An information-bearing seed for nucleating algorithmic self-assembly
Presented by : Venkata Chaitanya Goli
651366318
Robert D. Barish1, Rebecca Schulman1, Paul W. K. Rothemund, and Erik Winfree2
Outline
• Overview
• Preparation
• Experiments
• Techniques used and Results
• Principles for Success
• Future research topics
OverviewExplaining the Title
Information – bearing Seed Information in a seed molecule can determine which form is grown as well as where and when.
Self- assemblySelf-assembly creates natural mineral, chemical, and biological structures of great complexity.
This phenomena can be exploited to program the growth of complex structures, as demonstrated by the algorithmic self-assembly of DNA tiles.
Overview• What is a seed ?
Controlled growth using Nucleation Barrier
Mineral and chemical compounds Simplest form seeded growth
Multicellular development, where genomic information in the zygote directs the algorithmic construction of the entire organism
PreparationKey features to be present for a seed :
• Sufficiently well-formed • Capable of being synthesized with arbitrary information• Straightforward to implement experimentally.
high error rates, high spontaneous nucleation rates and low yields of well-formed structures.
Single-stranded DNA Finite-sized tile assemblies
Self-assembled ribbon-like crystals
incorporating significant amounts of specific information into such seeds remains prohibitive
Problems
Problems
Preparation
Each DNA tile consists of multiple strands folded into a rigid unit typically displaying 4 single-stranded ‘‘sticky ends’’ that direct the tile’s binding interactions with other tiles.
DNA Tile
Sticky ends
PreparationOrigami DNA seed Tile
Adapters
Scaffold strand 192 Staple strands32 Adapter strands
Consists
PreparationTiling theory
A tile is a geometrical shape, such as a jigsaw puzzle piece, that may be assembled with other tiles based on local matching rules.
Problems :
• Go beyond familiar fixed size
• random arrangements like fractals, binary trees, cellular automata
That’s where I come in
Preparation
The self-assembly process begins with an initial seed tile to which additional tiles can attach if they make sufficiently many
Program - The starting materials, i.e. a set of available tiles.
.
‘matching contacts’
So to summarize in simple terms
Input - The seed
Output - The structure produced by self-assembly is the output.
Experiments
Experiment 1 (Temperature-dependent ):To test the growth
Steps :1) All strands for the seed and for the tile set are
mixed together in buffer
2) Heated to 90 °C
3) Annealed slowly to room temperature.
4) The process of self assembly is observed
Experiments
At temperature 90 °C - 60 °C
• At these temperatures, the DNA double helix is unstable and dissociates into its single- stranded form
• All the stick ends are inert and don’t show any binding properties.
Experiments
At temperature 60 °C - 40 °C
• At these temperatures, the seed and individual tiles become stable because of extensive contacts between strands• Tiles do not attach to the seed
nor to each other because the two additional sticky-end contacts formed by each tile attachment are not thermodynamically favorable at these temperatures and concentrations.
Experiments
At temperature 40 °C - 20 °C
• At slightly lower temperatures, below the crystal melting temperature, slightly supersaturated conditions are achieved.• The attachment of a tile by two matching sticky ends becomes favorable, but attachment by a single matching sticky end remains unfavorable• Consequently, tiles now attach to the origami seed and then attach to each other to grow a crystal
Experiments
Experiment 2 (Width - Dependent ):
• Used a DNA tile set
Experiments
Experiment 2 (Width - Dependent ):
• This tile set is added a nucleation barrier under some controlled conditions
Experiments
Experiment 2 (Width - Dependent ):
• This tile set is added a nucleation barrier under some controlled conditions • A sample Zig-Zag pair
Experiments
Experiment 2 (Width - Dependent ):
The sequence formed from the tile formation
Experiments
Experiment 2 (Width - Dependent ):
A copy tile Set is created to maintain the bonding in a desired manner.
Experiments
Experiment 2 (Width - Dependent ):
• Additional tiles for the Copy tile set. Inset shows how correct copying of information at bit boundaries depends on preferred attachment of tiles matching two sticky ends over tiles matching just one.
1*
1*1
1
5*
5*
4
4
Experiments
Experiment 2 (Width - Dependent ):
Binary counter Tile sets
Experiments
Experiment 2 (Width - Dependent ):
• ‘‘Carry Bit 1-Block’’ and ‘‘Carry Bit 0-Block’’ are added to the Copy tile set
• The gray double tile is replaced by the beige one, whose sticky end provides the initial carry bit for each new COUNT layer.
1*
1*
1
2*Gray Tile Replacement
Observations from the experiment:
• The rate of spontaneous nucleation decreases dramatically with width, and so, when the tiles are annealed without a seed, we expect the majority of crystals to be just 4 tiles wide.
• when annealed in the presence of seeds whose adapter tiles specify a particular width ribbon, we expect crystals of the specified width will grow off the seed— even before 4-wide ribbons nucleate spontaneously.
Experiments
Observations with 8 width Origami:
Experiment 3 (Origami seed size):Magnified image of at the ribbon
Experiments
Observations with 10 width Origami:
Experiment 3 (Origami seed size):Magnified image of at the ribbon
Experiments
Observations with 12 width Origami:
Experiment 3 (Origami seed size):Magnified image of at the ribbon
Observations from the experiment:
• Within ribbons, information was copied with an error rate of 0.13% per tile.• The most significant errors were those that changed the
width of the ribbon, either by an internal lattice defect or by a double tile attaching too early, causing a premature reversal of the zig-zag path.• Examination of errors on the first layer of the ribbon, where
tiles attach directly to tile adapters, revealed that nucleation is not perfect
• The copying error rate increased to 8% per tile and width-changing errors increased to 6% per layer.
Observations from the experiment:
• ‘‘0-Block’’ tiles appear gray• ‘‘1-Block’’ tiles appear white
Observations from the experiment:
• Fig F shows that the 1 block tiles are stopped
Observations from the experiment:
• The yellow part shows the error
Principles behind the success:
• Each tile set was capable of generating an infinite variety of distinct structures, a precondition for programmability
• A designed nucleation barrier prevented the spontaneous assembly of tiles in slightly supersaturated conditions, clearing the way for high-yield seeded growth with low error rates.
• Information contained in the seed was propagated, and sometimes processed, during crystal growth, enacting a simple developmental program.
The problems observed
• The rate of copying errors that changed 1 to 0 was 5–10 times higher than errors changing 0 to 1
• Premature reversal errors and spurious nucleation errors could be reduced by adding an independent nucleation barrier on the other edge of the ribbon
• Aggregation of crystals must be reduced.
• Implementing improved proofreading techniques (39) should further reduce logical error rates and larger block sizes may reduce internal lattice defects.
The Future Research topics
• The More efficient algorithm whose error rate is minimum that can compute the algorithmic complex structure of the seeded growth.
• More efficient tiles like origami rectangles which can be used to develop the structure or of even more complex organism.
• Getting the knowledge of combining the nanoparticles with the DNA can further help in developing new products in the field of nanotechnology
Top Related