DNA base composition ribosomal DNA (rDNA) sequencing DNA
fingerprinting DNA-DNA hybridization classification &
identification: genetic analysis
Slide 8
classification: hydrogen bonds & %GC experimentally derived
1.denature DNA 2.read abs. @ 260nm 3.abs of 1 >> 2 from
DNA/RNA sequence
Slide 9
classification: NA sequencing
Slide 10
Identification & classification: sequencing
Slide 11
identification: RFLPs
Slide 12
classification: nucleic acid hybridization
Slide 13
MethodClassificationIdentification morphologynoyes stainingyes
(Gram)yes biochemical testingnoyes serologynoyes flow
cytometrynoyes NA hybridizationyes(yes) %GCyesno DNA
fingerprintingnoyes rRNA/rDNA sequencingyes
Slide 14
Chapter 10 Learning Objectives 1.Define and differentiate
taxonomy, phylogeny, identification and classification.
2.Categorize each of the following in terms of the classification
and identification of bacteria: morphology, differential staining,
biochemical testing, western blot, serology, ELISA, flow cytometry,
DNA fingerprinting, %GC analysis, rDNA (rRNA) sequencing, DNA-DNA
hybridization. Know why each does or doesnt work for classification
and/or identification. 3.If given the percent similarities for a
group of organisms and a blank phylogenetic tree, be able to place
the 4 organisms appropriately onto the tree. 4.How has rDNA
sequencing and the work of Carl Woese changed the way organisms are
categorized based on their similarities? 5.How do RFLPs allow for
the identification of unknown bacteria?
microbial diversity habitat variety ___________________
_________________________________________________________
metabolize highly unique substances integral to many
___________________ cycles nutrient fixing into organic molecules
in situ PCR >billions of bacteria/gm of soil 30-50% of aquatic
plankton are Archaea 5000 non-eukaryotic formally described (cf.
1/2 million insects)
Slide 19
Chapter 11 Learning Objectives 1.What are the general
characteristics shared by all prokaryotes? 2.In general, what can
be said about the habitats of Archaea? 3.What roles do prokaryotes
play in the environment? Where do they live, what do they
contribute to the biosphere? 4.What has in situ PCR told us about
microbial diversity?