Practical Review Practice( SemI)
Advanced Placement BiologyLaboratory Review Sheet
Dissolved oxygen simulation
http://www.phschool.com/science/biology_place/labbench/lab12/varlight.htmlPlay the simulation.Relate to an aquatic ecosystemHow does dissolved oxygen relate to primary productivityHow do the following variables affect dissolved oxygen
TemperatureSalt water and fresh water?Running and still
Question and answers
If the water temperature increases, the dissolved oxygen________
DecreasesIn general running water has ________
( more or less) oxygen than still water More
Nomogram
Read the % saturation
For a temperature of 25oC, what is the % saturation ?For a temperature of 25oC, what is the dissolved oxygen?
Class Microscope. http://www.microscope-microscope.org/basic/microscope-parts.htm
Identify the followingObjective lensesCondenserMechanical stage contraolFine AdjustmentCoarse AdjustmentRotating noewpieceBe able to identify:the following
OilHighLowScanning
How to prepare a wet mount. What are these steps?
What is this cell?
Starfish egg cell
Is this cell a eukaryote or a prokaryote?
What s the difference between these two pictures of Spirogyra? How would Spirogyra be classified?
What is Volvox?
Why does this show evolution?
Spectrophotometer
http://www.umd.umich.edu/casl/natsci/slc/spec21.swf - simulation
Spectrophotometer
What is absorbance?What is percent transmittance?
What colors are associated with the wavelengths of visible light
Chromatography with a Flair
Chromatography
What is the matrix?What is the solute?What is the solvent?What is the basis of separation?What is the Rf value?
Gel electropohoresis
What is an electrophoresis chamber ?
Describe how to put the sample in the wells?
The sample is loaded at this end of the gel box
This shows the molecules moving toward the
DNA gel
What is the stain in the picture?
What is the marker used with this experiment?
DNA Electrophoresis
Which is the marker?
Micromeasurement
What is the reading on this micropipet?
Micropipet Use( review)
http://www.fhcrc.org/education/hutchlab/lessons/use.html
Review how to use the micropipet
What is an isolation streak?
What is flaming?
What is the shape of these bacteria? Which one is gram positive?
What type of bacteria are these?
What type of bacteria are these?
What type of bacteria are these?
Kirby Bauer
What is resistance , what is sensitivity?
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