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Development of Xanthone
Powder Production Technology
Wilaisri Limphapayom
Post harvest and Products Processing
Research and Development Division
Department of Agriculture.
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Mangosteen Fruit
Garcinia mangostana Linn.
is tropical fruit available in Thailand.
Nutraceutical mangosteen
composition
is mangosteen fruit pulp and rind.
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Extracts from Mangosteen Rind
The extracts from mangosteen rind mainly
compose of xanthone , anthocyanins and
tannins.
Xanthone group in mangosteen extractscompose of alpha-mangostin (1,3,6-
trihydroxy-7-methoxy-2,8-bis-(3-methyl-
but-2-enyl)-xanthen-9-one) and gamma-mangostin (1,3,6-trihydroxy-2,8-bis-(3-
methyl-but-2-enyl)-xanthen-9-one). And
others.
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Bioactivities of Xanthone.
The xanthone has demonstrated
antioxidant,
antibacterial,anti-inflammatory activities and
the ability to induce cancer cell.
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Objective
The objective of this research is to
Extraction of xanthone frommangosteen rind by using solvent extraction.
Preparation of mangosteen powder
Utilization of Mangosteen rind extract.
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Material and Method
Extraction of Mangosteen rind.
1. Dried and Powder mangosteen rind.
2. Solvent Extraction by Ethanol, Methanol.
3. Soxlet Extraction./Continuous Extraction.
4. Rotary Evaporator
5. Dried in vacumm oven
6. Accurated weight.
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Extraction of Mangosteen rind.
600 gram of dried mangosteen rind are
combined with 3000 L. of ethanol and
continueous extraction for 10 hours.
To receive crude extract by using rotaryevaporator. This method also can be with
methanol and ethyl acetate.
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Analysis of Xanthone.
Analysis of Xanthone by HPLC
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Analysis of xanthone by using LC-MS.
Analysis of alpha-mangostin, gamma-mangostin and other xanthone derivatives
have been made by using LC-MS with UV
detector , wavelength 254 nanometer. ,Hypersil ods column, 150x4.6 mm. 5µM,
at 40 C and mobile phase 0.1% (v/v)
acetic acid in water , A and 95% (v/v)methanol ,B . For mobile phase gradient
program, 65-90% B for 40 mins., rate 1 ml
per min., injection volume 10 microlitre.
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Preparation of Mangosteen juice powder.
by using spray dryer.
50 % of mangosteen juice ,
15% of maltodextrin, and
35% water are homogenized before
spraying to receive mangosteen
juice powder.
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Preparation of Xanthone Powder.
Encapsulation of xanthone
by using spray dryer.
20 % of mangostin extract ,
50% of maltodextrin, and
30% mixture of ethanol and water 1:1 are
homogenized before spraying to receive
xanthone powder.
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Result and Discussion.
Ethanol and methanol extracts are 21.75-24.33% and 20.8-22.8% of dried
mangosteen rind respectively.
Mixture of ethyl acetate and water (1:1 v/v)extracts are 7.8-10.36% of driedmangosteen rind.
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Maceration Mangosteen rind .
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Analysis of xanthone by using LC-MSshowed that one gram of dried
mangosteem rind contained alpha-
mangostin
931.79
microgram
Beta-mangosteen 19.47 microgram and
gartanins 18.7 microgram.
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One gram of methanol extracted
contained
1079.72 microgram , Beta-mangostin 21.10
microgram and gartanins 24.17
microgram.One gram of ethyl acetate extracted
contained alpha-mangostin 782.74
microgram
Beta-mangosteen
19.69
microgram and gartanins 26.04
microgram.
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Bioactivities toxicity towards cancer cells,MCF7-Breast cancer ,
IC 50 was average 17.35 microgram per ml.
Note. IC50 the amount of extract into 1 ml.solution necessary to decrease by 50%.
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Xanthone powder was manufactured fromethanol and water extract by using Spray
Dryer.
As raw material for food and cosmetics.
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Mangosteen pulb was processed to instantmangosteen powder, mixing with tea andcoffee , cookies and ice-cream.
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Conclusion
Mangosteen rind extract can be made byusing solvent extraction , such as
Ethanol and methanol by continuous
extraction or maceration.The mangosteen rind extract mainly
compose of alpha-mangostin.
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Conclusion.
Mangosteen pulp juice powder andxanthone powder can be process
by spray drying.For xanthone powder is raw
materials as food in healthy drink
and in cosmetic.
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Conclusion
Xanthone and derivatives have beenshown to have beneficial effects on
some cardiovascular diseases,
including heart disease , hypertension.The protective effect of xanthones in
the cardiovascular system may be
due to their antioxidant,
anti-inflammatory.
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Conclusion
Xanthones may also have
anti-tumor activity
thus potentially havingan influence in the prevention
or treatment ofcertain cancers.
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Conclusion
The actual active ingredients,
Xanthones are mostly in the rind and less
in the fruit.
Nutraceutical for health benefit needimproving general health and wellness in
human.
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Thank You.
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