CELL TECHNOLOGY FOR CELL PRODUCTS
Cell Technology for CellProductsProceedings of the 19th ESACT Meeting,Harrogate, UK, June 5-8, 2005
Edited by
Rodney SmithCTM BioTech Ltd, Babraham Cambridge, UK
A C.I.P. Catalogue record for this book is available from the Library of Congress.
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Contents 19th ESACT Meeting Committees
Acknowledgements
CHAPTER I: PERSPECTIVES OF THE BIOTECH INDUSTRY
Tony Bradshaw CHAPTER II: TRANSCRIPTION TO SECRETION A Novel AIR-induced Gene Expression System in HEK.EBNA Cells and its Applications 11–18 Towards Stronger Gene Expression - a Promoter's Tale 19–28
Improving the Expression of a Soluble Receptor: Fc fusion Protein in CHO Cells by Coexpression with the Receptor Ligand 29–39
Growth, Apoptosis and Functional Genomics Analysis of
Microarray Analysis of Metabolically Engineered NS0 Cell Lines Producing Chimeric Antibody 47–52
List of Participants xxxiii
xxiii ESACT Executive Committee xxv Sponsors xxvii Companies in Trade Exhibition xxix
xxxi
v
N.S. Werner, W. Weber, M. Fussenegger, and S. Geisse
G.W. Lee, J.K. Fecko, A. Yen, D. Donaldson,
S. Kalwy, J. Rance, A. Norman,
CHO-K1 Over-Expressing Telomerase 41–45
and M. Leonard
and R. Gay
G. Khoo, F. Falciani, and M. Al-Rubeai
and M. Al-Rubeai F. Crea, D. Sarti, F. Falciani,
C. Wood, S. Tobler, S. Vunuum, Y. Luo,
The UK bioprocessing sector 3–7
vi
Optimizing Medium for Transient Transfection 53–55
Establishment of Recombinant CHO Cell Lines Under Serum-free Conditions 57–61
63–65
Characterisation of the Induction of Cytochrome p450 Enzymes in Primary Cultures of Human Hepatocytes 67–70
71–85
87–89 T. Hamasaki, S. Aramaki, T. Imada, K. Teruya, S. Kabayama,
Molecular Mechanism of TAK1-Induced Repression of hTERT Transcription 91–93
Information on High Producing Recombinant CHO Cell Lines 95–98
Redox Regulation by Reduced Waters as Active Hydrogen
S. Shirahata, Y. Li, T. Hamasaki, Z. Gadek, K. Teruya,
Novel Cell-based Assay for the Detection of Murine type I IfN
M. Maura, Y. Katakura, T. Miura, T. Fujiki,
type 2 Diabetes 99–101 Donors and Intracellular ROS Scavengers for Prevention of
Z.-H. Geng, W. Nudson, L. Davis, S. Luo, and K. Etchberger
M. Kuchenbecker, B. Rehberger, M. Schriek, J. Gackowski, and R. Fehrenbach
M. Bollati-Fogolín, and W. Müller
beta-Interferon in CHO Cells M. Spearman, J. Rodriguez, N. Huzel,
Effect of Culture Conditions on Glycosylation of Recombinant
K. Sunley, and M. Butler
Y. Katakura, K. Otubo, S. Morisawa, and S. Shirahata
H. Shiraishi, and S. Shirahata
C. Lattenmayer, M. Loeschel, K. Schriebl, E. Trummer,
Analysis of Genetic Parameters in Order to Get More
K. Vorauer-Uhl, D. Mueller, H. Katinger, and R. Kunert
S. Kabayama, K. Otsubo, S. Morisawa, Y. Ishii, and Y. Katakura
Reduced Water/platinum Nanoparticles in Cancer Cells
Contents
T.L. Freeman, A.P. Chadwick, M.S. Lennard,
Induction of Caspase-3-dependent Apoptosis by Electrolyzed
C.M. Martin, R.G. Turcan, and D. Blond
vii
Transcription Profiling of Different Recombinant CHO Clones Based on Cross-species Microarray Analysis 103–106
Adaptation without Cell Division? 107–110
Release of Plasmid-DNC from PEI/DNA Particles 111–115
Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody 117–119
Design Approach Regarding Humanization and
121–123
The Role of the Sumoylation Pathway in Transient and Stable Recombinant Protein Expression in Chinese Hamster Ovary Cells 129–132
Effect of Different Materials Used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium 133–136
Antibody Production by GS-CHO Cell Lines Over Extended Culture Periods 137–140
A.J. Porter, A.J. Dickson, L.M. Barnes, and A.J. Racher
E. Trummer, W. Ernst, D. Müller, K. Schriebl, C. Lattenmayer, K. Vorauer-Uhl, R. Kunert, H. Katinger, and F. Hesse
K.L. Astley and M. Al-Rubeai
M. Bertschinger, A. Schertenleib, G. Backliwal, M. Jordan, and F.M. Wurm
S. Matsumoto, Y. Katakura, M. Yamashita,
Functionality of an Anti-CD18 Monoclonal Antibody
Y. Aiba, K. Teruya, and S. Shirahata
C.A. Caldas, D.P. Souza, M.T.A. Rodrigues, A.Q. Maranhão,
for the Production of the Cancer Testis Antigen NY-ESO-1 125 128
A.M. Moro, and M.M. Brigido
D.L. Hacker, L. Cohen, N. Muller, H.-P. Thanh, E. Derow, G. Ritter,
Development of a Transient Mammalian Expression Process
and F.M. Wurm
M. Bertschinger, D. Hacker, and F. Wurm
P. Aizawa, G. Karlsson, C. Benemar, V. Segl, K. Martinelle, and E. Lindner
–
Contents
viii
Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells 141–146
