[XLS]xenopusresource.org · Web view736. 866. 766. 310. 344. 763. 785. 883. 857. 803. 918. 788....

Gene symbol Gene Name Gene synonyms Probe name Plasmid name Pictures Atribution Paper Vector Antibiotics Special Features

57-KL actc1 cardiac actin, actc cytoskeletal constituent pAC100 pAC100 no pictures p laevis pSP64 Amp Actin Cardiac

10-CC acvr1b RTK XALK4 XALK4 not needed p laevis pSP64T Amp Receptors

11-CC acvr1b RTK not needed p laevis pSP64T Amp Receptors

12-CC acvr2b RTK XActRIIB XActRIIB not needed p laevis pSP64T Amp Receptors

13-CC acvr2b RTK DN-XActRIIB DN-XActRIIB not needed p laevis pSP64T Amp Receptors

736 adcyap1 pacap signal transduction PACAP PACAP 6948249 have to upload them p laevis pCMV-SPORT6 Amp Image EST 6948249, full length cDNA

1-ER admp n/a TGFb pCS2 ADMP pCS2 ADMP no pictures laevis Int J Dev Bio 49,781-796 pCS2+ Amp Anti-dorsalizing morphogenetic protein full length

HAR-305 admp n/a n/a ADMP1/pcmv tropicalis pCMV amp Full length CF217288

HAR-290 n/a signalling protein n/a ADMP2 ADMP2 CS107 tropicalis CS107 amp Full length AL805193

HAR-78 ag1 anterior gradient 1 XAG, xag1, xag-1 secreted signalling XAG-2 TNeu029o22 trops 19 p tropicalis CS107 amp

HAR-99 ag1 anterior gradient 1 XAG, xag1, xag-1 signal modulator XAG IMAGE:5309001 n/a p tropicalis pCMV-SpPORT6.ccdb amp

3-ER ag1 XAG, xag1, xag-1, ag not known not in xenbase XAG-1 XAG-1/pGEM-T laevis pGEM-T-easy Amp Xenopus anterior gradient

866 ahctf1 elys neural TF ELYS IMAGE:6637561 have to upload them laevis pCMV-SPORT6.ccdb Amp Image EST 6637561, Accession BC076775

766 alas2 mgc89126 transferase ALAS2 IMAGE:6948792 yes p laevis pCMV-Sport6 Amp Image EST 6948792

310 aldh1a2 Retinoic acid synthesis n/a RALDH2 laevis

344 aldh1a2 retinoid metabolism pXRALDH2 p laevis Chen et al., 2001 PCS2+ AMP Full cDNA

336-NP als2 alsin n/a p tropicalis pBS SK(-) Amp

763 alyref n/a RNA binding Aly IMAGE:6952660 no pictures p laevis pCMV-Sport6 Amp Image EST 6952660, Ally of AML1

775-AP n/a cell cycle regulator anapc11 no pictures p pCS2+rep Amp PCR from IMAGE clone 7204223

785 angpt1 angiopoietin 1 ang-1, ang1 Ang1 IMAGE:5513391 no pictures p laevis pCMV-Sport6 Amp Image EST 5513391. Full length. Accession BC073367

883 angptl2 angiopoietin-like 2 Angptl2 Angptl2 (8076172) no pictures p laevis pExpress1 Amp Image EST 8076172. Accession BC124875

857 anpep n/a protease APN APN 4056527 no pictures p laevis pCMV-SPORT6 Amp Image EST 4056527, Accession BC085055

HAR-359 apaf1 apaf-1 n/a apaf1 apaf1 cs107 tropicalis CS107 amp not full length DT428857

803 apln apelin Apelin IMAGE:6631163 yes p laevis pCMV-Sport6 Amp Image EST 6631163

EXRC Number

Gene Function (for GMAG)

Xenbase Number

Sequence according to NCBI Blast March 2014

actin, alpha, cardiac muscle 1

XB-CLONE-12442439

activin A receptor, type IB

xalk4, activin type IB receptor, alk4, alk-4

XB-CLONE-12442180

activin A receptor, type IB

xalk4, activin type IB receptor, alk4, alk-4

XB-CLONE-12442183

Txalk4 (truncated ALK4)

Txalk4 - truncated ALK4

activin A receptor, type IIB

activin type IIB receptor, actriib, actr-

iibXB-CLONE-12442186

activin A receptor, type IIB

activin type IIB receptor, actriib, actr-

iibXB-CLONE-12442189

adenylate cyclase activating

polypeptide 1 (pituitary)

[Predicted: Source is Human Entrez

Gene]

XB-CLONE-2050896

anti-dorsalizing morphogenic

protein [provisional]

XB-CLONE-8713610

have to check when bug is solved

anti-dorsalizing morphogenic


tgf-beta family growth factor

admp2 [provisional]

antidorsalizing morphogenetic

protein 2 [provisional]

XB-CLONE-4330457

AL650560 - Tneu029o22*Probe works (has been tested)

XB-CLONE-4445458

BQ391796 - Full length. dbEST Id:12436621EST name: NISC_mq20g05.y1 - GenBank

Acc:BQ391796GenBank gi: 21079483

CLONE INFO: Full Length Clone Id: IMAGE:5309001 (5')

Source: IMAGE Consortium, LLNLCloned into Not I/RV site

anterior gradient 1

AT hook containing transcription factor

1Have to upload it, bug on page

aminolevulinate, delta-, synthase 2

XB-CLONE-2061754

aldehyde dehydrogenase 1

family, member A2 raldh2, XRaldh2, raldh-

2

aldehyde dehydrogenase 1

family, member A2 raldh2, XRaldh2, raldh-

2XB-CLONE-11536389

XB-IMG-48375 XB-IMG-48374

amyotrophic lateral sclerosis 2 (juvenile)

Junctional membrane complex protein Junctophilin and

related MORN repeat proteins

XB-CLONE-12442394

pbluescript sk(-) XL055h20

pbluescript sk- XL055h20

Aly/REF export factor [Predicted: Source is Human

Entrez Gene]

XB-CLONE-2073056

anapc11 [Predicted: Source is

Human Entrez Gene]

anaphase promoting complex

subunit 11 [Predicted: Source is Human Entrez

Gene]

XB-CLONE-12442091

anapc11-pCS2plus-repeired

laevis (17th down the list)

secreted endothelial growth factor

XB-CLONE-2046316

fbnl, fibrinogen-like protein, harp

vascular signalling molecule

XB-CLONE-1630514

alanyl (membrane)

aminopeptidase [Predicted: Source is Human Entrez

Gene]

XB-CLONE-2337624

apoptotic peptidase

activating factor 1

apoptosis/apoptosome scaffold protein with

WD-40 domains, caspase recruitment

domain (CARD), and an ATPase domain

preproapelin, Xapelin, apelin

secreted peptide precursor, angiogenic

XB-CLONE-2181121

use sequence 99b-Maya Piccinni


have to upload them-Maya Piccinni

try early stages if got time-Maya Piccinni

usto be XB-CLONE-12441870-Maya Piccinni

Trop sequence-Maya Piccinni


A1

use sequence 99b -Maya Piccinni

K12


I15

have to upload them -Maya Piccinni

A17

try early stages if got time -Maya Piccinni

F22

usto be XB-CLONE-12441870 -Maya Piccinni

K23

Trop sequence -Maya Piccinni

I25


HAR-177 aplnr apelin receptor TF msr TNeu054m15 p tropicalis CS107 amp NM_00103 - Full Length - Tneu054M15

1044-AP ash1, Xash1, Mash1 TF XAshI XAshI-pCS2plus not needed p laevis Curr Biol. July 13, 2000; 10 (14): 849-52. Amp

AP-125 neural TF not in xenbase XASH3 XASH3 laevis

HAR-250 atp1a1 ATPase n/a a1-ATPasea atp1a1 a1-ATPase tropicalis pCMV-SPO amp CF220660

918 atp1b1 Atp1b1a Atp1b1a (5570138) no pictures p laevis pCMV-SPORT6 Amp Image EST 5570138, Accession BC047957

HAR-195 bambi mgc89501 TGFb signal modulator BAMBI TEgg028m12 trops 21/28 p tropicalis CS107 amp AL881640 - Tegg028M12 - Full length

HAR-198 bambi mgc89501 TGFb signal modulator BAMBI TEgg127m13 n/a p tropicalis CS107 amp Accession number AL862749

NP-F basp1 n/a BASP1 TTpA072i19 no pictures tropicalis pCS107 Amp TEpA72i19

HAR-324 apoptosis regulator n/a bcl-Xl bcl-xl bclxl tropicalis pCMV-SPO amp Full length BC074505

HAR-443 bhlhe41 n/a not in xenbase sharp1 (dec2) dec2, bhlh tropicalis CS107 amp

4-ER bicc1 RNA binding Xbic-C Xbic-C pCS2plus no pictures p laevis pCS2+ Amp Xbicaudal-C

HAR-330 apoptosis inhibitor n/a Birc2 c-IAP1, BIrc2 tropicalis pExpress1 amp Full length CF378061

HAR-326 birc5.1 apoptosis inhibitor n/a Survivin survivin TIAP tropicalis CS107 amp Full length CR848111

HAR-327 birc5.1 apoptosis inhibitor n/a Survivin survivin TIAP birc5 tropicalis CS107 amp Full length CR848111

HAR-328 birc5.2 apoptosis inhibitor n/a SurvivinB survivinB tropicalis CS107 amp Full length CR761050

788 Bmi1a IMAGE:6956907 no pictures p laevis pCMV-Sport6 Amp Image EST 6956907. Full length. Accession BC082694

2-CC bmp2 BMP-2, xBMP-2, xbmp2 signalling molecule BMP2 BMP2 not needed p pSP64T Amp tgf-beta ligands

360 bmp2 BMP-2, xBMP-2, xbmp2 XBMP2-BR p laevis pBluescript KS+ Amp Includes approximately 2/3 of C-terminal domain

HAR-202 bmp2 BMP-2, xBMP-2, xbmp2 signalling protein BMP2 TEgg105i19 trops 11/28/19-30 p tropicalis CS107 amp Accession number AL864240

HAR-207 bmp2 BMP-2, xBMP-2, xbmp2 signalling molecule BMP2 TGas025a06 n/a p tropicalis CS107 amp

361 bmp4 XBMP4 p laevis pGEM11Zf(-) Amp

HAR-212 bmp4 signalling molecule BMP4 TNeu015d14 n/a p tropicalis CS107 amp Not Full length. Accession number AL636027.

HAR-193 bmp4 signalling protin n/a BMP4 bmp-4 bmp4 bone tropicalis CS107 amp not full length AL659162

apj, apjr, hg11, X-msr, msr, Xmsr, angio1,

Xangio1, agtrl1XB-CLONE-4254052

trops 22-28-2x unaidentifies

ascl1 [provisional]

achaete-scute complex homolog

1 [provisional]XB-CLONE-12442076

ascl3 [provisional]

achaete-scute family bHLH

transcription factor 3 [provisional]

hash4, bhlha44, sgn1, bhlha42, xash3

ATPase, Na+/K+ transporting, alpha

1 polypeptide

Na+/K+ ATPase, Na+K+ ATPase,

Na+K+ATPase, K-ATPase

ATPase, Na+/K+ transporting, beta

1 polypeptide

Na+-K+-ATPase, atp1b1a, Beta1-Na/K-

ATPaseion transporting

ATPase componentXB-CLONE-2193458

BMP and activin membrane-bound

inhibitorXB-CLONE-3968316

BMP and activin membrane-bound

inhibitorXB-CLONE-4100753

brain abundant, membrane

attached signal protein 1


Gene]

cap23, nap22, cap-23, nap-22

XB-CLONE-4201430

bcl2l1 [provisional]

BCL2-like 1 [provisional]

xR11, bcl-XL, bclxl, bcl-x(l)

basic helix-loop-helix family, member e41

basic helix-loop-helix (bHLH) transcription

factor

bicaudal C homolog 1

xBic-C, x-Bic-C, Bicaudal-C, Bicaudal C,

biccXB-CLONE-8718832

The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation.

Wessely O, De Robertis EM.Development. May 1, 2000; 127 (10): 2053-62.

birc2 [Predicted: Source is

Human Entrez Gene]

baculoviral IAP repeat containing 2 [Predicted: Source is Human Entrez

Gene]

api1, mihb, hiap2, rnf48, ciap1, hiap-2, c-

iap1

baculoviral IAP repeat-containing

5, gene 1

Survivin, xSurvivin1, svv1, Su2, BIR1, XBIR1,

api4, epr-1, xsurv

baculoviral IAP repeat-containing

5, gene 1

Survivin, xSurvivin1, svv1, Su2, BIR1, XBIR1,

api4, epr-1, xsurv

baculoviral IAP repeat containing 5

xSurvivin2, svv2, Su1, SIX

bmi1 [Predicted: Source is

Human Entrez Gene]

BMI1 polycomb ring finger oncogene


Gene]

xbmi-1, bmi-1, pcgf4, rnf51

Locus-specific chromosome binding

proteinsXB-CLONE-2051560

BMP-2, xBMP-2, xbmp2

XB-CLONE-12442159

Xenopus laevis bone morphogenetic protein

2 (bmp2-a)bone

morphogenetic protein 2

TGFb signalling molecule

XB-CLONE-12440878

XB-IMG-78488XB-IMG-78489XB-IMG-78490

bone morphogenetic

protein 2XB-CLONE-4098277

bone morphogenetic


Full Length - AL629718 - Tgas025A06Prepped by Alexandra Adler in February 2010

bone morphogenetic

protein 4

XBMP-4, bmp-4, xbmp4, bone

morphogenetic protein-4, zyme, bmp2b, ofc11,

bmp2b1


XB-CLONE-11536491

XB-IMG-78493 XB-IMG-78494 XB-IMG-78495


XB-IMG-78499

bone morphogenetic

protein 4



bmp2b1

XB-CLONE-4343367

bone morphogenetic

protein 4



bmp2b1



Trop sequence-Maya Piccinni



add as synonim of gene?-Maya Piccinni

F30


G40

add as synonim of gene? -Maya Piccinni

HAR-231 bmp4 signalling molecule n/a bmp4 bmp4 bmp-4 bone tropicalis CS107 amp AL961754

3-CC bmp4 signalling molecule BMP4 BMP4 not needed p laevis pSP64T Amp tgf-beta ligands

37 bmp4 n/a XBMP4 XBMP4/pSP64T laevis Dev. 115,573-585 1992 SP64T Amp

HAR-306 bmp7.1 bmp7, bmp-7, op-1 signalling molecule n/a BMP7 new BMP7 pCMV, tropicalis pCMVspor amp Full length BQ397135

HAR-310 bmp7.1 bmp7, bmp-7, op-1 signalling molecule n/a BMP7-CS110K BMP7 no pics tropicalis CS110K amp Full length - BQ397135

4-CC bmp7.1 bmp7, bmp-7, op-1 signalling molecule BMP7 BMP7 not needed p laevis pSP64T Amp tgf-beta ligands

HAR-61 bmp7.2 n/a ser/thr kinase receptor BMP7R IMAGE:4440598 trops 11 p tropicalis CS107 amp

14-ER bmper not in xenbase XCV2-FL XCV2-FL laevis Dev Cell 15,248-260 pCS2+ Amp Crossveinless-2 Full length

13-ER bmper not in xenbase XCV2 XCV2 laevis Dev Cell 15,248-260 pGEM Amp Crossveinless-2

445 bmpr1a ca-XBMPR not needed p laevis pSP64T amp

14-CC bmpr1a RTK tXALK3 tXALK3 not needed p laevis pSP64T Amp Receptors

534 bmpr2 BMP receptor BMPRII pCSXTR no pictures p laevis CS2+ Amp Full length XTALKr

5-ER xbtg-x anti-proliferation factor XBtg-X XBtg-x-pCS2plus no pictures p laevis pCS2+ Amp Xenopus B cell translocation gene-x

453-AP btrc GH releasing factor XlSlib pCS2plus Slib not needed p laevis pCS2+ Amp

HAR-246 n/a n/a C 3 tropicalis pCMV-SPO amp CF239870

HAR-393 n/a n/a calpain2 calpain tropicalis CS107 amp Full length CR761923 7822740

844 casp6 Cas6 IMAGE:4965757 p p laevis pCMV-SPORT6 Amp Image EST 4965757, Accession BC072796

354 cat2 cat2, atrc2 calcium transporter Xcat2 n/a p laevis Amp

790 cbfa2t2 MTGR1, XETOR neural TF MTGR1-like IMAGE:4680246 no pictures p laevis pCMV-Sport6 Amp Image EST 4680246. Full length. Accession BC057713

751 cbfa2t3 TF Eto2 IMAGE:3399101 no pictures p tropicalis pCMV-Sport6 Amp

bone morphogenetic

protein 4



bmp2b1

bone morphogenetic

protein 4



bmp2b1

XB-CLONE-12442162

bone morphogenetic

protein 4



bmp2b1

TGF-beta related peptide growth factor

bone morphogenetic

protein 7, gene 1 bone

morphogenetic protein 7, gene 1

bone morphogenetic

protein 7, gene 1XB-CLONE-12442165

bone morphogenetic

protein 7, gene 2XB-CLONE-4461855

BMP BMP-7 BMP7 bmp7 bmp-7 bmp-7R - Genbank ID#BG514944 - full length

bamH1 (t7) ok as well - NOT hind III*this clone has been tested and works for in situ

This clone encodes a protein more similar to "BMP-7R" of X. laevis than to "BMP-7," see Wang et al. 1997,

Genes and Function for comparison of proteins.see also clone 306 "BMP7-new"

BMP binding endothelial regulator

crossveinless-2, cv-2, CV2

BMP signalling modulator

BMP binding endothelial regulator

crossveinless-2, cv-2, CV2


bone morphogenetic

protein receptor, type 1A

alk3, alk-3, skr5, cd292, acvrlk3 serine/threonine kinase XB-CLONE-

12441596

bone morphogenetic

protein receptor, type 1A

alk3, alk-3, skr5, cd292, acvrlk3

XB-CLONE-12442192

bone morphogenetic

protein receptor, type 2

(serine/threonine kinase)

[provisional]

XBMPR-II, bmr2, pph1, bmpr3, brk-3, t-alk,

bmpr-ii, bmprii, BmprII XB-CLONE-12441225

btg5 [provisional]

B-cell translocation gene 5

[provisional]XB-CLONE-8756296

xBtg-x regulates Wnt/beta-Catenin signaling during early Xenopus development. Wessely O, Kim JI, Tran U,

Fuentealba L, De Robertis EM.Dev Biol. July 1, 2005; 283 (1): 17-28.

beta-transducin repeat containing

E3 ubiquitin protein ligase

Beta-TrCP, betaTrCP, fwd1, fbw1a, fbxw1,

btrcp, fbxw1a, btrcp1XB-CLONE-12442120

c3 [provisional]

complement component 3 [provisional]

activation of complement

Complement C3 edoderm

capns2 [Predicted: Source is

Human Entrez Gene]

calpain, small subunit 2


Gene]

Ca2+-binding protein, EF-Hand protein

superfamily

caspase 6, apoptosis-related

cysteine peptidasexCaspase-6, caspase-6,

caspase 6apoptotic cysteine

proteaseXB-CLONE-2207706

solute carrier family 7 (cationic

amino acid transporter, y+

system), member 2, gene 1


Gene]

XB-CLONE-12442622

core-binding factor, runt

domain, alpha subunit 2;

translocated to, 2

XB-CLONE-2249590



translocated to, 3

eto2, mtg16, mtgr2, zmynd4

XB-CLONE-2360176

Image EST 3399101. 99% identical to Image 5130002 at the nucleotide level




have to add as gene synonim-Maya Piccinni

Have map to upload

G64

have to add as gene synonim -Maya Piccinni

752 cbfa2t3 TF Eto2 IMAGE:5130002 no pictures p laevis pCMV-Sport6 Amp Image EST 5130002

588 cbfb CBFβ IMAGE:3404921 p laevis pCMV-SPORT6 Amp IMAGE EST 3404921

200-AP ccna1 cyclin A1 cyclin A1 cyclin XcycA1 cs2+ Xenopus Cyclin A1 laevis pCS2+ Amp Xenopus A1 cyclin148-AP ccna2 cyclin A2 n/a cyclin Xcyclin A2 Xcyclin A2 laevis pCS2+ MT Amp201-AP ccna2 cyclin A2 n/a cyclin Xcyclin A2 Xenopus Cyclin A2 laevis pCS2+ Amp146-AP ccnd1 cyclin D1 cyclin Xcyclin D1 Xcyclin D1 laevis pCS2+ MT Amp

147-AP ccnd2 cyclin D2 n/a cyclin Xcyclin D2 Xcyclin D2 no pictures p pCS2+ MT Amp

1089-AP n/a cell cycle regulator not in xenbase XLccndx Xlccndx laevis pCMVSPORT6 Amp

187-AP ccne, cyclinE G1/S-specific cyclin E XE1 XE1-CS2plus not needed laevis pCS2+ Amp Xenopus cyclin E1

762 cd93 cytoskeletal protein AA4 IMAGE:5515354 yes p laevis pCMV-Sport6 Amp Image EST 5515354

574-AP cdc20 X-FZY, fizzy1 typosine phosphatase Xcdc20 pCS2plus - Xcdc20 not needed p laevis pCS2+ Amp

JW-2379 cdc42 Xcdc42 small GTPase CS2-GFP wGBD eGFP sequence J Cell Biol. 2005 Jan 31;168(3):429-39. pCS2+ Amp GTPase activity

139-AP eg1 kinase not in xenbase Xcdk2 Xenopus cdk2 ? laevis pET8

879 xcdk4 Cdk4 IMAGE:5542266 no pictures p laevis pCMV-SPORT6 Amp Image EST 5542266. Accession BC051599

150-AP xcdk4 serine/threonine kinase Xcdk4 Xcdk4 pCS2plusMT no pictures p laevis pCS2+ MT Amp

AP-6 cdk5 n/a cyclin activator Xp35.2 FL Xp35-2 Fl Repaired no pictures p laevis pCS2+ Amp

AP-99 cdk5 Xcdk5 Xcdk5 pCS2plus not needed p laevis pCS2+ Amp

AP-44 cdk5r1 n/a cyclin activator Xp35.1 FL Xp35-1-FL no pictures p laevis pCS2+ Amp

AP-74 kinase inhibitor Xic1 FL Xic1 laevis pCS2+ Amp

6-ER cdx1 xcad2, xtcad2, cdx-1 TF not in xenbase Xcad2 Xcad2/pSK laevis Wessely O, Kim JI, Tran U, Fuentealba L, De Robertis EM. Amp Xenopus caudal 2 homeobox probe (full length clone)

HAR-314 cdx4 TF n/a cdx4 xcad3 xcad-3, cdx-4, tropicalis CS107 amp AL970478

JW-2493 Ca binding Xlt-Centrin2-RFP Nat Genet. 2008 Jul;40(7):871-9. pCS2+ Amp basal body/centrosome

8-ER cer1 not in xenbase cerberus-FL Cerberus-FL laevis Nature 382,595-601 pCS2+ Amp Cerberus Full length

9-ER cer1 not in xenbase xCer-S xCer-S laevis Nature 397,707-710 pCS2+ Amp Inhibition of the Nodal pathway Cerberus Short

7-ER cer1 cerberus pBS-cerberus laevis Dev Biol. July 1, 2005; 283 (1): 17-28. Amp Cerberus Insitu Probe

343 cer1 xcerS not needed p laevis pCS2

HAR-21 cer1 Cerberus L1G10f05 trops 17 p tropicalis CS107 amp Cereberus cereberus - Full Length - AL638969

HAR-36 cer1 Cerberus TGas016c17 n/a p tropicalis CS107 amp Tgas016C17 - Full Length - AL631153

7-CC cer1 wnt modulator Cerberus Cerberus not needed p laevis pCS105 Amp Extracellular Regulators

761 Ami, pfd, adipsin Trypsin Ami IMAGE:7204180 no pictures p laevis pExpress1 Amp Image EST 7204180



translocated to, 3


XB-CLONE-2224539

core-binding factor, beta

subunit

pebp2b, cbf-beta, pea2-beta, pebp2-beta,

cbfbetatranscription coactivator

XB-CLONE-2289271

have images, upload them when bug

resolved

prad1, parathyroid adenomatosis 1

XB-CLONE-12440817

Xenopus laevis cyclin D2

ccndx [provisional]

cyclin Dx [provisional]

ccne1 [Predicted: Source is

Human Entrez Gene]

cyclin E1 [Predicted: Source is Human Entrez

Gene]

XB-CLONE-12440825

CD93 molecule [Predicted: Source is Human Entrez

Gene]

c1qr1, c1qrp, cdw93, ecsm3, mxra4, c1qr(p),

c1qrXB-CLONE-2031832

cell division cycle 20

XB-CLONE-11536283

cell division cycle 42

wGBD fused to eGFP for visualizing active

Cdc42 in vivo

cdk2 [Predicted: Source is

Human Entrez Gene]

cyclin-dependent kinase 2


Gene]


Human Entrez Gene]



Gene]

serine / threonine kinase

XB-CLONE-2193068


Human Entrez Gene]



Gene]

XB-CLONE-12440822


XB-CLONE-12271744

Repaired by cutting at NcoI 5' and BsaBI 3' insert by PCR.



early-expressed neuronal cyclin-

dependent kinaseXB-CLONE-12271887

cyclin-dependent kinase 5,

regulatory subunit 1 (p35)

XB-CLONE-12271876

cdknx [provisional]

cyclin-dependent kinase inhibitor

xic1 [provisional] Xic-1, Xic1, p27XIC1,

p28caudal type

homeobox 1

caudal type homeobox 4

mgc68909, mgc130769, cad3,

Xcad3, Xtcad3, Xcad-3

Centrin-2 protein not in

xenbase?Centrin2Unigene ID:

Xl.50473laevis (centrin41st hit - centrin 2 lower down in

list) cerberus 1, DAN

family BMP antagonist

cerberus 1, DAN family BMP antagonist

wnt signalling modulator







XB-CLONE-8770484


xcer, xcer-1, xcer1, cer, Cerberus,

tCerberus, cer-1, dand4

secreted BMP antagonist

XB-CLONE-12440811

cerberus 1, DAN family BMP antagonis




XB-CLONE-4453188

cerberus 1, cysteine knot superfamily




XB-CLONE-4142498

cerberus 1, cysteine knot superfamily



XB-CLONE-12442171

cfd [Predicted: Source is

Human Entrez Gene]

complement factor D (adipsin)


Gene]

XB-CLONE-1820234




Have map to upload

add it as gene synonim?-Maya Piccinni


Maya Piccinni:Peter's p3-23


add as synonim-Maya Piccinni


B69

Have map to upload

G78

add it as gene synonim? -Maya Piccinni

I78


E79

Maya Piccinni: Peter's p3-23

F79


G85

add as synonim -Maya Piccinni

G86


HAR-1 chrd chordin chd, chordin, X-chordin Chordin TGas122k05 trops 11/13/28 p tropicalis CS107 amp

HAR-3 chrd chordin chd, chordin, X-chordin Chordin tropicalis CS107 amp Chordin chordin - 3' present - Tgas133K5

HAR-4 chrd chordin chd, chordin, X-chordin Chordin TGas083f21 no pics p tropicalis CS107 amp

HAR-6 chrd chordin chd, chordin, X-chordin Chordin TNeu011c22 no pics p tropicalis CS107 amp Chordin chordin - 5' present - Tneu011C22

HAR-7 chrd chordin chd, chordin, X-chordin Chordin TNeu140p12 no pics p tropicalis CS107 amp Chordin chordin - 5' present - AL797769 - Tneu140P12

HAR-13 chrd chordin chd, chordin, X-chordin Chordin TNeu029d07 no pics p tropicalis CS107 amp Chordin chordin - 3' present - AL646708 -

HAR-14 chrd chordin chd, chordin, X-chordin Chordin Tneu033C4 no pics p tropicalis CS107 amp Chordin chordin - 5' present - AL656923

HAR-16 chrd chordin chd, chordin, X-chordin Chordin TNeu005f12 no pics p tropicalis CS107 amp Chordin chordin - 3' present - AL635100

HAR-19 chrd chordin chd, chordin, X-chordin Chordin TNeu080l20 no pics p tropicalis CS107 amp Chordin chordin - not full length - AL784836

11-ER chrd chordin chd, chordin, X-chordin TGFb modulator not in xenbase Chordin-myc Chordin-myc laevis Cell 86,589-598 pCS2+ Amp Full length Chordin for injection

10-ER chrd chordin chd, chordin, X-chordin TGFb modulator Chordin Chordin no pictures p laevis Cell 79,779- Amp Chordin

6-CC chrd chordin chd, chordin, X-chordin TGFb modulator Chordin Chordin not needed p laevis PCS2+ Amp Extracellular Regulators

735-AP cks1b cks1, ckshs1 kinase regulator Xcks1 Xcks1 no pictures p laevis pCS2+ Amp

734-AP cks2 Xe-p9 kinase regulator Xcks2 Xcks2 laevis pCS2+ Amp

256-NP cldn4 claudin 4 Claudin 4 (13) Claudin 4 n/a p laevis pBS SK(-) Amp

LJ-11 cndp2 cn2, cpgl, darmin-r metalloprotease Darmin R pCS2 Darmin R pCS2 not needed p laevis pCS2+ Amp

737 cntn1 contactin 1 cell adhesion Contactin A Contactin 4743353 one picture to upload p laevis pCMV-Sport6 Amp Image EST 4743353

HAR-288 col11a3, xcol2a1 structural protein n/a col2a1 tropicalis pCMV-Spor amp BC063191

HAR-55 coro1c hcrnn4 actin binding Coronin IMAGE:4440788 n/a p tropicalis CS107 amp

768 n/a Zinc carboxypeptidase CPO IMAGE:6860923 no pictures p tropicalis pCMV-Sport6 Amp Image EST 6860923, Heme biosynthesis enzyme

HAR-283 crx TF n/a Otx5b Otx5b tropicalis CS107 amp CR848167

HAR-242 Chito chitinase n/a Chito tropicalis pExpress amp CF375459

HAR-394 ctgf XCTGF growth factor CTGF IMAGE:7020823 no pics p tropicalis pExpress-1 amp Full Length - 7803217

889 ctnnal1 cllp, alpha-catu Catnal1 IMAGE:3402292 not in xenbase p laevis pCMV-SPORT6 Amp Image EST 3402292. Accession BC077179

HAR-236 ctnnb1 beta-catenin n/a beta-catenin beta-catenin b-catenin tropicalis CS107 amp CR761449

20-SPH ctnnb1 beta-catenin Xenopus Beta-catenin pCS2+ pt Xβ-catenin not needed p laevis Yost et al. Genes and Development pCS2+ AMP

54-SPH ctnnb1 beta-catenin not in xenbase pXexβcatmyc pXexβcatmyc laevis unpublished pXex AMP Jeff Miller, University of Minnesota

847 connexon component Cx38 Cx38 6319277 have to upload them p laevis pCMV-SPORT6 Amp Image EST 6319277, Accession BC068650

558 cxcr4 CXCR4 xCXCR4 p laevis Moepps et al. 2000. Eur J Immunol. 30: 2924-2934 pBluescript SK- Amp


XB-CLONE-4035603

Chordin chordin - not full length - AL963268 - Tgas122K5


not in xenbase - no accession

number


XB-CLONE-4116469

Chordin chordin - not full length - AL774744 - Tgas083F21


XB-CLONE-4338641


XB-CLONE-4281195


XB-CLONE-4347147


XB-CLONE-14574917


XB-CLONE-4344809


XB-CLONE-4241771

XB-CLONE-8844969

XB-CLONE-14575125

CDC28 protein kinase regulatory

subunit 1BXB-CLONE-12442095

CDC28 protein kinase regulatory

subunit 2

cpe-r, cper, cpetr, cpetr1, wbscr8

integral membrane protein, tight junction

componentXB-CLONE-12442383

CNDP dipeptidase 2

(metallopeptidase M20 family)

XB-CLONE-11536603

F3/F11, contactin/F3/F11

XB-CLONE-2267622

col2a1 [Predicted: Source is

Human Entrez Gene]

collagen, type II, alpha 1 [Predicted: Source is Human

Entrez Gene]

Collagen II A, Collagen A

coronin, actin binding protein, 1C

XB-CLONE-4461801

coronin - 4440788 - not full length - Genbank ID# BG512194

dad26g11.y1 Wellcome CRC pCS107 tropicalis St10-12 Silurana tropicalis cDNA clone IMAGE:4440788 5' similar to TR:Q9PWG8 Q9PWG8 CORONIN HOMOLOG. ;, MRNA

sequence

cpo [Predicted: Source is

Human Entrez Gene]

carboxypeptidase O [Predicted:

Source is Human Entrez Gene]

XB-CLONE-2158685

cone-rod homeobox

otx5, Xotx5, Xotx5b, otx5b

ctbs [Predicted: Source is

Human Entrez Gene]

chitobiase, di-N-acetyl- [Predicted: Source is Human

Entrez Gene]

Chito endoderm Glycosyl

connective tissue growth factor

XB-CLONE-4362656

catenin (cadherin-associated

protein), alpha-like 1 [Predicted:


membrane cytoskeleton

XB-CLONE-2317939


protein), beta 1, 88kDa

cell adhesion/wnt signalling




XB-CLONE-14573953

Xenopus beta-catenin with point mutations in some of the N-terminal phosphorylation sites, which makes this beta-catenin refractory to degredation (i.e. more stable

protein, stronger activity)




have to add them when

bug is resolved

Cx38 gene not available in xenbase

XB-CLONE-2185417

chemokine (C-X-C motif) receptor 4

xcxcr4, fb22, hm89, lap3, lcr1, npyr, cd184,

lestr, npy3r, npyrl, hsy3rr, npyy3r

G-protein coupled receptor

XB-CLONE-11536494

XB-IMG-48395XB-IMG-48396XB-IMG-48397XB-IMG-48398XB-IMG-48132XB-IMG-48131








add as synonim?-Maya Piccinni

G111

add as synonim? -Maya Piccinni

472 cyp26a1 RA degradation CYP26 XCYP26 p laevis pGEM3Zf(-) Amp PCR from stage 12 RA treated embryos

764 xdab2, doc2, doc-2 adaptor protein DAB2 IMAGE:4030688 no pictures p laevis pCMV-Sport6 Amp Image EST 4030688

765 xdab2, doc2, doc-2 adaptor protein XDab2-pCS2 not needed p laevis Cheong et al 2006. BMC Dev Biol 6: 63 pCS2+ Amp

HAR-244 darmin darmin metalloprotease n/a Endocut endocut Glutamate tropicalis pCMV-SPO amp CF222137

HAR-96 dazap2 n/a RNA binding Dazap2 TNeu027k13 trops 17/23/41 tropicalis CS107 amp AL644285

97-KL dazap2 n/a Proline-rich protein 81C8A-81E10C no pictures p laevis pCS107 Amp

353 dazl RNA binding XDazl n/a p laevis PCS2+ Amp

915 ddit3 gadd153, Xchop, chop CHOP CHOP (7009631) XB-IMG-78859 p laevis pCMV-SPORT6 Amp

355 ddx25 deadsouth, grth, xcat3 RNA helicase Xcat3 p laevis Amp

747 dhh desert hedgehog signalling molecule Xchh Xchh-pBluescript SK - no pictures p p pBluescript SK (-) Amp cDNA X30

299 dkk1 pCS2plus DKK1 not needed p laevis PCS2+

300 dkk1 pRNdkk-1 p laevis pRN amp globin3' UTR deleted in pRN

HAR-2 dkk1 dkk1 TGas131c10 trops 13/18/28/ 33/34 p tropicalis CS107 amp

115-KL dll1 delta-like 1 Notch signal modulator X-Delta-1-STU X-Delta-1-STU not needed p laevis pCS2 Amp Dominant active Delta-1

327-AP dll1 delta-like 1 notch receptor X-Delta-1 X Delta-1 pCS2plus no pictures p laevis pCS2+ Amp X-Delta-1

442-AP dll1 delta-like 1 not in xenbase XDStn Delta Stn N4 laevis pCS2+ Amp

891 dll4 delta-like 4 delta-like 4 Dll4 Dll4 (7876232) not in xenbase p tropicalis pCS107 Amp Image EST 7876232

HAR-307 dlx2 X-dll2, tes1, tes-1 TF n/a dlx2 dlx-2, distal-less tropicalis pCMV-SPO amp Full length BC080947

HAR-120 xdlx3, ai4, tdo TF dlx3 TGas040e17 trops 19/24/27/28/32 p tropicalis CS107 amp

HAR-450 xdlx3, ai4, tdo Dlx3 IMAGE:5308608 no pictures p tropicalis pCMV-SPORT6.1 amp

HAR-116 dlx5 dll3, X-dll3 TF dlx5 TNeu139d17 Trops 14/15/26/28 p tropicalis CS107 amp

HAR-381 dlx5 dll3, X-dll3 n/a Dlx5 (DLL3) tropicalis CS107 amp

40-NP dlx5 dll3, X-dll3 X-Dll 3 X-Dll 3 no pictures p laevis PKS Amp

HAR-362 dnd1 n/a DeadEnd Dead end tropicalis CS107 amp not full length BX771604

cytochrome P450, family 26,

subfamily A, polypeptide 1

xCYP26, XCYP26A1, cyp26, RACE, retinoic

acid converting enzyme

XB-CLONE-11536453


dab2 [Predicted: Source is

Human Entrez Gene]

disabled homolog 2, mitogen-responsive

phosphoprotein [Predicted: Source is Human Entrez

Gene]

XB-CLONE-2111330

dab2 [Predicted: Source is

Human Entrez Gene]

disabled homolog 2, mitogen-responsive

phosphoprotein [Predicted: Source is Human Entrez

Gene]

XB-CLONE-8596678

endocut, glutamate carboxypeptidase-like

protein 1DAZ associated


DAZ associated protein 2

transcription coactivator

XB-CLONE-2301880

Use of large-scale expression cloning screens in the Xenopus laevis tadpole to identify gene function.Grammer TC, Liu KJ, Mariani FV, Harland RM.Dev Biol. December 15, 2000; 228 (2): 197-210.

1kb from st19-23 library. Caused anterior defects and krox20 loss with ectopic staining. GeneBank BG354661.

deleted in azoospermia-like


Gene]

Xdazl, dazh, spgyla, dazla, dazl1

XB-CLONE-12440875

A Xenopus DAZ-like gene encodes an RNA component of germ plasm and is a functional homologue of Drosophila

boule.Houston DW, Zhang J, Maines JZ, Wasserman SA, King ML.

Development. January 1, 1998; 125 (2): 171-80.

DNA-damage-inducible transcript

3

basic-leucine zipper (bZIP) transcription

factorXB-CLONE-2024956

Image EST 7009631, Accession BC157447. Also known as Gadd153

DEAD (Asp-Glu-Ala-Asp) box helicase 25


Gene]

XB-CLONE-12442625

XB-IMG-48640XB-IMG-48611

cephalic hedgehog, chh, x-chh, dhh-1,

desert hedgehog, XchhXB-CLONE-12441517

Distinct expression and shared activities of members of the hedgehog gene family of Xenopus laevis.Ekker SC, McGrew LL, Lai CJ, Lee JJ, von Kessler DP, Moon RT, Beachy PA.Development. August 1, 1995; 121 (8): 2337-47.

dickkopf WNT signaling pathway

inhibitor 1

dickkopf-1, Xdkk-1, dkk-1, dkk, xdkk1,

Dickkopf1 secreted wnt antagonist

XB-CLONE-12271686


inhibitor 1


Dickkopf1 secreted wnt antagonist

XB-CLONE-12442782



inhibitor 1


Dickkopf1wnt signalling

modulatorXB-CLONE-4006403

Dickkopf-1 dickkopf1 - Full length - AL969277 - TGas131c10

X-delta-1, delta1, delta-1, Xdelta-1,

XDelta1, x-delta, Delta-1, Xdelta1

XB-CLONE-12442504



XB-CLONE-12440849



notch signalling modulator


XB-CLONE-3105910

distal-less homeobox 2

dlx3 [provisional]

distal-less homeobox 3 [provisional]

XB-CLONE-4122930

AL653760 - Uncertain - Likely have 3'Don't have 5'TGAS040e17

dlx3 [provisional]


homeodomain and LIM zinc binding domain transcription factor

XB-CLONE-4446818


XB-CLONE-4283302

AL796264 - 3' present - don't have 5'TNeu139d17

EcoRI-NcoI - 300 bp band





XB-CLONE-12442258

dead end homolog 1

dead-end, dead end, xde, Xdead, dnd

nuclear ribonucleoprotein R (RRM superfamily)









Unaidentifed vector-Maya Piccinni

pRN Vector?-Maya Piccinni

F136

Unaidentifed vector -Maya Piccinni

A139

pRN Vector? -Maya Piccinni

1095-AP metabolic enzyme XCRMp4 XCRMp4 laevis pCMVSport6 Amp

166-KL dvl1 XdshWT XdshWT not needed p laevis pCS2+ Amp GFP disheveled. Xdsh 2190 Bfal Fragment

167-KL dvl1 XdshD1 XdshD1 not needed p laevis pCS2+ Amp

407 dvl2 pCS2-myc-Dsh-DC not needed p laevis Development 127, 2227-2238(2000) pCS2-myc amp

408 dvl2 Xdd1 (Dsh-DPDZ) not needed p laevis pCS2-myc amp

411 dvl2 PCS2-myc-Dsh-DN not needed p laevis Development 127, 2227-2238 (2000) pCS2-myc

412 dvl2 pCS2-myc-Dsh-DEPplus not needed p laevis Development 127, 2227-2238 (2000) pCS2-myc

168-KL dvl2 XdshD2 XdshD2 not needed p laevis pCS2+ Amp

JW-1203 dvl2 n/a XdshD2 laevis Rothbächer U, et al.EMBO J. 2000 Mar 1;19(5):1010-22. pCS2+ Amp

JW-1206 dvl2 n/a XdshD6 laevis Rothbächer U, et al.EMBO J. 2000 Mar 1;19(5):1010-22. pCS2+ Amp

JW-1201 dvl2 n/a XdshWT laevis Rothbächer U, et al.EMBO J. 2000 Mar 1;19(5):1010-22. pCS2+ Amp

141-AP e2f1 xE2F cell cycle TF XE2F Xenopus E2F laevis pGEM 2F

52-NP TF X Coe 2 XCoe2 no pictures p laevis Amp

175-NP Xebf3, coe3 TF CS2+ XEBF3 CS2plus XEBF3 n/a p laevis CS2+ Amp

174-NP Xebf3, coe3, oe2 TF XEBF3 Full Coding XEBF3 Full Coding no pictures p laevis pBlueScript II Sk+ Amp

HAR-103 eef1a1 translation initiation EF1alpha EF1alpha tropicalis CS108 amp

HAR-179 efnb2 ephrin-B2 ephrinB2, ephrin B2 signalling molecule efnb2 ephrinB2 ephrin-B2 tropicalis pCMV-sport6.1 amp

13-KL efnb2 ephrin-B2 ephrinB2, ephrin B2 signalling molecule ephrin-B2 ephrin-B2 no pictures p laevis pBSK Amp Ephrin

HAR-237 efnb2 ephrin-B2 ephrinB2, ephrin B2 signalling molecule n/a efnb2 ephrin b2 tropicalis pCMV-SPO amp BQ390329786 efnb2-a ephrin-B2 ephrinB2, ephrin B2 signalling molecule EphrinB2 IMAGE:4724740 no pictures p laevis pCMV-Sport6 Amp Image EST 4724740. Full length. Accession BC057724

6 egr2 transcription factor (TF) XKROX20 XKROX20 no p laevis pBluescript II KS+ Amp shorter version is poor, do not use.

58-KL egr2 Bs.EP400 Bs EP400 no pictures p laevis pBKS- Amp Krox20

309 egr2 n/a XKrox20 tropicalis

1093-AP elavl3 elrC RNA binding Elrc Elrc laevis

dpysl3 [Predicted: Source is

Human Entrez Gene]

dihydropyrimidinase-like 3 [Predicted: Source is Human

Entrez Gene]

CRMP-4, drp3, ulip, crmp4, drp-3, lcrmp, ulip-1, tuc4, tuc-4,

nsp1

dishevelled, dsh homolog 1

dsh1, dishevelled1, Xdsh


XB-CLONE-12442531


dsh1, dishevelled1, Xdsh


XB-CLONE-12442533

Disheveled GFP DIX deletion. Deleted DIX and flank 2190 BfaI fragment.


Xdsh, dsh, dishevelled, dvl


XB-CLONE-12440933




XB-CLONE-12441151

Sokol SY: Analysis of Dishevelled signalling pathways during

Xenopus development. Curr Biol 1996, 6:1456–1467.

dishevelled segment polarity

protein 2 Xdsh, dsh, dishevelled,

dvl wnt signalling



protein 2 Xdsh, dsh, dishevelled,

dvl wnt signalling





XB-CLONE-12442535

Disheveled GFP PDZ deletion. 2190 BfaI fragment deleted 707-990.


protein 2Xdsh, dsh, dishevelled,

dvl wnt signalling

modulatorXdsh2190 BfaI

fragment delete 707-990

PDZ mutant Dvl2. For expression of disheveled mRNAPDZ deletionaxis-inducing membrane

translocating phosphorylatedCHECK ORIGINAL DETAILED INFO IN PLASMID

BINDER



dvl wnt signalling


fragment

DEP mutant Dvl2. For expression of disheveled mRNAMissing most of DEP and C-terminal GFP

taggedaxis-inducing YES membrane translocating NO phosphorylated

NOCHECK ORIGINAL DETAILED INFO IN PLASMID BINDER



dvl wnt signalling


fragment

WT Dvl2. For expression of disheveled mRNAamplified 5'TGCCGTGCTTTAATGG/

ACCG5'GGTCCCAGCATTTGGCCAC fragment used to screen

cDNA librariesHA tag near N-terminusaxis-inducing membrane translocating

phosphorylatedCHECK ORIGINAL DETAILED INFO IN PLASMID BINDER

E2F transcription factor 1

ebf2 [Predicted: Source is

Human Entrez Gene]

early B-cell factor 2 [Predicted: Source is Human Entrez

Gene]

coe2, xcoe2, Xebf2, Mmot1, oe3

XB-CLONE-14575113


Human Entrez Gene]


Gene]

XB-CLONE-12442362


Human Entrez Gene]


Gene]

XB-CLONE-12442359

eukaryotic translation

elongation factor 1 alpha 1

EF-1 alpha, eef1a-s, elongation factor-1

alpha, EF1alpha

not in xenbase - no accesion

number

Don not know how to put it,

efnb2 or ephb1

BQ388674-accession number given, but that clone is under the gene ephb1. NISC_mq03c02.y1

NICHD_XGC_Emb5 Silurana tropicalis cDNA clone IMAGE:5307266 5', MRNA sequence

gi|21076361|gb|BQ388674.1|[21076361]

NISC_mq03c02.x1 NICHD_XGC_Emb5 Silurana tropicalis cDNA clone IMAGE:5307266 3', MRNA sequence

gi|21076360|gb|BQ388673.1|[21076360]

XB-CLONE-12442410

The EphA4 and EphB1 receptor tyrosine kinase and ephrin-B2 ligand regulated targeted migration of branchial neural crest cells. Smith, Robinson, Patel & Wilkinson, Curr Biol 1997 Aug 1;7(8):561-70

XB-CLONE-2203940

early growth response 2 (Krox-

20 homolog) XKr20, EGR-2, Krox-20, XKrox-20, krox20

XB-CLONE-11536560


20 homolog)XKr20, EGR-2, Krox-20,

XKrox-20, krox20zinc finger transcription



20 homolog) XKr20, EGR-2, Krox-20,

XKrox-20, krox20zinc finger transcription

factor

ELAV like neuron-specific RNA

binding protein 3









Unaidentifed vector-Maya Piccinni

pRN Vector?-Maya Piccinni

Check vector-Maya Piccinni

not in xenbase-Maya Piccinni




This clone seems to be repeated. same name than number 6. No info on this number. Have stocks of both.

-Maya Piccinni

F155

Check vector -Maya Piccinni

M155

not in xenbase -Maya Piccinni

M156


M157


M158


A174

This clone seems to be repeated. same name than number 6. No info on this number. Have stocks of both. -Maya Piccinni

323 elf1 Elf-1, xelf1 neural TF x-elf1 1 n/a p laevis pCS107 Amp

324 elf1 Elf-1, xelf1 neural TF x-elf1 2 p laevis pCMV SPORT6 Amp

325 elf1 Elf-1, xelf1 Zn ribbon containing x-elf1 3 p laevis pCMV SPORT6 Amp

838 elk3 erp, sap2 dorsal TF Elk3 IMAGE:4963943 have to upload them p laevis pCMV-SPORT6 Amp Image EST 4963943. Accession BC056051

839 elk4 sap1 head, kidney, somite TF Elk4 IMAGE:7011856 have to upload them p laevis pCMV-SPORT6 Amp Image EST 7011856, Accession BC074199

876 foap-10, emilin-2 Emilin2 IMAGE:8072697 yes p laevis pExpress1 Amp Image EST 8072697. Accession BC127409

HAR-251 XEmx1 TF n/a Emx1 emx1 empty spiracles 1 tropicalis pCMV-SPO amp BQ520008

HAR-255 emx2 xemx2, emx-2 TF n/a emx2 emx-2 emx2 tropicalis CS107 amp CR407807

98-NP en2 TF EN 2 (p33) EN2p33 no pictures p laevis pBluescript II KS Amp

LJ-75 n/a phosphodiesterase enpp4 pCDNA enpp4 pCDNA laevis

LJ-81 enpp6 n/a phosphodiesterase enpp6 pBF enpp6 pBF laevis

LJ-67 n/a not known not in xenbase enbpd6 enbpd6 laevis pcDNA3

336 eomes eomesodermin mesoderm TF pEomes RN3-3 not needed p laevis Cell, Vol. 87 989-1000 RN3 AMP

553 eomes eomesodermin Mesoderm TF Eomesodermin pEomes/Bsc laevis Ryan et al. 1996. Cell 87: 989-1000 pBluescript Amp

HAR-205 eomes eomesodermin TF Eomes TGas015d23 no pics p tropicalis CS107 amp

HAR-204 eomes , scaf1 eomesodermin TF Eomes TGas141f06 p tropicalis CS107 amp Full Length - AL957959

HAR-34 epha4 RTK epha4 (Sek1/ EphA4) TNeu011c01 trops 21/28 p tropicalis CS107 amp

14-KL RTK EphA4 truncated EphA4 truncated no pictures p laevis SP64TK Amp

106-KL RTK EphA4 EphA4 no pictures p laevis pBSK Amp 2.6kb EphA4

HAR-184 ephb4 EPH receptor B4 htk, myk1, tyro11 signalling molecule ephb4 TGas046k17 p laevis sequence CS107 amp not full length AL651958

HAR-185 ephb4 EPH receptor B4 htk, myk1, tyro11 signalling molecule ephb4 TNeu111k19 n/a p laevis sequence CS107 amp not full length AL793588

65-NP etv1 ets variant 1 XER81, er81 TF XER 81 XER 81 no pictures p laevis pBKCMV Kan66-NP etv1 ets variant 1 XER81, er81 TF XER 81 XER 81 no pictures laevis ? AMP

835 er71, etsrp71 TF Etsrp Etsrp-T7TS not needed p laevis From Paul Krieg T7TS Amp Entire ORF lifted by PCR from EST BC099054

792 etv6 ets variant 6 TF Tel1b Tel1b-Sport6 no images p laevis pCMV-Sport6 Amp

E74-like factor 1 (ets domain transcription

factor)

XB-CLONE-12271649


factor)

XB-CLONE-12271654



factor)

XB-CLONE-12271660

XB-IMG-48648; XB-IMG-48601

ELK3, ETS-domain protein (SRF

accessory protein 2)

XB-CLONE-2253705

ELK4, ETS-domain protein (SRF

accessory protein 1)

XB-CLONE-2025384

emilin2 [Predicted: Source is

Human Entrez Gene]

elastin microfibril interfacer 2


Gene]

Uncharacterized conserved coiled-coil

proteinXB-CLONE-1620850

emx1 [provisional]

empty spiracles homeobox 1 [provisional]

empty spiracles homeobox 2

engrailed homeobox 2

engrailed homolog 2, En-2, engrailed-2, engrailed 2, eng2,

xen2, maben

XB-CLONE-12442341

enpp4 [Predicted: Source is

Human Entrez Gene]

ectonucleotide pyrophosphatase/phosphodiesterase 4

(putative) [Predicted: Source is Human Entrez

Gene]

ectonucleotide pyrophosphatase/phosphodiesterase 6 [Predicted: Source is Human Entrez

Gene]

entpd6 [Predicted: Source is

Human Entrez Gene]

ectonucleoside triphosphate

diphosphohydrolase 6 (putative)


Gene] xeomes,

eomesodermin XB-CLONE-12271667

xeomes, eomesodermin

XB-CLONE-12441236


could not find the

paper online


XB-CLONE-4142366

AL631245 - Tgas015D233' end gives no hits


XB-CLONE-3992797

trops 12, 13, 16, 21, 25, 28, 40

EPH receptor A4 [Predicted: Source is Human Entrez

Gene]

sek, hek8, tyr01, rtk1, XEpha4, sek-1

XB-CLONE-4348215

sek1 sek SEK Sek1 Sek EphA4 eph - Uncertain - Tneu011C01 - AL638286

*this clone has been tested and works for in situ

epha4 [Predicted: Source is

Human Entrez Gene]


Gene]


XB-CLONE-12442413

The EphA4 and EphB1 receptor tyrosine kinase and ephrin-B2 ligand regulated targeted migration of branchial neural crest cells. Smith, Robinson, Patel & Wilkinson, Curr Biol 1997 Aug 1;7(8):561-70

Truncated EphA4 encoding extracellular and transmembrane domains cloned into SP64TK

epha4 [Predicted: Source is

Human Entrez Gene]


Gene]


XB-CLONE-12442498

The EphA4 and EphB1 receptor tyrosine kinases and ephrin-B2 ligand regulate targeted migration of branchial

neural crest cells.Smith A, Robinson V, Patel K, Wilkinson DG.

Curr Biol. August 1, 1997; 7 (8): 561-70.

XB-CLONE-4125768

Trops 14-20-28-unspecified stage

XB-CLONE-4241394

XB-CLONE-14575119

etv2

[Predicted: Source is

Human Entrez Gene]

ets variant 2 [Predicted: Source is Human Entrez

Gene]

XB-CLONE-14586091

mgc146892, TEL oncogene

XB-CLONE-12441582

Image EST 6929431 subcloned in to pCMV-Sport6 NOTE: Name as Tel1 gene but sequence and accession

number refers to etv6 gene.















-Maya Piccinni


Vector not in xenbase-Maya Piccinni

Add as sinonym-Maya Piccinni

I181


F188

Vector not in xenbase -Maya Piccinni

G199

Add as sinonym -Maya Piccinni

237-SPH evx1 Xhox3, eve1 TF not in xenbase pBS-Xhox3s2 pBS-Xhox3s2 no pictures laevis Isaacs et al 1994 pBS AMP Xhox3

HAR-416 fabp2 IFABP, fabp2-A IFABP IMAGE:7833732 no pics p tropicalis CS107 amp 7833732

481-AP emi1, fbx5 cytoskeletal XEMI1 pCS2 laevis pCS2 Amp

607-AP cdc4 typosine phosphatase Xcdc4 Xcdc4-pBSKplus not needed p laevis pBSK+ Amp

606-AP cdc4 typosine phosphatase Xcdc4 Xcdc4-pCS2plus-rep n/a p laevis pCS2+rep Amp

HAR-415 fga Fibrinogen IMAGE:7786024 no pics p tropicalis CS108 amp 7786024, LLAM 16997 g 14

HAR-404 fgf1 Fgf1 tropicalis pGEM-T amp Not full length

HAR-400 fgf10 fgf-10 Fgf10 TNeu116h04 no pics p tropicalis CS107 amp

HAR-407 fgf12 n/a Fgf12 tropicalis pGEM-T amp Full Length

HAR-408 fgf13 mgc81084 Fgf13 tropicalis pGEM-T amp Full Length

HAR-409 fgf14 fhf4, sca27 Fgf14 tropicalis pGEM-T amp Full Length

HAR-410 fgf16 n/a Fgf16 tropicalis pGEM-T amp Full Length

HAR-396 fgf2 fgf2 Tegg044c22 no pics p tropicalis CS107 amp Full length - Tegg044c22 - Accession CR848523

HAR-411 fgf20 XFGF-20, xfgf20 Fgf20 tropicalis pGEM-T amp Not full length

HAR-398 fgf3 fgf-3, xfgf3 n/a pBSK:Fgf3 fgf3 tropicalis CS107 amp

HAR-405 fgf4 Fgf4 tropicalis pGEM-T amp Full Length

70-NP fgf4 RTK XFGFR - 4a XFGFR-4a no pictures p laevis Bluescript II SK Amp

26 fgf4 n/a eFGF pSPHB+ laevis SP64T Amp

27 fgf4 n/a a/s eFGF pSPHB- laevis pSP64T Amp

HAR-399 fgf7 kgf Fgf7 TNeu035m16 no pics p tropicalis CS107 amp Tneu035m16

HAR-90 fgf8 signalling molecule FGF8 TNeu007i10 trops 10 p tropicalis CS107 amp AL642603 - Tneu007i10

HAR-467 fgf8 xtFGF8acmvspt6 xtFGF8a-cmvspt6 no pictures p tropicalis pCMVsport6 amp

HAR-468 fgf8 xtFGF8bcmvsprt6 xtFGF8b-cmvsprt6 no pictures p tropicalis pCMVsport6 amp

HAR-469 fgf8 xtFGF8acds108 xtFGF8a-cds108 not needed p tropicalis pCS108 amp

HAR-470 fgf8 xtFGF8bcds108 xtFGF8b-cds108 not needed p tropicalis pCS108 amp

even-skipped homeobox 1


bug is resolved

fatty acid binding protein 2, intestinal

fatty acid uptake, transport, and metabolism

XB-CLONE-3368984

fbxo5 [Predicted: Source is

Human Entrez Gene]

F-box protein 5 [Predicted: Source is Human Entrez

Gene]

fbxw7 [Predicted: Source is

Human Entrez Gene]

F-box and WD repeat domain

containing 7, E3 ubiquitin protein ligase [Predicted: Source is Human

Entrez Gene]

XB-CLONE-11536290

fbxw7 [Predicted: Source is

Human Entrez Gene]

F-box and WD repeat domain

containing 7, E3 ubiquitin protein ligase [Predicted: Source is Human

Entrez Gene]

fibrinogen alpha chain

Fibrinogen, A alpha polypeptide, fibrinogen

blood-clotting protein component

XB-CLONE-2941370

fibroblast growth factor 1 (acidic)

HBGF-1, aFGF, acidic fgf, fgf-1

heparin-binding growth factor

no accession number

fibroblast growth factor 10


XB-CLONE-4238815



no accession number



no accession number



no accession number



no accession number

fibroblast growth factor 2 (basic)

HBGF-2, bfgf, fgf-2, basic fgf, xfgf2


XB-CLONE-3991729



no accession number



fibroblast growth factor 4 (heparin

secretory transforming

protein 1, Kaposi sarcoma

oncogene)

efgf, HBGF-4, XEFGF, fgfe, fgf-4, Xfgf4, Xfgf-4


no accession number




oncogene)

efgf, HBGF-4, XEFGF, fgfe, fgf-4, Xfgf4, Xfgf-4

XB-CLONE-14575122




oncogene)

efgf, HBGF-4, XEFGF, fgfe, fgf-4, Xfgf4, Xfgf-

4, fgf4-b heparin-binding

growth factor




oncogene)

efgf, HBGF-4, XEFGF, fgfe, fgf-4, Xfgf4, Xfgf-

4, fgf4-b heparin-binding

growth factor



XB-CLONE-4327133

fibroblast growth factor 8 (androgen-

induced)

mgc79739, Xfgf-8, Xfgf8, FGF-8, fgf8a,

fgf8b, fgf-8bXB-CLONE-4334291


induced)


fgf8b, fgf-8bheparin-binding growth



induced)





induced)





induced)
















-Maya Piccinni



Maya Piccinni:ex p3-32


E205

Maya Piccinni: ex p3-32

F205

Maya Piccinni: ex p3-32

HAR-406 fgf9 Fgf9 tropicalis pGEM-T amp Full Length

HAR-80 fgfr1 RTK FGFR TNeu049h18 n/a p tropicalis CS107 amp

HAR-336 fgfr1 XtFGFR1-107 TGas007c09 n/a p tropicalis CS107 amp Full length. Accession number AL633225

HAR-337 fgfr1 n/a XtFGFR1-107- FGFR1 tropicalis CS107 amp AL778654

HAR-341 fgfr1 n/a XtFR1isp7-1 FGFR1 in situ probe tropicalis CS107 amp AL778654

HAR-342 fgfr1 n/a XtFR1isp7-2 FGFR1 in situ probe tropicalis CS107 amp AL778654

HAR-77 fgfr2 xfgfr2, fgfr-2 RTK FGFR2 tropicalis CS107 amp Not full Length - Tgas066A10 - AL682450

HAR-115 fgfr2 xfgfr2, fgfr-2 RTK fgfr2 L1G8c12 n/a p tropicalis CS107 amp

HAR-122 fgfr2 xfgfr2, fgfr-2 RTK fgfr2 TGas066a10 n/a p tropicalis CS107 amp

HAR-338 fgfr2 xfgfr2, fgfr-2 n/a XtFGFR2part1 FGFR2 tropicalis CS107 amp AL637435

HAR-343 fgfr2 xfgfr2, fgfr-2 n/a XtFR2isp7 FGFR2 in situ probe tropicalis CS107 amp AL637435

HAR-346 fgfr3 n/a XtFGFR3-107 FGFR3 tropicalis CS107 amp AL964086

HAR-87 fgfr4 RTK FGFR4a TGas028m19 N/A p tropicalis CS107 amp AL652720 - Full Length - Tgas028m19

HAR-339 fgfr4 n/a XtFGFR4-107 FGFR4 tropicalis CS107 amp AL627929

HAR-340 fgfr4 n/a XtFGFR4-107- FGFR4 tropicalis CS107 amp AL652720

HAR-344 fgfr4 n/a XtFR4ispKS FGFR4 in situ probe tropicalis pBSKS amp AL652720

HAR-345 fgfr4 n/a XtFR4isp tropicalis CS107 amp AL652720

100 fli1 Xfli, fli, fli-1, Xfli1, Xfli-1 blood/vascular TF XFli1 X Fli1-pBS p p laevis Meyer-etal1993. Mech Dev 44: 109-121. pBluescript Amp

919 fli1 Xfli, fli, fli-1, Xfli1, Xfli-1 transcription factor Fli1-like Fli1-like (8548018) no pictures p laevis pCS111 Amp Image EST 8548018, Accession BC129781

HAR-302 TF n/a fli flightless1 tropicalis pCS108 amp CX342033

SM-1 flot1 flotillin 1 flotillin1, flotillin-1 signal transduction pBS-flotillin 1A’ pBS-flotillin 1A’ laevis pBSII-SK amp GenBamk Accession AF545660

SM-3 flot1 flotillin 1 flotillin1, flotillin-1 signal transduction pBS-flotillin 1B pBS-flotillin 1B laevis pBSII-SK amp GenBamk Accession AF545661

591 flt1 flt, vegfr1 growth factor receptor Flt1 like IMAGE:5542228 p laevis pCMV-SPORT6 Amp IMAGE EST 5542228, Accession number AAH44264

593 flt1 flt, vegfr1 receptor Flt-1 IMAGE:4959298 p laevis pCMV-SPORT6 Amp IMAGE EST 4959298, Accession number AAH56023

887 flt3 flk2, stk1, flt-3 tyrosine kinase Flt3-like Flt3-like (3401578) not in xenbase p tropicalis pCMV-SPORT6 Amp Image EST 3401578

589 flt4 vegfr3, vegfr-3, flt-4 Flt-4 IMAGE:4970772 p tropicalis pCMV-SPORT6 Amp IMAGE EST 4970772

HAR-64 fn1 fibronectin 1 Fibronectin IMAGE:4462773 n/a p tropicalis CS107 amp

fibroblast growth factor 9 (glia-

activating factor)GAF, FGF-9, HBGF-9,

XFGF-9heparin-binding growth

factorno accession

number

fibroblast growth factor receptor 1

X1FGFR, XFGFR-1, flt2, flt-2, xfgfr1, cek, flg, ogd, flt2, kal2, bfgfr,

fgfr-1

XB-CLONE-4319989

Not full Length - AL661849 Tneu049H18



fgfr-1

receptor tyrosine kinase

XB-CLONE-4133082



fgfr-1




fgfr-1




fgfr-1



not sure - clone HAR-122 has also this clone name


XB-CLONE-4454046

AL637435 - 3' presentDon't have 5'Clone Lig8c12


XB-CLONE-4157724

3' present - No 5'TGas066a10





fibroblast growth factor receptor 3 (achondroplasia,

thanatophoric dwarfism)

ach, cek2, jtk4, cd333, hsfgfr3ex, fgfr-3



FGF receptor 4, xfgfr4, XFGFR-4a, XFGFR-4,

fgfr-4XB-CLONE-4124588


GF receptor 4, xfgfr4, XFGFR-4a, XFGFR-4,

fgfr-4receptor tyrosine

kinase




kinase




kinase




kinaseFGFR4 in situ probe

ecoRI

Fli-1 proto-oncogene, ETS

transcription factor [provisional]

XB-CLONE-11903136



XB-CLONE-1557580

fli1 [provisional]



Xfli, fli, fli-1, Xfli1, Xfli-1

Pandur, P. D., M. L. Dirksen, K. B. Moore and S. A. Moody (2004). Xenopus flotillin1: a novel gene highly expressed in the dorsal nervous system. Developmental Dynamics 231: 881-887.Pandur, P. D., M. L. Dirksen, K. B. Moore and S. A. Moody (2004). Xenopus flotillin1: a novel gene highly expressed in the dorsal nervous system. Developmental Dynamics 231: 881-887.

fms-related tyrosine kinase 1


Gene]

XB-CLONE-2193836


resolved



Gene]

XB-CLONE-2228154


resolved


XB-CLONE-2356557


vascular endothelial growth factor receptor

XB-CLONE-2032957

have to check xenbase as images disapeared when uploaded them

mgc97508, gene C, fibronectin

extracellular glycoprotein

XB-CLONE-4456979

follistatin - BG886270 - uncertain - dad17g05.y1 Wellcome CRC pCS107 tropicalis St10-12 Silurana tropicalis cDNA clone IMAGE:4439985 5' similar to

TR:Q91376 Q91376 FOLLISTATIN.













