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The Potential of Coconut Husk as
Phytoremediating Agent for Lead andCopper Contaminated Water Tested throughBrine Shrimp Toxicity Assay
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Objective
To adsorb copper and lead in water with the use of coconut husk.
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Procedure
Gathering of coconut husk
Weighing 10 g of coconut husk
Preparing 2 beakers with 200 ml distilled water
Preparing 2mg of lead and copper
Separately mixing lead and copper in both beakers
Serial dilution of lead and copper in six (6) respective test tubes.
Adding Brine Shrimps to both set ups. One set-up for lead and one set-up for copper. Each
set-up was duplicated one with treatment and the other without treatment.
Adding 1.0g cubed shaped coconut husk to treated test tubes.
Data collection (counting the brine shrimp mortality)
Probit Analysis
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Results:
Concentration of
Water No of Brine Shrimp
No. of Brine Shrimp
Dead Percent Kill
Log Concentration
(x)
Empirical
Probit
1 18 14 78 0 5.77
0.1 20 10 50 -1 5.00
0.01 18 8 44 -2 4.85
0.001 19 8 42 -3 4.8
0.0001 20 5 25 -4 4.33
0.00001 23 3 13 -5 3.87
Lethal Concentration of Lead Contaminated Water Without Coconut Husk
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Graphical Presentation of Log Concentration Vs. Empirical Probit Value
Mortality Analysis Lead Contaminated Water Without Coconut Husk
The values in Table 1 is plotted
and a linear regression function
is determined through algebraic
method. The function is being
determined as y = 0.3303x +5.5957 Setting the value of y at
5.0 will represent Lethal
Concentration at which 50% of
the tested organism will die.
The Lc50 of the linear
regression is -1.8035 and
extracting the antilog of x, the
researcher obtained the value
of 0.0157217 mg/L., the
concentration at which 50%
mortality of the experimental
brine shrimp is attained.
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Lethal Concentration of Lead Contaminated Water Treated With Coconut Husk
Concentration of
Water No of Brine Shrimp
No. of Brine Shrimp
Dead Percent Kill
Log Concentration
(x) Empirical Probit
1
15
10
67
0
5.44
0.1 16 7 44 -1 4.85
0.01 17 4 24 -2 4.29
0.001 14 2 14 -3 3.92
0.0001 19 2 11 -4 3.77
0.00001 15 1 7 -5 3.52
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Graphical Presentation of Log Concentration Vs. Empirical Probit Value
Mortality Analysis Lead Contaminated Water Treated With Coconut Husk
The values in Table 2 is plotted and a
linear regression function is
determined through algebraic
method. The function is beingdetermined as y = 0.3774x + 5.2419
Setting the value of y at 5.0 will
represent Lethal Concentration at
which 50% of the tested organism will
die. The Lc50 of the linear regression
is -0.64096 and extracting the antilog
of x, the researcher obtained thevalue of 0.2286 mg/L., the
concentration at which 50% mortality
of the experimental brine shrimp is
attained.
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Lethal Concentration of Copper Contaminated Water Without Coconut Husk
Concentration of
Water No of Brine Shrimp
No. of Brine Shrimp
Dead Percent Kill
Log Concentration
(x) Empirical Probit
1 21 13 62 0 5.31
0.1 25 12 48 -1 4.95
0.01 20 10 50 -2 5.00
0.001 26 9 35 -3 4.62
0.0001 25 8 32 -4 4.53
0.00001 22 4 18 -5 4.08
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Graphical Presentation of Log Concentration Vs. Empirical Probit Value
Mortality Analysis Copper Contaminated Water Treated Without Coconut Husk
The values in Table 3 is plotted and a
linear regression function is
determined through algebraic method.
The function is being determined as y
= 0.2226x + 5.3048 Setting the valueof y at 5.0 will represent Lethal
Concentration at which 50% of the
tested organism will die. The Lc50 of
the linear regression is -1.3693 and
extracting the antilog of x, the
researcher obtained the value of0.042727 mg/L., the concentration at
which 50% mortality of the
experimental brine shrimp is attained
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Lethal Concentration of Copper Contaminated Water Treated With Coconut
Husk
Concentration of
Water No of Brine Shrimp
No. of Brine Shrimp
Dead Percent Kill
Log Concentration
(x) Empirical Probit
1
11
7
64
0
5.36
0.1 13 6 46 -1 4.90
0.01 11 5 45 -2 4.87
0.001 12 2 17 -3 4.05
0.0001
12
1
8
-4
3.59
0.00001 14 1 7 -5 3.52
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Graphical Presentation of Log Concentration Vs. Empirical Probit Value
Mortality Analysis Copper Contaminated Water Treated With Coconut Husk
The values in Table 4 is plotted and a
linear regression function is determined
through algebraic method. The function is
being determined as y = 0.3986x +
5.3781 Setting the value of y at 5.0 will
represent Lethal Concentration at which
50% of the tested organism will die. The
Lc50 of the linear regression is -0.9486
and extracting the antilog of x, the
researcher obtained the value of0.11257mg/L., the concentration at which
50% mortality of the experimental brine
shrimp is attained.
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Conclusions:
Coconut husk has the potential in eliminating copper, and lead from water.
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1. That the Department of Health will investigate into this fact to confirm this result. If proven
through their effort, then publish the information to people so that they can apply this treatment
to make sure that their water is fit for drinking.
2. That people must seek medical aid if something happened to them after using this treatmentto avoid serious complications.
3. That the same study may be carried out by another researcher to find out the efficiency of
coconut husk in treating other cations from water.
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