Effect of the Scaffold in Maintenance of Suitable Chondrcyte Morphology in Cartilage Regeneration 147–151
Metabolic Changes Occuring During Adaptation to Serum Free Media 153–156 The Expression of the neo gene in GS-NS0 Cells Increases their Proliferative Capacity 157–162
CHAPTER III: THERAPEUTIC CELL ENGINEERING The Therapeutic Potential of Human Embryonic Stem Cells 165–184
Investigations of Murine Embryonic Stem Cell Maintenance by Analyses of Culture Variables and Gene Expression 185–189
New Electrofusion Devices for the Improved Generation of Dendritic Cell-tumour Cell Hybrids 207–216
J.J. Cairó, and F. Gòdia
S.H. Cedar, M.J. Patel, S.J. Pickering, P.R. Braude, and S.L. Minger
M. Schomberg, C. Hakemeyer, H. Büntemeyer,
J. Rodríguez, X. Rubiralta, J. Farré, P.J. Fabregues,
L. Stiens, and J. Lehmann
F. Veraitch and M. Al-Rubeai
S. Denby, F. Baganz, and D. Mousdale
J.M. Piret, C.H. Glover, M. Marin,
Generation of Cardiomyocytes Derived from Mouse
M.A.S. Chaudhry, C.J. Eaves, B.D. Bowen,
ESC in a Scale-up System 191–195
Expansion of Undifferentiated Human Embryonic Stem Cells 197–205
S. Niebruegge, H. Ebelt, M. Jungblut, H. Baer, M. Schroeder,
R.K. Humphries, C.D. Helgason, and J. Bryan
R. Zweigerdt, T. Braun, and J. Lehmann
A.B.H. Choo, J. Crook, and S.K.W. Oh
J. Schaper, H.R. Bohnenkamp, and T. Noll
J.M. Aguilera, J. Barrachina, F. Granell, J. Nebot,
Contents
ix
Influence of c-Myc and bcl-2 CO-expression on Proliferation, Apoptosis and Survival in CHO Cultures 217–220
Ex Vivo Expansion of Hematopoietic Stem Cells Using Defined Culture Media 221–224
Development of A Novel Serum-free Cryopreservative Solution 225–231
Hepatic Cell Lines CultureD on Different Scaffolds and in Different Stages for Bioartificial Liver Systems 233–238
Comparison of Flask and Bag Systems in Cultivation of Dendritic Cells for Clinical Application 239–242 L. Macke, B. Wrobel, W. Meyring, H.S.P. Garritsen,
T-Lymphocytes Transduced with SFCMM-3 Vector in a Closed System 243–250
Influence of Down-regulation of Caspase-3 and
Producing Thrombopoietin 251–258
CHAPTER IV: GENE MEDICINE
Protein Secretion From Mammalian Cells 261–268
V. Ifandi and M. Al-Rubeai
J. Hartshorn, A.S.-H. Chan, Z.-H. Geng, L. Davis, S. Luo, J. Ni, and K. Etchberger
T. Toyosawa, M. Sasaki, Y. Kato, H. Yamada, and S. Terada
K. Kaito, Y. Narita, and S. Terada
B. Wörmann, M. Rhode, W. Lindenmaier, and K.E.J. Dittmar
C. Benati, R.S. Birolo,
Apoptotic Cell Death of Chinese Hamster Ovary Cells
S.L.S. Catamancio, M. Radrizzani,
CASPASE-7 by Sirnas on Sodium Butyrate-induced
C. Sendresen, and S. Toma
Y.H. Sung, S.H. Park, S.H. Kim, and G.M. Lee
B. Stern, C. Gjerdrum, S. Knappskog, L. Minsaas, S. Nylund, L.C. Olsen, L. Olsen,
UTRtech™: Exploiting mRNA Targeting To Increase
A. Tveit, E. Vollsund, J.E. Hesketh, E. Oveland, H. Ravneberg, C. Trösse,
A. Tauler, and I.F. Pryme
Contents
x
Semi-Synthetic Mammalian Gene Regulatory Networks 269–272
Acoustic Cell Processing for Viral Transduction or Bioreactor Cell Retention 273–278
Comparison of Different Transient Retroviral Transfection Systems 279–282
CHAPTER V: CELLS TO TISSUES Artificial Human Lymphnode 285–290
Single Cell Study in a Hydrogel 291–295
Predicting Tissue Assembly of Prostate Cancer Spheroids 297–302
Cultivating Cells of Different Origin for 3d Bone Constructs
K. Suck, C. Kasper, C. Hildebrandt, S. Diederichs, M. Fischer,
Guidance on Good Cell Culture Practice 313–315
In Vitro Vascularization of Human Microtissues 317–320
V.M. Gorenflo, P. Beauchesne, V. Tayi, O. Lara, H. Drouin,
B.P. Kramer, M. Fischer, and M. Fussenegger
J.B. Ritter, V. Chow, C. Sherwood, B.D. Bowen, and J.M. Piret
and B. Opalka V. Kleff, I. Rattmann, C. Ludwig, M. Flasshove, T. Moritz,
C. Giese and U. Marx
K. O’Connor, C. Vidulescu, S. Clejan,
Considering Physiological Conditions 303–311
H. Song, and M. Venczel
T. Scheper, and M. van Griensven
S. Coecke, M. Balls, G. Bowe, J. Davis, G. Gstraunthaler, T. Hartung, R. Hay, A. Price, O.-W. Merten, W. Stokes, L. Schechtman, and G. Stacey
J.M. Kelm, C.D. Sanchez-Bustamante, and M. Fussenegger
Contents
T. Braschler, R. Johann, U. Seger, H. Van Lintel, and P. Renaud
xi
Preparation of Salmon Atelocollagen Fibrillar Gel and Its Application to the Cell Culture 321–323
Propagation of USSC for Therapeutic Approaches 325–329
Bioreactor Based Production of Functional Conditioned Medium for the Propagation of Undifferentiated Human Embryonic Stem Cells 331–334
Large-scale Expansion of Cells for Use as Therapeutic Agents 335–338
