(For office use only)
Proceedings 58th Annual Maize Workshop
All India Coordinated Research Project on Maize
held at PAU, Ludhiana
April 4-6, 2015
All India Coordinated Research Project on Maize
Indian Institute of Maize Research (Indian Council of Agricultural Research)
Pusa Campus, New Delhi 110 012
www.iimr.res.in
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58th Annual Workshop All India Coordinated Maize Improvement Project
(Indian Council of Agricultural Research)
Date: April 4-6, 2015 Venue: PAU, Ludhiana
Day 1: April 4, 2015 (Saturday)
0800-0900: Registration
Session I: Inaugural Session 0900-1040 Chief Guest : Dr. Gurbachan Singh, Chairman, ASRB, New
Delhi Guest of Honour : Dr. J.S. Sandhu, Dy. Director General (CS),
ICAR Special Guests : Dr A.S. Khehra, Former Vice Chancellor, PAU,
Ludhiana
Dr. S.K. Sharma, Chairman RAC, IIMR, New Delhi
Dr. I.S. Solanki, ADG (FFC), ICAR Presiding Officer : Dr. B.S. Dhillon, Vice-Chancellor, PAU, Ludhiana
Rapporteurs : Drs. P. Kumar, V. Mahajan, A.K. Singh 0900-0905 Welcome : Dr. Balwinder Singh, Director of Research, PAU,
Ludhiana
0905-0910 Lighting of lamp : Chief Guest and other dignitaries 0910-0925 Director’s Review : Dr. O.P. Yadav, Director, IIMR
0925-0940 Remarks by Special Guests
: Dr. I.S. Solanki, ADG (FFC), ICAR Dr. S.K. Sharma, Chairman RAC, IIMR Dr A.S. Khehra, Former Vice Chancellor, PAU,
Ludhiana 0940-0950 Address by Guest of
Honour
: Dr. J.S. Sandhu, DDG (CS), ICAR
0950-1000 Presidential address
: Dr. B.S. Dhillon, Vice-Chancellor, PAU, Ludhiana
1000-1015 Address by Chief Guest
: Dr. Gurbachan Singh, Chairman, ASRB, New Delhi
1015-1035 Felicitation : 1035-1040 Vote of Thanks : Dr. H.S. Dhaliwal, Dean, COA, PAU, Ludhiana
1040-1100 High tea
Session II: Review of work during Kharif 2014 and Rabi 2013-14 1100-1300
Discipline Speaker Breeding : Dr. Bhupender Kumar
Crop Production : Dr. A.K. Singh Entomology : Dr. P. Kumar Pathology/ Nematology : Dr. K.S. Hooda
1300-1400 Lunch Break
Chairman: : Dr. J.S. Sandhu, DDG (CS), ICAR Co-Chairman : Dr. I.S. Solanki, ADG (FFC), ICAR
Rapporteurs: : Drs. J.C. Sekhar, Meena Shekhar, Ashok Kumar, G.K Chikkappa.
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Session III: Panel Discussion - “Doubling maize production of India by 2025 – Opportunities and challenges”
1400 - 1530
Session IV: Variety Identification Committee Meeting 1530-1730
Chairman : Dr. J.S. Sandhu, DDG (CS), ICAR
Co-chairman : Dr. BM Parsanna
Panelists : Dr BS Dhillon, PAU, Ludhiana
Dr SK Vasal, CIMMYT/BISA
Dr BM Prasanna
Dr OP Yadav, IIMR, New Delhi
Dr B Vivek, CIMMYT
Dr G. Nalathambi, TNAU, Coimbatore
Dr ML Jat, CIMMYT
Dr JP Shahi, BHU
Dr H Bhardwaj, Pioneer Oversees Corporation
Dr Dhiraj Pant, Monsanto
Dr Bijender Pal, Bio seed
Dr Usha Singh, RAU
Dr J.S. Chawla, PAU
Dr S.K. Guleria, CSK HPKV
Dr Feroz Hossain, IARI
Dr S Dass
Chairman : Dr. J.S. Sandhu, DDG (CS), ICAR
Co-chairman : Dr. I.S. Solanki, ADG (FFC), ICAR
Member Secretary : Dr. O.P. Yadav, Director, IIMR
Participants : All Members of Variety Identification Committee
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Day 2: April 5, 2015 (Sunday)
Session V: Review of research results of individual AICRP centres for Kharif 2014 and Rabi 2013-14 and formulation of plan of work for Kharif 2015 and
Rabi 2015-16 (Concurrent discipline-wise, centre-wise presentations of significant results and progress report) 0900-1130:
Concurrent
session
Chairman Co-chairman Convener Rapporteur/s
Crop Improvement
Dr. I.S. Solanki, ADG (FFC), ICAR
Dr RK Gumber ADR (CI), PAU,
Ludhiana
Dr. O.P. Yadav
Drs. Vijay, Mahajan, J. Kaul, Bhupender
Kumar, Chikkappa G.K., GK Gill,
Crop Production
Head, Deptt of Agronomy, PAU
Dr. M.L. Jat, Agronomist, CIMMYT-India
Dr. A.K. Singh
Drs. Ashok Kumar, S.L. Jat, Mahesh Kumar
Plant Pathology &
Nematology
Head, Deptt of Plant Pathology,
PAU
Dr VK Kaul Prof.
Nematology PAU, Ludhiana
Dr. K.S. Hooda
Drs. Meena Shekhar, S.S. Sharma,
Chander Mohan
Entomology Head, Deptt. of
Entomology, PAU
- Dr. P.
Kumar
Drs. J.C. Sekhar,
Jawala Jindal
1130-1145 Tea Break
Session VI: Lead Lectures
1145-1300 Chairman : Dr. S.K. Vasal, Eminent Maize Breeder, CIMMYT
Rapporteurs : Drs. P. Yadav, NS Bains, Chikkappa G.K.
Speaker : Enhancing maize genetic gains and breeding
efficiency: status and prospects- Dr. B.M. Prasanna
1300-1400 Lunch Break
Session VII:ICAR-CIMMYT Collaborative Research
1400-1500 Chairman: : Dr. S.K. Vasal, Eminent Maize Breeder,
CIMMYT
Co-chairman : Dr. O.P. Yadav, Director, IIMR, New Delhi
Rapporteurs : Drs. C.M. Parihar, G.K. Chikkappa, G.K. Gill
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1500-1515 Tea Break Session VIII: Breeder Seed Production, FLDs and Training Programmes
Session IX: Germplasm exchange, registration & seed issues 1630-1700
Day 3: April 6, 2015 (Monday)
Session XI: General session
0900-0940 General Discussion and monitoring reports
Chairman: : O.P. Yadav, Director, IIMR
Rapporteurs : J. Kaul, Bhupender Kumar
Session X: Presentations of work plan 2015-16
0940-1100
1100-1130
Tea Break
Results of ICAR-CIMMYT collaborative research (2014)- Dr. C.G. Karjagi, IIMR
Planning ICAR-CIMMYT collaborative research (2015) - Dr. A.R. Sadananda, CIMMYT
1515-1630 Chairman : Dr. I.S. Solanki, ADG (FFC), ICAR
Co–Chairman : Dr. G Nallathambi, Sr. Maize Breeder Rapporteurs: : Drs. Avinash Singode, Tosh Garg Speakers:
: Dr. J. Kaul (Seed)
Dr. Ashok Kumar (FLDs) Dr. Ranveer Singh (ISOPOM)
Chairman
Co-Chair
: Dr SK Sharma
Dr. K.S. Thind
Rapporteurs : Drs. J. Kaul, G.K. Chikkappa, B.S. Gill
Speakers : Dr. J.C. Sekhar, IIMR – Germplasm Exchange by IIMR
Dr. A.R. Sadananda, Germplasm Exchange by CIMMYT Dr. J Kaul, IIMR – Varietal and germplasm
registration and related aspects Dr. Ashok Kumar, NBPGR – Germplasm status in
NBPGR in maize
Chairman: : Dr. I.S. Solanki, ADG (FFC), ICAR Co-Chairman : Dr. K.S. Thind, HOD, PBG, PAU, Ludhiana Rapporteurs:
: Drs. J.C. Sekhar, Meena Shekhar, Ashok Kumar,
Ramesh Kumar, G.K. Chikkappa
Discipline
Breeding
Speaker
: Dr. Bhupender Kumar
Crop Production : Dr. A.K. Singh
Entomology : Dr. P. Kumar
Pathology/
Nematology
: Dr. K.S. Hooda
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Session XII: Plenary Session
1130-1300
Vote of Thanks : Dr. J.S. Chawla, Organizing Secretary
1300-1400 Lunch Break
1400-1700 Field visit
Chairman:
Co-Chair
: Dr. B.S. Dhillon, Vice Chancellor, PAU, Ludhiana
Dr. I.S. Solanki, ADG (FFC), ICAR
Speakers:
Recommendations of VIC Meeting
: Dr. O.P. Yadav, Director, IIMR
Dr. G.K Chikkappa. : Plant Breeding
Dr. Ashok Kumar : Crop Production
Dr. J.C. Sekhar : Entomology
Dr. Meena Shekhar : Pathology/ Nematology
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Session I: Inaugural session
On the occasion, Dr Gurbachan Singh, Chairman, Agricultural Scientists
Recruitment Board, New Delhi was the chief guest while Dr J.S. Sandhu, Deputy Director General (Crop Sciences), Indian Council of Agricultural Research (ICAR)
was the guest of honour. Dr Baldev Singh Dhillon, Vice-Chancellor, PAU presided over the inaugural session. Besides, Dr Amarjit Singh Khera, former Vice-
Chancellor, PAU; Dr S.K. Sharma, Chairman, Research Advisory Committee, Indian Institute of Maize Research (IIMR), New Delhi; and Dr I.S. Solanki, Assistant Director General (Food and Fodder Crops), ICAR were the special
guests. Dr O.P. Yadav, Director, IIMR also shared his views during the workshop.
In his inaugural address, Dr Gurbachan Singh said maize crop is
ecologically suitable for Punjab, Haryana and western Uttar Pradesh. Its cultivation must be promoted among the farmers for it has a great scope in terms of marketing, processing and value addition, he added. ―According to
contemporary farm scenario, many farmers are cultivating small land holdings,‖ he pointed out, while emphasizing on adopting integrated farming approach.
With declining profits and increasing cultivation costs, maize can play a vital role in sustaining productivity, he said. Stating, ―We need to produce more from less area and less water,‖ Dr Singh said water share, in the next 20 years, may drop
from 80 per cent at present to 72 per cent. The power of new sciences such as nanotechnology and biotechnology must be used in various crops, he advised.
Besides, he urged the maize experts to lay stress on density management in maize. In his address, he stressed that yield gap at village, district, state and national level must be analyzed critically followed by an out-of-box thinking to
further augment maize productivity of country. He also advocated to adopt an integrated farming approach and emphasized that efficiency use of water,
energy and nutrients needs to be improved in maize to make it more climate-resilient.
The guest-of-honour on the occasion, Dr. J.S. Sandhu, Deputy Director
General (Crop Sciences), highlighted that hybrid breeding needs greater focus, and research on suitable maize germplasm with tolerance to both drought and
water-logging conditions is required. Dr Sandhu said maize is a unique crop and has multiple uses in terms of feed, food, fodder and fuel. ―The productivity of maize is highest in Tamil Nadu with 5.4 tonnes, Punjab - 4 tonnes, Haryana - 3
tonnes, Uttar Pradesh -1.8 tonnes,‖ he disclosed. In Punjab, Kapurthala, Hoshiapur, Patiala, Shaheed Bhagat Singh Nagar and Tarn Taran districts have
contributed significantly to the enhancement of maize productivity, he said. Calling maize a climate resilient crop, Dr Sandhu stressed on focusing on crop management as well as demand-supply.
In his presidential address, Dr. B.S. Dhillon, traced the history of maize breeding and called upon for greater infusion of science in maize research. Dr
Dhillon, while highlighting the achievements of PAU maize section, underlined the need for change in stereotype research and improvement in quality of
testing. Quality protein maize needs to be pushed, he said, while suggesting focusing on marker assisted selection and double haploid technology in maize breeding. Since maize is a promising crop for diversification, there is a need to
study its nutritional value too, he added. Dr Dhillon also emphasized on addressing the issue of climate variations, he added.
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Referring to Prime Minister, Narendra Modi’s ―Make in India‖ campaign, Dr A S Khera called upon the maize scientists to develop their own varieties and
have their own public-private partnership. He said major emphasis should be on maize and cotton as both the crops are wide spacing. In addition, attention
should also be paid to crop improvement, he added.
Dr Sharma observed maize research has undergone a sea change in the last few years. The challenges of genetic enhancement and strengthening of
seed production progarmme need to be addressed, he observed. He congratulated the maize scientists for the upgradation of Directorate of Maize
Research to Indian Institute of Maize Research.
Dr I.S. Solanki said maize is the third most important cereal crop of the country. Therefore, it should be popularized among farmers, industry and
consumers, he added. More thrust is being laid on single cross hybrids as they have shown resistance to various insect-pests and tolerance to drought, he
observed. Dr Solanki also stressed on setting-up of industries in the areas where maize is grown in volumes. Speaking on the occasion, he pointed out that maximum benefit from the hybrid technology is seen in maize.
Dr. O.P. Yadav, Director, IIMR reviewed the maize improvement work carried out during 2014-15 and informed that maize recorded the highest
production of 24.35 million tonnes during 2014-15 that has been made possible through flagship role of AICRP on maize. During last year, 17 new hybrids and
varieties were released for different states of country. According to the 12th Plan of ICAR, five new Centers on Maize are set to come up in West Bengal, Bihar, Maharashtra and in remote areas, he revealed.
Earlier, Dr Balwinder Singh, Director of Research, PAU while welcoming the dignitaries and the delegates, said the maize production has doubled in the
past few years and by 2025, its production is expected to escalate all the more. ―Grown in 160 countries, maize is the first crop to benefit from hybridization,‖ he highlighted. PAU has the credit of developing first single cross maize hybrid, he
disclosed, while adding the university has released 41 high yielding composites/hybrids of maize so far. The cultivation of sweet corn, baby corn and
quality protein maize can give additional income to the farmers, he added.
Dr H.S. Dhaliwal, Dean, College of Agriculture, proposed the vote of thanks. On the occasion, a souvenir and a CD on annual report of AICRPM 2014
were released. Besides, Best AICRP Centre Award was bestowed on Tamil Nadu Agricultural University, Punjab Agricultural University and a maize hybrid
producing firm. In addition, certificates were distributed for the protection of plant varieties and Farmers’ Rights Act. During this session, Dr. A.S. Khehra, Former Vice-Chancellor and Dr. B.M. Prasanna, Maize Geneticist were felicitated
for their contributions to maize research.
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Session II
Review of work during Kharif 2014 and Rabi 2013-14
Chairman Co-Chairman Rapporteurs
Dr. J.S. Sandhu, DDG
(CS), ICAR
Dr. I.S. Solanki,
ADG (FFC), ICAR
Drs. J.C. Sekhar, Meena
Shekhar, Ashok Kumar,
Chikkappa G.K.
Breeding
The session was started with presentation on promotions of entries in different
maturity groups namely late, medium, early and extra-early from IVT to AVT-I
and AVT-I to AVT-II. In the course of presentation several observations were
made and the following points were emerged after thorough discussion. The
details of the entries promoted were discussed and finalized.
The coordinated trials may be split into different sets comprising 30-40 entries
per set if number of test entries exceeds 60.
1. It was decided to include zone specific check(s), which were released and notified for the zone, while constitution of coordinated trials.
2. The percentage superiority over best check of the zone, which was released and notified for the zone in late maturity group was increased to 10% from the earlier 5% for promotion from IVT to AVT-I.
3. The separate session was organized for researchers involved in conducting trial at voluntary centres as well as fresh researches joined in the AICRP
centres to appraise them how to generate appropriate quality data while conducting coordinated trials.
4. The separate maize biofortification trial for different quality traits like pro-
vitamin A will be constituted and executed at specific locations from Kharif 2015 to encourage trait specific breeding.
Crop production
The findings of 11 experiments conducted at 27 centres were presented by Dr A
K Singh, PI (Agronomy). The following points were suggested:
In cropping system based trials, data of previous season’s crops should be
incorporated in the presentation, so that complete recommendations may be
made.
The experiments completed three years should be analyzed and summarized
recommendations may be made.
The trials on weed and water management must be formulated for coming
season. Herbicide molecules will be arranged by PI Agronomy.
For intercropping trials having pulses as a component crop, discussion may
be made with the IIFSR, Modipurum and IIPR, Kanpur before finalization.
Comprehensive information including economics should be considered for
presenting the results.
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Entomology
Dr. Pradyumn Kumar presented the results of Kharif 2014 and Rabi 2013-14
trials and summarized the results of resistant lines and hybrids identified for
Chilo partellus, Sesamia inferens in different coordinated trials. He presented the
incidence of cob borer complex, Helocoverpa armigera, Euproctis spp.,
Spodoptera in maize during grain filling stage across zones. Evaluation of bio-
control agents to control Chilo partellus and incidence of egg parasitoid and
larval parasitod, Cotesia on C. partellus in different zones was also presented. In
addition efficiency of different insecticides in controlling Chilo partellus along
with crop losses validation formulae was presented. Finally, he briefed on plant
volatile collection system for which a patent has been applied. He also presented
the information plant volatile collection system for which patent was filed.
Presentation ended with thanks to chair.
Pathology
Dr. KS Hooda presented the results of Kharif 2014 and Rabi 2013-14 trials and summarized the results of resistant lines and hybrids identified for different diseases in different coordinated trials. A total of 551 hybrids in both seasons
and 231 inbred lines (Kharif only) were screened against major diseases and Cyst nematode; and promising ones were identified. The avoidable yield loss in
Vivek Hybrid 5 at Almora due to TLB was 17.21% whereas Dhaulakuan centre recorded 35.98% yield loss due to MLB, CLS and BLSB. In addition, disease surveys were conducted at farmers’ fields in maize growing areas to assess
overall disease scenario during the crop season. Promising components for the management of disease and cyst nematode in maize were identified.
Session III
Panel Discussion - “Doubling maize production of India by 2025 –
Opportunities and challenges”
Chairman Co-chairman Rapporteurs
Dr. J.S. Sandhu, DDG (CS), ICAR
Dr B.M. Prasanna, Director Global Maize Programme, CIMMYT
Dr. I.S. Solanki, ADG (FFC), ICAR
Dr. Pranjal Yadava Dr. Chikkappa Karjagi
Dr. Bhupender Kumar
The session was chaired by Dr. J. S. Sandhu, DDG (Crop Science), ICAR and co-
chaired by Dr. B. M. Prasanna, Director, Global Maize Programme, CIMMYT. The
panellists included renowned scientists from public, private and international
organizations. The panellists deliberated about the top priorities that need to be
addressed in order to double the maize production of India in next 10 years.
Starting the deliberations, Dr. Usha Singh, RAU, Dholi emphasized intervention
thorugh government policy to provide farmers a remunerative price and also to
enhance quality of maize produce through mechanized harvesting with
community based driers, to bridge the yield gap (mainly kharif) by adopting
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improved agronomic practices, creation new demand through product
diversification and inclusion of maize foods in mid day meal.
Dr. Hans Bhardwaj, Pioneer Hibred International, attracted the attention to
develop common maturity grouping system in India on the lines of
internationally accepted system, suggested to measure progress in terms of
genetic gain, to use prediction tools in plant breeding and to increase the
precision of plots to capture data accurately.
Dr. Dheeraj Pant, Monsanto India Pvt Ltd suggested bridging the yield gap
through popularization of scientific package of practices among farmers along
with enhanced associated extension system, better management of disease,
insect and weeds. He was of the opinion that, presently herbicide use is not
adequate in maize-based farming systems in India.
Dr. Bajinder Pal, Bioseed Ltd suggested for identifying productivity limitation of
different zone since there are different productivity zones of maize in India. He
highlighted that there are no limitations in high yielding rabi production zone
except storage infrastructure. In medium yielding zone of South India, leaf
diseases, stalk rots and drought are the major limiting factors as 25-30% area in
this zone is under drought. In medium yield zone of north India, there are
problem of excess water and stalk rot. In the low yield zone of North (viz., UP,
Bihar, Jharkhand) there are no specific products and farmers of this region grow
hybrids recommended for other zones. Similarly, for hill areas there are no good
genotypes available. He suggested six priority areas to double the maize
production by 2015 viz., to frame structured policy for germplasm exchange,
participatory germplasm enhancement system, to develop breeding centre for
different areas, working with policy makers to ensure remunerative price for
maize farmers, need for continuous monitoring of new and emerging challenges
and efforts to make corn a profitable crop.
