Zoology 2005 Part 2 Richard Mott. Inbred Mouse Strain Haplotype Structure When the genomes of a pair...

Zoology 2005 Part 2Richard Mott

Inbred Mouse Strain Haplotype StructureWhen the genomes of a pair of inbred strains are compared, we find a mosaic of segments of identity and difference (Wade et al, Nature 2002).A QTL segregating between the strains must lie in a region of sequence difference. What happens when we compare more than two strains simultaneously?

No Simple Haplotype Block MosaicYalcin et al 2004 PNAS

But a Tree Mosaic

In-silico MappingSimple idea-Collect phenotypes across a set of inbred strainsGenotype the strains (ONCE)Look for phenotype-genotype correlationWorks well for simple Mendelian traits (eg coat colour)Suggested as a panacea for QTL mapping

In-silico Mapping ProblemsLess well-suited for complex traitsNumber of strains required grows quickly with the complexity of the trait. Suggested at least 100 strains required, possibly more if epistasis is presentRequire high-density genotype/sequence data to ensure identity-by-state = identity by-descentMay be very useful for the dissection of a QTL previously identified in a F2 cross (look for patterns of sequence difference)

Recombinant Inbred LinesPanels of inbred lines descended form pairs of inbred strainsGenomes are inbred mosaics of the foundersLines only need be genotyped onceSimilar to in-silico mapping exceptidentity-by-descent=identity-by-stateCoarser recombination structure?lower resolution mapping?

BXD chromosome 4

Testing if a variant is functional without genotyping it(Yalcin et al, Genetics 2005)Requirements:A Heterogeneous Stock, genotyped at a skeleton of markersThe genome sequences of the progenitor strainsA statistical test

Merge AnalysisEach polymorphism groups together the founders according to their allelesIf the polymorphism is functional, then a model in which the phenotypic strain effects are estimated after merging the strains together should be as good as a model where each strain can have an independent effect.Compare the fit of merged and unmerged genetic models to test if the variant is functional.If the fit of the merged model is poor then that variant can be eliminated.

Merge Analysis

How can we show a gene under a QTL peak affects the trait?Genetic Mapping identifies Functional Variants, not GenesCould be a control element affecting some other gene

Quantitative ComplementationKO0

Quantitative ComplementationKOHighLow05010030wt

Quantitative ComplementationKOHighLow05010030dwt

Quantitative ComplementationKOHighLow05010030ddD = d - dwt

Using Functional Information to Confirm GenesFurther experimentsfurther bioinformatics, eg networks, functional annotation (GO, KEGG)candidate gene sequencinggene expression analyses (eQTL) offounder strainsHS

Mouse/human sequence comparison

Enhancer reporter assaysluciferase reporterpromoterenhancerluciferase reporterpromoterenhancer

Enhancer elements affect promoter expression

Chart2

1.10064759451.60374162120.932801532

1.97974739842.46831179072.6776536969

241119624111962411196

0.66979836421.90891912141.0790392477

14.157866318321.954514979425.4962303755

4.277300403615.14452608782.7325210865

24188500.35166348150.39307309

7.183674400234.311684309835.3357305594

1.4421434669121.7123275814121.9832159119

0.6368279790.41316948330.4546720059

2.23769235643.04722832322.1080126298

1.79591471991.49237438341.5940182713

1.4417126634.2900850863.3141571834

26853951.64941845172.1292517223

1.43464705210.66920994321.6889186103

1.81339384521.6790660141.8884733816

0.42653739461.20013626180.8586912708

32268372.21789686771.5520868972

0.97521683292.11132596841.8259738271

41078601.00461561810.4074829637

NIH3T3

ND7

Neuro2a

Position

Fold expression difference (C57/AJ)

PICO_GREEN_DATA

did 1/20 dilution in TE, then took 3ul and added 150ul TE for pico green assay

pico to ng/ul conversion factor = 2920.91actual concentration ng/ul

ul addedug addedpico valueconversion to ng/ulratio of c57/AJ concentrationsx dilution factor (1000)

A1pGL3_C113010.1030821918103.0821917808

A2PGL3_B1118860.645890411645.8904109589

A33B11121960.7518835616751.8835616438

A46G101117720.60667808220.8068777044606.6780821918

A550A11111050.3784246575378.4246575342

A650E121124170.8277397262.1873303167827.7397260274

A746A121114530.4976027397497.602739726

A846A111120790.71198630141.4308327598711.9863013699

A945F16.915830.199486301428.9110581695

A1047E26.8516050.20702054791.037768240330.2219778022

A1145F111.17122600.7738013699661.3686921906

A1247H91.18114880.50958904110.6585527772431.8551195728

B145B7116680.2287671233228.7671232877

B247E7116380.21832191780.9543413174218.3219178082

B346G31110830.370890411370.8904109589

B448G91117210.58921232881.5886426593589.2123287671

B546B61115280.5232876712523.2876712329

B648H111111170.38253424660.7310209424382.5342465753

B746A9113750.1284246575128.4246575342

B849A11114680.5027397263.9146666667502.7397260274

B932E31114170.4851027397485.102739726

B1034D51119960.6833904111.4087539711683.3904109589

B1131C51119170.6565068493656.5068493151

B1234C61115150.51883561640.7902973396518.8356164384

C132C103.3315570.190582191857.2318894237

C235G21126100.89383561644.6900269542893.8356164384

C333F41114820.5075342466507.5342465753

C436B31114440.49434931510.9740215924494.3493150685

C548H71114520.4970890411497.0890410959

C649B51117830.61061643841.2283844299610.6164383562

C746B21111290.3866438356386.6438356164

C851B81111350.38869863011.0053144376388.698630137

C932E81112340.4226027397422.602739726

C1034B81114450.49469178081.1705834684494.6917808219

C1132D91111170.3825342466382.5342465753

C1234H101113330.45633561641.1929274843456.3356164384

D13C101117590.6023972603602.397260274

D224C111113580.46506849320.7720295623465.0684931507

D311G71113390.4585616438458.5616438356

D452G94.6219710.33253424660.725168035871.9771096483

D529F23.35112380.4238013699126.5078716009

D6AJ_21.64119390.66404109591.5668686869404.9031072503

D7C57_23.27115270.5227739726159.8697163923

D856G91113860.47448630140.9076318375474.4863013699

D948C1119800.3354452055335.4452054795

D1065A81114230.48732876711.4527820316487.3287671233

D1165C111110280.3518835616351.8835616438

D1265G41115360.52602739731.4948905109526.0273972603

E133F31.9419620.3294520548169.8206468013

E265A21113160.45051369861.3674636175450.5136986301

E373G11.07117180.5881849315549.7055434643

E448E8119460.32397260270.5508005822323.9726027397

E565C81112340.4226027397422.602739726

E666D51114420.49383561641.1685575365493.8356164384

E767H51116200.5546232877554.6232876712

E867D91.05114210.48664383560.877431306463.4703196347

E958G31112470.4268835616426.8835616438

E1070E61111470.39280821920.9201764942392.8082191781

E1168B11.1117050.5839041096530.8219178082

E1266A91112740.43630136990.7472140762436.301369863

F167A21.06113170.4510273973425.4975445852

F246A93.6713660.12517123290.277524677334.1066029637

background80.0027397262.7397260274

background100.00325342473.2534246575

background50.00171232881.7123287671

background70.00222602742.2260273973

background80.0027397262.7397260274

background80.0027397262.7397260274

background70.00239726032.397260274

background80.0027397262.7397260274

background70.00222602742.2260273973

background60.00205479452.0547945205

PLATE_SETUP

clone namevectorenhancerstrainsample IDcell lysate plateplate positioncell line

cotrasfec 1cotrasfec 2

1pCMV_1pGL3_control1, 2, 3TRANSFECTION_PLATE_1A 1 - 3NIH3T3 - PX

2pCMV_1pGL3_basic4, 5, 6TRANSFECTION_PLATE_1A 4 - 6NIH3T3 - PX

3pCMV_13B1RGS18_SNP5196_1F_SmaIAJ7, 8, 9TRANSFECTION_PLATE_1A 7 - 9NIH3T3 - PX

4pCMV_16G10RGS18_SNP5196_1F_SmaIC5710, 11, 12TRANSFECTION_PLATE_1A 10 - 12NIH3T3 - PX

5pCMV_150A1RGS18_SNP5196_1F_SmaIENH_3110794AJ13, 14, 15TRANSFECTION_PLATE_1B 13 - 15NIH3T3 - PX

6pCMV_150E12RGS18_SNP5196_1F_SmaIENH_3110794C5716, 17, 18TRANSFECTION_PLATE_1B 16 - 18NIH3T3 - PX

7pCMV_146A12RGS18_SNP5196_1F_SmaIENH_2532527AJ19, 20, 21TRANSFECTION_PLATE_1B 19 - 21NIH3T3 - PX

8pCMV_146A11RGS18_SNP5196_1F_SmaIENH_2532527C5722, 23, 24TRANSFECTION_PLATE_1B 22 - 24NIH3T3 - PX

9pCMV_145F1RGS18_SNP5196_1F_SmaIENH_2156454AJ25, 26, 27TRANSFECTION_PLATE_1C 25 - 27NIH3T3 - PX

10pCMV_147E2RGS18_SNP5196_1F_SmaIENH_2156454C5728, 29, 30TRANSFECTION_PLATE_1C 28 - 30NIH3T3 - PX

11pCMV_145F11RGS18_SNP5196_1F_SmaIENH_2543708AJ31, 32, 33TRANSFECTION_PLATE_1C 31 - 33NIH3T3 - PX

12pCMV_147H9RGS18_SNP5196_1F_SmaIENH_2543708C5734, 35, 36TRANSFECTION_PLATE_1C 34 - 36NIH3T3 - PX

13pCMV_145B7RGS18_SNP5196_1F_SmaIENH_2558562AJTRANSFECTION_PLATE_1D 37 - 39NIH3T3 - PXmixed up sample order, ie 37, 38, 40, 39, 41, 42

14pCMV_147E7RGS18_SNP5196_1F_SmaIENH_2558562C57TRANSFECTION_PLATE_1D 40 - 42NIH3T3 - PXadded all but 180ul of sample 43 to tube 42

15pCMV_146G3RGS18_SNP5196_1F_SmaIENH_2682967AJTRANSFECTION_PLATE_1D 43 - 45NIH3T3 - PXused 5ul of remaining 43 for luciferase assay, 10ul Bgal assay