Hematopoietic Stem Cell Expansion Medium Generates a Large Population of Functional Early and Late Progenitor Cells 339–341
In Vitro Bone Formation of Multipotential Human Mesenchymal Cells in Fixed Bed Perfusion Bioreactors 343–346
Influence of Basal Media Formulations and Calcium on
Cells In Vitro 347–351
BHRF-1 as a Tool for Genetic Inhibition of Apoptotis in Hybridoma Cell Cultures 355–361
N. Nagai, S. Yunoki, T. Suzuki, Y. Satoh, K. Tajima, and M. Munekata
J. Lamlé, H. Büntemeyer, A. Reinel, T. Menne,
A. Bauwens, H. Büntemeyer, S. Terstegge, T. Nottorf,
C. van den Bos, and J. Lehmann
and M. Al-Rubeai
S.L. Leugers, D.W. Allison, Y. Liang, C. Swiderski, G. Van Zant, and L.M. Donahue
N. Zghoul, M. van Griensven, J. Zeichen,
Growth and Directed Differentiation of Human Osteogenic
I. Majore, M.M. Hosseini, H. Mayer,
CHAPTER VI: PROTEIN PRODUCTS
and V. Jäger
K.E.J. Dittmar, M. Rohde, and V. Jäger
S. Juanola, J. Vives, J. G lvez, P. Ferrer, R. Tello, J.J. Cairo, and F. G dia
O. Brüstle, and J. Lehmann
EX VIVO Expansion of CD34+ Cells in Stemline. II
J. Melero-Martin, S. Santhalingam,
TM
áò
Contents
xii
Karyotype of CHO DG44 cells 363–366 D. Martinet, M. Derouazi, N. Besuchet, M. Wicht,
Microcarrier Cultures as Sub-process 367–369
Effect of Peptones and Study of Feeding Strategies in a
Human Monoclonal Antibody 371–374
Retinoic Acid Induced Up-regulation of Monoclonal Antibody Production 375–379
Media and Cell Manipulation Approaches to the Reduction
The Production of Human Growth Hormone 385–391
Preparation of Membrane Receptor Proteins Utilising
Factory" to Finalise the Product 393–396
Silk Protein Sericin Improves Mammalian Cell Culture 397–401
Design of Culture Media for Drosophila Melanogaster
J. Beckmann, and F.M. Wurm
S. Nagel-Heyer, S. Lünse, C. Leist,
CHO Based Fed-batch Process for the Production of a
R. Böer, C. Goepfert, and R. Pörtner
V. Chotteau, C. Wåhlgren, and H. Pettersson
of Adaptation Time in GS NS0 Cell Line 381–383
and Y. Sugimoto
P. Clee and M. Al-Rubeai
F. Anton, A. Tappe, C. Kasper,
Large Scale Micro-carrier Culture and a "Pearl Freezing
A. Loa, B.-U. Wilhelm, and T. Scheper
I. Jansson, T. Fröberg, A. Danielsson, and E. Lullau
S2 Cells Producing Recombinant G Glycoprotein
M. Sasaki, and H. Yamada
from Rabies Virus 403–413
S. Terada, N. Takada, K. Itoh, T. Saitoh,
A.L.L. Galesi, F.R.X. Batista, R.Z. Mendonça, C.A. Pereira, and A.M. Moraes
From Biopsy to Cartilage-carrier-constructs by Using
Y. Inoue, H. Kawahara, S. Shirahata,
Contents
xiii
Comparison of the Cultivation of Wild and Transfected Drosophila Melanogaster S2 Cells in Different Media 415–423
Low Temperature Culture Effects on sCR1 Productivity by rCHO Cells 425–429
Proteomic Characterization of Recombinant NS0 Cell
431–434
IFN-Nucleotide and Sugar Nucleotide Contents of CHO Cells
N. Kochanowski, F. Blanchard, E. Guedon, A. Marc,
Influence of the Adaptation Procedure and the Serum-free Conditions on the N-glycosylation Pattern of EPO Produced by CHO Cells 439–442
Stability and Cytogenetic Characterization of Recombinant CHO Cell Lines Established by Microinjection and Calcium Phosphate Transfection 443–446 M. Derouazi, D. Martinet, N. Besuchet, R. Flaction,
and F.M. Wurm Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody 447–449
Design Approach Regarding Humanization and Functionality of an Anti-CD18 Monoclonal Antibody 451–454
K. Swiech, A.L.L. Galesi, A.M. Moraes, R.Z. Mendonça,
Clones Obtained by an Integrated Selection Strategy
H. Matsuoka, C. Shimizu, and T. Takeda
A. Castillo, K.R. De La Luz, S. Victores,
Cultivated in Batch Culture 435–438
R. Cacan, F. Chirat, and J.-L. Goergen
A. Marc, and J.-L. Goergen F. Le Floch, B. Tessier, J.-M. Guillaume, P. Cans,
M. Wicht, M. Bertschinger, D. Hacker, J. Beckmann,
S. Matsumoto, Y. Katakura, M. Yamashita, Y. Aiba, K. Teruya, and S. Shirahata
C.A. Caldas, D.P. Souza, M.T.A. Rodrigues,
L. Rojas, Y. Rabasa, S. Gaskell, and R. Perez
C.A. Pereira, and C.A.T. Suazo,
Glycosylation Macroheterogeneity and Intracellular
A.Q. Maranhão, A.M. Moro, and M.M. Brigido
Contents
xiv
Development of a Transient Mammalian Expression Process for the Production of the Cancer Testis Antigen NY-ESO-1 455–458
Effect of Different Materials used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium
Antibody Production by GS-CHO Cell Lines Over Extended
Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells
Effect of the Scaffold in Maintenance of Suitable Chondrcyte Morphology in Cartilage Regeneration J. Rodríguez, X. Rubiralta, J. Farré, P.J. Fabregues, J.M. Aguilera, J. Barrachina, F. Granell, J. Nebot, J.J. Cairó, and F. Gòdia Metabolic Changes Occuring During Adaptation to Serum