Dr B. S. Vivek, CIMMYT, India suggested for use of doubled haploids, heterotic
organisation of germplasm and strengthening QPM programme along with high
oil, pro vitamin A and other traits for end users.
Dr M. L. Jat, CIMMYT, India suggested farming systems approach and cautioned
that the rice wheat system should not be repeated, to provide complete value
chain from maize production, marketing, availability to consumption along with
formulation of diverse farm situations.
Dr J. S. Chawla, PAU, Ludhiana suggested for accelerated breeding using DH and
MAS.
Dr J. P. Shahi, BHU, Varanasi suggested to address abiotic stresses like excess
moisture, drought etc., diversification of maize types like sweet corn and baby
corn in peri-urban agriculture, economically viable seed production system
especially for kharif season and contract farming to provide farmers a
remunerative price.
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Dr G. Nalathambi, TNAU, Coimbatore suggested for appropriate management
technologies, input subsidies for seed and fertilizers, minimum wages to
labourers is required to stop rural migration and MSP regime needs to be
implemented more seriously, close working of agricultural scientists with
farmers, soil health and crop rotation needs to be specially strengthened.
Dr S. K. Guleria, AICRP Bajaura (H.P.) suggested to manage major diseases
bacterial stalk rots and BLSB along with making available of hybrid seeds from
public sector.
Dr Feroz Hussain, IARI, New Delhi suggested for structuring of germplasm based
on the heterotic pattern, introgressing QTLs for biotic, abiotic stresses as well as
nutritional quality traits in maize germplasm.
Dr O. P. Yadav, Director, IIMR, New Delhi suggested for diversification of
germplasm base and heterotic grouping of material, to increase the rate of
genetic gain through accelerated breeding, to develop stress resilient genotypes
for diverse ecologies of India, upscaling adoption of single cross hybrids in
different parts of India, strengthening public private partnerships and human
resource development along with finding a mechanism to absorb extra maize
production.
Dr S.K. Vasal eminent maize breeder focused on strength of maize crop and
drawn attention to adopt traits and technologies which have been very
successful in other cereal crops, like the use of dwarfing gene in maize. He
suggested using fundamental science in maize breeding, heterotic grouping and
developing pools and populations.
Dr S Dass stressed for bridging yield gap by pushing available technologies for
rapid adoption, mechanization of maize cultivation, quality hybrid seed
production supported with large scale seed driers at seed production hubs and
transgenics.
Dr B.S. Dhillon, Vice Chancellor, PAU, Ludhiana stressed for maize production
management, development of plant type for high density planting and genotypes
resistant to stresses, use of MAS, DH, offseason nurseries.
The session was opened for general discussion among the audience and
participants of the workshop. Various delegates highlighted different important
issues that were untouched by the panellist. Dr M.C. Kamboj, CCS HAU, Dr
Laxmikant, Kaveri Seeds, Dr I.S. Solanki, ADG (FFC), ICAR, Dr Vinay Mahajan,
IIMR, Dr Vijendar, Karnataka State Seed Corporation, Dr S.M. Khanorkar, GAU,
Dr K.H. Patel, AAU, Dr M.C. Wali, AICRP Arabhavi and others participated in the
discussion.
Dr B.M. Prasanna comprehended all suggestions made by different panellists in
five important strategies - the technology targeting, bringing more science in
breeding and increase the rate of genetic gain, translating genetic gain obtained
at experimental field to farmer’s fields on sustainable manner through transfer of
technology, focussing on seed producibility of maize hybrids and integration of
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socio economic, markets and institutions. He suggested to assess the
performance of scientists by using quantified genetic gain and impacts not by
publications alone.
Concluding the deliberations, Dr J. S. Sandhu highlighted several issues like
reduction of cost of hybrid seeds, use of temperate germplasm in regular
breeding programme, focussing on breeding abiotic stress tolerant genotypes
and mechanization of maize production system. The panel discussion was very
fruitful and all the panellists and the participants were optimistic and unanimous
about the motion that maize production of India can definitely be doubled by
2025.
Session IV
Variety Identification Committee meeting
During the 58th Annual maize workshop held at Punjab Agricultural
University,the variety identification committee (VIC) meeting was convened
under the chairmanship of Dr. J.S. Sandhu, Deputy Director General (Crop
Sciences), ICAR, New Delhi in the committee room, PAU, Ludhiana at 5:30PM on
April 4, 2015.
Following were present during the meeting:
1. Dr. J S Sandhu , Deputy Director General (CS): Chairman
2. Dr. I S Solanki, Assistant Director General (FFC) : member
3. Dr. Balwinder Singh, Director Research, PAU, Ludhiana: member
4. Dr Vijendra Sangam,Karnataka State Seed Corporation, Dharwad:
member
5. Mr Vivek V.Thakare, Maharashtra State Seeds Corporation., Mahabeej
Bhawan, Krishi Nagar, Akola (M.S.): member
6. Dr Sain Dass, Technical Advisor, Hybrid Crops, NSC, New Delhi : member
7. Dr Jasbir Singh Chawla, Sr.Maize Breeder, PAU, Ludhiana: member
8. Dr M.C.Wali, Sr.Maize Breeder , UAS, Dharwad : member
9. Dr G.Nallathambi, Sr.Maize Breeder TNAU, Coimbatore: member
10.Dr Shailandra Wararthe, Namdhari Seeds, Bangalore: member
11.Dr. O P Yadav: member-secretary
Resource persons:
1. Dr. Pradyumn Kumar: PI Entomology
2. Dr. KS Hooda: PI Pathology
3. Dr. Aditya Kumar Singh: PI Agronomy
4. Dr. Jyoti Kaul: PS( Breeding)
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Session V
Review of research results of individual AICRP centres for Kharif-2014
and Rabi 2013-14 and formulation of plan of work for Kharif- 2015 and
Rabi 2015-16 (Concurrent discipline-wise, centre-wise presentations of
significant results and progress report)
Concurrent session: Crop Improvement
Chairman Co-chairman Convener Rapporteurs
Dr. I. S.
Solanki, ADG,
(FFC), ICAR
Dr. R. K. Gumber,
ADR, (Crop
Improvement),
PAU, Ludhiana
Dr. O. P. Yadav,
Director, IIMR, New
Delhi
Drs. J. Kaul,
Bhupender Kumar,
Chikkappa G.K.,
Pranjal Yadava
24 centres from zone 1 to 5 have presented the progress and achievements
made at their centre with respect to number of trial conducted, hybrids
developed and contributed to AICRP system for Multi-location Yield Trials. They
are also presented information on germplasm received as well as shared
from/with other centres. The details of person who presented the centres report
are given below.
S. No Name of the centre Scientist 1. Almora
Crop Improvement Division, VPKAS Almora–263601, Uttarakhand
Dr R.K. Khulbe
2. Ambikapur RMD College of Agriculture and Research Station, Ajirma-497001 Chattisgarh
-
3. Arabhavi Agriculture Research Station, Arbhavi-591306, Karnataka
Dr. Mruthunjaya C. Wali Sr. Breeder & I/c
4. Bajaura CSKHPKV, HAREC, Bajaura, Distt. Kullu – 175 125, Himachal Pradesh
Dr. S.K. Guleria Breeder
5. Bahraich Crop Research Station, NDUA&T, Bahraich-271801, Uttar Pradesh
Dr M.V. Singh
6. Barapani ICAR Research Complex for NEH Region, Umaim, Meghalaya
Dr. J. P. Tyagi
7. Banswara Agricultural Research Station, Borwat Farm, Dahot Road Banswara-327001, Rajasthan
Dr. P Rokadia
8. Bhubaneswar Department of Plant Breeding & Genetic , College of Agriculture, OUAT Bhubaneswar-751003,Odisha
Sri Digbijaya Swain Breeder & I/c
9. Chhindwara JNKVV, Zonal Agriculture Research Station, Chhindwara-480001 Madhya Pradesh
10. Coimbatore Dr. G.Nallathambi
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S. No Name of the centre Scientist Department of Millets, Centre for Plant Breeding & Genetics, Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu
Breeder & I/c
11. Delhi Indian Agriculture Research Institute Pusa, New Delhi -110012
Dr. Firoz Hussain Sr. Scientist
12. Dholi Tirhut College of Agriculture, Dholi, Bihar
Dr. Ajay Kumar
13. Godhra Main Maize Research Station, Anand Agricultural University, Godhra, Panchmahals-389 001, Gujarat
Dr. S.M. Khanorkar Sr. Breeder & I/c
14. Gossaigaon Regional Agricultural Research Station, AAU, Gossaigaon, Telipara Dist. Kokrajhar-783360, Assam
Dr GC Bora
15. Hyderabad Maize Research Centre, ARI, ANGRAU, Rajendra Nagar Hyderabad - 500 030, Andhra Pradesh
Dr. Narasinha Reddy
16. Jhabua Zonal Agricultural Research Station, RVSKVV, Jhabua, Madhya Pradesh
Dr Mahendra Singh
17. Kangra Shivalik Agricultural Research and Extension Centre, Kangra-176001 CSKHPKV, Himachal Pradesh
Dr. Uttam Chandel Asst. Breeder
18. Kanpur Department of genetics and Plant Breeding, C. S. Azad University of Ag. & Tech., Kanpur-208002, Uttar Pradesh
-
19. Karimnagar Agricultural Research Station, Karimnagar, ANGRAU-505 001 Andhra Pradesh
Dr D Sharvani and K Sumalini
20. Karnal CCS HAU RRS Uchani, Karnal-132001, Haryana
Dr. M.C. Kamboj Asst. Breeder
21. Kolhapur Maharashtra Shahu Agricultural School Campus, Line Bazar Kasba-Bawada, Kolhapur-4166003, Maharashtra
Prof. S.R. Kulkarni Breeder & I/c
22. Ludhiana Maize Section, Deptt. of Plant Breeding, Genetics & Biotech, P.A. U. Ludhiana-141004, Punjab
Dr. Jasbir Singh Chawla Senior Maize Breeder
23. Mandya Zonal Agricultural Research Station, V.C. Farm, Mandya, Karnataka
Dr. Puttaramanaik Breeder
24. Pantnagar (Uttarakhand) Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar-263145, Uttrakhand
Dr. S.S. Verma Sr. Breeder
25. Ranchi Dept. of Plant Breeding & Genetics, BAU, Kanke, Ranchi-834 006 Jharkhand
Dr. (Ms.) M. Chakraborty Asst. Breeder
26. Srinagar KD Research Station, S.K.U.A.&T., Post Box.905,
Dr Zahoor Ahmed Dar
19
S. No Name of the centre Scientist Srinagar-190001, J&K Sr. Scientist
27. Udhampur Maize Research Centre (AICRP), SKUA & T-J, Sansoo, Behind 71 Sub Area Officers Mess, Via P.O. Garhi, Udhampur, J&K
Dr. R.S. Sudan Breeder
28. Udaipur MPUA&T, RCA, Udaipur-313001, Rajasthan
Dr. R.B. Dubey Breeder & I/c
29. Vagarai Maize Research Station, Tamil Nadu Agricultural University Vagarai-624613, Tamil Nadu
Dr. Ganeshan
30. Varanasi Institute of Agricultural Sciences, Banaras Hindu University Varanasi-221005, Uttar Pradesh
Dr. J.P. Shahi Prof. cum Sr. Breeder
The following suggestions were emerged based on the observation made during
the presentations.
1) Centres were suggested to develop large number of new cross combinations
every year along with identification of economical seed production ability of inbred / female parents of hybrids.
2) The centres whose hybrids are being used as national check in AICRP trials were asked to produce sufficient quantity of hybrids seed and supply the required quantity in time to IIMR.
3) The centres were suggested to mention or provide source from which their inbred lines have been are developed.
4) The thrust need to be given on developing new inbred lines by recycling the existing inbred line by making pedigree crosses to diversify the existing germplasm base.
5) Most of the centres were concerned about their financial scarcity with the respect to pay and contingency.
Concurrent Session: Crop Production
Chairman Co-chairman Convener Rapporteur/s
Dr Thakar Singh, Head Dept of
Agronomy, PAU, Ludhiana
Dr. M.L. Jat, Senior Cropping Systems
Agronomist, CIMMYT, New Delhi
Dr. A.K. Singh, PI Agronomy,
DMR, New Delhi
Drs. Ashok Kumar, S.L. Jat
and Mahesh Kumar
At the outset, Dr. A.K. Singh welcomed the chairman and co-chairman of the session. Chairman of the session, Dr. Thakar Singh also welcomed all agronomists and invited all the agronomists to present salient findings for the
rabi 2013-14 and kharif 2014. Co-Chairman Dr. M.L. Jat in his remarks emphasized that agronomic management is very critical and the responsibilities
for doubling and sustaining maize production is increased on agronomist shoulders which can be done by development of efficient crop production practices. The meeting was attended by 30 natural resource management
scientists. The findings of kharif 2014 and rabi 2013-14 were presented and following Scientist attended the session:
20
S. No.
Name of the centre Scientist
1. JNKKV, ZARS, Chhindwara Dr. V.K. Paradkar
2. K.D. Farm, SKAUAST, Srinagar Dr. Bashi Ahamad Alie
3. MPUAT, Udaipur Dr. Dilip Singh
4. ARS, Arbhavi, Belgaum, Karnataka Dr. S.S. Hallikeri
5. AAU, MMRS, Godhra Prof. K.H Patel
6. Maize Research Station, TNAU, Vagarai Dr. R. Karthikeyan
7. Maize Research Centre, ARI, ANGRAU, Hyderabad
Dr. (Ms.) D. Sreelatha
8. Agricultural Research Station, ANGRAU, Karimnagar
Dr. (Ms.) G. Manju Latha
9. College of Agriculture, OUAT, Bhubaneswar Ms. Pramila Naik
10. RRS Gosasingaon, Asom Mr. Binod Kalitha
11. College of Agriculture, GBPUAT, Pantnagar Dr. Amit Bhatnagar
12. College of Agriculture, GBPUAT, Pantnagar Dr. Veer Singh
13. PAU, Ludhiana Dr. Mahesh Kumar
14. CCS HAU Regional Research Station, Uchani, Karnal
Dr. Jitendra Kumar
15. CCS HAU Regional Research Station, Uchani, Karnal
Dr. Narendra Singh
16. CCS HAU, Seed Farm, Hisar Dr. Suresh Kumar
17. CRS NDUAT, Bahraich Dr. M.V. Singh
18. RMD College of Agriculture and Research Station, Ambikapur
Dr. S.K. Sinha
19. Dept. of Plant Breeding & Genetics, BAU, Kanke, Ranchi
Dr. C.S. Singh
20. Shivalik Agricultural Research and Extension Centre, Bajaura
Dr. Vinod Sharma
21. RVSKVV, Jhabua Dr. Mahendra Singh
22. MPKV, Kolhapur Dr. Rajendra M.Gethe
23. Tirhut College of Agriculture, Dholi Dr. Mritunjay Kumar
24. RMD College of Agriculture and Research Station, Ajirma, Ambikapur
Dr. A.K.Sinha
25. BCKV, Kalyani, Nadia(WB) Dr. Swapan K. Mukhopadhyay
26. Statistical Investigator, Directorate of Millets Development, Ministry of Agriculture, Jaipur
Mr. A. Ansari
27. ICAR-IIMR, New Delhi Dr. S.L.Jat
28. ICAR-IIMR, New Delhi Dr. C.M.Parihar
29. ICAR-IIMR, New Delhi Dr. Ashok Kumar
30. ICAR-IIMR, New Delhi Dr. A.K.Singh
However, the scientist of the Almora, Kangra, Jhabua, Kanpur, and Varanasi didn’t attend the meeting as well as not presented the findings while the findings
of Banswara and Delhi centre were sent to PI and responsible person presented. After presentation following points have emerged to further improve the agronomic research of the country:
21
In tillage experiments, soil must be sampled from three depths (0-5, 5-15 and 15-30 cm) for soil physical and chemical properties after three years
of experimentation. The BISA, Ludhiana trial on water management in maize will be part of
AICRP maize. The weather data must be supplied with crop data. Publications should be made from the experiments completed three
cycles. Herbicides molecules will be arranged by PI Agronomy for weed
management trial. Data should be submitted in prescribed format as per the decided
treatments only otherwise data will not be included in report
The official communication Emails are [email protected] and [email protected]
The last date for submission of research data will be up to 15th December for Kharif and up to 1st August for Rabi season. Data received after these dates will not be included in report.
The group thoroughly discussed the achievements made in last year to make some recommendations out of the experiments conducted. It was decided the consistent results of the two year multi-locations experiments must go to
farmers filed as technologies. In this regard, the group came out with two following recommendations
Under rain fed conditions residue retention @ 5 t/ha on the soil surface is
recommended for enhancing maize productivity by 9-29%.
The intercropping of soybean/black gram in maize grown during kharif
season is recommended for Zone I, III and V.
After this the group discussed the work plan for the kharif 2015 and rabi 2015-16 trial-wise. The group made many suggestions in the conduct protocols of the experiment and approved them unanimously in nine ongoing experiments. In
view to have better water and weed management the group formulated two new trials on these aspects for enhancing maize production. The data to be recorded in all trials were thoroughly discussed and finalized for each experiment. In all,
eleven trials formulated to conduct during kharif 2015 and Rabi 2015-16 for agronomy programme were as follows:
Trials to be continued during 2013-14 1. Performance of pre-release genotypes under varying nutrients levels 2. Nutrient management in maize-wheat-green gram cropping system under
different tillage practices 3. Nutrient management in rice-maize cropping system under different tillage
practices 4. Nutrient management in maize-chickpea/mustard cropping system under
different tillage practices 5. Nutrient requirement of maize genotypes under different cropping systems 6. Effect of planting density and nutrient management practices on the
performance of hybrids in kharif season 7. Effect of planting density and nutrient management practices on the
performance of hybrids in rabi season 8. Optimization of nutrient and plant geometry management in zero-till rabi
maize
22
9. Long term trial on integrated nutrient management in maize- wheat cropping system
New Trials formulated
1. Enhancing water-use efficiency in rainfed maize 2. Weed management in maize systems
The session was ended by vote of the thanks to chair and all the scientists.
Concurrent Session: Plant Pathology & Nematology
Chairperson Co-Chairman Convener Rapporteurs
Dr. Pushpinder Paul Singh, Prof. & Head, Dept. of Plant Pathology,
PAU, Ludhiana
Dr. V.K. Kaul, Professor (Nematology),
PAU, Ludhiana
Dr. K.S. Hooda, Pr. Scientist,
IIMR, New Delhi
Dr. Meena Shekhar and Dr. S.S. Sharma
The group meeting, comprising of maize pathologists from various parts of the country was held at 9.00 AM at COA, PAU, Ludhiana to review the results of
different trials conducted during kharif 2014/rabi 2013-14, as well as the formulation of technical programme for kharif 2015/rabi 2015-16. Dr. Hooda, convener, requested the chairman to take up review of research achievements of
maize pathology.
Following centers presented their achievements in the group meeting:
Sr.
No.
Centre Presented/
not presented
Sr.
No.
Centre Presented/ not
presented
1. Almora Dr. Chandersekhar C. 10. Godhra Dr. S.K. Singh
2. Arbhavi Dr. I.K. Kallappanavar 11. Hyderabad Dr. R. Ranga Reddy
3. Bajaura Dr. Rakesh Devlash 12. Karnal Dr. Vinod Kumar
4. Barapani Not presented 13. Ludhiana Dr. Harleen Kaur
5. Bhubaneshwar Not presented 14. Mandya Dr. Mallikarjuna N.
6. Coimbatore Dr. P. Renuka Devi 15. Medinapur Dr. C.K. Bhunia
7. Delhi Dr. Robin Gogoi 16. Pantnagar Dr Pradeep Kumar
8. Dhaulakuan Dr. Ashwani Kumar 17. Udaipur Dr. S.S. Sharma
9. Dholi Dr. Phool Chand
A total of 18 trials (17 in kharif 2014 and one in rabi 2013-14) were allotted to
identified hot spot locations viz., Bajaura, Almora, Dhaulakuan, Barapani (AVTs only) in Zone I; Ludhiana (rabi & kharif), Delhi, Karnal, Pantnagar in Zone II; Dholi, Midanapur (Rabi & kharif) and Bhubaneswar, in Zone III; Arbhavi (rabi &
23
kharif), Coimbatore, Mandya and Hyderabad (Rabi & kharif) in Zone IV and Udaipur in Zone V.
