YMC GENERAL CATALOG
3Normal-Phase Columns
Types and characteristics ofnormal-phase columns
YMC-Pack SIL、SIL-06YMC-Pack CNYMC-Pack Diol-NPYMC-Pack Polyamine ⅡYMC-Pack NH2
YMC-Pack PA-GYMC-Pack PVA-SilYMC columns for SFC separation
80,81
82,8384,8586,8788,89
90909192
79
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3
Norm
al-Phase C
olumns
Types and characteristics of normal-phase columns
Product NamePore size(Å)
Particlesize
(µm)
Usable pH range Characteristics Page
YMC-Pack seriesSIL 120
3
2.0 ~ 7.5
・ Standard normal-phase column using non-polar mobile phase
・ For separation of various isomers and compounds with similar structures
82,835
10
SIL-06 60 510
CN 1205 ・ For normal-phase separation using non-polar mobile phase similar to that used with silica gel
・ Can be used in reversed-phase mode using aqueous mobile phase 84,8510
Diol-NP60
5・ For normal-phase separation using non-polar mobile phase・ Different separation characteristics from bare silica gel・ Useful for normal-phase separation (HILIC) of hydrophilic compounds
86,87120
Polyamine Ⅱ 120 5 ・ For normal-phase separation using non-polar mobile phase・ For separation of sugars and vitamins using aqueous mobile phase 88,89
NH2 120 5 ・ For normal-phase separation using non-polar mobile phase・ For separation of sugars using aqueous mobile phase 90
PA-G 120 5 4.0 ~ 7.5 ・ For separation of sugars using aqueous mobile phase 90
PVA-Sil 120 5 2.0 ~ 9.5 ・ Silica gel chemically bonded with polyvinyl alcohol・ Excellent for supercritical fluid chromatography 91
Selection of YMC-Pack CN separation modes
10 20 min
1
2
3
4
0
(S930322B)
Reversed-phase mode
Column :YMC-PackCN(5µm,120Å)250×4.6mmI.D.
Eluent :acetonitrile/water(40/60)Flowrate :0.6mL/minPressure :5.6MPaTemperature:30℃Detection :UVat254nm,0.32AUFSInjection :3µL(0.25mg/mL)
1
2
3
4
10 20 min0
(S930319D)
Normal-phase mode
Column :YMC-PackCN(5µm,120Å) 250×4.6mmI.D.
Eluent :hexane/methanol/dichloromethane(75/20/5)Flowrate :0.6mL/minPressure :2.6MPaTemperature:30℃Detection :UVat254nm,0.32AUFSInjection :3µL(0.25mg/mL)
AnexampleofanalysisusingYMC-PackCNinwhichthesamesamplesareanalyzedinbothnormal-phaseandreversed-phasemodes,isshownontheleft.YMC-PackCNcanbeusedinbothnormal-phaseandreversed-phasemodes,sinceithascyanopropylgroupsofmediumpolarity.Itcanbeusedinreversed-phasemodewithaqueousmobilephaseofhigherpolaritythanthestationaryphase.Itcanalsobeusedinnormal-phasemodewithlow-polaritymobilephasesuchashexane.Theorderofelutionwillbereverseddependingontheseparationmode.
4.
O
CH 3
O
3.
O
CH OH2
O
2.
O
CH OH
HO
2
O
1.
O
CH OH
OHHO
2
O
Progesterone
Deoxycorticosterone
Corticosterone
Hydrocortisone
High
Polarity
Low
Types and characteristics of normal-phase columnsNormal-phase chromatography is useful for the separation of positional isomers that are difficult to separate in reversed-phase mode.YMC Co., Ltd. offers normal-phase columns packed with bare silica gel or packed with silica gel modified with polar groups.
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Norm
al-Phase C
olumns
Separation characteristics of normal-phase columns
5 10 15 200 min
1
2
3
4
(A010606A)
YMC-Pack SIL(5 µm, 120 Å)
�1.�Di-n-octyl�phthalate2.�Di-n-butyl�phthalate3.�Diethyl�phthalate4.�Dimethyl�phthalate
Silica gel
Acomparisonofelutionbehaviorofvariousnormal-phasecolumnsinseparationofphthalateestersisshown.Generally,innormal-phasechromatography,thehigherthepolarityofthestationaryphasesurface,thegreatertheretentionofsolutes.Thepolarityofchemicallybondedpackingmaterialsislowerthanthatofsilicagelandthesepackingmaterialshavedifferentseparationcharacteristics.
