Download - Primer Blast Results

Primer-BLAST Primer-Blast resultsNCBI/ Primer-BLAST : results: Job id=JSID_01_151677_130.14.22.10_9002_primertool more...

Input PCR template Range1 - 1782Specificity of primersprimer specificity was not determined as specificity checking option was not selected.Other reportsSearch SummarySearch parameters and other detailsSearch parameter name Search parameter valueNumber of Blast hits analyzed 0Entrez queryMin total mismatches 2Min 3' end mismatches 2Defined 3' end region length 5Mismatch threshold to ignore targets 6Misprimed product size deviation 4000Max number of Blast target sequences 50000Blast E value 30000Blast word size 7Max candidate primer pairs 500Min PCR product size 70Max PCR product size 400Min Primer size 15Opt Primer size 20Max Primer size 25Min Tm 57Opt Tm 60Max Tm 63Max Tm difference 3Repeat filter AUTOLow complexity filter YesGraphical view of primer pairs

Detailed primer reports

Primer pair 1

Sequence (5'->3') Template Length Start Stop Tm GC% Self Self 3'

Primer pairs for submitted sequence

Primer-Blast results http://www.ncbi.nlm.nih.gov/tools/primer-blast/primertool.cgi

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strand complementarity complementarityForwardprimer

CGTAAGCACAACCAGGGGAT Plus 20 967 986 60.04 55.00 3.00 2.00

Reverseprimer

TAAGTGCGCCTTCGGTCTTT Minus 20 1184 1165 59.97 50.00 6.00 0.00

Internaloligo

Plus

Productlength

218

Product TmProduct Tm-min(OLIGOTm)ExonjunctionTotal intronsizeProducts on intended target

Products on allowed transcript variants

Products on potentially unintended templates

Products on target templates

Primer pair 2

Sequence (5'->3')Templatestrand

Length Start Stop Tm GC%Selfcomplementarity

Self 3'complementarity

Forwardprimer

CTGCCAACATCCTCCGACTT Plus 20 489 508 60.04 55.00 3.00 0.00

Reverseprimer

CTTCCCAACTGCCACTGCTA Minus 20 589 570 59.96 55.00 3.00 2.00

Internaloligo

Plus

Productlength

101

Product TmProduct Tm- min(OLIGOTm)ExonjunctionTotal intronsizeProducts on intended target




Primer pair 3





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Forwardprimer

TTATTGGCGGTGTGGCTCTT Plus 20 408 427 59.96 50.00 2.00 0.00

Reverseprimer

AAGTCGGAGGATGTTGGCAG Minus 20 508 489 60.04 55.00 3.00 1.00

Internaloligo

Plus

Productlength

101

Product TmProduct Tm-min(OLIGOTm)ExonjunctionTotal intronsizeProducts on intended target




Primer pair 4




Forwardprimer

GCTTGAGCGGCAATACATCG Plus 20 1131 1150 60.04 55.00 4.00 2.00

Reverseprimer

CGAATTCCCCACTGAGCCTT Minus 20 1367 1348 60.04 55.00 6.00 1.00

Internaloligo

Plus

Productlength

237





Primer pair 5




Forwardprimer

TCCATTGATGGCAGGCCTTT Plus 20 137 156 59.96 50.00 6.00 2.00


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Reverseprimer

AAGAGCCACACCGCCAATAA Minus 20 427 408 59.96 50.00 2.00 1.00

Internaloligo

Plus

Productlength

291






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