A Perfusion-based Micro 3-D Cell Culture Platform NTHU MSCL
Adviser: C. H. Liu C. He Yeh
Slide 2
A Perfusion-based Micro 3-D Cell Culture Platform Outline
Culture environment at large scale Culture environment at micro
scale Principle Fabrication Conclusion & Features Further
work
Slide 3
General Culture Equipment (1) (2) (3) (4) (5) (6) (7) (8) (9)
(10) (11) (12) (13) (14) (15) Lab On a Chip
Slide 4
On Chip Advantages Small dimension Saving sample Rapid assay
process (micro dimension, avoid medium or reagent diffusion time
gradient or concentration different ) Cheap, disposable
Slide 5
On Chip Culture Requirement Temperature : 37 pH : 7.2 ~ 7.4 (
95% air 5%CO 2 or HEPES) Buffered saline (PBS, PBSA, etc) Medium :
(Serum, glucose, amino acids, vitamin, antibiotics ) 3D culture (In
vivo Culture)
Slide 6
A Perfusion-based Micro 3-D Cell Culture Platform
Slide 7
Working Principle :
Slide 8
A Perfusion-based Micro 3-D Cell Culture Platform Fabrication
:
Slide 9
Conclusion Feature 1: 3D well-defined culture environment mimic
an in vivo environment distinct cellular behaviors (cell-cell and
cell-extracellular interactions ) provide more meaningful data
Slide 10
Conclusion Feature 2 : Short Loading cell channel Feature 3 :
High throughput (8 chambers)
Slide 11
Further work Bioassay (inject reagent instead of culture medium
in vivo bioassay in vivo bioassay) Biochemical experiment Tissue
engineering Drug screening TAS
Slide 12
Reference A PERFUSION-BASEDMICRO 3-D CELL CULTURE PLATFORM S.
B. Huang1, M. H. Wu2, Z. F. Cui3, Z. Cui2, J. L. Lin4 and G. B.
Lee1* MICROFLUIDIC DEVICES FOR THE ASSEMBLY AND CULTURE OF
THREE-DIMENSIONAL MULTI- CELLULAR CONSTRUCTS WITH DIFFUSION-LIMITED
MICROENVIRONMENTS M. Pinelis1, R. W. Kasinskas2, R. T. Borno1, J.
H. Park1, E. Chu3, N. S. Forbes2 and M. M. Maharbiz1
"Differentiation of human Embryonic Stem Cells into Hepatocytes in
2D and 3D culture system in vitro" The International Journal of
Developmental Biology, Vol. 50, pp. 645-652, 2006 "The multilayered
postconfluent cell culture as a model for drug screening" Critical
Reviews in Oncology/Hematol, Vol. 36, pp. 147-157, 2000. J. M.
Pardon; C.L. van der Wilt; K. Smid; E. Smitskamp-Wilms; H.H.
Baukus; P. E. Piazo; G. "Development of PDMS Microbioreactor with
Well-defined and Homogenous Culture Environment for Chondrocyte 3-D
Culture M. H. Wu, J. P. G. Urban, Z. Cui and Z. F. Cui, Biomedical
Microdevices, Vol. 8, pp. 331-340, 2006.