APPLICATION OF RADIATION PRODUCED OLIGO CHITOSAN AS PLANT GROWTH PROMOTER IN
INDONESIA BY FIELD TEST
DR IR GATOT TRIMULYADI REKSOE-mail: [email protected]
eNATIONAL NUCLEAR ENERGY AGENCY
I N D O N E S I A
INTRODUCTION Indonesia is the world's largest archipelago with around 13,000 islands, of which 6.000 are
inhabited, and occupies a 5,100-km stretch from the Indian to the Pacific Ocean.
Irradiation Facilities
• Latex iradiator : 135 kCi (Okt 2008)• MultiPurpose Panoramic irradiator : 8.5 kCi• Gamma Chamber 4000A : 1.26 kCi• Gamma Cell-220 : 51 Ci• Electron Beam Machine : 300 keV/50 mA• Electron Beam Machine GJ-2 : 2 MeV/10 mA
Latex Irradiator, the Control Panel
Electron Beam Machine Specifications No EPS-300 Type GJ-2 Type
1 Installation year 1984 1994
2 Origin Nissin High Voltage, Japan
Shanghai Xian-Feng Electrical Manufacturing Work, China
3 Type Cockroft-
Walton Dynamitron
4 Max tension 300 kV 2000 kV
5 Max energy 300 keV 2000 keV
6 Max flow 50 mA 10 mA
7 Sample transportation
Konveyor Konveyor
Electron Beam Machine GJ-2, 2 MeV- 10 mA
WHY RADIATION TECHNOLOGY IS WHY RADIATION TECHNOLOGY IS IMPORTANT ?IMPORTANT ?
Address all aspects of Millennium Project !!!
HealthHealth EnvironmentEnvironment SafetySafety New materialsNew materials
Shell seafood waste such as shrimps shell and craps are abundantly in Inonesia
Shrimps shell Craps shell
The remarkable rapid and successful expansion of shrimps processing industry in Indonesia suggests the possibility of utilizing shrimps-processing waste as raw material for the manufacture of many valuable products such as chitin and chitosan for industrial and health care product
Indonesian shrimps production in 2006 : 350,000 tons
Chitosan is a linier polysaccharide derived from chitin A mayor component of the shell of the crustacean organisms The second most abundant biopolymer in nature next to cellulose.
In the last year chitosan has proved to be valuable product for using in different application such as seed coating, chelating and growth promoters etc
Chitosan reported to have various biological functions, for instance, anti microbial activity, growth inhibitor of some pathogens
DEACETY-LATION
CHITOSAN
SHRIMPS/CRAB SHELL
DEPROTEINATION
DEMINERALIZATION
CHITIN
NaOH, 1M
HCl, 1M
WASTE
WASTE
liquid Fertilizer
Preparation of chitosan
Chitosan
WASTE FROM CHITIN PROSES :
LIQUID ORGANIC FERTILIZER
No
Parameter
Results
Unit
Technique Analysis
1
2
3
4
5
6
7
8
C- Organic
N- Element
- N- organic
- N-NH4
- N-NO3
Suspended solid
Heavy metal ions
-As
- Hg
- Pb
- Cd
pH
Totally of
P2O5
K2O
Pathogen microbe
- E. Coli
- Salmonella sp
Micro elements (%)
- Zn
- Cu
- Mn
- Co
- B
5.12
0.63
0.081
0.054
0.8
0
0
0.86
0.13
7.5
4.57
4.62
0
0
1.34
11.32
2.09
3.43
6.12
%
%
%
ppm
%
cell/g
ppm
Wet Oxidation
Kjeldahl,Titrimetry
Screening
AOAC 973.03.2000
A A S
AOAC 986.15,2000
AOAC 971.21,2000
AOAC 999.10,2000
AOAC 957.02.2000
pH meter
Spectrometry
AOAC 958.01,2000
AOAC 983.02.2000
Most Probable Number (MPN)
A A S
AOAC 975.02,2000
AOAC 975.01,2000
AOAC 972.03,2000
EWW 311 B,1988
AOAC 982.01,2000
A A S : Atomic Absorption Spectrophotometry
AOAC : Analysis of analytical Chemis
EWW : Examination of water and wastewater
. Chemical analysis of liquid waste from chitin process
PolysaccharidesPolysaccharidesChitin/Chitosan, Chitin/Chitosan,
and their derivativesand their derivatives
PolysaccharidesPolysaccharidesChitin/Chitosan, Chitin/Chitosan,
and their derivativesand their derivatives
DegradationDegradation
・ Solid stateDilute aq. solution
CrosslinkingCrosslinking
Irradiation
Carboxymethylchitin Carboxymethylchitosan
ApplicationsIn Agriculture, Industry,
Food, Medicine, Cosmetic Fields
MODIFICATION OF CHITIN-CHITOSAN BY IRRADIATION TECHNIQUE
Radiation processing of natural polysaccharides:• Degradation to regulate low down the molecular weight• Production of oligosaccharides• Crosslinking of polysaccharide derivatives (CMC)• Grafting monomer onto polysaccharides & their
derivatives
Natural polysaccharides (Chitin/chitosan):• Largely available quantity in nature• Recyclable biopolymers• Potential starting materials for radiation treatment on
industrial scale
PRODUCTION OF OLIGO CHITOSAN
- LACTIC ACID IRRADIATION - WATER
CHITOSAN
OLIGO CHITOSAN
MIXING VESSEL
IRADIATION TANK
The IRKA irradiator facility at PATIR-BATAN could be used for production of oligo chitosan with capasity of 1000 L/batch
For plant growth promotors
MIXING PROCESS Chitosan lactic acid NaOH 2M Demineralized
water
IRRADIATED CHITOSAN AS GROWTH PROMOTERS
CHITOSAN OLIGO -CHITOSAN
IRRADIATION
Function :
• To rise immunity and power re
distance of the plants
• To enrich the cell protein, boost
the metabolism of the plants
• To protect the surface of the
plants, repress the diseases
• To supply the utilization of been
official microbe, conduce the
procreative power
• To conduce photosynthesis, en
hance the quality and output
• To gives plant roots with new
vigor and increase the power of
EFFECT OF IRRADIATED CHITOSAN TO THE EFFECT OF IRRADIATED CHITOSAN TO THE ROOT OF THE PLANTROOT OF THE PLANT
0 kGy 25 kGy 50 kGy 100 kGy
Field test of some plants
Some field test for chili, potato and carrot plant were done, to know the persentage of increasing the crops of the haverst using irradiated chitosan plus waste of chitin process compare with ussualy fertilizer used by the farmers
Before the seed put in the soil, the soil were spray by waste of chitin process and continue combine with oligo chitosan until the plant is grow up, it’s taken around 2-3 month. After that only irradiated chitosan until get the harvest
For application waste of chitin was dilution by water 1 : 40, and spray it to the soil, and oligo chitosan dilution with water to get 50 ppm and spray to the plants
1. Chili plants
Preparation of the plots for the experiment of red chili plants on the field
Field test of oligo chitosan on yield of red chiliField test of oligo chitosan on yield of red chili
Control + Oligo Chitosan
Control + Oligo Chitosan
Potato plant
Carrot plant
cooperation
FARMER ASSOCIATION UNDER GUIDE LINE OF BATAN
SOCIALITATION
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