The Expression of the neo Gene in GS-NS0 Cells
1000 Non-instrumented Bioreactors in a Week
459–462
Culture Periods 463–466
467–472
473–477
Free Media 479–482
Increases Their Proliferative Capacity 483–488
489–495
D.L. Hacker, L. Cohen, N. Muller, H.-P. Thanh, E. Derow, G. Ritter, and F.M. Wurm
K. Martinelle, and E. Lindner
A.J. Porter, A.J. Dickson, L.M. Barnes, and A.J. Racher
M. Schomberg, C. Hakemeyer, H. Büntemeyer, L. Stiens, and J. Lehmann
S. Denby, F. Baganz, and D. Mousdale
F. Veraitch and M. Al-Rubeai
M. Stettler, M.D. Jesus, H. Ouertatani-Sakouhi, E.M. Engelhardt, N. Muller, S. Chenuet, M. Bertschinger, L. Baldi, D. Hacker, M. Jordan, and F.M. Wurm
P. Aizawa, G. Karlsson, C. Benemar, V. Segl,
Contents
xv
Optimisation of Time-space-yield for Hybridoma fed-batch
Influenza Vaccines – Challenges in Mammalian Cell
Towards pCO2 -optimised Fermentations – Reliable Sampling, pCO2 Control & Cellular Metabolism
Bioreactor and Stirred-Tank Reactor for Parvovirus
Serum-free Transient Expression of Antibodies
Monitoring Cell Physiology in Influenza Vaccine
NucleoCounter – An Efficient Technique for the Determination
On-line Biomass Monitoring in Cell Culture with Scanning
Cultures with an Adaptive Olfo-controller 497–501
509–518
Vaccine Production 519–522
Production by Flow Cytometry 531–533
of Cell Number and Viability in Animal Cell Culture Processes 535–538
Radio-frequency Impedance Spectroscopy 539–541
R. Pörtner, B. Frahm, P. Lane, A. Munack,
Culture Technology 503–508
K. Kühn, and V.C. Hass
Y. Genzel, J. Schulze-Horsel, L. Möhler, Y. Sidorenko, and U. Reichl
A. Jockwer, C. Klinger, J. Gätgens, D. Eisenkrätzer, and T. Noll
B. Hundt, S. Straube, N. Schlawin, H. Kaßner, and U. Reichl
K. Russ, P. Schlenke, H. Huchler, H. Hofer,
Using 40 Tray Cell Factories 527–530
M. Kuchenbecker, and R. Fehrenbach
J. Schulze-Horsel, Y. Genzel, and U. Reichl
Cultivation of E-FL in Perfusion Mode Using Wave®
P. Schlenke, K. Langnickel, T. Grob, F. Kohne, H. Hofer, U. Brändle, K. Russ, and R. Fehrenbach
D. Shah, P. Clee, S. Boisen, and
J.P. Carvell and C. Cannizzaro
M. Al-Rubeai
Repeated Batch Fermentation Using a Viafuge Centrifuge 523–526
Contents
xvi
The Use of a Mobile Robot for Complete Sample Management
A. Bock and U. Reichl Optimization of Culture Medium with the Use of
P. Hinterleitner, P. Ganglberger, B. Obermüller, B. Antes,
Serum-free Influenza Vaccine Production with MDCK Cells
Flow Cytometry as a Tool for Process Monitoring of
in a Cell Culture Pilot Plant 543–547
Protein Hydrolysates 553–557
559–562
563–565
567–569
Virus-cell Systems 571–573
575–577
579–581
M. Wojtczyk, R. Heidemann, K. Joeris,
Closed Loop Control of Perfusion Systems in High-density
C. Zhang, M. Burnett, A. Knoll,
Cell Culture 549–551
and K. Konstantinov
of Mab Ign 311 in Small Scale Experiments
P. Ganglberger, B. Obermüller, M. Kainer, P. Hinterleitner, O. Doblhoff, and K. Landauer
O. Doblhoff, and K. Landauer
Y. Genzel, M. Fischer, R.M. Olmer, B. Schäfer, C. Best, S. König, B. Hundt, and
Rotary Bioreactor for Recombinant Protein Production
U. Reichl
S. Navran
K.S. Sandhu and M. Al-Rubeai
B. Isailovic, R. Hicks, I.W. Taylor, A. Nienow, and C.J. Hewitt
J. Hartshorn, F. Guehenneux, K. Kolell, Z.-H. Geng, E. Atarod, T. Mohabbat, C. McCormick, P. Sutrave,
Understanding Metabolic Needs of EB14 Cells in Culture
C. Hernandez, M. Avery, S. Luo, M. Mehtali, and K. Etchberger
Optimization of a Fed-batch Strategy for Production
in Wave-bioreactor and 5L-stirred Tank Bioreactor
The Use of Multi-parameter Flow Cytometry for Characterisation of Insect Cell-baculovirus Cultures
Contents
xvii
Using Genomic Tools for the Identification of Important Signaling Pathways in Order to Facilitate Cell Culture
Plant Peptones: Nutritional Properties Sustaining
Monitoring of Extracellular TCA Cycle Intermediates
Comparison of Different Design Configurations of a 1-L Multi-bioreactor Station for Cell Culture
Ovary Cell Lines 595–597
599–601
in Mammalian Cell Culture 603–605
607–607
and Acceleratostat Experiments 609–609
Cell Culture Optimisation 611–611
Process Development 613–617
K. Kolell, J. Padilla-Zamudio, B. Schuchhardt, S. Gilliland, S. McNorton, B. Dalton, S. Luo, and K. Etchberger
D.K. Fong, S.L. Leugers, M.L. Hoepner,
N. Lin, J.L. Beckmann, J. Cresswell, J.S. Ross, B. Delong,
E. Olmos, N. Fischbach, and A. Marc
Z. Deeds, and M.V. Caple
J. Mols, N. Cavez, S. Agathos, and Y.-J. Schneider
