Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I...
Transcript of Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I...
![Page 1: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/1.jpg)
1
The world leader in serving science
Workflows in SIEVE and LipidSearch
I Orbi 5 - 2014
![Page 2: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/2.jpg)
2
The world leader in serving science
SIEVE v2.1
S tatistical
I terative
E xploratory
V isualization
E nvironment
![Page 3: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/3.jpg)
3
SIEVE Overview
• SIEVE is label-free differential software
• Aids in discovering molecular changes between states
• Provides semi-quantitative measurement of differentially expressed proteins, metabolites and other compounds correlating with a disease state, drug response or other perturbation
• Proteomics
• Biomarker discovery in plasma, tissue, cell culture, urine, saliva
• Disulfide bond validation in purified protein
• Quantify samples that contain their own precursor label,
similar to SILAC
• Small Molecules
• Biomarker discovery in plasma, tissue, cell culture, urine, saliva
• Monitoring drug degradation due to environmental stresses
• Metabolomic and Lipidomic profiling
• Ingredient screening in Food and Safety
• Water purification monitoring
• Improving agriculture (tomatoes, whiskey, wine, corn)
• Direct infusion of olive oil using DART, looking for impurities
• Screening purified therapeutics for environmental and/or product modifications
Scanning electron microsope image of a cancerous (left) and normal
cell, showing differences in cell “brush”. Image courtesy Igor Sokolov.
![Page 4: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/4.jpg)
4
Getting Started
• Minimal system requirements
• Microsoft Windows
• Windows 7 32/64 Professional SP1
• Windows XP 32 Professional SP1
• Microsoft .Net 4.0 (Extended)
• 2 GHz dual core processor
• 8 GB RAM or higher
• 500 GB hard drive
• Recommended system requirements
• 3.3 GHz processor
• 32 GB RAM
• 1TB RAID performance
• Installation requires administrator rights
• Free upgrade from v2.0 to v2.1 via the Thermo Omics Portal
• https://portal.thermo-brims.com/
• Not necessary to uninstall v2.0
![Page 5: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/5.jpg)
5
Statistically rigorous automated label-free LC/MS differential analysis platform
Applied to: any experiment that compares one group to another
State 1
Raw file
State 2
Raw file
State …
Raw file
Workflow
Align
Detect
Identify
Reports:
• Components
• Relative
Quantification
• Statistical Analysis
• Trend information
• Identification
SIEVE Analysis Platform
![Page 6: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/6.jpg)
6
Creating a SIEVE Experiment
Initiate the wizard
![Page 7: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/7.jpg)
7
Define Processing Method
Select the domain: proteomics or small molecules
![Page 8: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/8.jpg)
8
Define Processing Method and Experiment Type
Select the detection algorithm: which one?
![Page 9: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/9.jpg)
9
Two Signal Detection Algorithms – Which One to Use?
Classic Recursive Base Peak
Framing Component Extraction
Application •All sample types:
Proteomics
Small molecules
•Small molecules (charge state ≤2)
Instrumentation •High resolution instrument
•Low resolution instrument
•High resolution instrument
Experiment Type •Trend analysis
•Control Treated
•ROC analysis
•Single class analysis
•Trend analysis
Advanced
Processing
•Perfect pairs
•Targeted detection
•Direct infusion
•Background subtraction
•Data reduction by isotope and
adduct grouping
Limitations •NO background subtraction
•NO data reduction by isotope and
adduct grouping
•Requires charge state ≤2
•Requires high resolution data
![Page 10: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/10.jpg)
10
SIEVE Workflow – Component Extraction
[M+H]+
[M+Na]+
[M+K]+
Automatically interpret spectra, reduce signal peaks into components
A total of 9 different ions
are observed for the same
molecule as isotopes and
adducts
![Page 11: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/11.jpg)
11
Experiment Name
Identify experiment name
![Page 12: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/12.jpg)
12
Select Raw Files
Drag and drop the raw files from the file explorer
![Page 13: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/13.jpg)
13
Sort Raw Files
Click on the file name to sort the files
![Page 14: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/14.jpg)
14
Multiple Solvent Blanks
If more than one solvent blank is present then “blank” files are averaged
![Page 15: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/15.jpg)
15
~98% of lower intensity signals are eliminated
Sample - Solvent blank = Analyte signals Distinguish analyte signals
from noise
Background subtraction
is automatically
performed when
solvent blanks are
acquired.