Chemically bonded packing material
1
2 3
4
YMC-Pack Polyamine Ⅱ(5 µm, 120 Å)
5 10 150 min(A010607A)
1
2
34
YMC-Pack Diol-NP(5 µm, 120 Å)
5 100 min(A010606G)
1
2
3
4
YMC-Pack CN(5 µm, 120 Å)
5 100 min(A010606C)
Column :250×4.6mmI.D.Eluent :hexane/ethylacetate(90/10)Flowrate :1.0mL/minTemperature:30℃Detection :UVat254nm
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3
Norm
al-Phase C
olumns
Analytical columns
YMC-Pack SIL, YMC-Pack SIL-06● High quality spherical porous silica gel● Normal-phase separation due to surface silanol groups● Two different pore sizes are available● Useful for separation of compounds with similar structures
S t a n d a r d n o r m a l - p h a s e c o l u m nYMC-PackSILissuitableforseparationoffat-solublecompoundsusingnon-polarmobilephaseandseparationofpositionalisomersthataredifficulttoseparateinreversed-phasemode.SIL-06(porediameter60Å)hasgreaterabsorptionpropertiesthanSIL-12(porediameter120Å)duetoitslargerspecificsurfacearea,andis
generallyusefulforseparatingnaturalproductswithalowmolecularweight.Althoughwateroraceticacidcanbeusedasanadditivetothemobilephase,theyarelikelytobindtosilanol.Inconsiderationofseparationreproducibility,itispreferabletohaveonecolumndedicatedtothemobilephasecontainingwateroraceticacid.
■Poresize:60Å,120Å■UsablepHrange:2.0〜 7.5
1
2
3 4
10 min0
Tocopherol isomers1.�α-Tocopherol2.�β-Tocopherol3.�γ-Tocopherol4.�σ-Tocopherol
Column :YMC-PackSIL(5µm,120Å) 150×4.6mmI.D.
Eluent :hexane/THF/aceticacid(97/3/0.25)Flowrate :1.0mL/minTemperature:30℃Detection :UVat295nm
100 20
1
2
min
Hydrocortisone and prednisone
O
O
Prednisone
1.
3CH
3CH
2CH
C=O
OH
OH
O
HO
Hydrocortisone
2.
3CH
3CH
2CH
C=O
OH
OH
Column :YMC-PackSIL(5µm,120Å) 150×4.6mmI.D.
Eluent :chloroform/2-propanol/aceticacid(95/5/1)
Flowrate :1.0mL/minTemperature:30℃Detection :UVat250nm
A p p l i c a t i o n (G910523S)
A p p l i c a t i o n (K940530C)
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Norm
al-Phase C
olumns
SIL(Pore size 120 Å)
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 4.6×100 SL12S03-1046WT4.6×150 SL12S03-1546WT6.0×100 SL12S03-1006WT6.0×150 SL12S03-1506WT
S-5 120 4.6×100 SL12S05-1046WT4.6×150 SL12S05-1546WT4.6×250 SL12S05-2546WT4.6×300 SL12S05-3046WT6.0×100 SL12S05-1006WT6.0×150 SL12S05-1506WT6.0×250 SL12S05-2506WT6.0×300 SL12S05-3006WT
S-10 120 4.6×100 SL12S11-1046WT4.6×150 SL12S11-1546WT4.6×250 SL12S11-2546WT4.6×300 SL12S11-3046WT6.0×100 SL12S11-1006WT6.0×150 SL12S11-1506WT6.0×250 SL12S11-2506WT6.0×300 SL12S11-3006WT
Semi-preparative columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 10×150 SL12S05-1510WT10×250 SL12S05-2510WT10×300 SL12S05-3010WT20×100 SL12S05-1020WT20×150 SL12S05-1520WT20×250 SL12S05-2520WT
S-10 120 10×150 SL12S11-1510WT10×250 SL12S11-2510WT10×300 SL12S11-3010WT20×150 SL12S11-1520WT20×250 SL12S11-2520WT
SeeP152forpreparativecolumnsotherthanthoselistedabove.