Piccinni Maya:NO ORIGINALS


F243

Piccinni Maya: NO ORIGINALS

F244


HAR-112 foxa1 forkhead box A1 TF foxa1 TGas068h09 trops 14/17/19/28/31 p tropicalis CS107 amp TGas068H09 - AL681017

HAR-136 foxa2 forkhead box A2 TF n/a Foxa2 tropicalis CS107 amp AL779335 - Full length.

66 foxa4 forkhead box A4 pintallavis aPintallavis-BS no p laevis see Dev 116 81-93 for sense probe. Bluescript KS II amp

HAR-138 foxa4 forkhead box A4 TF n/a XFD-1 xfd1 foxa4a foxa4 tropicalis CS107 amp Full length CR762190

HAR-73 foxb1 forkhead box B1 fkh-5, fkh5, xfkh-5 TF Fkh5 TGas064p06 n/a p tropicalis CS107 amp

854 foxc1 forkhead box C1 TF FoxC1 IMAGE:7977266 have to upload them p laevis pExpress1 Amp Image EST 7977266, Accession BC108535

HAR-156 foxc1 forkhead box C1 TF n/a XFD11 xFD11 foxc1 fkhd tropicalis CS107 amp Full length AL967482 - Tgas132N18

HAR-165 foxc2 TF xFd4 TNeu106j02 n/a p tropicalis CS107 amp Tneu106J02 - AL788699

HAR-170 foxc2 TF XFD4 TNeu075j16 n/a p tropicalis CS107 amp AL776355 - Tneu075J16 - Full length

HAR-350 foxc2 n/a foxC2 tropicalis CS107 amp BX748240

HAR-425 foxc2 n/a FoxC2 FoxC2, forkhead C2 no accesion number tropicalis PCRII-TOPO amp

855 foxc2 FoxC2 IMAGE:7975505 have to upload them p laevis pExpress1 Amp Image EST 7975505

45-KL foxd1 forkhead box D1 XBF-2 CS105 FL XBF-2 not needed p laevis Mariani and Harland, Development 125 1998 CS105 Amp Full length XBF cDNA

HAR-83 foxd3 forkhead box D3 XFD-6, xfoxd3 TF Fkh6 TGas035o17 N/A p tropicalis CS107 amp Tgas035o17

HAR-216 foxd3 forkhead box D3 XFD-6, xfoxd3 TF FoxD3 IMAGE:6988210 trops 35 p tropicalis CS107 amp

HAR-158 foxd4l1.1 TF XFD12 TGas063b23 n/a p tropicalis CS107 amp Tgas063B23

HAR-160 foxd4l1.1 TF XFD-12 TGas055d21 Trops 24/28 p tropicalis CS107 amp Tgas055d21 - Full lengt

SM-11 foxd4l1.1 TF pCS2+MT-FoxD5 pCS2+MT-FoxD5 laevis

SM-7 foxd4l1.1 TF pBS-FoxD5 pBS-FoxD5 laevis Sullivan et al., 2001, Developmental Biology 232:439 pBS-SK amp

SM-8 foxd4l1.1 TF n/a pCS2-FoxD5 pCS2-FoxD5 laevis

HAR-272 foxf1 forkhead box F1 TF n/a XFD13 xfd13 FoxF1b fox tropicalis CS107 amp AL781142

HAR-275 foxf1 forkhead box F1 TF n/a XFD13 xfd13 FoxF1b fox tropicalis CS107 amp BX728208

HAR-227 foxg1 forkhead box G1 TF n/a XBF-1 xBF-1 xbf1 bf-1 bf1 fox tropicalis PCRII-TOPO amp not full length CX881474

HAR-166 foxi1 forkhead box I1 TF FoxI1 TEgg136p02 n/a p tropicalis CS107 amp Tegg136P02 - AL866461

HAR-171 foxi1 forkhead box I1 TF FoxI1 TNeu084p05 n/a p tropicalis CS107 amp AL780999 - TNeu084p05 - Not full length

HAR-176 foxi1 forkhead box I1 TF Forkhead box IMAGE:5382411 n/a p tropicalis pCMV-sport6.1 amp

HAR-162 foxi4.1 foxI1c, xfd-10 TF FoxI1c TGas113f01 unspecified stage p tropicalis CS107 amp Tgas113F01 - AL958990 - 3' Present

HAR-32 foxi4.2 XFD2', xlFox1a, foxia TF FoxI1a TGas002h16 n/a p tropicalis CS107 amp

xfkh2, HNF3alpha, HNF-3 alpha, HNF3 alpha, HNF3-alpha, tFoxA1, hnf3a, tcf3a

XB-CLONE-4155546

hnf3b, hnf3beta, hnf3-beta

fox foxa2 hnf3b hnf3beta

pintallavis, xfkh1, FoxA4a, XFD-1, xkfh-1,

fkh1forkhead domain

transcription factor XB-CLONE -

8596611Note - probably use shorter probe for RPA and longer

probe for in situ.

pintallavis, xfkh1, FoxA4a, XFD-1, xkfh-1,

fkh1 XB-CLONE-4152278

igda, ihg1, fkhl7, irid1, rieg3, freac3, freac-3

XB-CLONE-1661243

igda, ihg1, fkhl7, irid1, rieg3, freac3, freac-3,

xfd-11forkhead box C2

(MFH-1, mesenchyme forkhead 1)

fd-4, XFD-4, MFH-1, Mfh1

XB-CLONE-4251939

forkhead box C2 (MFH-1,

mesenchyme forkhead 1)


XB-CLONE-4305593




forkhead domain transcription factor



d-4, XFD-4, MFH-1, Mfh1




d-4, XFD-4, MFH-1, Mfh1


XB-CLONE-1659505

mgc89744, Xenopus brain factor 2, xbf-2,

BF-2, Xbf2forkhead domain

transcription factorXB-CLONE-12442428XB-CLONE-4127536

XB-CLONE-4400464

dbEST Id: 19434281EST name: AGENCOURT_15040932

GenBank Acc: CF220512GenBank gi: 33421220

CLONE INFOClone Id: IMAGE:6988210 (5')

forkhead box D4-like 1, gene 1

foxD5a, xlFoxD5a, xlFoxD5b, xtFoxD5,

foxd5, Xfd-12, FoxD5bXB-CLONE-4315487



foxd5, Xfd-12, FoxD5bXB-CLONE-4155404



foxd5, Xfd-12, FoxD5b, XFlip




complete coding region; Aka XFoxE4-pBS, XFlip1-2-2, or XFlip 1-2

GenBamk Accession AF162782




xfd-13, fkhl5, acdmpv, freac1


XBF-1, foxg1b, bf1, XBf1

Xema, ectodermally-expressed

mesendoderm antagon, FoxI1e, xfoxi1

XB-CLONE-4092727


mesendoderm antagon, FoxI1e, xfoxi1

XB-CLONE-4252726


mesendoderm antagon, FoxI1e,

xfoxi1, ema, foxi1e, foxi3

XB-CLONE-4442187

NISC_ng06a02.y1 NICHD_XGC_Emb6 Silurana tropicalis cDNA clone IMAGE:5382411 5', MRNA sequence

gi|21081568|gb|BQ393881.1|[21081568]

forkhead box I4, gene 1




xFD2 fox FoxI1a - 3' present - Tgas002H16 - Al595215Maybe have 5' of gene also














HAR-23 XFD2', xlFox1a, foxia FoxI1a TNeu012h23 trops 28 p tropicalis CS107 amp TNeu012h23 (5') - 3' present

HAR-175 XFD2', xlFox1a, foxia TF n/a forkhead fkhd forkhead fox tropicalis CS107 amp AL631579

HAR-173 foxj1 forkhead box J1 TF FoxJ1 IMAGE:5309205 n/a p tropicalis pCMV-sport6.1 amp

HAR-174 foxj2 forkhead box J2 mgc69377 TF FoxJ2 IMAGE:5335204 trops 28 p tropicalis pCMV-sport6.1 amp

406-NP foxk2 forkhead box K2 ILF1, foxk1 TF not in xenbase FoxK2 FoxK2 laevis pCMV SPORT6 Amp

HAR-167 foxq1 forkhead box Q1 n/a TF Foxq1 TNeu015l12 n/a p tropicalis CS107 amp Tneu015L1 - AL636071

HAR-172 foxr1 N/A TF FoxR1 TEgg019g15 trops 25 p tropicalis CS107 amp Tegg019G15 - AL853530

301 frzb wnt receptor FrzB-1 p laevis pBluescript SK

HAR-65 frzb Frzb IMAGE:4463392 trops 19/23 p tropicalis CS107 amp

HAR-72 frzb Frzb TGas069i22 n/a p tropicalis CS107 amp AL683768 - Tgas069i22

HAR-74 frzb Frzb TNeu037b10 n/a p tropicalis CS107 amp AL658040 - Tneu037b10

21-ER frzb xFrzb-HA pCS2-xFrzb-HA Cell 88, 747-756 pCS2+ Amp Wnt inhibition xFrzb-1, also called sFRP3 tagged

20-ER frzb Frzb1 pCS Frzb1 laevis Mech Dev 96, 183-195 pCS2+ Amp for sense and antisens un tagged

312 frzb2 XCrescent n/a p laevis pcDNA3 Amp

434 frzb2 FrzB2 no pictures p laevis Bradley et al, Dev Biol 227, 118-132 (2000) pCS2+ amp

12-ER Xcresent HA Xcresent HA not needed p laevis Mech Dev 96, 183-195 pCS2+ Amp

LJ-20 X Crescent 7 X Crescent 7 ? p laevis pGEMT Amp

HAR-150 n/a Crescent crescent frzb2 frzb-2 tropicalis CS107 amp Full length AL791100 - Tneu117G19

HAR-68 fst follistatin xfs, follistatin, foll follistatin IMAGE:4439985 n/a p tropicalis CS107 amp

8-CC fst follistatin xfs, follistatin TGFb modulator Follistatin Follistatin not needed p laevis pSP64TNE Amp Extracellular Regulators

HAR-403 fuz fuzzy homolog fuzzy n/a fuzzy tropicalis CS107 amp Full length

325-SPH fzd7 XFz7-CS2P+ XFz7-CS2P no pictures p laevis pCS2+ AMP

117-SPH fzd7 pGEMXfz7 (s/b) pGEMXfz7 - s-b no pictures laevis pGEM AMP Compleate ORF of Xfz7

146-SPH fzd7 pCS2+ xfz7 (S/B) pCS2xfz7 S-B not needed laevis

87-SPH fzd7 pBSXfz7(l1eii) pBSXfz7 - lambda1eii no pictures p laevis pBSK+ AMP complete open reading frame

123-SPH fzd8 Xfz8/pCS2 Xfz8-pCS2 not needed laevis Deardorff et al., Development 125, 2687 (1998) pCS2+ AMP Full length xfz8 PCR cloned into EcoRI/XhoI - af033110

108-SPH fzd8 pBS xfz8 cDNA pBS xfz8 cDNA no pictures p pBSII/KS AMP full length xfz8 cDNA - AF033110

539-AP fzr1 n/a XcdL1 XCdL1-CS2 not needed p laevis pCS2 Amp

16 gata1 n/a XGATA1A(a/s) XGATA1A(a/s), GP12 no laevis Bertwistle et al. pGEM7 Amp

1 gata2 n/a XGATA2 X GATA2B ,1.3 no tropicalis / laevis 1994 Development 120. 2519-2529 p GEM7 Amp 5' end of XGATA-2B

foxi4.2 [provisional]


[provisional]forkhead domain

transcription factorXB-CLONE-4338729

foxi4.2 [provisional]


[provisional]

xfoxj1, xfoxj1', hfh4, hfh-4

XB-CLONE-4444512

THIS CLONE IS A DUPLICATE OF 191??? NISC_mq24c11.y1 NICHD_XGC_Emb5 Silurana

tropicalis cDNA clone IMAGE:5309205 5', MRNA sequence

gi|21080102|gb|BQ392415.1|[21080102] NISC_mq24c11.x1 NICHD_XGC_Emb5 Silurana

tropicalis cDNA clone IMAGE:5309205 3', MRNA sequence

gi|21080101|gb|BQ392414.1|[21080101]

XB-CLONE-4435944

NISC_nl03a03.y1 NICHD_XGC_Emb7 Silurana tropicalis cDNA clone IMAGE:5335204 5', MRNA sequence

gi|21378989|gb|BQ520120.1|[21378989]

XB-CLONE-4343287

forkhead box R1 [provisional]

XB-CLONE-4111594

frizzled-related protein

Frzb-1, xfrzb, fre, os1, fzrb, fiz, frp-3, frzb1, sfrp3, srfp3, frzb-pen

XB-CLONE-12271629



Frzb-1, xfrzb, fre, os1, fzrb, fiz, fritz, frp-3,

frzb1, sfrp3, srfp3, frzb-pen


XB-CLONE-4457437

Frzb - Genbank Accession #BG886039 - Full Lengthnot BamH1 - HindIII or R1 OK





XB-CLONE-4117524





XB-CLONE-4325971



secreted wnt antagonist

Xenopus laevis Frzb-1 (Frzb-1)




frizzled-related protein 2

[provisional] crescent, frizzled related protein


XB-CLONE-12271684

Pera EM and DeRobertis EM (2000), Mech. Dev. 96 183-195


[provisional] crescent, frizzled

related proteinsecreted wnt antagonist

XB-CLONE-12441167

frzb2 [provisional]


[provisional]crescent, frizzled related protein


XB-CLONE-8851989

frzb2 [provisional]


[provisional]crescent, frizzled related protein


XB-CLONE-11878849

frzb2 [provisional]


[provisional] crescent, frizzled related protein


activin and bmp7 antagonist, also

antagonizes bmp2/4 but less effectively

XB-CLONE-4465154

CRC pCS107 tropicalis St10-12 Silurana tropicalis cDNA clone IMAGE:4439985 5' similar to TR:Q91376 Q91376

FOLLISTATIN.BG348597

XB-CLONE-12442174

no accession number

frizzled family receptor 7

Fz-7, Xfz7, frizzled-7, frizzled7, frizzled 7,

frz7, frz-7, fz7wnt signalling


PCR Amplified coding region (BamHI-ZbaI) of Xenopus Frixxled-7.










bug is resolved





Two novel Xenopus frizzled genes expressed in developing heart and brain. Wheeler and hoppler Mech. Dev. 86 1-2 203-207


Xfz8, Fz-8, frizzled-8, frizzled 8, frizzled8


XB-CLONE-14574078


bug is resolved


Xfz8, Fz-8, frizzled-8, frizzled 8, frizzled8


XB-CLONE-11536127


bug is resolved

fizzy/cell division cycle 20 related 1

anaphase promoting complex component

XB-CLONE-12442115

GATA binding protein 1 (globin

transcription factor 1)

xGATA-1, xgata1, gata-1

zinc finger transcription factor

When growing bugs transformed with any GATA add glucose (0.1%) to plates or culture media.

GATA binding protein 2

xGATA-2, GATA-2, xgata2













add as gene sinonym-Maya Piccinni

not in xenbase yet-Maya Piccinni

G297

add as gene sinonym -Maya Piccinni

K307

not in xenbase yet -Maya Piccinni

9 gata2 n/a pSP64TGATA2 pSP64T-GATA2a not needed laevis For sequence Zon et al (1991) PNAS 88:10642 pSP64T Amp

132 gata2 pBut2xGATA2enMT not needed p laevis pBut2enMT AMP

148 gata2 blood/neural Tf G2fVP16 G2fVP16 not needed p laevis But2VP16 amp

82 gata3 Blood TF pBUTGATA3 pBUTGATA3 p p laevis pBUT2 Amp globin UTRs

155 gata4 pskXG4 p need re doing p laevis Developmental Biology 174pp258-270 blueskript II SK (+/-) Amp

157 gata4 heart/gutTF pXGATA4 pT7TS not needed p laevis pT7TS(R1+)

351 gata4 heart/gut TF XGATA4-GR-HA not needed p laevis pSp64T Amp

370 gata4 heart/gut TF XGATA4-HA-pBut2 not needed p laevis pBut2 chloramphenicol

371 gata4 heart /gut TF XGATA4-HA-pBut3 not needed p laevis pBut3 chloramphenicol

458 gata4 XGATA4-HA-pBut3-L not needed p laevis pBut3 Amp

HAR-258 gata4 heart/gut TF n/a Gata-4 gata4 gata-4 tropicalis CS107 amp AL960651

HAR-259 gata4 heart/gut TF n/a Gata-4 gata4 gata-4 tropicalis CS107 amp AL963806

21 gata5 n/a XGATA5amyctag pButXG5aMT laevis pBut2 Amp

101 gata5 pBut2xGATA5EnMT not needed p laevis pBut2enMt AMP

156 gata5 heart/gut TF pskXG5 p p laevis Developmental Biology 174pp258-270 bluesript II SK (+/-) Amp

352 gata5 heart/gut TF XGATA5-GR-HA not needed p laevis pSp64T Amp

372 gata5 heart/gut TF XGATA5-HA-pBut2 not needed p laevis pBut2 Amp

502 gata5 heart/gut TF XGATA5-HA-pBut2-L not needed p laevis pBut2HA Amp

503 gata5 heart/gut TF XGATA5-HA-pBut3-L not needed p laevis pBut3HA Amp

778 gata5 Gata5a IMAGE:3400525 no pictures p laevis pCMV-Sport6 Amp Image EST 3400525

20 gata5 n/a pSP64TxG5(as) pSP64TxG5(as) laevis pSP64T Amp

HAR-245 gata5 gut/heart TF n/a gata-5 gata5 gata-5 endoderm tropicalis pCMV-SPO amp CF220508

193 gata6 xG6 580 G6580 p laevis Gove et al (1997) EMBO J 16, 355-368 pGEM3Z Amp 3'UTR

288 gata6 GATA6sengrailed not needed p laevis pBUT2-EnMT Amp

302 gata6 GATA6S VP16 not needed p laevis But2 VP16




Note the EcorI site in the middle of GATA-2 cDNA has been mutated making it possible to use EcorI to

linearise the plasmid.




XB-CLONE-11928879

Gata2 was PCRed out of psp64T and is missing an Eco RI site at 1318bp of the Gata 2 cDNA. So you can

linearize with EcoRI GATA binding

protein 2 xGATA-2, GATA-2,

xgata2 XB-CLONE-12271658


GATA-3, XGATA-3, XGATA3

XB-CLONE-11536596


xGATA-4, gata-4, xgata4


XB-CLONE-12025634



XB-CLONE-12033340



XB-CLONE-12440869



XB-CLONE-12440906



XB-CLONE-12440909




XB-CLONE-12441185






xGATA-5, xGATA5, xGATA-5a, xGATA5a, xGATA-5b, xGATA5b,

GATA-5, gata-4a




GATA-5, gata-4a


XB-CLONE-11917134



GATA-5, gata-4a

XB-CLONE-12030392



GATA-5, gata-4a

XB-CLONE-12440872



GATA-5, gata-4a

XB-CLONE-8596618



GATA-5

XB-CLONE-12441207



GATA-5

XB-CLONE-12441210



GATA-5


XB-CLONE-2313585



GATA-5, gata-4a




GATA-5, gata-4a


GATA-6, XGATA-6, XGATA6, tGata6, gata6b, gata6a


XB-CLONE-12055295

have pictures but can not be added till have

probe info




XB-CLONE-12271622




XB-CLONE-12271729














425 gata6 heart/gut TF XLongGATA6-GR-HA not needed p laevis pSP64T Amp

HAR-111 gata6 gut/heart TF gata6 gata gata-6 n/a p tropicalis pCMV-SPORT6.1 amp

HAR-453 gata6 Gata6 IMAGE:7722828 no pictures p tropicalis CS107 amp

HAR-454 gata6 Gata6 IMAGE:7676570 no pictures p tropicalis pcmvSport6 amp Accession number CX889517.1

HAR-455 gata6 Gata6 IMAGE:7523548 no pictures p tropicalis CS108 amp CX473127.1

760 gata6 XlGATA6 (MDL) HA not needed p laevis pBUT3HA Amp long GATA6 in pBUT3HA

2 gata6 n/a XGATA6 no laevis Gove et al. 1997; Embo Journal, Vol.16, No2, pp 355-368 Bluescript SK +/- Amp

HAR-82 gbx2.2 XGBX-2 TF Gbx2 TGas027p15 trops 13/15/19/21/28 p tropicalis CS107 amp AL647208 - Tgas027p15

HAR-89 gbx2.2 XGBX-2 TF Gbx 2 TNeu059g14 N/A p tropicalis CS107 amp AL675475 - Tneu059g14

HAR-331 n/a GTP cyclohydrolase I n/a GTP GTP cyclohydrolase gch tropicalis pExpress1 amp BC075602

783 gcm1 n/a Gcm1a IMAGE:5570938 no pictures p laevis pCMV-Sport6 Amp Image EST 5570938. Full length. Accession BC043988

HAR-201 gdf1 signalling protein Vg1 TEgg053l11 trops 32 p tropicalis CS107 amp Not full Length - Tegg053L11 - AL872984

365 gdf3 Derriere (Jones) XB-IMG-48384 p laevis Sun et al. (1999). Development 126: 1467-1482. CS2+ Amp

368 gdf3 Derriere-Sive n/a p laevis CS2 amp

HAR-69 gdf3 , rps3a signalling molecule derriere TGas141f11 trops 11/17 p tropicalis CS107 amp

595 gdf6 receptor pBS-GDF6 no images p laevis Chang & Hemmati-Brivanlou 1999. Dev 126: 3347-3357 pBS Amp

HAR-289 n/a GDF6 CS107 GDF6 tropicalis CS107 amp Full length AL968587

858 gfi1 n/a Gfi1a IMAGE:8547327 have to upload them p tropicalis pCS111 Amp Image EST 8547327

859 gfi1b Gfi1b IMAGE:5506836 have to upload them p laevis pCMV-SPORT6 Amp

779 gfl7 mgc53105, xegfl7 calcium ion binding Egfl7 IMAGE:5542405 yes p laevis pCMV-Sport6 Amp Image EST 5542405

849 gja1 connexon component Cx43 Cx43 5514939 have to upload them p laevis pCMV-SPORT6 Amp Image EST 5514939, Accession BC073407

848 gja3 connexon component Cx40.4 Cx40.4 4031512 have to upload them p laevis pCMV-SPORT6 Amp Image EST 4031512

846 gja4 connexin 37, cx37 Cx37 IMAGE:5512615 have to upload them p laevis pCMV-SPORT6 Amp Image EST 5512615, Accession BC073347

884 gja4 connexin 37, cx37 gap junction protein Cx41 Cx41 (4969189) no pictures p laevis pCMV-SPORT6 Amp Image EST 4969189

850 gjc1 connexin 45, cx45, gja7 connexon component Cx45 IMAGE:4683883 have to upload them p laevis pCMV-SPORT6 Amp Image EST 4683883, Accession BC042343

559 gli1 Gli-1 neural TF Gli1 XGli1-1/pBluescript p laevis Takabatake et al. 2000. Mech Dev 98: 99-104 pBluescript II SK- Amp Contains cDNA fragment 2276-4081



XB-CLONE-8596622


GATA-6, XGATA-6, XGATA6, tGata6,

gata6bXB-CLONE-4439106

BQ395911 - dbEST Id: 12440736EST name: NISC_ng17h04.y1

GenBank Acc: BQ395911GenBank gi: 21083598


Source: IMAGE Consortium, LLNLPlate: LLAM11977 Row: O Column: 7



gata6bzinc finger transcription














XL GATA6 MDL HA pBut3




PXG6 -3.3 (ZEUS)pXG 6 - 3.3 (ZEUS)

gastrulation brain homeobox 2, gene

2XB-CLONE-4130524

gastrulation brain homeobox 2, gene

2XB-CLONE-4264085

gch1 [Predicted: Source is

Human Entrez Gene]

GTP cyclohydrolase 1 [Predicted:


glial cells missing homolog 1

transcriptional regulator

XB-CLONE-2192932

growth differentiation

factor 1vg1, vg-1, dvr1, dvr-1,

gdf-1XB-CLONE-4083918


factor 3 derrière, derriere, vgr2, vgr-2, gdf-3


XB-CLONE-12440892


factor 3derrière, derriere, vgr2,

vgr-2, gdf-3tgf-beta related

peptide growth factorXB-CLONE-12440899

growth differentiation factor 3 and

ribosomal protein S3A [Predicted:


derrière, derriere, vgr2, vgr-2, gdf-3 and

fte1, mftl XB-CLONE-3992793

Tgas141F11 - not full length*tested for in situ - works


factor 6 [Predicted: Source is Human

Entrez Gene]

kfs, kfsl, sgm1, bmp13, cdmp2, gdf-6

XB-CLONE-12441315

gdf6 [Predicted: Source is

Human Entrez Gene]


factor 6 [Predicted: Source is Human

Entrez Gene]

kfs, kfsl, sgm1, bmp13, cdmp2, gdf-6

TGFb signalling modulator

growth factor independent 1 transcription

repressor

neural transcription repressor

XB-CLONE-1557476

growth factor independent 1B

transcription repressor

znf544, zinc finger protein 544

neural transcription repressor

XB-CLONE-2042654

Image EST 5506836, Accession BC077878. Also called Znf544

EGF-like-domain, multiple 7

XB-CLONE-2191834

connexin 43, connexin43, cx43,

alpha 1 gap junction protein

XB-CLONE-2036594

connexin 46, cx46, alpha3 connexin,

Cx40.4XB-CLONE-2112494

gap junction protein, alpha 4,

37kDaconnexon channel

componentXB-CLONE-2048712

gap junction protein, alpha 4,

37kDa XB-CLONE-2206125

XB-CLONE-2193584

GLI family zinc finger 1

XB-CLONE-12441250

sent 7 pictures to xenbase on 5.04.13













have to add as synonim of gene-Maya Piccinni



G345

have to add as synonim of gene -Maya Piccinni

I355


F359


450-AP gli1 Gli-1 transcription factor XGli1 pGEMT-Gli1 no pictures p laevis Ruizi Alteba, EMBL ccc Nr U57454 pGEMT Amp

560 gli3 XGli-3, Xgli3 Gli3 Xgli3-3 p laevis Takabatake et al. 2000. Mech Dev 98: 99-104 pGEM-T Amp Contains cDNA fragment 2251-2902

451-AP gli3 XGli-3, Xgli3 transcription factor XGli3 pCS2plusMT XGLi3 no pictures p laevis pCS2+MT Amp 4747bp (ORF)

452-AP transcription factor not in xenbase Xgli4 pCS2plusMT XGLi4 not needed p tropicalis pCS2+MT Amp 4081 (ORF)

777 n/a Glomulin IMAGE:5049118 yes p laevis pCMV-Sport6 Amp Image EST 5049118

252-NP geminin, gem GTPase Gem L Gem L n/a p laevis CS2+ Amp

HAR-308 geminin, gem n/a gmnn geminin tropicalis pCMV-SPO amp CF218367

875 n/a platelet surface protein Gp1bb IMAGE:4965713 have to upload them p laevis pCMV-SPORT6 Amp Image EST 4965713. Accession BC072988

882 gp9 gpix, cd42a platelet surface protein GlycoIX no pictures p laevis pCMV-SPORT6.ccdb Amp Image EST 6946300. Accession BC080112

920 gpbp1 n/a transcription modulator Vasculin Vasculin (5155594) no pictures p laevis pCMV-SPORT6 Amp Image EST 5155594, Accession BC077810

25-ER gpc4 glypican 4 cell surface protein Glypican 4 pCS2 - Glypican 4 laevis Int J Dev Bio 49,781-796 pCS2+ Amp Xenopus Glypican 4 full length

HW-16 grip2 Xgrip2, Xgrip2.1 PDZ domain n/a pCS2Grip2 MO- pCS2Grip2 MO- laevis pCS21 amp

HAR-38 gsc Xgsc, goosecoid TF Goosecoid tgas02869 n/a p tropicalis CS107 amp

HAR-39 gsc Xgsc, goosecoid TF Goosecoid TGas129e16 trops 12/12/20/28 p tropicalis CS107 amp

HAR-40 gsc Xgsc, goosecoid TF Goosecoid TGas028b09 trops 12/12/20/28 p tropicalis CS107 amp

HAR-42 gsc Xgsc, goosecoid TF Goosecoid tgas091013 n/a p tropicalis CS107 amp Tgas091013 - 3' present

HAR-43 gsc Xgsc, goosecoid TF Goosecoid TGas075o13 n/a p tropicalis CS107 amp Tgas075o13 - AL776879 - 3' present

HAR-46 gsc Xgsc, goosecoid TF Goosecoid TNeu077f20 n/a p tropicalis CS107 amp Tneu077F20 -AL777938 - 3' present

24-ER gsc Xgsc, goosecoid TF xGoosecoid-FL xGoosecoid-FL laevis Cell 67,1111-1120 pCS2+ Amp

63-KL gsc Xgsc, goosecoid not known pG500 pG500 no pictures p laevis pBSII KS Amp goosecoid typeB

175-SPH gsk3a xgsk3 XG73 (xGSK3) xG73 not needed p laevis pCS2+ AMP

HAR-419 hand2 dhand, dhand2, xhand2 n/a Hand 2 protein no pics tropicalis pcs108 amp Full length - AAI35784

HAR-277 hand2 TF n/a dHAND dHAND tropicalis CS107 amp CF218564

253-NP has1 Xhas1, dg42 metabolic enzyme hyaluronan sythase I n/a p laevis pBS SK(-) Amp


XB-CLONE-14575029

EXRC CLone Number AP-450. DEFINITION Xenopus laevis zinc finger DNA binding protein Gli-1 mRNA,

partial cds. ACCESSION U57454. 1464bp partial fragment of XGliI (nucleotides 866-2329).


Hedgehog dependent TF

XB-CLONE-12441253



XB-CLONE-12442128

A role for Xenopus Gli-type zinc finger proteins in the early embryonic patterning of mesoderm and neuroectoderm.Marine JC, Bellefroid EJ, Pendeville H, Martial JA, Pieler T.Mech Dev. May 1, 1997; 63 (2): 211-25.

Gli4 gene not in xenbase http://www.ncbi.nlm.nih.gov/gene?db=gene&cmd=Retrieve&dopt=full_report&list_uids=397838

A role for Xenopus Gli-type zinc finger proteins in the early embryonic patterning of mesoderm and neuroectoderm.Marine JC, Bellefroid EJ, Pendeville H, Martial JA, Pieler T.Mech Dev. May 1, 1997; 63 (2): 211-25.

glmn [Predicted: Source is

Human Entrez Gene]

glomulin, FKBP associated protein [Predicted: Source is Human Entrez

Gene]

Uncharacterized conserved protein

XB-CLONE-2217447

gmnn [provisional]

geminin, DNA replication inhibitor


gmnn [provisional]

geminin, DNA replication inhibitor

[provisional] DNA replication

inhibitor

gp1bb [Predicted: Source is

Human Entrez Gene]

glycoprotein Ib (platelet), beta

polypeptide [Predicted: Source is Human Entrez

Gene]

XB-CLONE-2207733

glycoprotein IX (platelet)


Gene]

XB-CLONE-2077396

Glycoprotein IX (6946300)

GC-rich promoter binding protein 1


Gene]

XB-CLONE-2149295

K-glypican, Xgly4, glypican-4, knypek

glutamate receptor interacting protein

2

Kirilenko, P., Weierud, F. K., Zorn, A. M. and Woodland, H. R. (2008). The efficiency of Xenopus primordial germ cell migration depends on the germplasm mRNA encoding the PDZ domain protein Grip2. Differentiation 76, 392-403.

A construct was made to express Grip2 in the presence of the MO used by Kirilenko et al.. The ORF of Grip2

without the MO-binding sites and carrying a stop codon was amplified by PCR and was cloned into the ClaI and

BamHI sites of a pCS21expression vector using the primer 2LG2MOminF 50-

ATATAGAATTCGCCACCATGCATTTTTTCCAGACCATTCTTA-30, introducing a BamHI site, and

the primer 2LG2stopR 50-TATATATCGATTCAGAGAGTCTTTG TGCTTGTCT-30,

introducing a ClaI site. RNA was synthesized from NotI-linearized plasmid with SP6 RNA.

goosecoid homeobox

XB-CLONE-14575063

Tgas02869 - Al652832 - 3' presentLikely have 5' also

goosecoid homeobox

XB-CLONE-4032957

Tgas129E16 - AL965382 - 3' presentLikely have 5' also*Works for in situ

goosecoid homeobox

XB-CLONE-4124404

Tgas028B9 - AL652832 - 3' presentMost likely have 5' end

goosecoid homeobox

XB-CLONE-14575065

goosecoid homeobox

XB-CLONE-4115278

goosecoid homeobox

XB-CLONE-4266151

goosecoid homeobox

goosecoid homeobox

XB-CLONE-12442463

Organizer-specific homeobox genes in Xenopus laevis embryos.

Blumberg B, Wright CV, De Robertis EM, Cho KW.Science. July 12, 1991; 253 (5016): 194-6.

glycogen synthase kinase 3 alpha


XB-CLONE-14574118

heart and neural crest derivatives

expressed 2basic helix-loop-helix transcription factor

heart and neural crest derivatives

expressed 2 dhand, dhand2,

xhand2 hyaluronan synthase 1

XB-CLONE-12442377

hyaluronan sythase I (103)















have to be uploaded-Maya Piccinni



I366

have to be uploaded -Maya Piccinni

F370


F371


28-ER has1 Xhas1, dg42 not in xenbase Hyaluronan Synthetase Hyaluronan Synthetase laevis Int J Dev Biol 49, 781-796 pCS2+ Amp Hyaluronan Synthetase-full length

CS-128 hdac1 retinoid receptor not in xenbase RARa2-1 RARa2-1 laevis pBluescript Amp EcoRI fragment

127-NP esr6e, esr-6e TF ESR 6e SK- ESR6eSK- no pictures p pBluescript SK- Amp

227 hes4 neural crest TF Hairy 2A hairy2A p laevis Toby's thesis

336-AP hes6.1 XHes6, Xhes-6 TF Xhes6 Xhes6 tropicalis pCS2+ Amp

HAR-119 hes7.2 TF n/a ESR4 no pics tropicalis CS107 amp

HAR-70 TF esr4 TNeu125h16 n/a p tropicalis CS107 amp

HAR-144 hey1 TF hey1 TEgg121i16 p tropicalis CS107 amp Accession number AL873208

HAR-213 hey1 TF hey2 IMAGE:6980422 p tropicalis pCMV-SPORT6.1 amp

153 hhex XHex, tHex, hex anterior Tf hex Xhex p laevis

HAR-63 hhex XHex, tHex, hex TF xHex IMAGE:4465000 n/a p tropicalis CS107 amp

HAR-107 hhex XHex, tHex, hex TF xHex TGas075h17 n/a p tropicalis CS107 amp AL776914 - TGas075h17

JW-1886 fluorescent protein CS2-H2BeGFP egfp seq pCS2+ Amp

541 hnf1b HNF1 homeobox B Hepatic TF XHNF1-beta pXLFB3 p laevis pGEM-7Zf- Amp

895 hoxa5 homeobox A5 hox1c, hox1.3 HoxA5 HoxA5 5515839 no pictures p tropicalis pCMV-SPORT6 Amp Image EST 5515839

921 hoxb2 homeobox B2 n/a HoxB2 HoxB2 (7977178) no pictures p laevis pExpress1 Amp Image EST 7977178, Accession DQ116736

HAR-187 hoxb9 homeobox B9 xhoxb9 TK HoxB9 IMAGE:6996089 trops 13-19-28-33/34 p tropicalis pCMV-Sport6.1 amp

HAR-221 hoxb9 homeobox B9 xhoxb9, hox 2.5 TF n/a HoxB9 hoxb9 tropicalis pCMV-SPO amp Full length - BQ393597

35-ER hoxd1 homeobox D1 TF not in xenbase xLabial xLabial laevis Science 253,194-196 Amp Xenopus HoxD1 probe

HAR-11 hoxd10 homeobox D10 TF HoxD10 TNeu001k05 trops 18-19-26-33-34 p tropicalis CS107 amp

HAR-20 hoxd10 homeobox D10 TF HoxD10 Tneu009D23 no pics p tropicalis CS107 amp 5' present

HAR-249 hrg hrgp, fetuinish Zn transporter n/a Fetuinish Fetuinish Cystatin tropicalis pExpress amp CF593173

HAR-460 id2 xid2 Id2 IMAGE:5382465 no pictures p tropicalis CS107 amp BC059758. Image 5382465. inhibitor of differentiation

907 id2 xid2 Id2 IMAGE:4971053 no pictures p laevis pCMV-SPORT6 Amp Image EST 4971053, Accession BC072843

HAR-97 id2 xid2 transcription modulator ID2 tropicalis CS107 amp CR855790

hyaluronan synthase 1

Chitin synthase/hyaluronan

synthase (glycosyltransferases)

histone deacetylase 1

HD1, rpd3, gon-10, rpd3l1

hes3.1 [provisional]

hairy and enhancer of split 3, gene 1


Xenopus laevis hairy and enhancer of split 3,

gene 1 (hes3.1)

Xhairy2, hairy2, hairy2b, Xhairy2b, hairy2a, XHairy2a,

hes4a, hes4b

helix-loop-helix transcription

factor/transcriptional repressor

XB-CLONE-12271672

have pictures but can not be added till have

probe info

hairy and enhancer of split 6,

gene 1

hes family bHLH transcription factor

7 [provisional] esr4, ESR-4, enhancer-

of-split-related 4AL789214- 3' present - 5' not present

TNEU138e11

hes7.2 [provisional]

hairy and enhancer of split 7, gene 2

[provisional]esr4, ESR-4, enhancer-

of-split-related 4XB-CLONE-4227621

Likely have 3', Don't have 5' - TNeu125H16 - Uncertain Length

hairy/enhancer-of-split related with

YRPW motif 1hrt1, XHRT1, chf2, hrt-1, hesr1, herp2, oaf1

XB-CLONE-4083543

trops unspecified stage/17/25

hairy/enhancer-of-split related with

YRPW motif 1hrt1, XHRT1, chf2, hrt-1, hesr1, herp2, oaf1

XB-CLONE-4414096

trops unspecified stage x2 /19/21/22




hematopoietically expressed homeobox

XB-CLONE-11536462

p need re doing late stages

hematopoietically expressed homeobox

XB-CLONE-4459002

Hex xhex - Genbank #BG815569 - full lengthNote this clone is in reverse orientation so that the 5'

end is at the Not I site andthe 3' end is at the RI site

This probe never worked for in situhematopoietically

expressed homeobox

XB-CLONE-4115222

hist2h2be [Predicted: Source is

Human Entrez Gene]

histone cluster 2, H2be [Predicted: Source is Human

Entrez Gene]

h2b, h2bq, gl105, h2b.1, h2bfq

Histone-2B - fused in frame to eGFP

Nuclear/chromosome label. live cell nuclear marker. Cloned blunt into XhoI/XbaI sites of CS2

vhnf1, hnf1beta, hnf-1beta, tcf2, hnf1-beta,

lfb3 XB-CLONE-12441229


homeodomain transcription factor

XB-CLONE-2031766


XB-CLONE-1644350

XB-CLONE-4386888

AGENCOURT_15100128 NICHD_XGC_Emb6 Silurana tropicalis cDNA clone

IMAGE:6996089 5', MRNA sequence gi|33441773|gb|CF238565.1|[33441773]

xlab, mgc131028, xhox.lab1, Xhox.lab,

xhoxd1, Xlab-1

Xhoxd10, hox4, hox4d, hox4e, hox-4.4

XB-CLONE-4349053

HoxD10 hoxd10 - Full Lenght - AL634532 - Tneu001K5Been tested for in situ - on trop website

Xhoxd10, hox4, hox4d, hox4e, hox-4.4

XB-CLONE-14575058

histidine-rich glycoprotein

inhibitor of DNA binding 2,

dominant negative helix-loop-helix

protein


XB-CLONE-4442148



protein


XB-CLONE-2033658



protein

accession number refers

to dazap2 gene
















F401


HAR-54 id3 xid3, heir-1, xidia transcription modulator ID3 TGas044c15 n/a p tropicalis CS107 amp

912 id3 xid3, heir-1, xidia, xIdx Id3 Id3 (5572155) p laevis pCMV-SPORT6 Amp Image EST 5572155, Accession BC044039

HAR-459 xid3, heir-1, xidia Id3 IMAGE:7785460 no pictures p tropicalis CS108 amp CX316420, IMAGE 7785460. inhibitor of differentiation

117-KL xid3, heir-1, xidia transcription modulator Id3-codL cs107-Id3-CodL no pictures p pCS107 Amp XId3

HAR-424 idb4, bhlhb27 n/a Id4 no accesion number tropicalis PCRII-TOPO amp

775 idb4, bhlhb27 transcription modulator Id4 IMAGE:3580163 no pictures p laevis pBSRN3 Amp Image EST 3580163

118-KL idb4, bhlhb27 transcription modulator Id4-N3 CS107 Id4-N3 no pictures p tropicalis pCS107 Amp 2.4Kb clonded from EST 3399628

390-AP igf1 signalling molecule XIGF-1 Xe IGF-1 laevis Amp

33-ER igf1r receptor protein kinase not in xenbase DN xlGFR DN xlGFR laevis Dev Cell 1,655-665 pCS2+ Amp

30-ER igf2 signalling molecule not in xenbase IFG2 laevis Mech Dev 116, 169-172 pCS2+ Amp

393-AP igf2 growth factor not in xenbase XIGF-2 XIGF-2 laevis pcMV-SPORT6 Amp Clone Image id 3396829

748 ihh indian hedgehog signalling molecule Xbhh Xbhh-pBluescript SK - have to upload them p laevis pBluescript SK (-) Amp cDNA X1L

HAR-243 impdh2 imp2 mitochondrial protein n/a Imp2 Imp2 Inosine-50 tropicalis pCMV-SPO amp CF222149

438 inhbb growth factor Activin B not needed p laevis pSPJC2L amp

1-CC inhibin, activin B growth factor Activin-betaB Activin-betaB not needed p laevis pSP64T Amp tgf-beta ligands

AP-69 signalling molecule Activin bb XActivin bb laevis psp64t Amp

HAR-71 intu transcription modulator pdzd6 TGas012e01 trops 28 p tropicalis CS107 amp

258-NP xirg not known XIRG (94) XIRG n/a p laevis pBS SK(-) Amp

148-NP irx1 Xiro, Xiro1 TF not in xenbase pBS Xiro I Probe pBS Xiro I Probe laevis pBS Amp

298-SPH irx2 mgc89388, Xiro2 TF Xiro2 Xiro2 no pictures laevis ? AMP

HAR-106 irx3 TF irx3 IMAGE:5383093 p tropicalis pCMV-SPORT6.1 amp BQ394718 - Full length - Image 5383093

HAR-123 irx3 TF irx3 IMAGE:5335930 n/a p tropicalis pCMV-SPORT6.1 amp Full length Clone Image 5335930 *Works for in situ

HAR-464 irx3 n/a Irx 3 tropicalis

299-SPH irx3 TF Xiro3 Xiro3 no pictures laevis ? AMP




XB-CLONE-4272575

CR761665 - UNCERTAIN - Also in Harland database - number 1789CS107-4.1 kb






XB-CLONE-2201006


id3 [provisional]





XB-CLONE-2942740

id3 [provisional]




XB-CLONE-12442507

laevis (8th down form list)

id4 [provisional]





Id4, inhibitor of differentiation 4

id4 [provisional]




XB-CLONE-2315325

id4 [provisional]




XB-CLONE-12442511

insulin-like growth factor 1

(somatomedin C)

IGF-I, Somatomedin, xigf1, igf-1,

somatomedin-C

insulin-like growth factor 1 receptor

xIGF-1R, xIGFR, xigf1r, igf-1r

dominant negative Insulin-like growth factor receptor (extracellular domain)


(somatomedin A) IGF-II, insigf, pp9974,

igf-2x Insulin Growth Factor

2


(somatomedin A) IGF-II, insigf, pp9974,

igf-2

X-BHH, banded hedgehog, bhh, X-hh,

indian hedgehog, XbhhXB-CLONE-12441520


IMP (inosine 5'-monophosphate) dehydrogenase 2

inhibin, beta B [provisional]

inhibin, activin B, activin beta b

XB-CLONE-12441171

inhbb [provisional]


XB-CLONE-12442156

inhbb [provisional]


inhibin, activin B, activin beta b

inturned planar cell polarity protein

pdzd6, int, inturned, PDZ domain containing

6, XintXB-CLONE-4145380

TGas012e01 Genbank Accession # AL629224 - Not full length

irg1 [provisional]

immunoresponsive gene 1


iroquois homeobox 1

iroquois homeobox 2

XB-CLONE-14574184


bug is resolved

iroquois homeobox 3

xiro3, mgc131103, xiro-3, irx3b

XB-CLONE-4440913

trops 13/14/15/18/20/21/22/2

3/27/28iroquois homeobox

3xiro3, mgc131103,

xiro-3, irx3bXB-CLONE-4434768

iroquois homeobox 3



iroquois homeobox 3


XB-CLONE-14574187


bug is resolved














300-SPH irx4 iroquois-4, irxa3 TF Xiro4 Xiro4 no pictures laevis ? AMP

301-SPH irx5 iroquois-5 TF Xiro5 Xiro5 no pictures laevis ? AMP

735 isl1 neural TF IsletI IsletI p laevis pCS2+ Amp Image EST 4058865

HAR-142 isl1 TF isl1 TNeu086d13 p tropicalis CS107 amp Accession number AL803057

HAR-418 isl1 Islet 1 IMAGE:7623033 no pics p tropicalis CS108 amp Full length - BC135764 - 7623033

588-SPH isl1 TF pBSxislet1 pBSxislet1 no pictures p laevis pBSSKII AMP

1087-AP isl1 TF XlisletI XL-isletI no pictures p laevis pCS105 Amp

34-ER isthmin immunoglobulin related not in xenbase pCS Isthmin pCS Isthmin laevis Mech Dev 116, 169-172 pCS2+ Amp

784 jag1 jagged 1 Serrate IMAGE:6634431 yes p laevis pCMV-Sport6 Amp Image EST 6634431, also called Jagged1

893 jun jun proto-oncogene AP-1, c-Jun cJun IMAGE:6957041 no pictures p laevis pCMV-SPORT6.1 Amp

592 Erg-1 IMAGE:5512670 no images p laevis pCMV-SPORT6 Amp IMAGE EST 5512670, Accession number AAH73350

HAR-294 RTK n/a kdr flk1 vegfr2 flk-1 tropicalis pCS108 amp CX341075

266-NP structural protein not in xenbase Keratin type II Keratin type II tropicalis pBS SK(-) Amp

HAR-248 khdrbs1 Sam68 RNA binding n/a sam68 tropicalis CS107 amp

90 kit serine/threonine kinase n/a c-kit laevis MOD C.Baker ? Amp

HAR-256 kit ser/thr kinase receptor n/a kit krk1 xkrk-1 xkrk1, c-kit tropicalis pCMV-SPO amp BQ389278

HAR-253 kitlg KIT ligand signalling molecule n/a kitlg steel-2 steel2 stem cell tropicalis pExpress amp CF590621

853 klf2 lklf vascular TF Klf2 IMAGE:6640768 no pictures p tropicalis pCMV-SPORT6 Amp Image EST 6640768

HAR-270 n/a structural protein n/a kr t10 cytokeratin, epiderma tropicalis pCMV-SPO amp

iroquois homeobox 4

XB-CLONE-14574190


bug is resolved

iroquois homeobox 5

XB-CLONE-14574193


bug is resolved

ISL LIM homeobox 1

islet-1, Xisl-1, Xislet-1, islet1

XB-CLONE-12441339

XB-IMG-48613; XB-IMG-48614; XB-IMG-48615;

XB-IMG-48616

ISL LIM homeobox 1


XB-CLONE-4240274

trops unspecified stage/ 15/28

ISL LIM homeobox 1



XB-CLONE-2975031

ISL LIM homeobox 1


XB-CLONE-14574240

ISL LIM homeobox 1


XB-CLONE-12442064

ism1 [Predicted: Source is

Human Entrez Gene]

isthmin 1, angiogenesis

inhibitor [Predicted: Source is Human Entrez

Gene]

X-Serrate-1, serrate-1, serrate, jagged1

signal transduction / notch signaling

pathwayXB-CLONE-2175777

transcriptional activator

XB-CLONE-2055935

Image EST 6957041. Accession BC074377. Also known as AP1

kcnh2 [Predicted: Source is

Human Entrez Gene]

potassium voltage-gated channel,

subfamily H (eag-related), member 2 [Predicted: Source is Human Entrez

Gene]

erg1, herg1, herg, lqt2, sqt1, kv11.1, k(v)11.1

K+-channel ERG and related proteins,

contain PAS/PAC sensor domain

XB-CLONE-12441300

kdr [Predicted: Source is

Human Entrez Gene]

kinase insert domain receptor (a

type III receptor tyrosine kinase)


Gene]

flk1, flk-1, vegfr-2, vegfr2, xflk-1

keratin type II not in xenbase

KH domain containing, RNA binding, signal transduction associated 1

RNA-binding KH domain /

v-kit Hardy-Zuckerman 4 feline

sarcoma viral oncogene homolog

c-kit, krk1, pbt, scfr, xkl-1

v-kit Hardy-Zuckerman 4 feline

sarcoma viral oncogene homolog

c-kit, krk1, pbt, scfr, xkl-1

Xsl, Xsl-1, Xsl-2, Xkl-1, steel

Kruppel-like factor 2 (lung)

XB-CLONE-2164957

krt10 [Predicted: Source is

Human Entrez Gene]

keratin 10 [Predicted: Source is Human Entrez

Gene]










have to check them-Maya Piccinni

Have to upload them.-Maya Piccinni

I437

have to check them -Maya Piccinni

I443

Have to upload them. -Maya Piccinni

750 cytoskeletal protein L-plastin IMAGE:4032738 yes p laevis pCMV-Sport6 Amp Image EST 4032738. 98% identical to Image 4964030

LJ-14 ldb1 xldb1, nli, clim2 transcription cofactor Xldb1 pSP64 RI Xldb1 pSP64 RI not needed p laevis pSP64 Amp

677 lef1 xlef-1, lef-1, xlef1 pCS2+ΔN-XLEF-1 not needed p laevis Roel et al 2002 PMID: 12445388 pCS2+ Amp lacks N-terminal Bcat binding domain

678 lef1 xlef-1, lef-1, xlef1 XLEF1 pCS2plus-XLEF-1 not needed p laevis Roel et al 2002 PMID: 12445388 pCS2+ Amp

91-SPH lef1 xlef-1, lef-1, xlef1 pXlef-1 pXlef-1 not needed laevis pcDNA3 AMP

HAR-183 lefty TGFb signal modulator Lef ty Tgas031A08 Trops 12-16-25-40 p tropicalis CS107 amp

2-ER lefty TGFb modulator pCS2 Antivin pCS2 Antivin no pictures laevis Int J Dev Bio 49,781-796 pCS2+ Amp Nodal and Activin inhibition

HAR-192 lefty TGFb signal modulator n/a Lefty lefty antivin tropicalis CS107 amp

HAR-451 lfng lunatic fringe LFNG IMAGE:8850313 no pictures p tropicalis CS111 amp

36-ER lfng lunatic fringe not in xenbase Lunatic Fringe pCDNA3-Lunatic Fringe laevis Int J Dev Biol 49, 781-796 Amp Xenopus Lunatic Fringe

95 lhx1 LIM homeobox 1 kidney TF pXH32-3 p p laevis Taira et al pBluescript KS+ Amp

320 lhx1 LIM homeobox 1 Xlim-enR not needed p laevis pGem amp

HAR-234 lhx1 LIM homeobox 1 TF n/a lhx1 lim-1 lim1 tropicalis CS107 amp not full length CX494294

LJ-6 lhx1 LIM homeobox 1 kidney/urogenital TF Xlim1 pSP64T Xlim1 pSP64T not needed p laevis pSP64T Amp

LJ-23 lhx1 LIM homeobox 1 kidney/urogenital TF XlimI pBSKS XlimI pBSKS no pictures p tropicalis pBLUESCRIPT KS Amp

96 lhx3 LIM homeobox 3 LIM-3, xLIM-3, lim3 neural TF pXH23-1 p p laevis Taira PBS KS+ Amp

587 lmo2 LMO-2, xlmo2, xlmo-2 blood TF LMO2 IMAGE:4174203 p laevis pCMV-SPORT6 Amp IMAGE EST 4174203, Accession number AAH97502

776 lmo4.1 Xlmo4 LMO4 IMAGE:4032190 no pictures p laevis pCMV-Sport6 Amp Image EST 4032190

LJ-17 lmx1b.1 Xlmx1b Xlmx1b mut pRN3 Xlmx1b mut pRN3 ? p laevis pRN3 Amp

LJ-15 lmx1b.1 Xlmx1b Xlmx1b pRN3 Xlmx1b pRN3 not needed p laevis pRN3 Amp

926 lrrc10 n/a Leucine rich repeat Lrrc10 Lrrc10 (4409258) no pictures p laevis pCMV-SPORT6 Amp Image EST 4409258, Accession BC084086

805 n/a transcription reguator HRLRR IMAGE:4407794 no pictures p laevis pCMV-Sport6 Amp

107 mad2l1 mad2, XMad2 TGFb dependent TF XMAD2myc XMAD2myc not needed p laevis sp64T? Amp

349 mafb eye TF RT-XMafB p laevis pBluescript SK+ Amp

554 mafb Blood TF XmafB pCS2plus-XmafB not needed p laevis Ishibashi&Yasuda 2001. Mech Dev 101: 155-166 pCS2+ Amp Full length XMafB

562 map3k7 xTAK1, MAPKKK, tak1 ser/thr kinase TAK1 xTAK1-pBSKS- no pictures p laevis Shybuya et al. 1998. EMBO J 17: 1019-1028 pBluescript SK- Amp

lcp1 [Predicted: Source is

Human Entrez Gene]

lymphocyte cytosolic protein 1

(L-plastin) [Predicted: Source is Human Entrez

Gene]

lpl, l-plastin, cp64, pls2, lc64p

XB-CLONE-2112800

LIM domain binding 1

XB-CLONE-11536618

lymphoid enhancer-binding

factor 1 HMG box transcription factor, wnt signaling

XB-CLONE-12441306

pCS2plus-deltaN-XLEF-1



XB-CLONE-12441309



XB-CLONE-14573996


bug is resolved

left-right determination

factor

leftb, leftyb, lefty-1, antivin, atv, Xlefty,

XATVXB-CLONE-4119373


factor


XATVXB-CLONE-8715422

have to check when bug is solved


factor


XATVLFNG O-

fucosylpeptide 3-beta-N-

acetylglucosaminyltransferase

glycosyltransferase, signaling molecule

XB-CLONE-2428026

LFNG O-fucosylpeptide 3-

beta-N-acetylglucosaminyl

transferase

glycosyl transferase + signalling molecule

Xlim1, Xlim-1, Lim1, lim-1, lim1

XB-CLONE-11885379

Note that I had some trouble with this and had to gene clean the plasmid prep before digestion.


LIM and homeodomain containing

transcription factor XB-CLONE-8596615



XB-CLONE-11536589


XB-CLONE-11917784XB-CLONE-6541310

LIM domain only 2 (rhombotin-like 1)

XB-CLONE-2320124

sent 4 pictures to xenbase on 5.04.13

LIM domain only 4, gene 1

Transcription factor, contains HOX domain

XB-CLONE-2110636


XB-CLONE-11536629

The lmx1b gene is pivotal in glomus development in Xenopus laevis.

Dev Biol. October 1, 2008; 322 (1): 74-85.

LIM homeobox transcription factor

1, beta, gene 1 homeodomain


leucine rich repeat containing 10


Gene]

XB-CLONE-2265183

lrrc10 [Predicted: Source is

Human Entrez Gene]

leucine rich repeat containing 10


Gene]

XB-CLONE-2273930

Image EST 4407794. Also known as SOC-2. Identical to Image 4409258, Accession BC084086

MAD2 mitotic arrest deficient-like

1 [Predicted: Source is Human

Entrez Gene]

XB-CLONE-12271639

v-maf avian musculoaponeuroti

c fibrosarcoma oncogene homolog

B [provisional]

kreisler, xmafb, valentino

XB-CLONE-12440862

XB-IMG-48643XB-IMG-48644XB-IMG-48645XB-IMG-78436XB-IMG-78435

v-maf avian musculoaponeuroti

c fibrosarcoma oncogene homolog

B [provisional]

kreisler, xmafb, valentino

XB-CLONE-12441240

mitogen-activated protein kinase kinase kinase 7

XB-CLONE-12441259












Have to upload the pictures-Maya Piccinni

in xenbase pGEM-72f- vector, in database pGem-Maya Piccinni


I454

Have to upload the pictures -Maya Piccinni

A465

in xenbase pGEM-72f- vector, in database pGem -Maya Piccinni

F474


564 map3k7 xTAK1, MAPKKK, tak1 ser/thr kinase TAK1 KN xTAK1KN/pCS2+ not needed p laevis Shybuya et al. 1998. EMBO J 17: 1019-1028 pCS2+ Amp

464-AP TF Xmax pCS2MT laevis pCS2MT Amp 4095bp

HAR-325 mdm2 Xdm2 E3 ubiquitin ligase n/a mdm-2 mdm-2 mdm2 tropicalis pCMV-SPO amp Full length BC063898

861 mef2a somite TF Mef2a IMAGE:5536990 have to upload them p laevis pCMV-SPORT6 Amp Image EST 5536990, Accession BC046368

NP-E meis2 Meis homeobox 2 mrg1 MEIS2 TTpA048a05 no pictures tropicalis pCS107 Amp TEpA048a05

HAR-25 meis3 Meis homeobox 3 XMeis3, mrg2 TF MEIS3 L1G9f05 p tropicalis CS107 amp MEIS3 Meis3 meis3 - not full length - AL637544

HAR-26 meis3 Meis homeobox 3 XMeis3, mrg2 TF Meis3 TGas014k23 n/a p tropicalis CS107 amp MEIS3 Meis meis3 meis - not full length - AL632424

HAR-27 meis3 Meis homeobox 3 XMeis3, mrg2 TF Meis3 TNeu006o21 n/a p tropicalis CS107 amp AL640600 - Uncertain length -Tneu006021

789 Mesoderm TF Thylacine1 IMAGE:4058881 no pictures p laevis pCS2+ Amp Image EST 4058881

369 mix1 mesoderm TF pBSRN3Mix1 not needed p laevis Lemaire et al. Development (1998) Vol125 pp 2371-2380 pBluescript SK+ Amp

319 mix1-a endodermal Tf XBm 3m not needed p laevis XBm amp

HAR-313 mixer tMixer TF n/a Mixer mixer tropicalis CS107 amp AL965856

298 mixer tMixer endodermal TF Mixer not needed p laevis pCS300 Amp

HAR-420 mks1 n/a MKS1-short TGas092g16 no pics p tropicalis CS107 amp Full length - CR942566

HAR-421 mks1 n/a MKS1-long IMAGE:7822235 n/a tropicalis CS107 amp Full length BC123067.1 7822235

851 mmp9 metalloprotease MMP9 IMAGE:4965074 have to upload them p laevis pCMV-SPORT6 Amp Image EST 4965074

19-NP TF HB9 HB9 no pictures p laevis Blue Script KS Amp

1050-AP TF xHB9 pBSK xHB9 no pictures p laevis pBSK- Amp

HAR-457 msc musculin n/a transcription factor myoR IMAGE:7603626 no pictures p tropicalis CS108 amp Full length. Accession number BC135361.