16pCMV_148G9RGS18_SNP5196_1F_SmaIENH_2682967C5746, 47, 48TRANSFECTION_PLATE_1D 46 - 48NIH3T3 - PX

17pCMV_146B6RGS18_SNP5196_1F_SmaIENH_2490240AJ49, 50, 51TRANSFECTION_PLATE_1E 49 - 51NIH3T3 - PX

18pCMV_148H11RGS18_SNP5196_1F_SmaIENH_2490240C5752, 53, 54TRANSFECTION_PLATE_1E 52 - 54NIH3T3 - PX

19pCMV_146A9RGS18_SNP5196_1F_SmaIENH_2499645AJ55, 56, 57TRANSFECTION_PLATE_1E 55 - 57NIH3T3 - PX

20pCMV_149A1RGS18_SNP5196_1F_SmaIENH_2499645C5758, 59, 60TRANSFECTION_PLATE_1E 58 - 60NIH3T3 - PX

21pCMV_132E3RGS18_SNP5196_1F_SmaIENH_2404529AJ61, 62, 63TRANSFECTION_PLATE_1F 61 - 63NIH3T3 - PX

22pCMV_134D5RGS18_SNP5196_1F_SmaIENH_2404529C5764, 65, 66TRANSFECTION_PLATE_1F 64 - 66NIH3T3 - PX

23pCMV_131C5RGS18_SNP5196_1F_SmaIENH_2409307AJ67, 68, 69TRANSFECTION_PLATE_1F 67 - 69NIH3T3 - PX

24pCMV_134C6RGS18_SNP5196_1F_SmaIENH_2409307C5770, 71, 72TRANSFECTION_PLATE_1F 70 - 72NIH3T3 - PX

25pCMV_132C10RGS18_SNP5196_1F_SmaIENH_2417081AJ73, 74, 75TRANSFECTION_PLATE_1G 73 - 75NIH3T3 - PX

26pCMV_135G2RGS18_SNP5196_1F_SmaIENH_2417081C5776, 77, 78TRANSFECTION_PLATE_1G 76 - 78NIH3T3 - PX

27pCMV_133F4RGS18_SNP5196_1F_SmaIENH_2833990AJ79, 80, 81TRANSFECTION_PLATE_1G 79 - 81NIH3T3 - PX

28pCMV_136B3RGS18_SNP5196_1F_SmaIENH_2833990C5782, 83, 84TRANSFECTION_PLATE_1G 82 - 84NIH3T3 - PX

29pCMV_148H7RGS18_SNP5196_1F_SmaIENH_4053050AJ85, 86, 87TRANSFECTION_PLATE_1H 85 - 87NIH3T3 - PX

30pCMV_149B5RGS18_SNP5196_1F_SmaIENH_4053050C5788, 89, 90TRANSFECTION_PLATE_1H 88 - 90NIH3T3 - PX

31blank91, 92, 93TRANSFECTION_PLATE_1H 91 - 93NIH3T3 - PX

32blank94, 95, 96TRANSFECTION_PLATE_1H 94 - 96NIH3T3 - PX

1pCMV_1pGL3_control1, 2, 3TRANSFECTION_PLATE_2A 1 - 3NIH3T3 - PXII

2pCMV_1pGL3_basic4, 5, 6TRANSFECTION_PLATE_2A 4 - 6NIH3T3 - PXII

3pCMV_13B1RGS18_SNP5196_1F_SmaIAJ7, 8, 9TRANSFECTION_PLATE_2A 7 - 9NIH3T3 - PXII

4pCMV_16G10RGS18_SNP5196_1F_SmaIC5710, 11, 12TRANSFECTION_PLATE_2A 10 - 12NIH3T3 - PXII

5pCMV_150A1RGS18_SNP5196_1F_SmaIENH_3110794AJ13, 14, 15TRANSFECTION_PLATE_2B 13 - 15NIH3T3 - PXII

6pCMV_150E12RGS18_SNP5196_1F_SmaIENH_3110794C5716, 17, 18TRANSFECTION_PLATE_2B 16 - 18NIH3T3 - PXII

7pCMV_146A12RGS18_SNP5196_1F_SmaIENH_2532527AJ19, 20, 21TRANSFECTION_PLATE_2B 19 - 21NIH3T3 - PXII

8pCMV_146A11RGS18_SNP5196_1F_SmaIENH_2532527C5722, 23, 24TRANSFECTION_PLATE_2B 22 - 24NIH3T3 - PXII

9pCMV_145F1RGS18_SNP5196_1F_SmaIENH_2156454AJ25, 26, 27TRANSFECTION_PLATE_2C 25 - 27NIH3T3 - PXII

10pCMV_147E2RGS18_SNP5196_1F_SmaIENH_2156454C5728, 29, 30TRANSFECTION_PLATE_2C 28 - 30NIH3T3 - PXII

11pCMV_145F11RGS18_SNP5196_1F_SmaIENH_2543708AJ31, 32, 33TRANSFECTION_PLATE_2C 31 - 33NIH3T3 - PXII

12pCMV_147H9RGS18_SNP5196_1F_SmaIENH_2543708C5734, 35, 36TRANSFECTION_PLATE_2C 34 - 36NIH3T3 - PXII

13pCMV_145B7RGS18_SNP5196_1F_SmaIENH_2558562AJ37, 38, 39TRANSFECTION_PLATE_2D 37 - 39NIH3T3 - PXII

14pCMV_147E7RGS18_SNP5196_1F_SmaIENH_2558562C5740, 41, 42TRANSFECTION_PLATE_2D 40 - 42NIH3T3 - PXII

15pCMV_146G3RGS18_SNP5196_1F_SmaIENH_2682967AJ43, 44, 45TRANSFECTION_PLATE_2D 43 - 45NIH3T3 - PXII

16pCMV_148G9RGS18_SNP5196_1F_SmaIENH_2682967C5746, 47, 48TRANSFECTION_PLATE_2D 46 - 48NIH3T3 - PXII

17pCMV_146B6RGS18_SNP5196_1F_SmaIENH_2490240AJ49, 50, 51TRANSFECTION_PLATE_2E 49 - 51NIH3T3 - PXII

18pCMV_148H11RGS18_SNP5196_1F_SmaIENH_2490240C5752, 53, 54TRANSFECTION_PLATE_2E 52 - 54NIH3T3 - PXII

19pCMV_146A9RGS18_SNP5196_1F_SmaIENH_2499645AJ55, 56, 57TRANSFECTION_PLATE_2E 55 - 57NIH3T3 - PXII

20pCMV_149A1RGS18_SNP5196_1F_SmaIENH_2499645C5758, 59, 60TRANSFECTION_PLATE_2E 58 - 60NIH3T3 - PXII

21pCMV_132E3RGS18_SNP5196_1F_SmaIENH_2404529AJ61, 62, 63TRANSFECTION_PLATE_2F 61 - 63NIH3T3 - PXII

22pCMV_134D5RGS18_SNP5196_1F_SmaIENH_2404529C5764, 65, 66TRANSFECTION_PLATE_2F 64 - 66NIH3T3 - PXII

23pCMV_131C5RGS18_SNP5196_1F_SmaIENH_2409307AJ67, 68, 69TRANSFECTION_PLATE_2F 67 - 69NIH3T3 - PXII

24pCMV_134C6RGS18_SNP5196_1F_SmaIENH_2409307C5770, 71, 72TRANSFECTION_PLATE_2F 70 - 72NIH3T3 - PXII

25pCMV_132C10RGS18_SNP5196_1F_SmaIENH_2417081AJ73, 74, 75TRANSFECTION_PLATE_2G 73 - 75NIH3T3 - PXII

26pCMV_135G2RGS18_SNP5196_1F_SmaIENH_2417081C5776, 77, 78TRANSFECTION_PLATE_2G 76 - 78NIH3T3 - PXII

27pCMV_133F4RGS18_SNP5196_1F_SmaIENH_2833990AJ79, 80, 81TRANSFECTION_PLATE_2G 79 - 81NIH3T3 - PXII

28pCMV_136B3RGS18_SNP5196_1F_SmaIENH_2833990C5782, 83, 84TRANSFECTION_PLATE_2G 82 - 84NIH3T3 - PXII

29pCMV_148H7RGS18_SNP5196_1F_SmaIENH_4053050AJ85, 86, 87TRANSFECTION_PLATE_2H 85 - 87NIH3T3 - PXII

30pCMV_149B5RGS18_SNP5196_1F_SmaIENH_4053050C5788, 89, 90TRANSFECTION_PLATE_2H 88 - 90NIH3T3 - PXII

31pCMV_146B2RGS18_SNP5196_1F_SmaIENH_2930303AJ91, 92, 93TRANSFECTION_PLATE_2H 91 - 93NIH3T3 - PXII

32pCMV_151B8RGS18_SNP5196_1F_SmaIENH_2930303C5794, 95, 96TRANSFECTION_PLATE_2H 94 - 96NIH3T3 - PXII

33pCMV_132E8RGS18_SNP5196_1F_SmaIENH_2413450AJ97, 98, 99TRANSFECTION_PLATE_3A 1 - 3NIH3T3 - PXII

34pCMV_134B8RGS18_SNP5196_1F_SmaIENH_2413450C57100, 101, 102TRANSFECTION_PLATE_3A 4 - 6NIH3T3 - PXII

35pCMV_132D9RGS18_SNP5196_1F_SmaIENH_2415436AJ103, 104, 105TRANSFECTION_PLATE_3A 7 - 9NIH3T3 - PXII

36pCMV_134H10RGS18_SNP5196_1F_SmaIENH_2415436C57106, 107, 108TRANSFECTION_PLATE_3A 10 - 12NIH3T3 - PXII

37pCMV_13C10BMP_prom_ex1_1AJ109, 110, 111TRANSFECTION_PLATE_3B 13 - 15NIH3T3 - PXII

38pCMV_124C11BMP_prom_ex1_1C57112, 113, 114TRANSFECTION_PLATE_3B 16 - 18NIH3T3 - PXII

39pCMV_111G7RGS1SNP2885_2FAJ115, 116, 117TRANSFECTION_PLATE_3B 19 - 21NIH3T3 - PXII

40pCMV_129F2RGS1SNP2885_2FRIII118, 119, 120TRANSFECTION_PLATE_3B 22 - 24NIH3T3 - PXII

41pCMV_152G9RGS1SNP2885_2FC57121, 122, 123TRANSFECTION_PLATE_3C 25 - 27NIH3T3 - PXII

42pCMV_1AJ_2RGS18_SNP5196 (binnaz)AJ124, 125, 126TRANSFECTION_PLATE_3C 28 - 30NIH3T3 - PXII

43pCMV_1C57_2RGS18_SNP5196 (binnaz)C57127, 128, 129TRANSFECTION_PLATE_3C 31 - 33NIH3T3 - PXII

44pCMV_158G3RGS2_Func8F_SmaI/Func5RAJ130, 131, 132TRANSFECTION_PLATE_3C 34 - 36NIH3T3 - PXII

45pCMV_156G9RGS1_5prime2FC57133, 134, 135TRANSFECTION_PLATE_3D 37 - 39NIH3T3 - PXII

46pCMV_148C1RGS18_SNP5196_1F_SmaIENH_2411196AJ136, 137, 138TRANSFECTION_PLATE_3D 40 - 42NIH3T3 - PXII

47pCMV_148E8RGS18_SNP5196_1F_SmaIENH_4107860AJ139, 140, 141TRANSFECTION_PLATE_3D 43 - 45NIH3T3 - PXII

48untransfectedfugene6 + DMEM142, 143, 144TRANSFECTION_PLATE_3D 46 - 48NIH3T3 - PXII

NOTHING145, 146, 147TRANSFECTION_PLATE_3E 49 - 51NIH3T3 - PXII




NOTHING157, 158, 159TRANSFECTION_PLATE_3F 61 - 63NIH3T3 - PXII




NOTHING169, 170, 171TRANSFECTION_PLATE_3G 73 - 75NIH3T3 - PXII




NOTHING181, 182, 183TRANSFECTION_PLATE_3H 85 - 87NIH3T3 - PXII




1pCMV_1pGL3_control1, 2, 3TRANSFECTION_PLATE_4A 1 - 3Neuro2a - PV

2pCMV_1pGL3_basic4, 5, 6TRANSFECTION_PLATE_4A 4 - 6Neuro2a - PV

3pCMV_13B1RGS18_SNP5196_1F_SmaIAJ7, 8, 9TRANSFECTION_PLATE_4A 7 - 9Neuro2a - PV

4pCMV_16G10RGS18_SNP5196_1F_SmaIC5710, 11, 12TRANSFECTION_PLATE_4A 10 - 12Neuro2a - PV

5pCMV_150A1RGS18_SNP5196_1F_SmaIENH_3110794AJ13, 14, 15TRANSFECTION_PLATE_4B 13 - 15Neuro2a - PV

6pCMV_150E12RGS18_SNP5196_1F_SmaIENH_3110794C5716, 17, 18TRANSFECTION_PLATE_4B 16 - 18Neuro2a - PV

7pCMV_146A12RGS18_SNP5196_1F_SmaIENH_2532527AJ19, 20, 21TRANSFECTION_PLATE_4B 19 - 21Neuro2a - PV

8pCMV_146A11RGS18_SNP5196_1F_SmaIENH_2532527C5722, 23, 24TRANSFECTION_PLATE_4B 22 - 24Neuro2a - PV

9pCMV_145F1RGS18_SNP5196_1F_SmaIENH_2156454AJ25, 26, 27TRANSFECTION_PLATE_4C 25 - 27Neuro2a - PV

10pCMV_147E2RGS18_SNP5196_1F_SmaIENH_2156454C5728, 29, 30TRANSFECTION_PLATE_4C 28 - 30Neuro2a - PV

11pCMV_145F11RGS18_SNP5196_1F_SmaIENH_2543708AJ31, 32, 33TRANSFECTION_PLATE_4C 31 - 33Neuro2a - PV

12pCMV_147H9RGS18_SNP5196_1F_SmaIENH_2543708C5734, 35, 36TRANSFECTION_PLATE_4C 34 - 36Neuro2a - PV

13pCMV_145B7RGS18_SNP5196_1F_SmaIENH_2558562AJ37, 38, 39TRANSFECTION_PLATE_4D 37 - 39Neuro2a - PV

14pCMV_147E7RGS18_SNP5196_1F_SmaIENH_2558562C57TRANSFECTION_PLATE_4D 40 - 42Neuro2a - PVused same tip as 45B7 (39)

15pCMV_146G3RGS18_SNP5196_1F_SmaIENH_2682967AJ43, 44, 45TRANSFECTION_PLATE_4D 43 - 45Neuro2a - PV

16pCMV_148G9RGS18_SNP5196_1F_SmaIENH_2682967C5746, 47, 48TRANSFECTION_PLATE_4D 46 - 48Neuro2a - PV

17pCMV_146B6RGS18_SNP5196_1F_SmaIENH_2490240AJ49, 50, 51TRANSFECTION_PLATE_4E 49 - 51Neuro2a - PV

18pCMV_148H11RGS18_SNP5196_1F_SmaIENH_2490240C57TRANSFECTION_PLATE_4E 52 - 54Neuro2a - PVcells contaminated - media orange

19pCMV_146A9RGS18_SNP5196_1F_SmaIENH_2499645AJ55, 56, 57TRANSFECTION_PLATE_4E 55 - 57Neuro2a - PV

20pCMV_149A1RGS18_SNP5196_1F_SmaIENH_2499645C5758, 59, 60TRANSFECTION_PLATE_4E 58 - 60Neuro2a - PV

21pCMV_132E3RGS18_SNP5196_1F_SmaIENH_2404529AJ61, 62, 63TRANSFECTION_PLATE_4F 61 - 63Neuro2a - PV

22pCMV_134D5RGS18_SNP5196_1F_SmaIENH_2404529C5764, 65, 66TRANSFECTION_PLATE_4F 64 - 66Neuro2a - PV

23pCMV_131C5RGS18_SNP5196_1F_SmaIENH_2409307AJ67, 68, 69TRANSFECTION_PLATE_4F 67 - 69Neuro2a - PV

24pCMV_134C6RGS18_SNP5196_1F_SmaIENH_2409307C5770, 71, 72TRANSFECTION_PLATE_4F 70 - 72Neuro2a - PV

25pCMV_132C10RGS18_SNP5196_1F_SmaIENH_2417081AJ73, 74, 75TRANSFECTION_PLATE_4G 73 - 75Neuro2a - PV

26pCMV_135G2RGS18_SNP5196_1F_SmaIENH_2417081C5776, 77, 78TRANSFECTION_PLATE_4G 76 - 78Neuro2a - PV

27pCMV_133F4RGS18_SNP5196_1F_SmaIENH_2833990AJ79, 80, 81TRANSFECTION_PLATE_4G 79 - 81Neuro2a - PV

28pCMV_136B3RGS18_SNP5196_1F_SmaIENH_2833990C5782, 83, 84TRANSFECTION_PLATE_4G 82 - 84Neuro2a - PV

29pCMV_148H7RGS18_SNP5196_1F_SmaIENH_4053050AJ85, 86, 87TRANSFECTION_PLATE_4H 85 - 87Neuro2a - PV

30pCMV_149B5RGS18_SNP5196_1F_SmaIENH_4053050C5788, 89, 90TRANSFECTION_PLATE_4H 88 - 90Neuro2a - PV

31pCMV_146B2RGS18_SNP5196_1F_SmaIENH_2930303AJ91, 92, 93TRANSFECTION_PLATE_4H 91 - 93Neuro2a - PV

32pCMV_151B8RGS18_SNP5196_1F_SmaIENH_2930303C5794, 95, 96TRANSFECTION_PLATE_4H 94 - 96Neuro2a - PV

33pCMV_132E8RGS18_SNP5196_1F_SmaIENH_2413450AJ97, 98, 99TRANSFECTION_PLATE_5A 1 - 3Neuro2a - PV

34pCMV_134B8RGS18_SNP5196_1F_SmaIENH_2413450C57100, 101, 102TRANSFECTION_PLATE_5A 4 - 6Neuro2a - PV

35pCMV_132D9RGS18_SNP5196_1F_SmaIENH_2415436AJ103, 104, 105TRANSFECTION_PLATE_5A 7 - 9Neuro2a - PV

36pCMV_134H10RGS18_SNP5196_1F_SmaIENH_2415436C57106, 107, 108TRANSFECTION_PLATE_5A 10 - 12Neuro2a - PV

37pCMV_13C10BMP_prom_ex1_1AJ109, 110, 111TRANSFECTION_PLATE_5B 13 - 15Neuro2a - PV

38pCMV_124C11BMP_prom_ex1_1C57112, 113, 114TRANSFECTION_PLATE_5B 16 - 18Neuro2a - PV

39pCMV_111G7RGS1SNP2885_2FAJ115, 116, 117TRANSFECTION_PLATE_5B 19 - 21Neuro2a - PV

40pCMV_129F2RGS1SNP2885_2FRIII118, 119, 120TRANSFECTION_PLATE_5B 22 - 24Neuro2a - PV

41pCMV_152G9RGS1SNP2885_2FC57121, 122, 123TRANSFECTION_PLATE_5C 25 - 27Neuro2a - PV

42pCMV_1AJ_2RGS18_SNP5196 (binnaz)AJ124, 125, 126TRANSFECTION_PLATE_5C 28 - 30Neuro2a - PV

43pCMV_1C57_2RGS18_SNP5196 (binnaz)C57127, 128, 129TRANSFECTION_PLATE_5C 31 - 33Neuro2a - PV

44pCMV_158G3RGS2_Func8F_SmaI/Func5RAJ130, 131, 132TRANSFECTION_PLATE_5C 34 - 36Neuro2a - PV