Guard cartridge columns (3/pack)S-5 120 4.0×23 SL12S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderCartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 4.0×10 SL12S03-0104WFG5.0×10 SL12S03-0105WFG
S-5 120 4.0×10 SL12S05-0104WFG5.0×10 SL12S05-0105WFG10×30 SL12S05-0310WTG20×50 SL12S05-0520WTG
S-10 120 4.0×10 SL12S11-0104WFG5.0×10 SL12S11-0105WFG10×30 SL12S11-0310WTG20×50 SL12S11-0520WTG
SIL-06(Pore size 60 Å)
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 60 4.6×100 SL06S05-1046WT4.6×150 SL06S05-1546WT4.6×250 SL06S05-2546WT4.6×300 SL06S05-3046WT6.0×100 SL06S05-1006WT6.0×150 SL06S05-1506WT6.0×250 SL06S05-2506WT6.0×300 SL06S05-3006WT
S-10 60 4.6×100 SL06S11-1046WT4.6×150 SL06S11-1546WT4.6×250 SL06S11-2546WT4.6×300 SL06S11-3046WT6.0×100 SL06S11-1006WT6.0×150 SL06S11-1506WT6.0×250 SL06S11-2506WT6.0×300 SL06S11-3006WT
Semi-preparative columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 60 10×150 SL06S05-1510WT10×250 SL06S05-2510WT10×300 SL06S05-3010WT20×100 SL06S05-1020WT20×150 SL06S05-1520WT20×250 SL06S05-2520WT
S-10 60 10×150 SL06S11-1510WT10×250 SL06S11-2510WT10×300 SL06S11-3010WT20×150 SL06S11-1520WT20×250 SL06S11-2520WT
SeeP152forpreparativecolumnsotherthanthoselistedabove.
Guard cartridge columns (3/pack)S-5 60 4.0×23 SL06S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderCartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 60 4.0×10 SL06S05-0104WFG5.0×10 SL06S05-0105WFG10×30 SL06S05-0310WTG20×50 SL06S05-0520WTG
S-10 60 4.0×10 SL06S11-0104WFG5.0×10 SL06S11-0105WFG10×30 SL06S11-0310WTG20×50 SL06S11-0520WTG
Ordering information
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Norm
al-Phase C
olumns
Analytical columns
YMC-Pack CN● Silica gel chemically bonded with cyanopropyl groups● For both normal-phase and reversed-phase modes● Different separation characteristics from bare silica gel● Faster column equilibration than bare silica gel
Column can be used in both normal -phase and reversed-phase modesYMC-PackCNshowsretentionbehaviorofnormal-phasechromatographywhenitisusedwithlow-polaritymobilephasessuchashexane.SinceYMC-PackCNstationaryphasesurfaceislesspolarthanbaresilicagel,theretentiontimesofanalytesaregenerallyshorterthanwithbaresilicagel.YMC-PackCNisthereforeappropriateforsampleshavingtoostrongretentionwhen
analyzedusingbaresilicagel.Incontrast,YMC-PackCNshowsretentionbehaviorofreversed-phasechromatographywhenitisusedwithhighpolaritymobilephase,suchasmethanolandwater.Althoughseparationmodesareselectableaccordingtothepurposeofseparation,itispreferabletouseonecolumndedicatedforoneseparationmodeinconsiderationofthelifeofthecolumn.
■Poresize:120Å■UsablepHrange:2.0〜 7.5
1
2
3
4 5
10 200 min
Nitroaniline isomers1. 2. 3. 4. 5.
CH3 NO2 NH2NO2
NH2
NO2
NH2
NO2Toluene Nitrobenzene o-Nitroaniline m-Nitroaniline p-Nitroaniline
Column :YMC-PackCN(5µm,120Å) 250×4.6mmI.D.
Eluent :hexane/2-propanol(80/20)Flowrate :1.0mL/minTemperature:30℃Detection :UVat254nm
A p p l i c a t i o n (A010619A)
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 2.0× 35 CN12S03-H502WT2.0× 50 CN12S03-0502WT2.0× 75 CN12S03-L502WT2.0×100 CN12S03-1002WT2.0×150 CN12S03-1502WT3.0× 50 CN12S03-0503WT3.0×100 CN12S03-1003WT3.0×150 CN12S03-1503WT4.6×100 CN12S03-1046WT4.6×150 CN12S03-1546WT
S-5 120 1.0×150 CN12S05-1501WT1.0×250 CN12S05-2501WT1.5×150 CN12S05-15P5WT1.5×250 CN12S05-25P5WT2.0×150 CN12S05-1502WT2.0×250 CN12S05-2502WT4.6× 75 CN12S05-L546WT4.6×100 CN12S05-1046WT4.6×150 CN12S05-1546WT4.6×250 CN12S05-2546WT4.6×300 CN12S05-3046WT6.0×100 CN12S05-1006WT6.0×150 CN12S05-1506WT6.0×250 CN12S05-2506WT6.0×300 CN12S05-3006WT
Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 300 1.0×150 CN30S05-1501WT1.0×250 CN30S05-2501WT1.5×150 CN30S05-15P5WT1.5×250 CN30S05-25P5WT2.0×150 CN30S05-1502WT2.0×250 CN30S05-2502WT4.6× 75 CN30S05-L546WT4.6×100 CN30S05-1046WT4.6×150 CN30S05-1546WT4.6×250 CN30S05-2546WT4.6×300 CN30S05-3046WT6.0×100 CN30S05-1006WT6.0×150 CN30S05-1506WT6.0×250 CN30S05-2506WT6.0×300 CN30S05-3006WT
S-10 120 4.6×100 CN12S11-1046WT4.6×150 CN12S11-1546WT4.6×250 CN12S11-2546WT4.6×300 CN12S11-3046WT6.0×100 CN12S11-1006WT6.0×150 CN12S11-1506WT6.0×250 CN12S11-2506WT6.0×300 CN12S11-3006WT
*ShippingsolventforCNismethanol/water(60/40).Pleasetakecareofmiscibilityinnormal-phaseseparation.
