ZhongFCCMC Meeting Oct 24, 20061 Impact and Efficacy of Aerial ULV Application of Permanone 30-30...

zhong FCCMC Meeting Oct 24, 2006 1

Impact and Efficacy of Aerial ULV Application of Permanone 30-30

Research Report


Objectives: Measure permethrin residue ground deposition

Determine permethrin airborne concentration

Assess non-target impact using mosquito fish bioassay

Evaluate control efficacy against adult mosquitoes through bioassay

Correlate residue and bioassay


Materials and Methods

Study Site: Panama City Beach

12 stations (9 treatments and 3 controls)

The 9 treatment stations include 6 open sites and 3 in woodland area

Beach MCD’s helicopter (Helo N91336) equipped with WingmanTM GX and AIMMS-20 (Adapco) was used for the Aerial ULV spray at 150ft attitude.

Residue sample collections: yarn, water and filter paper

Permethrin Analysis -GC with ECD detector


Materials and Methods

Bioassay: caged mosquitoes (+50 female mosquitoes / cage) (Ochlerotatus taeniorhynchus) 12-hrs post-treatment

Bioassay: caged mosquito fish (Gambusia holbrooki) in plastic pans (10/pan). The acute toxicity was checked 1 hr after the spray and the chronic toxicity was assessed daily for 5 days in the same plastic pan (108-hrs post-treatment --worst case scenario)


BEACH MOSQUITO CONTROL DISTRICT AERIAL TREATMENT RECORD (Adulticide) PAGE:

DATE: 8/9/2006 AIRCRAFT: OH-58C N91336 PILOT: Jimmy Shehee

NURSE SITE - 1: PCB DISTRICT NURSE SITE - 2: None

HOUR METER OUT: 273.3 HOUR METER IN: 274.2 Total: 0.9

START TIME: 19:00:00 FINISH TIME: 23:00 INVERSION: NO

AIR TEMP: 83 HUMIDITY: Medium WIND : 265 8-10 kts

TREATMENT TREATED CHEMICAL-FORM. APPLICATION ADDITIONAL NOTES:

AREAS ACRES GALLONS POUNDS OUNCES RATE

Frank Brown 1965 10 PERMANONE 0.6 Oz/Ac Wind: 265/8-10 ALT: 200

1A-1 3296a

2A-1 1566a

3A-1 4584

4A-1 3620

5A-1 6161

TOTALS:

CREWS: VEHICLE # NAME: TIME IN: TIME OUT:

FLT. CREW 1 AIRCRAFT SHEHEE 1900 22:00

FLT. CREW 2

GRD. CREW 1 BASE LEE 1900 22:00

GRD. CREW 2 Asst PHILLIP 1900 22:00

GRD. CREW 3 Trng

GRD. CREW 4 TrngTotal Hours 9


Result (1) Permethrin Residue on Filter Paper (µg/m2) and (Fish Mortality)

Permethrin (ug/m2) on Filter Paper and Fish Mortality

Stations 6/1/06 6/20/06 6/27/06 7/18/06 8/9/06

1 117.71 (10%) 80.64 (20%) 89.39 (20%) 105.44 57.56

2 68.24 39.52 (30%) 44.43 (40%) 101.06 60.48

3 67.77 37 59.55(10%) 120.49 56.70(10%)

4 87 (10%) 84.15 68.1 96.55 78.32

5 125.93 91.58 (50%) 83.22 98.87(10%) 104.18(10%)

6 102.39 88.26(30%) 85.15 147.81 93.17

7 53.65 43.63(20%) 38.46 73.74 108.82(10%)

8 54.84 65.58(40%) 43.3(10%) 123.67 89.99(30%)

9 46.68 (10%) 49.27 43.04(20%) 109.48 150.99

10 ND ND(20%) ND ND(10%) ND

11 ND ND ND ND ND(20%)

12 ND (10%) ND(10%) ND(10%) ND(10%) ND(10%)

1 (water) NA NA 2.14ug/L 4.36ug/L NA


Result (2) Permethrin Residue on Yarn (µg/yarn) and (%Mosquito Mortality)