594 msx1 msh homeobox 1 xMsx1-pSP64T XB not needed p laevis Suzuki et al 1997. Dev 124: 3037-3044 pSP64T XB Amp

106 mxd1 Mad1, xmad1 TF dimerisation XMAD1 SP64TENXMAD1 not needed p laevis SP64TEN Amp

555 myb c-myb, xmyb, cmyb TF XcMyb c-MybRN3 no pictures p laevis Amaravadi&King, 1994. Oncogene 9: 971-974 pRN3 Amp Does not contain any 5' or 3' UTR

871 mybpc3 n/a CMBPC IMAGE:4408774 no pictures p laevis pCMV-SPORT6 Amp Image EST 4408774. Cardiac myosin binding protein C

HAR-128 myf5 myogenic factor 5 Xmyf5, Xmyf-5, myf-5 muscle TF Myf-5 TGas046o13 n/a p tropicalis CS107 amp

HAR-132 myf5 myogenic factor 5 Xmyf5, Xmyf-5, myf-5 muscle TF myf5 TGas042g05 p tropicalis CS107 amp 5' present. Accession number AL654739.

864 myf5 myogenic factor 5 Xmyf5, Xmyf-5, myf-5 Myf5 IMAGE:3517121 no pictures p laevis pCS2+ Amp Image EST 3517121

AP-9 myf5 myogenic factor 5 Xmyf5, Xmyf-5, myf-5 TF Xmyf 5 Xmyf 5 pSP64T not needed p laevis psp64t Amp

11 MYOD n/a MYOD MYOD(M3) laevis

HAR-5 myod1 somite TF myod1 TNeu010f07 no pics p tropicalis CS107 amp Myod myod - 3' present - AL638062 - Tneu010F7

HAR-8 myod1 somite TF myod1 TNeu142k23 no pics p tropicalis CS107 amp Myod myod - 5' present - AL795473 - Tneu142K23

HAR-9 myod1 somite TF myod1 TNeu016c22 no pics p tropicalis CS107 amp Myod myod - full length - AL642930 - Tneu016C22

HAR-10 myod1 somite TF myod1 TNeu017h11 trops14-22-23-28 p tropicalis CS107 amp

HAR-15 myod1 somite TF myod1 TNeu057j16 no pics p tropicalis CS107 amp Myod myod - not full length - AL677695

mitogen-activated protein kinase kinase kinase 7

XB-CLONE-12441267

max [provisional]

MYC associated factor X

[provisional]

bhlhd4, bhlhd5, bhlhd6, bhlhd7,

bhlhd8, xmax, xmax2, xmax4

Mdm2, p53 E3 ubiquitin protein ligase homolog

(mouse)

myocyte enhancer factor 2A

xmef2a, adcad1, rsrfc4, rsrfc9, sl2, sl-2

XB-CLONE-2203532


XB-CLONE-4182840

XB-CLONE-4453828

trops 13-19-20-23-29-35

XB-CLONE-4134274

XB-CLONE-4337501

mespa [provisional]

mesoderm posterior homolog

A [provisional]

thyl2, thylacine 2, thylacine2, hen2, thyl1, thylacine 1, thylacine1

XB-CLONE-2327051

Mix paired-like homeobox

mixl1, mix.1, mix.2, mix2, tMix

XB-CLONE-12440903

Mix paired-like homeobox

mixl1, mix.1, mix.2, mix2, tMix

XB-CLONE-12271735

mix-like endodermal

regulator mix-like

endodermal regulator

XB-CLONE-12271626

to amplify pcr fragment use oligos major 68 and major 69

Meckel syndrome, type 1


XB-CLONE-3994292

Meckel syndrome, type 1


XB-CLONE-3423625

matrix metallopeptidase 9

(gelatinase B, 92kDa gelatinase,

92kDa type IV collagenase)

MMP-9, gelb, clg4b, mandp2

XB-CLONE-2041688

mnx1 [provisional]

motor neuron and pancreas

homeobox 1 [provisional]

hlxb9, hb9, scra1, hoxhb9

XB-CLONE-14575104

mnx1 [provisional]

motor neuron and pancreas


hlxb9, hb9, scra1, hoxhb9

XB-CLONE-12442067

XB-CLONE-3038201

hox7, hox-7, msx-1, Xmsx-1, xmsx1, Xhox-

7.1homeodomain


MAX dimerization protein 1

XB-CLONE-11920576

XMAD1, Xmad1, mad1, MAD1, smad1, SMAD1, Xsmad1, XSMAD1

v-myb avian myeloblastosis viral oncogene

homolog

XB-CLONE-12441243

myosin binding protein C, cardiac

Projectin/twitchin and related proteins

XB-CLONE-2265512

XB-CLONE-4125732

TGas046013 This was supposed to be flk-1. Sequencing and in situ suggest that it is xMyf-5 - AL651980

XB-CLONE-4121370

trops unspecified stage x2/12/13

myogenic helix-loop-helix transcription


XB-CLONE-14575017

myogenic differentiation 1

[provisional]

myogenic factor 3, MyoD, XtmyoD, XMyoD, mf25

myogenic helix-loop-helix transcription

factor myogenic

differentiation 1 [provisional]


XB-CLONE-4348299


[provisional]


XB-CLONE-4284358


[provisional]


XB-CLONE-4347347


[provisional]


XB-CLONE-4331833

Myod myod - full length - AL645144 - Tneu017H11RI/BamHI cut inside clone



[provisional]

myogenic factor 3, MyoD, XtmyoD,

XMyoD,mf25XB-CLONE-4312813













Vector xBm?-Maya Piccinni

A490

Vector xBm? -Maya Piccinni

59-KL muscle TF p3 MyoD full length no pictures p laevis pBSKs+ Amp MyoD

AP-72 muscle TF XmyoD not needed p laevis psp64t Amp

749 nap1l1 nap1, xnap1l, xnap1l1 chromatin assembly NAP1 NAP1-pBluescript no pictures p laevis Steer et al 2003. Mech Dev 120: 1045-1057 pBluescript Amp

15-ER nbl1 DAN, no3, dand1 not in xenbase Dan pCS2-DAN laevis Int J Dev Bio 49,781-796 pCS2+ Amp Xenopus DAN seorted inhibitors

33-NP ncam1 ncam, n-cam cell adhesion N-Cam N-Cam no pictures p laevis Amp

3 ncam1 ncam, n-cam cell adhesion n/a NCAM PN1 no laevis pSP72, 2.9Kb Amp

877 ncf2 n/a Ncf2 IMAGE:7008637 yes p laevis pCVM-SPORT6 Amp

878 n/a NADPH oxidase Ncf4 IMAGE:5513002 yes p laevis pCMV-SPORT6 Amp

HAR-252 ndrg1 TF n/a NDGR1 ndrg1 tropicalis pCMV-SPO amp CF238475

HAR-267 ndrg1 intracellular signalling n/a NDRG1 tropicalis pRKW2 amp CF783804

HAR-293 nes nestin tanabin, nestin cytoskeletal n/a Tanabin tanabin nestin tannabin tropicalis pCMVspor amp CX897063

77-KL neurod1 MTx12A CS2plus-MTx12A not needed p pCS2+ Amp Full length Xenopus neuroD cDNA (1.4 Kb)

HAR-285 neurod1 TF n/a neurod1 neuroD tropicalis pCMV-Spor amp 5' present CF149433

HAR-286 neurod1 TF n/a neurog1 neurogenin-1 tropicalis pCMV-Spor amp 5' present CF224521

632-AP neurod1 neural TF not in xenbase NeuroD Xenopus NeuroD laevis pCS2+rep Amp

32-NP neurog2 neurogenin 2 TF XNgnr-1 XNgnr-1 no pictures p tropicalis Amp

HAR-284 neurog2 neurogenin 2 TF n/a neurogenin-re neurogenin-2 tropicalis CS107 amp CK655816

856 nf2 merlin NF2 IMAGE:5156434 no pictures p laevis pCMV-SPORT6 Amp Image EST 5156434, Accession BC077822

897 nfe2l2 NF-E2R2 NF-E2R2 IMAGE:5571056 have to upload them p laevis pCMV-SPORT6 Amp Image 5571056. Accession BC043997

HW-34 nfkb1 transcription modulator n/a pJC2L IκB pJC2L IκB laevis pJC2L amp IB in pJC2L.

HAR-315 nfkb1 transcription modulator n/a Ikb ikappab, NF-kappaB tropicalis CS107 amp Full length AL786990

HAR-228 nkx2-1 NK2 homeobox 1 TF n/a ttf1 Nkx2.1, thyroid tropicalis PCRII-TOPO amp DN081200 - not full length

902 nkx2-3 NK2 homeobox 3 NKX2.3b2 IMAGE:7979170 have to upload them p laevis pExpress1 Amp Image EST 7979170

224 nkx2-5 NK2 homeobox 5 Xnkx2.5 Xnkx2 5 p laevis pGEM3z Amp

HAR-113 nkx2-5 NK2 homeobox 5 Heart TF Nkx2-5 nkx2.5 n/a p tropicalis pCR2.1-topo 3.9 kb amp DN008946 - Not full Length

HAR-354 nkx2-5 NK2 homeobox 5 n/a Nkx2.5 Nkx2.5 , tinman tropicalis CS107 amp Full length DN012877

myod1 [provisional]


[provisional]


XB-CLONE-12442455

myod1 [provisional]


[provisional]


XB-CLONE-12271883

XmyoD plus 100bp 3prime UTR

nucleosome assembly protein

1-like 1 XB-CLONE-12441523

neuroblastoma 1, DAN family BMP

antagonist BMP signalling

modulator

neural cell adhesion molecule

1XB-CLONE-14575110

neural cell adhesion molecule

1 Kintner and Melton(1997) Development Chris

Kintner, Salk Inst (601) 485 6675

neutrophil cytosolic factor 2

NADPH oxidase complex component

XB-CLONE-2028258

Image EST 7008637. Accession BC074140. Also known as p67phox

ncf4 [Predicted: Source is

Human Entrez Gene]

neutrophil cytosolic factor 4, 40kDa


Gene]

XB-CLONE-2046818

Image EST5513002. Accession BC103734. also known as p40phox

N-myc downstream regulated 1

xndrg1, gc4, rtp, drg1, ndr1, nmsl, tdd5,

cap43, cmt4d, hmsnl, rit42, targ1, proxy1

N-myc downstream regulated 1

gc4, rtp, drg1, ndr1, nmsl, tdd5, cap43,

cmt4d, hmsnl, rit42, targ1, proxy1

neuronal differentiation 1

XNeuroD, neurod, neuroD

helix-loop-helix transcription factor

XB-CLONE-12442478

Xenopus laevis neuronal differentiation

1 (neurod1-a)







X-ngnr-1, neurogenin-related-1, Ngnr1,

XNgnr1, ngn2, atoh4, Ngnr-1, neurogenin-2, ngn-2, Xngnr-1, xngn2,

neurogenin 2

XB-CLONE-14575107

neurogenin-related-1, Ngnr1, XNgnr1, ngn2,

atoh4, Ngnr-1, neurogenin-2, ngn-2,

Xngnr-1, xngn2, neurogenin 2

neurofibromin 2 (merlin)

cytoskeleton component

XB-CLONE-2148539

nuclear factor (erythroid-derived

2)-like 2



nuclear factor of kappa light

polypeptide gene enhancer in B-cells

1

kbf1, ebp-1, nfkb-p50, nf-kappaB, nfkb-p105,

nf-kappa-b

See: Richardson, J. C., Garcia Estrabot, A. M. and Woodland, H. R. (1994). XrelA, a Xenopus maternal and zygotic homologue of the p65 subunit of NF-kappa B. Characterisation of transcriptional properties in the developing embryo and identification of a negative interference mutant. Mech Dev 45, 173-89.Richardson, J. C., Gatherer, D. and Woodland, H. R. (1995). Developmental effects of over-expression of normal and mutated forms of a Xenopus NF-B homolog. Mechanisms of Development 52, 165-177.EStrabot, A. G. PhD thesis Warwick University.

nuclear factor of kappa light

polypeptide gene enhancer in B-cells

1

kbf1, ebp-1, nfkb-p50, nf-kappaB, nfkb-p105,

nf-kappa-b

ttf1, nkx2.1, titf1, Xnkx-2.1, xnkx2-1 xnkx-2.3, nkx-2.3, nkx2.3, xnkx2-3


XB-CLONE-1652162

tinman, csx, XNkx-2.5, Nkx-2.5, nkx2.5,

XNkx2-5, nkx-2.5, AR2

homeodomain transcription factor with role in cardiac

development

XB-CLONE-12062492

XB-IMG-78384XB-IMG-78385XB-IMG-78386XB-IMG-78387

tinman, csx, XNkx-2.5, Nkx-2.5, nkx2.5, XNkx2-5, nkx-2.5

XB-CLONE-14575518


homeodomain transcription factor with role in cardiac

development













Have to upload them-Maya Piccinni


I519


I520

Have to upload them -Maya Piccinni

F527


437-AP nkx2-5 NK2 homeobox 5 Nkx2.5 Xenopus tinman no pictures p laevis pGEM3z Amp

911 nkx2-6 nkx2.6, Xnkx2-10, tix NKX2.10 NKX2.10 (7517841) no pictures p tropicalis pCS22+ Amp Image EST 7517841

HAR-143 nkx2-8 NK2 homeobox 8 mgc75807, nkx2.8 TF Nkx TNeu010a20 n/a p tropicalis CS107 amp Accession number AL638126 - Tneu010A20

910 nkx3-1 Xbap, nkx3.1, koza NKX3.1 NKX3.1 (5537411) p laevis pCMV-SPORT6 Amp Image EST 5537411, Accession BC047968

HAR-127 nkx6-1 NK6 homeobox 1 nkx6.1 TF nkx6-1 TEgg079m07 n/a p tropicalis CS107 amp Tegg079m07 - not full length

HAR-356 nkx6-2 NK6 homeobox 2 nkx6.2 n/a Nkx6.2 nkx6.2 tropicalis CS107 amp not full length AL638236

HW-41 nodal signalling molecule pSP-Xnr4 pSP-Xnr4 laevis

HAR-22 nodal signalling molecule XNR3 TGas011k18 n/a p tropicalis CS107 amp xnr3 XRN3 x-nr3 - Full Length - AL629006

362 nodal1 Xnr1 n/a p laevis pBS SK Amp

HW-40 nodal1 pSP-Xnr1 pSP-Xnr1 laevis

363 nodal2 Xnr-2, Xnr2, nr2 Xnr2 n/a p laevis pBS SK Amp

364 nodal3.1 Xnr3, nr3, Xnr-3 Xnr-3 have to re do them p laevis From Mike Jones lab pGEM5Zf Amp

HAR-53 Xnr3, nr3, Xnr-3 Fugacin IMAGE:4462861 n/a p tropicalis CS107 amp

HAR-311 Xnr3, nr3, Xnr-3 signalling molecule n/a Xnr3/EcorV- Xnr3delta EcorV, Xnr3 tropicalis CS107 amp AL629006

46-ER Xnr3, nr3, Xnr-3 signalling molecule not in xenbase Xnr3 pCS2+ xNR3 laevis Int J Dev Biol 49, 781-796 pCS2+ Amp Xnr3 full length

339 nodal5.2 pBS-Xnr5 n/a p laevis Takahashi et al., 2000 Development 127, 5319-5329 pBS AMP

340 nodal6 xnr6b, nr6, xnr6 pBS-Xnr6 p laevis Takahashi et al., 2000 Development 127, 5319-5329 pBluescript SK (-)

HAR-140 nog noggin n/a nog1 noggin nog tropicalis CS108 amp AY672091

HAR-334 nog noggin n/a Xtnog1-pBSK tropicalis CS107 amp AY672091

HAR-335 nog noggin n/a Xtnog1-108 tropicalis CS107 amp AY672091 Full Length

5-CC nog noggin TGFb modulator Noggin Noggin not needed p laevis pCS105 Amp Extracellular Regulators

38_ER nog noggin xNoggin pCDNA3xNoggin laevis Int J Dev Biol 49, 781-796 pCDNA3 Amp xNoggin Full length

HAR-333 noggin 2, noggin-2 n/a Xtnog2-2-107 tropicalis CS107 amp AY672092

64 not xnot Xnot10 no p laevis BS KS+ amp

HAR-118 not notocord TF XNOT TNeu017e01 trops 18/20/21/28 p tropicalis CS107 amp

441-AP notch1 notch 1 notch signalling not in xenbase Xnotch ICD Xenopus Notch ICD laevis pCS2+ Amp

781 notch1 notch 1 Notch1 IMAGE:4680203 no pictures p laevis pCMV-Sport6 Amp Image EST 4680204

916 notch1 notch 1 Notch1a IMAGE:8322195 have to upload them p laevis pExpress1 Amp Image EST 8322195.

892 casil, cadasil signalling molecule Notch4 IMAGE:4684242 no pictures p pCMV-SPORT6 Amp Image EST 4684242



XB-CLONE-12442140

NK2 homeobox 6 [provisional]


XB-CLONE-3677463

XB-CLONE-4340553

NK3 homeobox 1 [provisional]


XB-CLONE-2201992

XB-IMG-78813XB-IMG-78814XB-IMG-78815XB-IMG-78816

XB-CLONE-4053686


nodal homolog 1 [provisional]

nr1, Xnr-1, Xnr1, nodal-related 1,

induction of axial mesoderm, nr1-a

XB-CLONE-14571773

UNSPECIFIED (1999) Mode of action of VegT in mesoderm and endoderm formation. DEVELOPMENT, 126 (21). pp. 4903-4911. ISSN 0950-1991

nodal growth differentiation

factor

Xnr-4, Xnr4, nr4, nodal4, Nodal homolog

4XB-CLONE-4275580





XB-CLONE-12440881

From Mike Jones Lab Nodal-related signals induce

axial mesoderm and dorsalize mesoderm during gastrulation.

Jones CM, Kuehn MR, Hogan BL, Smith JC, Wright CV.Development. November 1, 1995; 121 (11): 3651-62.




transforming growth factor beta and bone

morphogenetic protein related protein

XB-CLONE-14571771

UNSPECIFIED (1999) Mode of action of VegT in mesoderm and endoderm formation. DEVELOPMENT, 126 (21). pp. 4903-4911. ISSN 0950-1991



XB-CLONE-13586331

nodal homolog 3, gene 1

[provisional] TGFb signalling

moleculeXB-CLONE-12440888

nodal3.1 [provisional]


[provisional]

transforming growth factor beta and bone


XB-CLONE-4456959

NOT FULL LENGTGH - see Genbank ID# BG886280dad51c07.x1 Wellcome CRC pCS107 tropicalis St10-12

Silurana tropicalis cDNA clone IMAGE:4462861 3' similar to TR:Q91621 Q91621 FUGACIN. ;, MRNA sequence

gi|14263370|gb|BG886280.1|[14263370]



[provisional]


nodal homolog 3, gene 1 [provisional


[provisional] xnr5, nr5, Xnr5-14,

nodal5 TGFb signalling

moleculeXB-CLONE-12271680



XB-CLONE-12271740

XB-IMG-48373 only one not very good, need early stages

nog-A, noggin 1, noggin, nog1, noggin-1

TGFb signalling modulator


extracellular BMP antagonist


extracellular BMP antagonist


XB-CLONE-12442168



nog2 [provisional]

noggin 2 [provisional]

extracellular TGFb modulator

notochord homeobox

not, noto, Xnot-2, Xnot2


XB-CLONE-11536568

notochord homeobox

Xnot, noto, Xnot-2, Xnot2

XB-CLONE-4333489

AL643117 - 5' present -

3' end not present...maybe not be xnottneu017e01

notch, xotch, xnotch, notch-1, xnotch1


signal transduction, receptor for delta class

ligandsXB-CLONE-2249444



ligandsXB-CLONE-1605540

notch3 [Predicted: Source is

Human Entrez Gene]

notch 3 [Predicted: Source is Human

Entrez Gene]XB-CLONE-2196168










Have to upload them-Maya Piccinni


Add as synonim http://www.ncbi.nlm.nih.gov/gene/4855-Maya Piccinni

G567

Add as synonim http://www.ncbi.nlm.nih.gov/gene/4855 -Maya Piccinni

862 nphs1 nephrin signalling molecule Nephrin IMAGE:4964461 have to upload them p laevis pCMV-SPORT6 Amp Image EST 4964461, Accession AY902238

HAR-417 nr1h5 for1, for2 hormone receptor For1 IMAGE:8901234 no pics p tropicalis CS107 amp 8901234

473-SPH nr2e1 Tll, xTLL, tailless, tlx hormone receptor Xtailless (Tll) Xtailless-Tll no pictures p laevis pCS2+ AMP Accession Number U67886.

590 nrp1 neuropilin 1 Nrp-1 IMAGE:4969039 p laevis pCMV-SPORT6 Amp IMAGE EST 4969039

HAR-62 nrp1 neuropilin 1 receptor nrp1 trops 19/22/24/28 tropicalis CS107 amp

734 ntrk2 trkb, xtrkb NGF receptor xTrkB IMAGE:6944839 have to upload them p laevis pCMV-SPORT6 Amp Image EST 6944839

237-NP numb numb homolog S171 cell polarity regulation Xnumb BSK Xnumb BSK tropicalis BKS Amp

HAR-452 pla2l, otoconin 90 phospholipase Oc90 /CS108 IMAGE:7626568 no pictures p tropicalis CS108 amp

HAR-208 olfm1 olfactomedin 1 noelin-1 secreted glycoprotein Noelin TNeu071d23 n/a p tropicalis CS107 amp Accession number AL780483

HAR-299 olig1 n/a TF n/a Olig1 olig1 olig-1 no pics tropicalis pCMV SPORT6 amp cx852777

HAR-463 olig1 n/a transcription factor not in xenbase Olig1 erg, vascular ets factor, tropicalis

HAR-124 olig3 n/a oligodendrocyte TF olig3 TGas060e07 p tropicalis CS107 amp

HAR-130 mgc89336 oligodendrocyte TF olig4 TNeu076f16 n/a p tropicalis CS107 amp

HAR-133 mgc89336 oligodendrocyte TF olig4 TNeu063g03 trops 15/17/23/32 p tropicalis CS107 amp Accession number AL777479

HAR-312 osr1 odd1 not known n/a Osr1 osr-1 osr1 odd skipped tropicalis CS107 amp CR761835

NP-B ostf1 sh3d3 OSTF1 TEgg069m06 no pictures tropicalis pCS107 Amp TEgg069m06

HAR-448 otx1 xotx1 Otx1 IMAGE:5379560 no pictures p tropicalis pCMV-SPORT6.1 amp

HAR-29 otx2 Xotx2, otx-2, Xotx-2 TF OTX2 TGas025f18 n/a p tropicalis CS107 amp

HAR-31 otx2 Xotx2, otx-2, Xotx-2 TF OTX2 L1G12g10 trops 13/18/20/32 p tropicalis CS107 amp

181-SPH otx2 Xotx2, otx-2, Xotx-2 not in xenbase pXOT30.1 pXOT30-1 laevis

429-AP pak3 ser/thr kinase not in xenbase Xpak3 Xpak3-cDNA laevis pBK-CMV Amp AF485330

232-NP pard6b Par-6, par6 cell polarity regulation par6 pBS par6 pBS laevis pBS Amp

HAR-261 pax1 paired box 1 pax-1 TF n/a Pax1 pax-1 pax1 tropicalis CS107 amp BX723020

HAR-114 pax2 paired box 2 XPax2, XPax-2, pax-2 TF Pax2 TNeu062i10 n/a p tropicalis CS107 amp

LJ-5 pax2 paired box 2 XPax2, XPax-2, pax-2 kidney/eye TF X Pax-2 64TS X Pax-2 64TS not needed p laevis pSP64T Amp

1102-AP pax2 paired box 2 XPax2, XPax-2, pax-2 TF Pax2 Pax2-pBSKplus no pictures p laevis pBSK2+

HAR-79 pax3 paired box 3 TF PAX3 TNeu031e04 trops 13/16/19/23/32 p tropicalis CS107 amp Tneu031e04 - AL656612

HAR-129 pax3 paired box 3 neural TF pax3 TNeu048l09 n/a p tropicalis CS107 amp AL658809 - Full length

863 pax3 paired box 3 neural TF Pax3 IMAGE:7981250 no pictures p laevis pExpress1 Amp Image EST 7981250, Accession BC108573

1098-AP pax3 paired box 3 TF pax3 pax3 laevis

473 pax5 paired box 5 Pax-5 testis TF Pax5 pCS-5Pax16-4 not needed p laevis Heller and Brandli (1999) Dev Genet 24: 208-219 pCS2+ Amp

HAR-182 pax6 paired box 6 TF Pax6 IMAGE:6992220 Trops 13-15-18-33/34 p tropicalis pCMV-sport6.1 amp

nephrosis 1, congenital, Finnish

type (nephrin)XB-CLONE-2228962

nuclear receptor subfamily 1, group

H, member 5XB-CLONE-2599174

nuclear receptor subfamily 2, group

E, member 1 XB-CLONE-14574222

A5 antigen, np-1, neuropilin-1, nrp, npn-

1, nrp-1, neuropilintransmembrane

receptorXB-CLONE-2206239


resolved

A5 antigen, np-1, neuropilin-1, nrp, npn-

1, nrp-1, neuropilin

not sure of how to put it

accession number does

not correspond to gene

NRP1 nrp-1 neuropilin 1 - Genbank #BG512402 - not full length


neurotrophic tyrosine kinase, receptor, type 2

XB-CLONE-2078698

oc90 [Predicted: Source is

Human Entrez Gene]

otoconin 90 [Predicted: Source is Human Entrez

Gene]

XB-CLONE-2967508

XB-CLONE-4301346

oligodendrocyte transcription factor

1 oligodendrocyte

transcription factor 1


3XB-CLONE-4154079

trops 13/23/ unaidentified

not full length - no 5' - 3' uncertainTGas060e07

olig4 [provisional]



Tneu076F16 - AL776242

This clone pulls up chick olig2 as the first hit with tblastx. The next hit is human olig3.

olig4 [provisional]



odd-skipped related transciption

factor 1

osteoclast stimulating factor 1

Microtubule-associated anchor protein involved in

autophagy and membrane trafficking

XB-CLONE-3966229

orthodenticle homeobox 1


XB-CLONE-4430580


XB-CLONE-4144262

OTX2 otx2 otx Otx2 - 5' present - Al629928 - Tgas025F18


XB-CLONE-4452878

otx2 Otx2 OTX2 otx - 5' present - LIG12G10 - AL643588*this clone has been tested and works for in situ



p21 protein (Cdc42/Rac)-

activated kinase 3

XPak3, bpak, mrx30, mrx47, ophn3, cdkn1a,

pak3beta

par-6 family cell polarity regulator

beta

XB-CLONE-4260109

CR760264 - BamHI digest/T7 works for in situ. However BamHI digest does produce a second small band 600bp.

XB-CLONE-11536586XB-CLONE-12442049

pax-3, Waardenburg syndrome 1, xpax3

XB-CLONE-4328287


XB-CLONE-4346543


XB-CLONE-1653083


XB-CLONE-12441192

XLPAX6, xpax6, pax-6, an2, mgda, wagr

XB-CLONE-4378887

AGENCOURT_15156619 NICHD_XGC_Emb6 Silurana tropicalis cDNA clone IMAGE:6992220 5', MRNA

sequence gi|33604574|gb|CF264195.1|[33604574]











HAR-465 pax6 paired box 6 n/a Pax 6 tropicalis

1048-AP pax6 paired box 6 TF XPax6 XPax6 laevis pCS2+ Amp

475 pax8 paired box 8 XPax8, XPax-8, pax-8 kidney TF Pax8 pBS.KLPax8.1 p tropicalis Heller and Brandli (1999) Dev Genet 24: 208-219 pBluescript SK- Amp partial Pax8 cDNA isolated form Xenopus adult kidney

HAR-131 pax8 paired box 8 XPax8, XPax-8, pax-8 TF Pax8 IMAGE:7863442 n/a p tropicalis pBS KS II amp

HAR-214 pax8 paired box 8 XPax8, XPax-8, pax-8 TF Pax8 IMAGE:6977138 trops 15/21 p tropicalis CS107 amp

HAR-137 pcdh1 protocadherin 1 cell adhesion n/a Axial tropicalis CS107 amp AL880435 - 5' present

HAR-139 pcdh1 protocadherin 1 cell adhesion n/a Axial apc axial protocadherin tropicalis CS107 amp not full length AL893330

37-NP pcdh7 protocadherin 7 nfpc cell adhesion NF protocadherin NF protocadherin no pictures p laevis PCS2 Amp

757 n/a DNA synthesis Pcna-A IMAGE:5049027 no pictures p laevis pCMV-Sport6 Amp Image EST 5049027, Accesssion BC05778

HAR-414 pdi, XPDIp XPDIP IMAGE:7883256 no pics p tropicalis CS107 amp Full length - DT448626 - 7883256

27-ER pdx1 ipf1, XlHbox8, pdx-1 not in xenbase Xlhbox8 Xlhbox8/pSK laevis Development 104,787-794 pSK Amp

709-AP pdx1 ipf1, XlHbox8, pdx-1 TF XLHBox8 XLHBox8pCS2 laevis pCS2+ Amp

787 pecam1 cd31, pecam-1, pecam haemaglobin alpha CD31 IMAGE:4173797 no pictures p laevis pCMV-Sport6 Amp Image EST 4173797

HW-24 pgat xpat Germplasm formation n/a pBS Xpat Partial pBS Xpat Partial laevis pBluescript amp

HW-26 pgat xpat germplasm formation n/a pJC2LXG1 pJC2LXG1 laevis pSPJC2L amp

HW-14 pgat xpat germ plasm formation n/a pJC xpat pJC xpat laevis

807-AP pin1 XpunI XpunI-pCS2plus-rep not needed p laevis Wheeler GN, Hamilton FS, Hoppler S. Amp

930 pls3 plastin 3 t-plastin Plastin3T Plastin3T (4033072) no pictures p laevis pCMV-SPORT6 Amp Image EST 4033072, Accession BC061655


paired box transcription factor with homeodomain,

involved in eye development


XB-CLONE-12441199

XB-IMG-78743XB-IMG-78744XB-IMG-78745XB-IMG-78746XB-IMG-78747XB-IMG-78748XB-IMG-78749XB-IMG-78750XB-IMG-78751XB-IMG-78752XB-IMG-78753XB-IMG-78754

XB-IMG-78755 all in laevis

XB-CLONE-3152581

DT412463 - approximately 950bp of x.trop pax8 was amplified using the following

primers: ggaattccGGTCACGGAGGTTTGAATCAGTTAG cgggatcccgTGGGGCTACATAAAGATGAAGGG

which also places an EcoRI site at the 5'end and a BamHI site

at the 3'end. This was amplfiied using Pfu from 25-30 stage x.trop

cDNA Amplicon was cloned into the RI-BamHI site of pBS KS

II.

XB-CLONE-4394290




axial protocadherin, axpc

axpc axial protocadherin

axial protocadherin, axpc

XB-CLONE-12442251

pcna [Predicted: Source is

Human Entrez Gene]

proliferating cell nuclear antigen


Gene]

XB-CLONE-2217549

pdia2 [Predicted: Source is

Human Entrez Gene]

protein disulfide isomerase family

A, member 2 [Predicted: Source is Human Entrez

Gene]

Protein disulfide isomerase (prolyl 4-

hydroxylase beta subunit)

XB-CLONE-3087623

pancreatic and duodenal

homeobox 1 homeodomain

transcription factor

pancreatic and duodenal

homeobox 1 platelet/endothelial

cell adhesion molecule 1

XB-CLONE-2320474

Primordial germ cell-associated

transcript protein

Hudson, C., Woodland, H. R., 1998. Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis. Mechanisms of Development. 73,

159-168.

The original clone (F) containing most of the 3’UTR in pBluescript. Cloned from Zap library


transcript protein

Machado, R. J., Moore, W., Hames, R., Houliston, E., Chang, P., King, M. L., Woodland, H. R., 2005. Xenopus Xpat protein is a major component of germ plasm and may function in its organisation and positioning. Developmental Biology. 287, 289-300 Hudson, C. and Woodland, H. R (1998) 'Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis', Mechanisms Of Development, 73 159 - 168 (0925-4773)

A GFP fusion with GFP at the C-terminus. Xpat-GFP for micro-injection was made by inserting the PCR

amplified ORF of GFP (primers: 5’gccgagatctATGAGTAAAGGAGAAGAAC 3’; 5’

gccggaattcTTATTTGTATAGTTCATCCAT 3’) between the BglII and EcoR1 sites of pSPJC2L(Cook et al., 1993) to give pSPJC2L-cGFP. TheORF of Xpat without the STOP

codon was amplified by PCR (primers: 5’gccgagatctATGGCTTTGAAGGCAGAAG

3’;5’gccgagatctCAGGCCAACATCCCACCAAG 3’) and inserted into the BglII site of pSPJC2L-cGFP to yield

pSPJC2 Xpat GFP. Sense RNA was transcribed from this NotI-linearized plasmid with SP6 RNA polymerase. This clone forms germ plasm-like islands in oocytes under

very specic culture conditions


transcript protein peptidylprolyl

cis/trans isomerase, NIMA-

interacting 1

prolyl isomerase Pin1, dod, ubl5

role in phosphorylation-

dependent events of cell cycle

XB-CLONE-12442079

calcium-binding actin bundling

XB-CLONE-2111736












XB-CLONE-12441973-Maya Piccinni

E601


F601


F617

XB-CLONE-12441973 -Maya Piccinni

HW-35 pix1, tuwd12, wdr51b centriole protein n/a GFP-xPoc1B GFP Pix1 laevis

HW-36 pix1, tuwd12, wdr51b centriole protein n/a GFP-xPoc1A GFP Pix2 laevis

204-SPH post Xpo nuclear transport XpoHK XpoHK no pictures laevis ? AMP

412-SPH pou3f4 TF Pou2 Pou2 no pictures p laevis ? AMP

HAR-241 ppp1r1a pil signal transduction n/a PiI Protein phosphatase tropicalis pCMV-SPO amp not full length CF147950

337 prdm1 blimp1, xblimp1 mesendoderm TF pBSXblimp1 laevis pBS SK AMP

338 prdm1 blimp1, xblimp1 mesendoderm TF pCSXbVP not needed laevis pCS 2+

241-NP prmt1-a methyltransferase 23G4-1 n/a p laevis pbs SK+ Amp

HAR-319 prox1 TF Prox1 prox-1 prox1 lymphatic n/a p tropicalis PCR2.1 topo amp

782 prox1 TF Prox1 IMAGE:4030961 no pictures p laevis pCMV-Sport6 Amp Image EST 4030961

CS-445 prph peripherin peripherin, nef4, XIF3 intermediate filament not in xenbase XIF3 XIF3 laevis pING14 Amp

896 prtn3 ela2 Trypsin Prtn3 IMAGE:7008551 no pictures p laevis pCMV-SPORT6 Amp Image EST 7008551

561 ptch1 patched 1 Hedgehog receptor Ptc1 Xptc1he3 no pictures p laevis Takabatake et al. 2001. Mech Dev 105: 197 pGEM3Zf(-) Amp contains cDNA fragment 1670-2553

578 ptch1 patched 1 Ptc1 pCS2plusXPtc1-B6 have to re do them p laevis Koebernick et al. 2001. Mech Dev 100: 303-308 pCS2+ Amp Accession No AF302765

580 ptch2 patched 2 hedgehog receptor Xptc-2/pBluescriptSK(-) Xptc-2-pBluescriptSK- no pictures p laevis Takabatake et al 2000. Mech Dev 98: 99-104 pBluescript SK(-) Amp Accession No AB037688

HAR-413 ptf1a Ptf1a/p48, XPtf1a transcription factor pt f1a IMAGE:7557710 no pics p tropicalis CS108 amp Not full length - CX496418 - 7557710

870 n/a EP4 IMAGE:5507146 no pictures p tropicalis pCMV-SPORT6 Amp Image EST 5507146, Prostaglandin receptor 4

872 ptgis n/a cytp450 synthase PGES PGES (7807114) no pictures tropicalis pCS107 Amp Image EST 7807114. Prostaglandin synthase

255-NP ptma-a prothymosin, alpha tmsa, thymosin alpha1 ubiquitin ligase prothymosin alpha (72) prothymosin alpha-72 n/a p tropicalis pBS SK(-) Amp

890 ptpn12 n/a tyrosine phosphatase PTP-Pest IMAGE:6317444 no pictures p laevis pCMV-SPORT6 Amp Image Est 6317444. Accession BC070582

583 ptprc cd45 CD45 IMAGE:4683776 no pictures tropicalis pCMV-SPORT6 Amp IMAGE EST 4683776

550-SPH pygo2 Xpygo2 transcription modulator not needed p laevis pCS2+ AMP

551-SPH pygo2 Xpygo2 transcription modulator pCS2+ Xpygo2alpha pCS2 Xpygo2alpha not needed p laevis pCS2+ AMP

poc1b [Predicted: Source is

Human Entrez Gene]

POC1 centriolar protein B


Gene]

Hames, R. S., Hames, R., Prosser, S. L., Euteneuer, U., Lopes, C. A. M., Moore, W., Woodland, H. R. and Fry, A. M.(2008) 'Pix1 and Pix2 are novel WD40 microtubule-associated proteins that colocalize with mitochondria in Xenopus germ plasm and centrosomes in human cells' Experimental Cell Research 314 (3), 574 - 589 (0014-4827)

poc1b [Predicted: Source is

Human Entrez Gene]

POC1 centriolar protein B


Gene]

Hames, R. S., Hames, R., Prosser, S. L., Euteneuer, U., Lopes, C. A. M., Moore, W., Woodland, H. R. and Fry, A. M.(2008) 'Pix1 and Pix2 are novel WD40 microtubule-associated proteins that colocalize with mitochondria in Xenopus germ plasm and centrosomes in human cells' Experimental Cell Research 314 (3), 574 - 589 (0014-4827)

posterior protein [provisional]

XB-CLONE-14574122


bug is resolved

POU class 3 homeobox 4

brn4, XlPOU2, XlPOU 2, XlPOU-2, POU 2, pou2

XB-CLONE-14574216

protein phosphatase 1,

regulatory (inhibitor) subunit

1A

PR domain containing 1, with

ZNF domain XB-CLONE-12271669



Could not add the paper to the references for some reason....

The zinc finger gene Xblimp1 controls anterior endomesodermal cell fate in Spemann's organizer (Citations: 37) F. S. J. de Souza Journal: Embo Journal - EMBO J, vol. 18, no. 21, pp. 6062-6072, 1999

PR domain containing 1, with

ZNF domain XB-CLONE-12271674

Could not add the paper to the references for some reason....

The zinc finger gene Xblimp1 controls anterior endomesodermal cell fate in Spemann's organizer (Citations: 37) F. S. J. de Souza Journal: Embo Journal - EMBO J, vol. 18, no. 21, pp. 6062-6072, 1999

fusion between the activation domain of VP16 and the zinc-finger domain of Xblimp1

protein arginine methyltransferase

1

xprmt1, anm1, hcp1, ir1b4, hrmt1l2, 3E10,

xPRMT1, xPRMT1bXB-CLONE-2421408

protein arginine methyl transferase (23G4.1)

prospero homeobox 1

Prox 1, prospero-related homeobox 1,

prospero, xprox1XB-CLONE-14578552

Prox1 fragment amplified by PCR. Cloned into topo kit vector PCR2.1 topo and is flanked by EcoRI sites.

Accession number AM922297. Not full length.

prospero homeobox 1

Prox 1, prospero-related homeobox 1,

prospero, xprox1XB-CLONE-2110458

proteinase 3 [Predicted: Source is Human Entrez

Gene]

XB-CLONE-2028142

XPtc1, patched, patched1, Ptc-1, Ptc1

XB-CLONE-12441256

XPtc1, patched, patched1, Ptc-1, Ptc1

hedgehog receptor, signal transduction

XB-CLONE-12441270

ptc-2, ptc2, patched-2, patched 2, xptc-2, xptc

2XB-CLONE-12441278

pancreas specific transcription

factor, 1aXB-CLONE-3233273

ptger4 [Predicted: Source is

Human Entrez Gene]

prostaglandin E receptor 4

(subtype EP4) [Predicted: Source is Human Entrez

Gene]


XB-CLONE-2043366

prostaglandin I2 (prostacyclin)

synthase XB-CLONE-3439397

XB-CLONE-12442380

protein tyrosine phosphatase, non-receptor type 12


Gene]

XB-CLONE-2188153

protein tyrosine phosphatase,

receptor type, Csignaling molecule



bug is resolved

pygopus family PHD finger 2


Gene]

XB-CLONE-14574225

pCS2+ NLS Xpygo2alpha pHD

pCS2 NLS Xpygo2alpha pHD

Pygopus is required for embryonic brain patterning in Xenopus.Lake BB, Kao KR.Dev Biol. September 1, 2003; 261 (1): 132-48.



Gene]

XB-CLONE-14574228













Need to be associated with the accession number-Maya Piccinni

F631

Need to be associated with the accession number -Maya Piccinni

554-SPH pygo2 Xpygo2 transcription modulator not needed p laevis pCS2+ AMP

553-SPH pygo2 Xpygo2 transcription modulator pCS2+ Xpygo2beta pCS2 Xpygo2beta not needed p laevis pCS2+ AMP

532 rara Retinoid receptor RARalpha1 pBSXRARalpha1 p laevis Kiode et al., 2001. Genes Dev 15: 2111-2121 pBluescript SK- Amp

CS-310 rara not in xenbase RARa2 RARa2 laevis pING14 Amp


CS-244 rara not in xenbase RARa2-2 RARa2-2 laevis pING14 Amp

CS-243 rara not in xenbase RARa2-2 RARa2-2 laevis pING14 Amp



CS-131 rara not in xenbase RARa2-2 RARa2-2 laevis pBluescript Amp EcoRI fragment

CS-127 rarg not in xenbase RARg2 RARgamma2 laevis pBluescript Amp EcoRI fragment

CS-335 rarg not in xenbase RARg2 RARg2 laevis pING14 Amp

1678 n/a Not in xenbase Rasip1-like2 Rasip1-like2 (8739150) n/a tropicalis pCS111 amp Image EST 8739150

1675 Rasip1-like1 Not in xenbase

HAR-217 rax TF n/a rax Xrx1, rx1, rx tropicalis CS107 amp CF239493

758 rb1 retinoblastoma 1 tumour repressor Rb1 IMAGE:6640009 yes p tropicalis pCMV-Sport6 Amp Image EST 6640009

137-AP rb1 retinoblastoma 1 tumour supressor xenopus Rb XRb tropicalis pGEM1 Amp

273-AP rb1 retinoblastoma 1 tumour supressor XRb Xrb pCS2+ tropicalis pCS2+ Amp XRb

243-AP rb1 retinoblastoma 1 tumour supressor XRb Xrb pCS2plus no pictures p tropicalis pCS2+ Amp

799 rbpj Su(H)1 IMAGE:6864463 no images p laevis pCMV-Sport6 Amp Image EST 6864463

312-AP rbpj X Su DLM X Su-H-DLM not needed p laevis pCS2+ Amp

HW-31 rela Xrel1, xrela TF n/a pSP64Txrel1 pSP64Txrel1 laevis pSP64T amp

HAR-282 rfx4 n/a TF n/a Rfx4v3.sport6 Rfx4v3 tropicalis sport6 amp Full length BQ391290

257-NP rho rhodopsin n/a p laevis pBS SK(-) Amp

777-AP rnf4 slx5, snurf, res4-26 RNF4 RNF4-pCS2plus-rep no pictures p pCS2+rep Amp PCR from IMAGE clone 7203249



Gene]

XB-CLONE-14574237

pCS2+ Xpygo2alpha NHD

pCS2 Xpygo2alpha NHD



XB-CLONE-14574234


retinoic acid receptor, alpha

xRAR alpha, rar alpha, nr1b1

XB-CLONE-12441217




transcription factor, steroid receptor class



















retinoic acid receptor, gamma

RAR-gamma, rar gamma


retinoic acid receptor, gamma

RAR-gamma, rar gamma


rasip1 [Predicted: Source is

Human Entrez Gene]

Ras interacting protein 1


Gene]

Myosin class V heavy chain

Information / Stock to come

rasip1 [Predicted: Source is Human

Entrez Gene] Myosin class V heavy

chain

PREDICTED: Xenopus (Silurana) tropicalis Ras

interacting protein 1 (rasip1)

retina and anterior neural fold homeobox

rx1, Xrx1, Xrax, Rx2A, rx, rx1a, rax-a, rax1

XRb1, retinoblastoma protein

XB-CLONE-2166259




XB-CLONE-12440837

recombination signal binding

protein for immunoglobulin kappa J region

X-Su(H), XSu(H), Su(H), suppressor of hairless,

rbpsuhnotch signal transduction

XB-CLONE-2127273


protein for immunoglobulin kappa J region

X-Su(H), XSu(H), Su(H), suppressor of hairless,

rbpsuh

signal transduction/notch signaling pathway

XB-CLONE-12440843

v-rel avian reticuloendotheliosis viral oncogene

homolog A


The ORF was inserted into the BglII site of pSP64T. Cut with BamH1 and transcribe with SP6.

regulatory factor X, 4 (influences

HLA class II expression)

rp4, opn2, xrho, rhodopsin, rh1


XB-CLONE-12442386

small Rho family member (73)

small Rho family member-73

ring finger protein 4

ubiquitin ligase modulator

XB-CLONE-12442087

laevis (7th down the list)









Have to upload pictures.-Maya Piccinni

Add as Gene Synonim-Maya Piccinni

I656

Have to upload pictures. -Maya Piccinni

G659

Add as Gene Synonim -Maya Piccinni

HAR-69 rps3a - gdf3 signalling molecule derriere TGas141f11 trops 11/17 p tropicalis CS107 amp

162 runx1 blood TF Xenopus aml p5 31 p laevis Tracey et al., 1998. Development 125: 1371-1380. pBSKII+ Runx1 P2. Trancript from promotor 2

774 runx1 blood TF Runx1 P1 IMAGE:4963637 no pictures p laevis pCMV-Sport6 Amp Image EST 4963637, transcript from promotor 1

753 runx1t1 MTG8 MTG8 XMTG8 no pictures p laevis Koyano-Nakagawa & Kintner, 2005. Dev Biol 278: 22-34 pBSII Amp

804 runx3 aml2, cbfa3, pebp2ac TF Runx3 IMAGE:8462195 not yet in xenbase p laevis pExpress1 Amp Image EST 8462195

CS-280 rxrb xRXR beta, RXRbeta not in xenbase RXRb RXRb laevis pING14 Amp

LJ-113 s1p2, xts1p2 not in Xenbase SIP2 SIP2 tropicalis Masse et al. in press, IJDB. pBSKS amp Coding Regeon + 3' UTR; 1.4kb; 1-1300

LJ-114 s1pr3 s1p3, xts1p3 SIP3 SIP3 tropicalis Masse et al. in press, IJDB. pBSSK- amp Clone Name: XL005o11. 3kb. Full Length Clone

LJ-115 s1pr5 SIP5 XL173p21 tropicalis Masse et al. in press, IJDB. pBSSK- amp Clone Name: XL173p21. 3kb. Full Length Clone

109-NP sema3a Sema 3A semaphorin XSema IIIa XSema IIIa no pictures p laevis PCR2.1 Amp

37-ER not in xenbase xNexin pCS2-xNexin laevis Int J Dev Biol 49, 781-796 Amp Xenopus nexin secreated protease inhibition

894 sfrp1 FRP p laevis pCMV-SPORT6 Amp

313 sfrp2 mgc53344, sfrp-2 XsFRP2 XB-IMG-78428 p laevis pcDNA3 Amp

22-ER sfrp2 mgc53344, sfrp-2 sFRP2 pCS2-sFRP2 laevis Mech Dev 96, 183-195 pCS2+ Amp Frizzles-related protein 2 expressed in neural plate

746 shh sonic hedgehog Xshh Xshh-pBluescript SK - p laevis pBluescript SK (-) Amp cDNA X32

HAR-12 shh sonic hedgehog SHH Tneu023N4 no pics p tropicalis CS107 amp Full Length -

HAR-18 shh sonic hedgehog SHH TNeu067o14 Trops 13/17/20/25/28 p tropicalis CS107 amp

HAR-355 shroom3 shrm, shroom, apxl3 cytoskeleton regulator n/a shroom shroom tropicalis CS107 amp not full length CX969934

HAR-371 shroom3 shrm, shroom, apxl3 cytoskeleton regulator n/a shroom CCAX5718 shroom3 no pics tropicalis CS111 amp

HAR-41 sia1 Xsia, Siamois, sia TF Siamois TGas088l16 trops 31 p tropicalis CS107 amp

HAR-349 sim1 Xsim n/a sim1 sim-1 tropicalis CS108 amp CX468007

865 sim1 Xsim TF Sim1 IMAGE:7563377 no pictures p tropicalis pCS108 Amp Image EST 7563377HAR-224 six1 SIX homeobox 1 XSix1 TF n/a Six1 six-1 six1 sine oculis tropicalis CS107 amp AL803039

63-NP six1 SIX homeobox 1 XSix1 TF p Six 1-3 pSix1-3 no pictures p laevis PGM T Easy Amp

Ribosomal protein S3A [Predicted:

Source is Human Entrez Gene] and


factor 3

fte1, mftl and derrière, derriere, vgr2, vgr-2,

gdf-3 XB-CLONE-3992793

Tgas141F11 - not full length*tested for in situ - works

runt-related transcription factor

1

Xaml, aml1 oncogene, aml-1, Runx-1, aml1,

aml, cbfa2, evi-1, amlcr1, pebp2ab,

aml1-evi-1, Xaml1

XB-CLONE-12037977

XB-IMG-78379; XB-IMG-78378


1

Xaml, aml1 oncogene, aml-1, Runx-1, aml1,

aml, cbfa2, evi-1, amlcr1, pebp2ab, aml1-evi-1, Xaml1

XB-CLONE-2248176

runt-related transcription factor 1; translocated to, 1 (cyclin D-related)


XB-CLONE-8596635


3XB-CLONE-1570771

retinoid X receptor, beta

transcriptional regulator, steroid

receptor class

s1pr2 [Predicted: Source is

Human Entrez Gene]

sphingosine-1-phosphate receptor 2


Gene]




Gene]


XB-CLONE-1998376



Gene]

edg8, s1p5, edg-8, sppr-1, sppr-2, s1p5,

xts1p5G-protein coupled


sema domain, immunoglobulin

domain (Ig), short basic domain,

secreted, (semaphorin) 3A

XB-CLONE-12442344

serpine2 [Predicted: Source is

Human Entrez Gene]

serpin peptidase inhibitor, clade E

(nexin, plasminogen

activator inhibitor type 1), member 2 [Predicted: Source is Human Entrez

Gene]

xpn-1, pn-1, pn1, glia-derived nexin, gdn, Protease nexin-1

serine protease inhibitor

secreted frizzled-related protein 1

frzA, frp, frp1, frp-1, sarp2

modulator of Wnt signaling

XB-CLONE-2198872

Follistatin related 5570309

XB-IMG-48098, have more pictures that

have to upload.Originally Image 5570729 but 100% identical to

5570309. Accession AAH43983secreted frizzled-related protein 2


XB-CLONE-12271728

Pera EM and DeRobertis EM (2000), Mech. Dev. 96 183-195

secreted frizzled-related protein 2


hedgehog, Xhh, sonic hedgehog, X-SHH,

xshh, vhh-1

intercellular signaling, early embryo

patterningXB-CLONE-12441514

XB-IMG-48617; XB-IMG-48618; XB-IMG-48619



xshh, vhh-1



Ruiz i Altaba A, Jessell TM, Roelink H. 1995. Restrictions to floor

plate induction by hedgehog and winged-helix genes in the neural

tube of frog embryos. Mol Cell Neurosci 6:106–121.


xshh, vhh-1



3' present - AL804833 - Tneu067O14*this clone has been tested and works for in situ

shroom family member 3

shroom family member 3

not full length - ES680648 - internal piece of Shroom CCAX5718

siamois homeodomain 1


Tgas088L16 - AL960285 - 5' presentT7 (not Ecor1, BamH1, or HindIII) - cla1

single-minded homolog 1


single-minded homolog 1

XB-CLONE-3225988

XB-CLONE-14575116











Sequence came back as trop-Maya Piccinni

not yet in xenbase-Maya Piccinni



have map to upload have to check them-Maya Piccinni

K669

Sequence came back as trop -Maya Piccinni

K672

not yet in xenbase -Maya Piccinni

K673

not yet in xenbase -Maya Piccinni

G675


B680

have map to upload

I680

have to check them -Maya Piccinni

HAR-232 six6 SIX homeobox 6 TF n/a six6 optx-2 optx2 tropicalis CS107 amp BX728378

HAR-147 sizzled sizzled Sizzled TNeu024k17 n/a p tropicalis CS107 amp

HAR-148 sizzled sizzled Sizzled TGas144c13 n/a tropicalis CS107 amp Full length AL963501 - Tneu024k17

719-AP skp2 xskp2 E3 ubiquitin ligase Xskp2 Xskp2 laevis pCS2+ Amp

LJ-27 slc12a1 ion transport nkcc-2 nkcc-2 no pictures p tropicalis pCMV sport 6 Amp

HAR-351 slc24a5 n/a n/a slc24a5 Slc24a5 (golden gol-1 tropicalis CS107 amp not full length 27825616

852 anion exchange Band3 IMAGE:7011228 have to upload them p laevis pCMV-SPORT6 Amp Image EST 7011228

HAR-262 slc5a8 vito, smct1 solute transport n/a Vito vito tropicalis CS107 amp AL646219

406-SPH slc5a9 sglt4, XSGLT1K solute symporter Naglucose Naglucose no pictures p tropicalis ? AMP

LJ-29 slc5a9 sglt4, XSGLT1K Glucose transporter no pictures p tropicalis pCMV sport 6 Amp

524 smad1 TGFb dependent TF pXMad no pictures p laevis pCS2 Amp

HAR-60 smad1 smad1 IMAGE:4439698 trops 24-28-31 p tropicalis CS107 amp smad1 smad-1 - Genbank ID#BG346829 - 5' present

345 smad3 TGFb dependent TF pXSmad3 n/a p laevis pBSK Amp

17-CC smad4.1 TGFb TF Smad4-alpha Smad4-alpha not needed p laevis pCS105 Amp Signal Transducers

165-KL TGFb TF Smad10 pCS2plus Smad10 not needed p laevis Ren and Graff, Development pCS2+ Amp Xenopus cDNA for Neuralisation

18-CC TGFb TF Smad4-beta Smad4-beta not needed p laevis pCS105 Amp Signal Transducers

HAR-117 n/a SMAD10 tropicalis CS107 amp

HAR-322 smad6 madh6, Xsmad6 heart/gut TF n/a Smad6 smad6 smad-6 tropicalis pCMV-SPO amp Full length AL636391

HAR-323 smad6 madh6, Xsmad6 heart/gut TF n/a Smad6 smad6 smad-6 tropicalis pCMV-SPO amp Full length AL636391

19-CC smad6 madh6, Xsmad6 TGFb TF Smad6 Smad6 not needed p laevis pCS105 Amp Signal Transducers

20-CC smad7 xsmad7 TGFb TF Smad7 Smad7 not needed p laevis pCS105 Amp Signal Transducers

579 smo Smo5' pGEMT-XSmo5 prime no pictures p laevis Koebernick et al. 2001. Mech Dev 100: 303-308 pGEMT Amp Accession No AF302766, contains 185bp 5'UTR

HAR-372 smo n/a smo no pics tropicalis CS108 amp

802 n/a BOP2 BOP2 (4970038) yes p laevis pCMV-Sport6 Amp Image EST 4970038. Full length. Accession BC072803

831 snai1 snail homolog 1 neural crest TF Snail pMX51-cDNA-Xsna no images p laevis From Roberto Mayor pSP72 Amp From Roberto Mayor

AP-23 snai1 neural crest TF XSnail Xsnail laevis

HAR-35 snai2 neural TF snai2 (Slug) TNeu008a21 p tropicalis CS107 amp

XOptx2, Six6.1, Six6.2, xSix6, optx2

secreted frizzled, xszl, sizzled, frzb3


XB-CLONE-4339291

Xenopus tropicalis sizzled. Full length. Accession number AL639345.



XB-CLONE-3999394

S-phase kinase-associated protein

2, E3 ubiquitin protein ligase

solute carrier family 12

(sodium/potassium/chloride

transporters), member 1

XNKCC2, Na-K-2Cl cotransporter, NKCC2,

NKCC-2XB-CLONE-11921831

solute carrier family 24, member

5transmembrane solute

exchange

slc4a1 [Predicted: Source is

Human Entrez Gene]

solute carrier family 4, anion

exchanger, member 1

(erythrocyte membrane protein

band 3, Diego blood group)


Gene]

ae1, bnd3, cd233, empb3, epb3, rta1a,

wd1, band 3XB-CLONE-2030312


(sodium/monocarboxylate

cotransporter), member 8


(sodium/sugar cotransporter),

member 9

XB-CLONE-14574210


(sodium/sugar cotransporter),

member 9

Na+Glucose transporter

XB-CLONE-12441993

Na+Glucose transporter

SMAD family member 1

Xsmad1, smad-1, Xmad1, MLP

XB-CLONE-12441213

Xenopus mothers against decapentaplegic is an embryonic ventralizing agent that acts downstream of

the BMP-2/4 receptor.Thomsen GH.

Development. August 1, 1996; 122 (8): 2359-66. -----------------

Xenopus Mad proteins transduce distinct subsets of signals for the TGF beta superfamily.

Graff JM, Bansal A, Melton DA.Cell. May 17, 1996; 85 (4): 479-87.

very close in sequence to XMad1 of Graf et al., Cell 1996, but some nucleotide and amino acid differences.

Possibly Xmad and XMad1 are alleles


Xsmad1, smad-1, Xmad1

signal transduction, TGF-beta pathway

XB-CLONE-4465335


XSmad3, Xmad3, XenMLP

XB-CLONE-12440833

SMAD family member 4, gene 1

XSmad4alpha, Xsmad4, jip, dpc4,

madh4XB-CLONE-12442201

smad4.2 [provisional]


[provisional]

XSmad4alpha, xsmad4, jip, dpc4, madh4,

smad10XB-CLONE-12442528



[provisional]

XSmad4alpha, xsmad4, jip, dpc4, madh4,

smad10XB-CLONE-12442204



[provisional]

XSmad4alpha, xsmad4, jip, dpc4, madh4, smad10

signal transduction and TF




XB-CLONE-12442207


XB-CLONE-12442210

smoothened, frizzled family

receptorXSmo, X-smoothened,

smoothenedhedgehog signal

transductionXB-CLONE-12441275

smoothened, frizzled family

receptorXSmo, X-smoothened,

smoothenedhedgehog signal

transductionSmoothened XZG33297

not full length - CX490515 - internal piece of Shroom CCAX5718

smyd1 [Predicted: Source is

Human Entrez Gene]

SET and MYND domain containing

1 [Predicted: Source is Human

Entrez Gene]

redicted histone tail methylase containing

SET domainXB-CLONE-2032560

xSna, snail, sna, Xsnail, snail1

XB-CLONE-12441737

snail family zinc finger 1

xSna, snail, sna, Xsnail, snail1

snail family zinc finger 2

xslug, xSlu, slug, Snail2, XSnail2

XB-CLONE-4344467

trops 17/20/26/ 29/30/33/34

Tneu008A21 - AL635334 - 5' presentLikely have 3' also
















sugest as synonim-Maya Piccinni


G713

sugest as synonim -Maya Piccinni

I713


832 snai2 snail homolog 2 neural crest TF Slug348 pMX348-47 Xslug not yet in xenbase p laevis From Roberto Mayor pSP72 Amp From Roberto Mayor

833 snai2 snail homolog 2 neural crest TF Slug363 pMX363 Xslug not yet in xenbase p laevis From Roberto Mayor pSP72 Amp From Roberto Mayor

922 snrk n/a Snrk Snrk (7676594) no pictures p tropicalis pCMV-SPORT6 Amp Image EST 7676594

HAR-134 sox1 XSox1 TF sox1 TNeu023i18 trops 15/15/20/20/24 p tropicalis CS107 amp 5' absent, not full length. Accession number AL642062

HAR-135 sox1 XSox1 TF sox1 TGas002m12 n/a p tropicalis CS107 amp Full length. Accession number AL631116

HAR-320 sox1 XSox1 TF Sox1 Sox1 truncated UTRs n/a p tropicalis pBS bluescript amp

HAR-321 sox1 XSox1 TF Sox1 Sox1 truncated UTRs-1 n/a p tropicalis pCS108 amp

HAR-209 sox10 n/a TF sox10 TNeu062e04 trops 18/19/25/28/28 p tropicalis CS107 amp Accession number AL673575.

