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Introduction to Introduction to Liquid Liquid
ChromatographyChromatographyColumnsColumns
System ComponentsSystem Components
ApplicationsApplications
TroubleshootingTroubleshooting
Susan M. Steinike, M.S HPLC Marketing Department February, 2006
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Modern HPLCModern HPLC
Late 1970s/early 1980sLate 1970s/early 1980s Instrumentation developed for high pressure Instrumentation developed for high pressure
solvent delivery: pumps, autosamplers, solvent delivery: pumps, autosamplers, diode array detectorsdiode array detectors
More uniform packing material produced More uniform packing material produced for columnsfor columns
Last 20 yearsLast 20 years Nothing really “new”, but by returning to Nothing really “new”, but by returning to
the basic theory of chromatography, even the basic theory of chromatography, even better columns are on the market: smaller better columns are on the market: smaller particle sizes which yield faster separations, particle sizes which yield faster separations, but require hardware to withstand higher but require hardware to withstand higher pressures.pressures.
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What is What is Chromatography?Chromatography?
Separation of a mixture into individual Separation of a mixture into individual components.components.
The separation uses a Column The separation uses a Column (stationary phase) and Solvent (mobile (stationary phase) and Solvent (mobile phase).phase).
The components are separated from each The components are separated from each other based on differences in affinity for other based on differences in affinity for the mobile or stationary phase.the mobile or stationary phase.
The goal of the separation is to have the The goal of the separation is to have the best RESOLUTION possible between best RESOLUTION possible between components.components.
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The Most Basic Explanation The Most Basic Explanation of Chromatography Everof Chromatography Ever
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How Do You Get How Do You Get Separation?Separation?
Hardware: pumps, injector, detectorHardware: pumps, injector, detector
Column: particle diameter, column Column: particle diameter, column size, packing materialssize, packing materials
Our seminar will focus on the Our seminar will focus on the contribution of each factor to contribution of each factor to perform separations.perform separations.
, and the dreaded , and the dreaded equationsequations
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HPLC and Pre-HPLC HPLC and Pre-HPLC TechniquesTechniques
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Column TypesColumn Types Reversed-Phase LCReversed-Phase LC
Nonpolar stationary phase: C8, C18Nonpolar stationary phase: C8, C18 Polar mobile phase: Water, ACN, MethanolPolar mobile phase: Water, ACN, Methanol The MOST polar compound comes out firstThe MOST polar compound comes out first
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Reversed Phase HPLC Reversed Phase HPLC ColumnsColumns
C-18, C-8:C-18, C-8: Rugged, general purpose, highly Rugged, general purpose, highly retentiveretentive
C-3, C-4:C-3, C-4: Less retentive, used mostly for Less retentive, used mostly for peptides peptides & proteins & proteins
Phenyl:Phenyl: Greater selectivity than alkyl-bonded Greater selectivity than alkyl-bonded Cyano:Cyano: Moderate retention, normal & rev. Moderate retention, normal & rev.
phasephase Amino:Amino: Weak retention, good for Weak retention, good for
carbohydratescarbohydrates
The cyano column with a high polarity mobile phase (Water/MeOH) will act as a reversed phase column.
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Typical Column SizesTypical Column Sizes
Particle size: 5 µm, 3 µm, and smallerParticle size: 5 µm, 3 µm, and smaller Monodispersed means particles are the same Monodispersed means particles are the same
sizesize Very important for stable pressure and flowVery important for stable pressure and flow Smaller particles produce higher system Smaller particles produce higher system
pressurepressure Pore size: 100-120 A is typicalPore size: 100-120 A is typical Surface area: 300-350 mSurface area: 300-350 m22/g/g Carbon load: 9-12% for C8, 16-20% for Carbon load: 9-12% for C8, 16-20% for
C18C18 Higher carbon load = better resolution but Higher carbon load = better resolution but
longer run timeslonger run times Lower carbon load = shorter run times, but Lower carbon load = shorter run times, but
may change selectivity vs. higher carbon loadmay change selectivity vs. higher carbon load
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Idealized HPLC Idealized HPLC SeparationSeparation
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Capacity Factor, kCapacity Factor, k’’
The relative degree to which an analyte component is delayed as it is eluted through a given system (retentivity).
k’ = (Vr - V0)/V0 = (tr - t0)/t0
Where Vr = peak retention volume; V0 = column void volumetr = peak retention time; t0 = peak void time
The larger the kThe larger the k’’, the later the analyte elutes after the void., the later the analyte elutes after the void.