J.B. Ritter, Y. Genzel, and U. Reichl
G. Wilson and A. Jørgensen
R.B. Nielsen and G. Wilson
G. Wilson
H. Schütte, U. Kolrep, L. Franck, and R. Wagner
and L.M. Donahue
Computational Fluid Dynamics Applied to Laboratory Scale
D.W. Allison, K.A. Aboytes, T.K. Johnson,
Cell Culture Reactors for the Prediction of Hydrodynamic Stress 587–590
A Comparison of the SixFors Fermenter System
Comparison of Cultivations of CHO Cell Line in Chemostat
and Lab Scale Fermenters For Clone Selection
Virus Production in Vero Cells Using a Serum-free Medium 583–585
Medium Development 591–593
Evaluation of a Novel Micro-bioreactor System for
Development and Application of An Animal-component
Recombinant Protein Secretion by CHO Cells
Free Single-cell Cloning Medium for Chinese Hamster
Contents
xviii
The Effect of Genetically Modified Insulin Growth Factor on Cell Proliferation and Adenovirus Productivity
for Manufacturing of Mammalian Cell Based
Purification of Rabies Virus Produced on Vero Cells Using
Human Growth Hormone (HGH) Purification From
Process Development for a Veterinary Vaccine Against
Working Towards a Chemically-defined Replacement
Effect of Vitamins or Lipids Addition on Adenovirus
Observation and Analysis of Lab Scaled Microcarrier Cultivation by In-situ Microscopy with Image
and T. Scheper
in Serum Free Medium 619–622
Biopharmaceuticals 623–628
Chromatography Techniques 629–634
Chromatographic Media 635–641
Heartwater Using Stirred Tanks 643–648
Processing Tools 657–661
A. Buckler and M. Al-Rubeai
S. Langhammer, M. Riedel, H. Bushnaq-Josting,
CHO-Cell Culture Supernatant Utilizing Macroporous
R. Brecht, R. Pörtner, and U. Marx
M. Chtioui, K. Trabelsi, and H. Kallel
J.-G. Walter, A. Tappe, C. Kasper, R. Zeidler, O.-W. Reif, and T. Scheper
I. Marcelino, M.F.Q. Sousa, A. Amaral, C. Peixoto, C. Verissimo, A. Cunha,
for Hydrolysates 649–651
M.J.T. Carrondo,
Z.W. Deeds, C.S. Updike, B.J. Cutak,
Production at High Cell Densities 653–656
and M.V. Caple
T.B. Ferreira, M.J.T. Carrondo, and P.M. Alves
G. Rudolph, A. Gierse, P. Lindner, C. Kasper, B. Hitzmann,
Evaluation of a Scalable Disposable Bioreactor System
and P. M. Alves
Contents
xix
Rapeseed Peptidic Fractions as Medium Additives in
B. Farges, B. Tessier, I. Chevalot, C. Harscoat, S. Chenu,
Influence of Rapeseed Protein Hydrolysis Conditions on Animal Cell Growth in Serum-free Media Supplemented
G. Chabanon, I. Chevalot, B. Farges, C. Harscoat, S. Chenu, J.-L. Goergen, A. Marc, and I. Marc Rapid Media and Process Optimization Using DOE:
Developing Chemically Defined Media Through DOE: Complete Optimization with Increased Protein Production
Bioreactor Process Strategies for rNS0 Cells
The Efficient Induction of Human Antibody Production in in Vitro Immunization by CPG Oilgodeoxynucleotides
Rapid Culture Media Optimization to Gain Multiple Fold Increase in Recombinant Protein Production in
Strategies for Large Scale Production
M. Pohlscheidt, U. Langer, B. Bödeker, D. Paulsen,
Proteomic Investigation of Recombinant CHO Cells
J.R. Cresswell, J.M. Zobrist, J. Wildsmith, N. Lin,
Animal Cell Bioreactors 663–665
with Hydrolysates 667–669
A Case Study 671–682
693–695
697–701
of Parapoxvirus Ovis NZ-2 707–710
in High Density Culture 711–713
I. Marc, J.L. Goergen, and A. Marc
S. Holdread, J.H. Kim, T. Oshunwusi,
in Less than 8 Months 683–691
J. Kuchibhatla, C. Hunt, and J.W. Brooks
K. Hammett, J. Kuchibhatla, C. Hunt, S. Holdread, and J.W. Brooks
D. Fernandez, J. Walowitz, W. Paul, L. Tescione, and S. Gorfien
Y. Aiba, M. Yamashita, Y. Katakura,
Two CHO Cell Lines 703–705
S.-E. Matsumoto, K. Teruya, and S. Shirahata
S.D. Storms, A. Chen, J. Yu, and T. Fletcher
H. Rübsamen-Waigmann, U. Reichl, H.J. Henzler, and H. Apeler
R. Valdes-Camin, and M. Caple
Contents
xx
Comparative Proteomic Study of Mechanisms Involved in the Expression of Recombinant Monoclonal Antibody
Monitoring Nutrient Limitations by Online Capacitance Measurements in Batch & Fed-batch CHO Fermentations
Characterisation of Shake Flasks for Cultivation of
Manufacturing of Lots of Human T-Lymphocytes
The Effects of Cell Separation with Hydrocyclones
Cultivation of Vero Cells on Microporous and Macroporous
715–721
723–726
Animal Cell Cultures 727–731
for Cell Therapy 733–737
739–744
Membrane Adsorbers 749–752
L.E. Rojas, K.R. de la Luz Hernández, S. Victores, L. Castellanos, S. Gaskell, A. Castillo, and R. Pérez
and G. Schmid S. Ansorge, G. Esteban, C. Ghommidh,
G. Jänicke, C. Sauter, R. Bux, and J. Haas
N.D. Bernardi, S. Trasciatti, A. Orlandi, F. Gaspari, I. Giuntini, T. Bacchi, S. Castiglioni,