HAR-275 sox10 n/a Tf n/a Sox-10 tropicalis

HAR-317 sox14 n/a TF n/a Sox 1 4 tropicalis CS107 amp AM922298

HAR-57 sox15 TF Sox-D IMAGE:4439734 trops 19/23/28/31 p tropicalis CS107 amp

134 sox17a pBSXsox17 alpha pBSXsox17alpha n/a p laevis Hudson et al. (1997) Cell 91, 397-405 pBluescript SK AMP

133 sox17b.1 pBSXsox17 beta n/a p laevis Hudson et al. 1997 Cell 91, 397-405 pBluescript SK AMP

HAR-58 sox17b.1 TF Sox17beta IMAGE:4463388 n/a p tropicalis CS107 amp

HAR-238 sox17b.1 TF n/a Sox17-b sox17beta sox17-beta tropicalis pCMV-SPO amp BQ392386

43-ER sox17b.1 TF Sox17b pGEM-T Sox17b laevis Development 127,2053-2062 pGEM-T-easy Amp Xenopus Sox17 endoderm Marker

842 sox18 TF Sox18b IMAGE:4175390 no pictures p laevis pCMV-SPORT6 Amp Image EST 4175390, Accession BC072123

HAR-75 sox2 neural TF Sox 2 TGas061h22 trops 15/16/20/28 p tropicalis CS107 amp AL680500 - Tgas061h22

HAR-76 sox2 neural TF Sox-2 TNeu054c10 n/a p tropicalis CS107 amp

1047-AP sox2 TF XSox2 pCS2plus-Sox2 no pictures p tropicalis pCS2+ Amp

42-ER sox2 TF not in xenbase pBS SOX2 pBS SOX2 tropicalis Cold spring Habour symp Quant Biol 62,169-175 pSK Amp Pan Neural Xenopus Probe Sox2

HAR-316 sox21 n/a TF n/a Sox 2 1 Sox 21, sox-21, tropicalis CS107 amp AL677908

HAR-59 sox3 neural TF sox-3 IMAGE:4463920 p tropicalis CS107 amp


XB-CLONE-12441740


XB-CLONE-12441743

SNF related kinase [Predicted: Source is Human Entrez

Gene]

Serine/threonine protein kinase

XB-CLONE-3611347

SRY (sex determining region

Y)-box 1XB-CLONE-4335161





the Sox1 coding region was excised from plasmid #135 (AL595157) with Hind III and Xba 1. This should remove a lot of the 5' and 3' UTRs. The Sox1 gene was inserted into pBS using the Hind III and Xba1 sites. I want to test

if the UTR regions of the trop Sox1 gene are suppressing activity. This version has most of the UTRs removed. I will use this to transfer the Sox1 gene into

pCS108 using Sal1 and Not1 which will then be used for mRNA production.



The Sox1 coding region was excised from plasmid #320

with Sal1 and Not 1. This clone of Sox1 has removed a lot of the

5' and 3' UTRs. I want to test if the UTR regions of the trop Sox1 gene are suppressing activity. This version has most of the

UTRs removed. I will use this to test if the Sox1 gene is now active in embryo assays.SRY (sex

determining region Y)-box 10

XB-CLONE-4262745


Y)-box 10 SRY (sex


XtSox14, Sox 14, Sox-14


Y)-box 15 [Predicted: Source is Human Entrez

Gene]

sox20, sox26, sox27, sox-d, soxd, xsoxd

XB-CLONE-4465410

sox soxD sox D sox-D - Genbank ID# BG348615 - Uncertain



Y)-box 17 alpha

xSox17alpha1, xSox17alpha2,

tSox17alpha, Xsox17-alpha, Sox17alpha,

sox17-alpha, xsox17a, sox17, xsox17,

Xsox17alpha, sox17 alpha

HMG-box transcription factor

XB-CLONE-11952535


Y)-box 17 beta, gene 1

Xsox17-beta, sox17-beta, tSox17beta, Sox17beta, sox17,

Xsox17

HMG-box transcription factor

XB-CLONE-11937901




Xsox17

XB-CLONE-4457439

sox sox-17 sox17 xsox17 beta - Genbank ID#BG886038

**this clone has been tested and works for in situ




Xsox17




Xsox17SRY (sex


xSox18alpha, xSox18beta, xsox18

XB-CLONE-2293251


Y)-box 2

XLSOX-2, Sox-2, XSox2, Xsox-2, anop3,

mcops3XB-CLONE-4152200


Y)-box 2



Tneu054c10NOTE: This clone gives tons of background when used

for in situ probe. (ClaI/T7)Use #75 instead


Y)-box 2




Y)-box 2


mcops3SRY (sex



Y)-box 3xSox3, Sry, Xtsox3,

Xlsox3XB-CLONE-4462787

trops 13/13/14/14/14/ 16/17/17/24/24/ 26/28/29/30/35/

36/37/38

sox-3 sox3 sox xsox3 - Genbank ID# BG592766 - Full Length


















Usto be XB-CLONE-12441961-Maya Piccinni

F719

Usto be XB-CLONE-12441961 -Maya Piccinni

23-NP sox3 TF X Sox 3 X Sox 3 laevis Blue Script Sk+ Amp Insert 1.6kb into Eco R1 site. Plasmid size 4.5 Kb

841 sox7 xSox7 TF Sox7 IMAGE:4740338 no pictures p laevis pCMV-SPORT6 Amp Image EST 4740338

25-KL sox9 TF Xsox9 pGEMT Xsox9 pGEMT no pictures p laevis pGEMT easy 3kb Amp Sox9

348 spam1 XEH1, PH20 sperm adhesion pXEH1 p laevis GenBank Accession number AF134981 pBS Amp

1540 spi1 pu.1, sfpi1, spi-1, spi-a Not in xenbase PU1 Spi1 (7002682) n/a tropicalis pExpress1 Amp

582 spib spi-b ETS transcription factor SpiB SpiB (5537169) have to re do them p laevis pCMV-SPORT6 Amp IMAGE EST 5537169, Accession number BC046671

HAR-392 spred1 Xtspred1, Xspred1 n/a Spred1 tropicalis CS107 amp Full length NM_0010080 7877785

722 spry2 sprouty homolog 2 FGF signal modulator Xspry2 pCS2 Xspry2 delta not needed p laevis pCS2+ Amp

HAR-391 spry2 sprouty homolog 2 n/a Sprouty 2 spry2 tropicalis CS107 amp Full length CR761255

346 stat3.1 Xstat3, aprf, hies neural crest TF pXStat3 p laevis pBluescript II KS- Amp

556 stat3.1 Xstat3, aprf, hies intracellular signaling STAT3En Xstat3EnR not needed p laevis Nishinakamura et al. 1999. Dev Biol 216: 481-490 pBluescript II SK- Amp 5' HA tag

557 stat3.1 Xstat3, aprf, hies intracellular signaling STAT3En-Mut Xstat3EnR-Mut not needed p laevis Nishinakamura et al. 1999. Dev Biol 216: 481-490 pBluescript II SK- Amp Inactive form of Xstat3EnR, mutations E to A, V to A

347 stat5b stat5 blood TF pXStat5 p laevis pBluescript II SK Amp

418-SPH sulf1 sulfatase 1 XtSulf1, XtSulf-1 XtSulf1 XtSulf1 no pictures p tropicalis pBSK+ AMP 700bp EcoRI fragment of XtSulf1 in pBSK+

756 hrd1 E3 ubiquitin ligase Synoviolin1 IMAGE:6639046 no pictures p laevis pCMV-Sport6 Amp Image EST 6639046, Accession BC070731

40-ER szl sizzled Sizzled pCS2 Sizzled laevis Nat Rev Mol Cell Biol 7,296-302 pCS2+ Amp Sizzled untagged full length

41-ER szl sizzled not in xenbase Sizzled-HA Sizzled-HA laevis Cell 124,147-159 pCS2+ Amp HA tag at c-terminus

10 t mesoderm TF Xbra Xbra no p laevis psp73 Amp Cut with T1 Rnase alone, 42oC, 40mins

HAR-24 t mesoderm/notocord TF t TNeu024f07 trops 10 p tropicalis CS107 amp xbra x-bra brachyury - Full Length - AL642113

HAR-30 t mesoderm/notocord TF t tropicalis CS107 amp

HAR-33 t notocord TF t TGas020f02 n/a p tropicalis CS107 amp

67-NP t not known not in xenbase pXT 1 pXT 1 no pictures laevis Amp

366 t mesoderm TF PSP64T Xbra-EnR not needed p laevis pSP64T Amp

563 tab1 map3k7ip1 ser/thr phosphatase TAB1 xTAB1-pBSSK- no pictures p laevis Shybuya et al. 1998. EMBO J 17: 1019-1028 pBluescript SK- Amp May be also for injections


Y)-box 3 xSox3, Sry, Xtsox3,

Xlsox3




Y)-box 9cmd1, sox-9, sra1,

cmpd1XB-CLONE-12442420

sperm adhesion molecule 1 (PH-20

hyaluronidase, zona pellucida

binding) [Predicted: Source is Human Entrez

Gene]

XB-CLONE-12440854


XB-IMG-78434

spleen focus forming virus

(SFFV) proviral integration oncogene

ETS transcription factor Image EST 7002682. Full length clone, Accession BC098077

Spi-B transcription factor (Spi-1/PU.1

related) XB-CLONE-2203856

sprouty-related, EVH1 domain containing 1

regulation of signal transduction

Xsprouty2, sprouty2, sprouty-2, sprouty 2, Xtsprouty2, xspry2

XB-CLONE-12441327

inhibits FGF signalling IMAGE EST 5513422 in pCMV.SPORT6

Xsprouty2, sprouty2, sprouty-2, sprouty 2, Xtsprouty2, xspry2

regulation of signal transduction

signal transducer and activator of transcription 3 (acute-phase

response factor), gene 1

XB-CLONE-12440839




XB-CLONE-8596625



XB-CLONE-12441247

signal transducer and activator of transcription 5B

[provisional]

XB-CLONE-12440845



sulfatase, modulates signalling

XB-CLONE-14574219

syvn1 [Predicted: Source is

Human Entrez Gene]

synovial apoptosis inhibitor 1, synoviolin


Gene]

XB-CLONE-2167925





T, brachyury homolog

Xbra, X-bra, bra, Xbrachyury, brachyury,

ntl XB-CLONE-11536564



ntl, buXB-CLONE-4335073



ntl, bu

xbra BRA XBRA Xbra bra - Full length - AL628556 - Tgas023E12 - this clone has been tested and works for

in situ



ntl, buXB-CLONE-4133382

xbra XBRA bra Xbra - Tgas020F02 - 5' present - Maybe have 3' of gene - Al633024



ntl



ntl XB-CLONE-12440896

Conlon et al.,Development 122, 2427-2435Badiani et al., Gand D 8, 770-782

Construct geneated by destroying the initial methionine in engrailed, derived from the plasmid PMEnT (Badiani

et al) and replacing it with a Cla 1 site. The construct is myc tagged via HindIII and a short oligo links myc to the

engrailed repressor. The

TGF-beta activated kinase

1/MAP3K7 binding protein 1

XB-CLONE-12441263


















usto be XB-CLONE-6541321-Maya PiccinniSequence alightment with different gene-Maya Piccinni

F745


F749

Sequence alightment with different gene -Maya Piccinni

228 tal1 blood/vascular TF XSCL Xscl p laevis pGEM7 amp

279 tal1 blood/vascular TF pSCL not needed p laevis pGEM-HE Amp

860 tbx1 T-box 1 TF Tbx1b IMAGE:7206683 no pictures p laevis pExpress1 Amp Image EST 7206683, Accession BC108602

448 tbx2 T-box 2 Xltbx2, xtbx2 tbx2 no pictures p laevis

552 tbx20 T-box 20 XTBX20 Heart TF Tbx20 XB-IMG-48712 p laevis Brown et al. 2003. Dev Genes Evol 212: 604-607 pGEM T-Easy Amp Does not have full 5' UTP, stop codon or 3' UTR

449 tbx3 T-box 3 xtbx3, Xltbx3 tbx3 no pictures p laevis pBluescript SK(-) amp

451 tbx5 T-box 5 xtbx5, tbx-5, xtbx-5 heart and limb TF tbx5 n/a p tropicalis pGem-T

594-AP tbx5 T-box 5 xtbx5, tbx-5, xtbx-5 TF XTbx5 not needed p laevis pCS2+ Amp

HAR-458 tcf15 paraxis paraxis IMAGE:7514207 no pictures p tropicalis pCS22+ amp CX743814, IMAGE 7514207

LJ-9 tcf21 capsulin, pod 1, pod1 gut/kidney TF MyC - POD1 pCS3+ MyC - POD1 pCS3 plus not needed p laevis pCS3+ Amp Myc tagged XPod1

89-SPH tcf3 TF XTcf-3 XTcf-3 not needed laevis Molenaar et al 1996, Cell 86 391 pT7TS AMP

90-SPH tcf3 TF NXTcf-3 NXTcf-3 not needed laevis Molenaar et al 1996, Cell 86 391 pT7TS AMP

2-KL tcf3 XE60 XE60 no pictures p laevis Molenaar et al., Cell, 1996 pT7Ts Amp Expression construction for dominant negative Xtcf3

LJ-7 tcf3 TF E12 pSP64T E12 pSP64T not needed p laevis pSP64T Amp

AP-16 tcf3 transcription cofactor XE12 PSP64X beta-m-XE12 not needed p laevis psp64t Amp

281-SPH tcf7 tcf1, Xtcf1, Xtcf-1, tcf-1 TF pT7TS-XL-Tcf1E pT7TS-XL-Tcf1E not needed tropicalis pT7TS AMP

278-SPH tcf7 tcf1, Xtcf1, Xtcf-1, tcf-1 TF pT7TS-XL-Tcf1B pT7TS-XL-Tcf1B not needed laevis pT7TS AMP Have a BamHI site at 500bp.

279-SPH tcf7 tcf1, Xtcf1, Xtcf-1, tcf-1 TF pT7TS-XL-Tcf1C pT7TS-XL-Tcf1C not needed laevis pT7TS AMP Has a BamHI site at 500bp.

280-SPH tcf7 tcf1, Xtcf1, Xtcf-1, tcf-1 TF pT7TS-XL-Tcf1D pT7TS-XL-Tcf1D not needed laevis pT7TS AMP Has a BamHI site at 500bp.

LJ-51 tcf4, XTCF-4, tcf4e dntcf dntcf not needed p laevis Koriek et al., 1997. pT7TS Amp

HAR-300 tek tie2, tie-2, Xtie2 RTK n/a tek tie-2 tie2 vascular, tek Trops 28 tropicalis CS107 amp

931 tesc chp3 Tescalcin Tescalcin (7828767) no pictures p tropicalis pCS107 Amp Image EST 7828767, Accession BC121662

HAR-412 tf transferrin iron homeostasis n/a transferrin no pics tropicalis CS107 amp DN024265 - 3' present

754 tfrc n/a ATP/GTP binding CD71 IMAGE:5078994 no pictures p tropicalis pCMV-Sport6 Amp Image EST 5078994. Also called Ferroportin receptor

T-cell acute lymphocytic leukemia 1

SCL, tal-1, bHLHa17, tcl5, stem cell

leukaemia XB-CLONE-12070227

XB-IMG-48598XB-IMG-48599XB-IMG-48653XB-IMG-48654XB-IMG-48655

SCL cDNA fragment from nucelotide 540 to nucleotide 1880

T-cell acute lymphocytic leukemia 1

SCL, tal-1, bHLHa17, tcl5, stem cell

leukaemia XB-CLONE-12271677

Mead, P.E. et al., SCL specifies hematopoietic mesoderm in Xenopus embryos. 1998. Development. 125:2611-

2620.

xtbx1, cafs, cthm, dgcr, dorv, vcfs, tbx1c

XB-CLONE-1818366

T-box transcription factor

XB-CLONE-12441587

XB-CLONE-12441233

pXTbx20D5prime-3prime

T-box transcription factor

XB-CLONE-12441174

XB-CLONE-14575382

XB-CLONE-11536285

Xenopus Tbx5-pCS2plus

transcription factor 15 (basic helix-

loop-helix)helix-loop-helix



XB-CLONE-11536599


e2a, itf1, tcf-3, xtcf3, xtcf-3, vdir, bhlhb21,

tcfe2aXB-CLONE-12564837


bug is resolved





bug is resolved

Contains the 5 prime and 3 prime RTRs of Xenopus Beta Globin mRNA.

Contains a multimerized HA-Tag at the N-terminus.

transcription factor 3 (E2A

immunoglobulin enhancer binding factors E12/E47)


tcfe2ahelix-loop-helix



immunoglobulin enhancer binding factors E12/E47)




immunoglobulin enhancer binding factors E12/E47



transcription factor 7 (T-cell

specific, HMG-box) XB-CLONE-14574180


bug is resolved

Inserted in EcoR V site, has aBamH I site at ~500bp,

a Hind III site at ~1400bp, a EcoR I site right after the stop codon.




bug is resolved




bug is resolved




bug is resolved

tcf7l2 [provisional]

transcription factor 7-like 2 (T-cell

specific, HMG-box) [provisional]

high mobility group transcription factor

XB-CLONE-12442044

TEK tyrosine kinase, endothelial

tie-2 tie2 vascular, tek tyrosine kinase - DEFINITION JGI_CABE1423.fwd NIH_XGC_tropOva1 Xenopus

tropicalis cDNA clone IMAGE:7820350 5', mRNA sequence.

ACCESSION DN086803VERSION DN086803.1 GI:59752081

Site_1: EcoRI; Site_2: XhoI

tescalcin [Predicted: Source is Human Entrez

Gene]

Ca2+/calmodulin-dependent protein

phosphatase (calcineurin subunit B), EF-Hand superfamily

protein

XB-CLONE-3381240

transferrin, serotransferin

precursor

transferrin receptor (p90, CD71)

XB-CLONE-2152913



















HAS NO VECTOR ASSOCIATED FOR IT-Maya Piccinni



F767

HAS NO VECTOR ASSOCIATED FOR IT -Maya Piccinni

E771


F771


HAR-297 tie1 n/a ser/thr kinase receptor n/a Tie1 tie-1 tie1 vascular tropicalis CS107 amp

HAR-301 tie1 n/a ser/thr kinase receptor n/a Tie-1 tie-1 tie1 vascular tropicalis pCMVspor amp CX909068

HAR-303 tie1 n/a ser/thr kinase receptor n/a Tie1 tie-1 tie1 tropicalis pCMVspor amp CX913408

80-SPH tnni3 cardiac troponin pXTn Ic pXTn Ic no pictures laevis Drysdale et al. 1994, Dev Biol, 165, p432-441 pBSK AMP Full length Xenopus cardiac troponin I cDNA

LJ-26 tnni3 cytosolic contractile Cardiac Tropomyosin Cardiac Tropomyosin no pictures p laevis pGEMT Amp

HAR-358 tp63 tumor protein p63 transcription factor n/a p 6 3 tumor protein p63 tropicalis CMV sport6 amp 3' present CX912693

417-AP tp63 tumor protein p63 transcription factor not in xenbase Xp63 Xenopus p63-wt no pictures p laevis pSP64TS Amp Xenopus p63

HAR-332 trim33 ubiquitin-protein ligase Ectodermin TNeu104j16 no tropicalis CS107 amp NOT Full length. Accession number AL786185.

HAR-181 Alpha tubulin tuba tubulin a-tubulin alpha tropicalis pCMV-sport6.1 amp

HAR-269 tuba4a tubulin, alpha 4a tuba1 cytoskeleton n/a tuba alpha tubulin tropicalis pCMV-SPO amp BC061297

65-KL tubb tubulin, beta class I XLOT, tubulin beta-1 microtubule component k2053 Beta tubulin ClassII no pictures p laevis pBSKS+ Amp

733 tubb2b cytoskeletal protein Tubulin IMAGE:5571885 have to upload them p laevis pCMV-SPORT6 Amp

HAR-52 tubb2b cytoskeleton TNeu143i02 trops 22 p tropicalis CS107 amp

350 twist1 neural crest TF pBS-Xtwi18 p laevis NDH et al. (1989) Cell 59, 893-903 Bluescribe Amp

HAR-44 twist1 Xtwi, X-twi, twist, Xtwist neural TF Twist1 TNeu085g21 trops 13/19/22/28 p tropicalis CS107 amp Tneu085G21 - AL787099 - 5' present

HAR-45 twist1 Xtwi, X-twi, twist, Xtwist neural TF Twist1 Tneu125e01 n/a p tropicalis CS107 amp Tneu125e01 - Full Length

HAR-47 twist1 Xtwi, X-twi, twist, Xtwist neural TF Twist1 TNeu076a05 n/a p tropicalis CS107 amp Tneu076A05 - AL785577 -3' present

HAR-48 twist1 Xtwi, X-twi, twist, Xtwist neural TF Twist1 TNeu143k24 n/a p tropicalis CS107 amp Tneu143K24 -AL798400 - Full Length

HAR-49 twist1 Xtwi, X-twi, twist, Xtwist neural TF Twist1 TNeu075h18 n/a p tropicalis CS107 amp

HAR-50 twist1 Xtwi, X-twi, twist, Xtwist neural TF Twist1 TNeu099j15 n/a p tropicalis CS107 amp Tneu099J15 - AL791808 - Full Length

HAR-51 twist1 Xtwi, X-twi, twist, Xtwist neural TF Twist1 TNeu141p24 n/a p tropicalis CS107 amp Tneu141P24 - AL799063 - Full Length

62-KL twist1 Xtwi, X-twi, twist, Xtwist neural TF pXtwi560 pXTwi560 no pictures p laevis Hopwood pBSKs+ Amp Twist

HAR-88 twsg1 signalling modulator twsg1 TGas066i20 N/A p tropicalis CS107 amp

HAR-91 twsg1 signalling modulator twsg1 TGas012m12 trops 23/28 p tropicalis CS107 amp AL634092 - Tgas012m12

9-CC twsg1 not needed p laevis pCS105 Amp Extracellular Regulators

tyrosine kinase with

immunoglobulin-like and EGF-like

domains 1 tyrosine kinase

with immunoglobulin-like and EGF-like

domains 1 tyrosine kinase

with immunoglobulin-like and EGF-like

domains 1

troponin I type 3 (cardiac)

cardiac troponin I, TnIc, ctnIc, cTNT, cardiac tropin T, XTnIc, c-

troponin

XB-CLONE-14573976


bug is resolved

troponin I type 3 (cardiac)

cardiac troponin I, TnIc, ctnIc, cTNT, cardiac tropin T, XTnIc, c-

troponin

XB-CLONE-11920650

trp63, ket, tp73l, Xp63, p63, np63

trp63, ket, tp73l, Xp63, p63, np63

Lu and Vize eXenopus p63 expression in early ectoderm and neurectoderm.

Lu P, Barad M, Vize PD.Mech Dev. April 1, 2001; 102 (1-2): 275-8.t al (2001)

Mech

tripartite motif containing 33

ptc7, rfg7, tf1g, tif1g, tifgamma, tif1gamma,

ectodermin, tif1-gamma

XB-CLONE-4249129

tuba1a [Predicted: Source is

Human Entrez Gene]

tubulin, alpha 1a [Predicted: Source is Human Entrez

Gene]

lis3, tuba3, b-alpha-1, alphatub84

XB-CLONE-4414096 but also HAR-213

>gi|33245881|gb|CF149613.1|CF149613 AGENCOURT_14929401 NICHD_XGC_Emb7 Silurana

tropicalis cDNA clone IMAGE:6980422 5'.

We thought this was gridlock but seq. confirms that it is tubulin

XB-CLONE-12442467

Gene expression in the embryonic nervous system of Xenopus laevis.

Richter K, Grunz H, Dawid IB.Proc Natl Acad Sci U S A. November 1, 1988; 85 (21):

8086-90.

Beta tubulin class II neural specific Xenopus cDNA EcoRI insert

tubulin, beta 2B class IIb

n-tubulin, ntubulin, Xn-tubulin, N-Tub

XB-CLONE-2192914

Image EST 5571885, full length cDNA. Accession AAH44030

tubulin, beta 2B class IIb

n-tubulin, ntubulin, Xn-tubulin, N-Tub

XB-CLONE-4280366

tubb (Neural Specific Tubulin)

Tneu143I02 - AL798377 - 5' presentNote this clone is chimeric. The 5' end of this clone is

the full length tropicalis neural specific tubulin (~1.7kb). However a portion (about 1.1kb) of 18S

sequence is fused to the 3' end of the tubulin clone (same orientation). Therefore this clone is NOT suitable

for in situs.

twist family bHLH transcription factor

1 Xtwi, X-twi, twist,

Xtwist XB-CLONE-12440865

XB-IMG-48642XB-IMG-48641 XB-IMG-

48609

twist basic helix-loop-helix


XB-CLONE-4250578



XB-CLONE-14575067



XB-CLONE-4263023



XB-CLONE-4280328



XB-CLONE-4263263

Tneu075H18 - AL776347 Full Length*this clone has been tested and works for in situ



XB-CLONE-4248325



XB-CLONE-4279433



XB-CLONE-12442459

twisted gastrulation homolog 1

tsg, twisted gastrulation, twsg

XB-CLONE-4155924

AL682499 - Not full Length - Tgas066i20THIS APPEARS TO BE A CHIMERIC CLONE. USE RECORD

#91 INSTEAD!twisted

gastrulation homolog 1


XB-CLONE-4274312



extracellular modulator of BMP activity

XB-CLONE-12442177

Tsg (twisted gastrulation)

Tsg - twisted gastrulation




















G792


45-ER twsg1 xTSG pCS2-xTSG laevis Development 128,4439-4447 pCS2+ Amp

44-ER twsg1 not in xenbase xTSG pCDNA xTSG laevis Nature 405, 757-763 pCDNA3 Amp Xenopus twisted gastrulation untagged

HAR-233 signalling modulator n/a TSG2 tsg2 tsg-2 twisted tropicalis pCMV-sport amp CF593113

HAR-157 TF forkhead TGas019f24 n/a p tropicalis CS107 amp

337-NP upib, upk1, tspan20 membrane protein tropicalis pBS SK(-) Amp

767 n/a UROS IMAGE:7206195 no pictures p laevis pExpress1 Amp Image EST 7206195, Heme biosynthesis enzyme

464 vangl2 XStbm-CS2 not needed p laevis CS2 4.1kb amp

780 vav Vav1 IMAGE:5513206 no pictures p laevis pCMV-Sport6 Amp Image EST 5513206

917 vav2 n/a Vav2 Vav2 (4724847) p laevis pCMV-SPORT6 Amp Image EST 4724847, Accession BC057726

836 n/a Vav3 IMAGE:4964014 no pictures p tropicalis pCMV-SPORT6 Amp Image EST 4964014,

139 vegfa vegf, vegf-a vascular growth factor XenopusVEGF1 cDNA VEGF-9 2 p p laevis Cleaver at al (1997) Dev. Dyn. 210, 66-77(Paul Kreig) pBS sk+

719 vegfa vegf, vegf-a peptide growth factor VegfA pCS2plus-VegfA-S not needed p laevis Cleaver et al 1997. Dev Dyn 210:66-77 pCS2+ Amp Xenopus VegfA Small isoform, Accession AF008593

720 vegfa vegf, vegf-a peptide growth factor VegfA pCS2plus-VegfA-M not needed p laevis Cleaver et al 1997. Dev Dyn 210:66-77 pCS2+ Amp Xenopus VegfA Medium isoform, Accession DQ481238

721 vegfa vegf, vegf-a peptide growth factor VegfA pCS2plus-VegfA-L not needed p laevis Cleaver et al 1997. Dev Dyn 210:66-77 pCS2+ Amp Xenopus VegfA Large isoform, Accession AF008595

HAR-178 vegf, vegf-a signalling molecule VegF TEgg015l19 tropicalis CS107 amp not full length AL852176

HAR-93 mesendoderm TF VegT tropicalis CS107 amp AL683477

HAR-94 mesendoderm TF VegT TGas032n19 n/a p tropicalis CS107 amp TGas032N19 - AL648546

HW-17 TF n/a pSPVegT pSPVegT laevis pSP64T amp

HW-19 TF n/a pSPVegTEnR pSPVegTEnR laevis pSP64T amp

HW-20 TF n/a pSPFullLengthVegTEnR pSPFullLengthVegTEnR laevis pSP64T amp

HW-23 TF n/a pSPVegTMyc pSPVegTMyc laevis pSP64T amp

twisted gastrulation BMP

signaling modulator 1



xTwisted gastrulation-flagtagged, for expression in embryos

twisted gastrulation BMP

signaling modulator 1



twsg2 [provisional]


[provisional]

xTsg-2, Tsg-2, Tsg2, xTsg2

unnamed gene in xenbase

XB-CLONE-4142774 but

does not accept that is

from EXRC

Tgas019F24This clone has weak similarity to XFD2 (FoxI1a)

upk1b [Predicted: Source is

Human Entrez Gene]

uroplakin 1B [Predicted: Source is Human Entrez

Gene]

XB-CLONE-12442397

pbluescript sk (-) Uroplakin 1b

pbluscript sk- Uroplakin 1b

uros [Predicted: Source is

Human Entrez Gene]

uroporphyrinogen III synthase


Gene]

Uroporphyrinogen III synthase UROS/HEM4

XB-CLONE-1822350

VANGL planar cell polarity protein 2 [Predicted: Source is Human Entrez

Gene]

stb1, Strabismus, lpp1, ltap, stbm, stbm1, Van

Gogh, vang-like 2

Non-canonical wnt pathway signaling

protein XB-CLONE-12441188

Xstbm-pBS (clone 2A) sequentially digested with Bgl2 site found in 3'UTR, blunt, and EcoR1. Fragment cloned

into CS2 polylinker at EcoR1 and Stu1 sites.

vav1 [Predicted: Source is

Human Entrez Gene]

vav 1 guanine nucleotide

exchange factor [Predicted: Source is Human Entrez

Gene]

guanine nucleotide exchange

XB-CLONE-2047088



Gene]

guanine nucleotide exchange factor

XB-CLONE-2203028


vav3 [Predicted: Source is

Human Entrez Gene]



Gene]

guanine nuceotide exchange factor

XB-CLONE-2254160

vascular endothelial growth

factor A [provisional]

XB-CLONE-12007248

Coding region is 450bp. Rest is UTR.Two EcoR1 sites in 3'UTR.



XB-CLONE-12441318



XB-CLONE-12441321



XB-CLONE-12441324

vegfa [provisional]



XB-CLONE-4048748

vegt [provisional]

vegt protein [provisional]

brat, xombi, antipodean, tVegT,

Apod

accession number

associated with different

gene

vegt [provisional]



ApodXB-CLONE-4128542

vegt [provisional]



Apod

Clements, D., Friday, R. V. and Woodland, H. R. (1999). Mode of action of VegT in mesoderm and endoderm

formation. Development 126, 4903-4911.The ORF of VegT (Brat of Horb and Thomsen, 1997)

cloned into pSP64T. Linearised with XhoI and with SP6

vegt [provisional]



Apod

Clements, D. and Woodland, H. R. (2003). VegT induces endoderm by a self-limiting mechanism and by changing the competence of cells to respond to TGF- signals. Developmental Biology 258, 454-463.

The DNA binding domain of VegT fused to the Engrailed repressor domain. Cloned into pST64T. Transcripts were

generated by XhoI digestion and SP6 transcription.

vegt [provisional]



Apod


The entire ORF of VegT fused to the Engrailed repressor domain. Cloned into pST64T. Transcripts were

generated by XhoI digestion and SP6 transcription.

vegt [provisional]



Apod


The ORF of VegT fused to Myc tags and cloned into pSP64T. Transcripts may be generated by XhoI

digestion and SP6 transcription.


















F821


142 vegt-a vegf, vegf-a vascular growth factor pCS2plus VegT not needed p laevis Development 122, 4119 - 4129 pCS2+ amp

81 ventx1.2 vent-1, Xvent-1, vent1 pBut2xVent1 pBut2xVent1 not needed p laevis pBut-2 amp RNA for injection

HAR-56 ventx1.2 vent-1, Xvent-1, vent1 TF Vent-1 IMAGE:4440419 trops 12 p tropicalis CS107 amp

HAR-66 ventx2.1 TF Vent-2 IMAGE:4462078 p tropicalis CS107 amp

HAR-159 xom, Xbr-1a Tf Vent-2 TGas046d19 n/a p tropicalis CS107 amp Tgas046D19- not full length

341 ventral TF Vent 2-Xom not needed p laevis PSP64T

342 Ventral TF deltaVent 2-delta Xom not needed p laevis pSP64T

HAR-86 TF VEX-1 TNeu044o05 N/A p tropicalis CS107 amp AL660431

77-SPH wnt1 signalling molecule pT7TS Xwnt-1 pT7TS Xwnt-1 laevis pT7TS amp

76-SPH wnt1 signalling molecule pGEM bluef Xwnt-1 pGEM bluef Xwnt-1 laevis Noordermeer et al NAR 17:11-18 (1989) pGEM blue amp 1.5 kb EcoRI fragment in pGEM Blue

96-KL wnt10b Xwnt-10b, Xwnt10b signalling molecule Wnt-10b Wnt-10b 13C9 no pictures p tropicalis pCS107 Amp 1.8kb from st19-23 library

409 wnt11 signalling pSP64T-dn-Xwnt11 not needed p laevis pSP64TXB amp

410 wnt11 signalling pSP64T-Xwnt11 not needed p laevis pSP64TXB amp

816 wnt11 signalling molecule Wnt11 Wnt11 - R Mayor not yet in xenbase p laevis Ku et al., 1993. Dev 119: 1161-1173. pGEM-7zf+ Amp From Roberto Mayor

817 wnt11 signalling molecule Wnt11 pXwnt11-A-S-464 not yet in xenbase p laevis pGEM-T easy Amp From E Jones via B Afouda

818 wnt11 signalling molecule Wnt11 pXwnt11 Sense-465 not yet in xenbase p laevis pGEM-T easy Amp From E Jones via B Afouda

LJ-49 wnt11 signalling molecule dnwnt11 dnwnt11 not needed p laevis pSP64T Amp

LJ-39 wnt11 signalling molecule not in xenbase wnt11 wnt11 need doing laevis Tételin and Jones (unpublished). pGEMT Amp 3’ UTR region

LJ-40 wnt11 signalling molecule not in xenbase wnt11-R wnt11-R need doing laevis Tételin and Jones (unpublished). pGEMT Amp 5’ UTR region

47-ER signalling molecule not in xenbase xWNT11 pCS2 xWNT11 laevis pCS2+ Amp

76 wnt3a Wnt3A XE1 not needed p laevis McGrew pSP64T Amp RNA for injection



XB-CLONE-12015552

VENT homeobox 1, gene 2

[provisional] homeodomain



XB-CLONE-4463351

vent vent-1 vent 1 xvent 1 - Genbank ID# BG487195 - Full Length

*this clone has been tested and works for in situVENT homeobox 2,

gene 1 [provisional]

Xbr-1a, xvent-2b, vox1, Xvr-11b, xvent-2

XB-CLONE-4458379

trops 13/14/21/24/28/ 32

xvent Vent 2 vent-2 - BG885317 - Uncertain*Tested and works for in situ

ventx2.1 [provisional]





[provisional] vent-2, vent2, xvent2,

xvent-2, XomXB-CLONE-12271745

Ladhur, R., Mohun, T.J., Smith, J. C. and Snape, A.M. (1996). Development 122, 2385-2394



[provisional] vent-2, vent2, xvent2,

xvent-2, XomXB-CLONE-12271754

Ladhur, R., Mohun, T.J., Smith, J. C. and Snape, A.M. (1996). Development 122, 2385-2394

Designed as a control for RNA injections. It contains initiating methionine 5' region but only half the

homeobox.



[provisional]Xvex-1, vex1, ventral

homeobox 1XB-CLONE-4322213

wingless-type MMTV integration

site family, member 1

Xwnt1, Xwnt-1, Xint-1, int1, int-1, wnt-1

XB-CLONE-14573967


bug is resolved



Xwnt1, Xwnt-1, Xint-1, int1, int-1, wnt-1

XB-CLONE-14573965


bug is resolved


site family, member 10B

XB-CLONE-12442490

Use of large-scale expression cloning screens in the Xenopus laevis tadpole to identify gene function.Grammer TC, Liu KJ, Mariani FV, Harland RM.Dev Biol. December 15, 2000; 228 (2): 197-210.



Wnt11-R, Wnt11r, WNT11-related

XB-CLONE-12441154

Hoppler S, Brown JD, Moon RT: Expression of a dominant-negative

Wnt blocks induction of MyoD in Xenopus embryos. Genes Dev

1996, 10:2805–2817.

A dominant-negative construct, lacking the C-terminal portion of the protein, made according to G&D 10:

2805-2817 (1996)




XB-CLONE-12441157




XB-CLONE-8596722




XB-CLONE-12441618




XB-CLONE-12441621




XB-CLONE-12442040

Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through

the canonical Wnt pathway.Tada M, Smith JC.

Development. May 1, 2000; 127 (10): 2227-38. Expression of a dominant-negative Wnt blocks induction

of MyoD in Xenopus embryos.Hoppler S, Brown JD, Moon RT.

Genes Dev. November 1, 1996; 10 (21): 2805-17.







wnt11b [provisional]


site family, member 11B [provisional]

wnt-11, wnt11, Xwnt-11, xwnt11


site family, member 3A

Xwnt-3a, xwnt3a, wnt-3a

secreted growth factor/developmental

regulator XB-CLONE-11536583














LJ-41 wnt4 Xwnt4, Xwnt-4, wnt-4 signalling molecule wnt4 wnt4 not needed p laevis McGrew et al., 1992. pSP64T Amp

AP-37 wnt4 Xwnt4, Xwnt-4, wnt-4 signalling molecule Xwnt4 Xwnt 4 laevis pCS2+ Amp

LJ-42 Xwnt-5A, wnt-5a signalling molecule wnt5A wnt5A not needed p laevis Moon et al., 1993. pSP64T Amp

LJ-43 Xwnt-5A, wnt-5a signalling molecule wnt5A-myc wnt5A-myc not needed p laevis Moon et al., 1993. pSP64T Amp

LJ-32 wnt6 XWnt-6, xWnt6, wnt-6 signalling molecule wnt6 wnt6 need doing laevis Lavery et al., 2008. pCS2+ Amp Full coding region.

LJ-45 wnt7b signalling molecule wnt7b wnt7b not needed p laevis Chang and Hemmati-Brivanlou, 1998. pBSK Amp

145-KL wnt7b signalling molecule xWnt7B xWnt7B no pictures p tropicalis pCS107 Amp GenBank BG354641

87 wnt8a not needed p laevis Genes and Dev. 10:2805-2817,1996 AMP

HAR-145 wnt8a signaling molecule wnt-8 TGas015m15 tops 11 p tropicalis CS107 amp Full length. Accession number AL631116

LJ-46 wnt8a signalling molecule wnt8 wnt8 not needed p laevis Christian et al., 1991. pSP64T Amp

AP-35 wnt8a signalling molecule Xwnt8 Xwnt 8 laevis pSP64T Amp

LJ-33 wnt-9a signalling molecule Xlwnt9a Xlwnt9a need doing laevis Tételin and Jones, 2008 (unpublished). pBSKS Amp Partial coding (822nt).

LJ-35 wnt-9a signalling molecule not in xenbase Xtwnt9a Xtwnt9a need doing tropicalis Tételin and Jones, 2008 (unpublished). pBSKS Amp Partial coding (822nt).

LJ-37 wnt-9a signalling molecule not in xenbase xtwnt9a xtwnt9a need doing tropicalis Garriock et al., 2007. pGEMT Amp Partial ORF (941nt).

LJ-34 wnt9b wnt-9b signalling molecule not in xenbase Xlwnt9b Xlwnt9b need doing laevis Tételin and Jones, 2008 (unpublished). pBSKS Amp Partial coding (432nt).

LJ-38 wnt9b wnt-9b signalling molecule not in xenbase xtwnt9b xtwnt9b need doing tropicalis Garriock et al., 2007. pGEMT Amp Full length ORF (1074nt).

LJ-4 wt1 Wilms tumor 1 xwt1, wt-1 signalling molecule xWnt-1 64TS xWnt-1 64TS not needed p laevis pSP64T Amp

LJ-22 wt1 Wilms tumor 1 xwt1, wt-1 xWt1-pGEM7-s52S0304 no pictures p laevis pGEMT Amp

44-KL xa-1 XA pXA-1 pXA-1 no pictures p laevis Hemmati-Brivanlou et al., Development 110,325-330 pBSK Amp



XB-CLONE-11536504



wnt5a [Predicted: Source is

Human Entrez Gene]




Gene]

XB-CLONE-11536507


Human Entrez Gene]




Gene]

XB-CLONE-11536510



XB-CLONE-11536513



Xwnt7B, Xwnt-7B, wnt-7b

XB-CLONE-11536516



Xwnt7B, Xwnt-7B, wnt-7b

XB-CLONE-12442515

Use of large-scale expression cloning screens in the Xenopus laevis tadpole to identify gene function.

Grammer TC, Liu KJ, Mariani FV, Harland RM.Dev Biol. December 15, 2000; 228 (2): 197-210.



Xwnt8, Xwnt-8, wnt-8, wnt8

Ventral signalling molecule

XB-CLONE-11536606

dnXwnt-8 CS2plus XE47




XB-CLONE-4142566




XB-CLONE-12442036





Human Entrez Gene]




Gene]

XB-CLONE-12442013


Human Entrez Gene]




Gene]


Human Entrez Gene]




Gene]





XB-CLONE-11536580

zinc finger transcription and splicing factor

XB-CLONE-11879898

x Wt1 pGEM 7 s52S0304

anterior and ectodermic-specific

protein

Actin regulatory protein (Wiskott-Aldrich

syndrome protein)XB-CLONE-12442424

full length XA-1.Expressed during stages 12 to 48 in cement gland and head ectoderm.














HAR-199 xarp arp-A Axin related TNeu129j09 n/a p tropicalis CS107 amp

HAR-329 n/a Xiap tropicalis pExpress1 amp Full length BC091027

91-NP xk81a1 structural protein Epidermal Keratin Epidermal Keratin no pictures p laevis Amp

1 -NP TF Not in xenbase XLH box 4 XLH box 4 tropicalis PGEM2 Amp

78-SPH signalling molecule not in xenbase pGEM3Zf Xwnt-5C pGEM3Zf Xwnt-5C laevis pGEM3Z blue amp

79-SPH signalling molecule not in xenbase pT7TS Xwnt-5C pT7TS Xwnt-5C no pictures p laevis pT7TS amp

908 yipf3 finger3, klip1 Golgi maintenance Klip1 Klip1 (7391210) no pictures p laevis pExpress1 Amp

LJ-112 zeb2 not in Xenbase SIP1 SIP1 laevis Masse et al. in press, IJDB. pBSKS amp 3' UTR; 1kb; 3145-4106

1099-AP zic1 TF OpiI laevis

HAR-28 zic2 TF Zic2 TGas015e14 trops 15-16-19 -25-28 p tropicalis CS107 amp Zic2 zic2 zic Zic - 3' present - Al631793

152-NP zic2-a TF pBS Zic 2 Probe pBS Zic 2 Probe n/a p laevis pBS Amp

867 ehzf, evi3 TF Evi3 IMAGE:6316008 no pictures p laevis pCMV-SPORT6 Amp

HAR-427 n/a miR-1a1 microRNA miR-1 no accesion number tropicalis PCRII-TOPO ampHAR-429 n/a miR-9a-1 microRNA miR-9 no accesion number tropicalis pOCRII-TOP ampHAR-431 n/a miR-9-3 microRNA miR-9 tropicalis pOCRII-TOP ampHAR-432 n/a miR-10c microRNA miR-10 tropicalis pOCRII-TOP ampHAR-433 n/a miR-18a microRNA miR-18 tropicalis pOCRII-TOP ampHAR-437 n/a miR-98 microRNA miR-98 tropicalis pOCRII-TOP ampHAR-440 n/a miR-133b microRNA miR-133 tropicalis CS107 amp

Axin-related protein [Predicted:

Source is Laevis Entrez Gene]


XB-CLONE-4235567

The first few nucleotides may be missing in this clone - just short of full length. Accession number AL802722

xiap [Predicted: Source is

Human Entrez Gene]

X-linked inhibitor of apoptosis


Gene]

api3, ilp1, miha, xlp2, birc4, xxiap

Apoptosis inhibitor IAP1 and related BIdomain

proteins

Keratin, type I cytoskeletal 47

kDa

epidermal keratin, epi-keratin, epiker,

cytokeratin, Epidermis-specific keratin, xK81,

EpK

XB-CLONE-12442338

XlHbox4 not in Xenbase http://www.ncbi.nlm.nih.gov/nucleotide/64755?report=genbank&log$=nuclalign&blast_rank=1&RID=J6VB9BKH01R

xwnt-5c not in xenbase


bug is resolved

xwnt-5c not in xenbase http://www.ncbi.nlm.nih.gov/pubmed/8202371

Analysis of Wnt/Engrailed Signaling in XenopusEmbryos Using Biolistics. Johanna G. Koster, et. al. DEVELOPMENTAL BIOLOGY 173, 348–352 (1996)

Article No. 0029

Yip1 domain family, member 3 [Predicted: Source is Human Entrez

Gene]

XB-CLONE-1706742

Image EST 7391210, Accession BC124836. Also known as YIPF3

zinc finger E-box binding homeobox

2

zfhx1b, smad interaction protein 1,

XSip1, sip1G-protein coupled

receptor

Zic family member 1

Zic-r1, opl, odd-paired-like, Zinc finger protein

of the cerebellum 1, Zic-related-1

Zic family member 2

Zinc finger protein of the cerebellum 2,

xzic2, hpe5XB-CLONE-4135258

Zinc finger protein of the cerebellum 2,

xzic2, hpe5XB-CLONE-12442356

znf521 [Predicted: Source is

Human Entrez Gene]

zinc finger protein 521 [Predicted:


XB-CLONE-2190599

Image EST 6316008, Accession BC068658. Also known as Znf521














Expression pattern Original Author Insert size and sites Antisense Linearisation Antisense Polymerase Antisense size Sense Linearisation Sense Polymerase Sense size Purpose Organism

PvuII SP6 To generate probe for in situ.

EcoRI SP6 To generate mRNA for injection Xenopus laevis


SmaI SP6 To generate mRNA for injection Xenopus laevis


Full length, NotI-SalI SmaI or EcoRI T7 To generate probe for in situ Xenopus

BamHI T7 NotI SP6 Injection + WISH X. laevis

Xenopus tropicalis

BamHI T7 Asc SP6 Xenopus tropicalis

A. Zorn ~2.2 kb BamHI T7 Xenopus tropicalis

kpnI T7 Xenopus tropicalis

cement gland NotI SP6

Haematopoietic Full length, NotI-SalI SmaI T7 To generate probe for in situ Xenopus

NotI-SalI SmaI or EcoRI T7 To generate probe for in situ Xenopus

T. Pieler lab BamHI T3 NotI SP6 IN SITU AND INJECTIONS Xenopus

SpeI T7

Somite - Brain, DA NotI-SalI SmaI-EcoRI T7 To generate probe for in situ Xenopus

ClaI/XhoI BamHI T7 NotI SP6 To generate probe for in situ and mRNA for injection.


Full length, EcoRV-NotI SmaI T7 To generate probe for in situ Xenopus

Full length, NotI-SalI T7 To generate probe for in situ Xenopus

EcoRI T7 Xenopus tropicalis

NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

Neural - Rohon Beard neurons

Invitrogen / IMAGE Clone / XGC clone

Blood - Embryonic haemangioblast, VBI,

Myeloid cells

Mesoderm - Eye - dorsal lip, paraxial mesoderm, lateral

plate, ventral mesoderm,

mesonephros

Hypochord, brain, BA, Heart


Aaron Zorn 2285bp RI T7 Xenopus tropicalis

To generate mRNA for injections.

T3?

KpnI T7 Xenopus tropicalis

Pronephros Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

Aaron Zorn ~1.5 kb ClaI T7 Xenopus tropicalis

Aaron Zorn 1.2 kb ClaI T7 Xenopus tropicalis

ClaI SP6 Xenopus tropicalis

Xenopus tropicalis

BamHI T7 NotI SP6


AscI SP6 Xenopus tropicalis



Full length, NotI-SalI RsrII T7 To generate probe for in situ Xenopus

XbaI SP6 To generate mRNA for injection

Les Dale BamH1 T3 In situ hybridization Xenopus

Aaron Zorn 1 kb ClaI T7 Xenopus tropicalis

Aaron Zorn ~2.5 kb ClaI T7 Xenopus tropicalis

Mike Jones EcoRI SP6 In situ hybridization Xenopus

Aaron Zorn ClaI T7 Xenopus tropicalis

ClaI T7 Xenopus tropicalis

Brain, BA, cement gland, nueral tube,

HSC

1.04 kb BamH1-EcoRI fragment of XBMP-2

900 bp EcoRI-SphI fragment




1.67kb XbaI sp6 In vitro RNA synthesis

KpnI T7 NotI SP6 Xenopus tropicalis

Andres 2kb T7 Kpn SP6 Xenopus tropicalis


IMAGE BamH1 T7 Xenopus tropicalis

NotI SP6

BamHI SP6

Ken Cho Sac 1 SP6 To generate mRNA for injection Xenopus


Wright lab 3.3kb EcoRI-Xba EcoRI T7 NotI SP6 in situ probe and mRNA for injection Xenopus

ClaI T7 NotI SP6

Xenopus tropicalis

EcoRI T7 AscI Xenopus tropicalis

Apoptosis Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

PGC M.L. King EcoRI SP6 In situ hybridization Xenopus

Full length, Not-SalI SalI or EcoRI or EcoRV T7 To generate probe for in situ Xenopus

SalI-NotI SmaI T7 To generate probe for in situ Xenopus

Somites, PN, PD, brain, neural tube, BA,

Blood - VBI, Somites, brain


SalI-NotI SalI or SmaI T7 To generate probe for in situ Xenopus


EcoR1 -XhoI T7 sp6XbaI - XhoI T7 sp6ClaI - XbaI T7 sp6

BamHI - ClaI T7 sp6

BamHI - ClaI T7 sp6 To generate mRNA for Injection and probe for in situ.

KpnI T7

EcoRI - XhoI T7 sp6 To generate mRNA for injection.

NotI-SalI SalI or SmaI T7 To generate probe for in situ Xenopus

William Bement NotI/Sp6

?

Full length, NotI-SalI SalI or SmaI T7 To generate probe for in situ Xenopus

XbaI - XhoI T7 sp6

879bp BamHI - XbaI T7 sp6 To generate probe for in situ and mRNA for injection. Xenopus laevis

To generate mRNA for injection.

ClaI - XhoI T7 sp6 To generate probe for in situ and mRNA for injection.

EcoRI T7


Brian Mitchell NotI/SP6 C-term RFP fusion5' EcoRI / 3 'XbaI cloning

NotI SP6

NotI SP6

1.4KB EcoRI-XhoI NotI T7 XhoI T3

Not 1 SP6 blocks nodal signalling xenopus

Aaron Zorn ~1-2kb BamH T7 Xenopus tropicalis

Aaron Zorn ~1.5-2kb BamH1 T7 Xenopus tropicalis

AscI SP6 To generate mRNA for injection Xenopus laevis

EcoRV-NotI EcoRV or SmaI or EcoRI T7 To generate probe for in situ Xenopus

Blood - VBI, Somites, brain

Blood - Neural Crest - VBI, HSC, brain

Blood - Blood vessels, DA

wGBD fused to eGFP for visualizing active

Cdc42 in vivo

Centrin2Unigene ID: Xl.50473

Endoderm - Blood - Endothelial cells, blood

vessels


Aaron Zorn 2-3 kb To generate probe for in situ. Xenopus tropicalis

Aaron Zorn 3 kb Ecor1 T7 Xenopus tropicalis

Aaron Zorn 1 kb Ecor1 T7 Xenopus tropicalis

Aaron Zorn 2-3 kb Ecor1 T7 Xenopus tropicalis

Aaron Zorn 2.5-3 kb EcoR1 T7 Xenopus tropicalis

Aaron Zorn ~2 kb BamH1 T7 To generate probe for in situ. Xenopus tropicalis

Aaron Zorn ~3 kb EcoR1 T7 To generate probe for in situ. Xenopus tropicalis

Aaron Zorn 2-3 kb EcoR1 T7 To generate probe for in situ. Xenopus tropicalis

Aaron Zorn 2-3 kb EcoR1 T7 To generate probe for in situ. Xenopus tropicalis

NotI SP6

2.8KB EcoRI T7 WISH

NotI SP6 To generate mRNA for injection Xenopus laevis

To generate probe for in situ and mRNA for injection.

PCR T3/T7 T7

Liz Jones Not1 Sp6 To generate mRNA for injections Xenopus laevis

NotI-SalI SmaI T7 To generate probe for in situ Xenopus

AgeI T7 Xenopus tropicalis

IMAGE Xenopus tropicalis

Blood - VBI NotI-SalI SalI T7 To generate probe for in situ Xenopus

ClaI T7 SP6 Xenopus tropicalis

Xenopus tropicalis

IMAGE EcoRI T7 AscI SP6 Xenopus tropicalis

Full length, NotI-SalI SmaI T7 To generate probe for in situ Xenopus

Xenopus tropicalis

Kimelman, Moon Lab NotI SP6 Capped RNA ?

? ?

Head Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

Moepps&Gierschik 1161bp EcoRV-ApaI NotI T7 To generate probe for in situ Xenopus


2.4kb EcoRI 5' NotI 3' CTGF IMAGE 7803217

Neural Crest - Eye - neural crest, otic vesicle, forebrain, hindbrain, eyes,

branchial arches, body wall


546bp EcoRI-BamHI EcoRI SP6 To generate probe for in situ. Xenopus

SalI-NotI SmaI T7 To generate probe for in situ Xenopus

Full length, ClaI-XbaI NotI SP6 RNA for injection Xenopus

Xenopus tropicalis

A. Zorn / Karen Liu Cla1 T7 Asc1 Sp6 To generate probe for in situ and mRNA for injection. Xenopus tropicalis

1kb SalI/NotI SalI T7 AscI Sp6

PGC M.L. King SmaI T7 In situ hybridization Xenopus

Myeloid Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

PGC M.L. King HindIII or XbaI T7 In situ hybridization Xenopus

2.4kb SalI T7 To generate probe for in situ Xenopus

Xba 1 SP6 1.3Kb injection Xenopus

HindIII T7 1.3Kb in situ Xenopus

Aaron Zorn 1.5 kb T7 AscI SP6 To generate probe for in situ and mRNA for injection. Xenopus tropicalis

1.5kB SP6 To generate mRNA for injection

HindIII - EcoRI T7 sp6

EcoRI-XhoI EcoRI or ClaI or BamHI T7 To generate probe for in situ Tropicalis

Xenopus tropicalis

A. Zorn Xenopus tropicalis

Xenopus tropicalis

A. Zorn Cla1 T7 Xenopus tropicalis

Xenopus tropicalis

Not1 T3 To generate probe for in situ.

Xenopus tropicalis

Somite - Neural - Marginal zone, somites,

tailbud, neural tube, forebrain, cement

gland

De Ross From Durston's lab

Somites, blood vessels, heart, DA

R1* BamHI and Hind III ok too

Vasculature - Arterial specific

EcoRI - NotI 300 bp band


EcoRI T7

To generate mRNA for injection Xenopus laevis


Not1 SP6 RNA injection xenopus

Not1 SP6 To generate mRNA for injection xenopus

Not1 SP6 To generate mRNA for injection Xenopus

Not1 SP6 To generate mRNA for injection Xenopus


NotI/ SP6

NotI/ SP6

NotI/SP6

HindIII

NotI T3

NotI Sp6

NotI T7

R. Fletcher Xenopus tropicalis

Asp718 T7 Xenopus tropicalis

wikinson lab XbaI T3 Xenopus Laevis

Xenopus tropicalis


Wilkinson EcoR1(long version) T7 (long version) Whole mount in situ hybridisation Xenopus

Davis Wikinson 509bp eco-Pst EcoRi T3 HindIII/PstI T7 Xenopus

EcoRI T7

amino acids 1-374 of xenopus

dishevelledcloning site EcoR1-Xho1

Dishevelled (Xenopus) in EcoR1-Xho1amino acids 1-301 and 381-

736EcoR1-Xho1gene

fragment - amino acids 178-736

EcoR1-Xho1 gene fragment - amino acids

337-736

Ute Rothbacher Scott Fraser

Xdsh2190 BfaI fragment delete 707-

990

for expression of disheveled mRNAPDZ deletionaxis-inducing membrane translocating phosphorylatedCHECK

ORIGINAL DETAILED INFO IN PLASMID BINDER


Xdsh2190 BfaI fragment

for expression of disheveled mRNAMissing most of DEP and C-terminal GFP taggedaxis-inducing YES membrane

translocating NO phosphorylated NOCHECK ORIGINAL DETAILED INFO IN PLASMID BINDER



for expression of disheveled mRNAamplified 5'TGCCGTGCTTTAATGG/ACCG5'GGTCCCAGCATTTGGCCAC fragment used to screen cDNA librariesHA tag near N-

terminusaxis-inducing membrane translocating phosphorylatedCHECK ORIGINAL DETAILED INFO IN

PLASMID BINDER

insert cloned into sal I-Not I

Xenopus cDNA for in situ or RNASe protextion probe for Krox-20.


900bp Sal1 T7 Sal1 SP6 in situ probe xenopus

3.6 kb Sal1 T7 Sal1 SP6 in situ probe xenopus

Sal 1 T7 Sal 1SP6 in situ probe xenopus

Blood - Blood vessels Full length, NotI-SalI SalI or SmaI T7 To generate probe for in situ Xenopus

Full length, NotI-SalI SalI or SmaI T7 To generate probe for in istu Xenopus

Blood - Myeloid Full length, EcoRV-NotI EcoRV or SmaI T7 To generate probe for in situ Xenopus laevis



XbaI T3

Xenopus laevis

Xenopus laevis

Xenopus laevis

J. B. gurdon SfiI T3 RNA injection Xenopus

Kenneth Ryan 2.9kb EcoRI-NotI EcoRI T3 NotI T7 To generate Eomesodermin probe for in situ Xenopus

Aaron Zorn ~3kb Xenopus tropicalis

Aaron Zorn ~3kb RI T7 Xenopus tropicalis

Aaron Zorn ~3-4kb EcoR1 T7 In situ Hybridization Xenopus tropicalis

wikinson lab 1.8kb truncated EphA4 XbaI SP6 Xenopus Laevis

2.6KB HIndIII T7


Aaron Zorn Not full length ClaI T7 Xenopus tropicalis

EcoRI T7NotI SP6

1.1kb, EcoRV XbaI T7 To generate mRNA for injection Xenopus

XbaI-SmaI SmaI or EcoRI or RsrII T7 To generate probe for in situ Xenopus

Eye - Neural Crest - brain



Mesoderm - DA clusters, lateral plate

mesoderm

Eye - Neural Crest - mesoderm

2.2 kb not full length

Endoderm - Endothelium, lymphatics

somites, head, BA, PN, PD, neural tube, HSC,

vessels


300bp DdeI T7 In situ Probe

andrea EcoRI Xenopus tropicalis

HindIII/EcoRI

EcoRI/XhoI

SalI Xenopus tropicalis

SacI T7 Xenopus tropicalis

BamHI T7 AscI SP6 To generate probe for in situ and mRNA for injection. Xenopus tropicalis

Weiyang Shi NcoI SP6 Xenopus tropicalis

NcoI SP6 Xenopus tropicalis



EcoRI T7 AscI SP6 Xenopus tropicalis

SacI/SacII Xenopus tropicalis

BamHI Xenopus tropicalis

IMAGE SacII SP6 Xenopus tropicalis

SacII T7

Issacs SacI sp6 in vitro transcription of eFGF RNA

Issacs SacI sp6 in vitro synthesis of a/s eFGF RNA

EcoRI T7 AscI SP6 To generate probe for in situ and mRNA for injection. Xenopus tropicalis

A. Zorn ~2.5 kb insert Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

constructed by James Howard - published by Harry Isaacs - supplied

by Hoppler Lab


SacI T7 Xenopus tropicalis

A. Zorn ~3.8 kb Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

BamHI T7 In Situ Probe Xenopus tropicalis





Xenopus tropicalis


Xenopus tropicalis


Xenopus tropicalis

Xenopus tropicalis

EcoRI T7 In Situ Probe Xenopus tropicalis

EcoRI T7 In Situ Probe Xenopus tropicalis

SmaI T3 WISH Xenopus

Full length, NotI-SalI SmaI or EcoRI or ClaI T7 To generate probe for in situ Xenopus

Xenopus tropicalis

NotI T7 KpnI T3 In situ Probe X. laevis

NotI T7 KpnI T3 In situ Probe X. laevis

Endothelial cells Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

Endothelial cells Full length, NotI-SalI SalI or SmaI T7 To generate probe for in situ Xenopus


NotI-SalI SalI T7 To generate probe for in situ Xenopus


Endoderm - Endothelium, neural

crest1.371 kb (from

nucleotide 1 to 1371)

Vasculature - Blood vessels

Sally Moody / Marli Dirksen


Endoderm - Endothelial cells


A. Zorn RI T7 Xenopus tropicalis


Maz O'Reilly 840bp pstI-XbaI frag. Bgl2 or BamH1 T3 385 or 620 295 or 530 RNase protection and in situs Xenopus

BamHI T7 Xenopus tropicalis


Full length, EcoRV-NotI EcoRI T7 To generate probe for in situ Xenopus

Aaron Zorn Xenopus tropicalis

Xenopus tropicalis


EcoRI Xenopus tropicalis

James McGann Xenopus tropicalis

EcoRV-NotI SmaI or EcoRI T7 To generate probe for in situ Xenopus

SalI-NotI AscI SP6 Xenopus Laevis

Xenopus tropicalis

Invitrogen SalI T7 AscI SP6 Xenopus tropicalis


Aaron Zorn claI T7 Xenopus tropicalis

1.8 kb XbaI/BamHI T7 XhoI T3 In situ Probe X. laevis

Xenopus tropicalis

Xenopus tropicalis

BamHI T7 Xenopus tropicalis



Invitogen Xenopus tropicalis


BamH1 T7 In situ Hybridization Xenopus tropicalis

Somites, tailbud, vitelline vessels

Somites, tailbud, vitelline vessels

Sally Moody / S. Sullivan


Aaron Zorn ~1-2 kb insert EcoR1 T7 Xenopus tropicalis

Xenopus tropicalis



SalI T7



EcoR1 T7 in situ Xenopus

IMAGE HindIII or R1 T7 Xenopus tropicalis

A. Zorn ~2kb insert Xenopus tropicalis

A. Zorn ~9.6 kb insert? Xenopus tropicalis

NotI SP6

HindIII T7 NotI SP6

Crescent 2.9Kb Kpn1 SP6 Xho1 T7 Xenopus

Hind III T7 Not1 SP6 xenopus

NotI SP6 To generate RNA for Injection

Liz Jones



XbaI SP6 To generate mRNA for injection Xenopus laevis

ClaI T7 AscI Xenopus tropicalis

Randal Moon BamHI-XbaI XbaI SP6 BamHI T7 Capped RNA & In situ Probe

1.9 kb SacI-BamHI In situ Probe

Capped RNA

4.5Kb HindII T7 NotI T3 To generate probe for in situ and Capped RNA.

