www.WilksIR.comwww.WilksIR.com

InfraCal TOG/TPH Analyzer, InfraCal TOG/TPH Analyzer, Model HATR-T2Model HATR-T2

Extraction ProcedureExtraction Procedure

Collect a measured amount of Collect a measured amount of samplesample

For samples that will be stored for For samples that will be stored for more than a day or for samples more than a day or for samples with a high pH:with a high pH:

Adjust the pH to less than 2 with Adjust the pH to less than 2 with Hydrochloric acid or Sulfuric Acid Hydrochloric acid or Sulfuric Acid (typically 3-5 drops depending (typically 3-5 drops depending on buffers in sample)on buffers in sample)

Add SolventAdd Solvent

10 parts sample to 1 part solvent

For example:For example:

For 150 ml of sample addFor 150 ml of sample add

15 ml of solvent 15 ml of solvent

Add SolventAdd Solvent

10 parts sample to 1 part solvent

For example:For example:

For 150 ml of sample addFor 150 ml of sample add

15 ml of perchloroethylene 15 ml of perchloroethylene

Shake for 2 minutesShake for 2 minutes

Open the cap on the bottle during Open the cap on the bottle during the 2 minute shaking to relieve the 2 minute shaking to relieve the pressure the pressure

Hexane is lighter than water and Hexane is lighter than water and will float on the top of the samplewill float on the top of the sample

Using a syringe or pipette, take 50 Using a syringe or pipette, take 50 microliters of solvent extractmicroliters of solvent extract

And deposit it in the center of the And deposit it in the center of the sample crystal sample crystal

Press the run button Press the run button

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Oil concentration displayed Oil concentration displayed

Silica Gel ExtractionSilica Gel Extraction

The difference between TPH (Total The difference between TPH (Total Petroleum Hydrocarbon) and TOG Petroleum Hydrocarbon) and TOG (Total Oil and Grease) is the polar (Total Oil and Grease) is the polar

organics are removed from the extract organics are removed from the extract using silica gel. The remaining using silica gel. The remaining hydrocarbons are the non-polar hydrocarbons are the non-polar

components considered to be TPHcomponents considered to be TPH

Silica Gel ExtractionSilica Gel Extraction

Put filter paper into funnelPut filter paper into funnel Add silica Add silica

Silica Gel ExtractionSilica Gel Extraction

Put filter paper into funnelPut filter paper into funnel Add silica Add silica Put funnel into bottlePut funnel into bottle Put solvent extract throughPut solvent extract through

silica gelsilica gel

Silica Gel ExtractionSilica Gel Extraction

Put filter paper into funnelPut filter paper into funnel Add silica Add silica Put funnel into bottlePut funnel into bottle Put solvent extract throughPut solvent extract through

silica gelsilica gel Using a 50 microliter syringe or Using a 50 microliter syringe or

pipette deposit on the center of pipette deposit on the center of the sample plate crystalthe sample plate crystal

Press the run button Press the run button

14

Oil concentration displayed Oil concentration displayed

For questions please contact us at:For questions please contact us at:tech@WilksIR.com, or 203-855-9136tech@WilksIR.com, or 203-855-9136

InfraCal TOG/TPH AnalyzerInfraCal TOG/TPH AnalyzerExtraction ProcedureExtraction Procedure