Www.emtech.upm.Edu.my Document EMTech Calendar

2014 CALENDAR

Celebrating the International Year of

Crystallography

Enzyme and Microbial Technology Research Centre

Enzyme and Microbial Technology Research Centre (EMTECH),

Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor,

MALAYSIA. Tel: +603-8947 1416 Fax: +603-8946 7590

FIRST SEMESTER

Classes 9 September 2013 - 3 November 2013 8 weeks

# Mid-semester Holidays 4 November - 10 November 2013 1 week

* Classes 11 November 2013 - 22 December 2013 6 weeks

# Study Break 23 December 2013 - 29 December 2013 1 week

Final Examination 30 December 2013 - 12 January 2014 2 weeks

- Breaks Between Semester 13 January 2014 - 16 February 2014 5 weeks

SECOND SEMESTER

## Classes 17 February 2014 - 6 April 2014 7 weeks

Mid-semester Holidays 7 April 2014 - 13 April 2014 1 week

Classes 14 April 2014 - 1 June 2014 7 weeks

Study Break 2 June 2014 - 8 June 2014 1 week

Final Examination 9 June 2014 - 22 June 2014 2 weeks

*** Final Semester Break 13 January 2014 - 16 February 2014 10 weeks

THIRD SEMESTER (SPECIAL)

Classes 23 June 2014 - 10 August 2014 7 weeks

Final Examination 11 August 2014 - 18 August 2014 1 weeks

SYMBOL DATE DAY

** Deepavali 2 November 2013 Saturday

# Christmas 25 December 2013 Wednesday

## Chinese New Year 31 January - 1February 2014 Friday & Saturday

*** Hari Raya Puasa 28 - 29 July 2014 Monday & Tuesday

UPM Academic Calendar

EMTECH Activity Calendar

Workshop on Manuscript Writing March

EMTECH Colloquium 2014 * May/June

Workshop on Protein Crystallography * November/December

* To be confirmed. Subject to change

EMTECH also offers service of renting equipments. For a full list of equipments,

please contact +603 8947 1416 for further information.

From left to right: Freeze dryer, spray dryer, bioreactor (7.5 L)

From left to right: YSI 2700 Select Biochemistry Analyzer, circular dichroism (CD), gel documentation

1 January 2014 (Wednesday) New Year Celebration All states except Johor, Kedah, Kelantan, Perlis &

Terengganu

14 January 2014 (Tuesday) Prophet Muhammads Birthday All states

17 January 2014 (Friday) Thaipusam Kuala Lumpur, Putrajaya, Johor, N. Sembilan,

Perak, Penang & Selangor

31 January 2014 (Friday)

1 February 2014 (Saturday)

Chinese New Year (Day 1)

Chinese New Year (Day 2)

All states except Kelantan & Terengganu

1 May 2014 (Thursday) Labour Day All states

13 May 2014 (Tuesday) Wesak Day All states

7 June 2014 (Saturday) Agongs birthday All states

15 July 2014 (Tuesday) Nuzul Al-Quran Kelantan, Pahang, Perak, Perlis, Penang, Selangor

& Terengganu

28 July 2014 (Monday)

29 July 2014 (Tuesday)

Hari Raya Puasa * All states

31 August 2014 (Sunday) Merdeka Day All states

16 September 2014 (Tuesday) Malaysia Day All states

5 October 2014 (Sunday)

6 October 2014( Monday)

Hari Raya Qurban *

Hari Raya Qurban (Day 2)