1.8kb NotI SP6 Injection

3.3kb EcoRI T7 NotI T3 Capped RNA & In situ Probe


VBI. EcoRI 1.85kb XhoI SP6 2kb wish probe, a/s for Xenopus GATA1a Xenopus

Walmsley et al 1.3Kb, EcoR1 Xba1 sp6 1.3Kb Hind111 T7 1.3Kb Whole mount in situ hybridisation Xenopus

~1.5 kb(?) cuts in insert with EcorI/NotI

Grant Wheeler - Hoppler Lab

Grant Wheeler (Hoppler Lab)

constructed by Matt Deardorff - Klein Lab -

Hoppler Lab

constructed by Leslee Conrad, Klein Lab,

Hoppler Lab



Alison Brewer EcoR1 Sp6 Xenopus GATA2 RNA overexpression Xenopus

- - - - Eco RI T3 2.5 kb In vitro transcrption template for injection RNA Xenopus laevis

Carole RNA for injection Xenopus

Mary EcoRI T3 EcoR1 T3 for RNA to overexpress GATA3 Xenopus

heart, endoderm Jiang and Evans 1996 1.6 kb EcoRI XhoI or HindIII T3 Full-length in situ probe Xenopus

heart, endoderm EcoRI, EcoRV SmaI T7 in vitro RNA synthesis Xenopus

Sal1 SP6 RNA injection xenopus

EcoRI T3 ~1.18Kb To generate mRNA for injection. xenopus

SfiI T3 ~1.18Kb To generate mRNA for injection. xenopus

Sfi1 T3 ~1.18Kb To generate mRNA for injection xenopus

Xenopus tropicalis

Xenopus tropicalis

Adam ca 1.2 kb XhoI /XbaI EcorI T3 polymerase in vitro transcription of Xenopus GATA5amyc RNA

- - - T3 2.35 Kb Injection RNA template for Gata5 full length engrailed Xenopus

heat, endoderm Jiang and Evans 1996 1.9 kb EcoRI SmaI T7 Full length in situ probe Xenopus

Sal1 SP6 RNA injection xenopus

EcoRI T3 ~1.160Kb To generate mRNA for injection. xenopus

EcoR1 T3 ~1.2Kb RNA injection Xenopus

Sfi1 T3 ~1.2Kb RNA injection Xenopus

Heart, VBI NotI-SalI SalI or SmaI T7 To generate probe for in situ Xenopus

Todd Evans 4-5kb SacI SP6 in vitro transcription of Xenopus GATA5 RNA a/s

Xenopus tropicalis

Same as full length Gove 578bp XbaI-EcoRI In situ probe Xenopus

Maz O'Reilly EcoRI T3 2.4kb RNA for injection Xenopus

Xba 1 Xho 1 sites EcoR1 T3 1.3Kb injection Xenopus

~1.3kb BamH1 fragment inserted into

BglII

1360bp PCR product into XbaI XhoI (5'-3')

Gata2 fingers, Xho1/Xba1

SpeI 443 to HincII2349 into XbaI/HincII

Cloned into BglII site of pSp64T-GR-HA

Cloned into XbaI and XhoI sites of pBut2-HA

Cloned into XbaI and XhoI sites of pBut3-HA

Cloned into Xba1 and Xho1 sites of pBut3-

HAThis construct contains the full 5' UTR

(not present in plasmid number 371)

1.2 into Xba I Xho I 5'-3'

(Pst 1) This should be Eco R1 - see Martin's

project report.

Cloned into BglII site of pSp64T-GR-HA

Cloned into XbaI and XhoI of pBut2-HA

Cloned into Xba1 and Xho1 sites of pBut2HA. This construct contains sequences upstream of the ATG not present in

XGATA5-HA-pBut2

Cloned into Xba1 and Xho1 sites of pBut3-HA. This construct contains sequences upstream of the ATG not present in

XGATA5-HA-pBut3

GATA6 (full-length short form) amplified and inserted into XbaI

and XhoI sites of BUT2-EnMT.



SalI SP6 To generate mRNA for injection xenopus


Xenopus tropicalis

Xenopus tropicalis

To generate probe for in situ and mRNA for injection. Xenopus tropicalis

Sfi1 T3 RNA for injection Xenopus

Gove et al. 3.3Kb, EcoR1 Not1 T7 3.3Kb Sal1 T3 3.3Kb Whole mount in situ hybridisation Xenopus

A. Zorn 0.85 kb insert??? Xenopus tropicalis

A. Zorn ~3 kb insert? Xenopus tropicalis

Xenopus tropicalis

Cement gland, skin Full length, SalI-NotI SalI or SmaI T7 To generate probe for in situ Xenopus


Benjamin Sun 1.5 kb full-length BamHI T3 NotI SP6 Injections Xenopus

EcoR1 T7 Not1 SP6 in situ and RNA injection xenopus

A. Zorn R1 T7 Xenopus tropicalis

C Chang NotI T7 To generate probes for in situ Xenopus

BamHI T7 Asc SP6 Xenopus tropicalis

SmaI-NotI EcoRI or ClaI T7 To generate probe for in situ Xenopus

Blood - VBI Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

NotI-SalI SalI-SmaI T7 To generate probe for in situ Xenopus

Gills, head Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

SalI-NotI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

Blood - DA, arteries Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus


Ectoderm, nerves Full length, NotI-SalI SmaI T7 To generate probe for in situ Xenopus

Epidermis - Head, eyes T takabatake 1.8kb BamHI-XbaI EcoRI T3 To generate probe for in situ Xenopus

Insert size is ~1600bp. Cloned into BglII site of

pSp64T-GR-HA

Ventro - lateral mesoderm in neurula

Heart and blood in tailbud Heart and

endodermal derivatives (foregut) in adult

Neural plate, hind brain, eyes

Blood - DA, myeloid, VBI

Endothelium, blood vessels, somites

Skeletal Muscle, somites, heart, body

wall



st 34 RNA 1464 EcoRV ApaI SP6 NotI T7 To generate probe for in situ and mRNA for injection.

Epidermis T takabatake 751bp BamHI-EcoRI BamHI SP6 To generate probe for in situ Xenopus

Katia Koebernict XbaI 4747bp BamHI T3 NotI SP6 To generate probe for in situ and mRNA for injection.

Katia Koebernict XhoI 4081bp NotI SP6 To generate mRNA for injection.


EcoRI T3

Xenopus tropicalis

Blood - Myeloid NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

Platelet Full length, NotI-SalI EcoRI T7 To generate probe for in situ Xenopus


BamHI T7 NotI SP6

Hugh Woodland Not1 SP6 RNA Injection laevis

Aaron Zorn 1.5 kb (uncertain) Xenopus tropicalis

Aaron Zorn BamH1 T7 Xenopus tropicalis

Aaron Zorn 1-1.5 kb Xenopus tropicalis

Aaron Zorn ~1 kb Xenopus tropicalis


Aaron Zorn ~<1 kb Xenopus tropicalis

XbaI SP6

525 BamHI T3 T7 To generate probe for in situ and mRNA for injection.

David Kimelman NotI SP6 RNA injection

Xenopus tropicalis

Xenopus tropicalis

PCR T3/T7 T7

Brain, eyes, otic vesicle, pronephros and duct, late VBI


EcoRI T7 NotI SP6

Xenopus laevis

BamHI T7

neural crest WISH xenopus




Invitrogen EcoRI T7 Xenopus tropicalis

Mike jones Bam H1 T7 in situ probe Xenopus


A. Zorn BamH1 T7 Xenopus tropicalis

SP6 Not 1

Asp718 or SmaI T7 XhoI SP6 in situ probe and mRNA for injection Xenopus


EcoRV-NotI SmaI T7 To generate probe for in situ Xenopus

1.4kb EcoRI T7 Xenopus tropicalis

Xenopus tropicalis

EcoRI T7

Aaron Zorn ~1 kb EcoR1 T7 Xenopus tropicalis


Xenopus tropicalis

Xenopus tropicalis


A. Zorn T7 Asc1 Sp6 To generate probe for in situ and mRNA for injection. Xenopus tropicalis

Histone-2B - fused in frame to eGFP

live cell nuclear marker. Cloned blunt into XhoI/XbaI sites of CS2

Rhombencephalon, Branchial arch4


R1 T7 ASC1 S Xenopus tropicalis


Xenopus tropicalis

HIndIII T7 AscI Sp6 To generate probe for in situ and mRNA for injection.

James McGann Xenopus tropicalis

NotI-EcoRI EcoRI or BglII T7 To generate probe for in situ Xenopus

T7 AscI Sp6 To generate probe for in situ and mRNA for injection.

462bp NotI sp6

EcoRI T7 NotI SP6

EcoRI T7 NotI SP6

NotI sp6 EcoRI T7

2.6kb BamHI T3 To generate probe for in situ Xenopus

Xenopus tropicalis

bgl11 EcoR1 fragment Sst1 SP6 To generate mRNA for injection xenopus


EcoRI sp6


PCR T3/T7 T7

EcoRI T7

Gomez-Skarmeta XbaI T7 In situ Probe


IMAGE EcoRI T7 Xenopus tropicalis

Xenopus tropicalis

Gomez-Skarmeta NcoI SP6 In situ Probe

Cement gland, somites, VBI, head, HSC


Gomez-Skarmeta XbaI T7 In situ Probe

Gomez-Skarmeta BamHI T7

NotI-EcoRI EcoRI T7 To generate probe for in situ Xenopus


Xenopus tropicalis

Grant Wheeler EcoRI/XhoI EcoRI T7 XhoI T3 To generate probe for in situ and mRNA for injection. Xenopus laevis

EcoRI T3

HindIII T7 NotI SP6


Full length, NotI-SalI EcoRI T7 To generate probe for in situ Xenopus


Xenopus tropicalis

PCR T3/T7 T7 WISH

Xenopus tropicalis

c.Baker EcoR1 T3 WISH Xenopus

AgeI T7 Xenopus tropicalis


DA NotI-SalI RsrII or KpnI T7 To generate probe for in situ Xenopus


Primary neurons, secondary heart field

1) We confirm that this clone is probably in the pCS105 vector.2) Cutting with StuI releases a 500bp fragment-it does not linearize. There is a StuI site at ~779 and at ~1790 (see attached map) in the ORF. 3) We found that you also can not cut with HindIII; the digest releases a 1.3kb insert4) We used EcoRI to cut at the 5' end for antisense RNA; you also can cut with SalI (predicted to get a ~2kb product with either EcoRI or SalI)5) It is well-known that the pCS105 T7 site is mutated and inactive; therefore T3 must be used.6) By using EcoRI and T3, we obtained a very nice probe that stains as reported in the literature.

Brain, neural tube, eyes, PN, fin, heart

Blood vessels and myeloid cells



Blood - Myeloid cells SalI-NotI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

Liz Jones Sfi1 T3 To generate mRNA for injections

Xba1 SP6 to generate mRNA for injections xenopus

full length Xba1 SP6 to generate mRNA for injections xenopus

Oliver Destree

Aaron Zorn Full Length Xenopus tropicalis

HindIII T7 NotI SP6 Injection + WISH X. laevis

Xenopus tropicalis

Xenopus tropicalis

BamHI SP6 XhoI T7

Taira 3kb, EcoR1 Xho1 T7 BamH1 T3 Xlim-1 WISH Xenopus

Xba1 T7 xenopus


Liz Jones Xba1 sp6 To generate mRNA for injections

Liz Jones Xho1 T7 To generate probe for In situ

Taira 957bp EcoRI BamH1 T3 HindIII T7 Xlim-3 WISH Xenopus

Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus laevis

SalI-NotI SalI or EcoRV T7 To generate probe for in situ Xenopus

Liz Jones ?

Liz Jones Sst2 SP6 To generate mRNA for injections

Heart Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus


sp6? in vitro RNA synthesis. Xenopus

Blood - VBI, brain Shoko Ishibashi BamHI T7 in situ Xenopus

Shoko Ishibashi ~950bp EcoRI-XhoI NotI SP6 To generate MafB mRNA for injections Xenopus

H Shibuya 2.5kb SalI-XhoI EcoRI T7 ? T3 To generate mRNA for injections and in situ probe Xenopus

acknowledge Stefan Hoppler

acknowledge Stefan Hoppler

Kidney - Brain and pronephros and duct

Blood - Endoderm - VBI, endothelium,

endocardium, HSC, brain

Heart, presomitic mesoderm,

pronephros, neural tube, head

660 bp RT-PCR product in BamHI/EcoRI site



H Shibuya 1.8kb XhoI-XbaI NotI or KpnI SP6 To generate mRNA for dominant negative form of TAK1 Xenopus

Stu1 500bp Not1 SP6


Full length, NotI-SalI SalI or EcoRI T7 To generate probe for in situ Xenopus

Aaron Zorn ~1.5-2kb BamH T7 Xenopus tropicalis

Aaron Zorn ~2-3kb EcoR1 T7 Xenopus tropicalis

Aaron Zorn ~2 kb BamHIm(not EcoRI) T7 In situ Hybridization Xenopus tropicalis

NotI-EcoRI EcoRI or BamHI T3 To generate probe for in situ Xenopus

Patrick Lemaire SfiI T3 To generate mRNA for injection. Xenopus laevis

Sal1 SP6 xenopus


1450 Asc1 sp6 in situ Xenopus

EcoRI T7 NotI Xenopus tropicalis


Blood - Myeloid NotI-SalI SalI or SmaI T7 To generate probe fon in situ Xenopus

NotI T3

colin sharpe EcoRI ~ 1.4 notI T7 To generate probe for in situ.

Xenopus tropicalis

A Suzuki 1.8kb, EcoRV EcoRI SP6 To generate mRNA for injections Xenopus

sp6 in vitro RNA synthesis

Michael King Full length NotI BbvCI or BbsI T7 SfiI or KpnI T3 To generate probe for in situ or mRNA for injections Xenopus

Heart NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

A. Zorn BamHI T7 Xenopus tropicalis

Aaron Zorn BamHI T7 Xenopus tropicalis

somites, muscle NotI-SalI HindIII or BamHI or ClaI T7 To generate probe for in situ Xenopus

XbaI sp6 To generate mRNA for injection

Toby Sykes RPA

Aaron Zorn 0.5 kb BamHI T7 Xenopus tropicalis

Aaron Zorn ~1-2kb BamHI T7 Xenopus tropicalis

Aaron Zorn ~1.6-2 kb BamHI T7 Xenopus tropicalis

Cla1 T7 Xenopus tropicalis

Aaron Zorn ~1-2kb BamH1 T7 To generate probe for in situ. Xenopus tropicalis

Newly differentiated and differentiating

somites

necessary to amplify pcr fragment



1434bp HindIII T7 XbaI T3 To generate probe for in situ and mRNA for injection.

NcoI - BalI/BstE II XbaI sp6 To generate mRNA for injection.

To generate probe for in situ Xenopus

EcoRI T7 NotI SP6

ClaI Sp6

Neural Plate HindIII SP6 Whole mount in situ hybridisation Xenopus

Blood - Myeloid Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

Blood - Myeloid Full length, NotI-SalI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Jacqueline Lee 1.4 kb To generate mRNA for injection



BamH1 T3

NotI T7 Xenopus tropicalis

Full length, NotI-SalI T7 To generate probe for in situ Xenopus


Hugh Woodland

EcoRI T7 AscI SP6 Xenopus tropicalis

Alice Watson 752bp NotI SP6 Xenopus tropicalis

Heart EcoRV-NotI SmaI or EcoRI T7 To generate probe for in situ Xenopus

1.9kb ish hind3 T7 WISH Xenopus

Mustafa 600 bp PCR product BamH1 T7 Xenopus tropicalis

Xenopus tropicalis

Chris Kintner, Salk Inst (601) 485 6675



1930 Xba T7 To generate probe for in situ.

Heart PstI-XhoI SP6 To generate probe for in situ Tropicalis




Xenopus tropicalis

Aaron Zorn ~2kb EcoR1 T7 Xenopus tropicalis

Mike Jones SmaI T7 In situ hybridization Xenopus

Hugh Woodland

Mike Jones SmaI T7 In situ hybridization Xenopus

Mike Jones EcoRI T7 NotI SP6 In situ hybridization / injections Xenopus

Aaron Zorn Cla1 T7 Xenopus tropicalis

EcoRI T7 SP6 Xenopus tropicalis

Endoderm, Mesoderm NotI, SacI T7 XhoI T3 RNA injection and in situ probe Xenopus

Endoderm, Mesoderm NotI, SacI T7 XhoI T3 RNA injection and in situ probe Xenopus


Xenopus tropicalis

Xenopus tropicalis


KpnI SP6 XhoI T7

Xenopus tropicalis

David Kimelman BamH1? T3? HindIII? T7? in situ? Xenopus

A. Zorn ~800 bp Cla1 T7 Xenopus tropicalis


Somites, DA, PN, NT EcoRV-NotI SmaI T7 To generate probe for in situ Xenopus


Paul Krieg - Mercola Lab

BamH1-HindIII piece from Xnot (~2kb) into

KS+

Somites, eye, BA, PN, PD, heart, ventral

mesoderm

Vasculature - Arterial specific


Kidney - Pronephros Full length, NotI-SalI SalI or SmaI T7 To generate probe for in situ Xenopus

andrea SalI Xenopus tropicalis

Tomas Pieler 789bp BamHI T7 In situ Probe Xenopus laevis

NotI-SalI SalI or EcoRI or EcoRV T7 To generate probe for in situ Xenopus

IMAGE R1 T7 Xenopus tropicalis

NotI-SalI SmaI or EcoRI T7 To generate probe for in situ Xenopus

HindIII T3

HindIII NotI Xenopus tropicalis



Xenopus tropicalis


A. Zorn EcoRI T7 Xenopus tropicalis


XhoI T7 Xenopus tropicalis

Xenopus tropicalis

Aaron Zorn ~ 2.5-3 kb insert EcoR1 T7 Xenopus tropicalis

Aaron Zorn ~3kb EcoR1 T7 In situ Hybridization Xenopus tropicalis

EcoRI - XhoI SpeI T7

HindIII T3

Xenopus tropicalis


Liz Jones Sal1 SP6 To generate mRNA for injections

EcoRI T3 XbaI T7 To generate probe for in situ and mRNA for injection.

A. Zorn ~5.4 kb insert BamHI T7 Xenopus tropicalis


somite, muscle Full length, EcoRV-NotI SmaI T7 To generate probe for in situ Xenopus

SalI T3

1194bp XhoI-XbaI NotI SP6 To generate mRNA for injection Xenopus

2.5 kb - Full length RI T7 Xenopus tropicalis

Heart, hindbrain - Neural - neural tube,

eyes, PN


Heller and André W. Brändli


Xenopus tropicalis

Nicole Heller 3kb EcoRI-XhoI EcoRI T7 XhoI T3 in situ probe Xenopus

Mustafa EcoRI T7 Xenopus tropicalis

Invitrogen Xenopus tropicalis



Sal1 T7

Full length, SalI-NotI SalI or SmaI or EcoRI T7 To generate probe for in situ Xenopus

EcoRI Xenopus tropicalis

XhoI T3

blood vessels NotI-SalI SalI or SmaI T7 To generate probe for in situ Xenopus

Hugh Woodland EcoR1 T7 in situ probe

Hugh Woodland NotI SP6 RNA Injection

ClaI/XhoI NotI To generate mRNA for injections.


Kidney - Pronephros and duct, CNS

Proliferating cells, DA, HSC


Hoppler Lab HindIII T7

Peter Vize SalI/SmarI T7 In situ Probe Xenopus laevis?

Xenopus tropicalis

3089bp BamHI T7 3089bp in situ probe Xenopus

1104bp NotI SP6 RNA injection Xenopus

SalI T7

mustafa KpnI T7 To generate probe for in situ Xenopus tropicalis

lymphatic endothelium SalI-NotI SalI or SmaI T7 To generate probe for in situ Xenopus

Xenopus laevis


T takabatake 883bp EcoRI-HindII EcoRI SP6 To generate probe for in situ Xenopus

Katja Koebernick 4.2kb XhoI-XbaI BamHI T3 NotI SP6 To generate probe for in situ and mRNA for injections Xenopus

T takabatake 4.76kb EcoTI-XhoI ? NotI or XbaI T7 To generate probe for in situ Xenopus

SalI Xenopus tropicalis

NotI-SalI EcoRV or RsrII T7 To generate probe for in situ Xenopus

EcoRI-XhoI BamHI or ClaI or EcoRI T7 To generate probe for in situ Tropicalis

PCR T3/T7 T7


Blood - Myeloid NotI-SalI SalI T7 To generate probe for in situ Xenopus

Kenneth Kao 590bp EcoRI/XhoI NotI SP6 Capped RNA Xenopus laevis

Kenneth Kao StuI/XhoI NotI SP6 Capped RNA Xenopus laevis

Endoderm - Mesoderm - Endomesodermal cell

fate in Spemann's organizer

Notochord, eyes, lateral plate, somites

Head, neural tube, somites, tailbud, branchial arches

Eyes, brain, neural tube, somites,

branchial arches


Kenneth Kao EcoRI NotI SP6 Capped RNA Xenopus laevis

Kenneth Kao EcoRI/XhoI NotI SP6 Capped RNA Xenopus laevis

Blumberg lab NotI T7 XhoI T3 in situ probe and mRNA for injection Xenopus

Xenopus laevis

RNA injection Xenopus laevis





Xenopus laevis

Xenopus laevis


Aldo SmaI-NotI ClaI T7 To generate probe for in situ

Xenopus tropicalis

Head, brain, eyes, PCV SalI-NotI EcoRI T7 To generate probe for in situ Xenopus

FseI - Asc I T7 sp6

T7 sp6 To generate probe for in situ and mRNA for injection.

Eyes, head, BA, somites NotI-SalI SalI or SmaI T7 To generate probe for in situ Xenopus

T7

Hugh Woodland BamH1 SP6 RNA Injection

T7 SP6 Xenopus tropicalis

PCR T3/T7 T7

ClaI/XhoI BamHI T7 NotI SP6 To generate probe for in situ and mRNA for injection.


A. Zorn R1 T7 Xenopus tropicalis

W. Dan Tracey 2027 bp, EcoR1 Sal I T7 full length in situ hybridisation Xenopus


Neural - Neurons Full length EcoRI T7 To generate probe for in situ Xenopus

Somites, eyes, BA EcoRV-NotI ClaI T7 To generate probe for in situ Xenopus


Liz Jones 1.4 kb SacII T3 KpnI T7 in situ clone Xenopus laevis

Liz Jones 3 kb EcoRI T7 XhoI T3 in situ clone Xenopus laevis

Liz Jones 3 kb EcoRI T7 XhoI T3 Xenopus laevis

BamHI T7

Asp718 SP6


sFRP-2 2Kb EcoR1 SP6 Xho1 T7 xenopus

NotI SP6

2.3kb BamHI T3 To generate probe for in situ Xenopus

Aaron Zorn ~2 kb BamHI T7 To generate probe for in situ. Xenopus tropicalis

Aaron Zorn not sure of length BamH T7 To generate probe for in situ. Xenopus tropicalis

Xenopus tropicalis

Richard Harland Xenopus tropicalis

Aaron Zorn Cla1 T7 Xenopus tropicalis

SalI T7 Xenopus tropicalis

Somite, muscle SalI-NotI SalI or EcoRI or ClaI T7 To generate probe for in situ Tropicalis

Xenopus tropicalis

NcoI Sp6

Blood - Primitive blood, VBI, Otic vesicle, olfactive placode

Rohon beard cells, Embryonic

haemangioblast, VBI, myeloid cells, DA-HSC

in situ cloneTo generate mRNA for injection and Probe for in situ.





Aaron Zorn 1.5 kb Xenopus tropicalis


Liz Jones Sma1 T7 To generate probe for In situ

Xenopus tropicalis

Blood - VBI SalI-NotI Sal or SmaI T7 To generate probe for in situ Xenopus

Xenopus tropicalis

Peter Vize SmaI T7 In situ Probe Xenopus tropicalis

Liz Jones Sma1 T7 To generate probe for In situ

see paper Thomsen, J ~3kb EcoR1 T3 or T7 NotI SP6 Xenopus


Heart - Endocardium C. Hill lab EcoRI T7 T3 in situ and injections Xenopus


Hong Ren Jon Graff NotI SP6 To generate mRNA for injection


Xenopus tropicalis





Head, tailbud Katja Koebernick 1155bp ApaI SP6 To generate probe for in situ Xenopus

Richard Harland Xenopus tropicalis


Neural crest, EMT 0.7kb, EcoRI BglII SP6 To generate probe for in situ Xenopus

ClaI SP6

Aaron Zorn ~2kb BamH1 T7 Xenopus tropicalis

Smad10 ORF 1780bp clonned between Bam

and Eco sites




Neural crest, EMT 1kb, EcoRI BglII SP6 To generate probe for in situ Xenopus

Neural crest, EMT 0.8kb, EcoRI BglII SP6 To generate probe for in situ Xenopus

SalI-NotI SalI or SmaI T7 To generate probe for in situ Tropicalis

Aaron Zorn HindIII T7 Xenopus tropicalis

Aaron Zorn HindIII T7 Xenopus tropicalis

tim grammer To generate probe for in situ Xenopus tropicalis

Tim grammer AscI SP6 to generate RNA for injection Xenopus tropicalis


BamHI T7 AscI Xenopus tropicalis


1.6 kb Sma I T7 1.6Kb XhoI T3 1.6kb Insitu probe template Xenopus

1.4kb EcoRI T7 1.4kb XhoI T3 1.4kb In vitro transcription template for insitu probe Xenopus


Xenopus tropicalis

ApaI SP6

Blood - Blood vessels Full length, NotI-SalI SalI or SmaI T7 To generate probe for in situ Xenopus

A. Zorn 3 kb insert? Cla1 T7 Xenopus tropicalis

A. Zorn ~1.5 kb insert Cla1 T7 Xenopus tropicalis

EcoRI/XhoI ClaI

EcoRI T7



insert about 1.7 kb inserted in EcoR1/Not1

about 2.55 kb inserted into EcoRI/Not1

Endoderm - Whole Endoderm




SmaI T7

Blood - Blood vessels NotI-SalI SmaI T7 To generate probe for in situ Xenopus

Rebecca Spokony 1400 NcoI SP6 Soc1 T7 To generate probe for in situ and mRNA for injection. Xenopus Laevis

Robert Old 1859 bp full cDNA NotI T3 XhoI T7 in situ/injections Xenopus

aldo Full length, EcoRV-NotI SmaI T7 To generate probe for in situ Xenopus tropicalis



Maggie ~2kb EcoRI- XhoI Not1 Sp6 ~2kb RNA for injection Xenopus


Ryuichi Nishinakamura EcoRI T7 NotI T3 in situ / injections Xenopus

R Nishinakamura 3kb KpnI-BamHI BamHI T3 Xenopus

R Nishinakamura 3kb KpnI-BamHI BamHI T3 To generate mRNA to be used as control for Xstat3EnR Xenopus

Muriel Umbhauer XbaI T7 XhoI T3 in situ Xenopus

Elizabeth Pownall 700bp XbaI T3 In situ Probe Xenopus tropicalis

Full length, SalI-NotI SmaI or EcoRI T7 To generate probe for in situ Xenopus

BamHI T7 NotI SP6

NotI SP6

Maggie 2.216kb EcorI Ssp1 T7 305 216 RPA and Wish probe Xenopus

Aaron Zorn unknown insert length BamH1 T7 Xenopus tropicalis

EcoR1 T7 Xenopus tropicalis

Aaron Zorn ~3kb EcoR1 T7 In situ Hybridization Xenopus tropicalis

BglII T7

EcoR1 SP6 synthesis of RNA and inhibition of Xbra xenopus

H Shibuya 2kb SacI T7 ? T3 To generate probe for in situ Xenopus

Liver - Mesoderm - Blood - Liver

precursors, ventral mesoderm,

Haemangioblasts?

Blood -Haemangioblast,

Myeloid, HSC

3 kb EcoRI-NotI fragment

To generate XSTAT3 dominant negative mRNA for injection

DNA biding domain of Xbra (up to Cla1 site) fused to the engrailed repressor and a myc

tag




aldo 1340, EcoRI-ClaI Xho I SP6 in situ Xenopus

Todd Evans NheI T7 xenopus SCL for injection Xenopus

branchial arches Full length, EcoRV-NotI SacII T7 To generate probe for in situ Xenopus

EcoR1 SP6 HindIII T7 xenopus

SphI SP6 To generate Tbx20 probe Xenopus

Xba1 T7 xenopus

Not1 T7 Nco1 Sp6 xenopus

NotI SP6 RNA

Xenopus tropicalis

Liz Jones To generate mRNA for injections

Oliver Destree XbaI T7 Capped RNA

Oliver Destree XbaI T7 Capped RNA

SP6 Xba T7 To generate mRNA for injection and probe for in situ. Xenopus laevis

Liz Jones Not1 T3 To generate mRNA for injections

XbaI sp6 To generate mRNA for injection

Olaf van den Broek inserted into EcoRV site XbaI T7 Capped RNA




Kidney Liz Jones XBaI T7 To generate mRNA for Injection. Xenopus laevis

Xenopus tropicalis

Full length, EcoRI-XhoI BamHI or ClaI or EcoRI T7 To generate probe for in situ Tropicalis

Andrea EcoRI Xenopus tropicalis

Blood - VBI SalI-NotI SmaI T7 To generate probe for in situ Xenopus

Blood - Neural - VBI, DLP, Endocardium,

Blood Vessels, Brain, Neural Tube.

Inserted between BamHI and HindIIIEcoRI

digest gives two fragments - 1.9 & 0.5

kb

Sau3A1 - Pst1 fragment (1367-1984) of Xenopus Tbx2 cDNA

site BamH1-Pst1

Heart - Neural Crest - Heart, DA, brain

Brian & Pagratis, Conlon lab

4.14 kb accession number AB032942 site

EcoR1 Xho1insert 399bp

(accession number: AB032944)pcr

fragment of xenopus tbx5 cDNA

xTCF3 dominant negative




Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

heart-specific marker 927bp NotI / EcoRI NotI T7 In situ Probe ?

Liz Jones Sal1 T7 To generate probe for In situ

Sal1 Xenopus tropicalis

Peter Vize Xenopus laevis

Sanger not full length - TNeu104j16 Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

SmaI T3 T7 To generate probe for in situ and mRNA for injection.



Hopwood T7 Eco RI T3 in-situ Xenopus




Aaron Zorn unknown Xenopus tropicalis

Aaron Zorn ~1.3 kb EcoR1 T7 Xenopus tropicalis



560bp EcoRI T7 T3 To generate probe for in situ and mRNA for injection.

A. Zorn ~7 kb insert? Xenopus tropicalis

A. Zorn 5 kb insert??? Cla1 T7 Xenopus tropicalis


Kathryn Tonissen and Paul Krieg

Neural - Primary neurons

cloned into EcoRI site of Bluescribe - size not

knownto be confirmed -

HindIII




NotI SP6

BamHI SP6



BamHI T7 To generate probe for in situ.

Blood - VBI EcoRV-NotI SmaI or EcoRI T7 To generate probe for in situ Xenopus

Asc1 xenopus

NotI-SalI SalI-SmaI T7 To generate probe for in situ Xenopus


Blood - Haematopoietic NotI-SalI SmaI T7 To generate probe for in situ Xenopus

3.3kb BamH1 T7 2.9kb WISH probe Xenopus

122aa, BamHI-XbaI NotI SP6 740bp To generate mRNA for injection Xenopus



Xenopus tropicalis

Xenopus tropicalis

A. Zorn ~2kb insert Xenopus tropicalis

Hugh Woodland XhoI SP6 RNA Injection




Directionally clonned between EcoR1 5' and

XhoI at the 3' end

Xenopus Strabismus 2.15kb

Embryonic haemangioblast, myeloid cells, DA




Mary Lou King coding region T7 Not1 SP6 sense/antisense RNA synthesis xenopus

Eco R1 T3 1.2 Kb RNA for injection Xenopus


IMAGE EcoR1 T7 Xenopus tropicalis


entire Xom cDNA Bgl II BamH1 SP6 Microinjection of RNA xenopus

SP6 pst1 Microinjection of RNA xenopus

A. Zorn 0.75 kb ClaI T7 Xenopus tropicalis

Oliver Destree ? ?

EcoRI - 1.5kb ? ?

1.8 SalI/NotI SalI T7 AscI SP6 To generate probe for in situ and mRNA for injection.

BamH1 SP6 To generate mRNA for injection xenopus

BamH1 SP6 To generate mRNA for injection Xenopus

VBI EcoRI XbaI SP6 SacI or ClaI T7 To generate probe for in situ and mRNA for injection Xenopus

Not tested 912 bp SacI T7 To generate probe for in situ Xenopus

Not tested 912 bp SacI T7 To generate probe for in situ (sense probe) Xenopus

Kidney Liz Jones BamHI SP6 To generate mRNA for Injection. Xenopus laevis

Kidney Liz Jones SacI T7 SacI T7 To generate probe for In situ Xenopus tropicalis

Kidney Liz Jones PstI T7 NcoI SP6 To generate probe for In situ Xenopus tropicalis

EcoR1 T7 NotI SP6

good question EcoRI SP6 Wnt3A expression construct xenopus

977 bp Xho 1 / Hind 2 (5'-3')

Bgl II fragment of Xom (+76 to +582) cloned

into SP64T

Oliver Destree - Moon Lab

Xho1-Bgl11 fragment of Xwnt11 amplified by PCR from pGEM-X9 aka

10.1.4 (Xwnt11 supplied by Doug

Melton)

Dra1 - BamH1 fragment of pGem-X9 aka 10.1.4 (from Doug

Melton) was cloned into pSP64TXB


Kidney Liz Jones SalI SP6 To generate mRNA for Injection. Xenopus laevis

Kidney Liz Jones XbaI SP6 To generate mRNA for Injection. Xenopus laevis

Kidney Liz Jones EcoRl SP7 To generate mRNA for Injection. Xenopus laevis

Kidney Liz Jones EcoRI T7 NotI SP6 To generate probe for In situ

Kidney Liz Jones HindIII T3 To generate mRNA for Injection. Xenopus laevis

SalI T7 AscI Sp6 To generate probe for in situ and mRNA for injection.

Hoppler et al. 794 bp - - - Not I SP6 1100 Template for RNA for injection Xenopus

Aaron Zorn 1.6 kb ClaI T7 AscI Sp6 Xenopus tropicalis

Kidney Liz Jones BamH1 SP6 To generate mRNA for Injection. Xenopus laevis

sp6

Kidney Liz Jones ClaI T7 PstI T3 To generate probe for In situ Xenopus laevis

Kidney Liz Jones ClaI T7 PstI T3 To generate probe for In situ Xenopus tropicalis

Kidney Liz Jones SacII SP6 To generate probe for In situ xenopus tropicalis

Kidney Liz Jones HindIII T7 BamHI T3 To generate probe for In situ Xenopus laevis

Kidney Liz Jones SacII SP6 To generate probe for In situ Xenopus tropicalis

Liz Jones Hind III + Sst I SP6 To generate mRNA for injections

Liz Jones Sac1 T7 To generate probe for In situ

Sive, Hazel 800bp NotI-EcoRI NotI T7 EcoRI,HindIII, XhoI T3 To generate probe for in situ and mRNA for injection. Xenopus Laevisst12-48 cement gland head ectoderm


Aaron Zorn 2.2 kb Xenopus tropicalis


EcoRI Sp6

BglII T7

Oliver Destree ? ?

? ?

NK specific Full length, EcoRV-NotI SmaI or EcoRI T7 To generate probe for in situ Xenopus

Liz Jones 1 kb SacII T3 KpnI T7 in situ clone Xenopus laevis

ClaI T7

Aaron Zorn ~2 kb BamH1 T7 In situ Hybridization Xenopus tropicalis

EcoRI T7 In Situ Probe Xenopus laevis

Haematopoietic Full length, NotI-SalI SalI or SmaI T7 To generate probe for in situ Xenopus

James McGann T7 Xenopus tropicalisJames McGann T7 Xenopus tropicalis

Xenopus tropicalisXenopus tropicalisXenopus tropicalisXenopus tropicalisXenopus tropicalis


EXRC Number Gene symbol Gene Name Gene synonyms

LJ-3 tcf21 gut/kidney TF

LJ-8 tcf21 TF

LJ-12 glb1 n/a

LJ-24 tcf12 HEB

LJ-25 tcf4 TF



capsulin, pod 1, pod1



galactosidase, beta 1


Human Entrez Gene]

beta-galactosidase

transcription factor 12 (HTF4, helix-loop-helix

transcription factors 4)

muscle and immune TF


tcf-4, Xtcf-4, Xtcf4


A1


Probe name Plasmid name Pictures Atribution

not needed p

not in xenbase no pictures n/a

Bgal Bgal pCS2 plus not needed p

HEB pBS HEB pBS no pictures p

not in xenbase TF4 pBS TF4 pBS

Xenbase Number

XB-CLONE-11536577

mPOD 1 pCS2+ (rescue)

mPOD 1 pCS2+ rescue

mHEB pCS2+ I.M.A.G.E

BC037097

mHEB pCS2T image

BC037097

XB-CLONE-11536609

XB-CLONE-11919419


Sequence Paper Vector Antibiotics Special Features

Mus musculus pCS2+ Amp

Amp

pCS2+ Amp

pBLUESCRIPT Amp

pBLUESCRIPT Amp

from IMAGE clone

NP_035675

Mus Musculus Tcf12

mHEB pCS2T image

BC037097

This may be in pBS KS and is

the mouse clone

Mus musculus targeted non-conditional, lacZ-tagged

mutant allele

Mus musculus transcription

factor 12

Mus musculus transcription

factor 4 (Tcf4)

Original Author

Liz Jones

Liz Jones

Liz Jones

Liz Jones EcoRV T7

Liz Jones

Expression pattern

Insert size and sites

Antisense Linearisation

Antisense Polymerase

Antisense size Sense size

Hind III + Sst I SP6

Xho1 Sp6

Sense Linearisation

Sense Polymerase

Purpose Organism

To generate mRNA for injections


Mus musculus (house mouse)


To generate probe for In situ

To generate probe for In situ

Gene symbol Gene Name

91-KL smad6 TGFb TF

LJ-16 tcf3

15-CC smad1 TGFb TF

16-CC smad2 TGFb TF

EXRC Number

Gene synonyms


Xenbase Number


madh6, Xsmad6

XB-CLONE-12442487


immunoglobulin enhancer

binding factors E12/E47


tcfe2a

transcription cofactor

XB-CLONE-11536625


Xsmad1, smad-1, Xmad1

XB-CLONE-12442195


XSmad2, smad-2

XB-CLONE-12442198


A1


Probe name Plasmid name Pictures Atribution Sequence

Smad6 Smad6-cs105 not needed p homo sapiens

hE47 pGEMT hE47 pGEMT not needed p

Smad1 Smad1 not needed p

Smad2 Smad2 not needed p

Homo sapiens transcription factor 3

(TCF3)

PREDICTED: Homo sapiens SMAD family member 1 (SMAD1)

PREDICTED: Homo sapiens SMAD family member 2 (SMAD2)

Add as synonim?-Maya Piccinni

G3

Add as synonim? -Maya Piccinni

Paper Vector Antibiotics

pCS105 Amp

pGEMT Amp human E47

pCS105 Amp

pCS105 Amp

Special Features

Expression pattern

Full length Smad 6 from HuT2 1.5kb

Signal Transducers

Signal Transducers


Original Author Antisense size

Liz Jones




Sense size Purpose

SP6

Sfi1 T3

AscI SP6

AscI SP6

Sense Linearisation

Sense Polymerase

To generate mRNA for injection




Organism

Xenopus???/Human??

Xenopus???/Human??

EXRC Number Gene symbol Gene Name

P1-1

P1-2

P1-3

P1-4

P1-5

P1-6

P1-7

P1-8

P1-9

Gene synonyms

Gene Function (for

GMAG)Xenbase Number

basic promoter

not in xenbase

elastase promoter

not in xenbase

crystallin promoter

not in xenbase

crystallin promoter

not in xenbase

upstream activating sequence

not in xenbase

upstream activating sequence

not in xenbase

cuticle collagen promoter

not in xenbase

Fly selection marker

not in xenbase

puromycin responsive promoter

not in xenbase


A1


p2-1

p2-2

p2-3

p2-4

p2-5

p2-6

p2-7

p2-8

p2-9

p2-10

cardiac actin promoter

not in xenbase

ubiquitous promoter

not in xenbase

ubiquitous promoter

not in xenbase

ubiquitous promoter

not in xenbase

muscle promoter

not in xenbase

neural promoter

not in xenbase

neural promoter

not in xenbase

upstream activator sequence

not in xenbase

neural promoter

not in xenbase

uncharacterised promoter

not in xenbase

p2-11

p2-12

p2-13

p2-14

p2-15

p2-16

p2-17

p2-18

p2-19

p2-20

vascular promoter

not in xenbase

uncharacterised promoter

not in xenbase

neural promoter

not in xenbase

myeloind promoter

not in xenbase

ubiquitous promoter

not in xenbase

ionophore promoter

not in xenbase

heat shock inducibe promoter

not in xenbase

blood promoter

not in xenbase

neural promoter

not in xenbase

notocord promoter

not in xenbase

p2-21

p2-22

p2-23

p3-1

p3-2

p3-3

p3-4

p3-5

p3-6

p3-7

ubiquitous promoter

not in xenbase

blood promoter

not in xenbase

ionophore promoter

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

p3-8

p3-9

p3-10

p3-11 epitope tags

p3-12

p3-13

p3-14

p3-15

p3-16

p3-17

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

p3-18

p3-19

p3-20

p3-21

p3-22

p3-23

p3-24

p3-25

p3-26

p3-27 TF

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

tyr recombinase

not in xenbase

not in xenbase

p3-28

p3-29

p3-30

p3-31

p3-32

PD-1

PD-2

PD-3

PD-4

toxic viral protein

not in xenbase

tyr recombinase

not in xenbase

receptor-fluorescent

proein fusionnot in

xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

trnsgenesis facilitators

not in xenbase


not in xenbase


not in xenbase

transgenesis facilitators

not in xenbase

JW-2252 not known

JW2380

JW-2240

JW-2142

JW-1468

149-SPH

133-SPH

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

protease inhibitor

not in xenbase

protease inhibitor

not in xenbase

35-SPH eef1a1

45-SPH vector

37-SPH GFP

39-SPH

48-SPH undefined

56-SPH undefined nuclear b-gal

145-SPH

EF-1 alpha, eef1a-s,

elongation factor-1 alpha

translation elongation

not in xenbase

heat shock promoter

not in xenbase

Fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

not in xenbase

ribosomal processing

not in xenbase

106-SPH pcbd2

107-SPH

27 -NP

51-KL

LJ-13

LJ-56

LJ-57

LJ-58

LJ-59

transcription modulator

not in xenbase

protease inhibitor

not in xenbase

fluorescent protein

not in xenbase

fluorescent protein

not in xenbase

Fluorescent Protein

not in xenbase

signalling molecule

not in xenbase

signalling molecule

not in xenbase

signalling molecule

not in xenbase

signalling molecule

not in xenbase

LJ-60

LJ-61

LJ-62

LJ-64

LJ-52 vector only

LJ-78

LJ-54

fluorescent protein

not in xenbase

signalling molecule

not in xenbase

kidney/urogenital TF

not in xenbase

fluorescent protein

not in xenbase

not in xenbase

phosphodiesterase

not in xenbase

signalling molecule

not in xenbase

LJ-55

LJ-53

1852 Vector

1856 Vector

signalling molecule

not in xenbase

fluorescent protein

not in xenbase

Not in xenbase

Not in xenbase


not needed ok

not needed ok

not needed ok

not needed ok

not needed ok

not needed ok

p1 rol-6 p1 rol-6 not needed ok

p1 mini white p1 mini white not needed ok

not needed ok

p1 aCE2x6 eCFP

p1 aCE2x6 eCFP

p1 elastase GFP

p1 elastase GFP

p1 gamma crysmCherry

p1 gamma crysmCherry

p1 gamma crys venus

p1 gamma crys venus

p1 5xUAS mCherry

p1 5xUAS mCherry

p1 14xUAS lynGFP

p1 14xUAS lynGFP

p1-PGK:: PuroRes

p1-PGK:: PuroRes

not needed ok

p2 CMV p2 CMV not needed ok

p2 Ef1a p2 Ef1a not needed ok

p2 ubiquitinC p2 ubiquitinC not needed ok

p2 CarA p2 CarA not needed ok

p2 NBT p2 NBT not needed ok

p2 Pax6 p2 Pax6 not needed ok

p2 14xUAS p2 14xUAS not needed ok

not needed ok

p2-nectin-2 p2-nectin-2 not needed ok

p2 CAG p2 CAG

p2-HB9 p2-HB9

p2-flk1 p2-flk1 not needed ok

p2-vast p2-vast not needed ok

p2-sox3 p2-sox3 not needed ok

p2-Xlurp p2-Xlurp not needed ok

p2-vimentin p2-vimentin not needed ok

p2-foxi1 short p2-foxi1 short not needed ok

p2-hsp70 p2-hsp70 not needed ok

p2-lmo2 short p2-lmo2 short not needed ok

p2-pax3 p2-pax3 not needed ok

p2-bra p2-bra not needed ok

p2-ROSA 26 p2-ROSA 26 not needed ok

p2-lmo2 long p2-lmo2 long not needed ok

p2-foxi1 long p2-foxi1 long not needed ok

p3-VenusGFP p3-VenusGFP not needed ok

p3-eYFP p3-eYFP not needed ok

p3-Gal4 p3-Gal4 not needed ok

p3-HyPerYFP p3-HyPerYFP not needed ok

p3-mCherry p3-mCherry not needed ok

not needed ok

p3-mKaede p3-mKaede not needed ok

p3-Midori-Ishi Cyan

p3-Midori-Ishi Cyan

not needed ok

p3-mKate p3-mKate not needed ok

p3-mTFP p3-mTFP not needed ok

not needed ok

not needed ok

not needed ok

P3-mCerulean P3-mCerulean not needed ok

p3-eCFP p3-eCFP not needed ok

p3-dTomato p3-dTomato not needed ok

p3-rTA-M2 p3-rTA-M2 not needed ok

p3-Kusabi Orange

p3-Kusabi Orange

p3-Myc HA tag

p3-Myc HA tag

p3-KatRFP Flk-1 int

p3-KatRFP Flk-1 int

p3-Katushka RFP

p3-Katushka RFP

Maybe ccdb???

J51

Maybe ccdb???

p3-deGFP p3-deGFP not needed ok

p3-H2BGFP p3-H2BGFP not needed ok

p3-Azami p3-Azami not needed ok

p3-Kikume p3-Kikume not needed ok

p3-Flpe p3-Flpe not needed ok

not needed ok

not needed ok

p3-Cre-ERt2 p3-Cre-ERt2 not needed ok

p3-Cre-NLS p3-Cre-NLS not needed ok

p3-Gal4:VP16 p3-Gal4:VP16 not needed ok

p3-H2B mCherry

p3-H2B mCherry

p3-Ds Red2-nuc

p3-Ds Red2-nuc

Maybe ccdb???

p3-M2-Toxin p3-M2-Toxin not needed ok

not needed ok

not needed ok

not needed ok

not needed ok

not needed ok

not needed ok

not needed ok

not needed ok

p3-mER-Cre-mER

p3-mER-Cre-mER

p3-XFD-Venus (eGFP)

p3-XFD-Venus (eGFP)

p3-loxPeCFP loxPmCherry

p3-loxPeCFP loxPmCherry

p3-memGFP-2A-mCherry

H2B

p3-memGFP-2A-mCherry

H2B

pDEST Transgenesis

4-1 (Tol2/ ISce-1-Tol2)

pDEST Transgenesis

1-1 (Tol2/ ISce-1-Tol2)

pDEST Transgenesis

4-2 (Tol2/ ISce-1-CH4-SAR/Tol2)

pDEST Transgenesis

1-2 (Tol2/ ISce-1-CH4-SAR/Tol2)

pDEST Transgenesis

4-3 (Tol2/ ISce-1- CH4-SAR/Tol2/P-element)

pDEST Transgenesis

1-3 (Tol2/ ISce-1- CH4-SAR/Tol2/P-element)

pDEST Transgenesis

4-4 (Tol2/ ISce-1- CH4-

SAR/ISce-1/Tol2/P-element)

pDEST Transgenesis

1-4 (Tol2/ ISce-1- CH4-

SAR/ISce-1/Tol2/P-element)

Maybe ccdb???

CS10R ok

rfp seq

H2B-mRFP ok

ok

ok

pHSHSG3 pHSHSG3 p

pHSHSG2 pHSHSG2 p

CS2-RFP wGBD

Histone-2B - fused in frame to

mRFP

CS107-GFP-Moe

GFP-moe fusion

protein, c-msn-GFP

CS2+-memEGFP

EGFP fused to ras

farnesylation sequnce.label

s cell membranes

Maybe ccdb???

pXeX pXeX not needed p vector

pHS1 pHS1 p

nGFP pCMVnGFP p

pNβTnGFP pNβTnGFP p

pHSGFP3 pHSGFP3 p

pSP6nβgal pSP6nβgal

pENP1 pENP1


bug is resolved


bug is resolved


bug is resolved


bug is resolved


bug is resolved

cant find sequence file

not good with t3

pHS2 pHS2 ? ?

pHSHSG pHSHSG not needed p

GFPpSPJC2L GFPpSPJC2L not needed p p

pHSdnwnt11 pHSdnwnt11

pHSlim1 pHSlim1

cloning vector sequence

with T7 and T3 no

sequece aligment with

SP6

cs 2- nuc GFP2

cs 2- nuc GFP2

vector sequences

Cs2-membrane

RFP

CS2-membrane

RFPvector

Sequence

pHSwnt11-HSGFP

pHSwnt11-HSGFP

pHSdnwnt11-HSGFP

pHSdnwnt11-HSGFP

pCSGFP3 pCSGFP3

pCSwnt4 pCSwnt4

pCSlim1 pCSlim1

pElastaseGFP pElastaseGFP

pHS1 pHS1

enpp4 D36N enpp4 D36N

pHSwnt4 pHSwnt4

For REMI - all transgenic constructs from liz.-Maya Piccinni

G102

For REMI - all transgenic constructs from liz. -Maya Piccinni

pHSwnt11 pHSwnt11

pHSGFP3 pHSGFP3

p

p

transposase with polyA tail

pCS2-FA-transposase

pMTB2-ESS-Del

pMTB2-ESS-Del


Paper Vector Antibiotics

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.

spectinomycin

pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.

spectinomycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin


spectinomycin

pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.

pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.

Maybe ccdb???

kanamycin

kanamycin

kanamycin

kanamycin

kanamycin

pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.pTransgenesis, A cross-species, modular transgenesis resource. Love et .al. 2011.


ampicillin / chlorampheni

col



col



col



col

Maybe ccdb???

CS10R Amp

pCS2+ Amp

p13-pCS Amp

BMC Cell Biology 1:1(2000) CS107 Amp

pCS2+

Wheeler et al 200 CB 10:849 ?

J Cell Biol. 2005 Jan 31;168(3):429-39.

Maybe ccdb???

pXeX amp

pHS1 amp

pCS2+ AMP

pHS1 amp

pGEM3Z blue amp

?

pXeX, a vector for efficient expression of clonned sequences

in Xenopus Embryos. PMID: 7926804 Gene 1994 sep 30; 147

(2) : 223-6

G. N. Wheeler et al. CB 10: 849 (2000)

Kroll & Amaya Dev 122,3173 (1996)

G. N. Wheeler et al. CB 10: 849 (2000)

Injected Xwnt-8 RNA acts early in Xenopus embryos to promote

formation of a vegetal dorsalizing center. Smith and Harland, Cell.

1991 Nov 15;67(4):753-65.

Direct regulation of the Xenopus engrailed-2 promoter by the Wnt

signaling pathway, and a molecular screen for Wnt-

responsive genes,con®rm a role for Wnt signaling

during neural patterning in Xenopus - Mechanisms of

Development 87 (1999) 21±32

?

?

PCS2 Amp

pCS2+ Amp

pSPJC2L Amp

Tételin and Jones (unpublished) pHS1 Amp




Modified by Haseloff and Pines. pCS Amp

Tételin and Jones (unpublished) pCS Amp

Tételin and Jones (unpublished) pCS Amp

Caroline Beck, 1999. pIT2 Amp

Wheeler et al., 2000 pHS1 Amp

pCS2+




Wheeler et al., 2000 pHS1 Amp

pCS2 Amp

pMTB2 Amp


Special Features Expression pattern

Maybe ccdb???

GTPase activity

Nuclear/chromosome label

Membrane label

Vector for cloning of GFP-fusions. New vector~! This vector was designed to allow easy shuttling of GFP

fusion clones from Clontech eGFP vectors into a CS-family Xenopus expression vector. The origin of this vector is CS107.The MCS

between BamHI and EcoRI is modified.

------------------------------------------------------------------------SP6 promoterSmaI, SacI and XbaI, SpeI, BamHI, EcoRV, PstI, EcoRI, SalI,

NotI, StuI, XhoI T7 promoterCS10R-Sp6 (multi-cloning site : 28(smaI)-

112(XhoI)GGAACTGCTCTTTTGAGGATCTTCCCCCGGGGGAGCTCTAGAGCACTAGTCGGGATCCCGGATATCTGCAGAATTCGTCGACAGGCCAATCTGGCCGCGGCCGCAAGGCCTCTCGAGCCTCTCGCCCTATAGTGAGTCGTATT

A

Actin label. It is from #2141 (from Furthmayr)it is subcloned with BamHI(partial digestion) and XhoI.

Maybe ccdb???

pCS2+ with nGFP insert between ClaI and XhoI.

insert: Hind III fragment containing nuc-beta-gal

followed papers but did not find info

This plasmid contains the transcription regulatory reagions from the Xenopus laevis elongation factor-1α-encoding gene (EF-1α).

Expression of cloned sequences commencess in blastula-stage embryos, coincident with transcriptional activation of the embryonic

genome, and transcripts may persist until the tadpole stage of development.

A 330bp long HindIII-XbaI fragment from phspCAT (plasmid no. 21, obtained from Alan Wolffʼs lab) containing the hsp

promoter with TATA box was cloned into the pBluescript SK- (Anne Donaldsonʼs prep, plasmid no. 41) to obtain

plasmid pbshsp1 (SH30/6/98). pbshsp1 was then cut w/ XbaI, blunted with Klenow, cut with ScaI and cloned into a

pCS2+ (plasmid no. 1) which had been cut with HindIII, blunted with Klenow and ScaI cut to yield plasmid HS1

(SH9/7/98). Note that blunt ligation recreated functional XbaI site.

A BamHI - NotI fragment containing GFP3 coding and SV40 coding. SV40 Poly A was clonned into the BamHI and Not I sites of HS1.

This GFP3 construct enherited the SP6 “promoter”as its message leader. In the original pCXGFP3 construct the leader

was composed of beta-globin sequences.

membrane targeted fast-folding monomeric RFP

pHS1, Wheeler et al., 2000. dnwnt11 (1Kb), Tada and Smith, 2000

pHS1, Wheeler et al., 2000. lim1 (1Kb), Kodjabachian et al., 2001.

pHS2 (no 106) cut with NotI, Klenow, cut with Asp108. pHSGFP (no 45) cut with HindIII, Klenow, cut with Asp108, approximately 1500bp

band.

membrane labeling

pHSGFP3, Wheeler et al., 2000 and pHSwnt11, Tételin and Jones (unpublished). 2 heat shock promoters driving wnt11 and GFP.

pHSGFP3, Wheeler et al., 2000 and pHSdnwnt11, Tételin and Jones (unpublished). 2 heat shock promoters driving dnwnt11 and GFP.

GFP driven under the control of the CMV promoter.

GFP driven under the control of the elastase promoter.

Heat shock promoter with TATA box.

pHS1, Wheeler et al., 2000. wnt4 (1.2Kb) McGrew et al., 1992.

wnt4, (1.2Kb) McGrew et al., 1992, driven under the control of the CMV promoter.

lim1 (1Kb) Kodjabachian et al., 2001, driven under the control of the CMV promoter.

pHS1, Wheeler et al., 2000. wnt11 (1Kb), Ku and Melton, 1993.

GFP driven under the control of the heat shock promoter.

to make transposase mRNA with polyA tail; better translation than original construct

Modified from "pMTB2 cyto BirA 2A Cherry Ras" Contains new multiple cloaning site EcoR1-Sal1-Sph1 and other Sph1 site mutated

Antisense sizeOriginal Author




Chanjae Lee

Chanjae Lee

Hoppler Lab

Hoppler Lab

William Bement

Histone-2B - fused in frame to

mRFP

GFP-moe fusion

protein, c-msn-GFP

EGFP fused to ras

farnesylation sequnce.label

s cell membranes

1.6 kb Asp718-XhoI

ClaI / XhoI

Kristen Kroll

BamHI - NotI

Moon lab EcoRI - 6kb

Randy Moon

from Paul Krieg's Lab

Stephen Hopler

Stephen Hopler

Not1 Sp6

Liz Jones

Liz Jones Notl or Sall

Liz Jones Sall

Liz Jones XMnl

Liz Jones Sall

Stephen Hoppler

Stephen Hoppler

From Ying Gong/Fraser

Lab

Liz Jones Sall

Liz Jones Sall

Liz Jones Sall

Liz Jones XMnl

Liz Jones

Liz Jones Notl

Liz Jones Sall

Liz Jones Notl or Sall

Arif

Sense size Purpose Keywords

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Sense Linearisation

Sense Polymerase

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Gateway Clone

Destination clone

Destination clone

Destination clone

Destination clone

Vector

NotI/Sp6 Cdc42 sensor, RFP

NotI/SP6

AscI/Sp6

SP6 notI

H2B-RFP, RFP, Histone, H2B, Histone RFP

It is from #2141 (from

Furthmayr)it is subcloned with BamHI(partial digestion) and

XhoI.

GFP, GFP-moe, moesin, C-msn-GFP

for sense, cut w/ Not1

GFP, membrane GFP, memegfp,

memgfp

Transgenics (Linearize with NotI or Asp718)

Transgenics (Linearize with NotI or Asp718)

Vector

Vector

?

Asp718 SP6

XhoI SP6 Capped RNA

Transgenics

transgenic construct /

cloning vector

Transgenic & Capped RNA

NotI SP6

Sal1 SP6

Transgenics (Linearize with NotI, Asp108 or

SalI)


TRANSGENIC CONSTRUCTS








TRANSGENIC CONSTRUCTS.



NotI SP6



to make transposase

mRNA

Part of cloaning strategy to create

transgenic construct with cre

mediated activation

Organism

?

?

?

?

?

