Does Putrescine Effect on Growth, Yield and Chemical Composition of Cotton Plants Grown under Saline Soil Conditions?

Introduction

Material and Methods

Selected Reference

Aim of Study

Conclusion

The objective of this study was to investigate mitigation effect

of putrescine (Put) on growth, yield, and chemical constitutes

of Egyptian cotton (Gossypium barbadense L. cv. Giza 90)

plants under different salinity stress conditions.

-The Pots (40*40 cm) experiment was carried out in the wire

house of Fac. Agric., Cairo Uni., Giza, Egypt, during the two

successive seasons (2011 and 2012) with six replicates.

-Before sowing cotton, different concentrations (Control ‘C’ 0,

3000, 6000 and 9000ppm) of salt mixture (2 NaCl: 2 CaCl2: 1

MgSO4) were added into soil (1 clay: 1 sand) for each pot.

-Three different doses of putrescine (Control ‘C’ 0, 1 and 2

ppm) were sprayed eight times started at 45 days after planting

(DAP) (29thMarch) and repeated every 15 days.

-Samples were taken at 100 DAP in two seasons, and the yield

after 210 & 230 DAP (2 picks) were taken.

-Determinations of K, Proline were carried out on the dry

material and total Chlorophylls on fresh material.

Spraying putrescine 2 ppm was significantly increased the

growth characters and yield of cotton plants under the absence

or presence of different soil salinity levels.

Gill, S.S. and Tuteja, N. (2010). Polyamines and abiotic stress

tolerance in plants. Plant Signaling & Behavior, 5(1): 26–33.

Results

The results indicated that there is an decrease in leaf area per

plant, shoot dry weight, K, and Total chlorophylls, while proline

was increased, but spraying of Put increased leaf area per

plant, shoot dry weight, K, and Total chlorophylls, while proline

was decreased under natural and salinity soil conditions as

Figures (1, 2, 3, 4, 5, 6, and 7).

A.H. Hanafy Ahmed, E. Darwish, and M.G. Alobaidy Dep. Agric. Bot., Plant Physiol. Division, Fac. Agric., Cairo Uni., Giza, Egypt

0

2

4

6

8

10

12

14

16

18

20

C 3000 6000 9000

Leaf

are

a (1

00

cm2)

Salinity levels (PPM)

Figure 1

0

2

4

6

8

10

12

14

16

18

20

C 3000 6000 9000

Sho

ot

dry

we

igh

t (g

)

Salinity levels (PPM)

Figure 2

0

2

4

6

8

10

12

14

C 3000 6000 9000

No

. of

tota

l bo

lls

Salinity levels (PPM)

Figure 3

0

0.5

1

1.5

2

2.5

3

C 3000 6000 9000

Bo

ll w

eig

ht

(g)

Salinity levels (PPM)

Figure 4

0

0.5

1

1.5

2

C 3000 6000 9000

Tota

l ch

l. (

mg

/g F

.W)

Salinity Levels (PPM)

Figure 5

0

1

2

3

4

C 3000 6000 9000K

pe

rce

nta

ge (

%)

Salinity Levels (PPM)

Figure 6

• Cotton known as the ‘King of fibre’ and called as the ‘White

Gold’ is a major world fibre crop. In past it was accounted as

main source of Egyptian economy.

• Salinity inhibits plant growth and productivity.

• Evidences showed that polyamines are involved in many

physiological processes of plants, such as cell growth and

development and respond to stress tolerance to various

environmental factors (Gill and Tuteja, 2010).

0

0.5

1

1.5

2

C 3000 6000 9000

Pro

line

(m

g/g

D.W

)

Salinity Levels (PPM)

Figure 7

C: 0 PPM

Put 1 PPM

Put 2 PPM