All states

Kelantan & Terengganu only

23 October 2014 (Thursday) Deepavali * All states except WP Labuan & Sarawak

25 October 2014 (Saturday) Awal Muharram (Maal Hijrah) All states

11 December 2014 (Thursday) Sultan Selangors birthday Selangor

25 December 2014 (Thursday) Christmas Day All states

* To be confirmed. Subject to change

2014 Public Holidays

21 March - 29 March

22 March - 30 March

Term 1 Holidays

Term 1 Holidays

Kedah, Kelantan & Terengganu

Perlis, Penang, Perak, Selangor, N. Sembilan, Melaka, Johor,

Pahang, Sabah, Sarawak, WP Kuala Lumpur, Putrajaya,

Labuan

27 May - 14 June

28 May - 15 June

Mid-year Holidays

Mid-year Holidays




Labuan

12 September - 20 September

13 September - 21 September

Term 2 Holidays

Term 2 Holidays




Labuan

21 November - 3 January

22 November - 4 January

End-year Holidays

End-year Holidays




Labuan

2014 School Holidays



2014

Sun Mon Tue Wed Thu Fri Sat

1 2 3 4

5 6 7 8 9 10 11

12 13 14 15 16 17 18

19 20 21 22 23 24 25

26 27 28 29 30 31

JANUARY 2014

S M T W T F S

1 2 3 4 5 6 7

8 9 10 11 12 13 14

15 16 17 18 19 20 21

22 23 24 25 26 27 28

29 30 31

S M T W T F S

1

2 3 4 5 6 7 8

9 10 11 12 13 14 15

16 17 18 19 20 21 22

23 24 25 26 27 28

DECEMBER 2013 FEBRUARY 2014

Public Holidays (January 2014):

1st - New Year 14th - Maulidur Rasul 14th - Yang di-Pertuan Besar Negeri Sembilan's Birthday 17th - Thaipusam 19th - Sultan of Kedah's Birthday 31st - Chinese New Year (1st)

T1 lipase protein crystal at different crystallization temperature: 16C (top), 20C (middle) and 40C. T1 lipase is the first protein crystal in Malaysia.

3D structure of T1 lipase


Reference:

Leow, T. C., Rahman, R. N. Z. A., Basri, M. and Salleh, A. B. (2007) High Temperature Crystallization of

Thermostable T1 Lipase. Crystal Growth & Design. 7: 406-410

Hiroyoshi Matsumura, Takahiko Yamamoto, Leow Thean Chor, Abu Bakar Salleh, Mahiran Basri, Tsuyoshi Inoue, Yasushi Kai, Raja Noor Zaliha Raja Abd Rahman (2008) Novel cation-p interaction revealed by crystal structure of thermoalkalophilic lipase, PROTEINS: Structure, Function, and Bioinformatics 70 (2): 592-598


1

2 3 4 5 6 7 8

9 10 11 12 13 14 15

16 17 18 19 20 21 22

23 24 25 26 27 28

FEBRUARY 2014

S M T W T F S

1

2 3 4 5 6 7 8

9 10 11 12 13 14 15

16 17 18 19 20 21 22

23 24 25 26 27 28 29

30 31

MARCH 2014

S M T W T F S

1 2 3 4

5 6 7 8 9 10 11

12 13 14 15 16 17 18

19 20 21 22 23 24 25

26 27 28 29 30 31

JANUARY 2014

Public Holidays (February 2014):

1st - Chinese New Year (2nd ) 1st - Federal Territory Day

National Angkasawan Programme:

Soyuz TMA-11 was launched to space on October 10th, 2007

and Soyuz TMA-10 landed on Earth on October 21st, 2007.

HDPCG (High Density Protein Crystal Growth)

was used for protein crystallization in space

with T1 lipase was used as model protein.

Ground crystal of T1 lipase was compared

with its space crystal.

Top: T1 lipase space crystal (1.35 ). Microgravity

crystallization produces better electron density

map thus an improved protein structure for better

understanding.

Top: T1 lipase ground crystal (1.7 ). Poor electron density map.



1

2 3 4 5 6 7 8

9 10 11 12 13 14 15

16 17 18 19 20 21 22

23 24 25 26 27 28 29

30 31

MARCH 2014 S M T W T F S

1

2 3 4 5 6 7 8

9 10 11 12 13 14 15

16 17 18 19 20 21 22

23 24 25 26 27 28

FEBRUARY 2014

S M T W T F S

1 2 3 4 5

6 7 8 9 10 11 12

13 14 15 16 17 18 19

20 21 22 23 24 25 26

27 28 29 30

APRIL 2014

Public Holidays (March 2014):

4th - Anniversary of Installation of Sultan of

Terengganu

Collaboration between EMTECH and JAXA (Japanese Aerospace Exploration Agency) to

grow protein crystals in Kibo or JEM (Japanese Experiment Module) that is designed

for microgravity experiment. It is a Japanese science module for the ISS developed by

JAXA. JEM is the first human-rated spaced facility for conducting scientific research

activities on orbit.

Six flights offered in three years from July 2009

until March 2013 with each flight duration between

2-4 months.

Top: One set of JCB-HD (JAXA crystallization apparatus)

contains 12 capillaries with 4 protein samples for 3 capillary

for each experiment.