A total of 551 hybrids (rabi & kharif) and 231 inbred lines (kharif) were screened against Maydis leaf blight (MLB), Turcicum leaf blight (TLB), Banded leaf and
sheath blight (BLSB), Sorghum downy mildew (SDM), Rajasthan downy mildew (RDM), Curvularia leaf spot (CLS), Post-flowering stalk rots (PFSR), Common rust, Polysora rust, Bacterial stalk rot (BSR) and Cyst nematode.
Fifteen centres presented the results of the trials conducted at their respective locations whereas 2 centres viz.; Barapani and Bhubaneswar centres remain
unrepresented. No new disease was recorded in trap nursery from any location. However, the assessment of avoidable yield losses due to MLB, BLSB and CLS was conducted at Almora and Dhaulakuan centres. Maize disease survey and
surveillance was undertaken in maize growing areas of Bhubaneswar (Odisha), Ludhiana (Punjab), Almora (Uttarakhand), Pantnagar (Uttarakhand), Bajaura,
Dhaulakuan, Sirmour, Solan, Bilaspur, Hamirpur, Kangra, Una (Himachal Pradesh), Arabhavi (Karnataka), Udaipur (Rajasthan) and Godhra (Gujarat) during the year kharif 2014. Disease management trials including that of cyst
management were conducted by all the allotted centres.
The chairman appreciated the achievements made by scientists of representative
centres. The chairman expressed his concern that inoculum load in sick plot should be calculated before conducting trials. All the survey data should be
correlated with meteorological parameters. The disease management trials should include more organic options.
Based on the critical review, the following observations emerged out from the
group meeting.
A. Technical observations
1. A total of 62 genotypes out of 120 tested in IVT (late maturity) trial were resistant to more than two diseases.
2. A total of 23 genotypes out of 27 tested in AVT I and AVT II (late maturity)
trials were resistant to more than two diseases. 3. Twenty eight genotypes were moderately resistant to cyst nematode
(Heterodera zeae). Some of them are RMH-726, CMH 11-593, LMH 314, HT 51412607, JH 13121, JH 31607, CMH 11-584, DH 1405, DH 1401,CMH 12-697, CMH 12-691, AH-1320, X35D601, CP.999, DAS-MH-105, CMH 11-582,
CMH 11-617, CMH 11-629, EH-2214, VIVEK QPM9-C 4. A total of 96 inbred lines were identified with multiple disease resistance
(MDR) which can be utilized as potential donors for development of resistant hybrids.
5. Trifloxystrobin 25% + Tebuconazole 50% @ 0.05% as foliar spray is the best
in controlling BLSB (64.4%). 6. Carbendazim 12 WP + Mancozeb 63 WP @ 0.3% as foliar spray can control
MLB up to 40.7% 7. Tebuconazole@ 0.5ml/litre + R. serpentine leaves @ 10% as foliar spray can
control TLB (35.8%).
8. Propiconazole + Mancozeb as seed treatment @ 2+2 g/Kg seed + Foliar Spray @ 0.25% before flowering could control charcoal rot up to 65.4%.
9. Seed treatment with Metalaxyl+Mancozeb @ 0.25% and spray @ 0.25% can control sorghum downy mildew disease up to 92.5%.
24
10. Lantana Leaf at 2 q/ha and Neem cake at 2 q/ha were effective in control of nematode.
11. All the centres/co-operators will send the data in soft as well as in hard copy in prescribed format to PI latest by 15th December 2015 positively. It may
please be noted that data received after this date will not be included in the compilation of Annual Progress Report of AICRP on Maize.
The group also discussed and formulated the Technical Programme for kharif 2015/rabi 2015-16. In all, the group approved 26 trials (24 in kharif; 2 in rabi
season).
A. Trials to be continued during 2015-16:
1. Disease screening of IVT hybrids of four maturity groups (4 trials)
2. Disease screening of AVT hybrids of four maturity groups (4 trials) 3. Disease screening of specialty corn hybrids of all maturity groups
4. Screening of maize hybrids against cyst nematode (Heterodera zeae) 5. Disease screening of station maize hybrids against major diseases 6. Screening of inbred lines against major diseases of maize
7. Assessment of avoidable yield losses due to major diseases of Maize 8. Trap nursery trial for disease incidence
9. Survey and surveillance of maize diseases 10. Performance of the previous years’ resistant inbred lines of respective
centre 11. Efficacy of fungicides in control of maydis leaf blight 12. Efficacy of botanicals/bioagents in control of maydis leaf blight
13. Efficacy of newer fungicides in control of banded leaf and sheath blight 14. Efficacy of newer fungicides in control of common rust
15. Efficacy of bioagents, fungicide and potash in control of post flowering stalk rot
16. Efficacy of bioagents and fungicides in control of downy mildews
17. Disease screening of maize hybrids against diseases of rabi maize 18. Disease screening of maize inbred lines against diseases of rabi maize
B. New trials formulated: 1. Efficacy of resistance inducers in control of maize diseases
2. To observe the racial differences in Cochliobolus heterostrophus
causing maydis leaf blight
Group meeting ended with vote of thanks to the Chair and all scientists proposed
by Dr. Meena Shekhar, Principal Scientist, IIMR.
Concurrent session: Entomology
Concurrent
Session
Chairman Convener Rapporteur
Entomology Dr. Balwinder Singh ,
Director of Research
and Head, Dept of
Entomology, PAU
Dr. Pradyumn Kumar
Principal Investigator,
Entomology
Dr. J.C. Sekhar,
Dr. Jawala Jindal
25
The Entomologists presented the work done at their respective Centers. The
work was reviewed and discussed. The Chairman appreciated the uniformity of
the experiments conducted at all the centres flawlessly. He emphasized need to
update the IPM strategy incorporating the results of bio control agents and
insecticides studied in previous years at various AICRP centres for maize. The
Principal Investigator then discussed the plan of work with members of all the
centers for 2015-16.
The following scientists attended the group meeting.
1. Dr. J.C. Sekhar, Principal Scientist, DMR, WN Hyderabad
2. Dr. M.Lava Kumar Reddy, Principal Scientist, ANGARAU, Hyderabad
3. Dr. Jawala Jindal, Asstt. Professor, PAU, Ludhiana
4. Dr.Ravinder Kumar, Asst. Entomologist, PAU, Ludhiana
5. Dr. Sushant Mahadik, MPKU, Rahuri
6. Dr. Maha Singh, Asst Maize Entomologist, CCSHAU RRS, Karnal
7. Dr. A.K.Rai, Associate professor, Pusa, Bihar
26
Session VI
Lead Lecture
The speaker of the session Dr. B. M. Prasanna, was introduced by the Dr. O.P.
Yadav, Director IIMR, New Delhi. In his introduction, Dr. O. P. Yadav highlighted
the achievements made by Dr. B. M. Prasanna as a scientist at IARI, New Delhi
and also as Director, Global Maize Programme, CIMMYT. Dr. S. K. Vasal, Eminent
Maize Breeder, CIMMYT highlighted the importance of the topic chosen which is
very pertinent in the present context and invited Dr. B. M. Prasanna to start the
presentation on a important topic ―Enhancing maize genetic gains and breeding
efficiency: status and prospects‖. In the presentation Dr. B. M. Prasanna touched
important aspect of maize breeding to increase the productivity of maize. The
major areas of importance are.
1. Increasing the genetic gain in the important traits
2. Bringing more area under improved varieties
3. Increasing the rate of turn-over of improved varieties with newly
developed varieties/hybrids.
He stressed for multi-dimensional approach for enhancing the maize production
and productivity. He stressed for development of improved varieties with stress
resilient traits as well as enhanced nutritional value and their cultivation under
improved production and management conditions. He emphasized on application
of new tools and techniques like MAS, MARS, genomic selection with doubled
haploid technique to increase the genetic gains, germplasm diversification and
enhancement through itrogression using off-PVP lines to develop stress resilient
maize genotypes for both biotic and abiotic streses. High throughput and
precise field phenotyping techniques and rapid cycle genomic selection for
complex traits like prolificacy, high vigour, stay green, higher yield, year round
nurseries for disease phenotyping, agronomic performance, stress tolerance etc.
can be used for to increase heritability of complex traits and selecting stable
genotypes.
In order to achieve rapid progress, understanding and diversifying germplasm
base, Unlocking functional diversity by systematic characterisation of germplasm
existed in gene bank will play significant role in future as well. He highlighted the
contributions made SUWAN-I, DTP and DTP-W and La Posta Sequia (LPS)
populations for enhancing the yield and also efforts of USA under GEM
programme and SeeD Initiative of CIMMYT for diversifying the genetic base and
characterization. He draws parallel Indian efforts by taking examples of Parbat,
Ageti 76 composites.
Finally he highlighted the importance of routine use of breeding data
management system (BDMS), biometrics and bioinformatics to increases the
efficiency of breeding and reduces the time to impact.
27
Session VII
ICAR-CIMMYT Collaborative Research
The presentation was made by Dr. Chikkappa GK on the summary of results of
trials conducted during kharif 2014. Several suggestions were made, namely the
presentation of results needs to be improved substantially by highlighting the
details of entries with superior performance, the results of agronomy
experiments conducted under ICAR-CIMMYT collaborative programme needs to
be included, information on utilization of CIMMYT germplasm also needs to be
presented, there is need to repeat the evaluation of best entries. Dr. SK Vasal
stresses on the need to integrate and utilize the best performing germplasm
material in the regular breeding programme of the AICRP centres as well as
IIMR. He emphasized to procure the best germplasm of their importance like
disease resistant germplasm, drought or water logging tolerant germplasm, high
yielding hybrids and their parental lines from CIMMYT through sending seed
indent and it the responsibility of CIMMYT to made available such germplasm.
Dr. A.R. Sadananda, proposed the new trials for 2015-16 and it was suggested
that CIMMYT need to indicate the source germplasm from which the germplasm
have been developed while proposing the trials for screening or phenotyping for
different traits. It was also suggested that the AICRP centres and IIMR, New
Delhi need to handle pedigree population to increase the utilization of CIMMYT
germplasm in regular breeding programme by developing wide genetic base in
the form of synthetic and composite. The trials were finalized under ICAR-
CIMMYT work plan are as follows.
Chairman Co-
chairman
Speakers Rapporteurs
Dr. S.K. Vasal Dr. I.S.
Solanki
IIMR Scientist (Dr.
Chikkappa G.K.) and CIMMYT scientist (Dr. A.R.
Sadananda)
Drs. C.M. Parihar,
Chikkappa G.K., G.K.
Gill
28
Proposed Trials under ICAR-CIMMYT Collaborative Work Plan during Kharif 2015 and Rabi 2015-16
Trial Description / Objective Management Ent × Rep ×
Row
Centre
Disease phenotyping trials
CAT151 Multilocation phenotyping of advanced
CIMMYT-Asia lines for turcicum
TLB 75x2x1=150 Almora Mandya Arbhavi
CAT152 Multilocation phenotyping of advanced
CIMMYT-Asia lines for Fusarium stalk
rot
FSR 75x2x1=150 Ludhiana
(PAU)
CAT153 Multilocation phenotyping of advanced
CIMMYT-Asia lines for Macrophomina
stalk rot
MSR 70x2x1=140 Hyderabad
(WNC)
Arbhavi
CAT154 Multilocation phenotyping of advanced
CIMMYT-Asia lines for BLSB
BLSB 75x2x1=150 Dhaulakuan Delhi
(IIMR)
Pantnagar
CAT155 Multilocation phenotyping of advanced
CIMMYT-Asia lines for Downy Mildew
DM 75x2x1=150 Mandya Dhaulakaun
Breeding Nurseries
CAT156 F2s: High Methionine x QPM Lines (for
methionine enrichment of QPM) - Set 1
Optimum 35x1x15=525 Delhi (IARI)
CAT157 F2s: High Methionine x QPM Lines (for
methionine enrichment of QPM) - Set 2
Optimum 35x1x15=525 Ludhian
(IIMR)
CAT158 F2s: Elite QPM pedigree crosses for
development of new generation of elite
QPM lines
Optimum 35x1x15=525 Delhi
(IIMR)
CAT159 S3s: QPM conversion of elite non-QPM
lines
Optimum 50x2x1=100 Delhi
(IIMR)
Ludhiana
(PAU)
CAT1510 BC1S2s: Elite lines for enhancing
drought tolerance
Optimum 40x2x1=80 Ludhian
(IIMR)
29
Trial Description / Objective Management Ent × Rep ×
Row
Centre
CAT1511 F2s: Improvement of elite inbred lines
for agronomic performance
Optimum 148x2x1=296 Delhi
(IIMR)
Breeding Trials
CAT1512 Early Maturing QPM Hybrids Optimum 40x2x1=80 Srinagar Barapani
Ludhiana
(PAU)
CAT1513 Intermediate-Late Maturing QPM
Hybrids
Optimum 60x2x1=120 Ludhiana
(PAU)
CAT1518 Extra early maturing (advanced) yellow
lines test crosses from DT populations
Optimum 45x2x1=90 Godra
CAT1521 Extra early maturing (advanced) yellow
and white lines test crosses from
DTxWL back cross populations
Optimum 15x2x1=30
Karimanagr Godra
CAT1522 Extra early maturing (advanced) white
lines test crosses from DTxWL
biparental populations
Optimum 20x2x1=40
Karimanagr Godra
CAT1523 Extra early maturing (advanced) yellow
lines test crosses from DTxWL
biparental populations
Optimum 15x2x1=30
Karimanagr Godra
CAT1524 Medium maturing (advanced) yellow
and white lines test crosses from
DTXWL biparental populations
Optimum 20x2x1=40
Karimanagr Godra
CAT1527 Extra early maturing (advanced) yellow
and white lines test crosses from
DTxWL back cross populations
Water-logging 15x2x1=30
Dholi Begusarai
CAT1528 Extra early maturing (advanced) white
lines test crosses from DTxWL
Water-logging 20x2x1=40 Dholi Begusarai
30
Trial Description / Objective Management Ent × Rep ×
Row
Centre
biparental populations
CAT1529 Extra early maturing (advanced) yellow
lines test crosses from DTxWL
biparental populations
Water-logging 15x2x1=30
Dholi Begusarai
CAT1530 Medium maturing (advanced) yellow
and white lines test crosses from
DTXWL biparental populations
Water-logging 20x2x1=40
Dholi Begusarai
CAT1537 Extra early maturing (advanced) yellow
lines test crosses from DT populations
Drought 45x2x1=90 Godra
CAT1540 Extra early maturing (advanced) yellow
and white lines test crosses from
DTxWL back cross populations
Drought 15x2x1=30
Karimnagar Godra
CAT1541 Extra early maturing (advanced) white
lines test crosses from DTxWL
biparental populations
Drought 20x2x1=40
Karimnagar Godra
CAT1542 Extra early maturing (advanced) yellow
lines test crosses from DTxWL
biparental populations
Drought 15x2x1=30
Karimnagar Godra
CAT1543 Medium maturing (advanced) yellow
and white lines test crosses from
DTXWL biparental populations
Drought 20x2x1=40
Karimnagar Godra
31
ICAR-CIMMYT collaboration in Agronomy
1. Estimation of green house gas emission in permanent conservation
agriculture experiments at IIMR, Delhi
2. Precision-conservation agriculture for maximizing yield and nutrient use
efficiency while reducing environmental foot prints in maize wheat
cropping system of eastern IGP*
Background: The average productivity of kharif maize in Bihar is very poor (1.5
t/ha) due to poor agronomic management especially due to inadequate nutrient use
and improper crop establishment coupled with multiple abiotic stresses.
Technological solutions involving precision nutrient management layered with CA
based management are needed not only to help adapting maize systems to multiple
abiotic stresses in eastern Gangetic plains but also to improve productivity,
profitability while reducing environmental foot prints.
*The trial will be conducted at BISA, Pusa, Bihar supported by CIMMYT-CCAFS and with strategic partnership with RAU Pusa, Dholi centre of AICRP on Maize. The results will be presented in annual maize workshop
Technical program: Treatments:
S.
No
Tillage
practices
Nutrient rate Application
method
Application time
Maize Wheat
1 CT (FP) FFP FFP FFP FFP
2 PB Ad-hoc state recommendation
Ad-hoc state recommendation
(broadcast)
Ad-hoc state recommendation
Ad-hoc state recommendation
3 PB Ad-hoc state
recommendation (NPK)
Drilling 3 splits 3 splits
4 PB Ad-hoc state rec (NPK)-80% N in 2 splits, 3rd N
split based on GreenSeeker
Drilling 3 splits 3 splits
5 PB Nutrient Expert based NPK rates
Broadcast 3 splits 3 splits
6 PB Nutrient Expert based NPK rates
Drilling 3 splits 3 splits
7 PB Nutrient Expert based NPK
rates-80% N in 2 splits, 3rd N split based on
GreenSeeker
Drilling 3 splits 3 splits
Replication: 03 Plot size: >150 m2
32
Observations:
1. Growth and yield parameters and yield 2. Input use
3. Production economics 4. Nutrient use efficiency 5. Green House Gases (GHGs)
3. Designing portfolios of precision water* and nutrient management in conservation agriculture based maize-wheat production systems in a
sandy loam soils of western IGP&
*Sub-surface drip irrigation system. The irrigation water will be applied based on SMT Centers: &The trial will be conducted at BISA farm, Ladowal, Ludhiana, supported
by CIMMYT-CCAFS and with strategic partnership with PAU and IIMR. The results
will be presented in annual maize workshop
Objectives Optimize water and nutrient rates, time and method of application in CA
based maize-wheat system Improve system productivity, water and nutrient use efficiency in maize-
wheat system in sandy loam soil Reduce environmental foot prints of tillage, water and nutrient use. Capacity development of stake-holder on precision-conservation agriculture
Develop science based policy guide for sustainable intensification in western IGP
Treatments 1. No-N control- both residues removed (-R) 2. No-N control- 50% of maize stover and 30% of wheat residue retained (+R)
3. 50% recommended N -R 4. 50% of recommended N +R
5. 75% of recommended N-R 6. 75% of recommended N +R 7. 100% of recommended N-R
8. 100 of recommended N +R 9. 100% of recommended N under CT system (fresh beds for maize and wheat
on flat) –R 10.50% of recommended N –R (supplemented with GreenSeeker guided N at
Flowering stage in maize and Feekes 7/8 in wheat) 11.50% of recommended N +R (supplemented with GreenSeeker guided N at
Flowering
Replication: 03 Plot size:
Observations: 1. Growth and yield parameters and yield 2. Input use (water, nutrient, labour, energy etc)
3. Production economics
33
4. Nutrient use efficiency 5. Water use efficiency
6. Green House Gases (GHGs)
4. Development of algorithms for GreenSeeker optical sensor for precision N management in maize for India* *The trial will be conducted at IIMR, Delhi, BISA, Pusa & Ludhiana and IIFSR
Modipuram Treatments:
Maize hybrids: 10 (promising hybrids of different maturity and growth habits from the list below) N levels: 0, 30, 60, 90. 120, 150, 180, 210, 240, 270, 300
Replications: 02 Suggested hybrids
1. HQPM 1 (Late) 2. HQPM 5 (Late) 3. HQPM 4 (Late)
4. HM 9 (Medium) 5. HM 10 (Medium)
6. HM 4 (Medium) 7. PMH 1 (Late)
8. PMH 3 (Late) 9. BIO-9681 (Late) 10. SEED TECH 2324 (Late)
11. PMH 4 (Medium) 12. BIO-9637 (Medium)
13. PRAKASH (Early) 14. VIVEK QPM 9 (Early) 15. VIVEK HYbrid 21 (Early)
16. VIVEK HYbrid 43 (Early)
5. Strategic partnerships in SSNM (Nutrient Expert) x Tillage trials Joint capacity development events Joint publications
Researchers involved from CIMMYT-BISA: ML Jat, RK Jat, HS Sidhu, Tek
Sapkota
34
Session VIII
Breeder Seed Production, FLDs and Training Programmes
Breeder Seed Production report for the year 2014
Status of breeder indented, allocated and produced during the kharif 2014 was
presented in detail by Dr. Jyoti Kaul. A total of 44.66 quintals of breeder seed of 33
cultivars (parental lines of 16 hybrids and 17 composites) was indented by DAC,
Govt. of India. The indent was allocated to centers during the 57th Annual Maize
Workshop to the centers. A total of 27.68 quintals of breeder seed was produced
during kharif 2014. Some of the centers are taking up breeder seed production
during rabi 2014-15 and spring 2015. This year BSP-V received only from VPKAS,
Almora center.