Oxygen Control in Static Cell Cultures
E. Muru, M. Galagano, C.D. Mattei, L. Cavenaghi, and M.L. Nolli
N.D. Bernardi, E. Schwander, A. Orlandi, I. Tano, S. Castiglioni, E. Muru, F. Gaspari, M. Galgano, C.D. Mattei, L. Cavenaghi,
on the Viability of CHO Cells 745–748
and M.L. Nolli
R.C.V. Pinto, R.A. Medronho,
Erythropoietin Purification Employing Affinity
and L.R. Castilho
M.C.M. Mellado, D. Curbelo, R. Nobrega,
Microcarriers 753–755
and L.R. Castilho
M.C.O. Souza, M.S. Freire, and L.R. Castilho
in Non Amplified NS0 Myeloma Cell Line
Contents
xxi
CFD-Aided Design of Hollow Fibre Modules for Integrated Mammalian Cell Retention and Product
Comparison of Manufacture Technologies for RAV12 Monoclonal Antibody in Production Medium
A New Host Cell-line for the Fast, Reproducible and Efficient Production of Biopharmaceuticals
Analysis and Modeling of the Metabolism of Lepidopteran
Growth of Mammalian and Lepidopteran Cells on BioNOC® II Disks, a Novel Macroporous Microcarrier
Production of Human Growth Hormone in a Mammalian Cell High Density Perfusion Process T. Nottorf, W. Hoera, H. Buentemeyer, S. Siwiora-Brenke, A. Loa, and J. Lehmann
Proteomic Characterization of Recombinant NS0 Cell
Purification 757–760
761–763
769–771
Clones Obtained by an Integrated Selection Strategy 773–776
Cell Lines 777–780
781–784
789–793
R.L. Machado, A. Figueredo, D.G.P. Carneiro, L.R. Castilho, and R.A. Medronho
Biopharmaceuticals in CHO-Cells 765–767 S. Zahn, K. Abst, N. Palmen, S. Schindler, and A. Herrmann
B. Greulich, H.F. Abts, S. Zahn, and A. Herrmann
L. Rojas, Y. Rabasa, S. Gaskell, and R. Perez
J.-C. Drugmand, Y.-J. Schneider, and S. Agathos
J.-C. Drugmand, J.-F. Michiels, S. Agathos, and Y.-J. Schneider
J. Keenan, D. Pearson, and M. Clynes
Containing No Animal Derived Proteins
A. Castillo, K.R. De La Luz, S. Victores,
Screening Strategies for Iron Chelators in Serum Free Media 785–788
I. Shackel, A. Bass, A. Brewer, P. Brown,
Highly Efficient Serum-free Production of
M.C. Tsao, and L.W.S. Chang
Contents
xxii
Development of an Integrated Qualitative and Quantitative Approach to Study Expression-Secretion Bottlenecks of Mammalian cDNAs in
Rotating Bed Bioreactors: a Novel Way to Natural Proteins by Tissue-like Cultivation of Mammalian Cells
Minireactors for Animal Cell Culture: Monitoring of
Air-lift Bioreactor Improvement of Scale-up Linearity Between Shake Flasks and 25-L Scale in the Insect
HEK293 EBNA Cells 795–798
799–801
803–806
Cells–baculovirus Expression System 807–809
L. Glez, C.A. Power, T. Battle, and G. Feger
Cell Concentration and Cellular Activity
H.P.A. Hoffmeister
F. Bollin, C. Losberger, L. Chevalet,
R. Bragós, J.J. Cairó, and F. Gòdia
and T. Battle
A. Soley, J. Gálvez, E. Sarró, M. Lecina, A. Fontova,
Contents
Name Index
Subject Index
811 816 –
– 81 7 821
19th ESACT Meeting Committees
Organising Committee
Rodney Smith (Chair) CTM BioTech Ltd., UK Fiona Godsman (Trade Fair/Sponsorship) BioReliance, UK
Magellan Instruments Ltd., UK Tracey Sambrook (Social Arrangements) CTM BioTech Ltd., UK Glenda Bland (Conference Manager) Global Meeting Planning
Scientific Committee
Dr. Rod Smith (Chair) CTM BioTech Ltd., UK Prof. Mohammed Al-Rubeai University of Dublin, Ireland Prof. Dr. Martin Fussenegger ETH Zürich - Institute of Biotechnology,
SwitzerlandProf. Dr. Ing. Jürgen Lehmann Universität Bielefeld, Germany Prof. James M. Piret University of British Columbia, Canada Prof. Glyn Stacey National Institute for Biological
Standards and Control, UK Dr. David Venables BioReliance, UK
Poster Award Committee
Nathalie Chatzissavidou Biovitrum, Sweden Paula Marques Alves ITQB/IBET, Portugal
xxiii
John Bonham-Carter (Trade Fair/Sponsorship)
ESACT Executive Committee
Otto-Wilhelm Merten, Chairman Généthon III, France
Alain Bernard, Secretary Serono Biotech Center, Switzerland
Martin Fussenegger, Treasurer ETH- Hönggerberg , Zurich, Switzerland
Rodney Smith, Meeting Chair CTM BioTech, UK
Francesc Gòdia Universitat Autonoma de Barcelona, Spain
Hansjörg Hauser GBF, Germany
Stefanos Grammatikos Boehringer Ingelheim Pharma, Germany
Florian Wurm EPFL, Switzerland
Christophe Losberger, Web Master Serono Pharmaceutical, Research Institute, Switzerland
Steve Oh, Newsletter Chief Editor Bioprocessing Technology Institute, Singapore
Merlin Goldman, Newsletter Co-Editor Wyeth Biopharma, Ireland
Bryan Griffiths ESACT Secretariat, UK
xxv
Sponsors
ESACT and the Organising Committee wish to thank the following companies for their generous support.
