure causes ill effects and if in any doubt. Show this safety data sheet to the physician/ first responder in attendance.
If Ingested
Never give anything by mouth to an unconscious person. Rinse mouth well with water.
If skin is exposed
Wash area well with soap and water. Consult a physician.
If eyes are exposed
Rinse well with plenty of water for at 15 minutes and consult a physician.
If inhaled
Move the person into fresh air. If not breathing give artificial respiration. Consult a physician.
4.2 Most important symptoms and effects, both acute and delayed
The most important known symptoms and effects are described in the labelling (see section 2.2)
4.3 Indication of immediate medical attention and special treatment needed
No Data available
SECTION 5. FIRE-FIGHTING MEASURES
5.1 Extinguishing media
Use a dry chemical extinguisher, as it is the only suitable extinguishing media.
5.2 Special hazards arising from the substance or mixture
Carbon oxides, nitrogen oxides (NOx), Borane/ boron oxides.
5.3 Advice for fire fighters
Fire fighters to wear self-contained breathing apparatus if necessary.
SECTION 6. ACCIDENTAL RELEASE MEASURES
6.1 Personal precautions, protective equipment and emergency procedures
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Use personal protective equipment. Avoid dust formation. Avoid breathing vapours, mist or gas. Ensure adequate ventilation in work areas. Evacuate personnel to safe areas to avoid breathing dust.
6.6 Environmental Precautions
Do not let the product enter the drains.
6.7 Methods and material for containment and cleaning up
Carefully sweep up the spill without creating any dust. Contain the collected material in a sealed suitable container, to await disposal. DO NOT USE WATER IN THE CLEANING PROCESS.
6.4 Reference to other sections
Please refer to section 13 for disposal of product and spills.
SECTION 7. HANDLING AND STORAGE
7.1 Precautions for safe handling
Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Provide appropriate exhaust ventilation at places where dust is formed. Keep away from sources of ignition.
7.2 Conditions for safe storage, including any incompatibilities
Store in a cool, dark place. Keep the container tightly closed in a dry well ventilated place.
7.3 Specific end uses
No data available
SECTION 8. EXPOSURE CONTROLS/PERSONAL PROTECTION
8.1 Control parameters
Components with workplace control parameters
Contains no substances with occupational exposure limit values.
8.4 Exposure controls
Appropriate engineering controls
Handle in accordance with good laboratory and safety practice. Wash hands before entering the laboratory and at the end of the workday/ when finished handling the material.
Personal Protective Equipment
Eye / face protection
Safety glasses. Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166 (EU).
Skin protection
Handle wearing gloves. Gloves must be inspected before use. Use proper glove removal technique (without the glove touching the skin) to avoid skin contact with the product. Dispose of contaminated gloves as chemical dry waste in accordance with applicable laws and good laboratory practices. Wash and dry the hands. Gloves must satisfy the specifications of EU directive 89/686/EEC and the standard EN 374 derived from it.
Body Protection
Laboratory coat or other types of body covering suitable for use in a laboratory.
Respiratory protection
When used under an operational fume hood no special protection is required. If required use respirators and components tested and approved under government standards such as NIOSH (US) or CEN (EU). Required level for nuisance exposure P98 (US) or P1 (EU EN 143), higher levels of protection OV/AG/P99 (US) or ABEK-P2 (EU EN 143).
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SECTION 9. PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on basic physical and chemical properties
Appearance Form: Solid Colour: White
Odour No data available Odour threshold No data available pH No data available Freezing/Melting Point Melting point/ range: 44 - 46°C – lit. Initial boiling point and boiling range No data available Flash Point 100°C – closed cup Evaporation rate No data available Flammability No data available Upper/lower flammability or explosive limits No data available Vapour Pressure No data available Relative Density No data available Solubility in water and solvents (mg/l) No data available Partition coefficient: n- Octanol/water No data available Auto ignition temperature No data available
Decomposition temperature No data available Viscosity No data available Explosive properties No data available Oxidising properties No data available
9.2 Other information
No data available
SECTION 10. STABILITY AND REACTIVITY
10.1 eactivity
No data available
10.2 hemical stability
Stable under recommended storage conditions.
10.3 ossibility of hazardous reactions
Reacts violently with water.
10.4 onditions to Avoid
Exposure to moisture.
10.5 Incompatible materials
Strong oxidizing agents
10.6 azardous decomposition products
Other decomposition products - No data available
SECTION 11. TOXICOLOGICAL INFORMATION
11.1 Information on toxicological effects
Acute toxicity
No data available
Skin corrosion/irritation
No data available
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Serious eye damage/irritation
No data available
Respiratory or skin sensitisation
No data available
Germ cell mutagenicity
No data available
Carcinogenicity
IARC: No components of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity
No data available
STOT-single exposure
Inhalation – May cause respiratory irritation.
STOT-repeated exposure
No data available
Aspiration hazard.
No data available
Signs and symptoms of exposure
To the best of our knowledge the chemical, physical and toxicological properties have not been thoroughly investigated.
Additional Information
RTECS: Not available
SECTION 12. ECOLOGICAL INFORMATION
12.1 Toxicity
No data available
12.2 ersistence and degradability
No data available
12.3 ioaccumulative potential
No data available
12.4. Mobility in soil
No data available
12.5. Results of PBT and vPvB assessment
No data available
12.6. Other adverse effects
No data available
SECTION 13. DISPOSAL CONSIDERATIONS
13.1 Waste treatment methods
Contact a licensed waste disposal service to collect/dispose of any waste material. Company should be advised to the nature of the substance, Highly Flammable.
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Contaminated packaging
Treat as an unopened/ unused product.
SECTION 14. TRANSPORT INFORMATION
14.1 N Number
ADR/RID: 2813 IMDG: 2813 IATA: 2813
14.2 N Proper Shipping Name
ADR/RID: WATER-REACTIVE SOLID, N.O.S. (2-Picoline borane complex) IMDG: WATER-REACTIVE SOLID, N.O.S. (2-Picoline borane complex) IATA: Water-reactive solid, n.o.s. (2-Picoline borane complex)
14.3 Transport hazard class(es)
ADR/RID: 4.3 IMDG: 4.3 IATA: 4.3
14.4 Packing group
ADR/RID: II
IMDG: II
IATA: II
14.5 nvironmental hazards
ADR/RID: No IMDG Marine pollutant: No IATA: No
14.6 pecial precautions for user
No data available
SECTION 15. REGULATORY INFORMATION
This safety data sheet complies with the requirements of Regulation (EC) No. 1907/2006
15.1. Safety, health and environmental regulations/legislation specific for the substance or
mixture
No data available
15.2 Chemical Safety Assessment
No data available
SECTION 16. OTHER INFORMATION
The advice offered is derived from the current available information on the hazardous materials in this product and it component(s). Consideration has been made regarding the quantities offered in the pre dispensed container. The advice offered is, therefore not all inclusive nor should it be taken as the descriptive of the compound generally.
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