Among the centers, IARI, New Delhi and CSAU, Kanpur did not communicate
Breeder seed reports consecutively for two years. Apart from the DAC indent IIMR,
Begusari center has produced 12.5 quintals of Breeder seed of one of the parents of
HQPM-1, HKI-163 during kharif-2014. During rabi 2014-15, the center gave a
projection of 40 quintals seed production of HKI-193-1 and HKI-163.
Dr. Kaul sensitized all the breeders and center In-charges about the
importance of BSP-I, II, III, IV and V reports. And, she also encouraged centers to
submit the reports in time in prescribed format. Dr. Narsimha Reddy (ANGARU,
Hyderabad) raised the issue of very low quantity indent of Priya Sweet Corn
composite. He suggested that such low quantity indents should not be made as it
poses problem in certification. Dr. Sain Dass’s views were in affirmation to Dr.
Reddy’s. Chairman reiterated the importance of timely submission of BSP reports.
Outreach program (FLDs and TSP)
Dr. Ashok Kumar presented a complete account of Front Line Demonstrations
(FLDs) on maize technologies taken up during rabi 2013-14, spring 2014 and kharif
2014 was presented by Dr. Ashok Kumar. A total of 3433 FLDs were conducted on
various production technologies including specialty maize.
The FLD results seemed very encouraging; in all the cases the FLD average
yield was higher than state average yield. Honorable chairman of the session Dr.
Solanki pointed out that in Rajasthan, spring season FLD yield was three times
(>300%) the state average but was just 3% higher during kharif. The Tribal Sub
Plan (TSP) was successfully done during the reporting period. A total of 10 trainings
were imparted to 396 tribal farmers. The farmers were also made aware of value
addition during the training program. Two field days were conducted under TSP at
Aterna and Manauli village of Haryana and one at RMR& SPC, Begusarai. Farmers
were also given farm inputs during the field days.
Dr. Kamboj raised the issue of timely availability of funds to conduct FLDs
without difficulty and requested for timely allocation and release of funds. The same
issue was also raised by few more centers.
35
BSP-1: indent allocation Year of production: 2015 Kharif
Given below is the BSP-1 in respect of maize parental lines and OPVs to be produced in the forthcoming Kharif
season (2015):
Final Indent of Breeder Seed for kharif 2016
CROP : Maize Hybrid Seed Quantity in Quintals Indent
allocation S. No.
Parental lines Year of release
CG MP MH NSC RJ NSAI UK WB Total
I MPUAT Udaipur
1 Pratap QPM H-1 (HKI193-1) 2013 0.08 7.00 7.08 MPUAT
Udaipur Pratap QPM H-1 (DMRQPM-106)
0.20 14.00 14.20
2 Pratap Hybrid-3 (M) 2013 3.00 3.00
Pratap Hybrid-3 (F) 6.00 6.00
II CCSHAU Karnal
3 HQPM-4 (F) HKI193-2 2010 4.00 4.00 CCSHAU
Karnal HQPM-4 (M) HKI161 2.00 2.00
4 HM-10 (HKH-1200) Female Line
HKI193-2
2008 0.22 0.22
HM-10 (HKH-1200) Male Line HKI 1128 0.08 0.08
5 HQPM-7 (HKI 161 (Male)) 2008 0.10 0.02 0.12
HQPM-7 (HKI 193-1 (Female)) 0.30 0.04 0.34
6 HQPM-5 (F) HKI163 2007 0.15 0.30 0.45
HQPM-5 (M) HKI161 0.05 0.10 0.15
7 HQPM-1-Female HKI193-1 2007 0.30 1.00 1.30
HQPM-1-Male HKI 163 0.10 0.30 0.40
III VPKAS Almora
8 Vivek Maize Hybrid-17 (FH-3186)
female line CM153
2005 0.07 0.07 VPKAS
Almora
Vivek Maize Hybrid-17 Male LINE CM
212
0.03 0.03
IV IARI Delhi
9 Pusa Extra Early Hybrid Makka -5 (AH-
421)( F) CM150
2004 0.16 0.16 IARI Delhi
Pusa Extra Early Hybrid Makka -5 (AH-
421)(M) CM151
0.08 0.08
10 PEHM-2 (F) CM137 0.22 0.22
36
Final Indent of Breeder Seed for kharif 2016
CROP : Maize Hybrid Seed Quantity in Quintals Indent
allocation S. No.
Parental lines Year of release
CG MP MH NSC RJ NSAI UK WB Total
PEHM-2 (M) CM 138 0.11 0.11
V RAU Dholi
11 Sakthiman-2 (F) CML176 2004 0.06 0.06 RAU Dholi
Sakthiman-2 (M) CML186 0.03 0.04 0.07
VI ANGRAU Hyderabad
12 DHM-117 female-LINE BML6 2010 0.12 6.00 6.12 ANGRAU
Hyderabad DHM-117 male –LINE BML7 0.06 3.00 3.06
VII ANGRAU Karimnagar
13 DHM-121 (F) BML45 2014 0.05 0.05
DHM-121 (M) BML6 0.03 0.03
VIII MPKV Kolhapur
14 RAJASHREE female -LINE 0.10 0.10 MPKV
Kolhapur RAJASHREE R-LINE 0.05 0.05
IX TNAU Coimbatore
15 COHM-8 (F) UMI 1201 2014 0.05 0.05 TNAU
Coimbator
e COHM-8 (M) UMI1230 0.03 0.03
16 COHM-9 (F) UMI 1205 2014 0.05 0.05
COHM-9 (M) UMI 1230 0.03 0.03
TOTAL : 49.71
Final Indent of Breeder Seed for kharif 2016
CROP: Maize variety Seed Quantity in Quintals Indent
allocation S. No. Variety Year CG MP RJ NSAI UP UK Total
I MPUAT Banswara
1 Pratap Kanchan-2 WC-236(Y) 2009 1.00 0.20 1.20 MPUAT
Banswara
II MPUAT Udaipur
2 PRATAP MAKKA-3 0.20 0.20 MPUAT
Udaipur
III GBPUAT Pant nagar
3 Pant Sankul Makka-3 (D131) 2008 0.20 0.20 GBPUAT
Pantnagar
IV VPKAS Almora
37
Final Indent of Breeder Seed for kharif 2016
CROP: Maize variety Seed Quantity in Quintals Indent
allocation S. No. Variety Year CG MP RJ NSAI UP UK Total
4 Vivek Sankul Makka-31(VL-103) 2008 0.20 0.40 0.60 VPKAS
Almora
V CSUAT Kanpur
5 Azad Kamal (R 9803) 2005 0.20 0.20 CSUAT
Kanpur
VI IARI Delhi
6 Pusa Composite-3 (Composite-85134) 2005 3.20 0.20 3.40 IARI Delhi
7 Pusa Composite-4(Composite-8551) 2005 0.20 0.20 0.40
VII JNKVV Chhindwara
8 JAWAHAR MAKAI-216 (JM-216) 2002 8.00 30.50 38.50 JNKVV
Chhindwara
VIII Gwalior
9 JVM-421 2.20 2.20 RVSKVV,
Jhabua
IX UAS Mandya
10 NAC 6004 2001 7.00 7.00 UAS Mandya
X BAU Ranchi
11 BIRSA MAKKAI-1 1996 0.30 0.30 BAU Ranchi
TOTAL : 54.20
Final Indent of Breeder Seed for kharif 2016
CROP : MAIZE FODDER Seed Quantity in Quintals
S. No. Variety Year KK MH NDD
B
NSC RJ NSAI Total
1 Pratap Makka Chari-6 2009 8.80 1.00 9.80
2 J-1006 (FODDER) 1992 3.00 0.80 28.00 31.80
3 AFRICAN TALL COMPOSITE 1983 0.24 2.40 8.55 16.00 7.00 34.19
TOTAL : 0.24 2.40 20.35 16.80 1.00 35.00 75.79
Abbreviations:CG: Chhattisgarh; MP: Madhya Pradesh; MH: Maharashtra; NSC: National Seeds
corporation; RJ : Rajasthan ; NSAI: UK: Uttarakhand; WB : West Bengal ; UP: Uttar Pradesh
38
Session IX
Germplasm exchange, registration & seed issues
This session was chaired By Dr. Sain Dass , Ex Project Director, IIMR and co-chaired by Dr. IS
Solanki Asstt. Director General (FFC), ICAR, New Delhi.The rapporteurs were Dr. Jyoti Kaul,
Dr. BS Gill and Dr. CK Karjagi.
Following presentations were made:
1. Dr. JC Sekhar made an exhaustive presentation on Germplasm Exchange by
IIMR.During his presentation he described the various activities undertaken during the
year to facilitate germplasm exchange under the aegis of IIMR including organizing the
germplasm day during which the breeders from AICRP (Maize) centres were invited to
select the material as per their requirement.
2. Dr. A.R. Sadananda from CIMMYT, Hyderabad presented the status of germplasm
exchange under the aegis of CIMMYT.He highlighted the exchange of accessions
between CIMMYT - AICRP (maize) centres including IIMR and private sector in India.
3. Dr. J Kaul, IIMR presented the procedure for registration of germplasm with NBPGR
and PPVFRA. She explained the routes that may be followed for filing applications . The
new format developed by NBPGR was also discussed . The requirements for registration
were deliberated upon and breeders impressed to follow time-lines.
The meeting ended with the vote of thanks to the chair
Session X
General discussion
Director Maize has announced that this time IIMR will conduct National
demonstration in large size plots and include all commercial hybrids of public
and private sector.
Benchmarking of variety/hybrids for promotion from IVT to AVT I and AVT II
must be there and this will be 10% increase over best check irrespective of
maturity group.
Disease incidence data must be included as promotion criteria.
Centres contributing too many entries so it has been decided that one centre
can contribute only two entries in IVT.
In AVT II Quality analysis will also be done for protein %, Starch, Methionine
and other quality parameters. Procedure for taking samples for quality
analysis will be communicated so that proper sample could be sent to
Biochemistry laboratory for quality analysis.
Choice of checks: Latest check will be used in each maturity group (hybrid
released in last 3 years) and out of which best check (Public/Private) will be
used.
39
QPM trials: Proper Seed samples are not sent for quality analysis, QPM and
non QPM mixture has been reported by Biochemist. So, it is requested to
send only selfed samples to IIMR for quality analysis.
Guidelines for conducting trials will be uploaded on IIMR website
Baby corn trials: visit website to know which traits you have to record.
IIMR will upload the guidelines for the list of traits for specialty corns.
It is required to assess all material for its nutritional status; it will be under
bio -fortification project.
Trials on Bio-fortification: Director asked CIMMYT and IARI to help in
conducting these trials. In this regard Dr. Feroz Hussain, IARI, New Delhi
presented how these trials will be conducted. He elaborated the importance
of Fe, Zn, Phytic acid, lysine, tryptophan and beta–carotene.
Selfing of 03 cobs/rep/genotype—enough seed will be available for analysis.
Issues in data handling:
Suggestions to AICR partners: Check data before sending to IIMR.
Visit IIMR website for the excel sheet which has been developed for
recording data, use that only.
Please check outliers also.
Observations of AICRP scientists Response/comments
Dr G Nallathambi On entry contribution by centres, the
two entry are too less, by this way public institutions contribution will be
affected. We should take samples for quality analysis from 4 or 5 centres only. How the seed production issues
for hybrid should be addressed?
Dr. O.P. Yadav Once the hybrid is released, Director
will write a letter to state seed corporations as well as to NSC for seed
production.
Dr S.M. Khanorkar
There is need to allow every centre to contribute minimum 3-4 entry. The
test hybrids can be tested at Farmers field or not. Can we use this data? Decoded list may be provided to the
centres in the month of January or February.
Dr. O.P. Yadav
Pre-released hybrids are not allowed in FLD though you can generate data
from farmer’s field. Decoded list cannot be provided till
IIMR receive data from each and every centre
Dr JS Chawla Regarding outliers: one hybrid is at 1st
position at one place and at 40th position at another place—it may be due to fault in the way the trial is
conducted at centres. When we are talking for PPP, private firms must be
asked for research funding also. Some conduct under rainfed
Dr. O.P. Yadav
It has been observed that within location there is much variation in data, if the trial mean is low as
compared to state average—data may not be considered.
Irrigated vs Rainfed: we must have guidelines for specific centres
40
Observations of AICRP scientists Response/comments
conditions—such data should not be included. Centres must implement
proper plan provided by IIMR Dr SK Guleria Variation in data, we must use
monitoring reports also while considering the data of a particular
location. The centres which are not having irrigation facilities , they must inform that they cannot conduct trials
or IIMR may not provide trials to such centres
Dr JS Chawla Coding of trials: IIMR is also in fray and contributing hybrids, who will
handle coding?
Dr Solanki: don’t irrigate the rainfed trials
Dr Khanoorkar: At flowering only irrigation is must
Dr IS Solanki, ADG(FFC) suggested doubling coding , One coding with IIMR
and second coding with selected AICRP centre. The pattern of wheat group
may also be adopted.
41
Session XI
Presentations of work plan 2015-16
Chairman Co-chairman Rapporteurs
Dr. I.S. Solanki,
ADG(FFC), ICAR
Dr. K.S. Tind, HOD,
PBG, PAU, Ludhiana
Drs. J.C. Sekhar, Meena Shekhar,
Ashok Kumar, Ramesh Kumar and
Chikkappa G.K.
Promotion of entries and technical programme-Crop improvement
In the kharif 2014, total of 414 test entries were evaluated in 15 different
breeding trials of various maturity group and types of corns
Of 414 entries, 374 were in normal and 40 were in specialty corns which
include QPM (12), sweet corn (13), baby corn (7) and popcorn (8).
Total of 396 test entries [IVT (297), AVTI (67), Specialty corns trials (32)]
were available for promotion from their current evaluation stage to next stage of testing, of which 87 were promoted.
Entries (Listed below) were promoted based on yield superiority over the best check (5 % in Late (AVT I) and specialty corns trials and 10% in Late
(IVT), medium, early and extra early). Further days to 50% silking was considered as another criteria of promotion besides yield superiority in Medium, early and extra early trials.
Entry in different Trials during Kharif 2015
Trials Zone Entry Name
IVT New entries will be received
AVT-I
Late
ZONE-I No trial
ZONE-II No test entry available
ZONE-III CMH12-663, GOLD 1166
ZONE IV IN 8569, NMH-1247, PM 14101L, DMRH1308, ADV 0990296, HT 51412616, PRMH-189, DAS-MH-106, KMH-
3981, Super 1177, IN 8570, DMH-192, NT 6325, IN 8902, IN 8602, ADV 1190384, CMH11-618, KH-2192, CMH10-555, CMH12-663, ADV 0990293, , GK-3118, 115-
08-01
ZONE V JH 13252, JH 13282, JH 13270, HT 51412607, JH 12010,
ADV 0990296, IN 8902, VNR 4325, KH-2192
Medium ZONE-I PM 14106M, HT 51412182, BH 412084,
ZONE-II CP.201
ZONE-III GK-3120, DAS-MH-306,
ZONE IV JH 31605, JKMH 4848, BL 897, PM 14107M, GK-3120
ZONE V No trial
42
Entry in different Trials during Kharif 2015
Trials Zone Entry Name
Early ZONE-I No trial
ZONE-II No trial
ZONE-III No trial
ZONE IV AH 9001, KDMH 100-1
ZONE V GYH-0656, AH 9001
Extra
Early
ZONE-I No trial
ZONE-II EH-2236 , AH-1317
ZONE-III No trial
ZONE IV No trial
ZONE V No trial
AVT-II
Late ZONE-I No test entry available
ZONE-II X35D601
ZONE-III No trial
ZONE IV HTMH 5108, X35D601, IM 8562
ZONE V DKC 9133(IM9133), X35D601, HTMH 5202, IM 8556, SIRI 4527, DAS-MH-105, CP.999, PRO-392, DKC 9141
(IM8539)
Medium ZONE-I No trial
ZONE-II No trial (AQH 4*)
ZONE-III No trial (AQH 9*)
ZONE-IV DKC 9144(IM8478), HTMH 5402 (AQH 8*)
ZONE V HT 51412607
Early ZONE-I Bio 9720
ZONE-II No trial
ZONE-III No trial
ZONE IV FH 3664
ZONE V JH-31613, FH 3664
Extra
Early
ZONE-I APQH 9*
ZONE IV APQH 9*
QPM Across the zone
BQPMH 36, VEHQ 14-1, BQPMH 36, LQPMH 314
BC Across the zone
BVM-2, ASKBH-1, CMH 11-659, CMH 11-658, NP 5040, NP 5004
SC Across the zone
ADVSW -1, ADVSW -2, FSCH 55, SWC 001 (Zone IV), QMHSC-1182, FSCH 41, ASKH 1, BSCH 6,BSCH 63
PC Across the zone
HPC1, DMRHP1402, DMRHP1401, KDPC-2,
*EDVs developed by MAS
43
The concerned centers must make availability of 40kg/check variety seed for the kharif 2015, 2016 in normal and 30kg in QPM and baby corn and 20kg in case of
sweet corn and popcorn check varieties. During rabi 2015-16, 25 and 20 kg check varieties seed should make available
in normal and QPM respectively. The list of checks was provided to each centre in the workshop. Should there be less number of entries in the AVT I and AVT II, trials can be
merged to constitute a common trial for effective evaluation of the entries. The seed submitted for testing in AICMIP must be un-treated.
The breeders must give full details of their hybrids submitted for testing under AICMIP. All breeders must confirm delivery of their entries at IIMR.
Technical programme for 2015-16
The advance trials for kharif 2015 will be constituted using promoted entries whereas initial varietal trials will be constituted with new entries received from
different centers and organizations.
The required quantity of seed material of promoted entries along with pedigree
details as per the given Performa should reach the office of the Director, Indian Institute of Maize Research, Pusa Campus, New Delhi-110012, latest by 15th May 2015 for kharif and by 10th October 2015 for rabi trials along with
required testing fee of Rs. 60,000 + 14 % service tax/entry/trial/year (Private Sector) in the form of DD in favor of Project Director (Maize), Indian Institute of
Maize Research, Pusa Campus, New Delhi, payable at New Delhi. Entries without fee and pedigree of hybrid/varieties will not be accepted. Seeds of each entry should be packed separately in cloth bag.