45pCMV_156G9RGS1_5prime2FC57133, 134, 135TRANSFECTION_PLATE_5D 37 - 39Neuro2a - PV

46pCMV_148C1RGS18_SNP5196_1F_SmaIENH_2411196AJ136, 137, 138TRANSFECTION_PLATE_5D 40 - 42Neuro2a - PV

47pCMV_148E8RGS18_SNP5196_1F_SmaIENH_4107860AJ139, 140, 141TRANSFECTION_PLATE_5D 43 - 45Neuro2a - PV

48cells only blank142, 143, 144TRANSFECTION_PLATE_5D 46 - 48Neuro2a - PV

49pCMV_13B1RGS18_SNP5196_1F_SmaIAJ145, 146, 147TRANSFECTION_PLATE_5E 49 - 51Neuro2a - PV

50pCMV_16G10RGS18_SNP5196_1F_SmaIC57148, 149, 150TRANSFECTION_PLATE_5E 52 - 54Neuro2a - PVnot transfected - cells only as dropped plate before transfection

51pCMV_150A1RGS18_SNP5196_1F_SmaIENH_3110794AJ151, 152, 153TRANSFECTION_PLATE_5E 55 - 57Neuro2a - PV

52pCMV_150E12RGS18_SNP5196_1F_SmaIENH_3110794C57154, 155, 156TRANSFECTION_PLATE_5E 58 - 60Neuro2a - PV

53pCMV_146A12RGS18_SNP5196_1F_SmaIENH_2532527AJ157, 158, 159TRANSFECTION_PLATE_5F 61 - 63Neuro2a - PV

54pCMV_146A11RGS18_SNP5196_1F_SmaIENH_2532527C57160, 161, 162TRANSFECTION_PLATE_5F 64 - 66Neuro2a - PV

55pCMV_145F1RGS18_SNP5196_1F_SmaIENH_2156454AJ163, 164, 165TRANSFECTION_PLATE_5F 67 - 69Neuro2a - PV

56pCMV_147E2RGS18_SNP5196_1F_SmaIENH_2156454C57166, 167, 168TRANSFECTION_PLATE_5F 70 - 72Neuro2a - PV

57pCMV_145F11RGS18_SNP5196_1F_SmaIENH_2543708AJ169, 170, 171TRANSFECTION_PLATE_5G 73 - 75Neuro2a - PV

58pCMV_147H9RGS18_SNP5196_1F_SmaIENH_2543708C57172, 173, 174TRANSFECTION_PLATE_5G 76 - 78Neuro2a - PV

59pCMV_145B7RGS18_SNP5196_1F_SmaIENH_2558562AJ175, 176, 177TRANSFECTION_PLATE_5G 79 - 81Neuro2a - PV

60pCMV_147E7RGS18_SNP5196_1F_SmaIENH_2558562C57178, 179, 180TRANSFECTION_PLATE_5G 82 - 84Neuro2a - PV

61pCMV_146G3RGS18_SNP5196_1F_SmaIENH_2682967AJ181, 182, 183TRANSFECTION_PLATE_5H 85 - 87Neuro2a - PVdropped plate after transfection so probable cross contamination

62pCMV_148G9RGS18_SNP5196_1F_SmaIENH_2682967C57184, 185, 186TRANSFECTION_PLATE_5H 88 - 90Neuro2a - PV

63pCMV_1MUTpCS2+MTS140GMUT187, 188, 189TRANSFECTION_PLATE_5H 91 - 93Neuro2a - PV

64pCMV_1WTpCS2+MTWTWT190, 191, 192TRANSFECTION_PLATE_5H 94 - 96Neuro2a - PV

1pCMV_1pGL3_control1, 2, 3TRANSFECTION_PLATE_6A 1 - 3Neuro2a - PVI

2pCMV_1pGL3_basic4, 5, 6TRANSFECTION_PLATE_6A 4 - 6Neuro2a - PVI

3pCMV_13B1RGS18_SNP5196_1F_SmaIAJ7, 8, 9TRANSFECTION_PLATE_6A 7 - 9Neuro2a - PVI

4pCMV_16G10RGS18_SNP5196_1F_SmaIC5710, 11, 12TRANSFECTION_PLATE_6A 10 - 12Neuro2a - PVI

5pCMV_150A1RGS18_SNP5196_1F_SmaIENH_3110794AJ13, 14, 15TRANSFECTION_PLATE_6B 13 - 15Neuro2a - PVI

6pCMV_150E12RGS18_SNP5196_1F_SmaIENH_3110794C5716, 17, 18TRANSFECTION_PLATE_6B 16 - 18Neuro2a - PVI

7pCMV_146A12RGS18_SNP5196_1F_SmaIENH_2532527AJ19, 20, 21TRANSFECTION_PLATE_6B 19 - 21Neuro2a - PVI

8pCMV_146A11RGS18_SNP5196_1F_SmaIENH_2532527C5722, 23, 24TRANSFECTION_PLATE_6B 22 - 24Neuro2a - PVI

9pCMV_145F1RGS18_SNP5196_1F_SmaIENH_2156454AJ25, 26, 27TRANSFECTION_PLATE_6C 25 - 27Neuro2a - PVI

10pCMV_147E2RGS18_SNP5196_1F_SmaIENH_2156454C5728, 29, 30TRANSFECTION_PLATE_6C 28 - 30Neuro2a - PVI

11pCMV_145F11RGS18_SNP5196_1F_SmaIENH_2543708AJ31, 32, 33TRANSFECTION_PLATE_6C 31 - 33Neuro2a - PVI

12pCMV_147H9RGS18_SNP5196_1F_SmaIENH_2543708C5734, 35, 36TRANSFECTION_PLATE_6C 34 - 36Neuro2a - PVI

13pCMV_145B7RGS18_SNP5196_1F_SmaIENH_2558562AJ37, 38, 39TRANSFECTION_PLATE_6D 37 - 39Neuro2a - PVI

14pCMV_147E7RGS18_SNP5196_1F_SmaIENH_2558562C5740, 41, 42TRANSFECTION_PLATE_6D 40 - 42Neuro2a - PVI

15pCMV_146G3RGS18_SNP5196_1F_SmaIENH_2682967AJ43, 44, 45TRANSFECTION_PLATE_6D 43 - 45Neuro2a - PVI

16pCMV_148G9RGS18_SNP5196_1F_SmaIENH_2682967C5746, 47, 48TRANSFECTION_PLATE_6D 46 - 48Neuro2a - PVI

17pCMV_146B6RGS18_SNP5196_1F_SmaIENH_2490240AJ49, 50, 51TRANSFECTION_PLATE_6E 49 - 51Neuro2a - PVI

18pCMV_148H11RGS18_SNP5196_1F_SmaIENH_2490240C5752, 53, 54TRANSFECTION_PLATE_6E 52 - 54Neuro2a - PVI

19pCMV_146A9RGS18_SNP5196_1F_SmaIENH_2499645AJ55, 56, 57TRANSFECTION_PLATE_6E 55 - 57Neuro2a - PVI

20pCMV_149A1RGS18_SNP5196_1F_SmaIENH_2499645C5758, 59, 60TRANSFECTION_PLATE_6E 58 - 60Neuro2a - PVI

21pCMV_132E3RGS18_SNP5196_1F_SmaIENH_2404529AJ61, 62, 63TRANSFECTION_PLATE_6F 61 - 63Neuro2a - PVI

22pCMV_134D5RGS18_SNP5196_1F_SmaIENH_2404529C5764, 65, 66TRANSFECTION_PLATE_6F 64 - 66Neuro2a - PVI

23pCMV_131C5RGS18_SNP5196_1F_SmaIENH_2409307AJ67, 68, 69TRANSFECTION_PLATE_6F 67 - 69Neuro2a - PVI

24pCMV_134C6RGS18_SNP5196_1F_SmaIENH_2409307C5770, 71, 72TRANSFECTION_PLATE_6F 70 - 72Neuro2a - PVI

25pCMV_132C10RGS18_SNP5196_1F_SmaIENH_2417081AJ73, 74, 75TRANSFECTION_PLATE_6G 73 - 75Neuro2a - PVI

26pCMV_135G2RGS18_SNP5196_1F_SmaIENH_2417081C5776, 77, 78TRANSFECTION_PLATE_6G 76 - 78Neuro2a - PVI

27pCMV_133F4RGS18_SNP5196_1F_SmaIENH_2833990AJ79, 80, 81TRANSFECTION_PLATE_6G 79 - 81Neuro2a - PVI

28pCMV_136B3RGS18_SNP5196_1F_SmaIENH_2833990C5782, 83, 84TRANSFECTION_PLATE_6G 82 - 84Neuro2a - PVI

29pCMV_148H7RGS18_SNP5196_1F_SmaIENH_4053050AJ85, 86, 87TRANSFECTION_PLATE_6H 85 - 87Neuro2a - PVI

30pCMV_149B5RGS18_SNP5196_1F_SmaIENH_4053050C5788, 89, 90TRANSFECTION_PLATE_6H 88 - 90Neuro2a - PVI

31pCMV_146B2RGS18_SNP5196_1F_SmaIENH_2930303AJ91, 92, 93TRANSFECTION_PLATE_6H 91 - 93Neuro2a - PVI

32pCMV_151B8RGS18_SNP5196_1F_SmaIENH_2930303C5794, 95, 96TRANSFECTION_PLATE_6H 94 - 96Neuro2a - PVI

33pCMV_132E8RGS18_SNP5196_1F_SmaIENH_2413450AJ97, 98, 99TRANSFECTION_PLATE_7A 1 - 3Neuro2a - PVI

34pCMV_134B8RGS18_SNP5196_1F_SmaIENH_2413450C57100, 101, 102TRANSFECTION_PLATE_7A 4 - 6Neuro2a - PVI

35pCMV_132D9RGS18_SNP5196_1F_SmaIENH_2415436AJ103, 104, 105TRANSFECTION_PLATE_7A 7 - 9Neuro2a - PVI

36pCMV_134H10RGS18_SNP5196_1F_SmaIENH_2415436C57106, 107, 108TRANSFECTION_PLATE_7A 10 - 12Neuro2a - PVI

37pCMV_13C10BMP_prom_ex1_1AJ109, 110, 111TRANSFECTION_PLATE_7B 13 - 15Neuro2a - PVI

38pCMV_124C11BMP_prom_ex1_1C57112, 113, 114TRANSFECTION_PLATE_7B 16 - 18Neuro2a - PVI

39pCMV_111G7RGS1SNP2885_2FAJ115, 116, 117TRANSFECTION_PLATE_7B 19 - 21Neuro2a - PVI

40pCMV_152G9RGS1SNP2885_2FC57118, 119, 120TRANSFECTION_PLATE_7B 22 - 24Neuro2a - PVI

41pCMV_129F2RGS1SNP2885_2FRIII121, 122, 123TRANSFECTION_PLATE_7C 25 - 27Neuro2a - PVI

42pCMV_1AJ_2RGS18_SNP5196 (binnaz)AJ124, 125, 126TRANSFECTION_PLATE_7C 28 - 30Neuro2a - PVI

43pCMV_1C57_2RGS18_SNP5196 (binnaz)C57127, 128, 129TRANSFECTION_PLATE_7C 31 - 33Neuro2a - PVI

44pCMV_156G9RGS1_5prime2FC57130, 131, 132TRANSFECTION_PLATE_7C 34 - 36Neuro2a - PVI

45pCMV_148C1RGS18_SNP5196_1F_SmaIENH_2411196AJ133, 134, 135TRANSFECTION_PLATE_7D 37 - 39Neuro2a - PVI

46pCMV_1MUTS140G136, 137, 138TRANSFECTION_PLATE_7D 40 - 42Neuro2a - PVI

47pCMV_1WTCONTROL139, 140, 141TRANSFECTION_PLATE_7D 43 - 45Neuro2a - PVI

48cells only blank142, 143, 144TRANSFECTION_PLATE_7D 46 - 48Neuro2a - PVI

49pCMV_165A8RGS18_SNP5196_1F_SmaIENH_2685395AJ145, 146, 147TRANSFECTION_PLATE_7E 49 - 51Neuro2a - PVI

50pCMV_165C11RGS18_SNP5196_1F_SmaIENH_2685395C57148, 149, 150TRANSFECTION_PLATE_7E 52 - 54Neuro2a - PVI

51pCMV_165G4RGS18_SNP5196_1F_SmaIENH_2418850AJ151, 152, 153TRANSFECTION_PLATE_7E 55 - 57Neuro2a - PVI

52pCMV_133F3RGS18_SNP5196_1F_SmaIENH_2418850C57154, 155, 156TRANSFECTION_PLATE_7E 58 - 60Neuro2a - PVI

53pCMV_165A2RGS18_SNP5196_1F_SmaIENH_3226837AJ157, 158, 159TRANSFECTION_PLATE_7F 61 - 63Neuro2a - PVI

54pCMV_173G1RGS18_SNP5196_1F_SmaIENH_3226837C57160, 161, 162TRANSFECTION_PLATE_7F 64 - 66Neuro2a - PVI

55pCMV_148E8RGS18_SNP5196_1F_SmaIENH_4107860AJ163, 164, 165TRANSFECTION_PLATE_7F 67 - 69Neuro2a - PVI

56pCMV_165C8RGS18_SNP5196_1F_SmaIENH_4107860C57166, 167, 168TRANSFECTION_PLATE_7F 70 - 72Neuro2a - PVI

57pCMV_166D5BMP_prom_ex1_1F_SmaIAJ169, 170, 171TRANSFECTION_PLATE_7G 73 - 75Neuro2a - PVI

58pCMV_167H5BMP_prom_ex1_1F_SmaIC57172, 173, 174TRANSFECTION_PLATE_7G 76 - 78Neuro2a - PVI

59pCMV_167D9BMP_prom_ex1_1F_SmaIRIII175, 176, 177TRANSFECTION_PLATE_7G 79 - 81Neuro2a - PVI

60pCMV_158G3RGS2_Func8F_5RAJ178, 179, 180TRANSFECTION_PLATE_7G 82 - 84Neuro2a - PVI

61pCMV_170E6RGS2_Func8F_5RC57181, 182, 183TRANSFECTION_PLATE_7H 85 - 87Neuro2a - PVI

62pCMV_168B1RGS1_5prime_2F_SmaIAJ184, 185, 186TRANSFECTION_PLATE_7H 88 - 90Neuro2a - PVI

63pCMV_166A9RGS1_5prime_2F_SmaIC57187, 188, 189TRANSFECTION_PLATE_7H 91 - 93Neuro2a - PVI

64pCMV_167A2RGS1_5prime_2F_SmaIRIII190, 191, 192TRANSFECTION_PLATE_7H 94 - 96Neuro2a - PVI

