Modeling of phyB-dimers(indicates that PfrPfr homodimers are the dominant signalling components)
Filippo Venezia
Diplom biologist (t.o.)
ZBSA
Superviser: Dr. Fleck
Prof. Schaefer
Dimer model
k2 =
€
σ fr N
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σ rk1 = N Photo-conversion rate
ks = synthesis
kdr,fdr = degradation kr = dark
reversion
Pfr-Pfr - D2
Pfr-Pr - D1
Pr-Pr - D0
Simplified phyB pool dimer model
New unknown parameters: k3*, k4
*, kr*
Which are the physiological
functional pool?
Which effects have the new unknown parameters on the
system?
Model selection
Poolkombination:
RSS X2 AIC BIC F-test
D2ns + D2n 0.0613 0.09 -57.26 -59.67 1.083D1ns + D1n 0.972 3.563 -27.25 -29.66 1.81D1+ D2 0.0895 0.128 -53.15 -55.56
1.118 (Pfr-Pool)
f0.9=1.69
Pfr totalPfr-Pfr
n=20
Red = 699nmIndigo = 692nmBlack = 687nmBlue = 681nmGreen = 672nm
Approximation to dark reversion experimental data
Identificable Parameter
kr = 1.5333
krr = 0.0989
X2 = 0.0158
kr/krr = 15.5
n=10
Indigo = OhrGFP/A-B-Blue = ABO/A- on iceRed = calc. Without dark reversionBlack = fitted with dark reversionGreen = ABO/A-
Approximation without speckles
Model without speckles cannot fit the data
Red = 699nmIndigo = 692nmBlack = 687nmBlue = 681nmGreen = 672nm
Summary Dimer model can describe photon-flux response curve sufficiently
The optimal Model explaining Pfr-Pfr homodimer data is obtained through the use of selection methods.
Speckles are essential for stabilizing dark reversion
phyB dimers shows individual dark reversion kinetics.
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