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HONEYBEEINSEMINATIONSERVICE

SCHLEYINSTRUMENTShoneybeeinsemination.com

Contact:SusanCobeyscobey@mac.com

STANDARDMODELI02 ECONOMICALMODELI01

TheSchleyInseminationInstrumentsareprecisionmachined,highlyadjustableandprovideprecisioninfineandaccuratemovementswithhighrepeatabilityandlowmaintenance.Theinstrumentsconsistofasturdybasesupporting;ball&socketassembliestoprovidesmoothmovementofhooks–aventralhookandpressuregripsforcepsorperforatedstinghook;asyringemanipulatorandsyringe;andaqueenholderassembly-includingthesetofqueen&backuptubes.Partsaremadeofstainlesssteelmaterialandthebaseisbrasswithaplatedfinish.Thechoiceofvarioustypesofsyringescanbeusedwithbothmodels:thestandardSchleysyringe,TheHarbolargecapacitysyringeandtheSchleymodifiedHarbo-typeofsyringe.STANDARDMODELI02ThestandardModel102instrumenthasaheavy,sturdybaseforstability.Theball&sockethookholderassembliesareheavydutywithmoveablecolumnsforeasyadjustments.Thequeenbeeholderassemblyisalsomoveableforeasyadjustments.TheinstrumentcomeswiththeforcepspressuregriphookandthestandardSchleysyringe,unlessotherwisespecified.TheperforatedstinghookandthestandardHarbolargecapacitysyringearecompatibleandavailable.ECONOMICALMODELI01TheModel101instrumentislighterinweight,hasaflatbasethatcanbesecurelyscrewedinplaceforstability.Theball&sockethookholderassembliesaresmallerwithfixedcolumnsinperfectalignment.Thequeenbeeholderassemblyissimplified,althoughadjustable.Choiceofstinghooks-theforcepspressuregriphookorperforatedstinghookareavailable.Choiceofthesyringetypeisalsoavailable-thestandardSchleysyringeortheSchleymodifiedHarbo-typeofsyringe.ThestandardHarbolargecapacitysyringeisalsocompatibleandavailablewiththis.

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STANDARDMODELI02BasicInstrumentsPartsBothInstrumentmodelshavesimilarfunctionalparts.1–Standbase2-Leftsupportpostwithhookholder,ballbearingblock3-Rightsupportpostwithhookholder,ballbearingblockandsyringemanipulator.4–QueenholderassemblywithCO2connection5–SchleyInseminationsyringewithglasstip6-Micromanipulatordriveforthesyringe7–Ventralhook8–PressuregripforcepshookwithpressbuttoncontrolTocleantheinstrumentsuseadampclothwithalcohol.

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TheSchleylargecapacitysyringe(modeledaftertheHarbosyringe)

Tomeasurethesemendosageinthe1cmglasstube,asingleorpartialrotationequals:

½=6µl3/4=8-9µl1 =12µl

TheHarboLargeCapacitySyringe(notpictured)hasamicrometertoaccuratelymeasurethesemendosage.Thetipconnectionprovidesflexibilityofthetip.Thissyringeiscompatiblewithbothinstruments.Pleaseseetheinstructionmanualfordetails.

TensionAdjustmentoftheHooks,Model1022a:Screwtoadjusttensionontheball.2b:TwoORingssecureeachhook.Greasethesewithsilicone.

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TensionAdjustmentoftheHooks,Model101Toadjusttheballtensionforthehookholderblock-Loosenthescrew,pressdownontheblocksupportandtightenatthedesiredtension.

HeightAdjustmentofQueenHolderTube,Model1024a:TheCO2deliverytubeisadjustableinheight.4b:Thequeenholdingtubesaremadeofacrylic.Cleanqueentubeswithsoapandwater.Donotoverheatorsoakinalcohol.

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4c:TheheightofCO2deliverytubeisadjustableinheight,securedbythesetscrewintheModel102instrument.

SyringeDriveTensionAdjustment6a:Toadjustthesyringedrivetension-Turnthisnutcounterclockwiseto

tightenandclockwisetoloosen.6c:Blocksupportring.Donotadjusttootightly.

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6b:Thelockwasherunderthescrewreducestherotarymotion.Thesidescrewservesthissamefunction.

Ventralhook.Cleanwithalcohol

DorsalPerforatedHook.Cleanwithalcohol

DorsalPressureGripForcepsCleanwithalcoholanddry.Forstorage,coatwithsiliconegrease.

ToadjusttheMicromanipulatorsyringedrivetensioninthestandardoldermodelinstrument(whichlacksthe6aadjustmentscrew):Removethedrivescrewandplacepressureonthespringdiskwhiletighteningthesetscrew.