Initial varietal Trials (IVT-I) (Across the zones): No. of rows –
2 (net)
Advance varietal Trials-II (AVT-II) or (AVT I+II) (Zone specific): No. of
rows – 6 (net)
Row length – 4m (net) Row length – 4m (net)
Spacing- 75cm x 25cm -Rainfed, 60cm X20 cm in Irrigated
Spacing- 75cm x 25cm -Rainfed, 60cm X20 cm in Irrigated
Replications – 3 Replications – 3
Fertilizer – As per recommendations Fertilizer – As per recommendations
Advance varietal Trials-I (Zone specific): No. of rows – 4 (net)
QPM/SC/PC-I-II-III (Across the zone) : No. of rows – 4 (net)
Row length – 4m (net) Row length – 4m (net)
Spacing- 75cm x 25cm --Rainfed, 60cm X20 cm in Irrigated
Spacing- 75cm x 25cm -Rainfed, 60cm X20 cm in Irrigated
Replications – 3 Replications – 3
Fertilizer – As per recommendations Fertilizer – As per recommendations
Baby corn trials (BC-I-II-II):
(Across the zone) : No. of rows – 4 (net), Row length – 4m (net), Spacing- 60cmX 15cm, Replications – 3, Fertilizer – As per recommendations
44
Recommendation for Trials
AVT-I trials will be clubbed with AVT-II wherever the less number of entries are
available and will be allotted to new volunteer centers beside the regular. Different
trials will be planted with the following recommendation:
Observations to be recorded in initial and advance trials: Normal Maize
1. Days to 50% Flowering (Anthesis and silking)
2. Plant Height (cm) - Rounded to 0 decimal 3. Ear height/ placement (cm) - Rounded to 0 decimal
4. Days to maturity – 75% dry husk/appearance of black layer 5. Plant population at harvest (No./Plot)
6. Cobs count at harvest (No./plot) 7. Fresh cobs weight at harvest (Kg/plot) 8. Grain Moisture at the time of harvesting (%) - recorded in two replications
9. Shelling percentage (%) - recorded in two replications
Observations to be recorded in initial and advance trials: Quality Protein
Maize (QPM)
1. Days to 50% Flowering (Anthesis and silking)
2. Plant Height (cm) - Rounded to 0 decimal 3. Ear height/ placement (cm) - Rounded to 0 decimal
4. Days to maturity – 75% dry husk/appearance of black layer 5. Plant population at harvest (No./Plot) 6. Cobs count at harvest (No./plot)
7. Fresh cobs weight at harvest (Kg/plot) 8. Grain Moisture at the time of harvest (%) - should be recorded in two
replications 9. Shelling percentage (%) - recorded in two replications 10. Lysine and tryptophan (%) – recorded in the selfed cobs of two replications
Observations to be recorded in initial and advance trials: Popcorn
1. Days to 50% Flowering (Anthesis and silking)
2. Plant Height (cm) - Rounded to 0 decimal 3. Ear height/ placement (cm) - Rounded to 0 decimal
4. Days to maturity – 75% dry husk/appearance of black layer 5. Plant population at harvest (No./Plot)
6. Cobs count at harvest (No./plot) 7. Fresh cobs weight at harvest (Kg/plot) 8. Grain Moisture at the time of harvesting (%) - recorded in two replications
9. Shelling percentage (%) - recorded in two replications 10. Popping volume and percentage - recorded from selfed cobs
Observations to be recorded in initial and advance trials: Sweet corn
1. Days to 50% Flowering (Anthesis and silking)
2. Plant Height (cm) - Rounded to 0 decimal 3. Ear height/ placement (cm) - Rounded to 0 decimal
45
4. Green cob sweetness (%) (TSS) - should be recorded in 3 selfed cobs in each replication at 18-20 days after pollination
5. Plant population at harvest (No./Plot) 6. Green cob count at harvest – (No/plot)
7. Fresh green cob weight at harvest (Kg/plot)-with husk 8. Fresh green cob weight at harvest (Kg/plot)- without husk 9. Moisture percentage
Observations to be recorded in initial and advance trials: Baby corn
1. Days to 50% silking 2. Plant Height (cm) - Rounded to 0 decimal 3. Plant population
4. Baby corn height/ placement (cm) - Rounded to 0 decimal 5. Date of harvest of un-pollinated baby corn and number of baby corn from each
harvest (No./plot) 6. Fresh weight of baby corn per plot with husk (Kg) at each picking 7. Fresh weight of baby corn per plot without husk (Kg) at each picking
8. Length of baby corn (cm) 9. Diameter of baby corn (cm)
10. Total Fresh weight of baby corn per plot with husk (Kg) (sum of all pickings) 11. Total Fresh weight of baby corn per plot without husk (Kg) (sum of all pickings)
Quantity of seed of test entries required in each trial for kharif 2015
S.N. Trial Year of
testing
Seed quantity
(Kg)
Mode of conduct
1. Initial Varietal Trial (IVT) First 3.5Kg/Entry Across the zones
2. Advance Varietal Trial-I
(AVT-I)
Second 4.5
Kg/Entry/Zone
Zone specific
3. Advance Varietal Trial-
II(AVT-II)
Third 6 Kg/Entry/Zone Zone specific
4. Baby corn 1st
, 2nd
, 3rd
8Kg/ Entry Across the zones
5. QPM 1st
, 2nd
, 3rd
7Kg/ Entry Across the zones
6. Sweet corn 1st
, 2nd
3.5Kg/ Entry
3rd
(4.0Kg) *
Across the zones
7. Popcorn 1st
, 2nd
3.5Kg/Entry 3rd (4.0Kg) *
Across the zones
8. National Maize
Demonstration-Hybrids 1
st
0.5Kg/Hybrids New Delhi
9. National Maize
Demonstration-Inbreds
3rd 0.2Kg/Hybrids New Delhi
10. Grain Quality trials of released and AVT-II trials
hybrids
1st 1 Kg Selected centers
11. Check variety seed List attached
46
Quantity of seed of test entries required in each trial for Rabi 2015-16
S.N. Trial Year of
testing
Seed quantity (Kg) Mode of conduct
1. Initial Varietal Trail (IVT) First 3.5Kg/Entry Across the zones
2. Advance Varietal Trial-I
(AVT-I)
Second 3.5 Kg/Entry/Zone Zone specific
3. Advance Varietal Trial-
II(AVT-II)
Third 4.5 Kg/Entry/Zone Zone specific
4. QPM 1st
,2nd
,3rd
6 Kg/ Entry Across the zones
5. Check variety seed List attached
Quantity of seed of check variety required for constitution of Kharif 2015
and rabi 2015-16 trials
S. No. Check Name Maturity
Group
Centre Qty seed
(Kgs)
1. PMH 1 Late Ludhiana 40
2. PMH 3 Late Ludhiana 40
3. HM 11 Late Karnal 40
4. SeedTech2324 Late Bio Seed 40
5. Bio 9681 Late Bio Seed 40
6. PMH 4 Medium Ludhiana 40
7. Bio 9637 Medium Bio Seed 40
8. HM12 Medium Karnal 40
9. HM 8 Medium Karnal 40
10. HM 9 Medium Karnal 40
11. HM10 Medium Karnal 40
12. Prakash Early Ludhiana 40
13. PMH5 Early Ludhiana 40
14. Vivek Hybrid 21 Extra Early Almora 40
15. Vivek Hybrid 43 Extra Early Almora 40
16. Vivek QPM 9 QPM Almora 30
17. HQPM 1 QPM Karnal 30
18. HQPM4 QPM Karnal 30
19. HQPM 5 QPM Karnal 30
20. HQPM 7 QPM Karnal 30
21. HM 4 Baby corn Karnal 30
22. Madhuri Sweet corn Hyderabad 20
23. Priya Sweet corn Hyderabad 20
24. WOSC Sweet corn Hyderabad 20
47
S. No. Check Name Maturity Group
Centre Qty seed (Kgs)
25. VL Amber Popcorn Popcorn Almora 20
Normal Maize-Rabi 2015-16
S.
No.
Check Name Maturity Group Centre Quantity seed
(Kgs)
1. SeedTech2324 Late Bio Seed 25
2. Bio 9681 Late Bio Seed 25
3. Buland Late Ludhiana 25
4. Bio 9637 Medium Bio Seed 25
5. HM10 Medium Karnal 25
6. Prakash Early Ludhiana 25
Specialty Corns -Rabi 2015-16
7. HQPM 1 QPM Karnal 20
8. HQPM4 QPM Karnal 20
9. HQPM 5 QPM Karnal 20
10. HQPM 7 QPM Karnal 20
In addition to this, the details of newly added hybrids as checks from
IVT Kharif 2016, and Rabi 2015-16 are given below:
Newly checks hybrids from Initial Varietal Trial (IVT) Kharif 2016
Maturity Group
Check Name Centre /Company Seed quantity (Kgs)
Late CMH 08-287 TNAU, Coimbatore 10
CMH 08-282 TNAU, Coimbatore 10
BIO 9682 Bio Seed Research India
Ltd. Hyderabad
10
NK6240 Syngenta India Pvt. Ltd. 10
Medium CMH 08-292 TNAU, Coimbatore 10
BIO 9544 Bio Seed Research India Pvt. Ltd.
10
HM-12 CCSHAU, Uchani, Karnal 10
DHM 121 (BH 41009) ANGRAU, Hyderabad 10
Early PMH5 PAU, Ludhiana 10
BIO605 Bio Seed 10
DKC 7074 Monsanto 10
48
Newly checks hybrids from Initial Varietal Trial (IVT) Kharif 2016
Maturity Group
Check Name Centre /Company Seed quantity (Kgs)
Ex. Early Vivek Maize Hybrid 51 VPKAS, Almora 10
Vivek Maize Hybrid 45 VPKAS, Almora 10
QPM Pratap QPM Hybrid 1 MPUA & T, Udaipur 12
Sweet Corn Misthi Nuziveedu Seeds Limited 5
Note: Details of quantity seed mentioned here in newly added checks hybrids are based on the requirement for IVT-Kharif 2016 and Rabi 2015-16 only. The
quantity of seed required for next season will be increase and the same will be communicated in the workshop.
Newly checks hybrids from Initial Varietal Trial (IVT) Rabi 2015-16 Maturity
Group Check Name Company/Centre Name Seed quantity
(Kgs)
Late P3522 Pioneer Overseas Corporation 8
Medium BIO 9544 Bioseed Research India Pvt. Ltd.
8
HM10 CCSHAU, Uchani, Karnal 8
DHM 117 ANGRAU, Hyderabad 8
49
Maize Agronomic Trial (MAT) Technical programme Kharif 2015 and Rabi 2015-16
MAT 1. Performance of pre release genotypes under varying planting density and nutrient levels
Objective: To study the response of pre-release genotypes to different planting density and NPK levels with their interactions a) Performance of pre release early maturity genotypes in kharif under
varying planting density and nutrient levels in Zone I Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2
Sub-plot: Nutrient Levels (2) 150:50:60, 200:60:80 N: P2O5: K2O kg/ha. Sub-sub plot: Genotypes (1)+checks Design: Split-split plot
Replications: 3 Plot size: 12 m2
Locations: Almora, Bajaura, Udhampur, Gosaigaon b) Performance of pre release early maturity genotypes in kharif under
varying planting density and nutrient levels in Zone IV
Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2 Sub-plot: Nutrient Levels (2) 150:50:60, 200:60:80 N: P2O5: K2O kg/ha.
Sub-sub plot: Genotypes (1)+checks Design: Split-split plot
Replications: 3 Plot size: 12 m2 Locations: Arbhavi, Coimbatore, Kolhapur, Karimnagar, Hyderabad, Vagarai
c) Performance of pre release early maturity genotypes in kharif under varying planting density and nutrients levels in Zone V
Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2 Sub-plot: Nutrient Levels (2) 150:50:60, 200:60:80 N: P2O5: K2O kg/ha. Sub-sub plot: Genotypes (2)+checks
Design: Split-split plot Replications: 3
Plot size: 12 m2 Locations: Ambikapur, Chhindwara, Jhabua, Godhra, Banswara, Udaipur d) Performance of pre release medium maturity genotypes in kharif under
varying planting density and nutrients levels in Zone IV Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2
Sub-plot: Nutrient Levels (2) 200:65:80, 250:80:100 N: P2O5: K2O kg/ha. Sub-sub plot: Genotypes (2) +checks Design: Split-split plot
Replications: 3 Plot size: 12 m2
Locations: Arbhavi, Coimbatore, Kolhapur, Karimnagar, Hyderabad, Vagarai e) Performance of pre release late maturity genotypes in kharif under
varying planting density and nutrients levels in Zone II
Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2 Sub-plot: Nutrient Levels (2) 200:65:80, 250:80:100 N: P2O5: K2O kg/ha.
Sub-sub plot: Genotypes (1)+checks
50
Design: Split-split plot Replications: 3
Plot size: 12 m2 Locations: Delhi, Kanpur, Ludhiana, Karnal, Pantnagar
f) Performance of pre release late maturity genotypes in kharif under varying planting density and nutrients levels in Zone IV
Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2
Sub-plot: Nutrient Levels (2) 200:65:80, 250:80:100 N: P2O5: K2O kg/ha. Sub-sub plot: Genotypes (3)+checks
Design: Split-split plot Replications: 3 Plot size: 12 m2
Locations: Arbhavi, Coimbatore, Kolhapur, Karimnagar, Hyderabad, Vagarai g) Performance of pre release late maturity genotypes in kharif under
varying planting density and nutrients levels in Zone V Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2 Sub-plot: Nutrient Levels (2) 200:65:80, 250:80:100 N: P2O5: K2O kg/ha.
Sub-sub plot: Genotypes (9)+checks Design: Split-split plot
Replications: 3 Plot size: 12 m2
Locations: Ambikapur, Chhindwara, Jhabua, Godhra, Banswara, Udaipur h) Performance of pre release medium/late maturity genotypes in rabi
under varying planting density and nutrients levels in Zone II, III, IV &
V Main-plot: Density (2) 60 x 20 cm2 and 60 x 15 cm2
Sub-plot: Nutrient Levels (2) 200:65:80, 250:80:100 N: P2O5: K2O kg/ha. Sub-sub plot: Genotypes (as per promotion list)+checks Design: Split-split plot
Replications: 3 Plot size: 12 m2
Locations: Zone II: Delhi, Kanpur, Ludhiana, Karnal, Pantnagar Zone III: Bahraich, Kalyani, Varanasi, Bhubaneswar, Ranchi, Dholi
Zone IV: Arbhavi, Coimbatore, Kolhapur, Karimnagar, Hyderabad, Vagarai Zone V: Ambikapur, Chhindwara, Jhabua, Godhra, Banswara, Udaipur
Note: Split N application Nitrogen to be applied in three equal splits in all MAT 1(a to g) at basal, knee
high and tasseling stage in kharif season. Nitrogen to be applied in four equal splits in all MAT 1(h) at basal, knee high,
pre-tasseling and tasseling stage in rabi season.
Observations to be recorded in MAT 1(a to h): 1. Plant population at 25 DAS & at harvest (thousands/ha)
2. Plant height at harvest (cm) 3. Days to 50% tasseling 4. Days to 50% silking
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5. Number of cobs (thousands/ha) 6. 100-seed weight (g)
7. Grain yield at 15% moisture content (kg/ha) 8. Stover yield sun dry basis (kg/ha)
9. Insect-pest and disease incidence, if any 10. Net return and B:C ratio
i) Performance of pre release popcorn genotypes in kharif under varying
planting density and nutrients levels in Zone II Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2 Sub-plot: Nutrient Levels (2) 150:50:60, 200:60:80 and N: P2O5: K2O kg/ha.
Sub-sub plot: Genotypes (1) +checks Design: Split-split plot
Replications: 3 Plot size: 12 m2 Locations: Delhi, Kanpur, Ludhiana, Karnal, Pantnagar
j) Performance of pre release popcorn genotypes in kharif under varying planting density and nutrients levels in Zone III
Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2 Sub-plot: Nutrient Levels (2) 150:50:60, 200:60:80 and N: P2O5: K2O kg/ha. Sub-sub plot: Genotypes (2) +checks
Design: Split-split plot Replications: 3
Plot size: 12 m2 Locations: Bahraich, Kalyani, Varanasi, Bhubaneswar, Ranchi, Dholi k) Performance of pre release popcorn genotypes in kharif under varying
planting density and nutrients levels in Zone IV Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2
Sub-plot: Nutrient Levels (1) 150:50:60, 200:60:80 and N: P2O5: K2O kg/ha. Sub-sub plot: Genotypes (1) +checks
Design: Split-split plot Replications: 3 Plot size: 12 m2
Locations: Arbhavi, Coimbatore, Kolhapur, Karimnagar, Hyderabad, Vagarai l) Performance of pre release popcorn genotypes in kharif under varying
planting density and nutrients levels in Zone V Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2 Sub-plot: Nutrient Levels (2) 150:50:60, 200:60:80 and N: P2O5: K2O kg/ha.
Sub-sub plot: Genotypes (1) +checks Design: Split-split plot
Replications: 3 Plot size: 12 m2 Locations: Ambikapur, Chhindwara, Jhabua, Godhra, Banswara, Udaipur
Note: Nitrogen to be applied in three equal splits in all MAT 1(i to l) at basal, knee high and tasseling stage.
Observations to be recorded in MAT 1 pop corn (i to l):
1. Plant population at 25 DAS and at harvest (thousands/ha)
2. Plant height at harvest (cm)
52
3. Number of cobs (thousands/ha) 4. Days to 50% tasseling
5. Days to 50% silking 6. Popping (%)
7. 100-seed weight (g) 8. Grain yield (kg/ha) 9. Stover yield (kg/ha)
10. Insect-pest and disease incidence, if any 11. Net returns and B:C ratio
m) Performance of pre release sweet corn genotypes in kharif under
varying planting density and nutrients levels in Zone I, II & V Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2
Sub-plot: Nutrient Levels (2) 150:50:60, 200:60:80 and N: P2O5: K2O kg/ha. Sub-sub plot: Genotypes (3) +checks Design: Split-split plot
Replications: 3 Plot size: 12 m2
Locations: Zone I: Almora, Bajaura, Udhampur, Srinagar, Gossaigaon Zone II: Delhi, Kanpur, Ludhiana, Karnal, Pantnagar
Zone V: Ambikapur, Chhindwara, Jhabua, Godhra, Banswara, Udaipur n) Performance of pre release sweet corn genotypes in kharif under
varying planting density and nutrients levels in Zone IV Main-plot: Density (2) 60 x 20 cm2 and 50 x 20 cm2
Sub-plot: Nutrient Levels (2) 150:50:60, 200:60:80 and N: P2O5: K2O kg/ha.
Sub-sub plot: Genotypes (1) +checks Design: Split-split plot
Replications: 3 Plot size: 12 m2 Locations: Arbhavi, Kolhapur, Karimnagar, Hyderabad, Vagarai
Note: Nitrogen to be applied in three equal splits in all MAT 1(m and n) at basal, knee high and tasseling stage.
Observations to be recorded in MAT 1 sweet corn (m and n):
1. Plant population at 25 DAS and at harvest (thousands/ha)
2. Number of cobs (thousands/ha) 3. Plant height at harvest (cm) 4. Days to 50% tasseling
5. Days to 50% silking 6. TSS (Total soluble solids) at harvest (%)
7. Cob yield (kg/ha) 8. Green fodder yield (kg/ha) 9. Insect-pest and disease incidence, if any
10. Net returns and B:C ratio
o) Performance of pre release baby corn genotypes in kharif under varying planting density and nutrients levels in Zone I, II, IV & V
Main-plot: Density (2) 45 x 20 cm2 and 50 x 15 cm2
Sub-plot: Nutrient Levels (2) 150:50:60, 200:60:80 and N: P2O5: K2O kg/ha.
53
Sub- sub plot: Genotypes (3) +checks Design: Split-split plot
Replications: 3 Plot size: 12 m2
Locations: Zone I: Almora, Bajaura, Udhampur, Srinagar, Gosaigaon Zone II: Delhi, Kanpur, Ludhiana, Karnal, Pantnagar
Zone IV: Arbhavi, Kolhapur, Karimnagar, Hyderabad, Vagarai Zone V: Ambikapur, Chhindwara, Jhabua, Godhra, Banswara, Udaipur
p) Performance of pre release baby corn genotypes in kharif under varying planting density and nutrients levels in Zone III
Main-plot: Density (2) 45 x 20 cm2 and 50 x 15 cm2
Sub-plot: Nutrient Levels (2) 150:50:60, 200:60:80 and N: P2O5: K2O kg/ha. Sub- sub plot: Genotypes (2) +checks
Design: Split-split plot Replications: 3 Plot size: 12 m2
Locations: Bahraich, Kalyani, Varanasi, Bhubaneswar, Ranchi, Dholi
Note: Nitrogen to be applied in three equal splits in all MAT 1(p) at basal, knee high and tasseling stage.