Irrelevant solvent peaks
are removed from the
data that eliminates a
significant amount of
low level noise.
A significant step in
data reduction and a
critical part of the new
component detection
algorithm.
Component Extraction Background Subtraction
![Page 16: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/16.jpg)
16
Scan Raw Files for Data Quality
Raw files show no errors
![Page 17: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/17.jpg)
17
Define Analysis Groups
Identify group names by separating groups with a space
Select
alignment
reference
file
Ratio
group is
the control
group
(Fed/Fast)
The word
“blank” will
enable
background
subtraction
![Page 18: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/18.jpg)
18
Define Search Parameters
Define retention time, mass range, and m/z width
Frame
width is
“automatic”
for
component
extraction
m/z width:
10 ppm is
+/- 5 ppm
Define
frame width
for framing
experiment
![Page 19: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/19.jpg)
19
Select Scan Filter
SIEVE automatically selects the full MS scan type
Data with
both positive
and
negative
filters needs
to be
processed
separately
Used a lock mass?
Need to modify the filter string
to removing lock mass text
Example: FTMS + p ESI Full MS Lock Mass
![Page 20: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/20.jpg)
20
Intensity
threshold is
initially set
from the mean
intensity of the
reference file
Define Main Component Extraction Parameters
• Review raw data in Qual Browser first
• Each data set is different requiring different settings
![Page 21: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/21.jpg)
21
Define Identification Parameters
Three search types available: ChemSpider, Database Lookup or Defer
![Page 22: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/22.jpg)
22
• ChemSpider • Free chemical structure database
• Over 470 data sources i.e. KEGG, Human Metabolome Database, etc.
• More than one data source can be used in the identification search when separated by a comma
• DB Lookup • Post peak detection lookup (different from seed file)
• DB Library Files in csv format
• Requirement: first column must have neutral exact mass
• All other columns are optional
• Defer • Can defer this setting in the wizard
• Identification can be later enabled within the parameters table
Identification Parameters
![Page 23: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/23.jpg)
23
Complete Wizard Setup
Save file as .sdb
![Page 24: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/24.jpg)
24
Review SIEVE Parameters Before Processing
• Reference File
• Check if reference file is displayed
• If not, enable through raw file collection
• Scan Filter
• Exclude lock mass in text string
• Update
• If modifications made in the parameters table, UPDATE
• Then run processing task
• Align
• Always align
• Even if bypassing align step
• SIEVE is reading in files
• Multiple instances of software allowed
![Page 25: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/25.jpg)
25
Component Extraction - Use of Integration Parameters
• Peak Detection
• ICIS
• Genesis
• PPD (parameter-less)
• Peak Integration
• ICIS
• Genesis
• PPD
• None
• Peak areas generated
• Integration reflects entire
window
• Why use? Time
None
ICIS
Peak Integration
![Page 26: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/26.jpg)
26
Set Integration Parameters
Optimize
parameters for
chromatographic
peaks
![Page 27: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/27.jpg)
27
Unaligned Small Molecule Data
![Page 28: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/28.jpg)
28
Aligned Small Molecule Data
Zoom in by placing a box over area with cursor
Zoom out by removing scroll bars
![Page 29: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/29.jpg)
29
Frame Report View
![Page 30: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/30.jpg)
30
Data Review Options
XIC
Peaks
Trend
Intensities
![Page 31: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/31.jpg)
31
Frame Report
Right click on
any column
title to
access field
chooser
![Page 32: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/32.jpg)
32
Results Review Options
Gel
View
PCA
CVs
Displayed
by group
![Page 33: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/33.jpg)
33
Frames Table Filter
• Use filter table to reduce the number of components
• Filter on column headings
• Filter follows Boolean logic (and, or, not)
• Example 1: CV_E <20 and CV_H <20
• Example 2: Ratio_E < 0.5 or Ratio_E >1.5
• Example 3: Pvalue_E <0.05
• Example 4: Pick >0
![Page 34: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/34.jpg)
34
Additional Tips
