Adaptive changes of maternal bone and intestine during lactation
Asst. Prof. Kannikar Wongdee, Ph.D.
Office of Academic Management, Faculty of Allied Health Sciences, Burapha UniversityCenter of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol UniversityE-mail: [email protected]
OHEC congress at Ambassador City Jomtien, ChonburiSeptember 14, 2012
Bone
Protect internal organs
Site for muscle attachment
Hematopoiesis
Locomotion
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99% of calcium is stored in bone.
Bone mass throughout life
Not only post-menopausal period, pregnancy and lactation also induce massive bone loss.
Bowman and Miller, J Musculoskel Neuron Interact, 2001; Charoenphandhu et al. Trends Endocrinol Metab, 2010
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Maternal calcium metabolism
Maternal calcium loss Fetal transfer during pregnancy (third trimester) Milk production (requires more calcium)
Calcium supply during lactation Bone — bone resorption Diet — intestinal calcium absorption
Charoenphandhu et al., Trends Endocrinol Metab, 2010Charoenphandhu et al. Trends Endocinol Metab, 2010
Adaptive changes of maternal
intestine and bone
Technical approach Histomorphometry: bone histomorphometry, micro-CT Cell biology and molecular biology techniques:
PCR, immunohistochemistryPicture:www.albany.edu; www.boripat.ac.th
Maternal villous hypertrophy during lactation
Wongdee K, Teerapornpuntakit J, Siangpro C, Chaipai S, Charoenphandhu N. Duodenal villous hypertrophy and upregulation of claudin-15 protein expression in lactating rats. Journal of Molecular Histology 2012 (in press).
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Wongdee et al., J Mol Histol, 2012
Maternal bone loss during lactation
Suntornsaratoon et al., Am J Physiol Endocrinol Metab, 2010
Human — up to 10% of skeletal mass Lactation-induced osteoporosis (Ofluoglu and Ofluoglu, 2008)
Rodent (6–8 pups) — up to 30% of skeletal mass
Partly under the regulation of PRL
Osteoclast surface Eroded surface
P = pregnancy; L = lactation; PW = postweaning
Picture: www.health.com
Roles of PRL on maternal calcium metabolism
Charoenphandhu N, Wongdee K, Krishnamra N. Is prolactin the cardinal calciotropic maternal hormone? Trends in Endocrinology & Metabolism 2010;21(7):395‒401.
Endochondral bone changes during lactation
Lactation-induced elongation of the long bone.
PRL-induced decreases in total growth plate height.
Suntornsaratoon et al. Histochem Cell Biol, 2010Wongdee et al. J Physiol Sci, 2012
Bone length Growth plate height
Perspective plan
• Fibroblast growth factor 23 (FGF23), bone-derived phosphate-regulating hormone
• Existence of FGF receptors in the intestinal villi.
• FGF23 compromised the 1,25(OH)2D3-induced intestinal calcium absorption.
Khuituan et al. Am J Physiol Endocrinol Metab, 2012
Role of FGF23 in bone and intestine during pregnancy and lactation
Acknowledgements
Assoc. Prof. Narattaphol Charoenphandhu, M.D., Ph.D. MUProf. Nateetip Krishnamra, Ph.D. MUChitchamai Siangpro, B.Sc. BUUSunitra Chaipai, B.Sc. BUUPanan Suntornsaratoon, M.Sc. MUNatchayaporn Thonapan, B.Sc MUJarinthorn Teerapornpuntakit, M.Sc. MU
Research grantsOffice of the Higher Education CommissionFaculty of Allied Health Sciences, Burapha UniversityThailand Research Fund (TRF)NSTDA
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