Ordering information
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Norm
al-Phase C
olumns
Semi-preparative columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 10×150 CN12S05-1510WT10×250 CN12S05-2510WT10×300 CN12S05-3010WT20×100 CN12S05-1020WT20×150 CN12S05-1520WT20×250 CN12S05-2520WT
300 20×150 CN30S05-1520WT20×250 CN30S05-2520WT
S-10 120 10×150 CN12S11-1510WT10×250 CN12S11-2510WT10×300 CN12S11-3010WT20×150 CN12S11-1520WT20×250 CN12S11-2520WT
SeeP151forpreparativecolumnsotherthanthoselistedabove.
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 CN12S03-0102CC
4.0×23 CN12S03-G304CC
S-5 120 1.0×10 CN12S05-0101CC1.5×10 CN12S05-01P5CC2.0×10 CN12S05-0102CC4.0×23 CN12S05-G304CC
300 1.0×10 CN30S05-0101CC1.5×10 CN30S05-01P5CC2.0×10 CN30S05-0102CC4.0×23 CN30S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)
XPCHSMW
Cartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.0×10 CN12S05-0104WFG5.0×10 CN12S05-0105WFG10×30 CN12S05-0310WTG20×50 CN12S05-0520WTG
300 4.0×10 CN30S05-0104WFG5.0×10 CN30S05-0105WFG10×30 CN30S05-0310WTG20×50 CN30S05-0520WTG
S-10 120 4.0×10 CN12S11-0104WFG5.0×10 CN12S11-0105WFG10×30 CN12S11-0310WTG20×50 CN12S11-0520WTG
*ShippingsolventforCNismethanol/water(60/40).Pleasetakecareofmiscibilityinnormal-phaseseparation.
Ordering information
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Norm
al-Phase C
olumns
Analytical columns
YMC-Pack Diol-NP● Silica gel bonded with dihydroxypropyl group● Two pore sizes of 120 Å and 60 Å are available● Normal-phase separation using non-polar
solvents
● Useful for hydrophilic interaction chromatography (HILIC)
● Different separation characteristics from bare silica gel
D i f f e r e n t s e p a r a t i o n c h a r a c t e r i s t i c s f r o m b a r e s i l i c a g e lYMC-PackDiol-NPshowsretentionbehaviorofnormal-phasechromatographywhenitisusedwithlow-polaritymobilephases.Hydroxylgroupsonthesurfaceofthestationaryphaseactaspolargroups.YMC-PackDiol-NPisaswidely
applicabletonormal-phaseseparationassilicagel.Itisalsousefulincaseswhereseparationoptimizationisdifficulttoachieveusingbaresilicagel.Inaddition,itisavailableforHILICmodeseparationbyusingorganic/watermobilephases.
■Poresize:120Å,60Å■UsablepHrange:2.0〜 7.5
Separation characteristics of Diol-NP and SIL
5 10 15 200 minA010526A
1
2
3
4
5 6
YMC-Pack Diol-NP(5 µm, 120 Å)
5 10 150 minA010526C
1
23
4
5
6YMC-Pack SIL(5 µm, 120 Å)
Column :250×4.6mmI.D.Eluent :hexane/ethanol(80/20)Flowrate :1.0mL/minTemperature:30℃Detection :UVat254nm
DifferencesinseparationcharacteristicsofDiol-NPandSILforseparatingphenolsareshownontheleft.Diol-NPshowslongerretentionandbetterseparationthanSILinseparatingthesecompounds.