T1 lipase

L2 lipase V171S

L42 lipase

D311E


APRIL 2014 Sun Mon Tue Wed Thu Fri Sat

1 2 3 4 5

6 7 8 9 10 11 12

13 14 15 16 17 18 19

20 21 22 23 24 25 26

27 28 29 30

S M T W T F S

1

2 3 4 5 6 7 8

9 10 11 12 13 14 15

16 17 18 19 20 21 22

23 24 25 26 27 28 29

30 31

MARCH 2014

S M T W T F S

1 2 3

4 5 6 7 8 9 10

11 12 13 14 15 16 17

18 19 20 21 22 23 24

25 26 27 28 29 30 31

MAY 2014

Public Holidays (April 2014):

15th - Declaration of Malacca as a

Historical City

18th - Good Friday

19th - Sultan of Perak's Birthday

26th - Sultan of Terengganus Birthday

L2 lipase ground crystals of two different shapes. Diamond

shaped (orthorhombic) (left) and rod-shaped (right).

L2 lipase space crystals. Orthorhombic shaped crystal is

the one in blue (left).

Solved 3D structure of L2 lipase (1.5 )

Reference:

Fairolniza Mohd Shariff, Raja Noor Zaliha Raja Abd. Rahman,

Mohd Shukuri Mohd Ali, Adam Thean Chor Leow, Mahiran Basri

and Abu Bakar Salleh (2010) Crystallization And Preliminary X-ray

Crystallographic Analysis Of A Highly Thermostable L2 Lipase From

A Newly Isolated Bacillus sp. L2. Acta Crystallographica Section F,

F66:715-717


MAY 2014

Superimpose of chains A and B for

ground-grown and space-grown of T1

lipase. (Top) Superimpose of chain A

for both T1 lipase crystal (magenta

for space crystal) with RMSD of

0.2185. (Left) Superimpose of

chain B (gray for space crystal) with

RMSD of 0.4214 .


1 2 3

4 5 6 7 8 9 10

11 12 13 14 15 16 17

18 19 20 21 22 23 24

25 26 27 28 29 30 31

S M T W T F S

1 2 3

4 5 6 7 8 9 10

11 12 13 14 15 16 17

18 19 20 21 22 23 24

25 26 27 28 29 30 31

APRIL 2014

S M T W T F S

1 2 3 4 5 6 7

8 9 10 11 12 13 14

15 16 17 18 19 20 21

22 23 24 25 26 27 28

29 30

JUNE 2014

Public Holidays (May 2014):Public Holidays (May 2014):Public Holidays (May 2014):Public Holidays (May 2014):

1st

- Labour Day

7th

- Hari Hol Pahang

13th

- Wesak Day

17th

- Raja Perliss Birthday

27th

- Israk & Mikraj

30th

& 31st

- Pesta Kaamatan Sabah

Ground-grown (top) and

space-grown (right) crystals

of T1 lipase in capillary

using counter diffusion

method.

Snapshots of ground-grown crystal (1.1) and space-grown crystal (1.3) electron density map countered at 1 sigma level displayed in COOT.

Reference:

Sayangku Nor Ariati Mohamad Aris, Adam Leow Thean Chor, Mohd Shukuri Mohamad Ali, Mahiran Basri, Abu Bakar Salleh, and Raja Noor Zaliha Raja Abd. Rahman, Crystallographic Analysis of Ground and Space Thermostable T1 Lipase Crystal Obtained via Counter Diffusion Method Approach, BioMed Research International, vol. 2014, Article ID 904381, 8 pages, 2014. doi:10.1155/2014/904381


JUNE 2014 Sun Mon Tue Wed Thu Fri Sat

1 2 3 4 5 6 7

8 9 10 11 12 13 14

15 16 17 18 19 20 21

22 23 24 25 26 27 28

29 30

Reference:

Rudzanna Ruslan, Raja Noor Zaliha Raja Abd. Rahman, Thean Chor Leow, Mohd Shukuri Mohamad Ali, Mahiran Basri, Abu Bakar Salleh (2012) Improvement of Thermal Stability via Outer-loop Ion Pair Interaction of Mutated T1 Lipase, International Journal of Molecular Sciences 13, 943-960

Public Holidays (June 2014):Public Holidays (June 2014):Public Holidays (June 2014):Public Holidays (June 2014):

