USA – Biotech USA – [email protected] – SWEDEN – Biotech AB – [email protected] – INDIA – Biotech Pvt Ltd – [email protected]
KNIttED opEN tUbUlAr rEActIoN DElAy AND mIxINg coIlSKOT – Tubular COils
the Kot reactor is the most optimal DelayElement for Use in Analytical Flow Systems
KOT – Tubular COils
KNIttED opEN tUbUlAr rEActIoN DElAy AND mIxINg coIlS• Tortousflowpathofoptimalflowdynamicdesignmadefrom
totallyinertPTFE
• Minimisedaxialdispersioncombinedwithefficientradialmixing
• Availableindividuallyindifferentlengthsandinnerdiameters
preserved chromatographic peak ShapeTheBiotechknittedopentubular(KOT)reactorsaremadefromtubingwhichhasbeenknittedintoatortu-ouspathtoforcethefastmovingcentreoftheliquidstreamtomixradiallywiththeslowermovingbound-aryliquidlayer,therebyminimizingtheaxialdisper-sion.Theshapeofachromatographicpeakenteringthereactoristhuspreservedveryefficiently.ToensureastableradialmixingwithintheKOT,alinearflowrateof10cm/sormoreisrecommended.TheKOTreactorisusuallythemostoptimaldelayelementforuseinanalyticalflowsystems,andistypicallyinsertedintheflowpathtocreateadelayline,sothatareactionor
othereventthatrequiresacertaintimecantakeplace.DifferentdelayorreactiontimesareaccomplishedbychangingtheinnerdiameterandlengthoftheKOT,takingtheflowratethroughtheKOTintoconsider-ation.
Knitted Open Tubular Reactors of PTFE
Length ID 0.25 mm ID 0.50 mm ID 0.75 mm m
1 3000-123 3000-125 3000-1282 3000-223 3000-225 3000-2284 3000-423 3000-425 3000-428
10 3000-133 3000-135 3000-13820 3000-233 3000-235 3000-238
KOT - Tubular CoilsKNITTED OPEN TUBULAR REACTION DELAY AND MIXING COILS
Tortous flow path of optimal flow dynamic design made from totally inert PTFEMinimised axial dispersion combined with efficient radial mixingAvailable individually in different lengths and inner diameters
Preserved Chromatographic Peak ShapeThe SeQuant knitted open tubular (KOT) reactors are made from tubing which has been knittedinto a tortuous path to force the fast moving centre of the liquid stream to mix radially with theslower moving boundary liquid layer, thereby minimizing the axial dispersion. The shape of achromatographic peak entering the reactor is thus preserved very efficiently. To ensure a stableradial mixing within the KOT, a linear flow rate of 10 cm/s or more is recommended. The KOTreactor is usually the most optimal delay element for use in analytical flow systems, and is
Example Application AreasSeQuant KOT reactors can be applied as a delay line in chromatographic separations. Oneexample being the parallel coupling of a MS detector and a fraction collector in preparativeseparations (see left figure above). The delay time induced by the KOT allows the detector toanalyse the eluate and make intelligent decisions of when a new fraction is going to be initiated,without loss of chromatographic efficiency.The SeQuant KOT reactors can also be used in post-column reaction detection in HPLC (seeright figure above), using both room temperature and heated chemistries. In this set-up theKOT also ensure thorough mixing between the column effluent and the added reagents. Typicalreactions are the production of fluorescent products from reactions between eluted compoundsand reagents without native fluorescence.
A KOT applied as a delay coil allowing the result fromthe MS to control the fraction collector
Typical use of a KOT as a mixer and reactor in HPLCpost-column reaction detection
Ordering InformationThe SeQuant KOT reactors are made from translucentpoly(tetrafluoroethene) [PTFE], which is the most inert andresistant polymer available. The SeQuant KOT reactors arealso available in black PTFE and fluorinated ethylene propylene[FEP]. Other options are available upon request.
Recommended Minimum Flow Rate
ID Linear Flow Volumetric Flowmm cm/s mL/min
0.25 10 0.290.50 10 1.20.75 10 2.7
typically inserted in the flow path to create adelay line, so that a reaction or other event thatrequires a certain time can take place. Differentdelay or reaction times are accomplished bychanging the inner diameter and length of theKOT, taking the flow rate through the KOT intoconsideration.