OH
OHOH
OH
HO
OH
HO
HO
OH
OH
OH
HO OH
Phenol
1.
2.
Catechol
3.
Resorcinol
4.
Hydroquinone
5.
Pyrogallol
6.
Phloroglucinol
Available as a HILIC column for separation of hydrophilic compounds
1
5
4
32
6
0 105 min15R090116P
Diol-NPisavailableforHILICmodeseparationbyusingorganic/watermobilephases.
1. Uracil 2. Uridine 3. Adenosine 4. Cytosine 5. Cytidine 6. Guanosine
NH
HN
O
O
N
HN
OH
O
OH
HOH2CO
O
N
N
N
N
OH
O
OH
HOH2C
NH2
NH
N
O
NH2
N
N
OH
O
OH
HOH2CO
NH2
HN
N
N
N
O
OH
O
OH
HOH2CH2N
Column :YMC-PackDiol-NP(5µm,120Å)150×2.0mmI.D.
Eluent :water/acetonitrile(10/90)containing10mMCH3COONH4Flowrate :0.2mL/minTemperature:30℃Detection :UVat254nm
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Norm
al-Phase C
olumns
Analytical columnsPacking material
Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
Diol-60 S-5 60 3.0×150 DN06S05-1503WT4.6×100 DN06S05-1046WT4.6×150 DN06S05-1546WT4.6×250 DN06S05-2546WT
Diol-120 S-5 120 2.0×150 DN12S05-1502WT3.0×150 DN12S05-1503WT4.6× 50 DN12S05-0546WT4.6×100 DN12S05-1046WT4.6×150 DN12S05-1546WT4.6×250 DN12S05-2546WT
Semi-preparative columnsPacking material
Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
Diol-60 S-5 60 20×150 DN06S05-1520WT20×250 DN06S05-2520WT
Diol-120 S-5 120 20×150 DN12S05-1520WT20×250 DN12S05-2520WT
*ShippingsolventforDiol-NPishexane/2-propanol(99.5/0.5).Incaseofusingeluentincludingwater,takecareofmiscibility.
N
N N
NH2
NH2
H2N
Melamine
1.Analysis of melamine in powdered cheese
(R090127P)
(R090127Q)
(R090127R)
A) Powdered cheese (blank)
C) Standard (0.5 µg/mL)
B) Melamine-spiked powdered cheese (10 mg/kg, final 0.5 µg/mL)
Powdered cheese (1 g)50% aqueous acetonitrile (5 mL)
Sonication for 30 min
Centrifugation (10,000 rpm) for 10 min
Filtration with YMC Duo-Filter (0.2 µm)
Filtration with YMC Duo-Filter (0.2 µm)
Dilution with acetonitrile or eluent
Analysis by HPLC
Extraction procedures for melamineExtraction procedures for melamine
0
60
80
40
20
mAU
min
1
1
0 4321 5
Excellent reproducibility and durability
Theabovedatashowsanalysisofmelamineinpowderedcheese,includinglot-to-lotreproducibilityandrepeatability(durability).Ingeneral,HILICcolumntendstoshowlessreproducibilityordurabilitybutDiol-NPshowsverygoodperformance.HILICcolumnlikebaresilica,zwitterionandaminotypemayadsorbioniccompounds,ontheotherhandDiol-NPhasneutralfunctionalgroupswhichreducenonspecificadsorptions.Therefore,itwouldresultinshowinggoodreproducibilityanddurability.
Lot-to-lot reproducibility
(R090625B)
(R090702A)
(R090625H)
Lot. A
Lot. C
Lot. B
Melamine
min
mAU
0
75
50
25
0 321 4
Repeatability (durability)
(R090129K)(R090129K)
(R090129G)(R090129G)
(R090129H)
(R090129I)
(R090129J)
(R090129F)(R090129F)
0 4 5321 min
1st
200th300th400th500th
100th0
100
600
500
400
300
200
mAU
Melamine
Column :YMC-PackDiol-NP(5µm,120Å)150×2.0mmI.D.