• Each dataset may be different
• Visually confirm alignment (may need to bypass alignment)
• Multiple iterations of peak detection may be necessary to optimize peak detection parameters
• Start with higher threshold and no peak integration for faster review
• Supplemental information provided
• Questions? Refer to the Thermo Omics Software Portal
• http://portal.thermo-brims.com/
![Page 35: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/35.jpg)
35
The world leader in serving science
Lipid Search v 4.0.20 Quick Fix release
![Page 36: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/36.jpg)
36
LipidSearch Features
• Automated identification of lipids from biological samples
• Identification, relative quantitation, alignment
• Comprehensive database of >1.5 million lipid ions and predicted fragment ions
• Identification algorithms for product ion, precursor ion, and neutral loss scans
• Identification ranked by mass tolerance, then matched to predicted fragments and predicted retention time
• Suitable for multiple approaches for lipid analysis
• LC or nano-infusion (Shotgun)
• Untargeted and targeted profiling
• Compatible with data from various MS systems
• Thermo Q Exactive, hybrid Orbitrap, and TSQ instruments
![Page 37: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/37.jpg)
37
Getting Started
• Recommended system requirements
• 64-bit operating system, Microsoft Windows 7/8
• Quad- or multi-core CPU, 3 GHz or higher
• 16 GB RAM or higher
• 500 GB hard drive or larger (SSD optional)
• Required programs
• Thermo Scientific MSFileReader 64-bit (need to uninstall if currently installed)
• Java runtime environment (JRE 1.6+)
• Microsoft Visual C 2010 runtime
• Microsoft Internet Explorer or Google Chrome
• Web-based graphical interface
• Installation requires administrator rights
![Page 38: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/38.jpg)
38
Getting Started
• Tomcat Server
• Adjustable maximum memory allocated to server
• Installation
• Edit after installation
• Documentation
• User manual, installation instructions, tutorial files (C drive)
C:\lipidserach\lipidserach4.0\LipidSearchLauncher\LipidSearchLauncher.ini
![Page 39: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/39.jpg)
39
Launcher
• Initiate the software via the desktop icon
• Tomcat server
• Open to launch LipidSearch
• Minimize Tomcat server to the taskbar
• Re-open server by clicking on icon
• http://localhost:8090/lipidsearch040/
Stop and start
server here
![Page 40: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/40.jpg)
40
• Must request license key to register software
• Send information to ThermoMSLicensing.com
• Register key to activate software
License
![Page 41: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/41.jpg)
41
Configuration
• Modify configuration to improve performance
• Increase buffer size to 70 – 80%
• If using > 3 GHz processor, increase the number of processes for
peak detection, identification, and quantification to 4
![Page 42: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/42.jpg)
42
Step 1
Step 2
LipidSearch Workflow
![Page 43: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/43.jpg)
43
Batch Creation for Identification and Quantitation
Select raw
files to be
processed
![Page 44: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/44.jpg)
44
Identification Parameters - LC-MS/MS
Recalc Isotope:
ON for general search
OFF for low abundant ions
M-Score is based on the number of matches with
product ion peaks in the spectrum
General:
Triple Quadrupole
Q Exactive:
QE or Fusion (HCD)
Orbitrap: Fusion
(CID, MS2/MS3)
![Page 45: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/45.jpg)
45
Quantitation Parameters
![Page 46: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/46.jpg)
46
Filter Criteria for Displaying Raw File Results
4) ID Quality:
A: lipid class & FA were completely identified
B: lipid class & some FA were identified
C: lipid class or FA were identified
D: identification by other fragment ions (H20 loss)
1) Toprank:
displays lipids with
top score among
identified spectra
2) Main node:
main isomer peak
displays the largest
isomer based on
intensity, m-score
and t-score
3) FA priority:
shows the most likely fatty
acid chain combination if
lipid isomers have the
same score
1 2
3 4
![Page 47: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/47.jpg)
47
Select Lipid Class
![Page 48: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/48.jpg)
48
Select Adducts
![Page 49: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/49.jpg)
49
Submit Batch
Successful submission
Unsuccessful submission
![Page 50: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/50.jpg)
