Microbiology Practical I
The following slides were contributed by your fellow BIO 251 students…
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Lab 1: Eukaryotes and Microscopy
Know the parts of the microscope…
Lab 1: Eukaryotes and Microscopy
Paramecium caudatum
Lab 1: Eukaryotes and Microscopy
Euglena gracilis
Lab 1: Eukaryotes and Microscopy
Amoeba proteus
Lab 1: Eukaryotes and Microscopy
Fungus – Saccharomyces cerevisiae
Lab 1: Eukaryotes and Microscopy
Cyanobacteria ~ Nostoc
~Bacteria slides~
Micrococcus luteus
~Bacteria Slides~
Bacillus subtilis
~Bacteria Slides~
Bacillus anthracis
~Bacteria Slides~
Rhodospirillum rubrumSorry not in focus…
~Bacteria Slides~
See other file for Gram Stain, Endospore, Acidfast, and Capsule Staining…
[ Adobe Acrobat of Gram and Endospore ]
Some Endospore Staining ~
Streak Plate:
Know colony characteristics (color, elevation, margin, etc.)
Spread Plate vs. Streak Plate:
Streak Plate:
Spread Plate:Mixed – Bacillus subtilis & Serratia marcescens
Practical I Unknown Tests:
~ Simple Stain~ Gram Stain~ Endospore Stain~ Acid-Fast Stain (Demo)~ Capsule Stain (Demo)~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep)
~ Obligate Aerobe, Facultative Anaerobe, Aerotolerant Anaerobes, Microaerophiles (Gas Pak, Eugon Agar, Thioglycollate Broth)
Gas Pak Anaerobic vs. Aerobic:
Dilution Problems:
Dilution Problems:
Bacterial Growth Curve:Make sure you know how to:
Use a spectrophotometer to measure absorbance.
Produce a log graph of the calculations.
Calculate generation time from a growth curve.
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