Yeast Info

7/26/2019 Yeast Info

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Yeast Signal

Transduction LabSaccharomyces

cerevisiae


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Yeast are

Eukaryotes

Single celled organisms

Kingdom Fungi

Commonly used in biological experiments

Also used in commercial applications suchas bread and wine preparation


3/15

Fungi vs. Bacteria

FUN! "AC#E$!A

Eukaryote

Unicellular or%ulticellular

Cell &allNe'er photosynthetic

(rokaryote

Always Unicellular

Cell &all

Can be photosynthetic


4/15

Haploid Strains of Yeast

%A# a

)$elease *a +actor, pheromones

)-a'e receptors +or +actor

%A#

)$elease +actor pheromones

)-a'e receptors +or *a +actor,


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Yeast Mating

&hen the appropriate +actors are released. a

%A#and %A# a cell can mate

#he binding o+ the pheromone to the

receptor causes a signal transduction

pathway to be acti'ated/

Cells start to grow towards one another)

they shmoo0


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10/15

Diploid vs. Haploid

&hat are the ad'antages o+ being diploid1

)2 copies o+ e'ery gene

)Allows you to compensate +or a *bad, gene


11/15

Growt of Yeast

3E4 media)Complete media

)Any yeast should be able to grow on it/

%5 media)%inimal media

)6acks some nutrients that yeast might need)!+ a yeast cell can7t make these nutrients

themsel'es. they can7t grow on %5 media


12/15

Yeast !utants

Some yeast contain mutations that do not

allow them to produce speci+ic compounds

8 auxotrophic

&hen a yeast mutant is auxotrophic +or a

speci+ic compound. it will be unable to

grow on %5 media/


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Yeast !utants "continued#

!n order +or an auxotrophic yeast to grow

)#he compound has to be added to the media

9$

)#he yeast must mate with another yeast strain

that can complement it :so it can make the

compound; 8 complementation


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Lab $%peri!ent

&eek


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Your o!ewor&'

Study +or the (re 6ab >ui?

Set up lab notebook=

)&rite a S(EC!F!C title

)&rite an !ntro :2@ sentences;

)(aste in procedure

)%ake a +lowchart +or EAC- page o+ the

procedure

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