Permethrin (ug/yarn) on Yarn and Mosquito Mortality 50 female/cage

Stations 6/1/06 6/20/06 6/27/06 7/18/06 8/9/06

1 54.35 (80%) 20.58 (81%) 38.74 (81%) 21.60 (53%) 17.17 (71%)

2 14.78 (44%) 13.07(16%) 4.1 (16%) 8.55 (28%) 3.09 (4%)

3 13.57 (24%) 3.21 (35%) 27.53 (35%) 17.15 (52%) 41.15 (53%)

4 40.91 (100%) 35.16 (67%) 17.51 (67%) 5.62 (9%) 7.72 (37%)

5 170 (100%) 26 (59%) 61.86 (59%) 7.47 (6%) 23.27 (36%)

6 36.74 (98%) 45.3 (74%) 62.07 (74%) 9.36 (12%) 24.27 (74%)

7 2.04 (7%) 2.27 (11%) 1.45 (11%) 2.343 (0%) 7.94 (39%)

8 7.52 (27%) 4.23 (2%) 4.74 (2%) 3.55 (0%) 5.26 (23%)

9 3.70 (26%) 3.27 (8%) 2.98 (8%) 3.00 (0%) 6.08 (7%)

10 ND (4%) ND (4%) ND (4%) ND (0%) ND (7%)

11 ND (2%) ND (12%) ND (12%) ND (2%) ND (4%)

12 ND (4%) ND (10%) ND (10%) ND (0%) ND (9%)

13 NA (3%) NA (0%) NA (0%) NA (0%) NA (2%)


Discussion Permethrin Residue

Low level permethrin residue (2.14 ppb and 4.36 ppb) was detected in water samples from the fish tank from two separate spray events (6/27/2006 and 7/18/2006) and both samples collected from site 1

The highest permethrin deposition on filter paper was on site 9 (151µg/m2). That matched up to low residue on yarn (6.0 µg/yarn) indicating it may have the large droplets fall out. In fact that many of higher ground deposition (>90 µg/m2) did not have good mosquito control efficacy

The range of ground deposition was about 37 – 151 µg/m2

The highest permethrin residue found on yarn was 170 µg/m2 and the range was 3 – 170 µg/m2

With few exceptions, the residue on yarn reached >40 µg/yarn, the control efficacy was greatly improved.


Discussion (2) fish and mosquito assay

PPB level permethrin residue was detected in tank water samples from two separate spray events on site 1 did not cause fish mortality at higher concentration (4.36 ppb).

There were no indication of acute toxic effect permethrin on fish from field bioassay even with the highest ground deposition (151 µg/m2).

Various fish mortality found during each trial and the reason is unknown or

appears not correlated with permethrin concentration We suspect the stressed fish were picked for the assay in trial 2 and 3 as

the control fish mortality increasing. The fact of fish reservoir lost screen enclosure as a result from storm.

Only six stations in three spray evens reached >80% mosquito mortality. The low control efficacy in general a concern and the reasons need to be further investigated.


Acknowledgements The following personnel contributed to this project: Florida A&M University

C. Brock, T. Quimet, T. Lian, R. Aarons, N. Sickerman, -participation

K. Shaffer and J. Petersen –Provide mosquito pupae J. Smith, E. Cope and J. Walsh – Provide mosquito rearing room

Beach Mosquito Control Districts E. Hunter, J. Shehee, Skip, Lee, and Cindy - participation

Florida Department of Agricultural and Consumer Services Max Feken, James Clauson -participation

Bayer Environmental Science Peter Connelly -participation, Jing Zhai (consultant)

Adapco, Bill Reynolds –computer model and spray tech.

Lee County Mosquito Control District J. Hornby –droplets analysis

ZhongFCCMC Meeting Oct 24, 20061 Impact and Efficacy of Aerial ULV Application of Permanone 30-30...

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