Aber Instruments
AstraZeneca
Aventis Pasteur SA
ChemoMetic A/S
Covance
CTM BioTech Ltd.
DSM Biologics
GE Healthcare
Genetech
Genzyme Flanders NV
Invitrogen
JRH Europe
Magellan Instruments
Merck & Co., Inc., USA
Merck Research Laboratories
Rütten Engineering AG/Ltd.
Sartorius BBI Systems GmbH
Serono
Sigma Aldrich
Wave BioTech
xxvii
Companies in Trade Exhibition
Aber Instruments Ltd.
Applikon. BV/AppliSens
Areta International s.r.l.
Bayer HealthCare AG
Bayer Technology Services GmbH
BDD Diagnostic Systems Europe
Beckman Coulter
Bioengineering AG
BUA/BioProcess UK
BioProcess Engineering Services Ltd.
BioProcess International
BioProcessors Corporation
Bioquell
BioXplore
Blue Membranes GmbH
Broadley Technologies Ltd
Cambrex Bio Science
Celliance
Cellon SA
Celonic GmbH
Genetix Ltd.
Greiner Bio-One Ltd.
Guava Technologies
Helena Biosciences
Henogen
HyClone/Perbio Science
Infors UK Ltd./Hi Tec-Zang
Innovatis AG
Invitrogen
Irvine Scientific
J M Separations BV
JRH Europe
Kerry Bioscience
Lonza Biologics plc
Magellan Instruments
Moregate TCS Ltd
New Brunswick Scientific (UK) Ltd
NewLab BioQuality AG
NUNC A/S
PAA Laboratories GmbH
xxix
Companies in Trade Exhibition
Charter Medical Ltd.
Chemicon Europe
ChemoMetec A/S
Cobra BioManufacturing
Consolidated Polymer Technologies (C-Flex)
Corning Life Sciences
Covance
CTM BioTech Ltd.
DASGIP AG
Delta Biotechnology Ltd.
Eden Biodesign
European Collection of Animal Cell Cultures (ECACC)
Evotec Technologies
GE Healthcare
Genetic Engineering News
Pall Life Sciences
Panacea
Polyplus Transfection
Quest Biomedical
Rütten Engineering
Sartorius BBI Systems GmbH
Sigma Aldridch
SLS
Stedim S.A.
Tecan Deutschland GmbH
The Automation Partnership
The Williamsburg BioProcessing
Wave Biotech AG
Wave Europe Pvt. Ltd.
Xenova Biomanufacturing
YSI (UK) Limited
xxx
Acknowledgements
In the planning, organising and running the meeting a great number of people have been involved. The outcome was a successful meeting and these proceedings of the science presented. The 19th ESACT Meeting built on the success of the previous ones and lays the foundation for the 20th and beyond.
In making the meeting so successful I would like to acknowledge and thanks the following people for their effort hard work and most of all friendship over the period. These people provided immense support to me during the events and I shall never forget that.
My personal thanks must go to Fiona Godsman for staying on with the Trade Organisation and whose invaluable comments were appreciated and hopefully listened too. John Bonham-Carter (Trade Organisation) who was so keen in Tylosand to be involved and relished the chance of developing his (sometimes wild) ideas on the Harrogate meeting. Glenda Bland whose efforts in keeping us on track and focussed was extremely well received. Tracey Sambrook for her advice support and constant council on how the meeting was destined to be a success.
These people had the vision and energy to ensure that no part of the Harrogate meeting was forgotten or left out. I owe them the greatest of thanks for being part of the team.
I would also like to thanks the other people who helped in laying down the science for the community to ensure the programme was balanced and thought provoking. The main scientific committee comprising James Piret, Glynn Stacey, Martin Fussenegger, Mohammed Al-Rubeai and Jürgen Lehmann. We should not forget the organisers of the now ever present Poster award and my thanks go to Nathalie Chatissavidou and Paula Alves for the excellent organisation and selection of some truly exciting posters.
xxxi
The biggest acknowledgment and thanks must go to the delegates and all those scientists that wanted to participate in some way to the meeting. Without your contributions the meeting would not be the same.