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis


4 KERATIN xk70

5 XHOXA7

7 aT4 Globin

8 XGATA2RPA

12 XGATA1A(S)

13 wt3GATAluc

Gene synonyms

Gene Function (for

GMAG)Xenbase Number

Information / Stock to

come

Ankyrin repeat protein

Not in xenbase


come

Hox-A7, XlHbox-3, Xhox-36, hox36,

xlhbox 3, antp,

XlHbox5

homeodomai

n transcription

factor

Not in xenbase

ALPHAT4GLOBIN


come

Globins and related

hemoproteins Not in

xenbase


come


zinc finger transcription

factor Not in

xenbase


come

xGATA-1, xgata1, gata-

1


factorNot in

xenbase


come


factorNot in

xenbase


Maya Piccinni:is on xenbase but has no sequence. Sequence held is not good has to be re-sequenced

Maya Piccinni:not in xenbase, no dna no info

A1


B6

Maya Piccinni: is on xenbase but has no sequence. Sequence held is not good has to be re-sequenced

B7

Maya Piccinni: not in xenbase, no dna no info

14 mut3GATAluc

15

17

18 pButmafK n/a

19 pSP64TxG5(s)

23 XGATA3RPA

23 XGATA3RPA

24 XGATA2enMT


come


factorNot in

xenbase

Xenopus GATA2 fingers


come


factorNot in

xenbase

XGATA6 senseRNA


come


factorNot in

xenbase


come


factor

Not in xenbase


come

xGATA-5, xGATA5,

xGATA-5a, xGATA5a, xGATA-5b, xGATA5b,

GATA-5, gata-4a


factor Not in

xenbase


come


factor Not in

xenbaseInformation /

Stock to come


factor Not in

xenbaseInformation /

Stock to come


factor Not in

xenbase

Maya Piccinni:no good sequence, sequence not in xenbase

Not in xenbase, no info about it, but have prep on stock

B11

Maya Piccinni: no good sequence, sequence not in xenbase

24 XGATA2enMT

25 pCSKAeFGF

25 pCSKAeFGF

28 XFD

29 d50

30 hoxA7

31 hoxB9


come


factor Not in

xenbase


come

efgf, HBGF-4, XEFGF, fgfe, fgf-4, Xfgf4,

Xfgf-4, fgf4-b

heparin-binding

growth factor Not in

xenbase


come


Xfgf-4, fgf4-b

heparin-binding


xenbase


come


Not in xenbase


come


Not in xenbase


come

Hox-A7, XlHbox-3, Xhox-36, hox36,

xlhbox 3, antp,

XlHbox5

homeodomai

n transcription

factor

Not in xenbase


comexhoxb9, hox

2.5

homeodomain


Not in xenbase


32 pGem7 Vector Vector

34 MG350

35 MG750

36 1.3X GATA2B

38

39 XPax2

40 EF1a


comeNot in

xenbase


come



factor Not in

xenbase


come



factor Not in

xenbase


come



factor Not in

xenbase

DNBMP4receptor (tBR)


come

XBMP-4, bmp-4, xbmp4,

bone morphogeneti

c protein-4, zyme,

bmp2b, ofc11,

bmp2b1

TGF-beta related peptide

growth factor

Not in xenbase


comeXPax2, XPax-

2, pax-2 paired box


Not in xenbase


come

ef1a, stnl, eef1al, ef-1-

alpha-2

Translation elongation factor EF-1 alpha/Tu

Not in xenbase

Maya Piccinni:Missing Vector info

Maya Piccinni:have to ask aldo for it again with indo

Maya Piccinni:have pictures but don’t have probe data


B26

Maya Piccinni: Missing Vector info

B27

Maya Piccinni: have to ask aldo for it again with indo

B29

Maya Piccinni: have pictures but don’t have probe data

41

42 zf eFGF

43 GFP

44 actinGFP

45 GATA3cDNA

46 pBSXhoX352

47 CAT WISH

Xgata6a cDNA


come



factor Not in

xenbase


come


Xfgf-4, fgf4-b

heparin-binding


xenbase


comeFluorescent

ProteinNot in

xenbase


comeFluorescent

ProteinNot in

xenbase


come



factor Not in

xenbase


comeNot in

xenbase


comeNot in

xenbase





48 G3fMT

49 G3fVP16

50 G3FLVP16

51 Xvent-1

52 Xom RPA

53

54 GATA3fen


come



factor Not in

xenbase


come



factor Not in

xenbase


come



factor Not in

xenbase


come

vent-1, Xvent-1, vent1

homeodomai

n transcription

factor

Not in xenbase


come

vent-2, vent2, xvent2,

xvent-2, Xom

homeodomain


Not in xenbase

psp64TGATA2'


come



factor Not in

xenbase


come



factor Not in

xenbase




B39


55 noggin

56 Xwnt-8

57

58 Xwnt-8

59 pCSKA noggin

60 pintallavis

61 noggin


come

nog-A, noggin 1, noggin,

nog1, noggin-1

extracellular BMP

antagonist Not in

xenbase


come

Xwnt8, Xwnt-8, wnt-8,

wnt8

secreted growth

factor/developmental

regulator

Not in xenbase

pCSKA Xwnt-8


come


wnt8

secreted growth


regulator

Not in xenbase


come


wnt8

secreted growth


regulator

Not in xenbase


come


nog1, noggin-1

extracellular BMP

antagonist Not in

xenbase


come

pintallavis, xfkh1,

FoxA4a, XFD-1, xkfh-1,

fkh1

forkhead domain


Not in xenbase


come


nog1, noggin-1

extracellular BMP

antagonist Not in

xenbase



62 goosecoid

63 chordin

68 G2en

69 Enr-pSP64T

70 G2b'myc

71 G2f

72 pBLCAT2 XCAT2


comeXgsc,

goosecoid

homeodomain


Not in xenbase


come chd, chordin,

X-chordin

extracellular BMP

antagonist, dorsalizing

factor

Not in xenbase


come



factor Not in

xenbase


cometranscription

factor Not in

xenbase


come



factor Not in

xenbase


come



factor Not in

xenbase


come


Not in xenbase

73 pSVCAT xcat1

74 paG2SVCAT

75 distalless

77 Xslu

78 Xsna

79 Xpax-3

80 Xdelta delta, lmpy


come

Ca2+-permeable

cation channel OSM-9 and related

channels (OTRPC family)

Not in xenbase


comeNot in

xenbase


comexdlx3, ai4, tdo, Xdll-2

homeodomain and LIM

zinc binding domain


Not in xenbase


come

xslug, xSlu, slug, Snail2,

XSnail2

zinc finger transcriptiona

l repressor Not in

xenbase


come

xSna, snail, sna, Xsnail,

snail1


l repressor Not in

xenbase


come

pax-3, Waardenburg syndrome 1,

xpax3

paired box transcription factor with

homeodomain

Not in xenbase


comeNot in

xenbase

83 XOTX2

84 XBMP4/200

85 XBMP4/900

86 pBSK chordin

88 RX217

93 pMX470

94 pMX613


come Xotx2, otx-2,

Xotx-2

homeodomai

n transcription

factor

Not in xenbase


come


bone morphogeneti

c protein-4, zyme,

bmp2b, ofc11,

bmp2b1


growth factor

Not in xenbase


come


bone morphogeneti

c protein-4, zyme,

bmp2b, ofc11,

bmp2b1


growth factor

Not in xenbase


comechd, chordin,

X-chordin

extracellular BMP


factor

Not in xenbase


come

abad, camr, erab, hcd2,

mhbd, hadh2, mrpp2, mrx17, mrx31, schad,

mrxs10, sdr5c1, hsd10

Short-chain alcohol

dehydrogenase/3-

hydroxyacyl-CoA

dehydrogenase

Not in xenbase


come

rpx, Xanf1, Xanf-1, Xanf,

XANF-2, Xanf2, anf2

homeodomai

n transcription

factor

Not in xenbase


comeNot in

xenbase




97 pGBT9

98 pAS1-CYH2

99 pYTH6

102 pbs KS+ vector

103 pBS SK+ vector

104 pSP35T-Chd

105 pMix1


come

Xgalectin-VIa, gal4, l36lbp, galectin-4,

gal6a sugar binding Not in

xenbase


come

Xgalectin-VIa, gal4, l36lbp, galectin-4,

gal6a sugar binding Not in

xenbase


comeNot in

xenbase


comeNot in

xenbase


comeNot in

xenbase


comechd, chordin,

X-chordin

extracellular BMP


factor

Not in xenbase


come

mixl1, mix.1, mix.2, mix2,

tMix

homeodomai

n transcription

factor

Not in xenbase




F72


F73


F74


109 luciferase

110

111

112

113

114

115


comeNot in

xenbase

Gata2Promoter


come



factor Not in

xenbase

Gata 2promoter


come



factor Not in

xenbase

gata2promoter


come



factor Not in

xenbase

Gata2promoter


come



factor Not in

xenbase

Gata2promoter


come



factor Not in

xenbase

Gata2promoter


come



factor Not in

xenbase

116

117

118

119 pCAT Basic xcat1

120

121

122

Gata2promoter


come



factor Not in

xenbase

Gata2promoter


come



factor Not in

xenbase

Gata2promoter


come



factor Not in

xenbase


come

Ca2+-permeable

cation channel OSM-9 and related

channels (OTRPC family)

Not in xenbase

Gata1promoter


come


1


factor Not in

xenbase

Gata1promoter


come


1


factor Not in

xenbase

Gata1promoter


come


1


factor Not in

xenbase

123

124

125 GSTfli1ETS

126 GSTfli1-N

127 PGEX2T Vector

128 gata2fpyth

129 pGFP

Gata1promoter


come


1


factor Not in

xenbase

Gata1promoter


come


1


factor Not in

xenbase


comeXfli, fli, fli-1, Xfli1, Xfli-1


Not in xenbase


comeXfli, fli, fli-1, Xfli1, Xfli-1


Not in xenbase


comeNot in

xenbase


come



factor Not in

xenbase


comeFluorescent

ProteinNot in

xenbase

136 G2fTeasy

137 cardiac actin

140 Xolloid xolloid, Xld

141 pMX363

143 Xhox3 Xhox3, eve1

144

145 hGATA6MYQ


come



factor Not in

xenbase


come

cardiac actin, actc, cardiac alpha actin

cytoskeletal constituent

Not in xenbase


comemetalloprotea

se Not in

xenbase


come


XSnail2


l repressor Not in

xenbase


come

homeodomain


Not in xenbase

glucocorticoid receptor


come

xGR, gcr, gccr, grl,


steroid hormone receptor

Not in xenbase


come



factor Not in

xenbase

146 hGATA6MALT

147 pBUT2En-MT

168 sp64TGRHA

170 ENG-N Vector

171

180 pdx-1

181 HIG2fENG


come



factor Not in

xenbase


comeNot in

xenbase


come

xGR, gcr, gccr, grl,



Not in xenbase


comeNot in

xenbase

sp64TXbraGRHA


come

Xbra, X-bra, bra,

Xbrachyury, brachyury, ntl

T-box transcription

factor Not in

xenbase


come

ipf1, XlHbox8, pdx-

1

homeodomai

n transcription

factor

Not in xenbase


come



factor Not in

xenbase

182 G2fENG

183 pXA-1 XA

184 XeX-GM2

185

186

187

188 p200CAT


come



factor Not in

xenbase


come

Actin regulatory

protein (Wiskott-Aldrich

syndrome protein)

Not in xenbase


come


alpha-2


Not in xenbase

Gata1-(5/3)-GM2


come


1


factor Not in

xenbase

pMT7-rHNF4.wt


comeHNF-4-alpha, hnf4, nr2a1

steroid hormone receptor,


Not in xenbase

pMT7-rHNF4.N45C3

74


comeHNF-4-alpha, hnf4, nr2a1

steroid hormone receptor,


Not in xenbase


come


1


factor Not in

xenbase

189 G2fGST

190 G2GFP

191 pBSKS+ERtm

192

108 acvr2b

149 pbut3 Vector

150 pbut3enMT Vector


come



factor Not in

xenbase


come



factor Not in

xenbase


comeNot in

xenbase

PbsKS+ERtm (bx3)


comeNot in

xenbase

activin receptor

XB-CLONE-12271642


comeNot in

xenbase


comeNot in

xenbaseusto be XB-CLONE-8596638

154 Vector

158 pbut3mGR Vector

160 Clone 8 Vector

194 lac z

197 pSP64T/ET Vector

198 pSP64T/MET Vector

206 bUT2 Vector

psp64T n/s lacZ


comeNot in

xenbase


comeNot in

xenbase


comeNot in

xenbase


comeBeta-

galactosidase Not in

xenbase


comeNot in

xenbase


comeNot in

xenbase


comeNot in

xenbase

usto be XB-CLONE-8596638

207 bUT2-EnMT Vector

208 bUT2-VP16 Vector

209 bUT2-MT Vector

210 Vector

213 TK beta gal

214 XG1

215 pFT1A


comeNot in

xenbase


comeNot in

xenbase


comeNot in

xenbase

bUT2-VP16DXba


comeNot in

xenbase


comeBeta-

galactosidase Not in

xenbase


come


1


factor Not in

xenbase


come


1


factor Not in

xenbase


D135


216 Vector

217 pXbd-81CAT Vector

218 Vector

219 EF1a

220 XaT5globin

221 pSP64T Vector

222 p45but2 Vector

pXbetad-71CAT


comeNot in

xenbase


comeNot in

xenbase

XbLS42/33CATEO+


comeNot in

xenbase


come


alpha-2


Not in xenbase


come

hemoglobin, oxygen

transport Not in

xenbase


comeNot in

xenbase


comeNot in

xenbase

225 XnkxRVH

226 pXEX Vector

245 pId6

247 pParaxis

249 pZE7

264 pSP64TmBA growth factor

265


come

homeodomain


Not in xenbase


comeNot in

xenbase


comeNot in

xenbase


come

par-1, Par-1A, par1,

emk-2, ctak1, c-tak1

serine/threonine

kinase Not in

xenbase


come

thyl2, thylacine 2, thylacine2, hen2, thyl1, thylacine 1, thylacine1


Not in xenbase


comeNot in

xenbase

pC3XV3(fs) - Xvex-1


come

Xvex-1, vex1, ventral homeobox 1

homeodomai

n transcription

factor

Not in xenbase

268 XGATA1A

269 pds red

279 tal1

281 pBS-Cerberus

282 pRL1-null

283 Elf1 Elf-1, xelf1

284 pbUT2-Flag Vector


come


1


factor Not in

xenbase


comeFluorescent

ProteinNot in

xenbase


come

SCL, tal-1, bHLHa17,

tcl5, stem cell leukaemia

blood/vascular TF

Not in xenbase


come

xcer, xcer-1, xcer1, cer, Cerberus, tCerberus,

cer-1, dand4

secreted BMP antagonist

Not in xenbase


comeNot in

xenbase


cometranscription

factorNot in

xenbase


comeNot in

xenbase

286 PRL1TK

287 prl1CMV

289 pBUT2-HA Vector

290 Vector

293 Vector

294 pVPFli

296 pXMLC2


comeNot in

xenbase


comeNot in

xenbase


comeNot in

xenbase

pbUT2-En-Flag


comeNot in

xenbase

Fli-VP16-pcDNA3


comeNot in

xenbase


comeNot in

xenbase


come XMLC2,

mlc2, cmh10

Myosin regulatory light chain,

EF-Hand protein

superfamily

Not in xenbase

297 G6ERpcDNA3

316

317 VegT (Brat)

327 pBUT2-En-HA Vector

330 aSCL-GR-EN Vector

331 Vector

332 Vector


come



factor Not in

xenbase

sizzled 2 (frzb3)


come


CRD domain wnt inhibitor

Not in xenbase


come

brat, xombi, antipodean, tVegT, Apod

T-box transcription factor, meso-endodermal determinant

Not in xenbase


comeNot in

xenbase


comeNot in

xenbase

pBUT_flagRFP (not red

fluorescent protein)


comeNot in

xenbase

pCS2-n beta gal ITR


comeNot in

xenbase

391 aplnr

393

394 ODC

395 sox17

396 xnr2

397 LFABP

428 zCRP2

apelin receptor

apj, apjr, hg11, X-msr, msr, Xmsr,

angio1, Xangio1,

agtrl1


XB-CLONE-12271664

Not in xenbase

metabolic enzyme

Not in xenbase

endodermal TF

Not in xenbase


Not in xenbase

fatty acid binding

Not in xenbase


comecrp2, lmo5,

smlim crp2, lmo5,

smlimNot in

xenbase

429 zCalponin XclpH3, clpH3 Calponin

430 zSmoothelin n/a

431 pBut3HA Vector

433 iFGF-R1

435 FrzB1 pCS2

440 pCS2 Vector

447 PSP73 XmyoD


comeNot in

xenbase


come

Calponin homology domain

Not in xenbase


comeNot in

xenbase


come

HBGF-1, aFGF, acidic

fgf, fgf-1

heparin-binding


xenbase


come

Frzb-1, xfrzb, fre, os1, fzrb,

fiz, frp-3, frzb1, sfrp3, srfp3, frzb-

pen


Not in xenbase


comeNot in

xenbase


come

myogenic factor 3, MyoD,

XtmyoD, XMyoD, mf25

myogenic helix-loop-

helix transcription

factor

Not in xenbase

452

453

455 pEN-zfSCL Vector

465

469 Vector

470 Vector

HW-42

XGata5A (September

03)


come

xGATA-5, xGATA5,


GATA-5, gata-4a


factor Not in

xenbase

XGata5B (September

03)


come

xGATA-5, xGATA5,


GATA-5, gata-4a


factor Not in

xenbase


comeNot in

xenbase

D PBS-Stbm (Stbm Mutant

4)


come

tb1, Strabismus, lpp1, ltap,

stbm, stbm1, Van Gogh,

vang-like 2

Non-canonical wnt

pathway signaling protein

Not in xenbase

pCS2-delta StuI-SnaBI


comeNot in

xenbase

pCS2-En-HA delta StuI-

SnaBI


comeNot in

xenbase

endoderm promoter/fluo

rescent protein

HW-43

HW-44

HW-45

HW-46

HW-47

HW-48

HW-49 TF


rescent protein


rescent protein


rescent protein


rescent protein


rescent protein


rescent protein

HW-50

HW-51

HW-52

HW-53

HW-54

HW-55

HW-56


rescent protein


rescent protein


rescent protein

endoderm promoter/luci

ferase


rescent protein


rescent protein


rescent protein

HW-57 receptor

HW-58 receptor

HW-59 RNA binding

HW-60 RNA binding

HW-61 RNA binding

HW-62

HW-63

vegetal localised

vegetal localised

HW-64

HW-65

HW-66

HW-67

HW-68 RNA binding

HW-69 RNA binding

HW-70 RNA binding

vegetal localised

vegetal localised

vegetal localised

vegetal localised

HW-71

HW-72

HW-73

HW-74

HW-75

HW-76

HW-77

vegetal localised

vegetal localised

vegetal localised

vegetal localised

vegetal localised

vegetal localised

vegetal localised

HW-78

HW-79

HW-80

HW-81

HW-82 RNA binding

HW-83 RNA binding

HW-84 RNA binding

vegetal localised

vegetal localised

vegetal localised

vegetal localised

HW-85

HW-86

HW-87

HW-88 RNA binding

HW-89 RNA binding

HW-90 RNA binding

HW-91

centriolar binding

vegetal localised

vegetal localised

centriolar binding

HAR-194

HAR-229 TF

1676 Cugbp1b

1677 Zfp423

1679 Tankyrase2 tankyrase-2 Ankyrin

1680 Rasip1

signalling protein


come

eden-bp, embryo

deadenylation element

binding protein,

edenbp, cug-bp, cug-bp1,

cugbp, nab50,

brunol2, cugbp1

mediation of sequence-

specific mRNA

deadenylation

Not in xenbase


come

oaz, ebfaz, roaz, zfp423,

zfp104 zinc finger, C2H2 type

Not in xenbase


comeNot in

xenbase


come

rasip1 [Predicted: Source is Human

Entrez Gene]

Myosin class V heavy chain

Not in xenbase

1681 Lyl1 n/a

1682 Sfrp1a

1683 Ankrd11 n/a

1684 Elf3 n/a

1685 Klf13 n/a

1686 Zfat

1687


come transcription

factor Not in

xenbase


come

frzA, frp, frp1, frp-1,

sarp2, XfrzA modulator of Wnt signaling

Not in xenbase


come

Projectin/twitchin and

related proteins

Not in xenbase


come transcription

factor Not in

xenbase


comezinc finger, C2H2 type

Not in xenbase


come zfat1, ZFAT zinc finger 1

zinc finger, C2H2 type

Not in xenbase

pCS2+GR-HA-LefDN-βCTA


comexlef-1, lef-1,

xlef1

HMG box transcription factor, wnt signaling

Not in xenbase

1688

1689

1692

1693 Su(H)1

1694 Aadat-like

1695 Meis3 XMeis3, mrg2

1696 Arnt2 mgc53123

pT7TS-HA-GR-dNXTcf3


come

e2a, itf1, tcf-3, xtcf3, xtcf-

3, vdir, bhlhb21, tcfe2a

helix-loop-helix


Not in xenbase

p-6.0Kb Hhex:GFP


come XHex, tHex,

hex

homeodomai

n transcription factor and

Fluorescent Protein

Not in xenbase

Esr1-pGemT Easy


come

ERalpha, ERbeta,

xlERalpha1, xlERalpha2, XER, nr3a1,

xesr-1, xesr1


Not in xenbase


come

X-Su(H), XSu(H), Su(H),

suppressor of hairless,

rbpsuh, CBF1

signal transduction/

notch signaling pathway

Not in xenbase


come

aminoadipate aminotransfer

ase, kat2, katii

Aromatic amino acid

aminotransferase and related

proteins

Not in xenbase


come

homeodomai

n transcription

factor

Not in xenbase


come transcription

factor Not in

xenbase

1698 Lemd3

1699 Xpc

1700 Erf n/a

1701 Stc1 n/a

1702 CyclinG1-like n/a n/a

1703 Crp2

1704 Dll2


come

man1, xman1, sane,

Smad1 antagonistic

effector

TGF-beta/BMP

signaling Not in

xenbase


come xp3, rad4,

xpcc

Nucleotide excision repair

complex XPC-HR23B, subunit

XPC/DPB11

Not in xenbase


come transcription

factor Not in

xenbase


comestanniocalcin-

1Not in

xenbase


comeNot in

xenbase


comecrp2, lmo5,

smlim regulatory

protein Not in

xenbase


come X-dll2, tes1,

tes-1

homeodomai

n and LIM zinc binding

domain transcription

factor

Not in xenbase

XB-CLONE-11536601

1705 Dll1

1706 MespB mesp1, mesp

1707 VegfA-like vegf, vegf-a

1708 VegfC

1709 Aly

1711 Xcdh1 chd1

1718 Hesr2


come

X-delta-1, delta1, delta-1, Xdelta-1, XDelta1, x-delta, Delta-1, Xdelta1



Not in xenbase


come transcription

factor Not in

xenbase


comepeptide


xenbase


comevegf-c, xvegf-

c peptide


xenbase


come

no gene associated with this

clone

Not in xenbase


come

Chromodomai

n-helicase DNA-binding

protein

Not in xenbase


comehesr2,

gridlock

helix-loop-helix

transcription factor/transcri

ptional repressor

Not in xenbase

XB-CLONE-11536601

1719 hoxc10

1720 foxf1

1721 hand1

1747 iASPP-GFP ppp1r13l

1748 ASPP2-GFP

1751 ASPP1-GFP

1756 Hes3.3 esr2


comemgc80182, XHoxc10

homeodomain


Not in xenbase


come


forkhead domain


Not in xenbase


come eHand, hand-

1

basic helix-loop-helix


Not in xenbase


come

p53-interacting

protein 53BP/ASPP,

contains ankyrin and

SH3 domains

Not in xenbase


come


clone

Not in xenbase


come


clone

Not in xenbase


come

helix-loop-helix


ptional repressor

Not in xenbase

XB-CLONE-11536601

1757 Hes6.1

1758 esr3

1782 esr1b

1783 hes1b

1785

1786 esr1-GFP

1787


come XHes6, Xhes-

6

helix-loop-helix


ptional repressor

Not in xenbase


come

bHLHb38, esr7, esr-7,

esr3

helix-loop-helix


ptional repressor

Not in xenbase


come

ERalpha, ERbeta,


xesr-1, xesr1


Not in xenbase


come hairy1, Xhairy1

helix-loop-helix


ptional repressor

Not in xenbase

esr1-pDonor221


come

ERalpha, ERbeta,


xesr-1, xesr1


Not in xenbase


come

ERalpha, ERbeta,


xesr-1, xesr1

steroid hormone

receptor and Fluorescent

Protein

Not in xenbase

esr7-pDonor221


come


esr3

helix-loop-helix


ptional repressor

Not in xenbase

XB-CLONE-11536601

1788 esr7-GFP

367 PSP64T-Bix4

498 pBut3GW Vector

499 pBut2HAGW Vector

500 Vector

501 Vector

504 pCSGFP2GW Vector


come


esr3

helix-loop-helix


ptional repressor and Fluorescent

Protein

Not in xenbase


comeNot in

xenbase


comeNot in

xenbase


comeNot in

xenbase

pBut2-en-HAGW


comeNot in

xenbase

pBut2-VP16(delta

Xba)GW


comeNot in

xenbase


comeNot in

xenbase

XB-CLONE-11536601

D298

XB-CLONE-11536601

505 pBut3HAGW Vector

507 pHSP Vector

932 MafbGW

933 MafbHA

934 Mafbeng

935 MafbVp16

936 MafbGFP


comeNot in

xenbase


comeNot in

xenbase


come

kreisler, xmafb,

valentino

basic leucine zipper (bZIP) transcription

factor

Not in xenbase


come

kreisler, xmafb,

valentino


factor

Not in xenbase


come

kreisler, xmafb,

valentino


factor

Not in xenbase


come

kreisler, xmafb,

valentino


factor

Not in xenbase


come

kreisler, xmafb,

valentino


factor

Not in xenbase

937 sMaf2GW c-maf, xmaf

938 sMaf2HA c-maf, xmaf

939 sMaf2eng c-maf, xmaf

940 sMaf2Vp16 c-maf, xmaf

941 sMaf2GFP c-maf, xmaf

942 Klf6GW Zn-finger

943 Klf6eng Zn-finger


comeregulation of transcription

Not in xenbase



Not in xenbase



Not in xenbase



Not in xenbase



Not in xenbase


come

gbf, zf9, bcd1, cba1, cpbp, pac1, st12, copeb

Not in xenbase


come


Not in xenbase

944 Klf6Vp16 Zn-finger

945 Klf6GFP Zn-finger

946 SimKlf6 Zn-finger

947 OAZ

948 Nrl ?

968 GR-Su(H)DBM

969 DeltaStu


come


Not in xenbase


come


Not in xenbase


come


Not in xenbase


come



Not in xenbase


come

rp27, nrl-maf, l-maf,

XL-maf, xlmaf Not in

xenbase


come

dbm, dopamine

beta-monooxygen

ase, dopamine

beta-hydroxylase, dopamine-

beta-hydroxylase

Dopamine beta-

monooxygenase

Not in xenbase


come




Not in xenbase

970

971 STAT3-GR

972 Fev n/a

973 Xkl-1

974 CRLR

975 Twist1b

976 Ramp2

Hesr1/HRT1-MT-HGR-MOD


come

hrt1, XHRT1, chf2, hrt-1,

hesr1, herp2, oaf1, bc8

helix-loop-helix


ptional repressor

Not in xenbase


come Xstat3, aprf,

hies intracellular

signaling Not in

xenbase


come transcription

factorNot in

xenbase


come

Xsl, Xsl-1, Xsl-2, Xkl-1,

steel

ligand for the kit tyrosine

kinaseNot in

xenbase


comeNot in

xenbase


comeXtwi, X-twi, twist, Xtwist


Not in xenbase


comedct1, dmt1,

nramp2 transmembra

ne solute exchange

Not in xenbase

977 Sip1

978 VEGF-D

979 PPARg

980 E-Cad cell adhesion

981 cMycI

982 EEN

983 Abcg2 bcrp


comecasp11, sip1,

srrp129Not in

xenbase


come vegfd, vegf-d endothelial


xenbase


come

PPAR gamma 2, PPAR gamma, xPPAR

gamma, xPPAR-gamma

nuclear transcription

factor Not in

xenbase


come

uvo, cdhe, ecad, lcam,

arc-1, cd324, e-cadherin, uvomorulin

Not in xenbase


come

c-myc, cmyc, Xcmyc, X-

cmyc transcription

factor Not in

xenbase


come

SH3-domain GRB2-like 1

Matched synonym for

human ortholog

Lysophosphat

idic acid acyltransfera

se endophilin/SH3GL, involved

in synaptic vesicle

formation

Not in xenbase


come

White subfamily. Involved in cholesterol

and phospholipid

transport

Not in xenbase

984 Rspo1 n/a

986 CD51 n/a Trypsin

987 p22Phox

1007 Smad7-tevGR xsmad7

1008 TEV-GR

1009 DLL1

1013 ESR4 n/a


come

Subtilisin-like proprotein

convertase Not in

xenbase


comeNot in

xenbase


comeNot in

xenbase


come

signal transduction,

TGF-beta pathway

Not in xenbase


comeNot in

xenbase


come




Not in xenbase


come

Protein transporter of

the TRAM (translocating

chain-associating membrane) superfamily

Not in xenbase

1014 ESR5

1015 Hesr1/Hey1

1018

1019 cMycII

1020

1025 Smad6

1026 TGFbR1


comeesr5, x-esr5,

Xesr5 hairy class


Not in xenbase


come



helix-loop-helix


ptional repressor

Not in xenbase

BMPRIA / ALK3


come

alk3, alk-3, skr5, cd292,

acvrlk3

serine/threonine

kinaseNot in

xenbase


come


cmyc transcription

factor Not in

xenbase

BMPRIA / ALK3


come

alk3, alk-3, skr5, cd292,

acvrlk3

serine/threonine

kinaseNot in

xenbase


come madh6, Xsmad6


TGF-beta pathway

Not in xenbase


come

aat5, alk5, skr4, alk-5,

lds1a, lds2a, tgfr-1, acvrlk4

serine/threoni

ne kinaseNot in

xenbase

1030 Csflr

1031 Lyve-1 like n/a

1033 Notch2 ags2

1035 Aiolos

1036 Pegasus

1037 MPO-A

1038 Ran small GTPase


comeNot in

xenbase


come

Proteins containing

Ca2+-binding EGF-like domains

Not in xenbase


come regulation of developmen

Not in xenbase


come aio, aiolos,

znfn1a3 zinc finger, C2H2 type

Not in xenbase


come pegasus, znfn1a5


Not in xenbase


come

mpo-A, xpox2,

myeloperoxidase, pmr1,

pmr-1, pox2

Peroxidase/oxygenase

Not in xenbase


come ran-1, tc4, gsp1, ara24

Not in xenbase

1039 TGFb2-A

1042 Msx2A Hhox 7.1

1045 Msx1

1046 Stripy

1047 DNER unq26

1048 FHL1 n/a

1049 FHL5 n/a


come

TGF-beta 2, tgf-beta 2, tgf-beta2

growth factor Not in xenbase


come

homeodomain


Not in xenbase


come

hox7, hox-7, msx-1, Xmsx-

1, xmsx1, Xhox-7.1

homeodomai

n transcription

factor

Not in xenbase


come ledgerline,

stripyNot in

xenbase


come calcium ion

binding Not in

xenbase


come

Focal adhesion protein

PINCH-1, contains LIM

domains

Not in xenbase


come

Regulatory protein MLP and related

LIM proteins

Not in xenbase

1050 HoxD8

1051 Hzf n/a

1052 HoxD4 n/a

1053 Tanabin

1054

1055 Musashi1 RNA binding

1056 LSP1-like lsp1


come hoxd-8, hox5.4

homeodomai

n transcription

factor

Not in xenbase


come

beta-1,6-N-acetylglucosaminyltransferase, contains WSC domain

Not in xenbase


come

homeodomai

n transcription

factor

Not in xenbase


come tanabin,

nestin intermediate

filament Not in

xenbase

RAB-activating


come


clone

Not in xenbase


come

Msi1h, nrp-1B, Musashi, Musashi-1, Musashi 1, Musashi1,

XNrp-1

Not in xenbase


come

7 transmembrane receptor

Not in xenbase

1057 Helios

1058 CD166-like

1059 ABCG2 bcrp

1060 CD41-like1

1061 CD166 cell adhesion

1062 VGlut1 bnpi, vglut1

1176 Id1


come

helios, znf1a2,

znfn1a2 zinc finger, C2H2 type

Not in xenbase


come


clone

Not in xenbase


come

White subfamily. Involved in cholesterol

and phospholipid

transport

Not in xenbase


come

cd41, cd41b, gp2b, gpiib, gta, hpa3

vitronectin receptor,

alpha subunit Not in

xenbase


come cd166, DM-

GRASPNot in

xenbase


come

Permease of the major facilitator

superfamily

Not in xenbase


come id3, xid3,

heir-1, xidia


Not in xenbase

1177 SMP30

1178 PDZK xpdzk1 PDZ domain

1179 cRet

1180 Cox1

1181 CD44

1182 ESR7

1183 BMP7


come

xsmp-30, smp30, xsmp30,

regucalcin

gluconolactonase

Not in xenbase


comeNot in

xenbase


come cret, c-ret, X-

ret, xret

tyrosine kinase

receptor for GDNF ligand

Not in xenbase


come

cox-1, mtco1, mt-

co1electron

transport Not in

xenbase


come

XCD44, lhr, mc56, mdu2, mdu3, mic4, pgp1, cdw44, cspg8, hcell,

mutch-i, ecmr-iii

cell surface glycoprotein

Not in xenbase


come


esr3

helix-loop-helix


ptional repressor

Not in xenbase


come bmp7, bmp-

7, op-1


growth factor

Not in xenbase

1184 ESR6e esr6e, esr-6e

1185 Rac1

1186 WT1a xwt1, wt-1

1187 Nrarp n/a

1188 Rac2 n/a

1189 Sema4c n/a Semaphorins

1190 Dusp5 n/a


come

helix-loop-helix


ptional repressor

Not in xenbase


comerac, mig5, tc-25, p21-rac1

small GTPase, Rho

typeNot in

xenbase


come

zinc finger transcription and splicing

factor

Not in xenbase


come

Myosin phosphatase,

regulatory subunit

Not in xenbase


comesmall GTPase,

Rho type Not in

xenbase


comeNot in

xenbase


comeMAP kinase

phosphatase Not in

xenbase

1191 ESR10A n/a n/a

1192 Stat1 XStat1

1193 LSP1 n/a

1194 MCL1-like

1195 Igsf11-like n/a

1196 Lurp n/a

1197 VegfD


comeNot in

xenbase


come intracellular

signaling Not in

xenbase


come


Not in xenbase


come


clone

Not in xenbase


come

Immunoglobu

lin C-2 Type/fibronec

tin type III domains

Not in xenbase


come XLURP-1,

lurp1 Not in

xenbase


come vegfd, vegf-d endothelial


xenbase

1198 Myef2 n/a

1199 Parp12 Ankyrin

1200 Mdg1 mgc53411

1201 Prom1

1202 ED1 cwf15

1203 NFE2-L2

1204 CCL19-like


come nucleic acid

binding Not in

xenbase


come

zc3h1, mst109, mstp109, zc3hdc1

Not in xenbase


comemolecular chaperone

Not in xenbase


come

prominin, rp41, ac133,

cd133, mcdr2, stgd4,

cord12, proml1

polytopic membrane

protein Not in

xenbase


come

Uncharacterized conserved

protein Not in

xenbase


come


clone

Not in xenbase


come


clone

Not in xenbase

1205 C3 n/a

1206 Scoc scoco

1207 MEKKK5 map4k5

1208 F13a f13a1 n/a

1209 CMKLR1-like

1210 Evi1

1211 SM22a Calponin


comeactivation of complement

Not in xenbase


come

Predicted coiled-coil protein

Not in xenbase


come

Mitogen-activated protein

kinase kinase kinase kinase

(MAP4K), germinal

center kinase family

Not in xenbase


comeNot in

xenbase


come


clone

Not in xenbase


come

evi-1, xEvi-1, evi1,

ecotropic viral

integration site 1


Not in xenbase


comemgc107855,

SM22Not in

xenbase

1212 KUP zbtb25

1213 CRTAM n/a

1214 CD41-like2 cd41

1215 MLLT4 af6, af-6

1216 Sema5B

1217 Lcp1-like

1218 HoxB7A



Not in xenbase


come

N-methyltransfe

raseNot in

xenbase


come



xenbase


comeactin filament

bindingNot in

xenbase


come

semg, semag, semaphorin-

5B signal

transduction Not in

xenbase


come

lpl, l-plastin, cp64, pls2,

lc64p

Ca2+-binding actin-

bundling protein

(fimbrin/plastin), EF-Hand

protein superfamily

Not in xenbase


come

hox2, hox2c, hho.c1, hox-2.3, XlHbox2

homeodomain


Not in xenbase

1219 ARNTL

1220 DLL4

1221 EPAS1

1222 TGIF cytokine

1223 Meis1 XMeis1

1224 HoxC5 XlHbox5

1225 CD97 tm7ln1


come bmal1, xBmal1


Not in xenbase


comexdll4, delta 2,

x-dll4



Not in xenbase


come

hif2alpha, mop2, ecyt4, hif2a, pasd2, bhlhe73, hif2,

hlf

transcriptiona

l regulator Not in

xenbase


comecsif, tgif, il-10, il10a

Not in xenbase


come

homeodomain


Not in xenbase


come

homeodomai

n transcription

factor

Not in xenbase


come

G protein-coupled

receptors Not in

xenbase

1226 Mel1 mel1

1227 Frataxin n/a

1228 NFE2L2 NF-E2R2

1229 PTPRM

1230 HPK1 n/a

1231 Hbb1

1232 Hbb2 hbb2


come

homeodomain


Not in xenbase


come

frataxin [Predicted: Source is Human

Entrez Gene]

Not in xenbase


come


factor

Not in xenbase


come

rptpm, rptpu, ptprl1, r-ptpu,

p-ptp-mu

Protein tyrosine

phosphatase Not in

xenbase


come

Mitogen-activated protein

kinase kinase kinase kinase

(MAP4K), germinal

center kinase family

Not in xenbase


come

Beta-1-globin, hbb1, hbga, hbgr, hsggl1, beta-globin

Globins and related

hemoproteins Not in

xenbase


come

Globins and related

hemoproteins Not in

xenbase

1233 HMOX1 n/a

1234 TFPT n/a n/a

1235 Hes1a

1236 TINP1

1237 AA4


1239 BOIP boip, XBOIP


come Heme

oxygenase Not in

xenbase


comeNot in

xenbase


comehairy1,

Xhairy1

helix-loop-helix


ptional repressor

Not in xenbase


come

tinp1, hclg1, yr-29,

cdk105, hcl-g1, hussy29,

hussy-29


protein related to ribosomal

protein S8E

Not in xenbase


come

c1qr1, c1qrp, cdw93, ecsm3, mxra4,

c1qr(p), c1qr, AA4

Fibrillins and related proteins

containing Ca2+-binding

EGF-like domains

Not in xenbase


come transcription

factor Not in

xenbase


come


protein Not in

xenbase

1240 Synbindin trappc4

1241 Coro1c hcrnn4

1242 Elfin

1243 Trem2 n/a n/a

1244 SPARC n/a

1245 Ednrb2 n/a

1246 Ednra


come

transport protein particle complex subunit

Not in xenbase


come

Actin-binding protein Coronin, contains WD40

repeats

Not in xenbase


come clim1, clp36, clp-36, elfin

Adaptor protein

Enigma and related PDZ-LIM proteins

Not in xenbase


comeNot in

xenbase


come

Matricellular protein

Osteonectin/SPARC/BM-40

Not in xenbase


come


Not in xenbase


come

eta, etar, etra,

Endothelin-1 receptor type

A

G protein-coupled receptor

Not in xenbase

1247 MLLT1 n/a

1248

1249 ZNF423

1250 Stmn1

1251 Evi1

1252 Clec2D clax, llt1, ocil C-type lectin

1253 Col3a1 n/a


come

Transcription initiation factor IIF, auxiliary subunit

Not in xenbase

DBM-Su(H)-GR


come

dbm, dopamine

beta-monooxygen

ase, dopamine

beta-hydroxylase, dopamine-

beta-hydroxylase

Dopamine beta-

monooxygenase

Not in xenbase


come



Not in xenbase


come

stathmin, oncoprotein 18, op18

intracellular signaling

Not in xenbase


come


ecotropic viral

integration site 1


Not in xenbase


comeNot in

xenbase


come

Collagens (type IV and

type XIII), and related

proteins

Not in xenbase

1254 NOMO2

1255 Ankyrin1 Ankyrin

1256 ED2 n/a

1257 Ets2b ets-2 n/a

1258 SpiC spi-c

1259 Notch2 ags2

1260 Elf2b mgc89405


come

Xnr-4, Xnr4, nr4, nodal4,

Nodal homolog 4

transforming growth factor

beta and bone


Not in xenbase


come ank, sph1,

sph2Not in

xenbase


come

Predicted membrane

proteinNot in

xenbase


comeNot in

xenbase


come

ETS transcription

factor Not in

xenbase


comeregulation of development

Not in xenbase


come transcription

factor Not in

xenbase

1261 Prg4 n/a

1262 ETV1 XER81, er81

1263 Efnb2a

1264 Src

1265 STAT6 n/a

1266 Gabpa-b

1267 STAT5B stat5 STAprotein


come

cacp, hapo, jcap, msf,

szp, lubricin Not in

xenbase


cometranscription

factorNot in

xenbase


comeephrinB2, ephrin B2

ligand for Eph receptor tyrosine kinase

Not in xenbase


come

c-src, src1, pp60v-src, pp60c-src, xsrc, src-1,

csrc

tyrosine kinase

Not in xenbase


come intracellular

signaling Not in

xenbase


come

XrpFI, X-alpha1, nft2, nrf2, nrf2a,

e4tf1a, e4tf1-60


Not in xenbase


comeNot in

xenbase

1268 Gabpa-a

1269 HoxD4 n/a

1270 Brd2-A

1271 Hzf-like

1272 ILK p59

1273 MCL1

1274 Mzf1 Zn-finger


come

XrpFI, X-alpha1, nft2, nrf2, nrf2a,

e4tf1a, e4tf1-60


Not in xenbase


come

homeodomai

n transcription

factor

Not in xenbase


come

ring3, fsh, nat, rnf3,

fsrg1

transcription initiation factor

Not in xenbase


come


clone

Not in xenbase


come Integrin-

linked kinaseNot in

xenbase


comemcl-1, mcl1l, mcl1s, bcl2l3

apoptosis regulator

Not in xenbase


come

mzf-1, mzf1b, znf42, zfp98,

zscan6 Not in

xenbase

1275 HoxD10

1276 HoxD8

1277 Ets1b n/a

1278 ETV3 mgc53585

1279 NFYA

1280 KLF11 n/a

1281 ETV3-like mgc53585


come

Xhoxd10, hox4, hox4d, hox4e, hox-

4.4

homeodomai

n transcription

factor

Not in xenbase


come hoxd-8, hox5.4

homeodomai

n transcription

factor

Not in xenbase


come ets-1, ewsr2,

xe1-a Not in

xenbase


cometranscription

factorNot in

xenbase


comenf-ya, hap2, cbf-a, cbf-b


Not in xenbase


come zinc finger, C2H2 type

Not in xenbase


come transcription

factor Not in

xenbase

1282 HoxA9

1283 HIF1AN n/a

1284 Gabarapl1

1285 Rap1a

1286 MLF2 n/a

1287 ALAS1 n/a

1288 EpoR n/a n/a


come

hoxa9-A, XlHbox6, XIHbox 6, XlHbox-6, xhoxa9

homeodomain


Not in xenbase


come


protein, contains JmjC

domain

Not in xenbase


comeatg8, gec1, apg8l, atg8l

Microtubule-associated

anchor protein

involved in autophagy

and membrane trafficking

Not in xenbase


come rap1, krev1,

krev-1Ras-related

GTPaseNot in

xenbase


come

Proliferation-related

protein MLF Not in

xenbase


come

5-aminolevulinate synthase

Not in xenbase


comeNot in

xenbase

1289 Elf1b Elf-1, xelf1

1299 Emr1 n/a

1300 NFkB1

1301 MTGR1

1302 Mef2c XMEF2C

1303 CD274 like

1304 CD164


come transcription

factor Not in

xenbase


come

G protein-coupled

receptors Not in

xenbase


come

kbf1, ebp-1, nfkb-p50, nf-kappaB, nfkb-

p105, nf-kappa-b

transcriptiona

l regulator Not in

xenbase


come MTGR1, XETOR

transcription factor,

expressed during

neurogenesis

Not in xenbase


come

MADS-box transcription

factor Not in

xenbase


come


clone

Not in xenbase


come


clone

Not in xenbase

1305 Globin

1306 Nr2f2

1307 Elf2 mgc89405

1308 Gabpb1

1309 cJun

1310 C/EBPd

1311 WASP


come


clone

Not in xenbase


come

arp1, svp40, couptfb, tfcoup2, couptfii,

xCOUP-TFB, xcouptfb


Not in xenbase


cometranscription

factor Not in

xenbase


come

e4tf1, gabpb, babpb2, e4tf1b, gabpb2, nrf2b1,

nrf2b2, e4tf1-47, e4tf1-53, xrpflbeta1, x-

beta 1-1b


Not in xenbase


come


clone

Not in xenbase


comecebpd, C/EBP

delta


factor

Not in xenbase


comethc, imd2, thc1, wasp

actin regulatory

protein Not in

xenbase

1315 PABP1

1316 Tagln2 Calponin

1317 CXCR2

1318 HERPUD2 n/a

1319 CDX2

1320 CDX4

1323 Tafazzin n/a


come

pab1, pabp, pabp1, pabpc2

Polyadenylate

-binding protein (RRM superfamily)

Not in xenbase


come

sm22-a, sm22-alpha, sm 22a, sm

22alpha

Not in xenbase


come

cxcr1 [Predicted: Source is Human

Entrez Gene]


Not in xenbase


come

membrane-associated ER

protein involved in

stress response

Not in xenbase


come cdx3-like,

xtcad1, xcad1

homeodomai

n transcription

factor

Not in xenbase


come

mgc68909, mgc130769, cad3, Xcad3, Xtcad3, Xcad-

3

homeodomain


Not in xenbase


come

Phosphate acyltransfera

se Not in

xenbase

1324 Deltex3

1325 PBX1

1327 MLLT4 af6, af-6

1328 Heg1-like n/a

1329 ALG13 LOC549862

1330 Seb4a

1331 Jumonji Jumonji, jmj DNA binding


come


clone

Not in xenbase


come

Xpbx1, Xpbx1b, pbx1b

homeodomain


Not in xenbase


comeactin filament

bindingNot in

xenbase


come

Mitochondrial

ribosomal protein L32

Not in xenbase


come

Predicted glycosyltransf

erase Not in

xenbase


come

seb4, seb4-a, rbm24-a,

xseb4, seb-4 nucleic acid

binding Not in

xenbase


comeNot in

xenbase

1332 Ets2a ets-2 n/a

1333 ERF-b n/a

1334 FAD49 sh3pxd2b

1335 Septin2

1336 Rnf41 flrf

1337 Marks-like

1338 Etsrp


comeNot in

xenbase


come transcription

factor Not in

xenbase


comeNADPH oxidase

Not in xenbase


come


clone

Not in xenbase


come

mediator of ubiquitin

ligase activity Not in

xenbase


come


clone

Not in xenbase


come er71,

etsrp71, Etsrp Predicted


Not in xenbase

1339 Esr9 esr9

1341 Del1

1342 CIR xcir, cir

1343 PBX2

1344 ZBTB44 n/a

1345 vWF

1346 FLVCR2 n/a


come

helix-loop-helix


Not in xenbase


comeDEL-1, Del1,

XDel1zinc

carboxypeptidase [KOG]

Not in xenbase


come

cbf1-interacting

corepressor Not in

xenbase


come hox12,

pbx2mhc

homeodomai

n transcription

factor

Not in xenbase



Not in xenbase


come

von Willebrand factor, vwd,

f8vwf

glycoprotein produced by

vascular endothelial

cells, required for

blood clotting

Not in xenbase


come

Permease of the major facilitator

superfamily

Not in xenbase

1347 Myst3

1348 Prohibitin2

1349 Myl9 n/a

1350 Elfin

1351 NFATC1

1352 Pegasus

1353 TCF4


come


clone

Not in xenbase


come

prohibitin 2, bap37, bcap37, xphb2,

prohibitin2

Prohibitin-like protein

Not in xenbase


come

Myosin regulatory light chain,

EF-Hand protein

superfamily

Not in xenbase


comeclim1, clp36, clp-36, elfin

Adaptor protein

Enigma and related PDZ-LIM proteins

Not in xenbase


come nfat2, nfatc,

nf-atc transcription

factor Not in

xenbase


comepegasus, znfn1a5


Not in xenbase


come tcf-4, Xtcf-4,

Xtcf4

helix-loop-helix (bHLH) transcription

factor

Not in xenbase

1354 Mdr abcb1

1355 Aqp1

1356 Livertine

1357 VETP ptprb

1358 Seb4b

1359 TCF3

1360 Cdk9a


come

Multidrug/pheromone

exporter, ABC superfamily

Not in xenbase


come

chip28, aquaporin-1, aquaporin 1, aquaporin1

integral membrane pore (water

channel) protein

Not in xenbase


come


clone

Not in xenbase


come

Protein tyrosine

phosphatase, contains fn3

domain

Not in xenbase


come

seb4, seb4-a, rbm24-a,

xseb4, seb-4 nucleic acid

binding Not in

xenbase


come


3, vdir, bhlhb21,

tcfe2a

helix-loop-helix


Not in xenbase


come

tak, c-2k, ctk1, cdc2l4,

pitalre

cyclin T-dependent

kinaseNot in

xenbase

1361 Flt3

1362 TCFe2a

1363 Cdk9b

1364 LysozymeC lyz

1375 Lypd5

1376 Bzrp

1379 Gse1 n/a


come flk2, stk1, flt-

3 serine/

threonine kinase

Not in xenbase


come



helix-loop-helix


Not in xenbase


come

tak, c-2k, ctk1, cdc2l4,

pitalre

cyclin T-dependent

kinaseNot in

xenbase


comeanti-microbial

agent Not in

xenbase


come


clone

Not in xenbase


come

dbi, ibp, mbr, pbr, bzrp, pkbs, ptbr, mdrc, pk18

Peripheral-type

benzodiazepine receptor and related

proteins

Not in xenbase


come

transcriptiona

l regulator Not in

xenbase

1380 Yes yes, c-yes

1381 DBAf2

1382 Cpne8 n/a Copine

1383 HIST1H1 LOC549623 Histone H4

1384 PDIp

1385 Prkar1a

1386 Ddx55 n/a


come

serine/threonine

kinase Not in

xenbase


come


clone

Not in xenbase


comeNot in

xenbase


comeNot in

xenbase


comepdi, XPDIp,

pdip

Protein disulfide

isomerase (prolyl 4-

hydroxylase beta subunit)

Not in xenbase


come

pkr1, cnc1, tse1, ppnad1,

prkar1

protein kinase

regulatory subunit

Not in xenbase


come

ATP-dependent

RNA helicase Not in

xenbase

1387 CD91

1388 VCAM1-like vcam1

1389 PSGL-like

1390 Cel

1391 b-Catenin

1392 MHC class II n/a

1393 HoxA3a


come

lrp, cd91, apr, a2mr, apoer,

tgfbr5, igfbp3r

alpha-2-macroglobulin endocytic

surface receptor

Not in xenbase


come

immunoglobu

lin Not in

xenbase


come


clone

Not in xenbase


come

xcel, carboxyl

ester lipase, fap, bsdl, bssl, fapp, lipa, cease,

mody8

Carboxylester

ase and related

proteins

Not in xenbase


come ctnnb1,

beta-catenin

adherens junction

protein/component of wnt

signaling pathway

Not in xenbase


come

hla-dqa1, LOC1001277

27Not in

xenbase


come

hoxa3a, hoxa-3, hox1e

homeodomain


Not in xenbase

1394 HoxC6 XlHbox1

1395 Podxl

1396 Nrlh3 nr1h2

1399 PP11

1400 Itga4 n/a

1401 CtssB cathepsin S

1402 PrkD1


come

homeodomai

n transcription

factor

Not in xenbase


come


clone

Not in xenbase


come


Not in xenbase


come

pp11, PRSS26,

xp11, xendou, p11,

endou-b, endou-c, endou-d

Placental protein 11

Not in xenbase


come

Vitronectin receptor,


xenbase


come

cysteine-type endopeptidas

e Not in

xenbase


come

pkd, pkcm, prkcm, pkc-mu, protein

kinase C, mu

serine/threonine

kinase Not in

xenbase

1403

1404 Smo-B

1405 FRL2 FRL2 n/a

1406 Hhip hip, Xhip

1407 NFE2L2-like NF-E2R2

1408 Zap70 n/a

1409 NFE2RF1-like n/a

MHC Class II D


come


clone

Not in xenbase


come

XSmo, X-smoothened, smoothened

hedgehog signal

transduction Not in

xenbase


comeNot in

xenbase


come

regulatory component of

hedgehog signaling pathway

Not in xenbase


come


factor

Not in xenbase


comeTyrosine kinases

Not in xenbase


come


factor

Not in xenbase

1410 Atp6v0b

1411 Nr4a1

1412 Sax

1413 BCL11B n/a

1414 Gp91-1 cybb

1415 HIF1AN n/a

1416 Lbh-like


come atp6f, hatpl,

vma16

Vacuolar H+-ATPase V0

sector, subunit c''

Not in xenbase


come

NGFI-B, hmr, n10, tr3,

np10, gfrp1, nak-1, ngf1b,

nur77

hormone receptor

Not in xenbase


come

alk2, alk-2, fop, skr1, tsri, actri, acvr1a, acvrlk2, sax,

alk8

activin receptor,

serine/threonine protein

kinase

Not in xenbase



Not in xenbase


comeelectron

transport Not in

xenbase


come


protein, contains JmjC

domain

Not in xenbase


come


clone

Not in xenbase

1417 Tbxas1

1418 FMNL2 fhod2

1419 Itgb3 cd61, gp3a

1420 HoxD1

1421 Aqp0-A

1422 Aqp1

1423 HoxD13 XHoxd13


come

txs, cyp5, thas, txas, cyp5a1, ghosal

Cytochrome P450

CYP3/CYP5/CYP6/CYP9

subfamilies

Not in xenbase


come Rac1 GTPase effector FRL

Not in xenbase


come

Integrin beta subunit (N-

terminal portion of

extracellular

Not in xenbase


come

xlab, mgc131028, xhox.lab1, Xhox.lab,

xhoxd1, Xlab-1

homeodomai

n transcription

factor

Not in xenbase


come aquaporin 0,

aqp0water

transporter (aquaporin)

Not in xenbase


come



channel) protein

Not in xenbase


come

homeodomai

n transcription

factor

Not in xenbase

1424 CCR2 n/a

1425 Aqp0-B

1426 Aqp3

1427 Aqp4 aquaporin 4

1428 HoxB6

1429 Gata2

1430 Socs3


come

cc-ckr-2, ccr2a, ccr2b, cd192, ckr2, ckr2a, ckr2b, cmkbr2, mcp-1-r, mcp-1r

Not in xenbase


come aquaporin 0,

aqp0water


Not in xenbase


come

AQP-x3, aqp-3, Aquaporin-3, aquaporin-

3b, aqp3b, aqp-3b


channel) protein

Not in xenbase


come


channel) protein

Not in xenbase


come hox2b, hox-

2.2

homeodomai

n transcription

factor

Not in xenbase


come



factor Not in

xenbase


come

xsocs3, cis3, ssi3, atod4, cish3, ssi-3,

socs-3

intracellular signaling

Not in xenbase

1431 CDX2

1432 HoxA4 hox1d

1433 Eomes

1434 CD9 n/a

1435 Nampt

1436 MDS029

1437 cFos c-fos, xfos


come cdx3-like,

xtcad1, xcad1

homeodomai

n transcription

factor

Not in xenbase


come

homeodomai

n transcription

factor

Not in xenbase


come

xeomes, eomesodermi

n

T-box transcription

factor Not in

xenbase


come

Tetraspanin family

integral membrane

protein

Not in xenbase


come

pbef1, pre-B-cell colony enhancing factor 1

pyridine nucleotide

biosynthesis Not in

xenbase


comecisd1, zcd1,

mitoneet

CDGSH-type Zn-finger

containing protein

Not in xenbase


come


factor

Not in xenbase

1438 BAMBI-A mgc89501

1439 FOG

1440 XBMP1

1442 ANF anf, anp

1443 ISLET1

1444 Vector

1445 Vector


come

negative regulator of

TGF-beta (pseudorecep

tor)

Not in xenbase


come

fog, friend of GATA, fog1, fog-1, Xfog


Not in xenbase


come

xtld, tld, tolloid, bmp-

1, xbmp1 metallopeptid

ase Not in

xenbase


come

regulation of blood

pressure and electrolyte balance

Not in xenbase


come

islet-1, Xisl-1, Xislet-1,

islet1

homeodomai

n transcription

factor

Not in xenbase

SCL gateway HA tagged


comeNot in

xenbase

Runx1 P1 gateway HA

tagged


comeNot in

xenbase

1446 Vector

1447 Flk-1

1448

1449

1450 Sfrs5

1451

1452

Runx1 P2 gateway HA

tagged


comeNot in

xenbase


come

flk1, flk-1, vegfr-2,

vegfr2, xflk-1


Not in xenbase

Xenopus SCL 96%


come


tcl5, stem cell transcription

factorNot in

xenbase

Xenopus SCL 84%


come



factorNot in

xenbase


come hrs, sfrs5,

srp40 alternative

splicing factor Not in

xenbase

Xenopus SCL HA tagged


come



factorNot in

xenbase

xenopus HA tagged Runx1

P1


come

Xaml, aml1 oncogene,

aml-1, Runx-1, aml1, aml, cbfa2, evi-1,

amlcr1, pebp2ab,

aml1-evi-1, Xaml1

transcriptiona

l regulatorNot in

xenbase

1453

1454 DSCR1

1461 Eos eos, znfn1a4

1462 Fli1-like

1463 Numb-like

1464 Tel2

1465 LFng

xenopus HA tagged Runx1

P2


come

Xaml, aml1 oncogene,

aml-1, Runx-1, aml1, aml, cbfa2, evi-1,

amlcr1, pebp2ab,

aml1-evi-1, Xaml1

transcriptiona

l regulatorNot in

xenbase


come

dscr1, Down syndrome

critical region gene 1,

adapt78, csp1, dsc1,

mcip1

calcineurin-mediated signaling pathway inhibitor

Not in xenbase



Not in xenbase


come Xfli, fli, fli-1, Xfli1, Xfli-1


Not in xenbase


come

nbl, cag3a, ctg3a, numbr,

numb-r, tnrc23,

numblike

Adaptor protein NUMB

Not in xenbase


cometel-2, tel2,

telb

Transcription factor NERF and related

proteins, contain ETS

domain

Not in xenbase


come lunatic fringe

glycosyltransf

erase, signaling molecule

Not in xenbase

1466 CEBPz n/a

1467 KLF10 n/a

1468 PBR

1469 Heg1 n/a n/a

1470 Pmpca n/a

1471 BCL11A n/a

1472 Pctk2


come

ribosomal subunit

biogenesis protein

(transcription factor)

Not in xenbase



Not in xenbase


come

dbi, ibp, mbr, pbr, bzrp, pkbs, ptbr, mdrc, pk18

Peripheral-type

benzodiazepine receptor and related

proteins

Not in xenbase


comeNot in

xenbase


come

Mitochondrial processing peptidase,

alpha subunit

Not in xenbase



Not in xenbase


come PCTAIRE2,

pctk2 serine/

threonine kinase

Not in xenbase

1473 PKAbeta

1474 Lbh xlcl2 n/a

1475 NR2F6

1476 Numb S171

1482 pCS2+ Vector

1489 VECAD cell adhesion

1490 Ikaros


come

XPKAbeta, p70 S6

kinase, pkacb

serine/threonine

kinase Not in

xenbase


comeNot in

xenbase


comeear2, ear-2,

erbal2 steroid

hormone receptor

Not in xenbase


come adaptor protein

Not in xenbase


comeNot in

xenbase


come

VE-cadherin, cadherin-5,

ve-cadNot in

xenbase


come

zinc finger protein,

subfamily 1A, 1, znfn1a1,

ik1, lyf1, ik-1, ikaros,

pro0758, znfn1a1, ika


Not in xenbase

1491 Notch3 casil, cadasil

1492 Fog

1493 Tie1 n/a

1494 Tie2

1496 ESR1-GFP

1497 HESR1-GFP

1501 Aspp1


come



EGF-like domains

Not in xenbase


come



Not in xenbase


come

serine/threoni

ne kinase Not in

xenbase


come tie2, tie-2,

Xtie2endothelial

cell receptor Not in

xenbase


come

ERalpha, ERbeta,


xesr-1, xesr1

steroid hormone

receptor and Fluorescent

Protein

Not in xenbase


come



helix-loop-helix



Protein

Not in xenbase


come


clone

Not in xenbase

1502 Aspp2

1503 iAspp ppp1r13l

1504 Val1 n/a

1505 MLP MLP, CRP3

1506 Wnt4

1507 RPL10a n/a

1508 ELT


come


clone

Not in xenbase


come

p53-interacting

protein 53BP/ASPP,

contains ankyrin and

SH3 domains

Not in xenbase


come

val [Predicted: Source is Human

Entrez Gene]

Not in xenbase


come

Regulatory protein MLP and related

LIM proteins

Not in xenbase


come Xwnt4, Xwnt-

4, wnt-4

secreted growth


regulator

Not in xenbase


come

60S ribosomal

protein L10A Not in

xenbase


come

eltd1 [Predicted: Source is Human

Entrez Gene]


Not in xenbase

1509 Pdcd2 n/a

1510 Nlk2

1511 Lphn2 n/a

1512 Ensa

1513 Crabp2 xCRABP

1514 C9 n/a Semaphorins

1515 Runx2


come

mynd type zinc finger

protein, regulator of

cell death/apopto

sis

Not in xenbase


come klf5, cklf, iklf, bteb2


Not in xenbase


come

G protein-coupled

receptors Not in

xenbase


come

endosulfine alpha, alpha-endosulfine,

arpp-19e

Regulator of ATP-sensitive K+ channels

Alpha-endosulfine/ARPP-19 and

related cAMP-regulated

phosphoproteins

Not in xenbase


comefatty acid binding

Not in xenbase


comeNot in

xenbase


come

ccd, aml3, ccd1, osf2,

cbfa1, pea2aa,

pebp2a1, pebp2a2, pebp2aa, pebp2aa1

transcriptiona

l regulator Not in

xenbase

1524 Egr4 n/a

1525 SBNO1

1526 DLL1

1527 Flt4

1528 Lbh xlcl2 n/a

1529 Flt4

1530 Prdx6



Not in xenbase


come strawberry

notch nuclear

helicase Not in

xenbase


come




Not in xenbase


comevegfr3, vegfr-

3, flt-4


growth factor receptor

Not in xenbase


comeNot in

xenbase


comevegfr3, vegfr-

3, flt-4


growth factor receptor

Not in xenbase


come

prx6, aop2, peroxiredoxin

6, prx-6

Alkyl hydroperoxide reductase, thiol specific antioxidant and related enzymes

Not in xenbase

1531 Mafk n/a

1532 Coro1c hcrnn4

1533 Irx3

1534 HoxC8

1535 Ankrd1 CARP

1536 Kitlg

1537 MMP9


come


factor

Not in xenbase


come

Actin-binding protein Coronin, contains WD40

repeats

Not in xenbase


come


homeodomai

n transcription

factor

Not in xenbase


come xhoxc8, hoxc-8

homeodomai

n transcription

factor

Not in xenbase


come Ankyrin repeat

Not in xenbase


come

Xsl, Xsl-1, Xsl-2, Xkl-1,

steel

ligand for the kit tyrosine

kinaseNot in

xenbase


come

MMP-9, gelb, clg4b,

mandp2

extracellular matrix

metalloprotease

Not in xenbase

1538 Notch2 ags2

1539 Cisd2

1541 Aqua-0

1542 tPA Trypsin

1543 MafB

1544 Nrl n/a


come regulation of development

Not in xenbase


comeeris, wfs2,

zcd2, miner1

CDGSH-type Zn-finger

containing protein

Not in xenbase


comeaquaporin 0,

aqp0 water


Not in xenbase


come

plat [Predicted: Source is Human

Entrez Gene]