Observations to be recorded in MAT 1 baby corn (p): 1. Plant height at harvest (cm) 2. Plant population at 25 DAS and at harvest (thousands/ha)
3. Days to first picking
4. Number of pickings 5. Baby corn yield with husk in each picking (kg/ha)
6. Baby corn yield without husk in each picking (kg/ha) 7. Green fodder yield (kg/ha) 8. Insect-pest and disease incidence, if any
9. Net returns and B:C ratio
MAT 2: Nutrient management in maize-wheat-green gram cropping system
under different tillage practices Objective: To find out effective SSNM and tillage practices for yield maximization in intensified cropping system
Tillage practices (Main-plots) Nutrient management (Sub-plots)
1. Zero till
2. Conventional Till 3. Permanent bed
1.RDF
2.SSNM based on nutrient expert 3.Farmer’s fertilizer practice
Design: Split Plot Replications: three Main-plot size: 150 m2 Locations: Udaipur, Pantnagar, Delhi, Dholi, Karnal, Banswara
Observations to be recorded: 1. Plant population (thousands/ha) in maize and mungbean at harvest
2. Effective tillers of wheat per square meter 3. Plant height (cm) at harvest of all crops 4. Yield attributes and yield of all crops
54
5. Days to reproductive stage of each crop 6. Days to maturity of each crop
7. System productivity 8. Stover/straw yields of all crops (kg/ha)
9. Net returns and B: C ratio 10.N, P and K uptake by all crops 11.Insect-pest and disease incidence, if any
12. Initial and final (after completion of three year cropping sequence) physical and chemical parameters of soil
MAT 3: Nutrient management in rice-maize cropping system under different tillage practices
Objective: To find out effective SSNM and tillage practices for yield maximization
in emerging cropping system
Tillage practices (Main-plots) Nutrient management (Sub-plots)
1. Zero till
2. Conventional Till 3.Permanent bed
1.RDF
2.SSNM based on nutrient expert 3.Farmer’s fertilizer practice
Design: Split Plot Replications: three Main-plot size: 150 m2 Locations: Dholi, Hyderabad Observations to be recorded:
1. Plant population (thousands/ha) in maize at harvest 2. Effective tillers of rice per square meter
3. Plant height (cm) at harvest of all crops 4. Yield attributes and yield of all crops 5. Days to reproductive stage of each crop
6. Days to maturity of each crop 7. System productivity
8. Stover/straw yields of all crops (kg/ha) 9. Net returns and B: C ratio 10.N, P and K uptake by all crops
11. Insect-pest and disease incidence, if any 12. Initial and final (after completion of three year cropping sequence) physical
and chemical parameters of soil
MAT 4: Nutrient management in maize-chickpea/mustard cropping systems under different tillage practices
Objective: To find out effective SSNM and tillage practices for yield maximization in maize-mustard/chickpea cropping system
Tillage practices (Main-plots) Nutrient management (Sub-plots)
1. Zero till
2. Conventional Till 3.Permanent bed
1.RDF
2.SSNM based on nutrient expert 3.Farmer’s fertilizer practice
Design: Split Plot Replications: three Main-plot size: 150 m2
Locations: Maize-chickpea cropping system-Banswara, Delhi Maize-mustard cropping system-Srinagar, Chhindwara, Delhi
55
Observations to be recorded: 1. Plant population in maize, chickpea and mustard at harvest
2. Plant height (cm) of all crops at harvest 3. Yield attributes and yield of all crops
4. Days to reproductive stage of each crop 5. Days to maturity of each crop 6. System productivity
7. Stover/straw yields (kg/ha) 8. Net returns and B: C ratio
9. N, P and K uptake by all crops 10.Insect-pest and disease incidence, if any 11. Initial and final (after completion of three year cropping sequence) physical
and chemical parameters of soil
MAT 5: Nutrient management for maize genotypes under different cropping
systems Objectives: 1. To find out SSNM practices for yield maximization of hybrids 2. To estimate the interaction of nutrient management with hybrids
Hybrids (Main-plots) Nutrient management (Sub-plots)
Five recommended hybrids
of the region
1.RDF
2.SSNM based on nutrient expert 3.Farmer’s fertilizer practice
Design: Split Plot Replications: three Sub-plot size: 15 m2
Locations:
Maize-wheat: Bajaura, Jhabua, Udaipur, Banswara, Ranchi, Ludhiana, Karnal, Delhi, Pantnagar
Maize-chickpea: Kanpur, Bahraich Maize-mustard: Chhindwara, Ambikapur, Srinagar Rice-maize: Hyderabad
Maize alone: Karimnagar, Arbhavi Observations to be recorded:
1. Plant population in maize and mungbean/chickpea /mustard at harvest (thousands/ha)
2. Effective tillers of wheat and rice per m2
3. Plant height (cm) of all crops 4. Yield attributes and yield of all crops
5. Days to reproductive stage of each crop 6. Days to maturity of each crop
7. System productivity 8. Stover/straw yields (kg/ha) 9. Net returns and B: C ratio
10.N, P and K uptake by all crops 11.Initial and final (after completion of one year cropping sequence) physical
and chemical parameters of soil. MAT 6: Effect of planting density and nutrient management practices on
the performance of hybrids in kharif season
Objective: To study the genotype x planting density x nutrient interactions for achieving higher yield in hybrid maize during kharif season
56
Locations Cropping system
Main-plot
(Hybrids:2)
Sub-plot
(Density:2)
Sub-sub plot
(Nutrient
mangement:3)
Zone I (Bajaura, Srinagar,
Gossaingaon)
Maize – Wheat/ Maize-mustard
Early/ medium
maturity
60 x 20 cm 60 x 15 cm
RDF STCR
SSNM
Zone II (Delhi, Ludhiana, Karnal,
Kanpur, Pantnagar)
Maize – Wheat Full maturity
67 x 20 cm 67 x 15 cm
RDF STCR
SSNM
Zone III (Dholi, Ambikapur, Bahraich, Bhubaneswar,
Varanasi, Ranchi)
Maize – Wheat/ Maize-mustard
Medium maturity
60 x 20 cm 50 x 20 cm
RDF STCR SSNM
Zone IV (Arbhavi, Hyderabad, Kolhapur,
Karimnagar)
Maize alone Full maturity
60 x 20 cm 50 x 20 cm
RDF STCR
SSNM
Zone V(Udaipur, Chhindwara, Banswara, Godhara)
Maize – Wheat/ Maize-Chickpea/
Maize-mustard
Early/ medium maturity
60 x 20 cm 50 x 20 cm
RDF STCR SSNM
Design: Split-split plot Replications: 3 Sub-sub plot size: 15 m2
Note: Select the hybrids from local market having maximum area at farmers filed and mention their names
Observation to be recorded: 1. Plant population at harvest in maize, mustard and chickpea (thousand/ha)
2. Barrenness in maize (%) 3. Effective tillers of wheat/rice per square meter 4. Plant height (cm) at harvest of all crops
5. Yield attributes and yield of all crops 6. Days to reproductive stage of each crop
7. Days to maturity of each crop 8. System productivity 9. Stover/straw yields of all crops (kg/ha)
10. Net returns and B: C ratio 11. N, P and K uptake by all crops
12. Insect-pest and disease incidence, if any 13. Initial and final (after completion of one year cropping sequence) physical
and chemical parameters of soil
MAT 7: Effect of planting density and nutrient management practices on
the performance of hybrids in rabi season Objective: To study the genotype x planting density x nutrient interactions for
achieving higher yield in hybrid maize during rabi season
57
Locations Cropping system
Main-plot (Hybrids:2)
Sub-plot (Density:2)
Sub-sub plot (Nutrient mangement:3)
Zone III
(Dholi, Bahraich)
Rice- Maize Full maturity 60 x 20 cm
50 x 20 cm
RDF
STCR SSNM
Zone IV
(Karimnagar, Vagarai)
Pulse-
maize/ Maize alone
Full maturity 60 x 20 cm
50 x 20 cm
RDF
STCR SSNM
Zone V (Banswara)
Soybean-maize
Full maturity 60 x 20 cm 50 x 20 cm
RDF STCR
SSNM
Design: Split-split plot Replications: 3 Sub-sub plot size: 15 m2
Note: Select the hybrids from local market having maximum area at farmers filed and mention their names
Observation to be recorded: 1. Plant population at harvest (thousands/ha) in maize, soybean and pulses
2. Barrenness in maize (%) 3. Effective tillers of wheat per square meter 4. Plant height (cm) at harvest of all crops
5. Yield attributes and yield of all crops 6. Days to reproductive stage of each crop
7. Days to maturity of each crop 8. System productivity 9. Stover/straw yields of all crops (kg/ha)
10. Net returns and B: C ratio 11. N, P and K uptake by all crops
12. Insect-pest and disease incidence, if any 13. Initial and final (after completion of one year cropping sequence) physical
and chemical parameters of soil
MAT 8: Optimization of nutrient and plant geometry management in zero-till rabi maize
Objective: Optimization of geometry and fertilization practices in ZT maize Location: Hyderabad Main-plot: Method of nutrient application
1. Farmer practice 2. Improved practice
Sub-plot: Nutrient management 1. Farmers practice 2. STCR
3. RDF Sub-sub plot: Planting density
1. 60 x 20 cm2 2. 50 x 20 cm2 3. 45 x 20 cm2
Design: Split-split plot Replications: 3 Sub-sub plot size: 15 m2
58
Observations to be recorded: 1. Plant population (thousands/ha) in maize
2. Barrenness in maize (%) 3. Effective tillers of rice per square meter
4. Plant height (cm) at harvest of all crops 5. Yield attributes and yield of all crops 6. Days to reproductive stage of each crop
7. Days to maturity of each crop 8. System productivity
9. Stover/straw yields of all crops (kg/ha) 10.Net returns and B: C ratio 11.N, P and K uptake by all crops
12.Initial and final (after completion of one year cropping sequence) physical and chemical parameters of soil.
MAT 9: Long term trial on integrated nutrient management in maize- wheat cropping system Objective: To assess the long term effect of integrated nutrient management in
maize wheat cropping system Location: Pantnagar
Treatment details:
T1 Control (Unmanured)
T2 100% RDF
T3 75% RDF
T4 50% RDF
T5 FYM 10t/ha + Azatobactor
T6 Maize + Cowpea with FYM 10 t.ha +Azatobactor
T7 100% RDF + 5 t/ha FYM
T8 50% RDF + 5 t/ha FYM
T9 100% RDF + 5 kg Zn/ha
T10 FYM 5 t/ha (state practice)
Design: RBD Replications: 3 Sub-sub plot size: 15 m2
Observations to be recorded: 1. Soil chemical, physical and biological parameters before start of the
experiment 2. Soil chemical, physical and biological parameters after completion of every
cropping cycle
3. Disease and insect incidence monitoring 4. Weed dynamics study
5. Plant population (thousands/ha) in maize at harvest 6. Effective tillers of wheat per square meter 7. Plant height (cm) at harvest of all crops
59
8. Yield attributes and yield of all crops 9. Days to reproductive stage of each crop
10. Days to maturity of each crop 11. System productivity
12. Stover/straw yields of all crops (kg/ha) 13. Net returns and B: C ratio of each crop and cycle 14. N, P, K and micronutrient content and uptake by crops
15. Insect-pest and disease incidence, if any
MAT 10: Weed management in maize systems
Objective: To develop weed management options in maize cropping system Locations: Maize-wheat: Pantnagar, Bajaura, Karnal, Ludhiana, Ranchi, Kalyani, Dholi,
Udaipur, Banswara Chhindwara, Behraich Maize-oat: Srinagar
Maize-mustard: Ambikapur, Bhubneshwar Rice-maize: Dholi, Kalyani, Gossaigaon Maize alone: Vagarai, Jhabua, Karimnagar, Arbhavi, Hyderabad
Treatment details:
T1 Control (weedy check)
T2 Weed free
T3 Atrazine @ 1.5* kg/ha preemegence
T4 Atrazine (750 g /ha) + Pendemathalin (750 ml/ha) preemegence
T5 Atrazine (750 g/ha) + 2,4-D Amine (500 g/ha) at 25 DAS as PoE
T6 Halosulfuron 60 g/ha at 25 DAS
T7 Atrazine @ 1.5 kg/ha preemegence fb Halosulfuron 60 g/ha 25 DAS
T8 Tembotrione (Laudis) 120 g/ha PoE at 25 DAS
T9 Pendemathalin (1000 ml/ha) preemegence fb Atrazine (750 g/ha) + 2,4-D Amine (500 g/ha) at 25 DAS as PoE
T10 Atrazine @ 1.5 kg/ha preemegence fb Tembotrione (Laudis) 120 g/ha PoE at 25 DAS
*For light soil Atrazine 1.0 kg/ha Design: RBD Replications: 3 Plot size: 15 m2
Observations to be recorded:
For crop: i. Plant population at 25 DAS and at harvest (thousands/ha) ii. No. of cobs (thousands/ha)
iii. Cob yield (kg/ha)
iv. Maize Grain yield (kg/ha)
v. System productivity as Maize equivalent yield vi. Insect pest and disease incidence, if any
vii. Economics: Net return and B:C ratio
60
For weeds:
i. No. of grassy, broadleaf weeds and sedges/m2
at 50 DAS and at harvest
ii. Weed dry matter at harvest/m2
of grassy, broadleaf weeds and sedges separately
iii. Phototoxic effects on crops, if any
MAT-11: Enhancing water-use efficiency in rainfed maize Objective: To enhance water productivity in rainfed maize
Locations: Maize-wheat: Dholi, Hisar Maize-mustard: Chhindwara, Bhubaneswar
Maize alone: Karimnagar, Srinagar Treatment details
Main-plot: Tillage practices 1. Conventional till 2. Conventional till + mulching
3. Zero tillage 4. Zero tillage + residue (4 t/ha)
Sub-plot: 1. Control (no hydrogel) 2. Hydrogel 2.5 kg/ha
3. Hydrogel 5.0 kg/ha
Design: Split -plot Replications: 3 Sub plot size: 50 m2
Observations to be recorded: 1. Plant population 25 DAS and at harvest (thousands/ha)
2. Plant height (cm) of maize 3. Days to 50% silking
4. Days to maturity 5. Yield attributes and yield of maize 6. Maize equivalents of crop and cropping system (kg/ha)
7. Stover yield of maize (kg/ha) 8. Net returns and B: C ratio
9. Moisture-use efficiency 10.Insect-pest and disease incidence, if any
61
Pathology and Nematology Technical Program for 2015-16
Maize pathological trials will be conducted at various coordinating/cooperating
centres during Kharif 2015 and Rabi 2015-16
A. Kharif 2015
MPT 1-9: Disease screening of IVT, AVT and specialty corn hybrids of all maturity groups (Tr no. 61-64; 75-78; Specialty Corn): Evaluation of maize hybrids of the coordinated trials (IVT, AVT I, AVT II and specialty
corn) consisting of four maturity groups against major diseases will be done under artificially/ sick plot created epiphyotics at following hot spot locations.
Hot spot locations:
S. No. Locations Diseases Total
1. Almora TLB 1
2. Arbhavi TLB, C.RUST 2
3. Bajaura TLB, 1
4. Barapani TLB 1
5. Coimbatore C. ROT 1
6. Delhi MLB, BLSB 2
7. Dhaulakuan BLSB, BSR 2
8. Dholi MLB 1
9. Hyderabad C.ROT 1
10. Karnal MLB, BLSB 2
11. Ludhiana MLB, C.ROT 2
12. Mandya TLB, SDM, P.RUST 3
13. Midnapur BLSB 1
14. Pantnagar BLSB, BSR 2
15. Udaipur BLSB, RDM, CLS, FSR 4
Total 26
List of susceptible checks
S. No. Check Diseases
1. CML 186, CM 600, CM 119, Local
Checks
Maydis leaf blight
2. Dhari, 219J, CM 202, Local Checks Turcicum leaf blight
3. CM 600, CM 501, Hishell, Local Checks
Banded leaf and sheath blight
4. CM 500, Local Checks Sorghum Downy mildew
5. Surya Rajasthan Downy mildew
6. Surya Curvularia Leaf spot
7. CM 600, DAC 7074, Local Checks Bacterial stalk rot
8. Surya, CM 500, Fusarium stalk rot
9. CM 600, 30V92, CM 501, Hishell Charcoal rot
10. CM 202, 219 J, Local Checks Polysora rust
11. Local Checks Common Rust
62
Observations: Record the disease screening data in following format: Season :
Replication :
Date of Sowing :
No. of Rows : Date of Inoculation :
Row Length :
Name of Sus. check : Date of Observation : Name of Resis. Check : Date of Harvesting :
S. No. Genotype R1 R2 Mean
1.
2.
3. Resistant Check
4. Susceptible Check
• Follow uniform method of disease screening under sick plot/ artificial
inoculated disease condition. If need be use Mckinney (1923) formula for calculating Percent Disease Index (PDI) [PDI = (Sum of individual ratings)/
(No. of leaves examined) ×100/ (Maximum disease rating)] mentioned in Annexure I.
• Overall mean score of foliar diseases of all plants in row should be recorded
by averaging of score of each plant. • Use susceptible and resistant checks at every 10th /20th row as per
availability of land. • Meteorological data of the centers during crop growth period should be
provided along with the disease reaction data. • Pathogen(s) of every disease should clearly be spelt out. • In case complex disease like PFSR (FSR, CR and late wilt), score of each
individual disease be mentioned. MPT 10: Screening of maize hybrids against cyst nematode (Heterodera
zeae) All the hybrids of IVT, AVT I, AVT II and Specialty corn will be screened against cyst nematode under sick plot condition.
Hot spot location: Udaipur; SC: Local check
Observations: Record the disease screening data in following format.
Season :
Replication : Date of Sowing :
No.of Rows :
Date of Inoculation :
Row Length :
Name of Sus. check : Date of Observation : Name of Resis. Check : Date of Harvesting :
S. no Genotype R1 R2 Mean
1.
2.
3. Resistant Check
4. Susceptible Check
MPT 11: Disease screening of station maize hybrids against major diseases
Hot spot locations: Dhaulakuan, Mandya, Ludhiana and Bajaura
63
Observation: Record the disease screening data in following format.
Season :
Replication :
Date of Sowing :
No. of Rows : Date of Inoculation :
Row Length :
Name of Sus. check : Date of Observation : Name of Resis. Check : Date of Harvesting :
S. no Genotype R1 R2 Mean
1.
2.
3. Resistant Check
4. Susceptible Check
MPT 12: Screening of inbred lines against major diseases of maize
All the inbred lines of normal and specialty corn will be screened at following
hot spot locations.
S. NO Locations Diseases Total
1. Almora TLB 1
2. Arbhavi TLB, Common rust 2
3. Bajaura TLB 1
4. IIMR C. ROT 1
5. Coimbatore SDM 1
6. Delhi (IARI) MLB, BLSB 2
7. Dhaulakuan BLSB, BSR, CLS 3
8. Dholi MLB 1
9. Hyderabad C. ROT 1
10. Karnal MLB 1
11. Ludhiana MLB, C. ROT 2
12. Mandya TLB, SDM, Polysora rust 3
13. Midnapur BLSB 1
14. Pantnagar BLSB, BSR 2
15. Udaipur FSR, RDM, CLS 3
Total 25
List of susceptible checks
S. no Check Diseases
1. CML 186, CM 600, CM 119, Local Checks
Maydis leaf blight
2. Dhari, 219J, CM 202, Local Checks Turcicum leaf blight
3. CM 600, CM 501, Local Checks Banded leaf and sheath blight
4. CM 500, Local Checks Sorghum Downy mildew
5. Surya Rajasthan Downy mildew
6. Surya Curvularia Leaf spot
7. CM 600, DAC 7074, Local Checks Bacterial stalk rot
8. Surya, CM 500, Fusarium stalk rot
9. CM 600, 30V92, CM 501 Charcoal rot
10. CM 202, 219 J, Local Checks Polysora rust
64
11. Local Checks Common Rust
Observations: As mentioned under disease screening trials of hybrids.
MPT 13: Assessment of avoidable yield losses due to major diseases of Maize
Trials on yield losses due to major diseases of maize will be conducted at following locations using paired plot technique with nine replications under sick plot/ artificially created epiphytotics.
Locations: Ludhiana (MLB, C. rot), Udaipur (RDM), Arbhavi (C. rot), Dhaulakuan (MLB) and Mandya (SDM)
Replication Treatment Disease score/
incidence (%)
PDI Yield (q/ha)
Yield loss in
(%)
R1 Protected
Unprotected
R2 Protected
Unprotected
. Protected
Unprotected
. Protected
Unprotected
R9 Protected
Unprotected
Mean
Disease control (%)
Avoidable yield losses (%)
CD (5%)
CV (%)
MPT 14: Trap nursery trial for disease incidence
The trial will be conducted to find out the occurrence of disease and/or any new disease on a set of maize inbred lines (10-12 lines) susceptible to disease at various locations. A special care has to be taken in observing
the incidence of viral diseases, if any.
Locations: Almora, Arabhavi, Bajaura, Barapani, Coimbatore, Delhi, Dhaulakaun,
Dholi, Hyderabad, Karnal, Ludhiana, Mandya, Pantnagar and Udaipur
Observations: Disease screening data should be recorded in following format:
S. No.
Plot No.
Pedigree (Code
No.)
Germination (%) Disease incidence
rating (Natural
condition)
Remarks
1.
