Laboratory Conference
Group II
Atienza, Abegail
Comia, Ralph Joseph
Castillon, Kristine
Expt. C : HMP on SMA
• Herbal Plant: Makahiya 30%
• Preparation: Decoction
• Procedure: SD 30% = 30gm/100ml = 300mg/1ml 30 grams of Makahiya 100 ml of water Boil up to 100C RD= 5mg/gm
• Animal: 5 albino mice Weight: 1-5 mice: 20 grams
20 gm 100mg x 1mL = 0.33mL
X 5mg/gm 300mg
100 mg
• Observation/ Parameters
• (F) FREQUENCY No. of movements/ minute Actual count/ min (ave.)
(A) AMPLITUDE Force of movement/ minute (+) slow (++) moderate (+++) strong
Procedure
• Place the 5 albino mice on the pool• Observe and acclimatize for 30 minutes before
the administration of drugs (Frequency and Amplitude)
• Administer the drug intraperitoneally• Observe every 15 minutes thereafter.
Mice Baseline 15 mins after 30 mins. After 45 mins. After 60 mins. After
Frequency
Amplitude
Frequency
Amplitude
Frequency
Amplitude
Frequency
Amplitude
Frequency
Amplitude
Ave 35 +++ 30 ++ 28 ++ 25 + 15 +
Result
Discussion
• Makahiya(scientific name: Mimosa pudica)
• creeping annual or perennial herb
• the compound leaves fold inward and droop when touched, re-opening within minutes.
• Common weed widely distributed in the Philippines, in open moist areas, open grasslands and open thickets
Constituents• Seed contains a toxic alkaloid, mimosine, a
non-protein alpha-amino acid, known to cause hair loss and depressed growth in mammals
• Roots yield flavonoids, phytosterol, alkaloids, amino acids, tannins, glycoside, fatty acids.
• Leaf extract have yielded an adrenaline-like substance.Seeds contain a mucilage composed of d-xylose and d-glucoronic acid, yielding 17% greenish yellow fatty oil.
Medicinal Properties
• Asthma; expectorant
• Antidepressant
• Root is aphrodisiac
• Analgesic
• Antispasmodic
• Leaves are used for diabetes
• In India, it is used as antifertility agent
• Mild sedative property
• Sedative drugs reduces anxiety (anxiolytic) and exert calming effect.
• Acts like Benzodiazepines, Barbiturates (Sedative- Hypnotic drugs)
NEUROPHARMACOLOGY
• GABA- major inhibitory neurotransmitter
• Potentiate GABAergic inhibition at all levels of the neuraxis, enhance GABA’s effects by increasing the frequency of channel-opening events ( Cl ion channels)
Effects of Sedative- Hypnotic Drugs
• Sedative drugs accompanied by depressant effects on cognitive and psychomotor functions
• inhibiting the development and spread of epileptiform electrical activity in the central nervous system (Anti- convulsant)
• Muscle relaxant- anti- spasmodic
• Conclusion
Makahiya is a delayed long-acting depressant effect
Causing mild sedation and anti- depressant.
Expt. E: HMP ON BLOOD SUGAR
• 10% Glucose- SD
• R.D 0.1mL
• Animal: albino mice
Procedure
• Starved and acclimatized the mice. Do not stress the mice
• Determine the base line blood glucose (Glucometer method)
• Inject 0.1ml 10% glucose intraperitonealy after 15min.
• Gather the blood glucose after 30min every 15min for 60min.
Result
Baseline 15 mins after
30 mins. After
45 mins. After
60 mins. after
0.3 mmol/L 0.6 mmol/L
2.5 mmol/L
2.0 mmol/L
2.5 mmol/L
Glucose
• It is a monosaccharide, and is the most important carbohydrate in biology as a source of energy and metabolic intermediate
• Glucose is an important metabolic intermediate of energy.
• It is the treatment for hypoglycemia, or low blood sugar level, by providing the glucose needed in the blood.
• Conclusion
Glucose has a sustained short- acting Hyperglycemic effects
10% Glucose- Hypertonic solution
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