Eluent :10mMCH3COONH4/methanol(5/95)Flowrate :0.2mL/minTemperature:40℃Detection :UVat210nm
Ordering information
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Norm
al-Phase C
olumns
Analytical columns
YMC-Pack Polyamine Ⅱ● Silica gel chemically bonded with polyamine● The most suitable column for separation of sugars● Solves durability problem of conventional
silica-based amino columns
● Useful for separation of hydrophilic compounds including vitamins
● Useful for separation of fat-soluble compounds using nonaqueous mobile phase
A m i n o c o l u m n w i t h i m p r o v e d d u r a b i l i t yYMC-PackPolyamineIIisasilica-basedpackingbondedwithpolyamine.Itisparticularlyusefulforseparationofsugars.ThecolumnlifetimeofYMC-PackPolyamineⅡinaqueousmobilephaseislongerthanconventionalsilica-basedaminocolumns,andthusis
applicabletoseparationofoligosaccharidesusingmobilephasewithrelativelyhigherwatercontent.Inaddition,YMC-PackPolyamineⅡcanbeusedtoseparateioniccompoundswithacombinationofnormal-phasemodeandweakanionexchangemode.
■Poresize:120Å■UsablepHrange:2.0〜 7.5
Excellent durability
0 50 100
10
20
30
Fructose
Glucose
Sucrose
Maltose
Lactose
Continuous flow of water (time, hr)
Retention time (min) 〈Flowconditions〉
Column :YMC-PackPolyamineⅡ 250×4.6mmI.D.
Eluent :waterFlowrate :1.0mL/minTemperature:ambientTime :100hours
〈Analyticalconditions〉Eluent :acetonitrile/water(75/25)Flowrate :1.0mL/minTemperature:26℃Detection :RI,32×10-6RIU/FS
Eveninahardflowdurabilitytestusingamobilephasethatconsistsonlyofwater,theretentiontimeofsugarschangeslittle.
The most suitable column for separation of sugars, including oligosaccharides
10 20 30 40 min0
1 2
3
45
1. Maltoundecaose(G11)2. Maltododecaose(G12)3. Maltotridecaose(G13)4. Maltotetradecaose(G14)5. Maltopentadecaose(G15)
(T910718A)
Column :YMC-PackPolyamineⅡ 250×4.6mmI.D.Eluent :acetonitrile/water(55/45)Flowrate :1.0mL/minTemperature:26℃Detection :RI,32×10-6RIU/FS
1
23
45
1. Fructose2. Glucose3. Sucrose4. Maltose5. Lactose
(F030618H)10 20 30 min0
Column :YMC-PackPolyamineⅡ 250×4.6mmI.D.Eluent :acetonitrile/water(75/25)Flowrate :1.0mL/minTemperature:25℃Detection :RI,32×10-6RIU/FS
第03章.indd 88 11.1.28 10:49:28 AM
89
3
Norm
al-Phase C
olumns
Elution volume of sugars and sugar alcohols
0
5
10
15
20
25
30
35
40
45
50
55
60
65
Glycerol
ErythritolFucose
Xylose
Xylitol
Rhamnose
Arabitol
Frucutose
Mannose(※)
Sorbitol
Mannitol
Glucose
Galactose(※)
Sucrose
Inositol
Maltitol
Maltose
Lactose
Raffinose
Palatinose
Sucralose
Ribitol
75% 80% 85%
Lactitol
Palatinit
Acetonitrile concentration (%)※Not detected with 85% acetonitrile
Elution volume (mL)
YMC-PackPolyamineⅡisapplicableforseparationoffat-solublevitaminsandwater-solublevitaminsasanormal-phasecolumnthatcanbeusedwithwaterorbufferandvariousorganicsolvents.
For normal-phase separation
10
1
2
3
4
0 20 30 min (T920728E)
O
CH
HO
H C
3
CH 3
3
α-Tocopherol
1.
O
HO
H C
CH 3
3
γ-Tocopherol
3.
O
CH
HO3
CH 3
β-Tocopherol
2.
O
HO
CH 3
σ-Tocopherol
4.
