Chill Coma Recovery Time of Hsp70Ba Deletion Drosophila
melanogaster Mutant Compared to Drosophila melanogaster
Joe Geiger and Amit Telwala
Introduction• Background Information• Question: How does the deletion of the
Hsp70Ba gene affect the chill coma recovery time of the mutant compared to D. melanogaster?
• Hypothesis: Due to the deletion of the Hsp70Ba gene, the chill coma recovery time will be much longer for the selected mutant compared to D. melanogaster.
• Null Hypothesis: There will be no difference in the chill coma recovery of the selected Hsp70Ba deletion mutant and D. melanogaster.
Experimental Design• Drosophila melanogaster and
Hsp70Ba deletion Drosophila melanogaster mutant
• D. melanogaster will serve as the control
• 3 vials each containing 10 flies placed into Styrofoam apparatus
• Four trials– 2 for wild-type and 2 for mutant separated by sex
Materials
Experimental Design• The flies (3 vials)
were placed in 4°C for 1 hour and removed to room temperature and video recorded for recovery time
• The results analyzed using paired t-tests through the Statistical Packaging for the Social Sciences (SPSS)
Methods
Results
Results
Results
Results
Discussion• Significant Difference between Chill Coma
Recovery Time of D. melanogaster vs. Hsp70Ba mt
• Confirms from Previous Research: Heat Shock
Protein 70Ba directly affects ability to respond in a
stressful environment
• Unexpected: Trial for Female D. melanogaster
Temperature at 4.5°C
Conclusion• Reject the null hypothesis• Conclude that the Hsp70Ba gene
affects the ability of D. melanogaster for chill coma recovery time
• Future Research: Whether or not the expression of Hsp70 genes are different in flies natural global regions
• Future Research: Working with mutants that have an overexpression of Hsp70Ba to observe chill coma recovery time
References ANDERSON, A. R., HOFFMANN, A. A., & McKECHNIE, S. W. (2005). Response to selection for rapid chill-coma recovery in drosophila melanogaster: Physiology and life-history traits. Genetical Research, 85(1), 15-22.
Macdonald, S.s, L. Rako, P. Batterham, and A.a Hoffmann. "Dissecting Chill Coma Recovery as a Measure of Cold Resistance: Evidence for a Biphasic Response in Drosophila Melanogaster." Journal of Insect Physiology 50.8 (2004): 695-700. Web.
Mockett, Robin J., and Matsumoto, Yuri. “Effect of Prolonged Coldness On Survival and Fertility of Drosophila Melanogaster.” Plos One 9.3 (2014): 1-15. 2014.
ANDERSON, A. R., HOFFMANN, A. A., & McKECHNIE, S. W. (2005). Response to selection for rapid chill-coma recovery in drosophila melanogaster: Physiology and life-history traits. Genetical Research, 85(1), 15-22.
Takahashi, Kazuo H., Phillip J. Daborn, Ary A. Hoffmann, and Toshiyuki Takano-Shimizu. "Environmental Stress-Dependent Effects of Deletions Encompassing Hsp70Ba on Canalization and Quantitative Trait Asymmetry in Drosophila Melanogaster." Ed. Michael Hendricks. PLoS ONE 6.4 (2011): E17295. Web.
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