Plant Tissue Plant Tissue Culturing Culturing Problem Scenario 3AProblem Scenario 3A
Problem Scenario OverviewProblem Scenario Overview
Challenged with designing a plant Challenged with designing a plant manufacturing system capable of manufacturing system capable of producing food by means of tissue producing food by means of tissue culturing.culturing.
System RequirementsSystem Requirements Limited space (approx Limited space (approx
2’x1’x1’)2’x1’x1’) Light sourceLight source Automated temp. control, Automated temp. control,
light exposure, nutrients.light exposure, nutrients. Allow for increase in plant Allow for increase in plant
sizesize Sealed sterile Sealed sterile
environment, allowing for environment, allowing for manipulation of plants and manipulation of plants and tools tools
Biological InformationBiological Information Boston fern or “Nepholepis exaltata” Boston fern or “Nepholepis exaltata”
was chosen for this problem scenario was chosen for this problem scenario because it is a variety that is easy to because it is a variety that is easy to culture, thus making it appropriate for culture, thus making it appropriate for learning tissue culturing techniques. learning tissue culturing techniques.
Organism SelectionOrganism Selection May be based on any number of criteria, depending May be based on any number of criteria, depending
on the reasons for culturing on the reasons for culturing
may include the plants ability to provide food may include the plants ability to provide food
profit from sale of the plantprofit from sale of the plant
preservation of an endangered or threatened speciespreservation of an endangered or threatened species
selection may also be based on the ability of the plant selection may also be based on the ability of the plant to be cultured, and/or the intended +method of to be cultured, and/or the intended +method of culturingculturing
Life Requirements of Life Requirements of SystemSystem
Need to address:Need to address: TempTemp Light Light Air/CO2Air/CO2 Water/Humidity Water/Humidity Nutrients/pHNutrients/pH Sterile environmentSterile environment
Technical InformationTechnical Information
This system will accommodate all the This system will accommodate all the environmental requirements of this organism environmental requirements of this organism because:because:
clean room by definition had a low level of clean room by definition had a low level of environmental pollutants, such as dust, airborne environmental pollutants, such as dust, airborne microbes, aerosol particles and chemical vapors. microbes, aerosol particles and chemical vapors.
Our system supplies nutrients, a sterile Our system supplies nutrients, a sterile environment and enough physical space to environment and enough physical space to develop.develop.
Impacts of Tissue Impacts of Tissue Culturing (pros and cons)Culturing (pros and cons)
ProsProsClean plants of infection Clean plants of infection
and quickly regenerateand quickly regenerateProduce in absence of Produce in absence of
seedsseedsConserve rare/endangered Conserve rare/endangered
speciesspeciesReproduce stress tolerant Reproduce stress tolerant
strainsstrains
ConsConsToo many of a narrow Too many of a narrow
genetic strain may limit genetic strain may limit gene pool of natural gene pool of natural populationpopulation
Threatens traditional Threatens traditional faming techniquesfaming techniques
Expensive/equipment Expensive/equipment intensiveintensive
Some varieties are difficult Some varieties are difficult to cultureto culture
Computer Control Computer Control OpportunitiesOpportunities
Some potential mechanism that could be used Some potential mechanism that could be used to monitor, run, and maintain the system would to monitor, run, and maintain the system would be:be:
*Differential pressure gage*Differential pressure gage * ThermoHygrometer (temp & humidity)* ThermoHygrometer (temp & humidity) *Air velocity monitor*Air velocity monitor *pH meter*pH meter *Light sensor*Light sensor
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