Column :
Eluent :
Flow rate :Temperature :Detection :
YMC-Pack Polyamine Ⅱ250×4.6 mmI.D.hexane/ethly acetate(70/30)1.0 mL/min30 ℃UV at 295 nm
Tocopherol isomers
(A970603A)min
1
2 3
0 10 20
1. Nicotinic acid2. Erythorbic acid3. L-Ascorbic acid
Column :
Eluent :Flow rate :Temperature :Detection :
YMC-Pack Polyamine Ⅱ250×4.6 mmI.D.acetonitrile/50 mM NH4H2PO4 (70/30)1.0 mL/min30 ℃UV at 250 nm
Water-soluble vitamin
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.6×150 PB12S05-1546WT4.6×250 PB12S05-2546WT6.0×150 PB12S05-1506WT6.0×250 PB12S05-2506WT
Semi-preparative columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 10×250 PB12S05-2510WT20×250 PB12S05-2520WT
Guard cartridge columns (three-pack)S-5 120 4.0×23 PB12S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderCartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsS-5 120 4.0×10 PB12S05-0104WFG
5.0×10 PB12S05-0105WFG10×30 PB12S05-0310WTG20×50 PB12S05-0520WTG
Ordering information
第03章.indd 89 11.1.28 10:49:31 AM
90
3
Norm
al-Phase C
olumns
Analytical columns
YMC-Pack NH2
● Silica gel chemically bonded with aminopropyl groups● Useful for separation of sugars● Enables normal-phase mode separation using aqueous or nonaqueous mobile phase
N o r m a l - p h a s e s e p a r a t i o n c o l u m n u t i l i z i n g a m i n o g r o u p sYMC-PackNH2isanormal-phaseseparationcolumnutilizingthepolarityofprimaryaminogroups.Itisalso
applicabletoseparationsutilizingweakanionexchange.YMC-PackNH2isoftenusedforseparationofsugars.
■Poresize:120Å■UsablepHrange:2.0〜 7.5
YMC-Pack PA-G● Silica gel chemically bonded with polyamine● Useful for separation of acidic oligosaccharides
N o r m a l - p h a s e s e p a r a t i o n c o l u m n u t i l i z i n g a m i n o g r o u p sYMC-PackPA-Gisusefulforseparationofacidicoligosaccharides.YMC-PackPA-GissimilarselectivityasYMC-PackPA.
■Poresize:120Å■UsablepHrange:4.0〜 7.5
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.6×100 NH12S05-1046WT4.6×150 NH12S05-1546WT4.6×250 NH12S05-2546WT4.6×300 NH12S05-3046WT6.0×100 NH12S05-1006WT6.0×150 NH12S05-1506WT6.0×250 NH12S05-2506WT6.0×300 NH12S05-3006WT
Semi-preparative columnsS-5 120 10×150 NH12S05-1510WT
10×250 NH12S05-2510WT10×300 NH12S05-3010WT20×150 NH12S05-1520WT20×250 NH12S05-2520WT
SeeP153forpreparativecolumnsotherthanthoselistedabove.
Guard cartridge columns (three-pack)Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.0×23 NH12S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderCartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.0×10 NH12S05-0104WFG5.0×10 NH12S05-0105WFG10×30 NH12S05-0310WTG20×50 NH12S05-0520WTG
Ordering information
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.6×150 PG12S05-1546WT4.6×250 PG12S05-2546WT
Ordering information
Guard columnsS-5 120 4.0× 10 PG12S05-0104WFG
第03章.indd 90 11.1.28 10:49:32 AM
3
Norm
al-Phase C
olumns
91
Analytical columns
YMC-Pack PVA-Sil● Vinyl alcohol polymerised silica● High stability and reproducibility● Excellent choice for SFC (Supercritical Fluid Chromatography)
P o l y v i n y l a l c o h o l f u n c t i o n a l i z e d s i l i c aYMC-PackPVA-Sil,bondedwithamonomolecularpolymercoatingofvinylalcohol(PVA),completelycoversbothexternalandinternalsurfacesofthesilicasupport,protectingitagainstaggressive,highpHbuffersand
solvents.ThePVApolymershellonPVA-Sildeactivatesthesilicasupportwhileprovidingahydrophilicsurface.PVA-SilcolumnarealsowellsuitedforSFCseparations.(seepage92forSFC.)
■Poresize:120Å■UsablepHrange:2.0〜 9.5■RecommendedpHrange:2.0〜 7.5
0
Time (min)
SE
S ASG
DG MGDGDGDG
PE
PG PI
PC
SGCER
? ?
8 16 24 32 40
Potato Lipids
Column :YMC-PackPVA-Sil(5µm,120Å),250×4.6mmI.D.Flowrate :1to2mL/minMobilePhase:A)2-methylpentane/methyltert-butylether(98/2)
B)2-propanol/acetonitrile/CHCI3/CH3COOH(84/8/8/0.025)C)2-propanol/water/triethylamine(50/50/0.2)
Gradient Tmin:0 5 15 40 40.1 45 50 B%:0 20 52 52 70 0 0 C%:0 0 4 14 0 0 0 Flow(mL/min):1 1 1 1.4 1.4 2 2
Nebulisertemperature:25℃,Evaporationtemperature:35℃Detector:ELSDSampleS:Sterols CER:CerebrosidesSE:SterolEsters SG:SterylglycosidesMGDG:Monogalactosyldiacylglycerols DGDG:DigalactosyldiacylglycerolsPE:Phosphatidylethanolamine PG:PhosphatidylglycerolsPC:Phosphatidylcholine ASG:AcylsterylglycosidesPI:Phosphatidylinositol DG:Diacylglycerol
A p p l i c a t i o n Literature:W.W.Christie;R.A.Urwin,J.HighResol.Chromatogr.,Vol.18(1995)P97-100
0 5 10 15 20 min
0
5
10
15
20
25mAU
1
23
4
5
6
Nucleotides (in HILIC mode)1.�5'-TMP2.�5'-UMP3.�5'-IMP4.�5'-AMP5.�5'-GMP6.�5'-CMP
Column :YMC-PackPVA-Sil(5µm,120Å)150×2.0mmI.D.