1pCMV_1pGL3_control1, 2, 3TRANSFECTION_PLATE_8A 1 - 3ND7/23 - PV

2pCMV_1pGL3_basic4, 5, 6TRANSFECTION_PLATE_8A 4 - 6ND7/23 - PV

3pCMV_13B1RGS18_SNP5196_1F_SmaIAJ7, 8, 9TRANSFECTION_PLATE_8A 7 - 9ND7/23 - PV

4pCMV_16G10RGS18_SNP5196_1F_SmaIC5710, 11, 12TRANSFECTION_PLATE_8A 10 - 12ND7/23 - PV

5pCMV_150A1RGS18_SNP5196_1F_SmaIENH_3110794AJ13, 14, 15TRANSFECTION_PLATE_8B 13 - 15ND7/23 - PV

6pCMV_150E12RGS18_SNP5196_1F_SmaIENH_3110794C5716, 17, 18TRANSFECTION_PLATE_8B 16 - 18ND7/23 - PV

7pCMV_146A12RGS18_SNP5196_1F_SmaIENH_2532527AJ19, 20, 21TRANSFECTION_PLATE_8B 19 - 21ND7/23 - PV

8pCMV_146A11RGS18_SNP5196_1F_SmaIENH_2532527C5722, 23, 24TRANSFECTION_PLATE_8B 22 - 24ND7/23 - PV

9pCMV_145F1RGS18_SNP5196_1F_SmaIENH_2156454AJ25, 26, 27TRANSFECTION_PLATE_8C 25 - 27ND7/23 - PV

10pCMV_147E2RGS18_SNP5196_1F_SmaIENH_2156454C5728, 29, 30TRANSFECTION_PLATE_8C 28 - 30ND7/23 - PV

11pCMV_145F11RGS18_SNP5196_1F_SmaIENH_2543708AJ31, 32, 33TRANSFECTION_PLATE_8C 31 - 33ND7/23 - PV

12pCMV_147H9RGS18_SNP5196_1F_SmaIENH_2543708C5734, 35, 36TRANSFECTION_PLATE_8C 34 - 36ND7/23 - PV

13pCMV_145B7RGS18_SNP5196_1F_SmaIENH_2558562AJ37, 38, 39TRANSFECTION_PLATE_8D 37 - 39ND7/23 - PV

14pCMV_147E7RGS18_SNP5196_1F_SmaIENH_2558562C5740, 41, 42TRANSFECTION_PLATE_8D 40 - 42ND7/23 - PV

15pCMV_146G3RGS18_SNP5196_1F_SmaIENH_2682967AJ43, 44, 45TRANSFECTION_PLATE_8D 43 - 45ND7/23 - PV

16pCMV_148G9RGS18_SNP5196_1F_SmaIENH_2682967C5746, 47, 48TRANSFECTION_PLATE_8D 46 - 48ND7/23 - PV

17pCMV_146B6RGS18_SNP5196_1F_SmaIENH_2490240AJ49, 50, 51TRANSFECTION_PLATE_8E 49 - 51ND7/23 - PV

18pCMV_148H11RGS18_SNP5196_1F_SmaIENH_2490240C5752, 53, 54TRANSFECTION_PLATE_8E 52 - 54ND7/23 - PV

19pCMV_146A9RGS18_SNP5196_1F_SmaIENH_2499645AJ55, 56, 57TRANSFECTION_PLATE_8E 55 - 57ND7/23 - PV

20pCMV_149A1RGS18_SNP5196_1F_SmaIENH_2499645C5758, 59, 60TRANSFECTION_PLATE_8E 58 - 60ND7/23 - PV

21pCMV_132E3RGS18_SNP5196_1F_SmaIENH_2404529AJ61, 62, 63TRANSFECTION_PLATE_8F 61 - 63ND7/23 - PV

22pCMV_134D5RGS18_SNP5196_1F_SmaIENH_2404529C5764, 65, 66TRANSFECTION_PLATE_8F 64 - 66ND7/23 - PV

23pCMV_131C5RGS18_SNP5196_1F_SmaIENH_2409307AJ67, 68, 69TRANSFECTION_PLATE_8F 67 - 69ND7/23 - PV

24pCMV_134C6RGS18_SNP5196_1F_SmaIENH_2409307C5770, 71, 72TRANSFECTION_PLATE_8F 70 - 72ND7/23 - PV

25pCMV_132C10RGS18_SNP5196_1F_SmaIENH_2417081AJ73, 74, 75TRANSFECTION_PLATE_8G 73 - 75ND7/23 - PV

26pCMV_135G2RGS18_SNP5196_1F_SmaIENH_2417081C5776, 77, 78TRANSFECTION_PLATE_8G 76 - 78ND7/23 - PV

27pCMV_133F4RGS18_SNP5196_1F_SmaIENH_2833990AJ79, 80, 81TRANSFECTION_PLATE_8G 79 - 81ND7/23 - PV

28pCMV_136B3RGS18_SNP5196_1F_SmaIENH_2833990C5782, 83, 84TRANSFECTION_PLATE_8G 82 - 84ND7/23 - PV

29pCMV_148H7RGS18_SNP5196_1F_SmaIENH_4053050AJ85, 86, 87TRANSFECTION_PLATE_8H 85 - 87ND7/23 - PV

30pCMV_149B5RGS18_SNP5196_1F_SmaIENH_4053050C5788, 89, 90TRANSFECTION_PLATE_8H 88 - 90ND7/23 - PV

31pCMV_146B2RGS18_SNP5196_1F_SmaIENH_2930303AJ91, 92, 93TRANSFECTION_PLATE_8H 91 - 93ND7/23 - PV

32pCMV_151B8RGS18_SNP5196_1F_SmaIENH_2930303C5794, 95, 96TRANSFECTION_PLATE_8H 94 - 96ND7/23 - PV

33pCMV_132E8RGS18_SNP5196_1F_SmaIENH_2413450AJ97, 98, 99TRANSFECTION_PLATE_9A 1 - 3ND7/23 - PV

34pCMV_134B8RGS18_SNP5196_1F_SmaIENH_2413450C57100, 101, 102TRANSFECTION_PLATE_9A 4 - 6ND7/23 - PV

35pCMV_132D9RGS18_SNP5196_1F_SmaIENH_2415436AJ103, 104, 105TRANSFECTION_PLATE_9A 7 - 9ND7/23 - PV

36pCMV_134H10RGS18_SNP5196_1F_SmaIENH_2415436C57106, 107, 108TRANSFECTION_PLATE_9A 10 - 12ND7/23 - PV

37pCMV_13C10BMP_prom_ex1_1AJ109, 110, 111TRANSFECTION_PLATE_9B 13 - 15ND7/23 - PV

38pCMV_124C11BMP_prom_ex1_1C57112, 113, 114TRANSFECTION_PLATE_9B 16 - 18ND7/23 - PV

39pCMV_111G7RGS1SNP2885_2FAJ115, 116, 117TRANSFECTION_PLATE_9B 19 - 21ND7/23 - PV

40pCMV_152G9RGS1SNP2885_2FC57118, 119, 120TRANSFECTION_PLATE_9B 22 - 24ND7/23 - PV

41pCMV_129F2RGS1SNP2885_2FRIII121, 122, 123TRANSFECTION_PLATE_9C 25 - 27ND7/23 - PV

42pCMV_1AJ_2RGS18_SNP5196 (binnaz)AJ124, 125, 126TRANSFECTION_PLATE_9C 28 - 30ND7/23 - PV

43pCMV_1C57_2RGS18_SNP5196 (binnaz)C57127, 128, 129TRANSFECTION_PLATE_9C 31 - 33ND7/23 - PV

44pCMV_156G9RGS1_5prime2FC57130, 131, 132TRANSFECTION_PLATE_9C 34 - 36ND7/23 - PV

45pCMV_148C1RGS18_SNP5196_1F_SmaIENH_2411196AJ133, 134, 135TRANSFECTION_PLATE_9D 37 - 39ND7/23 - PV

46pCMV_132C10 (1.5ul = 450ng vector)RGS18_SNP5196_1F_SmaIENH_2417081AJ136, 137, 138TRANSFECTION_PLATE_9D 40 - 42ND7/23 - PV

47pCMV_145F1 (1.5ul = 216ng vector)RGS18_SNP5196_1F_SmaIENH_2156454AJ139, 140, 141TRANSFECTION_PLATE_9D 43 - 45ND7/23 - PV142 - 50ul media & fugene6

48cells only blankTRANSFECTION_PLATE_9D 46 - 48ND7/23 - PV143 - nothing added

49pCMV_165A8RGS18_SNP5196_1F_SmaIENH_2685395AJ145, 146, 147TRANSFECTION_PLATE_9E 49 - 51ND7/23 - PV144 - 50ul media & fugene6 + 1.5ug pCMV_1

50pCMV_165C11RGS18_SNP5196_1F_SmaIENH_2685395C57148, 149, 150TRANSFECTION_PLATE_9E 52 - 54ND7/23 - PV

51pCMV_165G4RGS18_SNP5196_1F_SmaIENH_2418850AJ151, 152, 153TRANSFECTION_PLATE_9E 55 - 57ND7/23 - PV

52pCMV_133F3RGS18_SNP5196_1F_SmaIENH_2418850C57154, 155, 156TRANSFECTION_PLATE_9E 58 - 60ND7/23 - PV

53pCMV_165A2RGS18_SNP5196_1F_SmaIENH_3226837AJ157, 158, 159TRANSFECTION_PLATE_9F 61 - 63ND7/23 - PV

54pCMV_173G1RGS18_SNP5196_1F_SmaIENH_3226837C57160, 161, 162TRANSFECTION_PLATE_9F 64 - 66ND7/23 - PV

55pCMV_148E8RGS18_SNP5196_1F_SmaIENH_4107860AJ163, 164, 165TRANSFECTION_PLATE_9F 67 - 69ND7/23 - PV

56pCMV_165C8RGS18_SNP5196_1F_SmaIENH_4107860C57166, 167, 168TRANSFECTION_PLATE_9F 70 - 72ND7/23 - PV

57pCMV_166D5BMP_prom_ex1_1F_SmaIAJ169, 170, 171TRANSFECTION_PLATE_9G 73 - 75ND7/23 - PV

58pCMV_167H5BMP_prom_ex1_1F_SmaIC57172, 173, 174TRANSFECTION_PLATE_9G 76 - 78ND7/23 - PV

59pCMV_167D9BMP_prom_ex1_1F_SmaIRIII175, 176, 177TRANSFECTION_PLATE_9G 79 - 81ND7/23 - PV

60pCMV_158G3RGS2_Func8F_5RAJ178, 179, 180TRANSFECTION_PLATE_9G 82 - 84ND7/23 - PV

61pCMV_170E6RGS2_Func8F_5RC57TRANSFECTION_PLATE_9H 85 - 87ND7/23 - PVcells contaminated - orange media

62pCMV_168B1RGS1_5prime_2F_SmaIAJ184, 185, 186TRANSFECTION_PLATE_9H 88 - 90ND7/23 - PV

63pCMV_166A9RGS1_5prime_2F_SmaIC57187, 188, 189TRANSFECTION_PLATE_9H 91 - 93ND7/23 - PV

64pCMV_167A2RGS1_5prime_2F_SmaIRIII190, 191, 192TRANSFECTION_PLATE_9H 94 - 96ND7/23 - PV

1pCMV_1pGL3_control1, 2, 3TRANSFECTION_PLATE_10A 1 - 3ND7/23 - PVI

2pCMV_1pGL3_basic4, 5, 6TRANSFECTION_PLATE_10A 4 - 6ND7/23 - PVI

3pCMV_13B1RGS18_SNP5196_1F_SmaIAJ7, 8, 9TRANSFECTION_PLATE_10A 7 - 9ND7/23 - PVI

4pCMV_16G10RGS18_SNP5196_1F_SmaIC5710, 11, 12TRANSFECTION_PLATE_10A 10 - 12ND7/23 - PVI

5pCMV_150A1RGS18_SNP5196_1F_SmaIENH_3110794AJ13, 14, 15TRANSFECTION_PLATE_10B 13 - 15ND7/23 - PVI

6pCMV_150E12RGS18_SNP5196_1F_SmaIENH_3110794C5716, 17, 18TRANSFECTION_PLATE_10B 16 - 18ND7/23 - PVI

7pCMV_146A12RGS18_SNP5196_1F_SmaIENH_2532527AJ19, 20, 21TRANSFECTION_PLATE_10B 19 - 21ND7/23 - PVI

8pCMV_146A11RGS18_SNP5196_1F_SmaIENH_2532527C5722, 23, 24TRANSFECTION_PLATE_10B 22 - 24ND7/23 - PVI

9pCMV_145F1RGS18_SNP5196_1F_SmaIENH_2156454AJ25, 26, 27TRANSFECTION_PLATE_10C 25 - 27ND7/23 - PVI

10pCMV_147E2RGS18_SNP5196_1F_SmaIENH_2156454C5728, 29, 30TRANSFECTION_PLATE_10C 28 - 30ND7/23 - PVI

11pCMV_145F11RGS18_SNP5196_1F_SmaIENH_2543708AJ31, 32, 33TRANSFECTION_PLATE_10C 31 - 33ND7/23 - PVI

12pCMV_147H9RGS18_SNP5196_1F_SmaIENH_2543708C5734, 35, 36TRANSFECTION_PLATE_10C 34 - 36ND7/23 - PVI

13pCMV_145B7RGS18_SNP5196_1F_SmaIENH_2558562AJ37, 38, 39TRANSFECTION_PLATE_10D 37 - 39ND7/23 - PVI

14pCMV_147E7RGS18_SNP5196_1F_SmaIENH_2558562C5740, 41, 42TRANSFECTION_PLATE_10D 40 - 42ND7/23 - PVI

15pCMV_146G3RGS18_SNP5196_1F_SmaIENH_2682967AJ43, 44, 45TRANSFECTION_PLATE_10D 43 - 45ND7/23 - PVI

16pCMV_148G9RGS18_SNP5196_1F_SmaIENH_2682967C5746, 47, 48TRANSFECTION_PLATE_10D 46 - 48ND7/23 - PVI

17pCMV_146B6RGS18_SNP5196_1F_SmaIENH_2490240AJTRANSFECTION_PLATE_10E 49 - 51ND7/23 - PVIcells contaminated - orange media