50
Data Processing – Search Job List Window
Export: Exacts the summary data in the results list
Download: Exacts the entire results file
![Page 51: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/51.jpg)
51
Data Processing – Search Job List Window
Number of lipid groups
Number of lipid ions
Identification parameters window
Identification results window
![Page 52: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/52.jpg)
52
Identification Results Summary
• The parameters applied
in the filter can be
modified then resubmitted
with the change filter
function
•This operation can be
performed in the job list
window or the in the
identification results
window
![Page 53: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/53.jpg)
53
Data Processing – Search Job List Window
P Peak picking
I Identification Q Quantitation
![Page 54: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/54.jpg)
54
Data Processing – Search Job List Window
In queue
Active
Canceled
Successful completion
Ended in failure
![Page 55: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/55.jpg)
55
Data Processing – Search Job List Window
Number of lipid groups (sum composition)
Number of lipid ions (isomers)
![Page 56: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/56.jpg)
56
Review Identification Results
![Page 57: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/57.jpg)
57
Review Identification Results
Data sorted by LipidGroup, CalcMz, TopPos
![Page 58: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/58.jpg)
58
Review Identification Results
•t-score: the difference between the theoretical LC-MS retention time (RP) calculated from the lipid
computational formula and the actual retention time [lower value increases reliability]
•m-score: based on the number of matches with product ion peaks in the MS2 spectrum [higher value]
•Occupancy rate: the ratio of MS2 spectrum peaks assigned to the lipid among all peaks [higher value]
•Grade: identification quality filter assigned A – D based on lipid class or fatty acid identification
![Page 59: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/59.jpg)
59
Review Identification Results
Chromatographic peak Mass spectrum
![Page 60: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/60.jpg)
60
Spectrum Details Screen – Data ID
Black =
unassigned ions
Red = MS2
matched ions
Green = MS3
matched ions
Precursor ion
Other spectra
where this lipid
is identified
![Page 61: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/61.jpg)
61
Chromatogram Chart
Area score: 0.96
De-noising Smoothing Separating partially
overlapped peaks
Yellow = integrated area
![Page 62: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/62.jpg)
62
Alignment Parameters
Set
alignment
parameters
Max peak area is
the default
Select Mean to
obtain group
average peak areas
![Page 63: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/63.jpg)
63
Retention Time Tolerance
LC Experiment Types: Retention time tolerance
threshold for the peak tops of peaks deemed to be the
same lipid during alignment
r.t.1 – r.t.2 > R.T. tolerance
If the above is true then the peaks will not be aligned
Instead they are two separate records in the results list
Example results: LPC (18:1)
![Page 64: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/64.jpg)
64
Select Raw Files for Alignment
Click select to
open the job list
tab
![Page 65: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/65.jpg)
65
Select Raw Files for Alignment
Check box to include raw files to be aligned
Click
add
![Page 66: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/66.jpg)
66
Group Allocation
•Selected files will appear
in alignment setup
•Define control and
sample groups
•In this example, wild type
is control and knock out is
sample group 1
![Page 67: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/67.jpg)
67
Submit Alignment
Successful submission
![Page 68: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/68.jpg)
68
Alignment Jobs List
M Merge (Alignment)
![Page 69: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/69.jpg)
69
Alignment Results
Group information
can be expanded
and collapsed
Alignment layout is formatted similarly to the identification results
![Page 70: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/70.jpg)
70
Normalization Option
Normalization by either internal standard or by individual lipid class
![Page 71: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/71.jpg)
71
Lipid Specific Alignment Results
![Page 72: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/72.jpg)
72
Acknowledgements
• Thermo Fisher Scientific
• Jennifer Sutton
• David Peake
• Ralf Tautenhahn
• Josef Ruzicka