The KOT Reactor is the Most Optimal DelayElement for Use in Analytical Flow Systems
SeQuantInnovators in Chemical Analysis™
Product Sheet 3000-103-040908-TJN
MS
FractionCollector
Separation Column KOTSplit
SeQuant AB · Box 7956 · S-907 19 Umeå · SwedenTel +46-90-154880 · Fax [email protected] · www.sequant.com
Detector
Reagent Supply
Separation Column KOT
Tee
AKOTappliedasadelaycoilallowingtheresultfromtheMStocontrolthefractioncollector
TypicaluseofaKOTasamixerandreactorinHPLCpost-columnreaktionreactiondetection
Tee
SeparationColumn
ReagentSupply
DetectorKOTSeparationColumn Split KOT
Fraction Collector
MS
Example Application AreasBiotechKOTreactorscanbeappliedasadelaylineinchromatographicseparations.OneexamplebeingtheparallelcouplingofaMSdetectorandafractioncollec-torinpreparativeseparations(seeleftfigureabove).ThedelaytimeinducedbytheKOTallowsthedetectortoanalysetheeluateandmakeintelligentdecisionsofwhenanewfractionisgoingtobeinitiated,withoutlossofchromatographicefficiency.
TheBiotechKOTreactorscanalsobeusedinpost-columnreactiondetectioninHPLC(seerightfigureabove),usingbothroomtemperatureandheatedchemistries.Inthisset-uptheKOTalsoensurethor-oughmixingbetweenthecolumneffluentandtheaddedreagents.Typicalreactionsaretheproductionoffluorescentproductsfromreactionsbetweenelutedcompoundsandreagentswithoutnativefluorescence.
NormAl INStrUmENtAtIoN oF A UV photorEActor IN AN lc INStrUmENt.
photooxIDAtIoN oF phENylAlANINEtypIcAl pc-Ic chromAtogrAm
Peak
Hei
gh
t(m
V)
MC
AA
(14
pp
b)
MB
AA
(14
pp
b)
DC
AA
(18
pp
b)
DB
AA
(15
pp
b)
TCA
A(
16p
pb
)
Time(min)
ww
w.s
om
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.se
USA – Biotech USA – [email protected] – SWEDEN – Biotech AB – [email protected] – INDIA – Biotech Pvt Ltd – [email protected]
BiotechAB.P.O.Box133,SE-43923Onsala,Sweden.
Telephone+46(0)300-569180.Fax+46(0)300-569181
[email protected]–www.biotech-sweden.com
P/N KOT - Tubular coils3000-123 KnittedOpenTubular(KOT)Reactor,PTFE,1m,0.25mmID
3000-125 KnittedOpenTubular(KOT)Reactor,PTFE,1m,0.5mmID
3000-128 KnittedOpenTubular(KOT)Reactor,PTFE,1m,0.75mmID
3000-223 KnittedOpenTubular(KOT)Reactor,PTFE,2m,0.25mmID
3000-225 KnittedOpenTubular(KOT)Reactor,PTFE,2m,0.5mmID
3000-228 KnittedOpenTubular(KOT)Reactor,PTFE,2m,0.75mmID
3000-423 KnittedOpenTubular(KOT)Reactor,PTFE,4m,0.25mmID
3000-425 KnittedOpenTubular(KOT)Reactor,PTFE,4m,0.5mmID
3000-428 KnittedOpenTubular(KOT)Reactor,PTFE,4m,0.75mmID
3000-133 KnittedOpenTubular(KOT)Reactor,PTFE,10m,0.25mmID
3000-135 KnittedOpenTubular(KOT)Reactor,PTFE,10m,0.5mmID
3000-138 KnittedOpenTubular(KOT)Reactor,PTFE,10m,0.75mmID
3000-525 KnittedOpenTubular(KOT)Reactor,PTFE,5m,0.5mmID
MostdimensionsalsoavailableinblackPTFE-intendedforlightsenstivecompoundsOtherKOTsmightbecustommanufactured,pleasecontactBiotechABforfurtherdetails
plEASE INForm mE AboUt prIcES AND DElIVEry tErmS
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