18pCMV_148H11RGS18_SNP5196_1F_SmaIENH_2490240C5752, 53, 54TRANSFECTION_PLATE_10E 52 - 54ND7/23 - PVI

19pCMV_146A9RGS18_SNP5196_1F_SmaIENH_2499645AJ55, 56, 57TRANSFECTION_PLATE_10E 55 - 57ND7/23 - PVI

20pCMV_149A1RGS18_SNP5196_1F_SmaIENH_2499645C5758, 59, 60TRANSFECTION_PLATE_10E 58 - 60ND7/23 - PVI

21pCMV_132E3RGS18_SNP5196_1F_SmaIENH_2404529AJ61, 62, 63TRANSFECTION_PLATE_10F 61 - 63ND7/23 - PVI

22pCMV_134D5RGS18_SNP5196_1F_SmaIENH_2404529C5764, 65, 66TRANSFECTION_PLATE_10F 64 - 66ND7/23 - PVI

23pCMV_131C5RGS18_SNP5196_1F_SmaIENH_2409307AJ67, 68, 69TRANSFECTION_PLATE_10F 67 - 69ND7/23 - PVI

24pCMV_134C6RGS18_SNP5196_1F_SmaIENH_2409307C5770, 71, 72TRANSFECTION_PLATE_10F 70 - 72ND7/23 - PVI

25pCMV_132C10RGS18_SNP5196_1F_SmaIENH_2417081AJ73, 74, 75TRANSFECTION_PLATE_10G 73 - 75ND7/23 - PVI

26pCMV_135G2RGS18_SNP5196_1F_SmaIENH_2417081C5776, 77, 78TRANSFECTION_PLATE_10G 76 - 78ND7/23 - PVI

27pCMV_133F4RGS18_SNP5196_1F_SmaIENH_2833990AJ79, 80, 81TRANSFECTION_PLATE_10G 79 - 81ND7/23 - PVI

28pCMV_136B3RGS18_SNP5196_1F_SmaIENH_2833990C5782, 83, 84TRANSFECTION_PLATE_10G 82 - 84ND7/23 - PVI

29pCMV_148H7RGS18_SNP5196_1F_SmaIENH_4053050AJ85, 86, 87TRANSFECTION_PLATE_10H 85 - 87ND7/23 - PVI

30pCMV_149B5RGS18_SNP5196_1F_SmaIENH_4053050C5788, 89, 90TRANSFECTION_PLATE_10H 88 - 90ND7/23 - PVI

31pCMV_146B2RGS18_SNP5196_1F_SmaIENH_2930303AJ91, 92, 93TRANSFECTION_PLATE_10H 91 - 93ND7/23 - PVI

32pCMV_151B8RGS18_SNP5196_1F_SmaIENH_2930303C5794, 95, 96TRANSFECTION_PLATE_10H 94 - 96ND7/23 - PVI

33pCMV_132E8RGS18_SNP5196_1F_SmaIENH_2413450AJ97, 98, 99TRANSFECTION_PLATE_11A 1 - 3ND7/23 - PVI

34pCMV_134B8RGS18_SNP5196_1F_SmaIENH_2413450C57100, 101, 102TRANSFECTION_PLATE_11A 4 - 6ND7/23 - PVI

35pCMV_132D9RGS18_SNP5196_1F_SmaIENH_2415436AJ103, 104, 105TRANSFECTION_PLATE_11A 7 - 9ND7/23 - PVI

36pCMV_134H10RGS18_SNP5196_1F_SmaIENH_2415436C57106, 107, 108TRANSFECTION_PLATE_11A 10 - 12ND7/23 - PVI

37pCMV_13C10BMP_prom_ex1_1AJ109, 110, 111TRANSFECTION_PLATE_11B 13 - 15ND7/23 - PVI

38pCMV_124C11BMP_prom_ex1_1C57112, 113, 114TRANSFECTION_PLATE_11B 16 - 18ND7/23 - PVI

39pCMV_111G7RGS1SNP2885_2FAJ115, 116, 117TRANSFECTION_PLATE_11B 19 - 21ND7/23 - PVI

40pCMV_152G9RGS1SNP2885_2FC57118, 119, 120TRANSFECTION_PLATE_11B 22 - 24ND7/23 - PVI

41pCMV_129F2RGS1SNP2885_2FRIII121, 122, 123TRANSFECTION_PLATE_11C 25 - 27ND7/23 - PVI

42pCMV_1AJ_2RGS18_SNP5196 (binnaz)AJ124, 125, 126TRANSFECTION_PLATE_11C 28 - 30ND7/23 - PVI

43pCMV_1C57_2RGS18_SNP5196 (binnaz)C57127, 128, 129TRANSFECTION_PLATE_11C 31 - 33ND7/23 - PVI

44pCMV_156G9RGS1_5prime2FC57130, 131, 132TRANSFECTION_PLATE_11C 34 - 36ND7/23 - PVI

45pCMV_148C1RGS18_SNP5196_1F_SmaIENH_2411196AJ133, 134, 135TRANSFECTION_PLATE_11D 37 - 39ND7/23 - PVI

46pCMV_1+' fugene '+' DMEM136, 137, 138TRANSFECTION_PLATE_11D 40 - 42ND7/23 - PVI

47nothing added139, 140, 141TRANSFECTION_PLATE_11D 43 - 45ND7/23 - PVI

48cells only blank+' fugene '+' DMEM only142, 143, 144TRANSFECTION_PLATE_11D 46 - 48ND7/23 - PVI

49pCMV_165A8RGS18_SNP5196_1F_SmaIENH_2685395AJ145, 146, 147TRANSFECTION_PLATE_11E 49 - 51ND7/23 - PVI

50pCMV_165C11RGS18_SNP5196_1F_SmaIENH_2685395C57148, 149, 150TRANSFECTION_PLATE_11E 52 - 54ND7/23 - PVI

51pCMV_165G4RGS18_SNP5196_1F_SmaIENH_2418850AJ151, 152, 153TRANSFECTION_PLATE_11E 55 - 57ND7/23 - PVI

52pCMV_133F3RGS18_SNP5196_1F_SmaIENH_2418850C57154, 155, 156TRANSFECTION_PLATE_11E 58 - 60ND7/23 - PVI

53pCMV_165A2RGS18_SNP5196_1F_SmaIENH_3226837AJ157, 158, 159TRANSFECTION_PLATE_11F 61 - 63ND7/23 - PVI

54pCMV_173G1RGS18_SNP5196_1F_SmaIENH_3226837C57160, 161, 162TRANSFECTION_PLATE_11F 64 - 66ND7/23 - PVI

55pCMV_148E8RGS18_SNP5196_1F_SmaIENH_4107860AJ163, 164, 165TRANSFECTION_PLATE_11F 67 - 69ND7/23 - PVI

56pCMV_165C8RGS18_SNP5196_1F_SmaIENH_4107860C57166, 167, 168TRANSFECTION_PLATE_11F 70 - 72ND7/23 - PVI

57pCMV_166D5BMP_prom_ex1_1F_SmaIAJ169, 170, 171TRANSFECTION_PLATE_11G 73 - 75ND7/23 - PVI

58pCMV_167H5BMP_prom_ex1_1F_SmaIC57172, 173, 174TRANSFECTION_PLATE_11G 76 - 78ND7/23 - PVI

59pCMV_167D9BMP_prom_ex1_1F_SmaIRIII175, 176, 177TRANSFECTION_PLATE_11G 79 - 81ND7/23 - PVI

60pCMV_158G3RGS2_Func8F_5RAJ178, 179, 180TRANSFECTION_PLATE_11G 82 - 84ND7/23 - PVI

61pCMV_170E6RGS2_Func8F_5RC57181, 182, 183TRANSFECTION_PLATE_11H 85 - 87ND7/23 - PVI

62pCMV_168B1RGS1_5prime_2F_SmaIAJ184, 185, 186TRANSFECTION_PLATE_11H 88 - 90ND7/23 - PVI

63pCMV_166A9RGS1_5prime_2F_SmaIC57187, 188, 189TRANSFECTION_PLATE_11H 91 - 93ND7/23 - PVI

64pCMV_167A2RGS1_5prime_2F_SmaIRIII190, 191, 192TRANSFECTION_PLATE_11H 94 - 96ND7/23 - PVI

cotrasfec 1cotrasfec 2ul addedconversion to ng/ul= ul for 1.5ug

1pCMV_1pGL3_control1, 2, 3TRANSFECTION_PLATE_12A 1 - 3Neuro2a - PVIII

2pCMV_1pGL3_basic4, 5, 6TRANSFECTION_PLATE_12A 4 - 6Neuro2a - PVIII

3pCMV_165A8RGS18_SNP5196_1F_SmaIENH_2685395AJ7, 8, 9TRANSFECTION_PLATE_12A 7 - 9Neuro2a - PVIII

4pCMV_165C11RGS18_SNP5196_1F_SmaIENH_2685395C5710, 11, 12TRANSFECTION_PLATE_12A 10 - 12Neuro2a - PVIII

5pCMV_165G4RGS18_SNP5196_1F_SmaIENH_2418850AJ13, 14, 15TRANSFECTION_PLATE_12B 13 - 15Neuro2a - PVIII

6pCMV_133F3RGS18_SNP5196_1F_SmaIENH_2418850C5716, 17, 18TRANSFECTION_PLATE_12B 16 - 18Neuro2a - PVIII

7pCMV_165A2RGS18_SNP5196_1F_SmaIENH_3226837AJ19, 20, 21TRANSFECTION_PLATE_12B 19 - 21Neuro2a - PVIII

8pCMV_173G1RGS18_SNP5196_1F_SmaIENH_3226837C5722, 23, 24TRANSFECTION_PLATE_12B 22 - 24Neuro2a - PVIII

9pCMV_148E8RGS18_SNP5196_1F_SmaIENH_4107860AJ25, 26, 27TRANSFECTION_PLATE_12C 25 - 27Neuro2a - PVIII

10pCMV_165C8RGS18_SNP5196_1F_SmaIENH_4107860C5728, 29, 30TRANSFECTION_PLATE_12C 28 - 30Neuro2a - PVIII

11pCMV_166D5BMP_prom_ex1_1F_SmaIAJ31, 32, 33TRANSFECTION_PLATE_12C 31 - 33Neuro2a - PVIII

12pCMV_167H5BMP_prom_ex1_1F_SmaIC5734, 35, 36TRANSFECTION_PLATE_12C 34 - 36Neuro2a - PVIII

13pCMV_167D9BMP_prom_ex1_1F_SmaIRIII37, 38, 39TRANSFECTION_PLATE_12D 37 - 39Neuro2a - PVIII

14pCMV_158G3RGS2_Func8F_5RAJ40, 41, 42TRANSFECTION_PLATE_12D 40 - 42Neuro2a - PVIII

15pCMV_170E6RGS2_Func8F_5RC5743, 44, 45TRANSFECTION_PLATE_12D 43 - 45Neuro2a - PVIII

16pCMV_168B1RGS1_5prime_2F_SmaIAJ46, 47, 48TRANSFECTION_PLATE_12D 46 - 48Neuro2a - PVIII

17pCMV_166A9RGS1_5prime_2F_SmaIC5749, 50, 51TRANSFECTION_PLATE_12E 49 - 51Neuro2a - PVIII

18pCMV_167A2RGS1_5prime_2F_SmaIRIII52, 53, 54TRANSFECTION_PLATE_12E 52 - 54Neuro2a - PVIII

19pCMV_146B6RGS18_SNP5196_1F_SmaIENH_2490240AJ55, 56, 57TRANSFECTION_PLATE_12E 55 - 57Neuro2a - PVIII1523.28767123292.87

20pCMV_148H11RGS18_SNP5196_1F_SmaIENH_2490240C5758, 59, 60TRANSFECTION_PLATE_12E 58 - 60Neuro2a - PVIII1382.53424657533.92

21pCMV_146A9RGS18_SNP5196_1F_SmaIENH_2499645AJ61, 62, 63TRANSFECTION_PLATE_12F 61 - 63Neuro2a - PVIII1128.424657534211.68

22pCMV_149A1RGS18_SNP5196_1F_SmaIENH_2499645C5764, 65, 66TRANSFECTION_PLATE_12F 64 - 66Neuro2a - PVIII1502.73972602742.98

23pCMV_132C10RGS18_SNP5196_1F_SmaIENH_2417081AJ67, 68, 69TRANSFECTION_PLATE_12F 67 - 69Neuro2a - PVIII3.33190.582191780826.21

24pCMV_135G2RGS18_SNP5196_1F_SmaIENH_2417081C5770, 71, 72TRANSFECTION_PLATE_12F 70 - 72Neuro2a - PVIII1893.83561643841.68

25pCMV_132D9RGS18_SNP5196_1F_SmaIENH_2415436AJ73, 74, 75TRANSFECTION_PLATE_12G 73 - 75Neuro2a - PVIII1382.53424657533.92

26pCMV_134H10RGS18_SNP5196_1F_SmaIENH_2415436C5776, 77, 78TRANSFECTION_PLATE_12G 76 - 78Neuro2a - PVIII1456.33561643843.29

27blank79, 80, 81TRANSFECTION_PLATE_12G 79 - 81Neuro2a - PVIII

28pCMV_148H7RGS18_SNP5196_1F_SmaIENH_4053050AJ82, 83, 84TRANSFECTION_PLATE_12G 82 - 84Neuro2a - PVIII1497.08904109593.02

29pCMV_149B5RGS18_SNP5196_1F_SmaIENH_4053050C5785, 86, 87TRANSFECTION_PLATE_12H 85 - 87Neuro2a - PVIII1610.61643835622.46

30pCMV_150A1RGS18_SNP5196_1F_SmaIENH_3110794AJ88, 89, 90TRANSFECTION_PLATE_12H 88 - 90Neuro2a - PVIII1378.42465753423.96

31pCMV_150E12RGS18_SNP5196_1F_SmaIENH_3110794C5791, 92, 93TRANSFECTION_PLATE_12H 91 - 93Neuro2a - PVIII1827.73972602741.81

32pCMV_1blank94, 95, 96TRANSFECTION_PLATE_12H 94 - 96Neuro2a - PVIII

B_gal

TRANSFECTION_PLATE_1

123456789101112

A0.1030.0980.1080.1610.1770.1590.1520.1670.180.1290.1170.121

B0.1650.1720.1850.1560.1740.1740.1730.1690.1860.210.2150.199

C0.1060.1120.1060.1220.1250.1350.1730.1890.1940.1550.1610.151

D0.1710.1750.1280.1750.1240.1380.110.1680.1820.1720.1830.183swapped D3 and D4, and added 180ul of D7 to D6

E0.1370.1480.1420.1560.1570.1540.0970.0790.0830.2440.4080.222

F0.1880.1940.1950.180.1560.180.2340.1970.2380.2060.2020.201

G0.0930.1080.1070.1970.1840.1850.1580.160.1650.1610.1520.175

H0.1730.1730.1750.2220.1970.2150.0560.0540.0510.050.050.05background average:0.0536666667


123456789101112

A0.090.1050.0920.10.1430.0930.10.2280.2320.3820.0840.098

B0.0920.0860.0860.0920.0940.0980.1090.1110.1060.1090.1070.115

C0.090.090.0920.0980.0940.0980.120.1260.1250.1070.1040.13

D0.1160.1110.1110.0970.0930.0930.1210.1130.1160.1050.0980.096

E0.0940.0850.090.0980.0960.1020.0750.0760.0740.1270.1220.124

F0.090.0970.0970.1090.1020.110.1020.0940.0960.0950.0890.101

G0.0890.0950.0980.0980.0890.0950.1050.0990.1080.1020.1020.106

H0.0930.10.0980.1130.1170.1260.1060.090.0740.080.0760.072


123456789101112

A0.1070.1040.1060.110.1190.1090.0960.1010.1010.1380.1240.128

B0.1010.1050.1030.120.1270.1280.120.1340.1250.1130.1120.104

C0.0740.0770.0790.0840.0810.0850.0830.0850.0810.0810.080.085

D0.0680.0680.0660.0990.0890.0970.080.0710.0710.0660.0720.072background average:0.07

E0.0470.0460.0480.0960.0930.1

F

G

H


123456789101112

A0.2990.2880.2790.2780.3270.3450.1890.2090.1960.1080.2630.106

B0.1940.1840.1580.0910.0890.0930.4010.5680.380.7230.5030.932

C0.2620.3020.2760.3750.3190.3410.4490.4120.4020.3350.4420.333

D0.1780.2790.1470.3560.3460.340.4590.4970.6850.2660.2720.252

E0.6220.640.6810.4890.5770.4260.4250.2550.2650.1690.3380.397

F0.1810.2070.2170.2840.3590.3020.350.2710.3370.2860.3330.373

G0.3460.3420.30.1340.2830.1250.3860.4240.3870.3770.3890.454

H0.4030.4460.3720.0930.0920.0930.2390.2010.260.1380.1250.167


123456789101112

A0.4820.4030.4090.4410.4670.3190.3320.3190.2910.3980.3390.472

B0.1910.1520.2060.4340.4570.4880.2970.3060.2750.1280.140.143

C0.1590.210.2160.3450.3130.4170.2020.2380.2690.2170.2570.318

D0.3250.3590.3990.2760.2840.380.3250.4620.5610.0670.0630.06450ul media & fugene6background average:0.0646666667

E0.0630.070.0750.0630.0640.0640.0780.0830.0790.0740.0780.074dropped 12well plate before transfection so inconsistent number of cells - didn't transfect row E.