Not in xenbase


come

kreisler, xmafb,

valentino


factor

Not in xenbase


come

rp27, nrl-maf, l-maf,

XL-maf, xlmaf Not in

xenbase

1545 Flk1

1546 Band3

1547 Aly

1548 Irx4

1549 Fli1

1550 Karp1BP1

1551 cMaf c-maf, xmaf


come


vegfr2, xflk-1


Not in xenbase


come

ae1, bnd3, cd233,

empb3, epb3, rta1a, wd1,

band 3

Na+-independent

Cl/HCO3 exchanger AE1 and related

transporters (SLC4 family)

Not in xenbase


come

alyref [Predicted: Source is Human

Entrez Gene]

RRM motif-containing

protein Not in

xenbase


come iroquois-4, irxa3, Xiro4

homeodomai

n transcription

factor

Not in xenbase




Not in xenbase


come


clone

Not in xenbase


come regulation of transcription

Not in xenbase

1552 BTK n/a

1553 Ihh

1554

1555

1556

1557

1558


come

atk, bpk, imd1, agmx1,

psctk1, xlaNot in

xenbase


come

X-BHH, banded

hedgehog, bhh, X-hh,

indian hedgehog,

Xbhh

intercellular signaling

Not in xenbase

Aquaporin1 (AOPZ3895)


come



channel) protein

Not in xenbase

DLL4 (ASHY131923

)


come xdll4, delta

2, x-dll4



Not in xenbase

Etsrp (AOPZ27756)


come er71,

etsrp71, Etsrp Predicted

transcription facto

Not in xenbase

CD41 (AOPZ34290)


come

cd41, cd41b, gp2b, gpiib, gta, hpa3



xenbase

Cx37 (AOPZ19634)


comeconnexin 37,

cx37 connexon channel

component Not in

xenbase

1559 gfi1a

1560

1561

1562

1563

1564

1565

Gfi1 (AHSY129235

)



Not in xenbase

Fli1 (AHSY95605)




Not in xenbase

Fli1 (AHSY109769

)




Not in xenbase

Lmo2 (AHSY129032

)


come

LMO-2, xlmo2, xlmo-

2 transcription

factor Not in

xenbase

MMP9 (AHSY137493

)


come

MMP-9, gelb, clg4b,

mandp2

extracellular matrix

metalloprotease

Not in xenbase

Prominin1 (AOPZ5251)


come



cord12, proml1

polytopic membrane

protein Not in

xenbase

CEBPdelta (AHSY16156)


come


clone

Not in xenbase

1566

1567

1568

1569

1570

1571

1572

Tcf21 (AHSY20090)


come capsulin, pod

1, pod1

helix-loop-helix


Not in xenbase

CD93 (AHSY191408

)


come

c1qr1, c1qrp, cdw93, ecsm3, mxra4,

c1qr(p), c1qr, AA4



EGF-like domains

Not in xenbase

Pax2 (AHSY13273)


come XPax2, XPax-

2, pax-2paired box


Not in xenbase

Atp1a1 (AHSY14244)


come

Na+/K+ ATPase, Na+K+ ATPase,

Na+K+ATPase, K-ATPase

cation transporting

ATPase subunit

Not in xenbase

Evi1 (AHSY64565)


come


ecotropic viral

integration site 1


Not in xenbase

Lim1 (AHSY251630

)


come

Xlim1, Xlim-1, Lim1, lim-1,

lim1

LIM and homeodomain containing transcription

factor

Not in xenbase

Eto2 (AOPZ93198)


come

eto2, mtg16, mtgr2,

zmynd4, mtg16b


Not in xenbase

1573

1574

1575 CARP

1576 arpp

1577

1578

1579

ALK1 (AOPZ61326)


come

alk1, alk-1, hht2, orw2, skr3, tsr-i, acvrlk1

activin receptor,


kinase

Not in xenbase

Ankyrin1 (AHSY11469)


come


clone

Not in xenbase

Ankrd1 (AHSY24670)


comeAnkyrin repeat

Not in xenbase

Ankrd2 (AHSY158931

)


come

Myotrophin and similar

proteinsNot in

xenbase

Seb4R (AHSY170461

)


come rnpc1, XSeb4R

nucleic acid binding

Not in xenbase

Prox1 (AOPZ242825

)


come

Prox 1, prospero-related

homeobox 1, prospero,

xprox1

homeodomai

n transcription

factor

Not in xenbase

CD31 (AHSY76538)


come

cd31, alpha-globin, alpha

globin

hemoglobin, oxygen

transportNot in

xenbase

1580

1581

1582 n/a

1583

1585 FOG

1586 CD41

1587 Prom1

Ikaros (AOPZ125052

)


come

zinc finger protein,

subfamily 1A, 1, znfn1a1,

ik1, lyf1, ik-1, ikaros,

pro0758, znfn1a1, ika


Not in xenbase

Hex (AHSY108553

)


come XHex, tHex,

hex

homeodomain


Not in xenbase

Rasip1 (AOPZ116339

)


comeMyosin class

V heavy chainNot in

xenbase

DAB2 (AOPZ95258)


comexdab2, doc2,

doc-2adaptor protein

Not in xenbase


come



Not in xenbase


come

cd41, cd41b, gp2b, gpiib,

gta, hpa



xenbase


come



cord12, proml1

polytopic membrane

protein Not in

xenbase

1588 Rspo3 n/a

1589 KLF5

1590 Elk1

1591 Eos eos, znfn1a4

1592 CEBPz n/a

1593

1594


come

subtilisin-like proprotein

convertase; regulates the

balance between

angioblast and blood cell specification

Not in xenbase


come cklf, iklf,

bteb2 zinc finger, C2H2 type

Not in xenbase


come elk-1, Xelk-1,

XelkPredicted


Not in xenbase



Not in xenbase


come

ribosomal subunit

biogenesis protein

(transcription factor)

Not in xenbase

Smad6-WT-pGEMT


comemadh6, Xsmad6


TGF-beta pathway

Not in xenbase

Smad6-Mut-pGEMT


comemadh6, Xsmad6


TGF-beta pathway

Not in xenbase

1600 XPitx2

1601 Dtx1

1602 Dtx2 n/a

1603 ppp3ca

1604 cpped1 n/a

1608

1609


come

pitx2-A, xPtx2, xpitx2,

pitx2c

homeodomain


Not in xenbase


comeXdtx1, dtx,

deltexubiquitin

ligaseNot in

xenbase


come

Putative ubiquitin

fusion degradation

protein

Not in xenbase


come

caln, ccn1, cna1, calna,

ppp2b, calna1,

calcineurin A alpha,

calcineurin

Serine-threonine

phosphatase 2B, catalytic

subunit

Not in xenbase


come Purple acid phosphatase

Not in xenbase

pCS2+3FlagMAM1-GR


come

XMam1, Mastermind1, mam1, mam-

1

notch signal component

Not in xenbase

pCS2+3FlagdnMAM1-GR


come

XMam1, Mastermind1, mam1, mam-

1

notch signal component

Not in xenbase

1610 Vector

1611 dHAND

1612 Mef2d

1613 FGF8b

1614 TnIc Troponin I

1615 Hes2 XHes2

1616

pSP64T-GR-HA


comeNot in

xenbase


come

dhand, dhand2, xhand2



Not in xenbase


come XMEF2D, sl1,

sl-1 MADS-box


Not in xenbase


come

mgc79739, Xfgf-8, Xfgf8, FGF-8, fgf8a, fgf8b, fgf-8b

heparin-binding


xenbase


come

cardiac troponin I, TnIc, ctnIc,

cTNT, cardiac tropin T, XTnIc, c-troponin

Not in xenbase


come

helix-loop-helix


ptional repressor

Not in xenbase

Notch4 5' RACE


come


ligands

Not in xenbase

1618 ESR9b esr9

1619 Dll2

1620 Hey2

1627 RITA RITA n/a

1630

1631 Hes6

1632


come

helix-loop-helix


Not in xenbase


come X-dll2, tes1,

tes-1

homeodomai

n and LIM zinc binding

domain transcription

factor

Not in xenbase


comehesr2,

gridlock

helix-loop-helix


ptional repressor

Not in xenbase


comeNot in

xenbase

Notch4 5' RACE


come


ligands

Not in xenbase


comeXHes6, Xhes-

6

helix-loop-helix


ptional repressor

Not in xenbase

Notch4-like with ATG


come


ligands

Not in xenbase

1634

1635

1636 Sarcolipin

1637 Glypican-like

1638 Dll1

1639 CEBPalpha

1640 HIF1alpha

caALK1 (constitutivel

y active ALK1)


come

alk1, alk-1, hht2, orw2, skr3, tsr-i, acvrlk1

activin receptor,


kinase

Not in xenbase

caALK5 (constitutivel

y active ALK5)


come

aat5, alk5, skr4, alk-5,

lds1a, lds2a, tgfr-1, acvrlk4

serine/threonine

kinaseNot in

xenbase


come


clone

Not in xenbase


come


clone

Not in xenbase


come




Not in xenbase


come

C/EBPalpha, xC/EBP, C/EBP


factor

Not in xenbase


come

hif1alpha, hif-1alpha, xhif1a

helix-loop-helix


Not in xenbase

1641 CEBPbeta

1642 CEBPgamma cebpg

1643 Granulin

1644 Thbs2 tsp2


1646 Nurr1

1647 Irx4


come C/EBPbeta, xC/EBPbeta


factor

Not in xenbase


come


factor

Not in xenbase


come

granulin, gep, gp88, pepi, pgrn,

pcdgf, acrogranin, progranulin, proepithelin

Epithelin/gran

ulin Not in

xenbase


come

Immunoglobin and related

proteins

Not in xenbase


come transcription

factor Not in

xenbase


come

NGFI-B, rnr1, hzf-3, nurr1,

tinur hormone receptor

Not in xenbase


comeiroquois-4,

irxa3, Xiro4

homeodomai

n transcription

factor

Not in xenbase

1649 ID4

1650 Lmo2

1651 Osr1 odd1

1652 Osr2 xosr2

1653 SMP30

1654 PDZK xpdzk1 PDZ domain

1655 Ldb1


come idb4,

bhlhb27


Not in xenbase


come

LMO-2, xlmo2, xlmo-

2 transcription

factor Not in

xenbase



Not in xenbase



Not in xenbase


come

xsmp-30, smp30, xsmp30,

regucalcin

gluconolacton

ase Not in

xenbase


comeNot in

xenbase


come xldb1, nli,

clim2 transcription

cofactor Not in

xenbase

1656 Itga6 n/a

1657 Mtm1 n/a

1658 Gpr56 n/a

1659 VLDLR

1660 Acvr2a

1661 FoxO3

1662 ZFAT


come

cell surface receptor subunit

Not in xenbase


come

Phosphatidylinositol 3-

phosphate 3-phosphatase myotubularin

MTM1

Not in xenbase


come

G protein-coupled

receptorsNot in

xenbase


come vitellogenin

receptorEGF-like

calcium ion binding

Not in xenbase


comeacvr2, actrii,

XSTK9

transforming growth

factor-beta receptor

Not in xenbase


come foxo3a, xfoxo3

forkhead domain


Not in xenbase


comezfat1, ZFAT

zinc finger 1 zinc finger, C2H2 type

Not in xenbase

1663 FAM44B fam44b n/a

1664 NFATC3

1665 WASL

1666 USP6NL n/a

1667 Pard3 par3, par-3 PDZ domain

1668 CD51 itgav

1669 Scrib scrb1


comeNot in

xenbase


come nf-at, nfat4,

nfatx

immune response


Not in xenbase


comenwasp, n-

wasp actin

regulatory protein

Not in xenbase


come

Rab6 GTPase activator

GAPCenA and related TBC

domain proteins

Not in xenbase


comeNot in

xenbase


come

cell surface receptor subunit

Not in xenbase


come Leucine rich

repeat Not in

xenbase

1670 Hyrax

1671 Sfrp1

1672 ZCCHC14 n/a

1673 Evi5 n/a

1674 Scrib scrb1

1789


come

cdc73 [Predicted: Source is Human

Entrez Gene]

RNA polymerase II

assessory factor Cdc73p

Not in xenbase


come

frzA, frp, frp1, frp-1,

sarp2, XfrzA modulator of Wnt signaling

Not in xenbase


come

Predicted RNA-binding

protein involved in

translational regulation

Not in xenbase


come

Predicted GTPase

activator NB4S/EVI5

(contains TBC domain)/Calm

odulin-binding

protein Pollux (contains PTB

and TBC domains)

Not in xenbase


come Leucine rich

repeat Not in

xenbase

hesr1-pDonor221


come



helix-loop-helix


ptional repressor

Not in xenbase

1790 hesr1-GFP

1791

1792 hesr2-GFP

1793

1794 Notch1-GFP

1795 HoxC4 n/a

1796 HoxC9 Xhoxc9


come



helix-loop-helix


ptional repressor

and Fluorescent

Protein

Not in xenbase

hesr2-pDonor221


comehesr2,

gridlock

helix-loop-helix


ptional repressor

Not in xenbase


comehesr2,

gridlock

helix-loop-helix



Protein

Not in xenbase

Notch1-pDonor221


come



ligands

Not in xenbase


come


signal transduction, receptor for delta class ligands and Fluorescent

Protein

Not in xenbase


come

homeodomai

n transcription

factor

Not in xenbase


come

homeodomain


Not in xenbase

1797 HoxC3 n/a

1798 HoxC8

1799 HoxD9

1800 Esr10-GFP n/a

1801 Hes3a

1802 Hes3b

1803 Hes8 n/a


come

Transcription factor

zerknullt and related HOX

domain proteins

Not in xenbase


comexhoxc8, hoxc-

8

homeodomain


Not in xenbase


come

Xhoxd9, hox4c, hox-4.3, hox-5.2

homeodomai

n transcription

factor

Not in xenbase


come

n/a and Fluorescent

ProteinNot in

xenbase


come esr6e, esr-6e

helix-loop-helix


ptional repressor

Not in xenbase


come esr6e, esr-6e

helix-loop-helix


ptional repressor

Not in xenbase


come

transcriptiona

l repressor Not in

xenbase

1804 Hoxc3 n/a

1805 Hoxc8

1806 Hoxd9

1807 Hoxb5

1853

1854 Vector


come

Transcription factor

zerknullt and related HOX

domain proteins

Not in xenbase


comexhoxc8, hoxc-

8

homeodomain


Not in xenbase


come

Xhoxd9, hox4c, hox-4.3, hox-5.2

homeodomai

n transcription

factor

Not in xenbase


come

XlHbox4, Hox2.1, Xhox1B

homeodomain


Not in xenbase

EmTQ2 turquiose protein


comeFluorescent

ProteinNot in

xenbase

pDESTtol2pA2


comeNot in

xenbase

1855 Vector

LJ-65 ntpd2 ion channel

LJ-73

LJ-74

pDESTtol2CG2


comeNot in

xenbase

phosphodiesterase

phosphodiesterase

has no sequence-Maya Piccinni

A823

has no sequence -Maya Piccinni

LJ-76 ntpd1 ion channel

LJ-79 enpp2

LJ-80 ntpd2

LJ-87

LJ-88

LJ-89 enpp4

phosphodiesterase

phosphodiesterase

phosphodiesterase

phosphodiesterase

phosphodiesterase


LJ-90 vector

LJ-91 vector

LJ-92 vector

LJ-93

LJ-94

LJ-95

phosphodiesterase

phosphodiesterase

phosphodiesterase


LJ-96

LJ-97

LJ-98

LJ-99

LJ-100

LJ-101

LJ-102

phosphodiesterase

phosphodiesterase

phosphodiesterase

phosphodiesterase

phosphodiesterase

phosphodiesterase

phosphodiesterase


LJ-103

LJ-104

LJ-105

LJ-106

LJ-107

LJ-108

LJ-109

phosphodiesterase

phosphodiesterase

phosphodiesterase

phosphodiesterase

g-protein coupled receptor




LJ-110

LJ-111

LJ-116 ntpd1

LJ-117 not known

LJ-118 TF

LJ-119 not known



nucleotide phosphatase

not in Xenbase

not in Xenbase

not in Xenbase

not in Xenbase

not in Xenbase


LJ-120 TF

LJ-121 eye TF

LJ-122 eye TF

LJ-123 TF

LJ-124

LJ-125 RTK

LJ-126

not in Xenbase

not in Xenbase

not in Xenbase

not in Xenbase


not in Xenbase

not in Xenbase

transmembrane solute exchange

not in Xenbase


LJ-127

LJ-128 TF

LJ-129

LJ-130 not known

LJ-131 not known

LJ-132

LJ-133 not known

TGFb signal modulator

not in Xenbase

not in Xenbase

chloride channel

not in Xenbase

not in Xenbase

not in Xenbase

transmembrane cell

signallingnot in

Xenbase

not in Xenbase


LJ-134

LJ-135

LJ-136 TF

LJ-137 not known

LJ-138 not known

LJ-139

LJ-140

BMP receptor?

not in Xenbase

Activin recptor?

not in Xenbase

not in Xenbase

not in Xenbase

not in Xenbase

signalling molecule

not in Xenbase

TGFb modulator

not in Xenbase


LJ-141

LJ-142

LJ-143

LJ-144

LJ-145

LJ-146

LJ-147

g-protein coupled recptor

not in Xenbase


not in Xenbase


not in Xenbase


not in Xenbase


not in Xenbase


not in Xenbase


not in Xenbase


LJ-148

LJ-149

LJ-150

LJ-151

LJ-152

LJ-153

LJ-154 cyclin


not in Xenbase


not in Xenbase


not in Xenbase

cyclin dependent

kinasenot in

Xenbase

cyclin dependent

kinase inhibitor

not in Xenbase

cyclin dependent

kinase inhibitor

not in Xenbase

not in Xenbase


LJ-155

LJ-156 cytochrome

LJ-157

LJ-158

LJ-159 ser/thr kinase

LJ-160

LJ-161 peroxidase

cyclin dependent

kinase inhibitor

not in Xenbase

not in Xenbase


not in Xenbase


not in Xenbase

not in Xenbase

signalling molecule

not in Xenbase

not in Xenbase


LJ-162

LJ-163

LJ-164

LJ-165

LJ-166

LJ-167

LJ-168

ribosomal protein

not in Xenbase

ribosomal protein

not in Xenbase


not in Xenbase

kinase regulator

not in Xenbase

kinase regulator

not in Xenbase

ubiquitin ligase

not in Xenbase

mesoderm transcription

factornot in

Xenbase


LJ-169

LJ-170 not known

LJ-171

LJ-172 cytoskeleton

LJ-173 not known

LJ-174

LJ-175

mesoderm transcription

factornot in

Xenbase

not in Xenbase

phospholipid binding

not in Xenbase

not in Xenbase

not in Xenbase

neural crest TF

not in Xenbase

DNA synthesis inhibitor

not in Xenbase


LJ-176 ser/thr kinase

LJ-177

LJ-178 not known

LJ-179 not known

LJ-180

LJ-181

LJ-182

not in Xenbase

phospholipid binding

not in Xenbase

not in Xenbase

not in Xenbase

notch modulator

not in Xenbase

notch modulator

not in Xenbase

myc [Predicted: Source is Human

Entrez Gene]

v-myc avian myelocytoma

tosis viral oncogene homolog

[Predicted: Source is Human

Entrez Gene]


cmyc transcription

factor not in

Xenbase


LJ-183 tcf3

LJ-184

LJ-185 met c-Met

LJ-186

LJ-187

LJ-188

LJ-189 lhx1




not in Xenbase

fluorescent protein

not in Xenbase

met proto-oncogene

tyrosine kinase, HGF

receptor not in

Xenbase

not in Xenbase

not in Xenbase

not in Xenbase

LIM homeobox 1

Xlim1, Xlim-1, Lim1, lim-

1, lim1


factor

not in Xenbase


LJ-190 lhx1

LJ-191

LJ-192 tcf12 HEB

LJ-193 tcf12 HEB

LJ-194 n/a enzyme

LJ-195 wt1 xwt1, wt-1

LJ-196

LIM homeobox 1

Xlim1, Xlim-1, Lim1, lim-

1, lim1


factor

not in Xenbase

not in Xenbase


(HTF4, helix-loop-helix


helix-loop-helix


not in Xenbase


(HTF4, helix-loop-helix


helix-loop-helix


not in Xenbase

glb1 [Predicted: Source is Human

Entrez Gene]



Entrez Gene]

not in Xenbase

Wilms tumor 1

zinc finger transcription and splicing

factor

not in Xenbase

not in Xenbase


LJ-197

LJ-198

LJ-199

LJ-200 nphs1 nephrin

LJ-201 slc12a1

LJ-202 slc12a1

LJ-203

not in Xenbase

not in Xenbase

not in Xenbase

nephrosis 1, congenital, Finnish type (nephrin)

transmembra

ne protein, component of

slit diaphragm,

cell signaling

not in Xenbase


(sodium/potassium/chlorid

e transporter), member 1

XNKCC2, Na-K-2Cl

cotransporter, NKCC2, NKCC-2

transmembra

ne solute exchange

not in Xenbase


(sodium/potassium/chlorid

e transporter), member 1

XNKCC2, Na-K-2Cl

cotransporter, NKCC2, NKCC-2

transmembra

ne solute exchange

not in Xenbase

not in Xenbase


LJ-204

LJ-205

LJ-206

LJ-207

LJ-208

LJ-209

LJ-210 cndp2

not in Xenbase

not in Xenbase

not in Xenbase

not in Xenbase

not in Xenbase

not in Xenbase

CNDP dipeptidase 2 (metallopepti

dase M20 family)

cn2, cpgl, darmin-r

metallopeptidase

not in Xenbase


HW - 1 TF

HW - 2 TF

HW - 3 Tf

HW - 4 TF

HW - 5 TF

HW - 6 TF

HW - 7 TF


HW - 8 TF

HW - 9 TF

HW - 10 TF

HW - 92 TF

HW - 93 TF

HW - 94 TF

HW - 95 TF


HW - 96 TF

HW - 97 TF

HW - 98 TF

HW - 99 TF

HW - 100 TF

HW-11

HW-12

germ plasm formation


HW-13

HW-21 TF

HW-22 TF

HW-25

HW-28

HW-29

HW-30


germplasm formation

germplasm formation

germplasm formation

germplasm formation

HW-32

HW-33

HW-37

HW-38

HW-39

SM-2

SM-4 TF



ventral pattern

ventral pattern

fluorescent protein

signal transduction

SM-5 TF

SM-6 TF

SM-9 TF

JG-1 ser/thr kinase

JG-2 ser/thr kinase

JG-3 ser/thr kinase

JG-4 ser/thr kinase

xkp78 / XMARK3/XPA

R-1A wild type

XMARK2/XPAR-1B wild

type

eGFP+ XMARK3/Xpar

-1A

JG-5 ser/thr kinase

JG-6 ser/thr kinase

48-ER

49-ER

50-ER

51-ER

52-ER DNA repair

xkp78 / XMARK3/XPAR-1A kinase

mutant

retinoic acid synthesis

aldehyde dehydrogena

se

cytochrome p450

53-ER

54-ER not known

55-ER

56-ER

57-ER RTK

58-ER not known

C136 cyclin

signalling molecule

signalling molecule

C145

C146

C149

C166 N/A

TH-28

TH-29

TH-30

cyclin dependent

kinase (CDK)

cdk3 gene not in

xenbase

cyclin dependent

kinase (CDK)

TH-31

TH-32

TH-33

TH-34

TH-35 ccnb2 cyclin B2

TH-36 cdk7

TH-37 n/a

C-B2, cyclin B2, cyclinb2

cell cycle regulator


p40MO15, MO15, stk1

Cdk activating

kinase not in

xenbase

rbx1 [Predicted: Source is Human

Entrez Gene]

ring-box 1, E3 ubiquitin

protein ligase [Predicted: Source is Human

Entrez Gene]

SCF ubiquitin ligase, Rbx1 component

not in xenbase

TH-38 n/a

TH-39

TH-40

TH-41 ccnc cyclin C n/a

TH-42 ccnh cyclin H n/a

TH-43

TH-44

rbx1 [Predicted: Source is Human

Entrez Gene]

ring-box 1, E3 ubiquitin

protein ligase [Predicted: Source is Human

Entrez Gene]

SCF ubiquitin ligase, Rbx1 component

not in xenbase

mad2l1 [Predicted: Source is Human

Entrez Gene]

MAD2 mitotic arrest

deficient-like 1 [Predicted:

Source is Human

Entrez Gene]

mad2, XMad2

Spindle assembly

checkpoint protein

not in xenbase

not in xenbase


not in xenbase


not in xenbase

not in xenbase

not in xenbase

TH-45

TH-46 eg1

TH-47

TH-48 ccnc cyclin C n/a

TH-49

TH-50

TH-51

not in xenbase

cdk2 [Predicted: Source is Human

Entrez Gene]



Entrez Gene]

Protein kinase

PCTAIRE and related kinases

not in xenbase

not in xenbase


not in xenbase

not in xenbase

not in xenbase

not in xenbase

TH-52

TH-1 cdk1

C5 cyclin

C8

TH-5 cdk17

C22 N/A

C23 kinase

not in xenbase


p34cdc2, cell division control

protein 2 homolog, cdc-

2, xcdc2, cdc2, cdc28a,

PSTAIR, cdc2x1.1

cyclin dependent

kinase (CDK)not in

xenbase


PCTAIRE2, pctk2

serine/threonine

kinase not in

xenbase

not in xenbase

C27 eg1

C29

TH-10

TH-11

C52 cdk1

C57 eg1

C60 kinase


Entrez Gene]



Entrez Gene]

cyclin dependent

kinase (CDK)XB-CLONE-12442548

cyclin dependent

kinase (CDK)

myc [Predicted: Source is Human

Entrez Gene]

v-myc myelocytoma

tosis viral oncogene homolog


Entrez Gene]


cmyc

cyclin dependent


cyclin dependent

kinase (CDK)





PSTAIR

cyclin dependent



Entrez Gene]



Entrez Gene]

cyclin dependent


not in xenbase

C80 cdk7 kinase

C82 ccnb2 cyclin B2 cyclin

C99 eg1

C108

C109

C124 cdk5 n/a

C127


p40MO15, MO15, stk1

XB-CLONE-12442567

C-B2, cyclin B2, cyclinb2

XB-CLONE-12442573


Entrez Gene]



Entrez Gene]

cyclin dependent


tumour supressor

tumour supressor


cyclin dependent


cyclin dependent

kinase (CDK)not in

xenbase

C134 xcdk4

5-KL wnt3

6-KL

7-KL

8-KL

9-KL TF

10-KL TF


Entrez Gene]



Entrez Gene]

cyclin dependent


wingless-type MMTV

integration site family, member 3

wnt3l, wnt-3, Xwnt-3, Xwnt3

signalling molecule

signalling molecule

signalling molecule

signalling molecule

477 pat germ plasm

538 dHand

581 Flk-1

659 Uncx4-1

HAR-152

HAR-153 not known

HAR-154 TF

XB-CLONE-12441203


come

dhand, dhand2, xhand2



Not in xenbase


come


vegfr2, xflk-1


Not in xenbase


come uncx4.1, uncx-4.1

homeodomai

n transcription

factor

Not in xenbase


HAR-164 foxh1 TF

HAR-161 TF

HAR-180 RTK

HAR-186 ephb3 tck RTK

HAR-197 tll2 tolloid-like 2 xolloid, Xld

HAR-200 TF

HAR-461 No DNA Not available

forkhead box H1

fast1, fast-1, XFast-1, xfoxh1, xfast1, foxh1a

XB-CLONE-4048730

EPH receptor B3

XB-CLONE-4125616

metalloprotease

XB-CLONE-4227561


HAR-203 TF

HAR-206 TF

HAR-229

HAR-210 TF

HAR-219 TF

HAR-225 TF

HAR-226 cell adhesion

HAR-239 TF

HAR-240

HAR-247

HAR-254 TF

HAR-257 heart/gut TF

HAR-260

vegetal formation

metabolic enzyme

signalling molecule

HAR-266

HAR-263

HAR-264 heart/gut TF

HAR-279 Not available

HAR-304 No DNA

HAR-347 No DNA

HAR-348 No DNA

signalling molecule

solute transport

signalling molecule


HAR-271

HAR-274 TF

HAR-276 TF

HAR-292 cytoskeletal

HAR-298 RTK

HAR-318 TF

structural protein

HAR-360 Not available


HAR-363 smarca1 snf2l



SWI/SNF related, matrix

associated, actin

dependent regulator of chromatin,

subfamily a, member 1

chromatin remodeling

complex component

HAR-365 n/a

HAR-368 chd4 n/a

HAR-369 n/a

HAR-370 chd9 n/a



chd1 [Predicted: Source is Human

Entrez Gene]

chromodomain helicase

DNA binding protein 1


Entrez Gene]

Chromodomain-helicase

DNA-binding protein



Predicted helicase

chd6 [Predicted: Source is Human

Entrez Gene]




Entrez Gene]

Chromodomain-helicase

DNA-binding protein



chromatin assembly/dis

assembly

HAR-373 vsx2

HAR-374 tbr1

HAR-375 six1 XSix1

HAR-376

HAR-377 six3 XSix3, hpe2

HAR-378 foxg1

HAR-379 dlx2

visual system homeobox 2

chx10, xchx10

homeodomain


T-box, brain, 1

xtbr1, tbr-1, T-brain-1

T-box transcription

factor

SIX homeobox 1

homeodomain


SIX homeobox 3

homeodomain


forkhead box G1

XBF-1, foxg1b, bf1,

XBf1

forkhead domain



X-dll2, tes1, tes-1

homeodomain and LIM

zinc binding domain


HAR-380 Distal-less 4

HAR-384 nkx2-1

HAR-385 ptn pleiotrophin growth factor

HAR-386 XEmx1

HAR-387 emx2



dlx4 [provisional]


homeodomain and LIM

zinc binding domain


NK2 homeobox 1

ttf1, nkx2.1, titf1, Xnkx-

2.1, xnkx2-1

homeodomain


X-PTF, X-ptf, pleiotrophin

emx1 [provisional]

empty spiracles


homeodomain


empty spiracles

homeobox 2xemx2, emx-

2

homeodomain





HAR-441

HAR-449 hmx3

HAR-466 nkx2-2

HAR-438

H6 family homeobox 3

nkx5.1, nkx5-1, nkx-5.1

homeodomain


XB-CLONE-3283669

XeNK-2, nkx2.2, Xnkx2.2

homeodomain


HAR-439

9-SPH gsc-a TF

38-SPH

42-SPH

552-SPH pygo2-b Xpygo2

176-SPH gsk3a xgsk3

43-NP RTK

goosecoid homeobox

Xgsc, goosecoid

XB-CLONE-14573950

pygopus homolog 2


Entrez Gene]


XB-CLONE-14574231


not in xenbase

can not associate it with a gene

not in xenbase

NP-C adnp n/a

23-ER TF

17-ER TF

868 ripe3b1

885 Catalase

886 Vito-b

activity-dependent

neuroprotector homeobox

homeodomai

n transcription

factor

XB-CLONE-4072319

not in xenbase

mafa [provisional]

v-maf musculoapon

eurotic fibrosarcoma

oncogene homolog A

[provisional]

leucine zipper transcription



comeNot in

xenbase


comeNot in

xenbase

888 Robo4

898 Pgk1

899 MCL1

900

901 CHOA

904 hipk2 n/a

913 XHR1


comeNot in

xenbase


comeNot in

xenbase


comeNot in

xenbase

Na-Cl cotransporter


comeNot in

xenbase


comeNot in

xenbase

homeodomain interacting

protein kinase 2

dual-specificity tyrosine-

phosphorylation regulated

kinase

XB-CLONE-2160489


comeNot in

xenbase

914 nrp2 n/a

924 Int7

925 Etv2

927 nab2 mader

929 Snrk n/a



transmembrane receptor

XB-CLONE-2197914


comeNot in

xenbase


comeNot in

xenbase

NGFI-A binding

protein 2 (EGR1 binding

protein 2) [Predicted: Source is Human

Entrez Gene]

Transcriptional

corepressor NAB1


come

Serine/threonine protein kinase

Not in xenbase



XB-CLONE-11536572



XB-CLONE-11536575

LJ-18 not available not known

LJ-19 not available TF

LJ-21

LJ-44

LJ-47

not in xenbase

not in xenbase

signalling molecule

not in xenbase

signalling molecule

not in xenbase

signalling molecule

LJ-48

LJ-50

CS-241

CS-396 cytoskeleton

3-KL TF

4-KL pax2 paired box 2 TF

15-KL TF

signalling molecule

signalling molecule

retinoid receptor

not in xenbase

not in xenbase

XPax2, XPax-2, pax-2

XB-CLONE-14574882

21-KL not known

24-KL vector

53-KL luciferase

54-KL

69-KL RNA binding

87-KL

101-KL


vegt [provisional]


brat, xombi, antipodean, tVegT, Apod

T-box transcription factor, meso-endodermal determinant

XB-CLONE-12442485

transcription coactivator

147-KL

160-KL

161-KL

169-KL

170-KL

AP-134 kinase

136-AP ccne2 cyclin E2 n/a cyclin

could not assiciate it with gene


not in xenbase

flurescent protein

not in xenbase

wnt signalling/cell

adhesion



not in xenbase



not in xenbase

XB-CLONE-12271894

AP-135 ccne, cyclinE

138-AP cdk1

140-AP rb1

234-AP atoh7 TF

415-AP tp53

1101-AP isl1 TF

ccne1 [Predicted: Source is Human

Entrez Gene]

cyclin E1 [Predicted: Source is Human

Entrez Gene]

G1/S-specific cyclin E

XB-CLONE-12271893





PSTAIR

cyclin activator

XB-CLONE-12271897

retinoblastoma 1

XRb1, retinoblastom

a proteintumour

supressorXB-CLONE-12271900

atonal homolog 7

Xath5b, math5, ath5,

Xath5XB-CLONE-12440832

tumor protein p53

p53, Xp53, Li-Fraumeni syndrome


regulating cell cycle,

apoptosis and TGF-beta signaling

XB-CLONE-12440929

ISL LIM homeobox 1

islet-1, Xisl-1, Xislet-1,

islet1XB-CLONE-12442053


Maya Piccinni:is on xenbase but has no sequence. Sequence held is not good has to be re-sequenced

Maya Piccinni:not in xenbase, no dna no info

Maya Piccinni:no good sequence, sequence not in xenbase


DXAR DXAR not needed


Xmsr pIBI Xmsr

???? ????

ODC2

Xnr2

LFABP 5'

pSPxSox17alpha

TG12 Δ 10

TG10.5 Δ 7.5

TG10 Δ 5

TG11 + 1.7

TG10.5 Δ 7.5

TG10.5 Δ 7.5-5

pBS TG12 Δ 10-5

pSD VegT VP16

TG8.5 + C3

TG0.5 + 1.7

pGL3 ProxPr

GL3 B1

GL3 C3/B1

GL3 C3

TG8.5 + C3/B1

MR19

MR21

pMC-Hermes

pMC-Xvelo FL

pHermes-Yfp (1HY)

pYFP Hermes 2YH

peGFP-Xvelo FL

PMC-Xvelo SV

pCER-Xvelo FL

peGFP-Xvelo SV

pCER-Xvelo SV

peGFP-Rbm24b

pCER-Rbm24b

pCER-Rbm42b

peGFP-Xvelo FL Δ1

peGFP-Xvelo FL Δ2

peGFP-Xvelo FL Δ3

pCER-Xvelo FL Δ1

pCER-Xvelo FL Δ2

pCER-Xvelo FL Δ3

pMC-Xvelo FL Δ3

peGFP-Xvelo SV Δ1

pCER-Xvelo SV Δ1

pVN154m9-Xvelo FL

pVN154m9-Xvelo SV

pVN154m9-Hermes

pVN154m9-Rbm24b

pVN154m9-Rbm42b

pVC155-Pix1

pVN154m9-Pix1

pVC155-Xvelo FL

pVC155-Xvelo SV

pVC155-Hermes

pVC155-Rbm24b

pVC155-Rbm42b

BMP4

Nkx2-4

bmp-4 bmp4 bone

nkx-2.4 nkx2.4 nkx2-

4

Vector sequence with sp6

XB-CLONE-11536601

ntpd2 pBF ntpd2 pBF not needed

enpp4 DipGn pCS2+

enpp4 DipGn pCS2+

enpp2 pCDNA3

enpp2 pCDNA3


ntpd1 pBF ntpd1 pBF not needed

enpp2b pBF enpp2b pBF not needed

not needed

not needed

Mouse entpd2 pBF

Mouse entpd2 pBF

enpp4 T72S pCS2+

enpp4 T72S pCS2+

enpp4 T72A pCS27

enpp4 T72A pCS27

enpp4 pBSRN3

enpp4 pBSRN3


In situ Clones

P2Y1 pBSKS1 P2Y1 pBSKS1

P2Y1 pBSKS2 P2Y1 pBSKS2

P2Y8 pBSKS P2Y8 pBSKS

entpd1 entpd1

entpd2 entpd2

entpd3 entpd3


entpd4 entpd4

entpd5 entpd5

entpd6 entpd6

entpd7 entpd7

enpp1 enpp1

enpp2a pBF enpp2a pBF

enpp2b pBF enpp2b pBF


enpp3 enpp3

enpp4 enpp4

enpp6 enpp6

enpp7 enpp7

LPA1.1 LPA1.1

LPA2 LPA2

LPA3 LPA3


LPA4 LPA4

LPA5 LPA5

entpd1 entpd1

ITSI-3 ITSI-3

pCS2 R Pax6 pCS2 R Pax6

XET XET

no name in box


Rx PGM3 Rx PGM3

pBS optx2 pBS optx2

sox3 pGEMT sox3 pGEMT

cres pGEMT cres pGEMT

xRet xRet

nkcc2 nkcc2

X.Six3 (Guido)

X.Six3 (Guido)


nog1 nog1

Pax8 Pax8

clck clck

xSevase xSevase

Nephrin Nephrin

xf25 3'-1 4 TOPO

55260504

xf25 3'-1 4 TOPO

55260504

pCS2+ xf37 (s/b)

pCS2+ xf37 (s/b)


BR BR

xAR1 xAR1

xFD xFD

dnty dnty

efrf efrf

BMP45 BMP45

noggin noggin


LPA4 IMAGE LPA4 IMAGE

LPA2 IMAGE LPA2 IMAGE

LPA1.1 IMAGE LPA1.1 IMAGE

xLPA3 IMAGE xLPA3 IMAGE

S1P4 IMAGE S1P4 IMAGE

S1P1 IMAGE S1P1 IMAGE

S1P3 Clone 2 pBS KS-

S1P3 Clone 2 pBS KS-


S1P5 pBS KS- S1P5 pBS KS-

S1P4R IMAGE S1P4R IMAGE

Lpar5/pCMV-Sport6

Lpar5/pCMV-Sport6

cdc2 pCS2+ QP2

cdc2 pCS2+ QP2

p27 xic-pCS2+ QP2

p27 xic-pCS2+ QP2

p27 xic1 N-pCS2+ QP1

p27 xic1 N-pCS2+ QP1

xCyclin A2 pCS2+ QP2

xCyclin A2 pCS2+ QP2


ID2 CS1oz A ID2 CS1oz A

ID4a ID4a

Par-1 BX Par-1 BX

p27 xic1 2 pCS2+ QP1

p27 xic1 2 pCS2+ QP1

LP21 Cy1 pCS2+ QP1

LP21 Cy1 pCS2+ QP1

VEGF pEAS4 forward 1

VEGF pEAS4 forward 1

xperox pu/po pGEMT

xperox pu/po pGEMT


LP14 pGEMTe LP14 pGEMTe

LP2 pGEMT LP2 pGEMT

SIP2 pBSKS SIP2 pBSKS

SIP1 pBSKS SIP1 pBSKS

E2 + SK E2 + SK

pxbra3 64T pxbra3 64T

LPA 1.1 pBSKS

LPA 1.1 pBSKS


xEX pCS4 xEX pCS4

anx4 pGEMT anx4 pGEMT

an4 trop an4 trop

CS2 geminin CS2 geminin

pxbra pBSKII+

pxbra pBSKII+

B4a-tubulin/pBS-KS

B4a-tubulin/pBS-KS

slug PCR pGEMT

slug PCR pGEMT


talpid talpid

pclta sport6 pclta sport6

senuk pBS senuk pBS

xanx4 pcs2+MT

xanx4 pcs2+MT

red Fng Mp Sport6

red Fng Mp Sport6

Lum Fng Mp Sport6

Lum Fng Mp Sport6

Myc-PODI pCS3+

Myc-PODI pCS3+


GFP:JC2L GFP:JC2L

XC - Met (2b) XC - Met (2b)

XG DNF (3b) XG DNF (3b)

XG DNF (4b) XG DNF (4b)

E12 pSP64 for mRNA

E12 pSP64 for mRNA

XC - Met pBSK (1b)

XC - Met pBSK (1b)

xLim1 for mRNA pSP64

xLim1 for mRNA pSP64


XonH1 pBSKS XonH1 pBSKS

HEB pCS2T HEB pCS2T

Bgal xnb SP6 Bgal xnb SP6

Xwt pGEM7 Xwt pGEM7

xLim1 for In Situ pBSKS

xLim1 for In Situ pBSKS

HEB pBS for In Situ

HEB pBS for In Situ

Clck for in situ

Clck for in situ


xCres pEasy xCres pEasy

NKCC-2 NKCC-2

No Glucose for In situ

No Glucose for In situ

Pux8 for In Situ

Pux8 for In Situ

Nephrin n2 for In Situ

Nephrin n2 for In Situ

NKCC-2 in situ

NKCC-2 in situ

TF4 pBS for in situ

TF4 pBS for in situ


xlob1 p54R1 xlob1 p54R1

POOL IMAGE POOL IMAGE

Cardiac Tropomyosin

pGEMT

Cardiac Tropomyosin

pGEMT

xlmx mut pRN3

xlmx mut pRN3

xlmx15 pRNλ3

xlmx15 pRNλ3

hE47 GENETIC

hE47 GENETIC

DarminR IMAGE

DarminR IMAGE


pBS Xsox17α pBS Xsox17α

pSP Xsox17α pSP Xsox17α

pSP Xsox17α +3’

pSP Xsox17α +3’

pSP Xsox17α :EnR

pSP Xsox17α :EnR

pSP Xsox17α HMG

pSP Xsox17α HMG

pSP Xsox17α TA

pSP Xsox17α TA

pSP Xsox17α MT

pSP Xsox17α MT


pSP Xsox17α VP16

pSP Xsox17α VP16

pSP Xsox17α GR

pSP Xsox17α GR

pCS107 xSox17α2

pCS107 xSox17α2

pBS Xsox17β pBS Xsox17β

pSP Xsox17β pSP Xsox17β

pSP Xsox17β +3’

pSP Xsox17β +3’

pSP Xsox17β :EnR

pSP Xsox17β :EnR


GST-xpat GST-xpat

pET21 xpat pET21 xpat

pSP Xsox17β HMG

pSP Xsox17β HMG

pSP Xsox17β TA

pSP Xsox17β TA

pSP Xsox17β MT

pSP Xsox17β MT

pSP Xsox17β MT2

pSP Xsox17β MT2

pSP Xsox17β VP16

pSP Xsox17β VP16

pET14 xpat pET14 xpat

pSP64TVP16 pSP64TVP16

pCS2+VegT pCS2+VegT

pET14bXpat pET14bXpat

pET21bXpat pET21bXpat

pJC2Xpat pJC2Xpat

pBS Xpat Full Length

pBS Xpat Full Length

pBS IκB pBS IκB

pSP64T IκB pSP64T IκB

peGFP-Ftase peGFP-Ftase

pBS-Six1 pBS-Six1

pET14b-XveloFL∆1

pET14b-XveloFL∆1

pET14b-XveloSV∆1

pET14b-XveloSV∆1

pBS-flotillin 1A

pBS-flotillin 1A

pDH105-six1 pDH105-six1

XKp78

pCS2+MT-six1

pCS2+MT-six1

pCS2+EnR-FoxD5

pCS2+EnR-FoxD5

pCS4+MT XKp78/XMARK3/XPAR-1A

wild type

XMARK2/XPAR-1B

pCS2+ + 5'UTR

MARK2/XPAR-1BX wild type

eGFP+ XMARK3/Xpar

-1A

pXt7/eGFP+MARK3

/XPAR-1A

eGFP+ XMARK3/Xpar

-1A T560A aPKC

phosphorylation site mutant

eGFP+ XMARK3/Xpar

-1A T560A aPKC

phosphorylation site mutant

xRDH10 xRDH10

xRALDH2 xRALDH2

xCYP26 xCYP26

Tex15

pCS2+myc PAR-1A

T560A aPKC phosphorelati

on site mutant

pCS2+myc PAR-1A

T560A aPKC phosphorelati

on site mutant

pCS4+MT XKp78/XMARK3/XPAR-1A

KM

Tex15/pBS-SK-

xGDNF

xc-Met

GDNF

tRET tRET/pCS2+

xc-met

cycD1 cycD1

xGDNF/pCMV-SPORT6

xc-Met/pBS SK-

GDNF ORF/pCS2+

xc-metTM/pCS2+

cdk3 cdk3

GST-cdk3 GST-cdk3

Protein G Protein G

Esp7

Esp11

Esp11 pCMx

Esp1

Esp2

C11

(C147) cycB2 cycB2

(C79) M015 GST MO15

Gst-xl.rb

LT10-Esp11x1 FZR

(C108) Gst-xl.rb

(C109) x1.rb x1.rb

KS46-CyclinH

Esp11A2 223

Esp11 224

LT12-pET21b x1 Mad2

LT5-pBSKS x1 FLR

KS35 GST-CyclinC

Esp3

Ky32 cyclinC

B1 not1

pGEM cdf1 myc TD

TP pGEM cdk2 myc

EST rbcl H6 pGEX KCRbx

1-H6

pGadge x1 B1 like

pET21b/xl B3

cdc2 (c1) cdc2

cycb2 cycb2

Protein A Protein A

PCTAIRE1 PCTAIRE1

cdc2.R2 91312-1314)

(c19)cdc2.R2

91312-1314)

cdk2-Globin cdk2-Globin not needed p no sequence

cdk5 cdk5

cdc2-myc not needed p no sequence

cdk2-myc not needed no sequence

GST-cdc2 GST-cdc2 not needed p no sequence

cdk2 cdk2 no pictures p no sequence

? no sequence

cdc2-myc (c44)

cdk2-myc (c49)

GST-PCTAIRE1-

myc

GST-PCTAIRE1-

myc

MO15 MO15 no pictures p no sequence

GST-cycA2 GST-cycA2 not needed p no sequence

GST-cdk2 GST-cdk2 not needed p no sequence

GST-XL-.RB GST-XL-.RB

XI.Rb XI.Rb


cdk6 cdk6


wnt3 cs7 wnt3

wnt3a wnt3a

wnt5a wnt5a

wnt7a wnt7a

ngn2 ngn2

pGlia3a pGli3a

pBS Xpat no pictures p no sequence

Crescent

Protein

En-2

crescent frzb2 frzb-2

engrailed-2 en2

Fast1 TEgg015n21 stage 24

Foxi3

Xelk eph receptor

ephb3 TGas045p01

Xolloid TNeu125j24 n/a p p

Msx1

Kl f -2

msx1 msx-1 xhox7.1

klf2 klf-2 kruppel-like

Erg

Msx2

Msx1

Nkx2-4

tfap2a ap2 ap-2

Endodermin endodermin

Xiro3

erg, vascular ets factor,

morphogenetic

msx1 msx-1 xhox7.1

xiro3 iro3 xiro-3 iro-3

ncam1

Sox17-alpha

Xpat Xpat

DG42

emx1 emx-1 emx1

Gata6 gata-6 gata6

Noggin2

NCAM neural cell

sox17alpha sox17-alpha

Hyaluronan synthase 1

noggin-2 noggin2

AL792266 CS107

kitlg

Vito vito

Gata6 gata-6 gata6

ADMP1 ADMP CS107

kit ligand, steel-2 stem

kr t10

Sox9 sox-9 sox9

Xcad1

Tanabin

kdr

En-1

cytokeratin epidermal

xcad1 cad1 cad-1

tanabin nestin

vegfr2 vegfr-2 flk1

engrailed1 engrailed-1

Smarca4 xtsmarca4

Smarca1 xtsmarca1

Chd1 xtChd1

Chd4 xtChd4

Chd6 xtChd6

Chd9 xtChd9

Dlx6

Rpx.1 Xanf

Sequenced as tropicalis cold inducible RNA

binding protein

(cirbp), mRNA

sequenced as reep3

Chx10

tbr1

six1

rx

six3

Bf1 Foxg1b

Dlx2 (XDLL4)

can not find gene-Maya Piccinni

G1126

can not find gene -Maya Piccinni

Dlx4

Nkx2.1 TTF1 titf1

PTF

Emx-1

Emx2

HoxB9 hoxb9


have to add as synonim to gene page-Maya Piccinni

G1132

have to add as synonim to gene page -Maya Piccinni

miR-181a2

Hmx3 no pictures p

Nkx 2.2

miR-124 no sequence

actinin, alpha4

alpha-4 actinin

microRNA miR-181

IMAGE:7674525

microRNA miR-124



miR-130a no sequence

p gsc2 p gsc2 no pictures no sequence

not needed p no sequence

xG114 not needed p no sequence

XFD II XFD II no pictures no sequence

microRNA miR-130


bug is resolved

pCS2+ Xpygo2beta

NHD

pCS2plus Xpygo2beta

NHD

XG114 (xGSK3)



ADNP TEgg085d20 no pictures no sequence

xGoosecoid xGoosecoid

Endodermin no pictures no sequence

MafA no pictures p no sequence

pBS Xenopus Endodermin

IMAGE:4201740



HIPK2 p pIMAGE:6643438

not in xenbase



Nrp2 no pictures p p

NAB2

no pictures p no sequence

Pod 1 pCS2+ no pictures p no sequence

Nrp2 (5572834)

NAB2 (7643352)

Pod 1 I.M.A.G.E CF270487

POD-1 IMAGE CF270487

Pod 1 pCS2plus



wnt6 wnt6 not needed

xtwnt9b xtwnt9b not needed

TXPom2 PBS 3

TXPom " PBS 3

XBapX I.M.A.G.E

XBapX I.M.A.G.E

XWnt 11 pBSKS

XWnt 11 pBSKS



wnt11 wnt11 not needed

wnt11-R wnt11-R not needed

RARa2-2 RARa2-2

XIF3 XIF3 no good read

BF1 BF1/pBSK

Xpax-b pBSXpax-b no pictures p

Pax-6 GFP Pax-6 GFP

no sequence aligment with

T7F



NLSMT1073S NLSMT1073S

GFP3STOP

pGL3 pGL3

pNG152 nrp1 RNP

Xt-box/CS105 Xt-box-CS105 not needed p no sequence

mPRP cs107-mPRP

cs107-GFP3STOP

CS4HA WTDvl-CAAX

CS4HA WTDvl-CAAX



XdshMA XdshMA

eGFP B/R not needed p

XdshD4 XdshD4

XdshD6 XdshD6

Xcdk4 Xcdk4

Xcyclin E2 Xcyclin E2 ? no sequence

CS2+ eGFP B/R

Csbcatenin ESDC

Csbcatenin ESDC



Xcyclin E Xcyclin E ? p no sequence

Xcdc2 Xenopus cdc2 ? p no sequence

XRb XRb pBSK no pictures p no sequence

Xath5 not needed p no sequence

Xp53 no pictures p p

IsletI pBSK2 IsletI no pictures p p

Xath5-pCS2plus

Xenopus p53-wt



Paper Vector Antibiotics Special Features

Expression pattern

not good with sp6

p p pBSCRN3

pIBI Amp

pBK-CMU KANAMYCIN

pSPJC2L Amp

pSP64T Amp

pcr TOP011 Amp

Hudson et al (1997) Cell 91, 397-405

pGL3

Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-415

pGL3

pGL3

pGL3

pGL3

pGL3

pGL3

pGL3

Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-416Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-417Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-418Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-419Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-420Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-421Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-422

pGL3

pGL3

pGL3

pGL3

pGL3

pGL3

pGL3

Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-423Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-424Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-425Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-426Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-427Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-428Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-429

pGL3

pGL3

Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-430Howard, L., Rex, M., Clements, D., Woodland, H. R.,2007. Regulation of the Xenopus Xsox17a1 promoter by co-operating VegT and Sox17 sites.Developmental Biology. 310, 402-431

CS107 amp

CS107 amp

pBF Amp

No ORF - 1.8kb

Fragment of NTPD2

clonned by digest into

pBF (SmaI/XhoI_

pBF Amp

pBF Amp

pBF Amp

pCS2+

pCS27

pBSRN3 Amp

Expression Clone

Expression Clone

pBSKS amp

pBSKS amp

pBSKS amp

pBSKS amp 3' UTR; 1.3kb

pGEMT easy amp 3' UTR; 0.5kb


Masse et al., 2006, Genomics




pGEMT easy amp 5' UTR; 0.5kb


pBSKS amp 3' UTR; 1kb

Masse et al., 2010, IJDB pGEMT amp

Masse et al., 2010, IJDB pBSKS amp






Coding Region, 0.66

kb. 1098-1757

3' UTR; 0.57kb; 2706-

3283

3' UTR; 0.42kb; 2727-

3154





Masse et al. in press, IJDB. pBSKS amp

Masse et al. in press, IJDB. pGEMT amp

Masse et al. in press, IJDB.

Coding Region, 0.58

kb. 1998-2585

5' UTR + Coding Region.

1.25kb; 96-1350

3' UTR; 1.8kb; 1066-1350

Coding Region, 0.58 kb. 598-1180

3' UTR; 0.88kb; 3620-

4500

3' UTR; 0.85kb; 1286-

2140

I.M.A.G.E EB473280 1.8kb, Full

Lenght Clone

Masse et al. in press, IJDB. pGEMT amp

Masse et al. in press, IJDB. pCMVSport6 amp

pcDNA3 amp

3' UTR; 0.5kb; 1399-1899I.M.A.G.E

BU913691 2.5kb, Full

Lenght Clone.

AGENCOURT_10493790

NICHD_XGC_OO1 Xenopus laevis cDNA

clone IMAGE:6640087 5-, mRNA

sequenceJust URF + 5'

UTR from I.M.A.G.E Clone into pcDNA3

EcorI/HindIII

Maya Piccinni:Got Picture

L911

Maya Piccinni: Got Picture

Maya Piccinni:Got PictureMaya Piccinni:Got Picture

L916

Maya Piccinni: Got Picture

Howard, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415

Original rapid excision

clone from lambda Uni-

Zap. Contains entire cDNA

as a 5’ EcoRI-3’ XhoI

fragmentHoward, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415

Coding region only PCR’d and cloned

into pSPJC2L. Xsox17a

cloned into Bgl II site,

Xsox17b into EcoRI siteHoward, L., Rex, M.,

Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415

Coding region only PCR’d and cloned

into pSPJC2L. Xsox17a

cloned into Bgl II site,

Xsox17b into EcoRI site

with endogenous (not globin)

3’UTR. Poorly translated


Fusion of the HMG domain (plus NLS) to the Engrailed

repressor domain

(which is at the C-

terminus), in pSPJC2L. Both

constructs made but

only Xsox17b:EnR used since it blocks both

Xsox17a and b


C-terminal truncation to

NLS generated by PCR- contains HMG domain

plus N-terminal

sequence – in pSPJC2LHoward, L., Rex, M.,


N-terminal truncation to

NLS generated by

PCR– lacks HMG domain – in pSPJC2L


Full coding region with a

C-terminal multiple myc tag. No UTRs In CS2+MT, so can be

used for DNA or RNA

injection



C-terminal fusion of the

VP16 activation

domain to the HMG domain (equivalent to EnR fusion). In pSPJC2LHoward, L., Rex, M.,


C-terminal fusion of the glutacorticoid

receptor ligand

binding domain to the full Xsox17a

coding region in pSPJC2L.

Xsox17b version not

madeHoward, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415Howard, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415Howard, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415Howard, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415Howard, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415


Howard, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415Howard, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415Howard, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415Howard, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415Howard, L., Rex, M., Clements, D., Woodland, H. R., 2007. Regulation of the Xenopus Xsox171 promoter by co-operating VegT and Sox17 sites. Developmental Biology. 310, 402-415


pSP64T amp

pCS2+ amp

pBluescript amp

pET14b amp

pET21b amp

pJC2L amp


The DNA binding

domain of VegT fused to

the VP16 activation domain.

Cloned into pST64T.

Transcripts were

generated by XhoI

digestion and SP6

transcription.


The ORF of VegT cloned in pCS2+.

A later clone (F3), as

above, of full length.


The ORF of Xpat with a

His tag at the N-terminus.


The ORF of Xpat with a

His tag at the C-terminus. Sense RNA

was transcribed from this

NotI-linearized

plasmid with SP6 RNA

polymerase. Xpat fused to

GST

The ORF of Xpat cloned into pJC2L. Sense RNA

was transcribed from NotI-linearized


polymerase.


pBluescript amp

pSP64T amp IB in pSP64T

pET-14b amp

pET-14b amp

pSPJC2L amp

pBSII-SK amp

pBSII-SK amp


IB in Bluescript


cDNA sequence encoding

amino acids 280-577 from

the xVelo1 ORF

subcloned into the

BamHI site of pET-14b.

Insert size 913bp.

Expressed protein used

to affinity purify goat anti xVelo1 polyclonal

antibody from serum.

cDNA sequence encoding

amino acids 205-269 from

the xVelo1 splice variant

ORF subcloned into the

BamHI site of pET-14b.

Insert size 217bp.

Expressed protein used

to affinity purify goat anti xVelo1

splice variant polyclonal

antibody from serum.

ORF of Xenopus

laevis Farnesyl

Transferase α-subunit

fused to eGFP (N-terminal

fusion). Insert subcloned

into the BgIII - HincII sites of

pSPJC2L.Pandur, P. D., M. L. Dirksen, K. B. Moore and S. A. Moody (2004). Xenopus flotillin1: a novel gene highly expressed in the dorsal nervous system. Developmental Dynamics 231: 881-887.


Pandur and Moody, 2000, Mech Dev. 96: 253-257.

aka 61B1; internal EcoRI

site! GenBamk Accession AF279254


pDH105 amp

pCS2+MT amp

Unpublished pCS4+MT Amp

Unpublished pCS2+ Amp

pXT7/eGFP Amp

pXT7 Amp

Pandur and Moody, 2000, Mech Dev. 96: 253-257.

The T7 site is mutated in the vector. There are

internal EcoRI and ApaI sites.


Brugmann et al., 2004 Development 131: 5871-

5881.Tag: Myc (6X, N-terminal)

Gene fragment: XKp78 /

XMARK3 ORF, PCR amplified

(pfu polymerase) as Xhol/Xbal

insert

Contains 345n of 5'UTR

sequence and open reading

frame of Xpar-1B Made by ligation of EcoRI/HindIII fragment of

5'RACE XPar1B

(5'UTR and open reading frame) and HindIII/NotI fragment of

pCS2+mycXPAR-1B

(coding sequence

only) untagged

eGFP+ XMARK3/Xpar-1A, wild type pXT7/eGFP

(Clontech) is described in Itoh et al 1999

MARK3 coding

sequence was PCR

amplified (Pfu polymerase)

with Bgl II/SalI primers using pCS2+ myc MARK3

WT as a template. Construct

was sequenced with T7 and mark3 KtoR

lower primersUnpublished and Kusakabe

et al 2004

Construct was

sequences with MARK3

Xbal stop primer and

ACT/T to GCT/A 560 mutation is

confirmed on midi prep

plasmid DNA. Mutation was

generated using a one-

step site-directed

mutagenesis on

corresponding plasmid

DNA template (Makarova,

2000 Kuskabe et al

2004)

pCS2+myc Amp

Unpublished pCS4+MT Amp

Edgar Pera pBluescript Amp

Edgar Pera pBluescript Amp

Tomas Pieler pCS2 Amp

Amp

Unpublished and Kusakabe et al 2004

Construct was

sequenced with MARK3

Xbal stop primer and mutation of ACT/T560 tp GCT/A was

confirmed on mini prep

plasmid DNAs

Gene fragment: XKp78 /

XMARK3 ORF, PCR amplified

(pfu polymerase) as Xhol/Xbal insert with

modified ATP-binding site

in kinase domain (K85

to R)

John Wallingford - hayes et al. Dev. Biol. 2007

pBluescript SK-

pCMV-Sport6 Amp

Toshikazu Nakamura pBS SK- Amp

pCS2+ Amp

pCS2+ Amp

pCS2+ Amp

pET16 Amp

Kyuno and Jones. Gene expression Patterns 2007

truncated c_RET lacking

the intracellular

domain

truncated c_RET lacking

the intracellular

domain

pBSK-globin Amp

pGEX-2T Amp

pSPG29 Amp

pGEM2

pGEX-2T

pGEX-KG

Xenopus cyclin A.

