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Quantitative Chemical Analysis
Seventh Edition
Chapter 25 High-Performance Liquid
Chromatography
Copyright © 2007 by W. H. Freeman and Company
Daniel C. Harris
Suggested problems: Chapter 25: 1a, 15 Chapter 26: 25, 28
EXAM FOUR – TUESDAY APRIL 20th Separation modes and HPLC
Pumps and Injection valves for HPLC Sample introduction and HPLC
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Silica surface and bonded phases Particle size and separation efficiency
Selecting the right solvent for HPLC Isocratic separation of a mixture of aromatic compounds
“A” is 25 mM KH2PO4 and “B” is acetonitrile. Column is a 25 cm x 0.46 cm ODS with 5 µm silica.
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Isocratic separation of a mixture of aromatic compounds cont’d Isocratic separation of a mixture of aromatic compounds cont’d
Gradient elution of the same mixture! Detectors for HPLC
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Quantitative Chemical Analysis
Seventh Edition
Chapter 26 Chromatographic Methods and
Capillary Electrophoresis
Copyright © 2007 by W. H. Freeman and Company
Daniel C. Harris
Ion exchange resins and ion chromatography
Gradient elution and ion exchange chromatography
Higher the atomic # of the lanthanide, the smaller its ionic radius and the more strongly it binds to the resin
Ion exchange chromatography - analysis of pond water
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Indirect detection
Mobile phase contains 1 mM sodium phthalate
Ion-pair chromatography
Addition of sodium octanesulfonate to the mobile phase. Negative sulfonate groups act as anion-exchange sites for analytes such as protonated organic bases (BH+)
Schematic of an instrument for capillary electrophoresis Laminar flow versus EOF profile
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Separation efficiency in HPLC versus CE Indirect detection of isotopes separated by CE
Detection challenges in CE Electrochemical Detection in CE
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Separation of neutral compounds by MEKC Integrated biochip for DNA analysis
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