MSU Amy Iezzoni (PD) Jim Hancock Dechun Wang Cholani Weebadde
WSU Cameron Peace Dorrie Main Kate Evans Karina Gallardo Vicki
McCracken Nnadozie Oraguzie Former WSU Raymond Jussaume Mykel
Taylor Cornell Susan Brown Kenong Xu Clemson Ksenija Gasic Gregory
Reighard Texas A&M Dave Byrne Univ. of CA-Davis Tom Gradziel
Carlos Crisosto Univ. of New Hamp. Tom Davis Univ. of Minnesota Jim
Luby Chengyan Yue Oregon State Univ. Alexandra Stone Plant Research
Intl, Netherlands Eric van de Weg Marco Bink USDA-ARS Nahla Bassil
Gennaro Fazio Chad Finn Univ. of Arkansas John Clark
Slide 2
Acknowledgements This project is supported by the Specialty
Crop Research Initiative of USDAs National Institute of Food and
Agriculture
Slide 3
Plant Research International, Netherlands East Malling
Research, UK CRA-FRU, Rome, Italy IASMA, Trento, Italy INRA (
Bordeaux, Avignon, Angers ), France Andres Bello University, Chile
Plant & Food Research, New Zealand ARC, South Africa RosBREED
participant location International Collaborators + 2 Scientific
Advisory Panel members (Spain, New Zealand)
Slide 4
Discovering a QTL A major milestone or a stepping-stone into
practical marker-assisted breeding? Cholani Weebadde 25 July 2013
ASHS, Palm Desert, CA
Slide 5
Outline of Presentation How does QTL discovery inform MAB? The
RosBREED MAB pipeline - a strategy to put DNA knowledge into
practical application Workshop presentations
Slide 6
How does QTL discovery inform Marker-Assisted Breeding
(MAB)?
Slide 7
Which wild germplasm to use? Which traits to target? Which
parents & combinations to create? Which seedlings to progress?
Which selections to trial? Which advanced selections to
commercialize? What is Marker-Assisted Breeding? Use of DNA
information to support breeding decisions
Slide 8
What published QTLs look like A bump on the chromosome What
does it tell the breeder??? Nothing much really! Just that there
may be a gene in the bump region of the chromosome that is
associated with the trait
Slide 9
Known QTLs and their application Hundreds of published QTLs are
currently available for Rosaceae crops Of these, only a few were
being used for MAB
Slide 10
RosBREED has bridged this chasm Genomics Resources Marker-
assisted breeding 100s of QTLs published More efficient development
of new cultivars RosBREED is dedicated to putting DNA knowledge
into practical application to increase the efficiency of rosaceous
crop breeding
Slide 11
RosBREED Mission Statement We will develop and apply
marker-assisted breeding, based on improved knowledge of industry
value and consumer preferences, to accelerate and increase the
efficiency of rosaceous cultivar release and successful cultivar
adoption 4 yrs $7.2M federal $7.2M matching Amy Iezzoni, Michigan
State Univ. Cameron Peace, Wash. State Univ. This project is
supported by the Specialty Crop Research Initiative of USDAs
National Institute of Food and Agriculture
Slide 12
Demonstration Breeders & Breeding Trainees
Slide 13
The RosBREED MAB Pipeline: a strategy to put DNA knowledge into
practical application. How we bridged the Chasm.
Slide 14
Find An individuals genome is the full complement of genetic
information that it inherited from its parents. Within this vast
repertoire of genetic information, individuals genes are being
discovered that control critical production and fruit quality
traits. Put into application in breeding Jewels = valuable genetic
discoveries ready for breeding application = marker-locus-trait
associations validated in a breeding program Polish Jewels in the
genome analogy
Slide 15
Lots of Shiny Rocks! So What?!! Each breeder needs to appraise
for themselves all these gemstones to decide which ones are worth
polishing www.rocktumbler.com to find the jewels!
Slide 16
Socio-Economics Surveys (example for apple) Breeders Market
Intermediaries Michigan Producers Washington Producers % of
respondents MOST important traits LEAST important traits So that
resources for development of genetic tests (polishing the shiny
rocks) can be directed to traits with the highest importance to
stakeholders.
Slide 17
Integrate DNA information with conventional breeding,
systematically (8-stage MAB Pipeline) RosBREED MAB Pipeline
Slide 18
Jewels in the Genome Available Now!
Slide 19
Information on the jewels on eXtension RosBREED Jewels are now
featured in eXtension
Slide 20
Workshop presentations
Slide 21
Breeding markers DNA testing services QTL priorities Crossing
schemes Trial MASS Simple validation Allele effects &
distributions Seedling selection schemes RosBREED success stories:
QTL and QTL allele validation in Cherry, Apple and Peach 1.Amy
Iezzoni Cherry 2.Sujeet Verma Apple 3.Ksenija Gasic - Peach
Slide 22
Breeding markers DNA testing services QTL priorities Crossing
schemes Trial MASS Simple validation Allele effects &
distributions Seedling selection schemes Marker-Assisted Breeding
for Apple: Experiences from University of Minnesota and Washington
State University Jim Luby RosBREED Breeding Team Lead