![Page 73: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/73.jpg)
73
The world leader in serving science
Supplemental Material for SIEVE
![Page 74: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/74.jpg)
74
Define Experiment Type
Experiment Types: •Two Sample Differential Analysis – A simple comparison between two states such as healthy and diseased. A ratio and p-value are calculated. •Control Compare Trend – This experiment is used for time course analysis or trend type experiments. One of the groups is defined as the control group and the others are compared to this control group. For each trend point, a ratio and p-value are calculated against a control group. •Differential Case Study with ROC Analysis – This experiment type is used to measure candidate marker’s capability of distinguishing between two classes. A large subject group (≥10) is recommended. Technical replicates are also recommended. •Non-differential Single Class Analysis – Allows for a quick assessment of the data to determine reproducibility and overall quality by using the CV processor. This analysis can also be used with SIEVE’s Perfect Pairs tool to find precursor pairs in a single raw file. This algorithm tags pairs of frames that are consistent with a designated mass difference. Applications include PTMs, ion and ion + adduct combinations, SILAC, and other precursor labeling methods.
![Page 75: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/75.jpg)
75
Parameter Settings – Global
Change to force calculation if you want to see PCA plot for
large experiments.
Rawfile collection is where you can add/remove RAWs,
change the alignment reference file, change groups, color.
Scanfilter is the Full scan type that is used for the
analysis. If you have lock mass turned on and you see
two full scans in the wizard you need to change this
setting before running the analysis to include all of the full
scans. Change it so that the string includes FTMS + p
ESI Full, in this example. Remove all of the letters after
the Full
Bold line parameters are
the one that are changed
most often
Maximum number of threads for processing. Lowering this
value on 32 bit computers can bypass memory issues.
Retention start and stop can be used to eliminate un
wanted data.
After completely the wizard, the user must check to see if
the reference file parameter is populated. If a file is not
assigned the user must click on the rawfiles (collection)
tab and check the reference file in the box that appears,
Then select OK and the reference file parameter should
display the selected reference file.
![Page 76: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/76.jpg)
76
Parameter Settings – Alignment
Check alignment to see if alignment is needed or not. You
have the option to bypass alignment if needed.
Minimum intensity threshold for alignment
Bold line parameters are the one that are changed most often
Alignment correlation bin size
Max retention time shift for alignment step in mins
The initial size of a title that correlates basepeak
alignment.
![Page 77: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/77.jpg)
77
Signal to background noise threshold for
background correction (subtraction).
Suppress components that do not meet the
Background Signal to Noise criteria.
Base peak minimum intensity required for a
signal to be considered as a component.
Minimum number of scans across a
chromatographic peak to be considered.
Mass window for XIC in ppm (NOTE: 10ppm
= +/- 5 ppm, not +/-10ppm).
Algorithm used to determine peaks ICIS
because the parameters can be checked and
optimized by looking at the raw data in Qual
Browser.
Time in mins from a peak apex to restrict
seeking another peak.
Base peak minimum intensity requires for a
signal to be considered as a component from
a targetMZlist experiment.
List of component MZ’s to force find.
Bold line parameters are the one that are changed most often
Parameter Settings – Basic Component
![Page 78: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/78.jpg)
78
The condition or trend point that was
designated as the control. The control group
serves as the denominator for ratio
calculations (treatment/control).
Algorithm used for second pass peak
integration. Default is NONE but if ICIS is
used for peak detection the ICIS should be
used for peak integration. Parameters for
the different integration methods can be
found under the Workspace tab at the top of
the software. It is recommend that the user
look at their data in QualBroswer first and
optimize the peak integration parameters
(ICIS) and then apply these settings to
SIEVE. These parameters are very sensitive
to the chromatographic peak shape of the
data.