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Mobile Phase Strength Mobile Phase Strength vs. kvs. k’’
4.6 mm ID Column, 1 mL/min, Changing MeOH % vs Water
100%
90%
80%
70%
60%
50%
0.079
0.212
0.472
1.127
2.813
Capacity Factor for Butyl Paraben (Peak 4)
7.666
100%
90%
80%
70%
60%
50%
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Temperature Effect on kTemperature Effect on k’’
20°C
25°C
30°C
35°C
40°C
45°C
50°C
2.1 mm ID Column, 0.35 mL/min, 50/50 MeOH/Water
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Questions About Questions About Columns?Columns?
Next – HPLC Next – HPLC System System
ComponentsComponents
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HPLC Pumps – 2 Basic HPLC Pumps – 2 Basic TypesTypes
Dual PistonDual Piston Two pistons with equal volume (10 µL Two pistons with equal volume (10 µL
each)each) During each stroke, 10 µLDuring each stroke, 10 µL is deliveredis delivered Best for low flow rates (< 1 mL/min)Best for low flow rates (< 1 mL/min) Little to NO pulsation, so it’s ideal for Little to NO pulsation, so it’s ideal for
pulse sensitive detectors like RID and pulse sensitive detectors like RID and CDDCDD
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Dual Piston PumpDual Piston Pump
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Sample Injection – Sample Injection – Automatic Automatic
Needle-in-the-flowpath autosamplerNeedle-in-the-flowpath autosampler Sample loop and needle are a single piece of Sample loop and needle are a single piece of
tubingtubing Loop and needle are cleaned during the runLoop and needle are cleaned during the run Metering pump draws sample very preciselyMetering pump draws sample very precisely
Advantages: no sample loss, low Advantages: no sample loss, low carryovercarryover
Disadvantages: higher cost, more delay Disadvantages: higher cost, more delay volume for gradientvolume for gradient
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Sample Injection to Flow Sample Injection to Flow PathPath
Sample LoadingSample Loading
Sample Sample Injection – Injection – Everything Everything drawn into the drawn into the needle goes to needle goes to the column.the column.
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HPLC Column OvensHPLC Column Ovens
Block heater with solvent preheaterBlock heater with solvent preheater Column is housed between 2 metal platesColumn is housed between 2 metal plates Mobile phase is plumbed into the block for Mobile phase is plumbed into the block for
preheatingpreheating
Forced airForced air Column is in a large chamber with air Column is in a large chamber with air
circulationcirculation Better temperature equilibrationBetter temperature equilibration Room for column switching valvesRoom for column switching valves
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Why Use a Column Oven?Why Use a Column Oven?
Retention times decrease, and Retention times decrease, and higher flow rates are possiblehigher flow rates are possible
20°C
25°C
30°C
35°C
40°C
45°C
50°C
2.1 mm ID Column, 0.35 mL/min, 50/50 MeOH/Water
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HPLC Detectors – UV-VIS HPLC Detectors – UV-VIS
UV-VISUV-VIS Wavelength range 190-700 nmWavelength range 190-700 nm D2 and W lampsD2 and W lamps
Most common HPLC detector for a variety Most common HPLC detector for a variety of samplesof samples Proteins and peptidesProteins and peptides Organic moleculesOrganic molecules PharmaceuticalsPharmaceuticals
Monitor 2 wavelengths at one timeMonitor 2 wavelengths at one time
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HPLC Detectors – UV-VISHPLC Detectors – UV-VIS
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HPLC DetectorsHPLC Detectors
Refractive IndexRefractive Index For samples with little or no UV For samples with little or no UV
AbsorptionAbsorption Alcohols, sugars, saccharides, fatty acids, Alcohols, sugars, saccharides, fatty acids,
polymerspolymers Best results when RI of samples is very Best results when RI of samples is very
different from RI of mobile phasedifferent from RI of mobile phase Flow cell is temperature controlled with Flow cell is temperature controlled with
a double insulated heating block.a double insulated heating block.