A very big Thank you to you all.
Rodney Smith
xxxii Acknowledgements
List of Participants
Prof. Adriano Aguzzi, Institute of Neuropathology, University Hospital of Zurich, Schmelzbergstrasse 12, Zurich CH-8091, SWITZERLAND, Telephone: +4112552107, Email: [email protected]
Mr. Yoshihiro Aiba, Laboratory of Cellular Regulation Technology, Department of Genetic Resources Technology, Kyushu University, Fukuoka, JAPAN, Telephone: +84 92 642 3046, Email: [email protected]
Mr. Peter Aizawa, Octapharma AB, Nordenflychtvägen 55 Stockholm 12137, SWEDEN, Telephone: +46 8 56643436, Email: [email protected]
Mr. Franz Alig, Sartorius BBI Systems GmbH, Schwarzenberger Weg 73 - 79, Melsungen D-34212, GERMANY
Mr. Tony Allman, Infors UK Ltd., The Courtyard Business Centre, Dovers Farm, Lonesome Lane, Reigate Surrey RH2 7QT, UK
Prof. Mohammed Al-Rubeai, Department of Chemical & Biochemical Engineering, Falculty of Engineering & Architecture, University College Dublin, Belfield, Dublin 4, Ireland, Email: [email protected]
Dr. Paula Alves, ITQB/IBET, Apartado 12, OEIRAS 2781-901, PORTUGAL, Telephone: + 351 21 4469421, Email: [email protected]
Dr. Madawi Alwazzan, Flowgen Bioscience, Wilford Industrial Estate, Ruddington Lane, Nottingham NG11 7EP, UK, Telephone: +44 115 977 5494, Email: [email protected]
Dr. Hagit Amitai, Compugen Ltd., 72 Pinchas Rosen Street, Tel-Aviv 69512, ISRAEL, Telephone: +972 3 7658513, Email: [email protected]
Dr. Anette Amstrup Pedersen, Novo Nordisk A/S, Novo Alle, Bagsvared 2880, DENMARK, Telephone: +45 44 42 00 08, Email: [email protected]
Dr. Dana Andersen, Director, Late Stage Cell Culture, Genentech, Inc., 1 DNA Way - MS 32, South San Francisco, CA 94080, USA, Telephone: +1 650 225 4443, Email: [email protected]
xxxiii
List of Participants
Mr. Jorn Sondrup Andersen, Rocket Management, Ganloseparken 16, Stenlose DK-3660, DENMARK, Telephone: +45 22 16 67 26, Email: [email protected]
Mr. William Anderson, Synthecon, Inc., 8042 El Rio, Houston, TX 77054, USA, Telephone: +1 713 741 2582, Email: [email protected]
Dr. Carlo Andretta, Biospectra AG, Zuercherstrasse 137, Schlieren 8952, SWITZERLAND, Telephone: +41 44 7556700, Email: [email protected]
Mrs. Emilie Ansel, GlaxoSmithKline Biologicals S. A., rue de Institut 89, Bixensart 1330, BELGIUM, Telephone: +32 2 656 9890, Email: [email protected]
Ms. Fabienne Anton, Insitut für Technische Chemie - Universitat Hannover, Callinstrasse 3, Hannover 30167, GERMANY, Email: [email protected]
Mr. Didier Argentin, Henogen, Rue des Professeurs Jeener et Brachet 12, Charleroi B-6041, BELGIUM
Mr. Paul Armstrong, Celliance, 5655 Spalding Drive, Norcross, GA 3009, USA
Mr. William Arts, New Brunswick Scientific B.V., Kerkenbos 1101, Nijmegen 6546 BC, THE NETHERLANDS, Telephone: +31 24 371 7600, Email: [email protected]
Dr. Soheil Asgari, Blue Membranes GmbH, Rheingaustrasse 190-196, Wiesbaden D-65203, GERMANY
Ms. Kelly Astley, 41 Wealdstone Drive, Lower Gornal, Dudley, West Midlands DY3 2QR, UK, Telephone: +441384 821 487, Email: [email protected]
Mr. Alex Baker, Maverick DCM, Suite 12-16 Macquarie Chambers, 69-79 Macquarie Street, PO Box 2997, Dubbo 2831, AUSTRALIA, Telephone: +61 2 6885 1200, Email: [email protected]
Mr. Osman Bal, Git Verlag, Roessler Str. 90, Darmstadt 64283, GERMANY
Mr. John Baldwin, Rütten Engineering Ltd., Industriestrasse 9, Stäfa 8712, SWITZERLAND, Telephone: +41 1 928 29 30, Email: [email protected]
Mr. Jean-Philippe Barbe, Sanofi Pasteur, 1541 av. M. Mérieux, Marcy l'Etoile 69280, FRANCE, Telephone: +33 4 3737 3294, Email: [email protected]
Dr. Jacquiline Barnes-Locke, Helena Biosciences, UK
Dr. Barney Barnett, Biogen Idec, 5200 Research Place, San Diego 92122, USA, Telephone: +1 858 401 8650, Email: [email protected]
Mr. Timothy Barrett, University College London, UK, Email: [email protected]
Ms. Natalie Baseley, European Collection of Cell cultures (ECACC), Health Protection Agency, Porton Down, Salisbury SP4 0JG, UK
Miss. Vera Basto, ECBio-R&D in Biotechnology, SA, Ap 98, Lab 4.11, Ed ITQB, Oeiras 2781-901, PORTUGAL, Telephone: +351214469466, Email: [email protected]
xxxiv
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