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5/9/2017

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Histology Utilization in Biomedical ResearchGrace Jenson

May 5, 2017

Role of the histotechnician

• Biomedical

• Research (translation to human models)

• Direct involvement in sample collection and discussion• Identification of appropriate histological methods

Review of regenerative medicine

• Three interrelated approaches:• Rejuvenation

• Replacement

• Regeneration

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Stem cell 101

• Stem cell basics

• Embryonic vs adult stem cells

• Induced pluripotent stem cells

Stem Cells 101

https://www.youtube.com/watch?v=aAGaPH3s74g#action=share

https://www.rndsystems.com/resources/articles/differentiation-potential-induced-pluripotent-stem-cells

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Examples in application

• Wound healing• Matrix (tissue engineering)• Processing and fixation• Staining

• Sucrose gradient processing• Endogenous fluorescent tags

• Trace MRNA-lineage tracing

• Whole organ review (mega block)

• Electron microscopy• Resin embedded samples


• Wound healing• Matrix (tissue engineering)

• Processing and fixation

• Staining


• Sucrose gradient processing• Endogenous fluorescent tags

• Trace MRNA-lineage tracing

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http://www.cosmobio.co.jp/export_e/products/detail/products_sge_20070824.asp?entry_id=3423

https://www.nature.com/articles/ncomms12260


• Whole organ review (mega block)• Sliding microtome

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• Electron microscopy• Resin embedded samples

• Ultra structure

http://www.izw-berlin.de/electron-microscopy.html

Current and Future Trends

• Digital pathology

• Immunohistochemistry• Layered staining

• Modern fluorescent staining• Infrared/photon

• Mega blocks

Immunohistochemistry staining

• Triple staining/ multiple color fluorescence (current)• Alexa Fluor

• Infrared (LICOR) (future)• Proton emission

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http://circinterventions.ahajournals.org/content/7/4/560.figures-only

https://www.licor.com/bio/applications/tissue_sections/


• Triple staining/ multiple color fluorescence (current)

• Alexa Fluor

http://www.biocompare.com/9776-Antibodies/4478620-Alexa-Fluor-Labeled-Secondary-Antibodies-and-Streptavidins/

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• Infrared (LI-COR) (future)• Proton emission

https://www.google.com/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjuwf-wk87TAhWD14MKHbu_DncQjhwIBQ&url=http%3A%2F%2Fwww.licor.com%2Fbio%2Fblog%2Freagents%2Firdye-reagents-reagents%2Fmicroscopy-with-near-infrared-fluorescence-for-outstanding-images%2Fattachment%2Fmicroscopy_img_d-2&psig=AFQjCNE7cQGt3sonvbBUbj2ZXvpzRWoqMg&ust=1493709226783553

Needs of research

• Whole organ review• Elimination of image stitching

• Efficiency and cost• Multiple staining

• Co-localization

• Limited samples/resources

• Vibratome• Neurology

• Versatility

• Troubleshooting

The ultimate goal of regenerative medicine

Healing from Within: The Promise of Regenerative Medicine

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Contact Information

• LinkedIn

• [email protected]

References

Kang, J. Y. & Zheng, L. (2013). Rejuvenation: an integrated approach to regenerative medicine. Regenerative Medicine Research. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4376090/pdf/40340_2013_Article_6.pdf

National Institutes of Health. (2016). Stem Cell Information. Retrieved from https://stemcells.nih.gov/info/basics/1.htm

Rivera, E.A. & Jayo, M. J. (2013). Histological evaluation of tissue regeneration using biodegradable scaffold seeded by autologous cells for tubular/hollow organ application. Methods in Molecular Biology. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/23494443

R & D Systems. (2017). Differentiation potential of induced pluripotent stem cells. R & D Systems: A Biotechne Brand. Retrieved from https://www.rndsystems.com/resources/articles/differentiation- potential-induced-pluripotent-stem-cells