F0.2920.4050.3570.210.1690.2830.7460.7890.760.5910.6450.742

G0.5390.670.6160.210.3310.2670.2250.2850.1960.1680.1910.219

H0.2570.4210.370.2130.3120.3050.1350.1520.1330.1010.0690.075dropped 12well plate after transfection so cross contamination likely for row H


123456789101112

A0.4170.2920.4240.5310.5670.5570.6250.6740.6270.3260.2940.228

B0.3790.4650.5090.7010.780.8540.6260.6550.5980.7360.7840.706

C0.5910.6280.7050.6450.6430.6550.9610.9520.9210.7190.7170.65

D0.9840.8820.8830.6120.5460.5360.8230.740.720.6110.7540.747

E0.8830.8040.9270.7130.6980.7750.5670.4590.5030.7260.7630.728

F0.7080.8140.7790.6480.690.6820.8120.7390.760.6470.6380.3

G0.1980.4310.460.2910.3960.3370.7560.7760.5340.1840.6970.695

H0.4210.3960.1620.3120.5360.6150.7820.7490.5920.3440.4490.556


123456789101112

A0.9090.920.8990.810.7770.8480.8560.8370.740.8540.9180.832

B0.8230.610.7750.9070.8660.9260.650.7380.6930.7080.7210.734

C0.5090.4170.4460.410.3760.4620.3540.3920.3910.5630.4710.467


E0.5040.4420.5070.4960.460.4780.6270.7220.6090.4240.4230.387dave - mutS140G

F0.3230.5490.6570.3750.2670.3390.930.8030.7470.4080.3830.399dave - wt CONTROL

G0.7660.7050.650.6620.7880.6980.4520.4810.3580.6080.5420.584

H0.5490.6350.4960.2890.3080.3160.3360.2990.3670.280.2770.256


123456789101112

A0.1910.1890.2120.1140.1640.1060.1310.1090.1260.0790.0720.065

B0.0790.1230.0830.090.0730.0660.0710.1080.0870.1710.1980.188

C0.10.1290.1160.1480.1850.1750.1540.1840.1610.2040.1710.286

D0.1440.1830.1860.1120.1060.1110.210.3280.260.1370.1530.142

E0.150.2230.1590.2240.1230.2090.230.1160.1410.1010.0720.13

F0.1160.0810.1090.0770.0780.110.1520.1040.1090.0920.1040.101

G0.1420.1970.2030.0660.0730.0580.0890.0650.0770.1280.1310.175

H0.2330.1480.1020.0720.080.070.1190.0990.1190.1140.0610.071


123456789101112

A0.1320.140.1410.1380.1090.1290.1070.1330.1370.2580.2420.247

B0.1390.110.0840.1570.1280.1650.1090.0990.1440.1470.150.137

C0.0810.0880.090.1240.1210.1010.1880.1580.1830.1450.1210.167142 - 50ul media & fugene6

D0.1350.1020.1340.3580.4650.3790.3450.4650.4250.0580.0620.367143 - nothing addedbackground average:0.058

E0.1310.1250.1310.0930.1680.0950.1470.1980.1150.1550.1270.127144 - 50ul media & fugene6 + 1.5ug pCMV_1

F0.1920.2120.1750.2360.1470.1280.160.1730.0850.1230.1130.101

G0.2220.1960.1190.090.1380.0860.1370.1390.1170.1110.1330.089

H0.0830.2610.1720.1130.0870.0750.0850.0890.0750.2110.0620.065cells contaminated - orange media


123456789101112

A0.9931.0230.9840.2480.2150.230.7090.6840.5070.6720.3150.391

B0.8830.7740.6360.2120.2060.2070.6540.5890.5020.3710.530.61

C0.7890.7260.7360.6130.6170.6230.8540.8960.520.8570.8670.841

D0.7270.750.5790.3910.3560.4790.4080.5130.4650.3030.4380.494

E0.10.6590.3790.8080.8370.5640.9080.9120.9340.740.8050.816

F0.4840.6350.4810.5430.6190.5680.5020.520.4190.2540.290.337

G0.3190.3270.3450.0980.460.2330.1060.1110.1440.1510.1380.208

H0.310.380.3010.1630.210.1880.1410.1440.1140.6420.3650.26


123456789101112

A0.2150.2320.2040.2730.2090.2150.2040.1750.1390.1020.0750.104

B0.1480.2120.2110.4670.3890.4110.1560.1360.1410.1740.2030.191

C0.1030.1120.1580.2140.3790.360.1970.1290.1730.3010.2610.48+' fugene '+' DMEM

D0.2450.2060.2530.8750.870.880.080.0660.0610.0640.0670.075nothing addedbackground average:0.0686666667

E0.1570.1530.1260.3660.2710.2680.1270.1220.1250.2790.2660.177+' fugene '+' DMEM only

F0.4750.440.5740.1860.1410.1280.510.7740.5360.1740.1570.1

G0.1230.2480.1450.1420.1070.1630.230.1760.1380.8080.5020.711

H0.1130.1350.1460.0960.1040.1220.1080.1620.0990.1280.1660.152


123456789101112

A0.2820.4040.4210.2070.1620.1960.2440.1130.3040.2690.1960.189

B0.1270.1890.1510.090.3230.3010.1220.1540.160.1090.3180.32

C0.3540.2780.390.1290.0840.1570.1830.1470.1030.2130.250.222

D0.0910.2640.1840.0830.1630.1150.1260.1350.080.1510.1380.102

E0.0790.1390.0920.1050.1080.3540.2250.1930.0650.0840.0650.086

F0.2340.170.1210.0650.0570.0660.0730.0520.0530.0620.0560.078

G0.0530.10.0490.0470.0480.0520.0390.0540.0540.0680.0650.069background average:0.049

H0.0620.0720.0580.0540.0960.0490.0820.0510.0560.1930.5720.098

B_gal_background_corrected


123456789101112

A0.0493330.0443330.0543330.1073330.1233330.1053330.0983330.1133330.1263330.0753330.0633330.067333

B0.1113330.1183330.1313330.1023330.1203330.1203330.1193330.1153330.1323330.1563330.1613330.145333

C0.0523330.0583330.0523330.0683330.0713330.0813330.1193330.1353330.1403330.1013330.1073330.097333


E0.0833330.0943330.0883330.1023330.1033330.1003330.0433330.0253330.0293330.1903330.3543330.168333

F0.1343330.1403330.1413330.1263330.1023330.1263330.1803330.1433330.1843330.1523330.1483330.147333

G0.0393330.0543330.0533330.1433330.1303330.1313330.1043330.1063330.1113330.1073330.0983330.121333

H0.1193330.1193330.1213330.1683330.1433330.1613330.0023330.000333-0.002667-0.003667-0.003667-0.003667


123456789101112

A0.020.0350.0220.030.0730.0230.030.1580.1620.3120.0140.028

B0.0220.0160.0160.0220.0240.0280.0390.0410.0360.0390.0370.045

C0.020.020.0220.0280.0240.0280.050.0560.0550.0370.0340.06

D0.0460.0410.0410.0270.0230.0230.0510.0430.0460.0350.0280.026

E0.0240.0150.020.0280.0260.0320.0050.0060.0040.0570.0520.054

F0.020.0270.0270.0390.0320.040.0320.0240.0260.0250.0190.031

G0.0190.0250.0280.0280.0190.0250.0350.0290.0380.0320.0320.036

H0.0230.030.0280.0430.0470.0560.0360.020.0040.010.0060.002


123456789101112

A0.0370.0340.0360.040.0490.0390.0260.0310.0310.0680.0540.058

B0.0310.0350.0330.050.0570.0580.050.0640.0550.0430.0420.034

C0.0040.0070.0090.0140.0110.0150.0130.0150.0110.0110.010.015

D-0.002-0.002-0.0040.0290.0190.0270.010.0010.001-0.0040.0020.002

E-0.023-0.024-0.0220.0260.0230.03

F

G

H


123456789101112

A0.2343330.2233330.2143330.2133330.2623330.2803330.1243330.1443330.1313330.0433330.1983330.041333

B0.1293330.1193330.0933330.0263330.0243330.0283330.3363330.5033330.3153330.6583330.4383330.867333

C0.1973330.2373330.2113330.3103330.2543330.2763330.3843330.3473330.3373330.2703330.3773330.268333

D0.1133330.2143330.0823330.2913330.2813330.2753330.3943330.4323330.6203330.2013330.2073330.187333

E0.5573330.5753330.6163330.4243330.5123330.3613330.3603330.1903330.2003330.1043330.2733330.332333

F0.1163330.1423330.1523330.2193330.2943330.2373330.2853330.2063330.2723330.2213330.2683330.308333

G0.2813330.2773330.2353330.0693330.2183330.0603330.3213330.3593330.3223330.3123330.3243330.389333

H0.3383330.3813330.3073330.0283330.0273330.0283330.1743330.1363330.1953330.0733330.0603330.102333


123456789101112

A0.4173330.3383330.3443330.3763330.4023330.2543330.2673330.2543330.2263330.3333330.2743330.407333

B0.1263330.0873330.1413330.3693330.3923330.4233330.2323330.2413330.2103330.0633330.0753330.078333

C0.0943330.1453330.1513330.2803330.2483330.3523330.1373330.1733330.2043330.1523330.1923330.253333

D0.2603330.2943330.3343330.2113330.2193330.3153330.2603330.3973330.4963330.002333-0.001667-0.00066750ul media & fugene6

E-0.0016670.0053330.010333-0.001667-0.000667-0.0006670.0133330.0183330.0143330.0093330.0133330.009333dropped 12well plate before transfection so inconsistent number of cells - didn't transfect row E.

F0.2273330.3403330.2923330.1453330.1043330.2183330.6813330.7243330.6953330.5263330.5803330.677333

G0.4743330.6053330.5513330.1453330.2663330.2023330.1603330.2203330.1313330.1033330.1263330.154333



123456789101112

A0.3410.2160.3480.4550.4910.4810.5490.5980.5510.250.2180.152

B0.3030.3890.4330.6250.7040.7780.550.5790.5220.660.7080.63

C0.5150.5520.6290.5690.5670.5790.8850.8760.8450.6430.6410.574

D0.9080.8060.8070.5360.470.460.7470.6640.6440.5350.6780.671

E0.8070.7280.8510.6370.6220.6990.4910.3830.4270.650.6870.652

F0.6320.7380.7030.5720.6140.6060.7360.6630.6840.5710.5620.224

G0.1220.3550.3840.2150.320.2610.680.70.4580.1080.6210.619

H0.3450.320.0860.2360.460.5390.7060.6730.5160.2680.3730.48


123456789101112

A0.8330.8440.8230.7340.7010.7720.780.7610.6640.7780.8420.756

B0.7470.5340.6990.8310.790.850.5740.6620.6170.6320.6450.658

C0.4330.3410.370.3340.30.3860.2780.3160.3150.4870.3950.391

D0.1260.1120.1680.1050.0790.080.0790.1080.077-0.0020.002050ul media & fugene6

E0.4280.3660.4310.420.3840.4020.5510.6460.5330.3480.3470.311dave - mutS140G

F0.2470.4730.5810.2990.1910.2630.8540.7270.6710.3320.3070.323dave - wt CONTROL

G0.690.6290.5740.5860.7120.6220.3760.4050.2820.5320.4660.508

H0.4730.5590.420.2130.2320.240.260.2230.2910.2040.2010.18


123456789101112

A0.1330.1310.1540.0560.1060.0480.0730.0510.0680.0210.0140.007

B0.0210.0650.0250.0320.0150.0080.0130.050.0290.1130.140.13

C0.0420.0710.0580.090.1270.1170.0960.1260.1030.1460.1130.228

D0.0860.1250.1280.0540.0480.0530.1520.270.2020.0790.0950.084

E0.0920.1650.1010.1660.0650.1510.1720.0580.0830.0430.0140.072

F0.0580.0230.0510.0190.020.0520.0940.0460.0510.0340.0460.043

G0.0840.1390.1450.0080.0150.0010.0310.0070.0190.070.0730.117

H0.1750.090.0440.0140.0220.0120.0610.0410.0610.0560.0030.013


123456789101112

A0.0740.0820.0830.080.0510.0710.0490.0750.0790.20.1840.189

B0.0810.0520.0260.0990.070.1070.0510.0410.0860.0890.0920.079

C0.0230.030.0320.0660.0630.0430.130.10.1250.0870.0630.109142 - 50ul media & fugene6

D0.0770.0440.0760.30.4070.3210.2870.4070.36700.0040.309143 - nothing added


F0.1340.1540.1170.1780.0890.070.1020.1150.0270.0650.0550.043

G0.1640.1380.0610.0320.080.0280.0790.0810.0590.0530.0750.031



123456789101112

A0.9243330.9543330.9153330.1793330.1463330.1613330.6403330.6153330.4383330.6033330.2463330.322333

B0.8143330.7053330.5673330.1433330.1373330.1383330.5853330.5203330.4333330.3023330.4613330.541333

C0.7203330.6573330.6673330.5443330.5483330.5543330.7853330.8273330.4513330.7883330.7983330.772333

D0.6583330.6813330.5103330.3223330.2873330.4103330.3393330.4443330.3963330.2343330.3693330.425333

E0.0313330.5903330.3103330.7393330.7683330.4953330.8393330.8433330.8653330.6713330.7363330.747333

F0.4153330.5663330.4123330.4743330.5503330.4993330.4333330.4513330.3503330.1853330.2213330.268333

G0.2503330.2583330.2763330.0293330.3913330.1643330.0373330.0423330.0753330.0823330.0693330.139333

H0.2413330.3113330.2323330.0943330.1413330.1193330.0723330.0753330.0453330.5733330.2963330.191333


123456789101112

A0.1463330.1633330.1353330.2043330.1403330.1463330.1353330.1063330.0703330.0333330.0063330.035333

B0.0793330.1433330.1423330.3983330.3203330.3423330.0873330.0673330.0723330.1053330.1343330.122333

C0.0343330.0433330.0893330.1453330.3103330.2913330.1283330.0603330.1043330.2323330.1923330.411333+' fugene '+' DMEM

