Yield and Amino Acid Composition of Pulp and Protein ... · Vinni Kragbæk Damborg, Lene...

Yield and Amino Acid Composition of Pulp and Protein Extracted and

Recovered from Legumes and Grass Vinni Kragbæk Damborg, Lene Stødkilde, Søren Krogh

Jensen, Anders Peter S. Adamsen

European Federation of Animal Science, September 2015

Motivation

• Increasing demands of animal protein

• Sustainable, locally produced feed protein

• Soya bean protein

– Deforestation

– EU27 imports 20,000,000 tonnes annually

01-09-2015

EAAP 2015 Vinni Kragbæk Damborg

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Green Biomass

• Legumes and grasses – High availability in Northern Europe

– High yields

– High quality protein • High content

• Amino acid composition comparable to soya bean protein

• Nutritional value

– High content of water

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Study

• Purpose – Examination of extraction and recovery of protein

from locally grown plants

– Analysis of the recovered protein • Amino acid content

• Amino acid composition and distribution

– Most suitable plant species

– Most feasible method of recovery

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Materials and Methods • Material

– Three seasons • White clover • Red clover • Lucerne • Ryegrass

• Methods – Twin screw press – Two-step heat precipitation – Acidic precipitation

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Flow Chart

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Twin-screw press

Green Juice

Pulp Plant material

Green Juice pH

precipitation 3.0-5.0

Protein fraction

Brown Juice

Heat precipitation

60 °C Green Juice Green protein

Residue

Heat precipitation

80 °C White protein

Brown Juice

01-09-2015

Dry Matter Balance Dry Matter Plant Pulp Green

juice Loss

White clover 100g 55g 36g 9g

Red clover 100g 55g 30g 15g

Lucerne 100g 63g 28g 9g

Ryegrass 100g 68g 32g 0g

01-09-2015


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Green protein

White protein

Brown juice

15g <1g 20g

13g <1g 17g

13g <1g 15g

12g <1g 20g

Recovered by Heat Precipitation

Crude Protein Balance Crude Protein

Plant Pulp Green juice

Loss

White clover 100g 55g 38g 7g




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Green protein

White protein

Brown juice

22g 1g 14g

21g 1g 13g

25g 1g 17g

17g 1g 13g


Recovery Comparison 01-09-2015

Crude Protein

Green Juice

Green Protein

White Protein

Brown Juice

White clover

100g 57g 3g 40g





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Green Juice

pH 4 protein

pH 4 Brown juice

100g 70g 30g

100g 75g 25g

100g 65g 35g

100g 64g 36g

Recovered by Acidic Precipitation


Crude Protein Content 10 01-09-2015


0

5

10

15

20

25

30

35

40

45

50

Plant Pulp Green Juice pH 4 Green Protein White Protein Brown Juice

Cru

de

Pro

tein

[%

DM

]

Plant Fraction

Crude Protein

White clover

Red clover

Lucerne

Ryegrass

True Protein Content 11 01-09-2015


0

5

10

15

20

25

30

35

40

45

50

Whole plant Pulp Green Juice Green Protein White Protein Brown Juice

Tru

e P

rote

in [

%D

M]

Plant Fraction

True Protein

White clover

Red clover

Lucerne

Ryegrass

Limiting Amino Acids – Green Protein 12 01-09-2015


0

1

2

3

4

5

6

7

8

Met Met + Cys Lys His Thr Val

% P

rote

in

Amino Acid

Limiting Amino Acids - Green Protein

Soya bean [%CP]

White clover [%AA]

Red clover [%AA]

Lucerne [%AA]

Ryegrass [%AA]

Conclusion

• Locally grown plants contain high quality protein – Promising contents of extractable protein

– Satisfying amino acid composition

• Protein from legumes and grasses have a potential to replace a part of the imported soya bean meal

• Generally the legumes gave higher yield than the grass

• Examination of process induced modifications is needed

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Perspective

• Application of remaining fractions

– My ongoing project is examination of the value of the pulp for ruminant nutrition

• Optimisation of the process for purer products

• Higher efficiency of protein extraction and recovery

01-09-2015


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ANY QUESTIONS? Thank you for your attention E-mail: [email protected]

15 01-09-2015


Collaboration:

Yield and Amino Acid Composition of Pulp and Protein ... · Vinni Kragbæk Damborg, Lene...

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