65
• For soil borne diseases (PFSRs, BLSB, BSDM, SDM, RDM, cyst nematode), exact value of percent incidence should be mentioned. Additionally,
percent incidence should also be reflected in terms of mean disease score of foliar diseases and percent disease index (PDI) as mentioned in
Annexure I.
• For foliar diseases (MLB, TLB, CLS, rusts and viral incidence), scoring should be made in standard rating scale (Annexure I).
• Pathogen(s) of every disease should clearly be spelt out.
MPT 15: Survey and surveillance of maize diseases
During survey & surveillance of diseases, scoring should be done along with the incidence of disease in prescribed proforma.
Locations: Almora, Arabhavi, Bajaura, Barapani, Coimbatore, Delhi, Dhaulakaun,
Dholi, Godhra, Hyderabad, Karnal, Ludhiana, Mandya, Pantnagar and Udaipur
Observations:
• Mean disease score and procedure given for calculating PDI should strictly be followed.
• Pathogen of every disease should clearly be spelt out. Name and abbreviated form of diseases as given in Annexure I should uniformly be
followed.
• In case of viral diseases, vector population around the field and weather
factors to be recorded.
• Weather data of locations may be given with disease prevalence.
• Weather data should be recorded in following format and give its
correlation
S. No.
Station Name
Month
Temperatur
e (oC) Rainfall
of Month
(mm)
R.H (%) Sunshine
Hrs. Min Max Mi
n Max
MPT 16: Performance of the previous years’ resistant inbred lines of
respective centre
S.
No.
Centre Disease(s) S.
No.
Centre Disease(s)
1. Almora TLB 8. Hyderabad CR
2. Arbhavi TLB, CR 9. Karnal MLB, BLSB, C.rust
3. Bajaura TLB 10. Ludhiana MLB, CR
4. Coimbatore SDM 11. Mandya TLB, SDM, P.rust
5. Delhi (IARI) MLB,BLSB 12. Pantnagar BLSB, BSDM,
66
BSR
6. Dhaulakuan MLB, BLSB, BSDM, BSR
13. Udaipur RDM, CLS, FSR
7. Dholi MLB, TLB
MPT 17: Efficacy of fungicides in control of maydis leaf blight (MLB)
Location(s): Karnal
Objective: To identify promising fungicide(s) for management of MLB
Observations: Disease data should be recorded in following format:
Season :
No. of Rows :
Date of Sowing :
Row Length : Date of Inoculation :
Date of Observation :
Test Hybrid : Date of Harvesting : Replication :
Treatment Location
Mean disease
score
PDI* Disease control
(%)
Yield (q/ha
)
Yield increase (%)
T1 Propiconazole @
0.1 %
T2 Hexaconazole @
0.1%
T3 Carbendazim @
0.1%
T4 Mancozeb @
0.2%
T5 Carbendazim 12
WP + Mancozeb 63 WP @ 0.3%
T6 Untreated check (water spray)
T7 SEM+
T8 CD (0.05)
T9 CV (%)
67
MPT 18: Efficacy of botanicals/bioagents in control of maydis leaf blight (MLB)
Locations: Karnal
Objective: To identify of promising botanical/bioagents for management of MLB
Observation: Disease data should be recorded in following format:
Season :
No. of Rows : Date of Sowing :
Row Length :
Date of Inoculation :
Date of Observation : Test Hybrid : Date of Harvesting :
Replication :
MPT 19: Efficacy of newer fungicides in control of banded leaf and sheath blight
(BLSB)
Locations: Almora, Karnal, Ludhiana, Delhi, Dhaulakuan, Bajaura, Godhra,
Pantnagar
Objective: Identification of promising fungicide(s) for management of BLSB
Observation: Disease data should be recorded in following format:
Season :
No. of Rows : Date of Sowing :
Row Length :
Treatment Location
Mean
disease score
PDI
*
Disease
control (%)
Yield
(q/ha)
Yield
increase (%)
T1 Azadirachtin 3000ppm @ 0.3%
T2 R. serpentine leaves (Sarpgandaha) @10%
T3 A. marmelos leaves (Bel Pathar) @25 %
T4 TH-3 @ 0.5% as seed treatment, bioagent-
fortified
T5 TV-3 @ 0.5% as seed
treatment, bioagent-fortified FYM (1:50) and spray@ 0.5%
T6 Untreated check (water spray)
T7 SEM+
T8 CD (0.05)
T9 CV (%)
68
Date of Inoculation :
Date of Observation : Test Hybrid : Date of Harvesting :
Replication :
* Test Variety Name * Variety Name
* Transformed values in parenthesis
MPT 20: Efficacy of newer fungicides in control of common rust
Locations: Karnal, Arbhavi
Objective: To identify promising fungicide (s) for management of common rust
Observation: Disease data should be recorded in following format:
Season :
No. of Rows : Date of Sowing :
Row Length :
Date of Inoculation :
Date of Observation : Test Hybrid : Date of Harvesting :
Replication :
Treatment Location
Mean disease score
PDI* Disease control
(%)
Yield (q/ha)
Yield increase (%)
T1 Difenconazole @ 0.1 %
T2 Hexaconazole @ 0.1%
T3 Carbendazim @ 0.1%
T4 Validamycin @ 0.1%
T5 Tebuconazole @
0.05%
T6 Trifloxystrobin 25% +
Tebuconazole 50% @ 0.05%
T7 Azoxystrobin @ 0.05%
T8 Pencycuron @ 0.1%
T9 Untreated check
(water spray)
SEM+
CD (0.05)
CV (%)
69
* * Test * * Variety Name * Transformed values in parenthesis
MPT 21: Efficacy of bioagents, fungicide and potash in control of post flowering
stalk rots (PFSR)
Locations: Ludhiana (C.ROT), Arbhavi (C.ROT), Udaipur (FSR)
Objective: To identify promising component(s) for management of PFSR
Observation: Disease data should be recorded in following format:
Season :
No. of Rows :
Date of Sowing :
Row Length : Date of Inoculation :
Date of Observation :
Test Hybrid : Date of Harvesting :
Replication :
Treatments
Mean
disease score
PDI
Disease
control (%)
Grain yield
(q/ha)
Increase
over control
(%)
T1 TH -3 @ 0.5% as seed treatment, bioagent-fortified
FYM (1:50) and spray@ 0.5%
T2 Pseudomonas fluorescens @
0.5% as seed treatment, bioagent-fortified FYM (1:50)
Treatment Location
Mean
disease score
PDI*
Disease
control (%)
Yield (q/ha)
Yield
increase (%)
T1 Difenconazole @ 0.1 %
T2 Hexaconazole @ 0.1%
T3 Tebuconazole @ 0.05%
T4 Propiconazole @ 0.1%
T5 Trifloxystrobin 25% + Tebuconazole 50% @
0.05%
T6 Azoxystrobin @ 0.05%
T7 Inoculated Control
T8 Untreated check
(water spray)
SEM+
CD (0.05)
CV (%)
70
and spray@ 0.5%
T3 Local strains of fungal antagonists @ 0.5% as seed
treatment, bioagent-fortified FYM (1:50) and spray@ 0.5%
T4 Spraying of muriate of potash @ 1-2% at 30 days after planting
T5 Propiconazole @ 0.1% spray at 40 DAS
T6 Double dose of muriate of potash at 45 DAS
T7 Untreated check (water spray)
SEM+
CD (0.05)
CV (%)
Note: Incubate bioagent fortified FYM under moist condition at least for 10 days
before sowing of experiment.
MPT 22 Efficacy of bioagents and fungicides in control of downy mildews (SDM
and RDM)
Locations: Mandya (SDM), Udaipur (RDM)
Objective: To identify promising components for management of downy mildews (SDM and RDM)
Observations: Disease data should be recorded in following format:
Season :
No. of Rows : Date of Sowing :
Row Length :
Date of Inoculation
:
Date of Observation
:
Test Hybrid
:
Date of
Harvesting
:
Replication :
Treatments Incidence
(%)
Disease control
(%)
Grain yield
(q/ha) Increase
(%)
T1 Bacillus amyloliquefaciens
@10g/kg as seed treatment, bioagent-fortified FYM (1:50) and
spray @ 1.0%
T2 TH-3 @ 0.5% as seed treatment,
bioagent-fortified FYM (1:50) and spray @ 0.5%
T3 TV-3 (Trichoderma viride) @ 0.5% as seed treatment, bioagent-fortified FYM (1:50) and
71
spray @ 0.5%
T4 Fosetyl-al @ 0.2% seed treatment and spray @ 0.2%
T5 Azoxystrobin @ 0.2% seed treatment and spray @ 0.15%
T6 Metalaxyl+Mancozeb @ 0.25% seed treatment and spray @
0.25%
T7 Metalaxyl @ 0.25% seed treatment and spray @ 0.25%
T8 Untreated check (water spray)
SEM+
CD (0.05)
CV (%)
Note: Incubate bioagent fortified FYM under moist condition at
least for 10 days before sowing of experiment.
MPT 23: Efficacy of resistance inducers in control of maize diseases
Locations: Almora, Arabhavi, Bajaura, Coimbatore, Delhi, Dhaulakaun, Karnal, Ludhiana, Mandya, Pantnagar and Udaipur
Objective: To identify promising resistant inducer(s) for management of downy
mildews (SDM and RDM)
Observation: Disease data should be recorded in following format:
Season :
No. of Rows : Date of Sowing :
Row Length :
Date of Inoculation :
Date of Observation :
Test Hybrid : Date of Harvesting : Replication :
Treatments
Disease
mean Score
PDI
Disease
control (%)
Grain yield
(q/ha)
Increase
over control
(%)
T1 Salicylic acid (SA) @ 100
µg/g
T2 Salicylic acid (SA) @ 150
µg/g
T3 Salicylic acid (SA) @ 200
µg/g
T4 Salicylic acid (SA) @ 250 µg/g
T5 Acibenzolar- S-methyl (ASM) @ 100 µg/g
T6 Acibenzolar- S-methyl (ASM) @ 150 µg/g
T7 Acibenzolar- S-methyl
72
(ASM) @ 200 µg/g
T8 Acibenzolar- S-methyl (ASM) @ 250 µg/g
T9 Untreated check (water spray)
SEM+
CD (0.05)
CV (%)
MPT 24: To observe the racial differences in Cochliobolus heterostrophus causing maydis leaf blight (MLB)
Locations: Delhi, Dhaulakuan, Dholi, Karnal, Mandya, Udaipur
Objectives: To record the racial difference in Cochliobolus heterostrophus
Observations: Record the racial differences in Cochliobolus heterostrophus in set
of susceptible lines under artificially created MLB epiphytotics at each location.
B. Rabi 2015-16
MPT 25: Disease screening of maize hybrids against diseases of rabi
maize
Evaluation of maize hybrids of the coordinated trials of rabi maize against major diseases will be done under artificially created epiphyotics
at following hot spot locations.
Hot spot locations:
S. No. Locations Diseases Total
1. Arbhavi C. rot 1
2. Dholi TLB 1
3. Hyderabad C. rot 1
4. Karnal C. rust 1
5. Ludhiana C. rot 1
6. Mandya TLB, P. rust, SDM 3
List of susceptible checks
S. No. Check Diseases
1. Dhari, 219J, CM 202, Local Checks Turcicum leaf blight
2. CM 500, Local Checks Sorghum Downy mildew
3. CM 600, 30V92, CM 501, Hishell,
Local Checks
Charcoal rot
4. CM 202, 219 J, Local Checks Polysora rust
Observation: Record the disease screening data in following format :
Season :
Replication :
73
Date of Sowing :
No. of Rows : Date of Inoculation :
Row Length :
Name of Sus. check : Date of Observation : Name of Resis. Check : Date of Harvesting :
S. No. Genotype R1 R2 Mean
1.
2. Susceptible Check
Resistant Check
MPT 26: Disease screening of maize inbred lines against major diseases of
maize
S.
No.
Locations Diseases Total
1. Arbhavi C. rot 1
2. Dholi TLB 1
3. Hyderabad C. rot 1
4. Ludhiana C. rot 1
5. Mandya TLB 3
List of susceptible checks
S. No. Check Diseases
1. CM 600, 30V92, CM 501, Hishell, Local Checks
Charcoal rot
2. Dhari, 219J, CM 202, Local Checks TLB
Observation:
• Record the disease screening data in following format :
Season :
Replication :
Date of Sowing :
No. of Rows : Date of Inoculation :
Row Length :
Name of Sus. check : Date of Observation : Name of Resis. Check : Date of Harvesting :
S. No. Genotype R1 R2 Mean
1. 2. Susceptible Check
Resistant Check
74
Annexure I
Guidelines for Uniform Method of Disease Assessment in Maize
Under Artificially/ Sick Plot Created Epiphytotics The screening techniques and rating of the disease intensities for uniform
assessment of maize diseases are given below:
1. Turcicum leaf blight (TLB) and maydis leaf blight (MLB)
Sorghum grains soaked in water in a conical flask, autoclaved twice, seeded with
fungus under aseptic condition are kept for incubation at 25-270C. The flasks are shaken once in 2-3 days to facilitate uniform growth on grains. After 10 days the
material is ready for inoculation. Prepare a fine powder of impregnated sorghum grains after shade drying. Put a pinch of this powder in the leaf whorl of 30-35 days old plant. Maintain adequate moisture for longer period to permit spore germination
with the help of sprayer. Disease can also be created by spraying the spore suspension prepared from the pure culture of fungi or placing a pinch of leaf meal
(prepared by grinding dried diseased leaves collected from the previous season) into whorl of each plant at 30-35 centimeter plant height with spray of 10-12 ml of water in whorl in case of dry weather. Second inoculation can be followed if the
symptoms do not appear even after a week of first inoculation. Data can be recorded on 30-35 days after inoculation following rating scale of Payak and
Sharma# (1983) mentioned below:
Rating
scale
Disease severity (%) PDI* Disease reaction
1.0
Very slight to slight infection, one or
two to few scattered lesions on lower leaves
20.0 Resistant
(Score: ≤ 2.0) (PDI: ≤ 40.0)
2.0 Light infection, moderate number of lesions on lower leaves only
40.0
3.0 Moderate infection, abundant lesions on lower leaves, few on middle
leaves
60.0 Moderately resistant (Score: 2.1 – 3.0)
(PDI: 40.1 – 60.0)
4.0
Heavy infections abundant on lower
and middle leaves, extending to upper leaves
80.0
Moderately susceptible
(Score: 3.1 – 4.0) (PDI: 60.1 – 80.0)
5.0 Very heavy infection, lesions abundant on almost all leaves plants prematurely dry or killed by the
disease.
100.0 Susceptible (Score: ≥4.1) (PDI: ≥ 80.0)
*Percent disease index (PDI)
2. Banded leaf and sheath blight (BLSB)
Soak barley grains in water for 24 hours and dispense 40g in 250 ml
Erlenmeyer flask after removing excess water; autoclave at a pressure of 1.05 kg/sq. cm for 30 minutes. Homogenize 2-3 days old growth of pathogen taken from potato dextrose agar in sterile water and seed 5 ml in each flask. Incubate at 27°C
for 10 days. Inoculations should be made during the rainy season on 30-45 days old plants with grain culture (2-4 grains) inserted between stalk and sheath at
75
second or third level from soil. Grains placed at junction of sheath and leaf can also create optimum disease level and do not fall away with strong wind or heavy rain.
Disease is recorded 45 days after inoculation on basis of following rating scale of Payak and Sharma#(1983).
Rating scale
Disease severity (%) PDI* Disease reaction
1.0 Disease on one leaf sheath only; few small, non-coalescent lesions present
20.0 Resistant
(Score: ≤ 2.0) (PDI ≤ 40.0)
1.5 Disease on two sheaths: lesions large and coalescent
30.0
2.0 Disease up to four sheaths; lesions many and always coalescent
40.0
2.5 As in disease rating symptoms of 2.0 + rind discolored with small lesions
50.0 Moderately resistant
(Score: 2.1 - 3.0) (PDI 40.1 - 60.0) 3.0
Disease on all sheaths except two internodes below the ear
60.0
3.5 Disease up to one internode below ear shoot; rind discoloration on many internodes with large depressed lesions
70.0 Moderately susceptible
(Score: 3.1 - 4.0) (PDI 60.1 - 80.0)
4.0 Disease up to the internode bearing the ear shoot but shank not affected
80.0
4.5
Disease on the ear; husk leaves show bleaching, bands and caking among
themselves as also silk fibres; abundant fungal growth between and on kernels;
kernel formation normal except being lusterless; ear size less than normal; some plants prematurely dead
90.0
Susceptible (Score: ≥ 4.0) (≥ 80.0)
5.0 In addition to disease rating symptoms of 4.5, shrinkage of stalk; reduced ear
dimensions, wet rot and disorganization of ear; kernel formation absent or
rudimentary; prematurely dead plants common; abundant sclerotial production on husk leaves, kernels, ear tips and
stalk fibres
100.0
*Percent disease index (PDI)
3. Brown stripe downy mildew (BSDM)
Artificial epiphytotic conditions can be created by placing the powdered
infected maize leaves containing spores collected during the last season containing oospores in furrows just before planting. This inoculum could also be prepared by collecting infected leaves supposed to be full of oospores from early plantings of
maize of the same season, drying leaves and making powder out of the debris. Inoculum should be placed in furrows in such a manner that seeds were in
proximity of inoculum.
76
Artificial epiphytotic condition could also be created by putting 2-3 cm pieces of freshly infected leaves containing sporangia of the fungus in the whorls of
seedlings. This should be done during cloudy weather in the evening between 5 and 7 P.M. at 17, 24 and 30 days after planting. In experimental plots, where disease
occurs year after year, only this method is adequate for creating epidemics. In areas of low disease incidence, both the methods of inoculation can be combined to obtain better results. Disease rating of individual maize varieties can be done by
evaluation all plants of the row (s) using 1-5 rating scale of Payak and Sharma# (1983) as described below:
Rating
scale
Disease severity (%) PDI* Disease reaction
1.0 Very slight infection, one or two to few
scattered lesions on lower leaves 20.0
Resistant (Score: ≤ 2.0) (PDI: ≤ 40.0)
2.0 Light infection, a few scattered to
moderate number of stripes on lower leaves
40.0
3.0 Moderate infection, abundant stripes on
lower leaves and few on middle leaves 60.0
Moderately resistant (Score: 2.1 - 3.0) (PDI: 40.1 - 60 .0)
4.0 Heavy infection, stripes abundant on lower and middle leaves extending to
upper leaves
80.0 Moderately susceptible (Score: 3.1 - 4.0)
(PDI: 60.1 - 80 .0)
5.0
Very heavy infection, stripes abundant
on all leaves. No cob formation. Plants may be killed prematurely.
100.0
Susceptible
(Score: ≥ 4.0) (PDI: ≥ 80 .0)
*Percent disease index (PDI) 4. Curvularia leaf spot (CLS)
Mass multiplication of culture is done on half cooked sorghum grains and after evaporating excess moisture from surface, the grains are filled in 500 ml conical flasks and plugged properly. These are autoclaved for two hours at 15 lbs
pressure and inoculated when cooled down at room temperature with pure culture of Curvularia lunata. After completion of mycelial growth which may take 15-20
days at temperature around 25-27 degree C, these grains are washed in RO water to get conidial suspension of 5X104 conidia per ml. A bucket full of suspension is enough for spray inoculation of two 480 meter strip. The washed grains are spread
in a tray to get again mass of conidia. After two days gap, one more spray inoculation is done as per previous method, but this time conidial suspension should
be half of the previous one.
At least three observations are made and third observation at 80-85 DAS would be final based on leaf area covered by spots caused by pathogen.
Observations are recorded using 1-5 rating scale as described below:
Rating
scale
Disease severity (%) Disease reaction
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1.0 1-20 % area of leaf infected Resistant
(Score: ≤ 2.0) (Severity: ≤ 40.0) 2.0 21-40 % area of leaf infected
3.0 41-60 % area of leaf infected Moderately resistant (Score: 2.1 - 3.0) (Severity: 40.1 - 60 .0)
4.0 61-80 % area of leaf infected Moderately susceptible (Score: 3.1 - 4.0)
(Severity: 60.1 - 80 .0)
5.0 81-100 % area of leaf infected
Susceptible
(Score: ≥ 4.0) (Severity: ≥ 80 .0)
5. Common rust (C. rust) and Polysora rust (P. rust)
The rust is an obligate parasite and thus, it is very difficult to grow it on artificial media under laboratory condition. Though, for some specific purposes
small amount of inoculum can be grown under laboratory condition on detached leaf culture. But, this meager amount of culture obtained by such method is not
sufficient to be utilized for large scale screening trials under field conditions. Therefore, naturally infected leaves showing large number of uredopustules may be collected from different places so that all the prevalent races in the areas may be
utilized for screening the materials against the prevalent rust fungus.