Bold line parameters are the one that are changed most often
Parameter Settings – Frame
![Page 79: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/79.jpg)
79
Skirt minimum basepeak intensity, default
should be set to 5 million but varies from
instrument to into. 5 million for QE and 2
million for Exactive.
Number of points for data smoothing.
Bold line parameters are the one that are changed most often
Parameter Settings – Advanced Component
Minimum scans should always remain 2, do
not change this setting it is not base peak
minimum scans.
![Page 80: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/80.jpg)
80
The charge used for ChemSpider and
DBLookup if a charge could not be
determined.
The maximum number of
frames/components to identify.
Multiple formulas may be assigned to each
component. MinFormulaScore is the
minimum number of formulas sent to
ChemSpider for identification or sent for
pathway analysis. Maximum number of
formulas report per component is 10.
Select the search type: ChemSpider,
DBLookup, None
Bold line parameters are the one that are changed most often
Parameter Settings – Global Identification
![Page 81: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/81.jpg)
81
ChemSpider identification only: Provide
adduct for mass calculation (+nH, -nH +K
+Na +NH4
ChemSpider databases used for search.
More than one database can be search at a
time and should be separated by commas.
Browse for the accurate mass library file
(csv).
Use either COMPMW to find accurate mass
ID’s based upon component molecular
weight, FRAMEMZ to find accurate mass ID’s
based upon frame MZ, or FORMUAL to id by
formula. For COMPMW search type adduct is
not necessary.
Accurate mass MZ tolerance for DBLookup
and ChemSpider searches (ppm).
Bold line parameters are the one that are changed most often
Parameter Settings – Accurate Mass Identification
![Page 82: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/82.jpg)
82
Time in mins from a peak apex to restrict seeking
another peak. Default setting is 0.2 which is too
large to separate isomers.
Max retention time shift for alignment step in
mins. Default setting is 0.2 which is too large in
this case to separate isomers.
Peak Integration is changed from NONE to ICIS.
Smoothing set to Points 3
Parameter Settings – Optimized
BPMinimumCounts changed to 1 million
Background SN changed to 10
SkirtBPInty set to 5 million
![Page 83: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/83.jpg)
83
Frame Target Wizard – Seed File
•Incorporates a search for target ions of interest
•Identify .csv file in the setup wizard
•Alternatively identify .csv file in the parameters table as frame seed file
![Page 84: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/84.jpg)
84
Frame Target Wizard
•Create the .csv file
•Minimally required columns include MZ, RTStart, and RTStop
•Additional information can be listed in columns, for example compound name
•The annotated column can be filtered using the frames table filter
![Page 85: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/85.jpg)
85
Frame Target Wizard
•Assign columns within the .csv file
•Check the number of entries in the frame parameters table to confirm file is successfully
identified
![Page 86: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/86.jpg)
86
Normalization
• If normalizing to a selected frame, make sure the desired m/z frame is highlighted
• The desired frame is then displayed as the frame to normalize to in the normalize tab
![Page 87: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/87.jpg)
87
Alignment and Framing Experiment
•Associate data by isotopic cluster
•Select PRElement, PRRoot, and PRSize from the field chooser
•Sort by PRRoot
•Tip: filter on PRSize
PRElement
0 = 12C
1 = 13C
2 = 14C
PRRoot 12C peak
PRSize
number of
frames per
cluster
![Page 88: Workflows in SIEVE and LipidSearch - Thermo Omics Portal · Workflows in SIEVE and LipidSearch I Orbi 5 ... Reports: • Components ... Experiment Type •Trend analysis •Control](https://reader031.fdocuments.us/reader031/viewer/2022020204/5aed802e7f8b9a3b2e90acf5/html5/thumbnails/88.jpg)
88
Elemental Composition
• SIEVE can generate
up to 10 possible
elemental compositions
for a given component
•Each composition is
scored and ranked
•The top ranked
composition is listed in
the main frame report
table
•Other possible
compositions can be
viewed in the Flex View
tab