D0.1763330.1373330.1843330.8063330.8013330.8113330.011333-0.002667-0.007667-0.004667-0.0016670.006333nothing added


F0.4063330.3713330.5053330.1173330.0723330.0593330.4413330.7053330.4673330.1053330.0883330.031333

G0.0543330.1793330.0763330.0733330.0383330.0943330.1613330.1073330.0693330.7393330.4333330.642333

H0.0443330.0663330.0773330.0273330.0353330.0533330.0393330.0933330.0303330.0593330.0973330.083333


123456789101112

A0.2330.3550.3720.1580.1130.1470.1950.0640.2550.220.1470.14

B0.0780.140.1020.0410.2740.2520.0730.1050.1110.060.2690.271

C0.3050.2290.3410.080.0350.1080.1340.0980.0540.1640.2010.173

D0.0420.2150.1350.0340.1140.0660.0770.0860.0310.1020.0890.053

E0.030.090.0430.0560.0590.3050.1760.1440.0160.0350.0160.037

F0.1850.1210.0720.0160.0080.0170.0240.0030.0040.0130.0070.029

G0.0040.0510-0.002-0.0010.003-0.010.0050.0050.0190.0160.02

H0.0130.0230.0090.0050.04700.0330.0020.0070.1440.5230.049

Luciferase


123456789101112

A0.91081.0001.0940.03100.02410.02560.01240.01420.01400.01760.01340.0153

B0.01820.01830.02180.02030.01760.01900.01060.01010.01290.01470.01710.0150

C0.00080.00080.00130.00200.00200.00200.01150.01230.01100.01200.01480.0142


E0.00060.00050.00100.01740.01550.01390.00060.00010.00060.01400.01590.0112

F0.01280.01160.01180.01980.01880.01870.01160.00980.01700.01700.01950.0156

G0.00050.00100.00040.02340.02350.02150.00750.00780.01070.01610.01770.0155

H0.01010.01180.01060.01630.01790.02100.00020.00030.0005background average:0.0003


123456789101112

A0.26820.32570.37380.00900.00650.00700.00660.00450.00540.00350.00250.0038

B0.00340.00440.00510.00640.00440.00550.00590.00500.00680.00580.00450.0047

C-0.00010.00090.00090.00130.00090.00130.00550.00540.00500.00660.00590.0059

D0.00630.00660.00610.00780.00510.00630.00400.00340.00340.00750.00440.0061

E0.00070.00120.00140.00540.00560.00440.0004-0.00000.00040.00610.00400.0042

F0.00280.00360.00420.00870.00610.00720.00230.00270.00430.00350.00470.0048

G0.00040.00040.00040.00500.00590.00530.00560.00370.00450.00580.00540.0047

H0.00390.00480.00480.00800.00860.00990.00440.00350.00350.00430.00280.0026


123456789101112

A0.00270.00400.00340.00370.00320.00390.00030.00060.00050.00670.00580.0059

B0.00050.00140.00150.00140.00150.00150.00680.00600.00630.01080.01070.0088

C0.00530.00550.00580.00280.00330.00380.00320.00280.00260.01610.01370.0157

D0.00050.00100.00080.00300.00270.00210.00270.00140.00200.00040.00010.0002background average:0.0002

E0.00040.00030.0002

F

G

H


123456789101112

A0.17130.45070.44210.07100.07290.08050.04170.04510.04200.03800.04590.0374

B0.02780.02820.03750.01740.01580.01910.18770.08540.11180.10680.07420.1159

C0.00880.00890.00990.02250.01860.02090.12250.09250.09680.09530.11370.0940

D0.02120.03930.02350.11630.11900.13230.10000.10360.08670.07820.08840.0792

E0.00380.00500.00630.12060.15050.11140.00250.00250.00220.04500.16700.1262

F0.01860.02370.02130.03630.05020.04130.07710.06750.08340.14500.20110.1857

G0.00530.00750.00670.03920.04580.03280.07000.07050.04990.09310.09170.0811

H0.09920.10270.10040.03030.04230.03380.07700.06740.08390.05920.05640.0812


123456789101112

A0.06090.06770.07130.08200.08240.06840.00400.00360.00320.09610.06730.1166

B0.01710.01550.01950.02190.02300.02680.10420.11310.08720.05730.06380.0616

C0.04990.06500.05580.08570.09350.10970.05330.06400.08370.12370.14680.1998


E0.00080.00150.00110.00120.00090.00100.00080.00100.00150.00070.00030.0006dropped 12well plate before transfection so inconsistent number of cells - didn't transfect row E.

F0.08820.14050.10940.05480.03840.07020.00510.00730.00890.00780.00640.0068

G0.09870.12600.12510.05330.07690.06020.04920.06700.03540.04350.05780.0531



123456789101112

A0.50350.50120.70050.18490.15910.16670.22090.18160.17970.18890.18010.1349

B0.06300.09120.08970.16640.21180.23380.13680.15110.15090.16900.17980.1575

C0.01370.01800.01900.03490.03580.03150.25270.22400.24420.31900.27180.2475

D0.17050.21160.17570.13430.07220.13280.14340.11470.14780.19660.20850.2069

E0.00930.00610.01810.12940.12350.15770.00700.00650.00720.15150.14580.1535

F0.06130.08390.06650.10160.10200.08950.19110.20570.25210.36390.33660.1443

G0.00420.00630.01080.02630.04520.02440.10270.09590.07180.03220.18410.1772

H0.18780.16400.03420.13130.19810.25760.25390.21370.15120.18310.21720.3053


123456789101112

A0.12460.12020.14080.14480.13660.15270.00740.00680.00580.23430.24980.2245

B0.06600.06800.05970.06790.06760.08070.23030.29050.31040.16780.23740.2681

C0.14820.12900.14850.07690.06900.10550.10380.14320.13280.25900.21890.2318


E0.02940.04230.03940.05810.06050.06900.21450.20710.16940.03730.03800.0356dave - mutS140G

F0.02670.07790.10380.10180.07460.11410.36190.30620.27670.05450.05870.0773dave - wt CONTROL

G0.19620.17510.22050.04360.06400.05080.05410.06220.04790.46350.54080.4222

H0.05060.05310.04320.08160.10720.11270.04140.03690.05760.06500.08970.0773


123456789101112

A0.01340.01160.01990.00810.00610.01140.01240.00640.01320.01550.01240.0074

B0.00470.00800.00710.00930.00690.00690.00680.00850.00560.01720.01040.0257

C0.00100.00150.00120.00100.00270.00190.00910.01490.00780.01910.01380.0393

D0.01000.01520.01660.00880.00950.00720.00890.02150.01740.01920.01320.0202

E0.00180.00420.00270.01440.00950.01570.00140.00060.00110.00590.00280.0099

F0.00400.00190.00390.00410.00300.00710.00680.00680.00930.01370.01840.0152

G0.00110.00190.00130.00480.00300.00270.00890.00510.00860.01790.02120.0276

H0.00920.00810.00650.00420.00630.00310.01340.00780.00830.01220.00190.0099


123456789101112

A0.00310.00480.00630.00870.00710.01090.00110.00080.00100.01210.01440.0136

B0.00230.00150.00200.00250.00240.00340.01250.00760.01110.01850.01510.0152

C0.00430.00710.00630.00880.00700.00600.01310.01170.01430.01110.01040.0207142 - 50ul media & fugene6

D0.00380.00380.00570.00170.00150.00190.00160.00180.00150.00060.00040.0003143 - nothing addedbackground average:0.0006


F0.00660.00920.00650.01680.00790.00910.01130.01240.00440.00490.00650.0067

G0.01090.01140.00690.00180.00340.00110.00630.00840.00940.00550.03030.0112



123456789101112

A0.04830.07240.06290.01180.01130.01030.02920.02620.02110.04840.02210.0222

B0.02150.02240.01760.01200.01140.01190.03790.02910.02550.02120.02210.0300

C0.00860.00460.00490.00740.00780.00980.09270.06510.01700.17910.13250.1597

D0.02440.01930.01620.01480.01900.02720.01960.02320.01830.02090.03270.0380

E0.00050.00580.00530.03280.03180.02200.00250.00200.00130.03270.03490.0412

F0.00620.00940.01010.01370.01550.02120.04060.06230.04270.01940.01920.0303

G0.00180.00220.00240.00150.01320.00560.00570.00500.00530.00770.00950.0133

H0.00730.00960.00980.00770.00920.00990.01070.00910.00830.04970.02130.0185


123456789101112

A0.00160.00980.00680.01180.01350.01160.00060.00080.00090.00490.00270.0047

B0.00230.00510.00410.00700.00700.00810.01040.00690.00930.02640.03300.0269

C0.01110.01610.01160.00950.01810.01880.01850.01050.01500.02490.01420.0310+' fugene '+' DMEM

D0.00400.00560.00560.00040.00040.00050.00070.00030.00040.00020.00030.0006nothing addedbackground average:0.0003


F0.00800.01010.01460.01090.01070.00760.01140.02590.01810.00840.00900.0033

G0.00550.00820.01020.00230.00220.00290.01560.01130.00900.12360.08040.0865

H0.00190.00400.00370.00500.00610.01460.01120.01150.00790.00270.01820.0146


123456789101112

A0.11580.25690.29250.03150.02930.02900.01490.00370.01870.01800.01330.0130

B0.01800.03450.02610.00140.01280.01020.01030.01770.02070.01120.08720.0634

C0.03600.02960.06280.01150.00230.01280.03980.04080.01140.00810.00920.0068

D0.00270.01420.00910.00740.02700.01620.00730.00950.00380.02870.02100.0079

E0.00110.00690.00770.01110.00860.07970.00290.00290.00010.00600.00680.0023

F0.00090.00110.00070.00620.00120.00090.00010.0003-0.00000.00690.00210.0093

G0.00050.00080.00070.00080.00110.00240.00030.00040.00020.00530.00460.0028background average:0.0003

H0.00290.00870.00130.00080.00120.00200.00680.00060.00020.00000.00010.0005

Luc_background_corrected


123456789101112

A0.91051.00001.09410.03070.02380.02530.01210.01390.01370.01730.01310.0150

B0.01790.01800.02150.02000.01730.01870.01030.00980.01260.01440.01680.0147

C0.00050.00050.00100.00170.00170.00170.01120.01200.01070.01170.01450.0139


E0.00030.00020.00070.01710.01520.01360.0003-0.00020.00030.01370.01560.0109

F0.01250.01130.01150.01950.01850.01840.01130.00950.01670.01670.01920.0153

G0.00020.00070.00010.02310.02320.02120.00720.00750.01040.01580.01740.0152

H0.00980.01150.01030.01600.01760.0207-0.0001-0.00000.0002-0.0003-0.0003-0.0003


123456789101112

A0.26800.32550.37360.00880.00630.00680.00640.00430.00520.00330.00230.0036

B0.00320.00420.00490.00620.00420.00530.00570.00480.00660.00560.00430.0045

C-0.00030.00070.00070.00110.00070.00110.00530.00520.00480.00640.00570.0057

D0.00610.00640.00590.00760.00490.00610.00380.00320.00320.00730.00420.0059

E0.00050.00100.00120.00520.00540.00420.0002-0.00020.00020.00590.00380.0040

F0.00260.00340.00400.00850.00590.00700.00210.00250.00410.00330.00450.0046

G0.00020.00020.00020.00480.00570.00510.00540.00350.00430.00560.00520.0045

H0.00370.00460.00460.00780.00840.00970.00420.00330.00330.00410.00260.0024


123456789101112

A0.00250.00380.00320.00350.00300.00370.00010.00040.00030.00650.00560.0057

B0.00030.00120.00130.00120.00130.00130.00660.00580.00610.01060.01050.0086

C0.00510.00530.00560.00260.00310.00360.00300.00260.00240.01590.01350.0155

D0.00030.00080.00060.00280.00250.00190.00250.00120.00180.0002-0.0001-0.0000

E0.00020.00010.0000

F

G

H


123456789101112

A0.17060.45000.44140.07030.07220.07980.04100.04440.04130.03730.04520.0367

B0.02710.02750.03680.01670.01510.01840.18700.08470.11110.10610.07350.1152

C0.00810.00820.00920.02180.01790.02020.12180.09180.09610.09460.11300.0933

D0.02050.03860.02280.11560.11830.13160.09930.10290.08600.07750.08770.0785

E0.00310.00430.00560.11990.14980.11070.00180.00180.00150.04430.16630.1255

F0.01790.02300.02060.03560.04950.04060.07640.06680.08270.14430.20040.1850

G0.00460.00680.00600.03850.04510.03210.06930.06980.04920.09240.09100.0804

H0.09850.10200.09970.02960.04160.03310.07630.06670.08320.05850.05570.0805


123456789101112

A0.06020.06700.07060.08130.08170.06770.00330.00290.00250.09540.06660.1159

B0.01640.01480.01880.02120.02230.02610.10350.11240.08650.05660.06310.0609

C0.04920.06430.05510.08500.09280.10900.05260.06330.08300.12300.14610.1991

D0.09860.14240.16410.04080.04600.04860.08220.12490.18550.00020.0001-0.000350ul media & fugene6

E0.00010.00080.00040.00050.00020.00030.00010.00030.00080.0000-0.0004-0.0001dropped 12well plate before transfection so inconsistent number of cells - didn't transfect row E.