The infected leaves thus collected should be macerated thoroughly in
between two palms of the hands dipped under a bucket of water until the water gets sufficiently coloured. The uredospores can also be collected on a butter paper by tapping the severely infected leaves with fingers and then stored in glass vial or
glass tube which can be sealed easily under a flame. The uredospores, thus obtained may be kept for longer period in the freezer at lower temperature i.e.
5-7°C and can also be easily carried to some distant places for inoculation purposes.
For inoculating the plants in a field use of a knapsack sprayer is very useful.
The spore suspension should be sprayed over the plants during the second half of the day when the sun becomes mild. While spraying inoculum, the nozzle of the
sprayer should be kept over whorl of the plant and all the leaves may be sprayed thoroughly. The spore suspension must be stirred continuously during spraying as the light spores aggregate together on the upper surface of the water.
Repeating the inoculation two to three times gives a good result. In addition 2-4 lines of susceptible varieties grown as border rows around the screening plots
also help to spread the disease. Disease rating is done as per scale devised by Payak and Sharma# (1983).
Rating scale
Disease severity (%) PDI* Disease reaction
1.0 Very slight to slight infection, one or two 20.0 Resistant
78
Rating
scale
Disease severity (%) PDI* Disease reaction
to few scattered pustules on lower
leaves only.
(Score: ≤ 1.0)
(PDI: ≤ 20.0)
2.0 Moderate number of pustules on lower leaves only (light infection)
40.0
Moderately resistant
(Score: 1.1 - 2.0)
(PDI: 20.1 - 40.0)
3.0 Abundant pustules on lower leaves; few
on middle leaves (moderate infection) 60.0
Moderately susceptible
(Score: 2.1 - 3.0)
(PDI: 40.1 - 60.0)
4.0 Abundant pustules on lower and middle leaves; extending to upper leaves
(heavy infection)
80.0
Susceptible
(Score: 3.1 - 4.0)
(PDI: 60.1 - 80.0)
5.0
Abundant pustules on all leaves, plant
may dry prematurely or killed by the disease (very heavy infection)
100.0
Highly susceptible
(Score: ≥ 4.0)
(PDI: ≥ 80.0)
*Percent disease index (PDI)
6. Sorghum downy mildew(SDM)
Screening through direct inoculation with conidia:
Collection and maintenance of inoculum: Sorghum plants showing systemic infection of downy mildew from the farmer’s fields in and are collected during morning hours, preserved in polythene bags and brought to the laboratory. Conidiophores and conidia
from the white bloom found on the lower surface of the leaves are washed with a fine jet of distilled water and conidial suspension is collected from the sorghum leaves. The
seedlings of susceptible cultivar are spray inoculated at 2 leaf stage (6-7 days old) with the conidial suspension collected from the sorghum leaves. The inoculation of the
seedlings is continued till the plants reached 15 days and systemic symptoms are seen. The inoculum from these plants is multiplied by spray inoculating to the fortnightly sowings of maize. The infected plants are maintained in the plot
throughout the experimental period. Artificial inoculation technique developed by Lal and Singh (1984) is followed to induce the disease incidence by spraying conidial
suspension between 2.30 a.m. and 4.00 a.m. Evaluation of maize genotypes under artificial inoculation: Maize genotypes are
evaluated against sorghum downy mildew by artificial inoculation. Artificial
inoculation is done when the plants are at two leaves stage as described by Lal and Singh (1984). Diseased plants from which inoculum required to be drawn is
sprayed with water at 6.00 PM so that leaves would have a thin film of water for
79
good sporulation. By 2.00 AM, the inoculation crew assembles in the field with cleaned sprayers, torches and buckets. By 2.30 AM the diseased leaves with
good sporulation are searched and washed in the water at the rate of 15 leaves per litre of water collected in the buckets. This operation is completed by 3.00
AM. Then the collected spore suspension in different buckets is thoroughly mixed and made upto 25 litres. The 25 litres of conidial inoculum is collected from 375 diseased leaves. The inoculation is completed by 4.00 AM with hand
compression sprayer. Between 6.00 AM and at 6.00 PM water spray is given to the inoculated plot to create the required humidity artificially. With this method
100 percent disease incidence was created. Spreader row technique: Spreader rows are sown 15-20 days prior to the
sowing of the entries in 2.5 meter bands with a row spacing of 60 cm and plant
to plant spacing of 30 cm. each band consisting of four rows surrounding on all the four directions. For this, highly susceptible variety will be used. Inoculation
of these spreader rows is done by following the above artificial inoculation procedure. Test entries were sown as mentioned above.
Per cent disease incidence is recorded 35 days after sowing and the entries are
classified according to their disease reaction as described by Lal and Singh (1984).
Disease incidence (%) Disease reaction
≤ 10 Resistant
10.1 – 25.0 Moderately resistant
25.1 – 50.0 Moderately susceptible
≥ 50.0 Susceptible
7. Rajasthan Downy Mildew (RDM)
Downy mildew nurseryis required for artificial inoculation purposes.
Susceptible maize cultivar is grown in cage house and the plants are inoculated at seedling stage by placing bits of downy mildew infected grasses Heteropogoncontortus and H. melanocarpus. Humidity around 90% is maintained in
the cage house. Chlorotic symptoms along with light green color extends up to upper green portion are typical symptoms. During midnight hours a layer of conidia
can be seen. These plants serve as source of inoculum for artificial inoculation.
Since the pathogenis of nocturnal nature and produces conidia during 12:00 to 6 AM, hence the freshly harvested conidia are collected in distilled water or RO
water. Before collecting conidia the leaves can be washed before an hour so as to get fresh viable conidia. For screening the test entries, susceptible entries should be
planted before 15 days and should be inoculated first. Since this pathogen does not form oospores on maize, hence sick plot technique does not work. The conidial suspension of harvested conidia is filled in dropping bottle to put drops of inoculum
at seedling stage (6-7 days old) in the whorl (a cup like structure of upper leaf) during 3-5 AM. This should be done for 4-5 days regularly to avoid any escape.
After 15-20 days symptoms become visible.
The observation is recorded as percent infected plants in a row out of total plants. At least three observations are taken at 30, 50 and 80 DAS. The last
80
observation is considered as final, but number of plants is considered as of first observation. This is because some plants die and disappear due to infection. The
entries are classified according to their disease reaction as described by Lal and Singh (1984) for SDM.
8. Post flowering stalk rots (Charcoal rot, Fusarium stalk rot and Late wilt)
Screening for resistance against these diseases can is easily be donein sick plots. However, artificial inoculation is necessary where such plots are not available.
For this purpose the fungal material should be isolated from the infected stalks, cultured and multiplied in the laboratory as described below.
Small bits cut from the infected stalks should be surface sterilized with 0.1
per cent mercuric chloride solution for one minute followed by washing in sterile distilled water. Finally a single bit is to be aseptically transferred to sterilized potato
dextrose agar days at 26±2°C for getting the fungal hyphae to come out from the infected bits. Finally, the fungal hyphae is to be aseptically transferred to culture tubes containing the sterile PDA medium and to be incubated for about 10 days to
get the stock culture of the pathogen to be used for increase of the inoculum in the laboratory for field inoculation.
Among various methods of field inoculation, the toothpick inoculation is followed for these diseases under the co-ordinatedprogrammes. Round bamboo
toothpicks about 6.5 cm long are boiled three times (about 1 hour each time) in tap water to remove toxic substances. After each boiling these are thoroughly washed in fresh water and dried in the sun. When these are thoroughly dry, they are
loosely packed in bundles and put into the glass jars/ bottles and enough potato dextrose broth (one- third length of toothpicks) is added to thoroughly moisten the
toothpicks plus some quantity in the bottom of the jars. The jars with the toothpicks are autoclaved immediately after the broth is added. Later the sterilized toothpicks are inoculated with the culture of the pathogen aseptically. The growth
of the fungus covers the toothpicks and inoculum is ready for use in about 10 days.
Inoculations should be made just after flowering stage of plants. For
inoculating plants, the lower internode (second/third) above soil level is opened with a jabber and the toothpick is inserted into the hole. The jabber is made by driving a nail of the diameter of the toothpick into a wooden handle. The head of
the nail is ground off to a point and to the desired length (2cm). The round toothpicks effectively seal the hole in the stalk and prevent drying. The
measurement is based on the proportion of disease present in the inoculated internodes and its subsequent spread. For scoring disease severity of PFSR, 1-9 rating scale of Payak and Sharma# (1983) is followed:
Rating scale
Disease severity (%) PDI* Disease reaction
1.0 Healthy or trace/slight discolouration at the site of inoculation.
11.11 Resistant
81
2.0 Up to 50% of the inoculated internode is
discoloured 22.22
(Score: ≤ 3.0)
(PDI: ≤33.33)
3.0 51-75% of the inoculated internode is
discoloured 33.33
4.0 76-100% of the inoculated internode is
discoloured 44.44 Moderately resistant
(Score: 3.1- 5.0)
(PDI: 33.34 - 55.55) 5.0 Less than 50% discolouration of the
adjacent internode 55.55
6.0 More than 50% discolouration of the adjacent internode
66.66 Moderately susceptible
(Score: 5.1 - 7.0)
(PDI: 55.56 - 77.77) 7.0 Discolouration of three internodes 77.77
8.0 Discolouration of four internodes 88.88 Susceptible
(Score: ≥ 7.0)
(PDI: ≥ 77.77) 9.0
Discolouration of five or more internodes and premature death of plant
99.99
*Percent disease index (PDI)
9. Bacterial stalk rot (Pre-flowering stalk rot)
A virulent isolate of Erwinachrysanthemi corn pathotype should be selected for inoculation. To maintain the virulence of the bacterium, it should be inoculated on healthy plants and then reisolated every year before mass inoculation. In order to
isolate a virulent strain, the inoculated plants showing characteristic symptoms of the disease are selected. A small piece of rotten internode is immediately dipped
into mercuric chloride solution (1:1000) for 5 seconds and passed through three changes of sterile water. The piece is then cut into two halves with sterilized blade, put into little sterile water and then teased apart with sterile needle. The small
quantities of resulting suspension are then removed with a flamed wireloop and streaked out on well dried nutrient agar plates, the aim being to separate the cells
so that they produce individual colonies. The characteristic colonies are identified after 2 days of incubation at 30°C and used for subculturing. The culture is used for
testing the pathogenicity. The cultures which induce the typical symptoms of the disease within 48 hours of inoculation are used for mass inoculation. The inoculum is increased for mass inoculation on nutrient broth for 48 hours at 30°C. The
inoculum was diluted 10 times with sterile water to maintain a concentration of approximate 1X107-9 bacteria/ml.
The inoculation may be carried out when the crop is at the pre-silking stage or until flowering has reached 75%. To inoculate the plants a diagonal hole is made in the middle of second internode from the ground to the pith. One milliliter of
bacterial suspension is injected into the plant through the hole by a hypodermic syringe. If necessary, a second inoculation may be done one week later in the third
internode from the ground. Percent disease incidence is recorded 15 days after sowing and the entries are classified according to their disease reaction as described by Lal and Singh (1984) for SDM.
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10. Maize cyst nematode (HeteroderaZeae)
Plant parasitic nematodes are responsible to causes 10.2% losses o maize.
Though, large number of plant parasitic nematodes attacks on maize but maize cyst nematode (Heteroderazeae) is considered as most important and therefore,
screeningtrials are carried out under artificially inoculated conditions in permanent plots to find out source of resistance against maize cyst nematode (Heteroderazeae). The observations on nematode infestation are recorded after 45 days of germination.
The varieties/hybrids/ lines are categorized on the basis of cyst/plant as mentioned below:
S. No. Number of cyst/plant Category
1 0 - 4 cyst/plant Resistant
2 Above 4 - 9 cyst/plant Moderately Resistant
3 Above 9 cyst/plant Susceptible
* Calculation of Percent Disease Index (PDI) of Foliar Diseases of Maize
Percent disease index (PDI) is calculated using the following formula of Mckinney
(1923).
Sum of individual rating 100
Percent disease index (PDI)= X No. of leaves examined Maximum disease rating
On the basis of PDI, the inbred lines/ varieties/ hybrids can be classified as resistant (R), moderately resistant (MR), moderately susceptible (MS) and
susceptible (S). The test inbred lines/ varieties/ hybrids with resistant reaction are considered acceptable for a breeding programme whereas test inbred lines/ varieties/ hybrids with moderately resistant are acceptable when lines with resistant
reaction are not available.
# M.M. Payak and R.C. Sharma. Disease rating scales in maize in India.
In:Techniques of Scoring for Resistance to Important Diseases of Maize. All India Coordinated Maize Improvement Project, Indian Agriculture Research Institute, New Delhi, 1983, pp. 1-4.
83
Entomology: Technical Programme for 2015-16
Dr. Pradyumn Kumar PI Entomology presented the plan of work for Kharif-2015,
Spring-2016 and Rabi-2015. Five trials will be carried out in Entomolgy programme
at Delhi, Dholi, Hyderabad, Karnal, Kolhapur, Ludhiana and Udaipur centres during
kharif 2015.
Three trials will be carried out in Hyderabad and Kolhapur centres during rabi 2015.
Trial on screening inbred lines against shoot fly will be carried out at Delhi, Karnal
and Ludhiana centres during spring 2016.
Kharif
ET 1: Evaluation of maize AICRP trials entries against Chilo partellus under artificial infestation for AVT I and II
ET 2 : Evaluation of inbred lines against Chilo partellus under artificial infestation
(30 lines + 2 checks, 2 replications)
ET 3: Monitoring of Helicoverpa armigera by pheromone traps ET 4: Parasitization level of biocontrol agents in maize ecosystem
ET 5 : Evaluation of insecticides against C. partellus (3rd Year)
Insecticide Dose
Chlorantaniliprole 20 SC 0.3 ml/lit
Chlorantaniliprole 20 SC 0.4 ml/lit
Flubendiamide480 SC 0.1 ml/lit
Flubendiamide480 SC 0.2 ml/lit
Novaluron 10 EC 0.75ml/lit
Novaluron 10 EC 1 ml/lit
Deltamethrin 2.8 EC 0.4 ml/lit
Deltamethrin 2.8 EC 0.8 ml/lit
Untreated Control
Rabi
ET 6: Evaluation of maize AICRP Trials entries against Chilo partellus and S. inferens under artificial infestation for AVT I and II (Kolhapur & Hyderabad)
ET 7: Evaluation of inbred lines against Chilo partellus and S. inferens under artificial infestation (Kolhapur & Hyderabad) ( 30 lines + 2 checks, 2
replications) ET 8: LIR vs yield relationship for S. inferens. (Hyderabad, Karnal, Ludhiana
and Delhi) (2nd year)
Spring
ET 9: Evaluation of inbred lines against Sorghum shoot fly under natural infestation (Delhi, Karnal and Ludhiana) ) ( 30 lines + 2 checks, 2 replications)
ET 10: Monitoring of Helicoverpa armigera by pheromone traps (Ludhiana)
84
Session XII
Plenary Session
Dr. O.P. Yadav, Director, IIMR presented the recommendations of VIC Meeting. He
summarized that out of 18 proposals of maize hybrids/OPVs received from various
institutions for variety identification seven proposals were accepted and identified
for release.
The salient recommendations under Breeding section was presented which
includes (i) The number of test entries were restricted to 2 in each maturity group
per centre / research institutions / organizations; (ii) The per cent superiority over
best performing zone specific check was raised to 10% under later maturity to
promote entries from IVT to AVT-I.
The approved breeding work-plan for kharif-2015, Rabi 2015-16 as well as trials
proposed and accepted under ICAR-CIMMYT research work plan 2015-16 was also
presented.
Dr. Ashok Kumar, presented the recommendations emerged from Crop
Production group were as follows:
A total of nine ongoing and two new trials will be part of work plan for 2015-16
in crop production (Action: PI and all agronomist) In tillage experiments, soil must be sampled from three depths (0-5, 5-15 and
15-30 cm) for soil physical and chemical properties after three years of
experimentation. (Action: Centre concerned) The BISA, Ludhiana trial on water management in maize will be part of AICRP
maize. (Action: Ludhiana centre) The weather data on daily basis must be supplied with crop data. (Action: All
AICRP centers)
Publications should be made from the experiments completed three cycles. (Action: PI Agronomy and AICRP centers)
Herbicides molecules will be arranged by PI Agronomy for weed management trial. (Action: PI Agronomy)
Under rain fed conditions residue retention @ 5 t/ha on the soil surface is
recommended for enhancing maize productivity by 9-29%.
The intercropping of soybean/black gram in maize grown during kharif season is
recommended for Zone I, III and V.
Pathology and Nematology
1. At Coimbatore centre, adequate disease pressure of SDM disease is not being
created owing to unfavorable condition since last 2-3 years and there is need
Chairman Co-Chairman Members
Dr. B. S. Dhillon, Vice-chancellor,
PAU, Ludhiana
Dr. S. K. Vasal, Eminent
Maize Scientist
Dr. I.S. Solanki, ADG (FFC), ICAR
Dr. O.P. Yadav, Director, IIMR
85
to identify the best favorable conditions for it during the year. Charcoal rot is another emerging disease since last few years in Tamil Nadu and therefore,
screening against it should be initiated from this very kharif 2015 season. (Action: Coimbatore Centre)
2. The centre(s) already conducting disease survey at station level should send the survey report to IIMR to know disease scenario of that particular region.
(Action: Godhra centre)
3. The identified resistant inbreds from respective centres should be maintained and performance should be checked against important disease(s) of that
particular region. (Action: All centres)
4. Data of concluded experiments on yield losses should be jointly published
with prior permission of Director, IIMR. (Actions: PFSR– Dr. Meena Shekhar; BLSB- Dr. Robin Gogoi; TLB- Dr.
Rakesh Devlash, SDM– Dr. Mallikarjun)
5. Yield loss trials need to be conducted for two or more years.
(Action: Ludhiana (MLB, charcoal rot), Mandya (SDM, Polysora rust), Arbhavi (common rust), Udaipur (RDM), Bajaura (TLB)
6. Seed material should reach all locations of Zone I before 20th of June. (Action: IIMR)
7. Susceptible checks have to be included at every 10th /20th row in all disease
screening trials as per availability of land and seed of same is to be maintained at the centre.
(Action: All centres)
8. Very few centres has initiated development of sick plot for screening against soil borne disease(s) and therefore, the same should be taken up on priority in
this season. Once developed, inoculum load in sick plot should be calculated while conducting trials. Director of Research/ Director of centres will ensure
this and send compliance report to IIMR. (Action: All concerned centres)
9. Newly joined scientists need exposure training in disease screening techniques and rating scale.
(Action: Dholi, Karnal, Medinapur centres)
10.Number of resistant lines made available to breeder(s) for their utilization in breeding program need to be provided in next workshop.
(Action: All concerned centres)
11.The group made thorough discussion over revision of rating scales from 1-5 to
1-9. The PI provided the scales with severity charts for assessing the foliar diseases and invited feedback in next workshop for further follow up.
(Action: All centres)
12.Studies on ecofriendly management of cyst nematode (H. zea) through cultural practices and nematophagous fungi need to be strengthened.
(Action: Udaipur centre)
86
13.Disease screening against RDM and FSR can be conducted at Banswara centre of MPUAT, Udaipur.
(Action: IIMR) 14.As decided last year registration of resistant line(s) should be taken up by
centres. (Action: All concerned centres)
15.All the survey data should be correlated with meteorological parameters. (Action: All concerned centres)
Entomology
Dr. J.C. Sekhar, presented the recommendations emerged from Entomology
group were as follows:
INGR14014, an early maturity inbred line resistant to Pink borer, Sesamia
inferens may be used as resistant check in screening coordinated rabi trials
and the same is available for breeding programme
Two inbred lines namely, DMR E 63 and WNZEXOTICPOOL1A have been
found moderately resistant to Chilo partellus (after three years of testing)
and these are available for breeding programme
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