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PROJECT
For
Structural Biology and SecondaryMetabolites
2011-2012
Submitted By: Submitted To:
Name: PRIYANSHI GIRI Dr. Pankaj.K.Mishra
Programme: Msc. Biotechnology
Email: [email protected]
Mob.: 8826267823
Date of Submission:11-11-2011
Department of Biotechnology
School of Engineering and Technology
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Sharda University, Greater Noida
Research Article 01
SECTION A (1.5)
Title: Cardiac differentiation of embryonic stemcells by substrate immobilization of insulin-likegrowth factor binding protein 4 with elastin-likepolypeptides
Name of Authors:Ayaka Minato a, Hirohiko Ise b,*,Mitsuaki Goto c, Toshihiro Akaike b
Affiliation:
a) Graduate School of Bioscience andBiotechnology, Tokyo Institute of Technology, B-57,4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japanb )Frontier Research Center, Tokyo Institute of
Technology, 4259 Nagatsuta-cho, Midori-ku,Yokohama 226-8503, Japanc )Celagix Res Ltd., 5800-3 Nagatsuta-cho, Midori-
ku, Yokohama 226-0026, Japan
Name of Journal: BIOMATERIALS
Impact Factor: 7.882
Year in which article published: 2011
Volume: 33
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Issue: 2012
Page No.:515-523
SECTION B (1.5)
Objectives:
i.a useful strategy for cardiovascular regenerativemedicine.
ii. we report a strategy for cardiomyocytedifferentiation ofESCs using substrate immobilization of insulin-likegrowth factor binding protein 4 (IGFBP4) withelastin-like polypeptides.
SECTION C (1.5)
Statistical model used for data analysis:Cellexperiments were performed in triplicate, and theresults were expressed asmean _ standard deviation (SD). Students t testwas used to assess the statistically significant
differences in the results. A probability (p) value of
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SECTION D (1.5)
Summary: It was indicated that IGFBP4 was stablyimmobilized to polystyrene dishes by the fusion of(GVGVP)67 because the number of GVGVP repeatsneeded to be more than 67 in order to keep thehydrophobic interaction with the dishes at 37 _C.Moreover, it was suggested that (GVGVP)67-IGFBP4 that was immobilized to the dishes could
inhibit the Wnt/b-catenin signaling more stronglyand continuously than IGFBP4 as a solublefactor.These results demonstrated thatcardiomyocyte differentiation of ESCs waseffectively promoted by strong and continuousinhibition of Wnt/b-catenin signaling with(GVGVP)67-IGFBP4. Substrate immobilization ofsoluble factors with elastin-like polypeptides might
lead to the development of useful strategies forregenerative medicine of various tissues such asheart, liver, and neuron.
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