Eluent :acetonitrile/100mMCH3COONH4(75/25)Flowrate :0.2mL/minTemperature:40℃Detection :UVat254nm
A p p l i c a t i o n (R100208K)
Ordering information
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.6× 50 PV12S05-0546WT4.6×100 PV12S05-1046WT4.6×150 PV12S05-1546WT4.6×250 PV12S05-2546WT
Guard cartridge columns (three-pack) S-5 120 4.0×23 PV12S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderCartridge holder(inner diameter:4.0 mm)
XPCHW
第03章.indd 91 11.1.28 10:49:36 AM
92
3
Norm
al-Phase C
olumns
Analytical columns
YMC columns for SFC separation● 11 Selectivity Options● Quality Control via SFC evaluation● Below columns stocked for immediate
delivery
● 3 µm also available● Please inquire for preparative
versions and additional analytical configurations
Efficient separation of achiral APIs (Active Pharmaceutical Ingredients) in SFC modeInrecentyears,SFChasgainedenormouslyinimportanceandacceptanceinthepharmaceuticalindustry.Nowadays,itisroutinelyusedforthepurificationofenantiomers.Inaddition,SFChasassumedarelevantrolebothinanalyticalseparationsandlargerscalepurificationofachiralcompounds
asitisseenasaneconomicand"green"alternativetoclassicalHPLC.WehaveabundantpackingmaterialsforachiralSFCseparations.InabroadrangeofdifferentSFCselectivitythatcansolvethemostdiverseseparationproblems.
Packing material
Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
2-ethyl pyridine S-5 60 4.6×250 EP06S05-2546PS
Diol S-5 60 4.6×250 DP06S05-2546PS
120 4.6×250 DL12S05-2546PS
diethylaminopropyl S-5 60 4.6×250 DE06S05-2546PS
pyridine amide S-5 60 4.6×250 PY06S05-2546PS
propyl acetamide S-5 60 4.6×250 PP06S05-2546PS
Packing material
Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
silica S-5 120 4.6×250 SL12S05-2546PS
Polyamine II S-5 120 4.6×250 PB12S05-2546PS
amino S-5 120 4.6×250 NH12S05-2546PS
cyano S-5 120 4.6×250 CN12S05-2546PS
PVA-Sil S-5 120 4.6×250 PV12S05-2546PS
1
2
345
6
7
8
9
10
PDA-230 nmMIX500
450400350300250200150100500
0 1 2 3 4 5 6 7Minutes
8 9 10 11 12 13 14
mV
1. Citral2. Lidocaine3. Ibuprofen4. Caffeine5. Amitriptyline6. Propranolol7. Sulfinpyrazone8. Procainamide9. Cytosine
10. Sulpiride
A p p l i c a t i o n
Column :YMC-PackPVA-Sil(5µm,120Å)250×4.6mmI.D.Flowrate :3.5mL/minOutletpressure:15MPaColumntemp.:40℃Detection :230nmInjectionvolume:5µLMobilephase:CO2withmodifierModifier :Methanolw/0.1%DiethylamineGradientelution:linearchangefrominitial5%modifier-65%
modifierover12.5minutes
1
2
3
0
200
400
600
800
0 1 2 3Minutes
4 5 6 7
mA
U
Q u a l i t y c o n t r o l c h e c k b y S F C
Column :YMC-PackDiol(5µm,120Å)250×4.6mmI.D.Mobilephase:80/20CO2/MethanolFlowrate :2.0mL/minOutletpressure:10MPaColumntemp.:40℃Detection :230nmInjectionvolume:5µL
QCmixcomponents1.Acetophenone2.Caffeine3.Thymine
Ordering information
第03章.indd 92 11.1.28 10:49:37 AM
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