Working Plans & Goals Project year 2004

Metabolic profiling of Arabidopsis thaliana :: post-doc Jens Rohloff

AIMS OF RESEARCH – Model plant Arabidopsis thaliana

Characterize secondary metabolites in At with focus on the glucosinolate-myrosinase complex

Investigate T-DNA-knockout mutants being studied within The Plant Genetics Group at NTNU to sustain the group’s molecularbiological work

Study both abiotic and biotic factors affecting plant growth and metabolism regarding morphology and ontogenetic development

Metabolic profiling of Arabidopsis thaliana :: post-doc Jens Rohloff

The glucosinolate-myrosinase complex

Glucosinolates derived from amino acids- leucine- methionine- phenylalanine- tryptophane

Altered pathway of GLS induceschanges in biosynthesis of othersecondary metabolites involved in

- plant defense- plant-insect interactions- stress response

Detection of concentration levels of- Glucosinolates- Plant hormones- Amino acids- Volatiles, i.e. isothiocyanates, nitriles, terpenes, hydrocarbons

indole glucosinolates

Metabolic profiling of Arabidopsis thaliana :: post-doc Jens Rohloff

Preliminary Results 2003 - Volatiles from Arabidopsis

Metabolic Profiles fromA. Arabidopsis mutants (wt,TGG1,TGG2,Cyp83A1,Cyp83B1)

B. Ecotypes of Arabidopsis(Col, Cve, Ler, Was)

C. Plant organs(leaves, stems and flowers)

D. Living plants (in vivo) orAutolysed plants

Identified Volatiles Terpenes

Green leaf volatiles

Isothiocyanates + Nitriles

Hydrocarbons (alkanes, aldehydes ketones,esters)

Metabolic profiling of Arabidopsis thaliana :: post-doc Jens Rohloff

Gas chromatography analysis (GC-MS) ofvolatiles from Arabidopsis mutant Cyp83A1Method - headspace solid-phase microextraction

Z-3

-hex

eno

l

Z-3

-hex

enyl

ace

tate

4-m

ethy

l pen

tyl I

TC

2-et

hyl b

enzy

l IT

C

alph

a-ph

ella

ndre

ne

para

-cym

ene

hexy

l IT

C

hep

tyl I

TC

3-m

ethy

l but

yl I

TC

non

yl I

TC

1-he

xano

l

. . . over 100 chemical compounds have been tentatively identifiedby mass spectral database search (GC-MS) !

deca

nal

non

anal

tetr

adec

anal

Co-operation within BIOEMIT and outside the project

1. The Plant Genetics Group, Dep Biol, NTNU Ishita Ahuja (res.) + Anna Kusnierczyk (dr.stip.) plant-insect interactions

Anna Vera De Felice (post-doc) metabolomics

2. Institute of Physical Chemistry, Dep Chemistry, NTNU Prof. Bjørn Alsberg, Endre Anderssen (post-doc) bioinformatics

3. The Plant Biocentre, Dep Biol, NTNU Prof. Tor-Henning Iversen, Knut Robert Fossum (res.) space biology

4. Neuroscience Lab, MTFS, NTNU Stig Ulland (dr.stip.) plant-insect interactions

5. Group of Ecological Chemistry, Dep Org Chemistry, KTH Prof. Anna-Karin Borg Karlson biochemistry

Metabolic profiling of Arabidopsis thaliana :: post-doc Jens Rohloff

Metabolic profiling of Arabidopsis thaliana :: post-doc Jens Rohloff

First publication title (submission to Phytochemistry until April 2004)

“Altered headspace volatile profiles of Arabidopsis mutants with deficiencies in the glucosinolate biosynthetic pathway”

Conference15. Int Conf Arabidopsis Research, July 10 - 14, 2004, Berlin

Fellowship abroad (3 up to 6 months in 2005)Max-Planck-Institute at Jena or Potsdam, Germany>>> funding will be applied via EU (Marie Curie), NFR or DAAD