1st

& 2nd

- Hari Gawai Sarawak

7th

- Agongs Birthday

29th

- Awal Ramadhan

Top: Diffraction patterns of thermostable mutant lipase D311E. The resolution is ~2.1 at the edge with starting position; distance: 50.00 mm, 2 theta: 22.00, Omega: 31.64, Phi: 271.48, Chi: 30.01 and ending position; distance: 50.00 mm, 2 theta: 22.00, Omega: 31.64, Phi: 271.98, Chi: 30.01. Bottom: D311E lipase crystal grown using sitting drop meth-od with 0.1 M MES pH 5.5, 0.1 M Sodium phosphate, 0.1 M Potassium phosphate, and 1.5 M NaCl as precipitant reagent.

S M T W T F S

1 2 3 4 5

6 7 8 9 10 11 12

13 14 15 16 17 18 19

20 21 22 23 24 25 26

27 28 29 30 31

JULY 2014 MAY 2014

S M T W T F S

1 2 3

4 5 6 7 8 9 10

11 12 13 14 15 16 17

18 19 20 21 22 23 24

25 26 27 28 29 30 31

Top: Snapshot of ion pair interaction formation of D311E lipase crystal structure at residue between Glu311 and four residues (Arg 274, Thr 278, Gly 279 and Arg 303).


Public Holidays (July 2014):

7th - Georgetown Heritage Day

12th - Penangs Governors Birthday

15th - Nuzul Al-Quran

28th & 29th - Hari Raya Puasa

S M T W T F S

1 2 3 4 5 6 7

8 9 10 11 12 13 14

15 16 17 18 19 20 21

22 23 24 25 26 27 28

29 30

JUNE 2014

S M T W T F S

1 2

3 4 5 6 7 8 9

10 11 12 13 14 15 16

17 18 19 20 21 22 23

24 25 26 27 28 29 30

31

AUGUST 2014


1 2 3 4 5

6 7 8 9 10 11 12

13 14 15 16 17 18 19

20 21 22 23 24 25 26

27 28 29 30 31

JULY 2014

Top: 3D Structure of S2F lipase

Top: S2F lipase space crystal

S2F is a mutant of thermostable L2, and was mutated at its N-terminal with the second amino acid, serine being substituted to phenylalanine.

Optimization of S2F lipase protein

crystal. Optimum precipitant at

0.75 M, and at pH 9.0



1 2

3 4 5 6 7 8 9

10 11 12 13 14 15 16

17 18 19 20 21 22 23

24 25 26 27 28 29 30

31

AUGUST 2014

S M T W T F S

1 2 3 4 5

6 7 8 9 10 11 12

13 14 15 16 17 18 19

20 21 22 23 24 25 26

27 28 29 30 31

JULY 2014

S M T W T F S

1 2 3 4 5 6

7 8 9 10 11 12 13

14 15 16 17 18 19 20

21 22 23 24 25 26 27

28 29 30

SEPTEMBER 2014

Public Holidays (August 2014):

31st - National Independence Day (Merdeka Day)

Ground crystal of ARM lipase produced in the

optimized crystallization formulation that is 0.1

M Tris-HCl pH 8.0 and 12% (v/v) PEG 20000.

Top: 3D structure of ARM lipase (1.15 )


Space-grown crystals of ARM lipase obtained in a

gel-tube capillary using 0.1 M MES monohydrate pH

6.5 and 12% (v/v) PEG 20000 as precipitant, with

approximate dimension of 0.05 x 0.01 x 0.20 mm.


1 2 3 4 5 6

7 8 9 10 11 12 13

14 15 16 17 18 19 20

21 22 23 24 25 26 27

28 29 30

S M T W T F S

1 2

3 4 5 6 7 8 9

10 11 12 13 14 15 16

17 18 19 20 21 22 23

24 25 26 27 28 29 30

31

AUGUST 2014

S M T W T F S

1 2 3 4 5 6

7 8 9 10 11 12 13

14 15 16 17 18 19 20

21 22 23 24 25 26 27

28 29 30

OCTOBER 2014

Public Holidays (September 2014):

13th - Sarawak Governor's Birthday

16th - Malaysia Day

Enhanced elastase crystal size. Elastase crystal. (Top) Crystal

growth during crystal screening; (middle) crystal growth using

the hanging drop method; and (bottom) crystals in the capillary

using the microseeding counter-diffusion method.