Xenopus cyclin A.

pinpoint2 biotinylated

pBSKII Amp

pET3B Amp

M13mp19 non specified

pET3b Amp

pBSKII Amp

pGEM2 Amp

T7β0 Amp

pGEM1 Amp

pT7-β-TAG Amp

pGEX-2T Amp

pBSKII Amp

pGEX-2T Amp

Xenopus cdc2 with a c-

myctag at its C-terminus in p-GEM1 (no

3' UTR)

C-terminally c-myc tagged Xenopus cdk2

in ESp11. Linearise with

NheI for transcription.

pSP64T Amp

pGEX Amp

pGEX-KG Amp

pGEX-2T Amp

pinpoint2 Amp

pGEX-KG Amp

pBSK Amp

pGEX-KG Amp

cs107 Amp mouse wnt3

pGEM3zf Amp Mouse wnt3a

pGEM7zf Amp mouse wnt5a

pBSK Amp mouse wnt7a

Amp

Amp Gli3 cDNA

mouse neurogenin 2

bluescript II KS

pbluescript Amp

CS107 amp

CS107 amp

CS107 amp

Hudson and Woodland (1998) Mech of Dev 73,

159-168

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

AL789158 - not full length - Tneu125J24

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp


CS107 amp

CS107 amp

CS107 amp

CS107 amp

amp

CS107 amp

pCMV-SPORT6.1



CS107 amp

pSP64T AMP

pCS2+ AMP

pCS2+ AMP

pSPT 18 Amp

Coding region of Xenopus

goosecoid in pSP64T.

Constructed by Jan

Christian by PCR

XL-1 host strain and

Amp resistant SP64T

goosecoid cloned and

characterized by lab of E. DeRobertis,

UCLA

mesodermal marker




pCS107 Amp TEgg085d20

Cell 67,1111-1120 Amp

EMBO J. 15 4547-4555 pBS Amp

AmppCMV-SPORT6

Image EST 4201740

Somites, pancreas



AmppCMV-SPORT6.1

Image 6643438. Accession BC084326



Amp

Amp

Amp source clone

pCS2+ Amp

pCMV-SPORT6

Image EST 5572834, Accession BC068655

pCMV-SPORT6


pCMV-SPORT6



Amp

Amp

Amp

Lavery et al., 2008. pCS2+ Amp Kidney

Garriock et al., 2007. pCS2+ Amp Kidney

pBLUESCRIPT KS



Ku and Melton, 1993. pT7TS Amp Kidney

Garriock et al., 2005. pT7TS Amp Kidney

pING14 Amp

pING14 Amp

pBSK Amp

pBSK Amp

Amp

Mouse BF-1 cDNA in pBSK

Brandli, Mechanisms of Development 1997 Dec 83-

104

Expressed on pronephric tubules and duct (stage 28). in later stages Paxb

is strongest in distal tips of nephrostome

s.



Amp

cs107-3STOP Amp GFP

Amp

CS4HA Amp

pBSKS- Amp

pCS105 Amp

pCS107 Amp

PCS1073STOP

MTNLS1073STOP, myc

NFAT luciferase

membrane tethered

dishevelled

Richer et al 88 PNAS 85:8086-8090. Richter

(1990) New Biol 2 556-565nrp1 lambda gt11 clone

X antipodean Xombi VegT brat T-box 6

1.8kb mouse proline rich

protein



Amp

pCS2+ Amp eGFP

pC105 Amp

pCS2+ Amp

pCS2+ Amp

?

? ?

dsh mitochondrial

Csbcatenin ESDC beta

catenin

expression of disheveled

mRNA


mRNA

ESP 11 Myc-tagged



? ?

pBSK Amp

pBSK Amp

pCS2+ Amp

pSP64TS Amp

pBSK2+ Amp

p53 activity is essential for normal development in

Xenopus.Wallingford JB, Seufert DW,

Virta VC, Vize PD.Curr Biol. October 1, 1997;

7 (10): 747-57.



Original Author




Antisense size

Dan Mahony (Gurdon's

lab)

DLP 1.3kb EcoRI StuI T7

Horst Grunz EcoRI T7

Clare Hudson 1.2

Mike Jones

BamHI T7

Branco Latinkic

Branko Latinkic

Liz Jones 1.8 kb SmaI/XhoI

Liz Jones

Liz Jones

Liz Jones

Liz Jones

3KB Fragment

clonned by digest into

pBF EcoRI/XhoI

Liz Jones

Liz Jones

Liz Jones

Liz Jones 1.3 kb KpnI T7

Liz Jones 0.5 kb PstI T3

Liz Jones 0.7 kb EcoRI T3

Liz Jones 1.4 kb SacI T3


Liz Jones 0.7 kb BamHI T7

Liz Jones 1 kb HindIII T7

Liz Jones 0.66 kb SacII SP6

Liz Jones 0.57 kb SacII T3

Liz Jones 0.42 kb SacII T3


Liz Jones 1.25 kb NcoI SP6








Liz Jones 1.7 kb

EcoRI T7


EcoR1 T7

Hugh Woodland

Hugh Woodland

Hugh Woodland

Hugh Woodland

Hugh Woodland

Hugh Woodland


913bp

217bp

NotI T7

Sally Moody NotI T7

Hugh Woodland

Hugh Woodland

Hugh Woodland

Hugh Woodland

Hugh Woodland


1.4 Kb Cloning Sites EcoRI, XhoI


NotI/SalI T3

XhoI/XbaI

EcoRI/NotI

Bgl II/SalI

Bgl II/SalI

Sally Moody / Petra Pandur

1.4 Kb Cloning Sites

NotI, XhoI

Sally Moody / Samantha Brugmann

1.4 Kb Cloning Sites

XbaI, KpnI

Olga Ossipova

Olga Ossipova

Olga Ossipova

Olga Ossipova

XhoI/XbaI

XhoI/XbaI

BamHI T7

BamHI T7

EcoRI T7

EcoRI T7

Olga Ossipova

Olga Ossipova

SalI T7

EagI SP6

EcoRI SP6

EcoRI T7

HindIII T7800bp Gli3 cDNA

NotI T7 ~7kb~4kb NotI and XhoI

Aaron Zorn

Aaron Zorn 1kb ClaI T7

EcoR1 SP6

Kenneth Kao SmaI/XhoI

EcoRI T7

De Robertis via Moon lab Jan Christian

850bp bglII BamHI

David Kimelman

EcoRI T7

EcoRI T7

NotI-SalI SalI or EcoRI T7

SalI or EcoRI T7Full length, NotI-SalI

SmaI or EcoRI T7

SalI T7

Liz Jones T7

Liz Jones T7

Full length, NotI-SalI


Liz Jones

Liz Jones

Liz Jones

Liz Jones

Liz Jones

Liz Jones

Liz Jones

Sfi T7

Peter Vize SacII T7

BF-1 cDNA between

EcoRI and XhoI

Xenopus cDNA pax-b -

pax2

1.2kb

2.2kb BamHI T3

Wenge Zhang

BamHI EcoRI

Tim Hunt

Tim Hunt

Tim Hunt

EcoR1

T7

EcoRV BamHI sp6

XbaI T3

Peter Vize - Steve Friend

Sense size Purpose Keywords OrganismSense Linearisation

Sense Polymerase

Sfi 1 / Pst 1 T3 in vitro RNA synthesis

Xho I SP6

Sma I SP6

in situ hybridisation

RNA for injection

RNA for injection

Xenopus tropicalis

Xenopus tropicalis

Sp6 NotI Xenopus laevis

Xenopus laevis

Xenopus laevis

Sp6 BgLI / NlvI

PvuI SP6 Rna Injection

PvuI SP6 Rna Injection

SfiI T3 RNA Injection

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

in situ clone

in situ clone

in situ clone

EcoRI T3 in situ clone

NcoI SP6 in situ clone

HindIII T7 in situ clone

BamHI T7 in situ clone

SacII SP6 in situ clone

SacI SP6 in situ clone

NotI T3 in situ clone

SacI T7 in situ clone

KpnI T7 in situ clone

KpnI T7 in situ clone



n/a T3 in situ clone




NotI SP6 in situ clone

Xenopus laevis

Xenopus laevis

Xenopus laevis


NotI SP6 in situ clone

Xenopus laevis

Xenopus laevis

Cell Transfection

(Protein Production)

Xenopus laevis

XhoI T3

XhoI SP6

XhoI SP6

XhoI SP6

XhoI SP6

XhoI SP6

NotI SP6


XhoI SP6

XhoI SP6


XhoI SP6 RNA Injection

RNA Injection

in situ probe

RNA Injection

NotI SP6 RNA Injection

NotI SP6 RNA Injection


X. laevis

X. laevis

XhoI SP6 RNA injection X. laevis

KpnI T3 In situ Probe X. laevis

XhoI T3 In situ Probe X. laevis

Protein Expression

Protein Expression


Asc1 SP6 X. laevis

BsshII SP6 RNA injection X. laevis

ApaI/NotI Sp6

Sp6

SalI T7

SalI T7

mRNA injection and

In Situ Hybridization

Overexpression

Overexpression

Overexpression

Overexpression

NotI Sp6

ApaI/NotI Sp6

WISH

WISH

WISH

WISH

Overexpression

Overexpression

WISH

WISH

NotI Sp6

NotI Sp6

NotI Sp6

mRNA synthesis

mRNA synthesis

mRNA synthesis

To generate probe for in

situ.

Mouse

Mouse

Mouse

XhoI T3 Mouse

Mouse

Xbal T3 Mouse

XhoI T3 XenopusWish probe or in vitro RNA synthesis

Xpat, XPAT, germ cell

probe

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

?

NotI SP6 Capped RNA

NotI SP6 RNA injection

Xenopus tropicalis


situ.

Xenopus laevis

KpnI T3

MafA XenopusTo generate probe for in

situ

HIPK2 XenopusTo generate probe for in

situ

Xenopus

NAB2 Tropicalis

EcoRI + XbaI SP6

EcoRI + XbaI SP6


situNrp2,

nueropilin


situ



NotI SP6

NotI SP6

To generate probe for In

situ


situ


situ

To generate mRNA for Injection.

Xenopus laevis


Xenopus laevis

XBaI T7

XBaI T7

RNA injection

Mouse


Xenopus laevis


Xenopus laevis

Xenopus laevis

Xenopus laevis

To genetare probe for in

situXenopus Laevis

Asc1 SP6

Not1 SP6 Mouse

Mouse

Xenopus Laevis


Mouse


sp6

HindIII/XhoI T7


situ and mRNA for injection.




Xenopus laevis




22 gata5

33 gata2

65 not-a notocord TF

89 rag1 n/a

91 xcat1

92 muc2

Gene synonyms



xGATA-5, xGATA5,


GATA-5, gata-4a


factor




factor

recombination activating

gene 1 [Predicted: Source is

Human Entrez Gene]

VDJ recombination


Ca2+-permeable

cation channel OSM-9 and

related channels

(OTRPC family)

mucin 2, oligomeric mucus/gel-

forming

xcg, xcg-1, xcg1, cg-1, cg1

von Willebrand factor and

related coagulation

proteins


Have to read paper to get info-Maya Piccinni

A1


135 muc2

163 kdr

164 aplnr

278 pSP6nucbGal

311

321 SuH

322 notch1 notch 1

mucin 2, oligomeric mucus/gel-

forming

xcg, xcg-1, xcg1, cg-1, cg1

von Willebrand factor and

related coagulation

proteinskinase insert

domain receptor (a

type III receptor tyrosine kinase)


Human Entrez Gene


vegfr2, xflk-1

recpeptor tyrosine kinase

(RTK)

apelin receptor

apj, apjr, hg11, X-msr, msr,

Xmsr, angio1, Xangio1, agtrl1



Beta-galactosidase

activin receptor


notch, xotch, xnotch, notch-

1, xnotch1signalling molecule


A12

Have to read paper to get info -Maya Piccinni

373 gata5 heart/gut TF

450 tbx4 limb TF

474 pax5 paired box 5 Pax-05 testis TF

533 rara

585 hcls1

723 spry2

755 n/a


xGATA-5, xGATA5,

xGATA-5a, xGATA5a, xGATA-5b, xGATA5b, GATA-5


xRAR alpha, rar alpha,

nr1b1 retinoid receptor

blood protein kinase

sprouty homolog 2

Xsprouty2, sprouty2, sprouty-2, sprouty 2,

Xtsprouty2, xspry2

FGF signal modulator

epb49 [Predicted: Source is

Human Entrez Gene]

erythrocyte membrane

protein band 4.9 (dematin)


Human Entrez Gene]

Actin-binding LIM Zn-finger

protein Limatin involved in

axon guidance

gene page can not be accesed-Maya Piccinni

no usage-Maya Piccinni

B19

gene page can not be accesed -Maya Piccinni

791 cbfa2t3 TF

798 etv6 ets variant 6 TF

800 ankra2

801 flt4 RTK

806 nr2f5

834 TF

837 n/a

843 casp3



translocated to, 3


mgc146892, TEL oncogene

ankyrin repeat, family A

(RFXANK-like), 2

structural muscle protein

fms-related tyrosine kinase

4vegfr3, vegfr-

3, flt-4

nuclear receptor

subfamily 2, group F,

member 5

coup, xCOUP-TFa,

xCOUPTFa, COUP-TFa, COUPTFa

hormone recptor

fam20a [Predicted: Source is

Human Entrez Gene]

family with sequence

similarity 20, member A [Predicted: Source is

Human Entrez Gene]


protein

caspase 3, apoptosis-

related cysteine

peptidase

CASP-3, Apopain, xCPP32,

caspase 3, caspase-3

apoptotic cysteine protease


A23

no usage -Maya Piccinni

845 casp9

869 c-fes, fps tyr kinase

873 mafa ripe3b1 TF

874

880 ptgs2

881 ptgs1

903 pim1 pim-1, pim

905 mll3 n/a

caspase 9, apoptosis-

related cysteine

peptidase

xCaspase-9, caspase-9, caspase 9, caspase9

apoptotic cysteine protease

fes [Predicted: Source is

Human Entrez Gene]

feline sarcoma oncogene [Predicted: Source is

Human Entrez Gene]

v-maf musculoapone

urotic fibrosarcoma

oncogene homolog A [provisional]

Gp1ba gene not there

platelet surface proteinprostaglandin-

endoperoxide synthase 2

(prostaglandin G/H synthase

and cyclooxygenas

e)

cox2, cox-2, phs-2, pgg/hs,

pghs-2, gripghs

peroxidase / oxygenase

prostaglandin-endoperoxide

synthase 1 (prostaglandin G/H synthase

and cyclooxygenas

e)

cyclooxygenase 1, cox1

peroxidase / oxygenase

pim-1 oncogene [Predicted: Source is

Human Entrez Gene]

serine/threonine

kinase


906 ankrd1 n/a Ankyrin repeat

909 znf385a n/a

923 rasgrf2 grf2, ras-grf2

1091-AP olig2 n/a unknown

109-SPH fzd7

1100-AP TF

114-KL dll1 delta-like 1

ankyrin repeat domain 1 (cardiac muscle)


Human Entrez Gene]

beta-1,6-N-acetylglucosaminyltransfera

se, contains WSC domain

Ras protein-specific guanine

nucleotide-releasing factor 2

guanine nucleotide exchange

factor

oligodendrocyte lineage



X-delta-1, delta1, delta-1,

Xdelta-1, XDelta1, x-

delta, Delta-1, Xdelta1

Notch signal modulator

124-SPH fzd9

149-KL n/a enzyme

14-SPH not known

150-KL nog noggin

16-ER

16-KL GFP TF

178-SPH gjb2 connexin

frizzled-9, frizzled 9, XFz9


glb1 [Predicted: Source is

Human Entrez Gene]



Human Entrez Gene]


nog1, noggin-1

extracellular BMP

antagonist

metallopeptidase

cx26, connexin-26, connexin 26,

dfna3, dfna3a, dfnb1, dfnb1a,

nsrd1, ppk

184-SPH cytochrome

18-ER fgf3 fgf-3, xfgf3

19-ER fgf8

223-SPH lef1

250-SPH lef1

26-ER hoxa9 homeobox A9

313-AP rbpj


signalling molecule


(androgen-induced)

mgc79739, Xfgf-8, Xfgf8, FGF-8, fgf8a, fgf8b, fgf-8b

signalling molecule

xlef-1, lef-1, xlef1


lymphoid enhancer-

binding factor 1

xlef-1, lef-1, xlef1


hoxa9-A, XlHbox6, XIHbox 6, XlHbox-6, xhoxa9

homeodomain transcription

factor


protein for immunoglobuli

n kappa J region

X-Su(H), XSu(H), Su(H), suppressor of

hairless, rbpsuh

signal transduction/notch signaling

pathway

31-ER ins insulin

355-AP TF

409-AP foxg1 TF

410-SPH wt1 xwt1, wt-1 TF

414-AP

445-AP snai2 neural plate TF

52-SPH undefined nuclear b-gal

preproinsulin, insulin I, ins1, xins, insulin

peptide hormone

forkhead box G1

XBF-1, foxg1b, bf1, XBf1

myh4 [Predicted: Source is

Human Entrez Gene]

myosin, heavy chain 4, skeletal muscle


Human Entrez Gene]

myh2b, myhc-2b, myhc-iib,

myhc E3, mhc, myhe3, MHC E3, MHC A7

cell surface protein

snail homolog 2


XSnail2

560-AP ccnb1 cyclin B1 cyclin B1, ccnb cyclin

562-AP cdh1 cell adhesion

56-KL n/a enzyme

570-AP cdh3 cell adhesion

64-NP six2 n/a TF

70-KL otx2

710-AP ptf1a pancreas TF

cadherin 1, type 1, E-cadherin

(epithelial)

uvo, cdhe, ecad, lcam,

arc-1, cd324, e-cadherin, uvomorulin

glb1 [Predicted: Source is

Human Entrez Gene]



Human Entrez Gene]

cadherin 3, type 1, P-cadherin

(placental)

xb-cad, cdhc-A, XB-cadherin,

XBcad, c-cadherin,

cdhp, hjmd, pcad, EP-

cadherin, b-cadherin

SIX homeobox 2


Xotx2, otx-2, Xotx-2

homeodomain transcription

factor

pancreas specific

transcription factor, 1a

Ptf1a/p48, XPtf1a



E67


E69


711-AP ins insulin

746-AP

85-SPH fzd9

86-KL neurog1 neurogenin 1

AP-46 cdk5 n/a cyclin activator

CS-328 cytoskeleton

CS-333 hoxb9 homeobox B9 TF

preproinsulin, insulin I, ins1, xins, insulin

signalling molecule

growth regulator


frizzled-9, frizzled 9, XFz9


neurogenin, ngn,

neurogenin1, neurogenin-1,

neurod3, ngn1, neurogenin 1




xhoxb9, hox 2.5



ER-29 igf1 growth factor

HAR-100 a2m TF

HAR-101

HAR-102

HAR-104 cytoskeleton

HAR-105 NO DNA

HAR-108 Gut/heart TF

insulin-like growth factor 1 (somatomedin

C)

IGF-I, Somatomedin,

xigf1, igf-1, somatomedin-

C

alpha-2-macroglobulin [provisional]

endodermin, Alpha2-

macroglobulin, endod, Edd,

edd

structural protein


metabolic enzyme



HAR-109 No DNA TF

HAR-110 Gut/heart TF

HAR-121 TF

HAR-125 TF

HAR-126 TF

HAR-141 anterior TF

HAR-146 wnt signalling modulator



HAR-149

HAR-151 frzb2

HAR-155 TF

HAR-163 foxn3 TF

HAR-168 ventx2.2 TF

HAR-169 TF

HAR-17 somite TF



[provisional]

crescent, frizzled related

proteinwnt signalling

modulator

forkhead box N3

ches1, checkpoint

suppressor 1

VENT homeobox 2,

gene 2 [provisional]

vent-2, vent2, xvent2, xvent-

2



HAR-188 TF

HAR-189

HAR-190 TF

HAR-191 foxj1.2 TF

HAR-196

HAR-211 neural TF

HAR-215 TF

TGFb signal modulator

signalling protein



HAR-218 TF

HAR-220 RTK

HAR-222 TF

HAR-223 TF

HAR-230 TF

HAR-235

HAR-265

iron binding protein

nodal1 [provisional]

nodal homolog 1

[provisional]

nr1, Xnr-1, Xnr1, nodal-

related 1, induction of

axial mesoderm,

nr1-a

signalling molecule



HAR-268 TF

HAR-273 TF

HAR-278

HAR-280 TF

HAR-281 delta, lmpy

HAR-287

HAR-291

intracellular signalling

psmb6 [Predicted: Source is

Human Entrez Gene]

proteasome (prosome, macropain)

subunit, beta type, 6


Human Entrez Gene]

signalling molecule

signalling molecule

structural protein



HAR-295 spry4

HAR-296 RTK

HAR-309

HAR-352 fus mgc52724 zinc finger

HAR-357 ihh

HAR-361 wnt4

HAR-366 n/a

sprouty homolog 4

sprouty4, sprouty-4, sprouty 4

FGF signalling modulator

TF and RNA splicing

fused in sarcoma

Indian hedgehog

X-BHH, banded hedgehog, bhh, X-hh,

indian hedgehog,

Xbhh

intercellular signaling

wingless-type MMTV

integration site family,

member 4

Xwnt4, Xwnt-4, wnt-4

secreted growth


regulator

chd2 [Predicted: Source is

Human Entrez Gene]

chromodomain helicase DNA

binding protein 2 [Predicted:

Source is Human Entrez

Gene]

Chromodomain-helicase DNA-binding protein



HAR-367 chd3 n/a helicase

HAR-37 neural TF

HAR-389 n/a

HAR-390

HAR-395 gzf1 n/a

HAR-397 fgf3 fgf-3, xfgf3

HAR-402

chromodomain helicase DNA

binding protein 3

krt10 [Predicted: Source is

Human Entrez Gene]

keratin 10 [Predicted: Source is

Human Entrez Gene]


kdr [Predicted: Source is

Human Entrez Gene]

kinase insert domain

receptor (a type III

receptor tyrosine kinase)


Human Entrez Gene]

flk1, flk-1, vegfr-2, vegfr2


GDNF-inducible zinc finger protein

1



heparin-binding growth

factor

pvrl2 [Predicted: Source is

Human Entrez Gene]

poliovirus receptor-related 2

(herpesvirus entry mediator B) [Predicted:

Source is Human Entrez

Gene]

cd112, nectin 2, nectin-2, hveb, prr2,

pvrr2

Predicted transmembran

e protein of the

immunoglobulin family of cell

adhesion molecules



HAR-426

HAR-428

HAR-430

HAR-434

HAR-435

HAR-436

HAR-442



HAR-446 tbx1 T-box 1

HAR-447 dlx5 dll3, X-dll3 0

HAR-67 TF

HAR-81 Neural crest TF

HAR-84 TF

HAR-85

HAR-92 zic2 neural crest TF

xtbx1, cafs, cthm, dgcr, dorv, vcfs,

tbx1c

T-box transcription

factor


secreted signalling

Zic family member 2

Zinc finger protein of the cerebellum 2, xzic2, hpe5



HAR-95 No DNA

HAR-98 cytoskeleton

HW-15

HW-18 TF

HW-27

JW-1205

JW-2040

signalling molecule

receptor complex

germplasm formation


fluorescent protein



JW-2113 mapt

JW-2277

JW-2377

JW-2378

JW-2492 Ca binding

LJ-10 cndp2

LJ-28

microtubule-associated protein tau

xtp, tau-like protein, tau

fluorescent protein

fluorescent protein

utrn [Predicted: Source is

Human Entrez Gene]

utrophin [Predicted: Source is

Human Entrez Gene]

utrophin, drp, dmdl, drp1

fluorescent protein

utrn [Predicted: Source is

Human Entrez Gene]

utrophin [Predicted: Source is

Human Entrez Gene]

utrophin, drp, dmdl, drp1

fluorescent protein

CNDP dipeptidase 2 (metallopeptid

ase M20 family)

cn2, cpgl, darmin-r

metalloprotease

signalling molecule



LJ-30

LJ-31 kidney TF

LJ-36

LJ-63

LJ-66

LJ-68 entpd3

LJ-69 n/a

clcnkb [Predicted: Source is

Human Entrez Gene]

chloride channel, voltage-

sensitive Kb [Predicted: Source is

Human Entrez Gene]

clckb, clk-k2, clc-kb, xClC-k, ClC-k, clcnka

chloride channel

signalling molecule

fluorescent protein

phosphodiesterase


diphosphohydrolase 3


Human Entrez Gene]

phosphodiesterase

enpp6 [Predicted: Source is

Human Entrez Gene]

ectonucleotide pyrophosphatase/phosphodie

sterase 6 [Predicted: Source is

Human Entrez Gene]

signalling molecule



LJ-70 entpd6 ion channel

LJ-71

LJ-72

LJ-77

LJ-82

LJ-83 nucleotidase

LJ-84 enpp2


Human Entrez Gene]




Human Entrez Gene]

phosphodiesterase

phosphodiesterase


Human Entrez Gene]




Human Entrez Gene]

phosphodiesterase


phosphodiesterase



LJ-85

LJ-86 TF

NP-A smarce1 DNA binding

NP-D ccnh cyclin H n/a

SM-10 TF

phosphodiesterase

SWI/SNF related, matrix

associated, actin

dependent regulator of chromatin,

subfamily e, member 1

baf57, mgc69429, mgc52696, mgc81726,

XBaf57




Probe name Plasmid name Pictures Atribution

n/a pButXG5FEnMT

Not in xenbase MG600 MG600

xnot Xnot2 not needed p

pPH16 v2 p p

Not in xenbase pCATBASIC

XCG-1 n/a p

Xenbase Number

XGATA5 fingers myctag

XB-CLONE-8596613

XB-CLONE-11536615

XB-CLONE-14586089



XCG1 n/a p

not in xenbase Xflk-1 flk 8-2 n/a p

Xmsr Xmsr p

Not in xenbase

n/a not needed p

GR-Su-H-VP16 not needed p

GR-NotchICD not needed p

XB-CLONE-11966505

XB-CLONE-12271664

XB-IMG-78380; need more?

CS2 deltaALK3 ITR No4

XB-CLONE-12271737

XB-CLONE-12271646


not needed p

tbx4 XB-IMG-78690 p

Pax5 pBS-14A6 no pictures p

RARalpha2 p

LCKBP1 no images p

Sprouty2 p

Dematin no pictures p

XB-CLONE-12440913

XGATA5-HA-pBut3

XB-CLONE-12441177

XB-CLONE-12441195

XB-CLONE-12441221

pBSXRARalpha2


XB-CLONE-2275742

IMAGE:4175828

XB-CLONE-2046366

IMAGE:5513422

have to upload them

XB-CLONE-2068494

IMAGE:6958304

gene page can not be accesed-Maya Piccinni

have to add it as synonim of gene-Maya Piccinni


G21

have to add it as synonim of gene -Maya Piccinni

Eto2-like no images p

etv6 no images p

Ankrd2 no images p

Flt4 Flt4-RZPD p

COUP-TFI p

Etsrp?

FAM20A no pictures p

Cas3 no pictures p

XB-CLONE-2154665

IMAGE:5156021

XB-CLONE-1851266

IMAGE:6929431

XB-CLONE-2192200

IMAGE:5571938

XB-CLONE-12441599

XB-IMG-48623; XB-IMG-48622

XB-CLONE-1607634

IMAGE:8317884

not yet in xenbase

Etsrp-pGEMTeasy

XB-CLONE-2029024

IMAGE:7010328

XB-CLONE-2151095

IMAGE:5084306



Sequenced as Trops-Maya Piccinni


G29


Cas9 p

Fes p

cMaf no pictures p

not in xenbase Gp1ba no pictures p

Cox2 yes p

yes p

Pim1a no pictures p

Mllt3 p

XB-CLONE-1658218

IMAGE:7976131

have to upload them

XB-CLONE-2039112

IMAGE:5516233

have to upload them

XB-CLONE-2267418

IMAGE:4743073

Gp1ba (4030892)

XB-CLONE-2126603

IMAGE:6864904

XB-CLONE-2021586

AminoTransferase

IMAGE:7008835

XB-CLONE-2165289

IMAGE:6640577

XB-CLONE-2227046

IMAGE:5078512

have to upload them





G32

add as synonim? -Maya Piccinni

I34


I35


Ankrd1 no pictures p

Zfp385 p

Rasgrp2 no pictures p

Xtolig2A Xtolig2A

pHSxfz7HSG pHSxfz7HSG ?

MSXI MSXI

no pictures p

XB-CLONE-1510907

Ankrd1 8742166

XB-CLONE-2040402

Zfp385 (5515527)


XB-CLONE-12441964

Rasgrp2 pGEM9480

XB-CLONE-14574073


bug is resolved

XB-CLONE-12442501

72-X-Delta-1H/R

72-X-Delta-1H/R



pCS2+ xfz9 pCS2 xfz9 not needed p

ngbgal CS2plus-nbgal not needed p

pT&TS wg ts

Xnoggin not needed p

not in xenbase Darmin no pictures

Otx2GFP Otx2GFP no pictures

pBSXenCo26

XB-CLONE-14574083

XB-CLONE-12442520

XB-CLONE-12442523

CS2plus xnoggin

pCDNA3-Darmin

XB-CLONE-2411396

IMAGE:2943095


bug is resolved



not in xenbase

not in xenbase XFGF3 pCS2 xFGF-3 no pictures

not in xenbase xFGF8 pCS2 FGF8 HA not needed

not in xenbase LVGR-MT LVGR-MT not needed p

pBS-SK-Lef1 pBS-SK-Lef1 no pictures

not in xenbase Xlhbox6

not in xenbase X Su ANK XSu-H-ANK not needed p

CArG Box1-cyto5-GFP

CArG Box1-cyto5-GFP

XB-CLONE-14574167

p not good with sp6

pGEM Xlhbox/Hoxb9

not in xenbase xInsulin xInsulin

not in xenbase Xmyc Xenopus Myc

XBF1 XBF1 ? p

not in xenbase WT1-27 WT1-27 no pictures p

not in xenbase xMHC pGEM-XMHC

not in xenbase Xslug pBSK-Xslug

not in xenbase pCMVnβgal pCMVnβgal

XB-CLONE-12440925


bug is resolved

X Cyclin B not needed p

not in xenbase CdhI pBSKplus-Cdh1 not needed

CS2+cbGal CS2 plus-cbGal not needed p

not in xenbase XcdhI no pictures

not in xenbase pBS-Six 2 pBS-Six 2 no pictures

pXOT30.1D no pictures p

XPtf1a/48

XB-CLONE-12442113

Xenopus Cyclin B 1-102

XB-CLONE-12442436

XcdhI-pBSKplus

XB-CLONE-12442474

pXOT30 1D-EcoRV

Xenopus Ptf1a/48 pGEM-T





F67


F69


XInsulin1

XGrr1 Xgrr1

no pictures p

Xngn Xngn-CS2MT no pictures p

Xp35.2 Fl Xp35-2 Fl no pictures p

not in xenbase XIF3 XIF3

xLHbox6 xLHbox6

Xenopus Insulin 1 pGEM-T

XB-CLONE-14573981

pBSXfz9(lambda1b)

pBSXfz9 - lambda1b

XB-CLONE-12442481

XB-CLONE-12271878

XB-CLONE-6095347





not in xenbase xIGF1

Endodermin p

not in xenbase Epidermal

not in xenbase Id2 id id2

not in xenbase tuba alpha tubulin

not in xenbase ODC odc ornithine

not in xenbase Gata4

Insulin like Growth Factor

1

XB-CLONE-4425642

IMAGE:5381340

trops 21/26/29-30

epidermal keratin keratin

gata gata4 gata-4





Not Available Endodermin

not in xenbase Gata6 gata gata-6

not in xenbase gbx2

not in xenbase dll2

not in xenbase hoxa7

not in xenbase hesx1 xanf1 xanf-1

not in xenbase sfrp-2

endodermin, edd

secreted frizzled related





sfrp-2

Crescent TNeu018n22 trops 13/21/26 p

egr2

Ches1 TGas027e12 Stage 23 p

vent-2 TGas019l23 n/a p

Foxi3

not in xenbase myod1

secreted frizzled related

XB-CLONE-4339951

krox20 krox-20, early

XB-CLONE-4130300

XB-CLONE-4135536

fkhd forkhead foxi3





emx3 emx emx3

Lef ty lefty antivin

msx2

FoxJ1 fkhd foxj1

Activin beta b activin

snai1 snail

Nkx

msx-2 xhox7.1' msx





Hb 9

FGF-8

Churchill churchill

irx1

VSX1 vsx-1 vsx1

cpl1

Xnr-1

hb9 xhb9 motor neuron

fgf-8 fgf8 fibroblast

xIro1 Iro1 iroquois1 iro-1

cpl1 cpl-1 lipocalin

Xnr1 Xnr-1 nodal related





Lbx1 lbx1

Xcad1

scap1

hes1

delta delta

e d n 1

cart1

xcad1 cad1 cad-1

skap55, src family

hairy2, hairy and

endothelin-1 endothelin1

cart1 cart-1 cartilage





spry4

Vegfr2

wt1

Fus- RBP

Ihh

Wnt4 Wnt4 Wnt-4

Chd2 xtChd2

sprouty4 sprouty-4

vegfr2 vegfr3 vegfr-2

wt-1 wt1 wilms tumor-1

Fus RNA BINDING PROTEIN

Indian Hedgehog Ihh





Chd3 xtChd3 not

snai2

kr t10

Vegfr2

GZF1

Fgf3 IMAGE

prpp2

cytokeratin, epidermal

vegfr2 vegfr3 vegfr-2

Growth factor induced

prpp2 poliovirus receptor





have to add it as synonim of the gene.-Maya Piccinni

G135

have to add it as synonim of the gene. -Maya Piccinni

let -7a

miR-7-2

miR-9a-2

miR-23b

miR-24a

miR-96

miR-219

microRNA let-7a

no accesion number

microRNA mir-7

no accesion number

microRNA miR-9

microRNA miR-23

no accesion number

microRNA miR-24

no accesion number

microRNA miR-96

no accesion number

microRNA miR-219






Tbx1 Tbx1

Dlx5 Dlx5

Gata-6

Zic5

Fkh5

XAG-2

Zic5 TGas041c07 n/a pXB-CLONE-4121510






FGF8

not in xenbase tubb (neural

pSPVegTGR pSPVegTGR

pJC2LGX8 pJC2LGX8

XdshD4

CS2-memRFP

accession number and clone name

same as HAR-90

neural specific tubulin

pCS2Grip2 MO+

pCS2Grip2 MO+


delete 894-1134

membrane-targeted fast-

folding monomeric

RFP






CS2-eGFP-tau eGFP-tau

eGFP-rGBD

CS2-GFP UTR

CS2-RFP UTR

Centrin2

not needed p

not in xenbase Nephrin nt2 Nephrin nt2

rGBD fused to eGFP for

visualizing active Rho in

vivo

Actin-binding region of utrophin


Xlt-Centrin2-GFP

XB-CLONE-2159421

DarminR I.M.A.G.E

BU993920

DarminR I.M.A.G.E

BU993920






not in xenbase Xclck Xclck no pictures

not in xenbase Pax 8 Pax 8

not in xenbase Xtwnt9b Xtwnt9b need doing

pCarGFP2 pCarGFP2

enpp4 T725 enpp4 T725

ntpd3 all pBF not neededentpd3 all pBF

enpp6 pcDNA3

enpp6 pcDNA3






ntpd6 pCS2+ not needed

entpd2 entpd2

entpd3 entpd3

p2Y8 PBF p2Y8 PBF

CD73 CD73

enpp2a pBF enpp2a pBF not needed

entpd6 pCS2+

entpd3 pCBC21

entpd3 pCBC21






entpd2 pcDNA entpd2 pcDNA

P2Y1 PBF P2Y1 PBF

SMARCE1 TEgg028f08 no pictures

CCNH TEgg041p20 no pictures

XB-CLONE-3968897

XB-CLONE-3987623

pCS2+VP16-FoxD5

pCS2+VP16-FoxD5






Sequence Paper Vector Antibiotics

p pBut2 Amp

pGem7 amp

amp

?? pBluescript KS- Amp

not sure BS+ Amp

Special Features

cloning vector sequence with T7F and SP6

3' end of XGATA-2B

cloning vector sequence with T3, M13R and

T7

pBluescript II KS+

not published yet, 08/97

I have used this for WISH

and it is OKish, see thesis

not god with T7

H. SiveCell 58, 171, 1985

p

Bluescript SK+

amp

p pCS2 Amp

pCS2 Amp

Cell 58:171-180

pUZ1 (Henikoff modified pBSM13;

Stratagene differentpolylin

ker)

homo sapiens aligthment but

not right protein

Cleaver et al., 1997. Dev.

Dyn. 210: 66-77.

homo sapiens aligthment but

not right protein

Devic et al., 1996. Mech.

Dev. 59: 129-140.


not good with SP6ot T3

Cloning Vector Sequence with

T7F

pour sequence with T7, no

sequence with SP6 or T3

Fu et al. (1998) Nature

Biotechnology 16: 253-257

CS2 deltaALK3 ITR derived

from pCS2-ITR (~4.5 kb)

ITR sequences are highly unstable

unless propagated in STBL2 E. coli

cells from GIBCO BRL.

Homo sapiens NR3c1

pBut3 Amp

pGem-T

Amp

pBluescript SK- Amp

not sure pCMV-SPORT6 Amp

pCMV-Sport6 Amp

Amp

no good read with t7

tft3sequence with sp6

cloning vector with M13R

vector sequence with

T7F

Heller and Brandli (1999) Dev Genet 24: 208-219

pBluescript II SK+

cloning vector with T7f t3

Kiode et al., 2001. Genes Dev 15: 2111-2121

IMAGE EST 4175828, Accession number

AAH60434

no aligment with SP6 or

T7F

Image EST 5513422. Full length cDNA,

Accession BC073605

pour sequence with SP6 and

T7FpCMV-

Sport6.ccdb

Image EST 6958304, Accesion

BC081247. Also called

Band 4.9 protei


pCMV-sport6 Amp

pDNR-LIB

pCMV-Sport6 Amp

pCMV-Sport6 Amp

pExpress1 Amp

pGEM-T Easy Amp

pCMV-SPORT6 Amp

Amp

no sequence aligment with T7F and CMVF

Image EST 5156021

xenopus ESR-4 with t7F and

M13FChloramphenic

olImage EST 6929431

not good with sp6 - wrong

gene

Image EST 5571938. Full

length. Accession BC078087

not exelent - tropicalis

Image EST 4970772. From

RZPD

no good with t7

Image EST 8317884

snai2 sequence

From Paul Krieg

Entire ORF lifted by PCR

from EST BC099054

no good read with t7f and

cmvf Image EST 7010328

comp42 sequence

CMV-SPORT6.ccdb

Image EST 5084306. Accession BC098991




K25

Sequenced as Trops -Maya Piccinni

pExpress1 Amp

not sure of seq pCMV-SPORT6 Amp

not sure of seq Amp



no sequence pCMV-SPORT6 Amp

pCMV-SPORT6 Amp

Amp

not good with T7. wrong

geneImage EST 7976131

Image EST 5516233, Accession BC073445.

Also known as Fps

pCMV-SPORT6.ccbb

Image EST 4743073.

Image EST 4030892. Accession BC157465

Image EST 6864904

Image EST 7008835

sequenced as pim3

Image EST 6640577

not good with t7

pCMV-SPORT6.ccdb






cv w m13rp pCS111 Amp


pGEMT-easy Amp

pTOPO

cv w t7 ?

Amp

pSP72 Amp X-delta-1

Image 87422166.

100% identical to image 6939850, Accession AAH99339

Image EST 5515527

cloning vector sequence with

T7 and SP6

Nagamine et al., 2008. BBRC 372: 886-891

From K Nagamine

neurotrophin receptor B

xTrkB-alpha (xTrkB)

cloning vector with t3 and t7

Xenopus laevis inhibitor of

DNA binding 3



described by Matt

-Maya Piccinni

pCS2+ AMP

pCS2+ Amp

pCs2+ Amp

pCDNA3 Amp Endoderm

Amp

pBSRN3 AMP

sequenced as fzd10-b

Two novel Xenopus

frizzled genes expressed in developing heart and

brain. Wheeler and hoppler 1999 Mech. Dev. 86 1-2

203-207

sCMV IE94 promoter

driving f3d9 expression.

AMP resistant. No IsceI-Tol2

sequence apparent.

Vector Sequence with

SP6 and T3

NLS plus b-galactosidease lacZ. Plasmid length 7278

bp.

sequenced as wg

(drosophila)

vector seq w sp6

Approximately 1 Kb insert

from noggin delta 5'. Hind3

site blunted and insert

ligated into CS2+ cut with EcoRI and StuI. StuI and Hind3 sites destroyed

in cloning.sequenced as

fgf3

Mech Dev (gene

expression patterns)3,

147-152

not good with SP6 or T7

Total size 6000bp.

Generated by inserting the 2.2kb SmaI - HindIII from

pXOtx2.2/nucbeta-gal into salI (blunt) -

HindIII digested

CXGFP3. Note: This GFP

construct has been modified by Jim Haseloff and Jonathan Pines to be

brighter and more stable

than wild type GFP.

sequenced as left-1

Expression cloning of Siamois, a Xenopus

homeobox gene

expressed in dorsal-vegetal

cells of blastulae and able to induce

a complete secondary

axis.Lemaire P, Garrett N,

Gurdon JB.Cell. 1995 Apr

7;81(1):85-94.

Xenopus Connexin 26 - IMAGE:294309

5



described by Matt

-Maya Piccinni

O45

described by Matt -Maya Piccinni

no good read pBSK AMP

pCS2+ Amp

unpublished pCS2+ Amp

? AMP

pBSSK AMP

cv w sp6 pGEM Amp

pCS2+ Amp

CArG Box1 oligos cloned

in KpnI

no food sequ w sp6

Xenopus Fibroblast

growth factor 3

sequenced as fgf4

Fibroblast growth factor 8

expression vector

no sequence with sp6;

Homo sapiens glucocorticoid

nuclear receptor variant 1

(NR3C1) T7F and t3

xLEF-1 without beta-cat binding

domain fused with 6X myc-tag, VP16 AD and human

GR-HBD (NheI + XbaI cut

TVGR)

sequence cdk5r1

Differential expression of the HMG box transcription factors XTcf-3

and XLef-1 during early

xenopus development.Molenaar M,

Roose J, Peterson J, Venanzi S, Clevers H, Destrée

O.Mech Dev. 1998 Jul;75(1-

2):151-4.

1650bp fragment of XI-

Lef1

Development 109,225-234

Sequenced as Xenopus laevis

notch 1 (notch1)

pCS2+ Amp

Pnt 3F ??

p ? ?

? ?

p pGEM

pBSK Amp

not sure of seq amp

19th down in the list

Dev Cell 1,655-665

no sequence with T7 sp6

and t3

no sequence with T7F - bacterial

sequence with SP6

Sequenced as Six3 protein

Positive and Negative

Regulation of Muscle Cell Identity by Members of

the hedgehog and TGFb

Gene Families

Du et al, 1997, J. Cell

Biology

Jim Du (Moon Lab)

pGex4T3 Amp

pBSK+ Amp

pCS2+ Amp

pBSK+ Amp

Amp

pBSKS- Amp xotxA otx2

cloning vector pGEM-T Amp

no sequence with T7 - cv with t3 and

pgex3 and sp6

sequenced as Xenopus laevis


(fzr1)

Cloning vector with SP6 and

t3

Xenopus laevis b-

galactosidase. The whole plasmid is

7162bp. This vector

produces a bgal protein

that is localized to the cytoplasm. It can be used

either to produce bgal

as a marker, or to construct bgal fusion

protein. Such proteins are

often functional. The

bgal sequences

start with the 9th amino acid

of the wild-type bgal

protein. The EcoRI site normally

present in the lacZ (bgal)

gene has been removed by a

silent point mutuation. The stop codon has

also been removed to

allow C-terminal

fusions to bgal. Without any insert, the

polylinker adds 13 amino acids

to the C terminus of

bgal. This does not appear to

alter the function of

bgal. Note that C terminus of

the lacZ is derived from

pPD16.43 (Fire, A et al 1990, Gene

93: 189-198)

Sequenced as Xenopus laevis


(fzr1)

vector w t3 and t7f and

m13rpBluescript II

SK+

cloning vector sequence with

t3 and t7





cloning vector pGEM-T Amp

fbxl20 pCS2+ Amp

pBSK+ AMP

pCS2MT Amp

pCS2+ Amp

bacterial seq pBluescript Amp

pGEM Amp

Sequence as Xenopus laevis frizzled family receptor 10 (fzd10-b)

Two novel Xenopus frizzled genes expressed in developing heart and brain. Wheeler and hoppler Mech. Dev. 86 1-2 203-207

not full length lacks 5' end and initiation ATG use for

insitu

neurog2 sequence

neurogenin xngn-1a

cloning vector sequence -

Wnt8a sequence with

SP6

cv seq sp6and t7f





cv pCS2+ Amp

amp

CS108 amp

CS108 amp

CS107 amp

CS107 amp

CS107 amp

Dev Cell 1,655-665

sequence not sactifactory

pCMV-SpPORT6.ccdb

dbEST Id: 12638199 - EST name:

NISC_no19b07.y1

GenBank Acc BQ526904 - GenBank gi:

21385773CLONE INFO

Clone Id: IMAGE:538134

0 (5')Source: IMAGE

Consortium, LLNL not full length

sequence as axial

protocadherininsert cloned into sal I-Not I

sequence as cell division

cycle associated 7

sequence as SMAD 1

sequence as zebrafish





CS107 amp

amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

Sequenced as embryonic

protein UVS.2-like

pCMV-SPORT6.1

no good sequence with

T7F

no sequence with T7F

sequenced as hoxb4

sequenced as Gata-6

Sequenced as Xenopus (Silurana)

tropicalis HESX homeobox 1

(hesx1)





p CS107 amp

CS107 amp

CS107 amp

CS107 amp

p CS107 amp

CS107 amp

CS107 amp

cone-rod homeobox sequence

AL638537 - Tneu018n22

3' end appears to be chimeric

with translation

initiation factor

sequenced as cloning vector

w/t7

sequenced as forkhead box

I4Tgas027E12,

AL647335

Tgas019L23 - AL631613 - not

full length

sequenced as foxi2,

accession number given refers to foxi2

name foxi3?

sequenced as mmp1

Myod myod - 3' present - AL783402





CS107 amp

CS107 amp

CS107 amp

amp

CS107 amp

CS107 amp

CS107 amp

sequence and accession as

emx1

sequence X.laevis 28S

ribosomal RNA gene for 28S

rRNA

sequence Xenopus laevis alpha-tubulin

gene w/t3

keratin 18 sequence

pCMV-SPORT6.1

wrong gene (BMP4)

sequence as vent 2

sequenced as rax / bmp4





CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

p CS107 amp

CS107 amp

sequenced as multiple genes

- very pour read

sequenced as xolloid

sequenced as grhl2

sequenced as xnkx2.5

vector sequence w t7f

sequence as different gene





CS107 amp

wrong gene CS107 amp


CS107 amp

CS107 amp

no alightment CS107 amp

CS107 amp

Cloning vector with t3

accession in database as

foxf1, sequence as

hex4

Sequenced as hes4

no good alightment





CS107 amp

CS107 amp

CS107 amp


CS107 amp

CS107 amp

CS107 amp

no good alighment

no good alighment

no good aligment

no good aligment

vector sequence w t3






CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

p CS107 amp

pcmvSport6 amp

sequence as cox1,

accession given in

database as chd5

Homo Sapiens NR3C1 with T7 / SP6 no

seq

Tneu006i21 - AL637273 - 5'

presentLIkely have 3'

also

no good alighment

Sequenced as Monodelphis

domestica PHD finger protein

15 (PHF15)

sequenced as tropicalis TM2

domain containing 2

(tm2d2)

accession in database as

pvrl2, sequence as

cyc1






p PCRII-TOPO amp

p PCRII-TOPO amp

CS107 amp

PCRII-TOPO amp

PCRII-TOPO amp

PCRII-TOPO amp

pCRII-TOPO amp

no good sequence

sequenced as mir 17b



vector sequencew

sp6






cv with t3 CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp

CS107 amp AL654653

no good read with t3 ot t7f

sequenced as VENT-2

gata gata6 gata- 6 - Genbank

#BG885303 - Full Length

sequence as ZIC-2

12S ribosomal RNA gene

sequence as ag1

sequence as zic2






CS107 amp

amp

pCS21MT amp

pSPJC2L amp

pSPJC2L amp

pCS2+ Amp

EGFP seq pCS2+ Amp

AL642603 - Tneu007i10

sequenced as ratus Ptch1

pGEM-5Zf(-) 3kb

Not full length -

Clone #52 was partially digested with

Nde I (cuts about 21 bp into 18S RNA

sequence and then

somewhere in CS107 which

liberates all of tubulin flanked

on the 5'end with some CS107 and then on

the 3'end with 21 bp of 18S sequence). [Nde I also

cuts in n-tubulin. ] This fragment was

purified and then cut with

NcoI. NcoI-NdeI

ntubulin cloned into the NcoI-NdeI site of pGEM-5Zf(-) 5' end is at

NcoI site- 3'end is NdeI

site

Kirilenko, P., Weierud, F. K., Zorn, A. M. and Woodland, H. R. (2008). The efficiency of Xenopus primordial germ cell migration depends on the germplasm mRNA encoding the PDZ domain protein Grip2. Differentiation 76, 392-403.

The ORF of the Grip2 cDNA

containing the Grip2 MO

binding site in the 50 UTR, and without

the stop codon, was amplified by

PCR and cloned into the ClaI and BamHI

sites of a pCS21MT

expression vector using the primer

1LG2MOplusF 50-

atataGGATCCCTGTCTGAAAAGCAACGTGA-30, introducing a

BamHI site and the primer:

1LG2stoplessR 50-

AGCTTTAAATCGATGGAGAGTCTTTGTGCTTG

TCTGTG-30, introducing a ClaI site. RNA

was synthesized from NotI-linearized


polymerase

Homo sapiens NR3C1

sequence


Generated by PCR

amplification of the human glucocorticoid receptor ligand

binding domain (Tada et al., 1997), which was

cloned downstream of, and in-

frame with, the VegT coding

region in pSPJC2L.

Transcripts were

generated by XhoI digestion

and SP6 transcription.

vector seq with sp6


A GFP fusion with GFP at the

N-terminus. This RNA

localized to centrioles, but did not form germ plasm-like islands in

oocytes. Sense RNA made as

above.no good

sequencew/sp6

Rothbächer U, et al.EMBO J. 2000 Mar 1;19(5):1010-22.

PDZ mutant Dvl2. For


mRNAmissin gmost of PDZaxis-inducing

+/-membrane translocating

+/- phosphorylated +/-CHECK ORIGINAL DETAILED

INFO IN PLASMID BINDER

Membrane label






gfp seq pCS2+ Amp

pCS2+ Amp GTPase activity

gfp seq pCS2+ Amp Actin label

rfp seq pCS2+ Amp Actin label

pCS2+ Amp

pCS2+ Amp source clone

pCMV sport 6 Amp

Development. 2005

Oct;132(20):4599-610.

Microtubule label. For

capped RNA, linearize with

NotI and transcribe with SP6.There are some lethality

issues so Kristen usually injects about

100pg RNA/

blastomere. We use

50pg/injection. From Kristen

Kwanpour sequence

with sp6

J Cell Biol. 2005 Jan 31;168(3):429-39.

Cell Motil Cytoskeleton. 2007 Nov;64(11):822-32.

Cell Motil Cytoskeleton. 2007 Nov;64(11):822-32.

GFP sequene with sp6

Nat Genet. 2008 Jul;40(7):871-9.

basal body/centroso

me

Homo sapiens junctional adhesion

molecule-1 mRNA

Homo sapiens transcription

factor 3 (TCF3)






pBS Amp

Amp

cv w t3 pBSKS Amp

seq as enpp4 pCar Amp

seq as enpp6 pCS2+ Amp

pBF Amp

seq as entpd3 pcDNA3 Amp

sequenced as crescent

sequenced as WT1

Tételin and Jones, 2008

(unpublished).Partial coding

(432nt).

Modified by Haseloff and

Pines.

GFP driven under the

control of the cardiac actin

promoter.

Cloned by PCR into

BamhI/EcoRI sites of pCS2+. Insert details ORF of enpp4

seq as entpd2

MO can Bind to it. All cDNA

from I.M.A.G.E. clone, all coding

reageon OK + 5' UTRORF + 3' and

5' UTR of enpp6

(=1.7kb) Clonned by

digest xbaI/xbaI into

xbaI of pcDNA3






seq as enpp4 pCS2+ Amp

seq as enpp2 pcDNA3

seq as enpp4 pcDNA3

enptd1 seq

p2y1 seq pcDNA3

seq as enpp4 pBF Amp

Fragment of 18kb of NTPD6 (UTR + ORF) clonned into

pCS2+ digested by

EcoRI

Mus musculus ectonucleoside triphosphate


(Entpd2)

Expression Clone






enpp4 seq

enpp4 seq

pCS107 Amp TEgg028f08

eif3J gene pCS107 Amp TEgg041p20

not good seq with T7

vector seq with sp6






Original Author

Adam

Geoff and Matt 600bp EcoRI

HindIII T7

638 EcoR1 T3

Cement Gland H. Sive Sac1 or Not1 not sure

Expression pattern




David Kimelman

pst1-pvuII from Xnot cDNA. (~200bp)

Valerie Hohman in Lisa

Steiners lab

Good question!!!!

Cement Gland Hazel Sive EcoRI NotI T3

4.3 kb. Eco R1 Not I T7

Bam HI T7

Universal

Blood - Endothelium, Blood vessels, Endocardium

Cleaver et al., 1997.

Blood - Endothelium, Blood vessels, Endocardium.Early expression not endothelial

specific.

Margaret Saha sent by P.

Krieg.

Yunging Shi (Sylvia Evans'

lab)DeltaALK3 (~1

kb)

BamH1 T7

B-cells Nicole Heller XbaI T7

Blumberg lab NotI T7

Blood - Myeloid T7

T7

SmaI T7

Cloned into XbaI and XhoI sites of pBut3-

HA

396bp (accession number:

AB032943)pcr fragment of

Xenopus Tbx4 cDNA

~540bp XbaI-EcoRI


SalI or SmaI or EcoRI

Full length (~2.061kb),

NotI-SalISalI or SmaI or

EcoRI

Blood - VBI, neural tube, brain, eye

Full length,SalI-NotI

NotI-SalI T7

SfiI

Eyes, somites SalI or EcoRI T7

Blood vessels NotI-SalI SalI T7

EcoRV-NotI SmaI or EcoRI T7

1.1kb SpeI T7

SalI-NotI SalI or SmaI T7

Apoptosis SmaI T7

Somites, head, BA, PN, PD, neural tube

SalI or EcoRI or EcoRV


Endothelium, lymphatics



Apoptosis EcoRV-NotI EcoRV or SmaI T7

Vasculature SalI or SmaI T7

Blood - VBI NotI-SalI T7

Blood - Myeloid NotI-SalI SalI or SmaI T7

NotI-SalI SmaI T7


NotI-SalI SmaI or EcoRI T7

NotI-SalI EcoRV T7


SalI or EcoRV or EcoRI

Heart SmaI-NotI BamHI T7


PCR fragment SacII Sp6

BamHI/KpnI T7

EcoRI T3

XhoI T7

Vasculature - Blood vessels

described by Matt

-Maya Piccinni

2.1kb

Dave Turner EcoRI/HindIII

Endoderm BamHI SP6

constructed by Grant Wheeler

Julia Yang-Snyder, Moon

Lab

Lyle Zimmerman

described by Matt

-Maya Piccinni

ClaI T7

Oliver Destree XbaI T7

HindIII SP6

Hoppler Lab - Fei Liu

1650 bp EcoRI site

EcoRI T7

XhoI sp6

Peter Vize BamHI T7

BamHI T7

Jim Du

EcoRI/XhoI

EcoRI/XbaI HindIII T3

ApaI T3

0.2kB NotI T7

SalI T7

Dave Tuner's lab Dave

Turner and Jacqueline Lee

beta-galactosode,

the whole plasmid is 7162bp.

NotI T7

3Kb EcoRI Asp718 T7

HindIII T7

ClaI - XhoI T7

Grant Wheeler (Hoppler Lab)

EcoRI-XhoI EcoRI T7

R. Fletcher

R. Fletcher

A. Zorn

Invitrogen/IMAGE

clone/XGC clone





Aaron Zorn

Aaron Zorn

Aaron Zorn

T7

~1.5 kb(?) cuts in insert with

EcorI/NotI

IMAGE 2kb

Aaron Zorn

James McGann T7

James McGann T7

James McGann T7

James McGann

James McGann

IMAGE

A. Zorn 1kb insert SalI T7

A. Zorn 850bp EcoRI T7

Mustafa 1.6kb insert ApaI SP6

Hugh Woodland

Hugh Woodland

Hugh Woodland



delete 894-1134

membrane-targeted fast-

folding monomeric

RFP

Kristen Kwan eGFP-tau

Bill Bement

Bill Bement

Brian Mitchell Centrin2

Liz Jones

Liz Jones Sma1 T7

William Bement

rGBD fused to eGFP for

visualizing active Rho in

vivo



Liz Jones Not1 T7

Liz Jones Not1 T7

Kidney Liz Jones BamHI T7

Liz Jones Kpnl or Notl

Liz Jones notI

Liz Jones

Liz Jones 1.7kb XbaI

all cDNA EcoRI/XhoI

Liz Jones 1.8kb EcoRI

Liz Jones

Antisense size Sense size

EcoRI T3 polymerase

HindIII T7

Sense Linearisation

Sense Polymerase

2.2 kb

Not I

Not1 SP6

NotI SP6

SfiI T3 ~1.160Kb

Nco1 SP6

EcoRI T3

XhoI T3

NotI Sp6

T7

NotI Sp6

NotI SP6

SalI SP6

Asp718 SP6

SalI SP6

NotI or Asp718 SP6

NotI T7

HindIII T3

XbaI T3

NotI SP6

sp6

NotI SP6

AscI Sp6

Not1 SP6

XhoI SP6

NotI SP6

NotI/ SP6

not1 sp6

Not I/SP6

NotI/SP6

NotI/Sp6

NotI/Sp6

NotI/SP6

XhoI T3

Sp6

Sp6 PvuI

Sp6

PvuI SP6

Purpose Keywords Organism

Xenopus

RAG-1 WISH Xenopus

WISH Xenopus

in vitro transcription of

Xenopus GATA5 fingers myc tag

RNA

gata5, gata-5, gata 5, xgata5,

xgata-5, xgata 5, xenopus, myc tag, fingers, engrailed, dominant

negative,gata5en

RNase Protection

Xnot not flh Not

RAG1, RAG-1, thymus

XCG1, XCG-1, cement gland

in situ probe CG1, XCG1 xenopus

Xenopus

Xenopus

injection xenopus

injection xenopus


Xflk-1, Endothelium, Blood vessels, Endocardium, Xenopus, flk,

flk-1,Xflk1,flk1


msr, Xmsr, Endothelium, Blood vessels, Endocardium,G

protein, Xenopus

DNA Injections, transgenics

tBR, tBR-ITR, ITR,

transgenicsXenopus

(universal?)

GR-Su(H)VP16, VP16, GR

Notch, GR-NotchICD, GR

xenopus

xenopus

in situ probe Pax5, XPax5 Xenopus

RAR, RARalpha Xenopus

LCKBP1 Xenopus laevis

Xenopus

Xenopus


XGATA5-HA-pBut3,

Xenopus GATA5, GATA5

Tbx4, tbx4, Tbx-4, tbx-4

in situ probe and mRNA for

injection


situ


situSprouty2, Sprouty


situDematin, Band

4.9

Xenopus

Tel1, Tel Xenopus

Xenopus

Flt4, VEGR3 Xenopus

Xenopus

Etsrp? Xenopus

Xenopus

Xenopus


situ

Eto-like, MTGR1-like, Eto, MTGR1


situAnkrd2, Ankrd,

ankyrin


situ


situ

COUP-TFI, COUP-TF, COUPTF


situ


situFAM20, FAM20A


sistuCas3,

caspase3

Xenopus

Fps, Fes Xenopus

cMaf, Maf Xenopus

Gp1ba Xenopus

Cox2, Cox Xenopus

Xenopus

Pim1a, Pim3 Xenopus

Mllt3 Xenopus


situCas9,

caspase9


situ


situ


situ


situ


situAmino

transferase


situ


situ

Ankrd1 Xenopus

Xenopus

Rasgrp2 Xenopus


situ


situZfp385, zinc

finger


situ


situ.

Capped RNA ?

Xenopus laevisTo generate

mRNA for injection

NO USAGE ASIGNED

In situ Probe

NO USAGE ASIGNED

RNA Injection and Capped

RNA

In situ Probe

? ?

Xenopus tropicalis

?

Xenopus laevis

Xenopus laevis

Xenopus laevis

Capped RNA & In situ Probe





Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis

Xenopus tropicalis



Xenopus tropicalis

RNA Injection laevis

RNA Injection

RNA Injection

membrane RFP

Xenopus tropicalis

Xenopus tropicalis

for expression of disheveled mRNAmissin

gmost of PDZaxis-

inducing +/-membrane

translocating +/-

phosphorylated +/-CHECK ORIGINAL

DETAILED INFO IN PLASMID

BINDER

dsh xdsh dishevelled GFP partial PDZ delete

membrane labelling

eGFP, tau, gfp

For capped RNA, linearize with NotI and

transcribe with SP6.There are some lethality

issues so Kristen usually injects about

100pg RNA/blastomer

e. We use 50pg/injectionF

rom Kristen Kwan Rho sensor,

GBD, rGBD, gfp, rho

Utrophin, GFP, actin binding

Utrophin, RFP, actin binding

C-Term GFP fusion5'BamHI / 3'XbaI cloning

Centrin, GFP, Centrin2



situ

Xenopus laevis

RNA message Xenopus laevis


Xenopus laevis


situ


situ


situXenopus tropicalis


For Cell Transfection

RNA message Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

Xenopus laevis

RNA Injection Xenopus laevis

Xenopus laevis

Xenopus laevis

tropicalis

xxxxx sequence not yet uploaded to xenbase

laevis

means that the sequence supposed to be laevis but is actually coming up as tropicalis

means that the sequence supposed to be tropicalis but is actually coming up as laevis

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Transcript of [XLS]xenopusresource.org · Web view736. 866. 766. 310. 344. 763. 785. 883. 857. 803. 918. 788....