F0.08750.13980.10870.05410.03770.06950.00440.00660.00820.00710.00570.0061

G0.09800.12530.12440.05260.07620.05950.04850.06630.03470.04280.05710.0524

H0.02820.04800.04540.08510.12250.13160.00070.0001-0.0000-0.0001-0.0003-0.0002dropped 12well plate after transfection so cross contamination likely for row H


123456789101112

A0.50210.49980.69910.18350.15770.16530.21950.18020.17830.18750.17870.1335

B0.06160.08980.08830.16500.21040.23240.13540.14970.14950.16760.17840.1561

C0.01230.01660.01760.03350.03440.03010.25130.22260.24280.31760.27040.2461

D0.16910.21020.17430.13290.07080.13140.14200.11330.14640.19520.20710.2055

E0.00790.00470.01670.12800.12210.15630.00560.00510.00580.15010.14440.1521

F0.05990.08250.06510.10020.10060.08810.18970.20430.25070.36250.33520.1429

G0.00280.00490.00940.02490.04380.02300.10130.09450.07040.03080.18270.1758

H0.18640.16260.03280.12990.19670.25620.25250.21230.14980.18170.21580.3039


123456789101112

A0.12320.11880.13940.14340.13520.15130.00600.00540.00440.23290.24840.2231

B0.06460.06660.05830.06650.06620.07930.22890.28910.30900.16640.23600.2667

C0.14680.12760.14710.07550.06760.10410.10240.14180.13140.25760.21750.2304

D0.01370.01730.0270-0.0003-0.0007-0.0007-0.00070.0004-0.0001-0.00020.0004-0.000250ul media & fugene6

E0.02800.04090.03800.05670.05910.06760.21310.20570.16800.03590.03660.0342dave - mutS140G

F0.02530.07650.10240.10040.07320.11270.36050.30480.27530.05310.05730.0759dave - wt CONTROL

G0.19480.17370.21910.04220.06260.04940.05270.06080.04650.46210.53940.4208

H0.04920.05170.04180.08020.10580.11130.04000.03550.05620.06360.08830.0759


123456789101112

A0.01280.01100.01930.00750.00550.01080.01180.00580.01260.01490.01180.0068

B0.00410.00740.00650.00870.00630.00630.00620.00790.00500.01660.00980.0251

C0.00040.00090.00060.00040.00210.00130.00850.01430.00720.01850.01320.0387

D0.00940.01460.01600.00820.00890.00660.00830.02090.01680.01860.01260.0196

E0.00120.00360.00210.01380.00890.01510.00080.00000.00050.00530.00220.0093

F0.00340.00130.00330.00350.00240.00650.00620.00620.00870.01310.01780.0146

G0.00050.00130.00070.00420.00240.00210.00830.00450.00800.01730.02060.0270

H0.00860.00750.00590.00360.00570.00250.01280.00720.00770.01160.00130.0093


123456789101112

A0.00250.00420.00570.00810.00650.01030.00050.00020.00040.01150.01380.0130

B0.00170.00090.00140.00190.00180.00280.01190.00700.01050.01790.01450.0146

C0.00370.00650.00570.00820.00640.00540.01250.01110.01370.01050.00980.0201142 - 50ul media & fugene6

D0.00320.00320.00510.00110.00090.00130.00100.00120.0009-0.0000-0.0002-0.0003143 - nothing added


F0.00600.00860.00590.01620.00730.00850.01070.01180.00380.00430.00590.0061

G0.01030.01080.00630.00120.00280.00050.00570.00780.00880.00490.02970.0106



123456789101112

A0.04800.07210.06260.01150.01100.01000.02890.02590.02080.04810.02180.0219

B0.02120.02210.01730.01170.01110.01160.03760.02880.02520.02090.02180.0297

C0.00830.00430.00460.00710.00750.00950.09240.06480.01670.17880.13220.1594

D0.02410.01900.01590.01450.01870.02690.01930.02290.01800.02060.03240.0377

E0.00020.00550.00500.03250.03150.02170.00220.00170.00100.03240.03460.0409

F0.00590.00910.00980.01340.01520.02090.04030.06200.04240.01910.01890.0300

G0.00150.00190.00210.00120.01290.00530.00540.00470.00500.00740.00920.0130

H0.00700.00930.00950.00740.00890.00960.01040.00880.00800.04940.02100.0182


123456789101112

A0.00130.00950.00650.01150.01320.01130.00030.00050.00060.00460.00240.0044

B0.00200.00480.00380.00670.00670.00780.01010.00660.00900.02610.03270.0266

C0.01080.01580.01130.00920.01780.01850.01820.01020.01470.02460.01390.0307+' fugene '+' DMEM

D0.00370.00530.00530.00010.00010.00020.0004-0.00000.0001-0.0001-0.00000.0003nothing added


F0.00770.00980.01430.01060.01040.00730.01110.02560.01780.00810.00870.0030

G0.00520.00790.00990.00200.00190.00260.01530.01100.00870.12330.08010.0862

H0.00160.00370.00340.00470.00580.01430.01090.01120.00760.00240.01790.0143


123456789101112

A0.11547340.2566010.29224080.031221970.029041710.028721960.014648960.003430760.018395360.017684630.013037560.01266706

B0.01765560.034232060.025816340.0011165350.012536690.009860040.009979310.017433130.020440130.010860820.086914950.06311912

C0.035697310.029332370.06246820.011206120.0019535130.012494940.039537970.04046510.011148190.0077582770.0088910490.006451124

D0.0024314540.013878330.0087696090.007079580.026708550.015926530.0070411830.0091810330.0034721280.028401280.020702720.007619671

E0.0008246610.0066316470.0074014180.010803030.0082702220.079435170.0026490320.002607092-0.00024722940.0057021330.0065477640.002009335

F0.00058590340.0008103450.0003806940.005896190.0008675640.0005945106-0.000150722-0.0000243918-0.00030268760.006577810.0017655710.008965513

G0.00023656030.00048280630.00035903120.00049884930.0007927420.0020743480.00001911970.0000638062-0.00012156570.0050368570.0042864430.00254417

H0.0025741930.0084364250.0009773780.0004928160.0009226750.0017417150.0065418430.0002635047-0.0001473264-0.0002642607-0.00020391320.0002454278

NIH3T3_results


123456789101112

A8.840101941710.204479591810.13098148150.1905516770.1345198870.1592170440.07933546050.08305173650.07584861110.1342461240.111700.1236370248

B0.10867933330.1045504070.11612578380.12811551280.0996760920.10767879310.05944838150.05782508880.06776537630.06834309520.078150.0736676382

C0.00456927920.00438881880.00898370750.01381074590.0139291760.01223339260.06494815030.06326158730.05509077320.07548174190.090270.0919435762

D0.07694479530.09946748570.10068453120.08429114290.13925177420.06302494930.01580609090.04077092260.04764029120.08167465120.071650.1062212022

E0.00209556060.00113923990.00503890140.10986365380.09691936310.08859220780.0031676639-0.00220568990.00406514220.05634770490.038250.0490907207

F0.06672095740.05806432990.05899784620.10820755560.11840910260.10194627780.04833047010.04799079190.07035773110.08105218450.094980.0762983582

G0.0021772290.00672507410.00124527010.11730086290.12594929350.11458318920.04586262030.0467163750.06325133330.09835633540.114630.0870888

H0.05675757230.06628815030.05876182860.07202355860.08939832490.0964221395-0.0009025107-0.00066675740.0031333824-0.006-0.00600-0.006


123456789101112

A13.4002359.300945714316.98228181820.2935860.08609416440.29365478260.21296216670.02751433540.03186277160.01046124680.163820.1284793571

B0.14460340910.259586750.304014750.28256290910.1737378750.18967228570.14731384620.11635997560.18446877780.14283420510.115030.0989317556

C-0.0144319420.034900030.02996087730.03764207140.02902942920.03978746430.106741520.09339560710.0865120.17381970270.166540.0944837167

D0.13246406520.15602746340.14351526830.28223977780.21182526090.2657280.07363492160.07508951160.06909904350.20878354290.148230.2251122308

E0.02039131250.06975473330.05799050.18598453570.20858911540.13142646880.04849754-0.035027770.051317950.1036986140.072360.0743982222

F0.129144450.12604125930.14654581480.21906005130.18568356250.1745583250.06483968750.10211408330.15875373080.132175040.237830.1480483871

G0.01065758950.0067163640.00681893570.17136207140.29965847370.204484320.15499417140.1206180.11253952630.17415968750.161770.1241467778

H0.15957343480.15198010.16606589290.18054025580.17899202130.17257842860.11781863890.16314580.836272250.41421930.426961.195579


123456789101112

A0.06736610810.11235367650.08873980560.088084650.0613835510.09575943590.00245709620.01285699350.01025598060.09581094120.103380.0977135

B0.01084753550.03356477140.03975036360.024710340.02358005260.02294910340.132580480.09011092190.11114554550.24645534880.249810.2526654118

C1.283995750.7571710.62444133330.18741635710.27953390910.23774526670.23354476920.17071533330.22239572731.44746363641.354371.03431

D-0.12642075-0.416619-0.14016020.09632896550.12902847370.07097074070.24802921.1913611.798559-0.054699425-0.05454-0.00370625

E-0.0086008565-0.00257875-0.0019913364000

F

G

H

data divided by DNA concentration:


123456789101112

A15.592327188517.998840469617.86920321060.19668315320.13884840040.16434025010.0703437470.07363882810.06725208970.14752043740.12274926350.1358623059123456

B0.19145921770.18418533240.20457754970.1031850220.08027973760.08672516230.07964637490.07747155730.09078912550.06399289340.07317460710.0689785164789

C0.02290522810.02200060210.04503420780.0667119570.06728402630.05909264910.08393387870.08175429740.07119498020.14812277320.17713737890.1804269102

D0.22423034170.28986532760.29341200720.25739099310.42521848960.19245265560.02841107140.07328476090.08563228710.09241111360.08106843430.12018440785.52874391470.08787974070.0596402160.0579347820.15261890760.0551068594

E0.00266973690.00145138760.00641954280.19146634990.16890751430.15439525320.0164436954-0.01144998120.02110260470.07472084390.05071726060.06509759510.07069029750.02696343360.0914956365

F0.09169323250.07979660820.08107952030.10555952970.11551142720.09945147620.04907841160.04873347670.0714465570.10414626120.12204047270.0980379344

G0.01142409480.03528700140.00653403180.08748876620.09393919210.08546179120.06024239820.06136383940.08308317290.13264080330.1545849160.1174456973

H0.0761199270.08890177920.0788079180.07863480690.09760445270.1052730033-0.0015561448-0.00114964960.0054027024-0.0102907489-0.0102907489-0.0102907489


123456789101112

A23.635570030716.405171534829.95364717070.30303286280.08886445920.30310385870.18882548450.02439591880.02825151240.03194440850.50025407280.3923238932

B0.25992117850.46660099110.54645926130.2275779050.13992955320.15276322560.19736473080.15589407150.2471433040.13374246560.10770401570.0926345122

C-0.0723455290.17494950660.15019014870.18182770650.14022486880.19219089450.1379443410.12069713340.11180130120.34109780370.32681213470.1854115945

D0.38602302440.45469081150.41822812720.86184590970.6468285090.81142563140.13235701510.1349716060.12420388240.23622898190.1677110270.2547041418

E0.0259784610.08886728680.07387969460.32412703330.36352146640.22904523350.2517561188-0.1818330460.26639718040.1375113320.09595261690.0986570431

F0.17748054780.17321589690.20139488380.21369927330.18113956490.17028658110.06584311860.10369435690.16121053520.16983547490.30559474630.1902315909

G0.05592122420.0352412990.0357795010.12781028060.22350006210.15251448640.20359108440.15843659920.14782519880.23486723820.21815621410.1674211251

H0.21401053140.20382679620.22271783540.1971125620.19542221060.18841989580.20314757930.28130247181.44193384710.71043780090.73228769072.0505672129


123456789101112

A0.15476470530.25811768130.20386794250.14838304950.10340369730.16131161460.00573500510.03000896950.02393805430.27267166810.29421229250.278086226

B0.01200485260.0371457770.04399130640.03542179320.03380154820.03289709480.4517538760.30704337640.37871661770.38807617570.39335326380.3978547318

C2.57416243731.51798099551.25188375790.30857811620.46024823240.39144388280.97389622210.71189356430.92740402333.32428353763.11047532312.3754238928

D-0.2444376984-0.8055433106-0.27100327040.22791072490.30527653660.16791411470.73614130753.53591449835.3380552529000

E

F

G

Hcorrected for ug DNAcorrected for ug DNA

used after averagingused before averaging

vectorpromoterenhancerstrainplate Aposition Aplate Bposition BAVERAGESTDEVul addedpico ng/ulug usedAVERAGESTDEVAVERAGESTDEV

1pGL3_controlPLATE_1A 1 - 3PLATE_2A 1 - 311.47650425793.13453272295.50103.08219178080.566952054820.24245994155.528743914720.24245993425.5287439127

2pGL3_basicPLATE_1A 4 - 6PLATE_2A 4 - 60.19293725920.08514102271.50645.89041095890.96883561640.19914344180.08787974070.19914549740.0878806478

33B1RGS18_SNP5196_1F_SmaIAJPLATE_1A 7 - 9PLATE_2A 7 - 90.0850958470.0672637471.50751.88356164381.12782534250.07545126340.0596402160.07545126340.059640216

46G10RGS18_SNP5196_1F_SmaIC57PLATE_1A 10 - 12PLATE_2A 10 - 120.11205872120.05272164371.50606.67808219180.91001712330.12313913480.0579347820.22177573020.1818647849

550A1RGS18_SNP5196_1F_SmaIENH_3110794AJPLATE_1B 13 - 15PLATE_2B 13 - 150.17292673890.08663213671.50378.42465753420.56763698630.3046431840.15261890760.30886725510.1574370878

650E12RGS18_SNP5196_1F_SmaIENH_3110794C57PLATE_1B 16 - 18PLATE_2B 16 - 180.16357391130.0684212051.50827.73972602741.2416095890.13174343430.05510685940.13174343430.0551068594

746A12RGS18_SNP5196_1F_SmaIENH_2532527AJPLATE_1B 19 - 21PLATE_2B 19 - 210.1055302410.05276352861.50497.6027397260.74640410960.14138486070.07069029750.14138486070.0706902975

846A11RGS18_SNP5196_1F_SmaIENH_2532527C57PLATE_1B 22 - 24PLATE_2B 22 - 240.09615855780.0287963931.50711.98630136991.06797945210.09003783510.02696343360.09003783510.0269634336

945F1RGS18_SNP5196_1F_SmaIENH_2156454AJPLATE_1C 25 - 27PLATE_2C 25 - 270.01139512850.01825212616.9028.91105816950.19948630140.05712236070.09149563650.05712236070.0914956365

1047E2RGS18_SNP5196_1F_SmaIENH_2156454C57PLATE_1C 28 - 30PLATE_2C 28 - 300.02440537990.01267560456.8530.22197780220.20702054790.11788868370.06122872640.11788868370.0612287264

1145F11RGS18_SNP5196_1F_SmaIENH_2543708AJPLATE_1C 31 - 33PLATE_2C 31 - 330.07832493970.02023523951.17661.36869219060.77380136990.10122098860.02615043120.10122098860.0261504312

1247H9RGS18_SNP5196_1F_SmaIENH_2543708C57PLATE_1C 34 - 36PLATE_2C 34 - 360.11542264780.04299060941.18431.85511957280.50958904110.22650143250.08436329260.22650143250.0843632926

1345B7RGS18_SNP5196_1F_SmaIENH_2558562AJPLATE_1D 37 - 39PLATE_2D 37 - 390.11545170350.03296250641.50228.76712328770.34315068490.3364460820.09605840190.33840477090.0939327627

1447E7RGS18_SNP5196_1F_SmaIENH_2558562C57PLATE_1D 40 - 42PLATE_2D 40 - 420.19994586880.07870400631.50218.32191780820.32748287670.61055365960.24033014220.60774610940.2447967269

1546G3RGS18_SNP5196_1F_Sma

Zoology 2005 Part 2 Richard Mott. Inbred Mouse Strain Haplotype Structure When the genomes of a pair...

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Transcript of Zoology 2005 Part 2 Richard Mott. Inbred Mouse Strain Haplotype Structure When the genomes of a pair...