SEPTEMBER 2014

Reference:

Mohd Shukuri Mohamad Ali, Zatty Syamimi @ Adura Mat Said, Raja Noor Zaliha Raja Abd Rahman, Adam Leow Thean Chor, Mahiran Basri, Abu Bakar Salleh (2013). Capillary-Seeding Crystallization and Preliminary Crystallographic Analysis of a Solvent-Tolerant Elastase from Pseudomonas aeruginosa Strain K. International Journal of Molecular Sciences, 14 (9), 17608-17617



1 2 3 4

5 6 7 8 9 10 11

12 13 14 15 16 17 18

19 20 21 22 23 24 25

26 27 28 29 30 31

OCTOBER 2014

Public Holidays (October 2014):

4th - Sabah Governors Birthday

5th - Hari Raya Haji

10th - Malacca Governor's Birthday

23rd - Deepavali (Diwali)

24th - Sultan of Pahangs Birthday

25th - Awal Muharram (Maal Hijrah)

S M T W T F S

1 2 3 4 5 6

7 8 9 10 11 12 13

14 15 16 17 18 19 20

21 22 23 24 25 26 27

28 29 30

SEPTEMBER 2014

S M T W T F S

1

2 3 4 5 6 7 8

9 10 11 12 13 14 15

16 17 18 19 20 21 22

23 24 25 26 27 28 29

30

NOVEMBER 2014

Left: L42 crystal in counter-diffusion method. Right: Diffraction pattern of L42 crystal in counter-diffusion (~2.0 ).

Left: L42 crystal in vapor-diffusion. Right: Diffraction pattern of L42 crystal in vapor-diffusion(~2.4 ).

Top: Solved 3D structure of L42 Lipase (1.4 )

Reference:

Mohd Saif Khusaini, Raja Noor Zaliha Raja Abd Rahman, Mohd Shukuri Mohd Ali, Adam Leow Thean Chor(2011). Preliminary X-ray Crystallographic Analysis of Thermostable Lipase 42. Acta Crystallographica section F 67, 401403



1

2 3 4 5 6 7 8

9 10 11 12 13 14 15

16 17 18 19 20 21 22

23 24 25 26 27 28 29

30

NOVEMBER 2014

S M T W T F S

1 2 3 4 5 6

7 8 9 10 11 12 13

14 15 16 17 18 19 20

21 22 23 24 25 26 27

28 29 30

OCTOBER 2014

S M T W T F S

1 2 3 4 5 6

7 8 9 10 11 12 13

14 15 16 17 18 19 20

21 22 23 24 25 26 27

28 29 30 31

DECEMBER 2014

Public Holidays (November 2014):

11th & 12th - Sultan of Kelantan's Birthday 22nd - Sultan of Johors Birthday 29th - Hari Hol Almarhum Sultan Iskandar Johor


Crystals of LipPI12, a cold active bifunctional lipase with protease activity from Antarctic microorganism Pi 12 grown in 0.1M HEPES sodium buffer at pH 7.5 with precipitants 20% w/v of PEG 4000 and 10% v/v of 2-propanol. The crystals take form in a shape of needle cluster.

Top: 3D structure of PI 12


1 2 3 4 5 6

7 8 9 10 11 12 13

14 15 16 17 18 19 20

21 22 23 24 25 26 27

28 29 30 31

DECEMBER 2014

S M T W T F S

1

2 3 4 5 6 7 8

9 10 11 12 13 14 15

16 17 18 19 20 21 22

23 24 25 26 27 28 29

30

NOVEMBER 2014

S M T W T F S

1 2 3

4 5 6 7 8 9 10

11 12 13 14 15 16 17

18 19 20 21 22 23 24

25 26 27 28 29 30 31

JANUARI 2015

Public Holidays (December 2014):

11th - Sultan Selangors Birthday 25th - Christmas


From left to right, from up to down:

The EMTECHs protein crystals: F16L/S157g lipase

(ground), F16L/S157g lipase (space), F16L lipase

(ground), F16L lipase (space), L2 lipase (ground), L2

lipase (space), L42 lipase (ground), PI12 lipase, S2F

lipase (ground), T1 lipase (ground), T1 lipase

(space), V171S, D311E lipase, ARM lipase (ground),

HZ lipase (space), HZ lipase (ground).

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