REQUIRES isocratic separationsREQUIRES isocratic separations REQUIRES low pulsation pumpsREQUIRES low pulsation pumps
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HPLC Detectors – RI HPLC Detectors – RI BalanceBalance
Fill sample and Fill sample and reference cell with reference cell with mobile phase.mobile phase.
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HPLC Detectors – RI HPLC Detectors – RI AnalyzeAnalyze
Mobile phase flows Mobile phase flows through sample through sample side only.side only.
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HPLC Detectors – RI HPLC Detectors – RI AnalyzeAnalyze
As the refractive index changes, the image on the As the refractive index changes, the image on the photodiode is deflected or “unbalanced”, and the photodiode is deflected or “unbalanced”, and the difference in current to the photodiode is measured.difference in current to the photodiode is measured.
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HPLC System TypesHPLC System Types
Isocratic systemIsocratic system Same mobile phase concentration Same mobile phase concentration
throughout the separationthroughout the separation Use 1 pump and pre-mix solventsUse 1 pump and pre-mix solvents Use 1 pump and a valve for 4 different Use 1 pump and a valve for 4 different
solventssolvents Use 2 pumps and vary the amount Use 2 pumps and vary the amount
coming from each pumpcoming from each pump
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Isocratic SeparationIsocratic Separation
1 pump and premixing1 pump and premixing 4.6 mm ID Column, 1 mL/min, Changing MeOH
% vs Water
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Isocratic SeparationIsocratic Separation
1 pump with valve and premixing1 pump with valve and premixing
To Column
A B C D
A = 80% Methanol, 20% Water
B = 70% Methanol, 30% Water
C = 60% Methanol, 40% Water
D = 50% Methanol, 50% Water
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Isocratic SeparationIsocratic Separation
1 pump with mixer – let the pump do the work!1 pump with mixer – let the pump do the work!
Method 1: A.CONC = 20%, B.CONC = 80%
Method 2: A.CONC = 30%, B.CONC = 70%
Method 3: A.CONC = 40%, B.CONC = 60%
Method 4: A.CONC = 50%, B.CONC = 50%
To Column
A B C D
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Low Pressure GradientLow Pressure Gradient
1 Pump, solvents are mixed before 1 Pump, solvents are mixed before the pumpthe pump
REQUIRES degassingREQUIRES degassing
To Column
A B C D
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Low Pressure GradientLow Pressure Gradient
1 Pump, solvents are mixed before 1 Pump, solvents are mixed before the pumpthe pump
REQUIRES degassingREQUIRES degassing
To Column
A B C D
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Questions About System Questions About System Types?Types?
Next: Troubleshooting Next: Troubleshooting and How to Take Care of and How to Take Care of Your Column and HPLC Your Column and HPLC
SystemSystem
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HPLC TroubleshootingHPLC Troubleshooting
Pressure: too much or too littlePressure: too much or too little Leaks: pump, autosampler, detectorLeaks: pump, autosampler, detector Reproducibility: pump, autosamplerReproducibility: pump, autosampler
Column Care: Flushing and Column Care: Flushing and equilibrationequilibration
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Pump TroubleshootingPump Troubleshooting
No pressure, or fluctuating pressureNo pressure, or fluctuating pressure Pump may not be completely full of liquid – Pump may not be completely full of liquid –
check solvent inlet linecheck solvent inlet line Air in check valve – always degas mobile Air in check valve – always degas mobile
phase!phase! ““Stuck” check valve – the pump may have Stuck” check valve – the pump may have
been idle for too long and solvent has dried been idle for too long and solvent has dried inside the check valve.inside the check valve.
