Daria Bedulina
Laboratory of biosystem adaptation Irkutsk State University
Adviser: Dr M. Timofeev
Modification of MXR-activity in freshwater amphipods Eulimnogammarus cyaneus
and E. verrucosus by organic and inorganic contaminants.
Organism
STRESSADAPTATION
The organisms stress adaptation levels:
1. Behavioral2. Physiological3. Biochemical and molecular
Multixenobiotic resistance system (MXR)
P-glycoprotein(P-gp)
Multixenobiotic resistance system (MXR)
Method of MXR-activity definition:
Rhodamine B efflux method
LAKE BAIKAL
Lake Baikal
Amphipods (Crustacea, Amphipoda) More than 250 species and subspecies
Fauna >> 2500 species (60-80% endemics)
Contaminants, used in research
Organic contaminant Natural organic matter (NOM)
Inorganic contaminant CdCl2
Humic substances
22
The objects of research:
Eulimnogammarus verrucosus (Gerstf.)
E.cyaneus (Dyb.)
P-gp
Rhodamine B-efflux method
Accumulation
P-gp
Rhodamine B-efflux method
Efflux
MXR-activity in amphipodsMXR-activity in amphipods
without contaminants (control)
(Rhodamine B efflux ))
Eulimnogammarus cyaneus Eulimnogammarus verrucosus
Results
MXR-activity in amphipods, MXR-activity in amphipods,
NOM solutions (60 mg/ml)
(Rhodamine B efflux ))
Eulimnogammarus cyaneus Eulimnogammarus verrucosus
control
NOM
Results
Eulimnogammarus cyaneus Eulimnogammarus verrucosus
control
CdCl2
Results
Discussion
1. Baikalian amphipods use a MXR system for xenobiotics efflux.
2. Activity of this system is inhibited by some organic and inorganic contaminants.
Conclusion
The multixenobiotic resistance mechanism in baikalian organisms is sensitive to organic and inorganic contaminants, such as heavy metal and NOM.
This research was partially supported by grants of Russian Foundation for Basic Research
No. 05-04-97239-Baikal, 05-04-97263-Baikal
and 06-04-48099
The presented results was published
Timofeyev M., Shatilina Z.M., Bedulina D.S., Menzel, R., Steinberg C.E.W. Natural organic matter (NOM) has the potential to modify the multixenobiotic resistance (MXR) activity in freshwater amphipods Eulimnogammarus cyaneus (Dyb) and Eulimnogammarus verrucosus (Gerst.)// Comparative biochemistry and physiology, Part B 146 (2007) 496–503
Thank you!
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