http://www.discoverlife.org/20/q?search=Ambystoma+tigrinum
Cd~0.2 mg/Kg
CdBelow detection limit
metamorphosis
paedomorphosis
http://www.disk-world.net/nucleus/media/1/LandUse_LC.jpg
NDSU Agriculture Research Center, Minot, Summer 2006
MS222, 10% Formalin,70% Ethanol
Dissection, stomach removal, and content analysis
Larval V.S. Transformed – Significant Difference in Stomach mass and Content
Male V.S. Female – No significant difference
Juvenile V.S. Adult – No significant difference
Head width Stomach Content Mass Snout Vent Length (SVL)
What They What They EatEat
Food Profile for Larval Ambystoma tigrinum
Food Profile for Transformed Ambystoma tigrinum
Food Profile for Larval Ambystoma tigrinum
61.4%
•~ 400 known species•0.5-3 mm in length•Live in nearly all inland freshwater bodies•Feed on organic material found in sediment particles
R. Brusca &G. Brusca, Invertebrates, second edition, 2003, Sinauer Associates, Inc pp. 522
http://www.microscope-microscope.org/gallery/Mark-Simmons/pages/Cladoceran.htm
Food Profile for Larval Ambystoma tigrinum
Food Profile for Transformed Ambystoma tigrinum
2.7%
37.2%
•~ 25,000 known species.•Most are parasitic but some are free-living.•Live in marine, freshwater and terrestrial environments.•Species found were ~15-30 mm in length•The free living forms feed on the fungi and bacterial
R. Brusca &G. Brusca, Invertebrates, second edition, 2003, Sinauer Associates, Inc pp. 351-362
Food Profile for Larval Ambystoma tigrinum
Food Profile for Transformed Ambystoma tigrinum
15.8%
6.2%
•Live throughout North America and most commonly found in cool and clear streams. However, is not the case for us, our individuals were in a pond environment.•11-23 mm in length•Filter feeders, feeding fungi and bacteria.
J. Voshell, Jr., A Guide to Common Freshwater Invertebrates of North America, 2002, The McDonald & Woodward Publishing Company pp. 382-383
Food Profile for Larval Ambystoma tigrinum
Food Profile for Transformed Ambystoma tigrinum
18.5%
5.6%
•Live throughout North America in light flowing freshwater. •25-50 mm in length•Predatory feeders.J. Voshell, Jr., A Guide to Common Freshwater Invertebrates of North America, 2002, The McDonald & Woodward Publishing Company pp. 305
•Live throughout North America in freshwater in ponds, lakes, marshes, and diches.•30-40 mm in length•Predatory feeders.J. Voshell, Jr., A Guide to Common Freshwater Invertebrates of North America, 2002, The McDonald & Woodward Publishing Company pp. 301-302
Identify Nematode species Analyze individuals from other ponds
Stomach content of terrestrial individuals
Adequate pond samples
ACKNOWLEDGMENTS
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