SPIFE 3000SPIFE 3000
• 11 analytes, with multiple gel sizes for every workload
• Typical throughput – up to 14 SPE and 12 IFE gels in 8 hours
• Used in hundredsof medical centers and reference laboratories worldwide
• Semi-automated electrophoresis system
• Automated sample application system
• Automated reagent application for enzymatic assays
SPIFE 3000SPIFE 3000
• Temperature-controlled electrophoresis, incubation and drying
• Automated staining and drying of visible analytes
SPIFE 3000SPIFE 3000
• Positive ID of sample tray and gel
SPIFE AssaysSPIFE Assays• Cholesterol Profile• Serum Proteins• Split-Beta SPE• Urine Proteins• Immunofixation• Urine Immunofixation• Hi Resolution Proteins• Alkaline Hemoglobins• Acid Hemoglobins• CK & LD Isoenzymes• Alk Phosphatase Isoenzymes• CSF IgG IEF• Lipoproteins
SPIFE IFESPIFE IFE
• Built-in QC wells
• Up to 210 profiles in 8 hours
• Antisera affinity & avidity balanced for highest sensitivity with least background
• 1, 2, 3, 6, 9 and 15 profile gels to handle a wide range of needs
• Acid violet stain enhances visualization
SPIFE IFE ProcedureSPIFE IFE Procedure
• Dilute automatically using the Auto Sample Handler or manually
• Load patients samples and applicator blades
• Set gel and electrodes in place in electrophoresis chamber
SPIFE IFE ProcedureSPIFE IFE Procedure
• SPIFE applies samples, allows for absorption, and begins electrophoresis
• Blot surface of the gel and begin electrophoresis
• After electrophoresis, remove buffer ridges from the gel
SPIFE IFE ProcedureSPIFE IFE Procedure
• Set Rigid Antisera Template in place
• Fast and efficient antisera application
• Pipette color-coded antisera for an entire profile at once
SPIFE IFE ProcedureSPIFE IFE Procedure
• Absorb excess antisera from channels, preventing cross-contamination
• Blot gel surface, using antisera template as press and allow SPIFE to incubate and dry the gel
SPIFE IFE ProcedureSPIFE IFE Procedure
• Move gel to SPIFE’s staining unit for automatic wash, stain, destain and dry
• Finished!All SPIFE IFE gels include integral control wells for optional use of ImmunoFix Controls to verify antisera reactivity
• 108 slides
• Basics of protein structure
• Disease states associated with protein abnormalities
• Suggested protocol for evaluating monoclonals
• When to run, how to run, and what to look for
• Patterns to interpret from simple to complex
• Covers serum and urine
New Educational CDProtein Electrophoresis and IFE
An Interpretive Guide
Auto Sample Handler• Provides positive
sample ID from tube to tray to gel
• Primary tube sampling with liquid level sensing
• Bar code reader with LIS interface
Auto Sample HandlerAuto Sample Handler• Automatically performs
dilutions for IFE
• Prepares sample trays, ready for SPIFE
• Interfaces to QuickScan 2000 for workload listing
QuickScan 2000
• Provide IFE and SPE results on a single report
• Patterns can be viewed and edited over the LAN
• 10,000 gel archive with easy retrieval
• Scan anything large or small in seconds:10-sample to 100-samplegels and L/S plates
QuickScan 2000QuickScan 2000
• Customized interpretive analysis and decision limits for fast reporting
• Simple to use – no slits or wavelengths to select
• Multi-pattern display color-coded for in/out of range and reviewed results
• Build your own decision limits and interpretive comments library
• Comments automatically added for “normals” and coded for quick retrieval
QuickScan 2000QuickScan 2000
• Choose quantitation of split beta SPE as one or two fractions
• Report formats can be totally customized
• Profile up to 100 scans
QuickScan 2000QuickScan 2000
SPIFE SystemSPIFE System
Contact your local Helena technical representative for more information or to arrange an in-lab
demonstration of the SPIFE System.
1-800-231-5663
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