Poor quality solvent: may contain resins Poor quality solvent: may contain resins that coat the ball inside the check valve, that coat the ball inside the check valve, and that film won’t let the ball seat properlyand that film won’t let the ball seat properly
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Pump TroubleshootingPump Troubleshooting
High PressureHigh Pressure Outlet frit may be blocked with particles from Outlet frit may be blocked with particles from
mobile phase or seal materialmobile phase or seal material
LeaksLeaks Damage to seal and/or plunger due to several Damage to seal and/or plunger due to several
factorsfactors Misaligned plungerMisaligned plunger Solvent incompatibility with seal materialSolvent incompatibility with seal material Salt crystal buildup from buffers – use a rinse kit!Salt crystal buildup from buffers – use a rinse kit!
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Pump TroubleshootingPump Troubleshooting
Retention Time ReproducibilityRetention Time Reproducibility For a dual piston pump, only one side For a dual piston pump, only one side
may be filled with liquid – check solvent may be filled with liquid – check solvent inlet linesinlet lines
Temperature change (may not be the Temperature change (may not be the pump’s fault)pump’s fault) A 1A 1oo shift in temperature can result in a 1- shift in temperature can result in a 1-
2% shift in retention time2% shift in retention time Avoid drafty locations in the labAvoid drafty locations in the lab Use a column oven when possibleUse a column oven when possible
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Autosampler Autosampler TroubleshootingTroubleshooting
High PressureHigh Pressure Particulates from mobile phase, sample, Particulates from mobile phase, sample,
pump may be trapped in the inlet tubing pump may be trapped in the inlet tubing or valveor valve
Filter mobile phase AND sample when possibleFilter mobile phase AND sample when possible
LeaksLeaks Fittings may be loose on the valveFittings may be loose on the valve
Tighten fittings properly and don’t exceed the Tighten fittings properly and don’t exceed the pressure limit of the autosamplerpressure limit of the autosampler
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Autosampler Autosampler TroubleshootingTroubleshooting
Area % ReproducibilityArea % Reproducibility Always degas rinse phase, and use Always degas rinse phase, and use
some volume of liquid for rinsing to some volume of liquid for rinsing to keep all flow paths in the valves full of keep all flow paths in the valves full of liquidliquid
Make sure the needle stroke is deep Make sure the needle stroke is deep enough to draw sample from the vialenough to draw sample from the vial
Check for leaks on the valve fittings, Check for leaks on the valve fittings, and the connection to the column inletand the connection to the column inlet
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Detector Detector TroubleshootingTroubleshooting
Spiky BaselineSpiky Baseline Air bubble in flow cell – degas mobile phase!Air bubble in flow cell – degas mobile phase!
Put some restriction on the cell outlet, but not too Put some restriction on the cell outlet, but not too much! Tubing with 0.005” i.d. is fine.much! Tubing with 0.005” i.d. is fine.
LeaksLeaks Cracked flow cellCracked flow cell
Don’t exceed the pressure limit of the cellDon’t exceed the pressure limit of the cell Poor tubing connectionsPoor tubing connections
Use the proper fittings and tighten appropriatelyUse the proper fittings and tighten appropriately
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Column CareColumn Care
Follow MFR’s recommendations for Follow MFR’s recommendations for solvent compatibility, flow rate, and solvent compatibility, flow rate, and pressure limitspressure limits
Filter samples when possibleFilter samples when possible Particulates will build up on the inlet frit over Particulates will build up on the inlet frit over
timetime Use care when reversing column flowUse care when reversing column flow
Connect the outlet to waste, NOT inline with Connect the outlet to waste, NOT inline with the detector to prevent further contaminationthe detector to prevent further contamination
Store columns in recommended solventsStore columns in recommended solvents
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Troubleshooting Troubleshooting SummarySummary
Throw away bad parts and columns.Throw away bad parts and columns.
Leaks do not fix themselves.Leaks do not fix themselves.
If it doesn’t pass, you must degas.If it doesn’t pass, you must degas.
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