Workflow Diagram for the Natural Product/Metabolite/Discovery Chemist

LC

vacuumcentrifuge

MSDAD/ELSD

orbioactivity

NMR

Purification FractionCollection

orPurifiedSamples

Prep Stage

Report

Crude Samples/Mixtures

Low Sensitivity Detection

High Sensitivity Detection

1

Modern Sample Formats:The Wellplate and the Microvial

•No shimming between samples•No washing tubes

•Minimal sample transfer

•Efficient storage

•Disposable

•Economical

Incredible Time Savings

Plates Make It Easy2

Protasis One Minute NMR Automation

SampleReturn

StackedSample

Drawers

WashBays

MicroliterPrecision

Near ZeroDead Volume

EasyParameter

Setup

SolventConditioning

Wellplate

Web-basedSoftware

3

Solvent

Microfilter

VialSpatula

Microfilter Sample

Step 1: Add sample (5 – 500 g)

Step 2:Add solvent (5 – 15 L)

Step 3: Spin down(~ 1 min.)

Step 4: Discard microfilter

Step 5: Place vial on OMNMR

Quality Results Start with a Quality Sample

4

Preparative Filtration, Sample Concentrator

Microfilter

Vial

Protasis P/N: PREP-OAFST-FV• Unique Protasis product• Ensures particulate-free,

high-quality sample• Benchtop “Point of Use”

Centrifuge• Ideal for open-access• 500 Microfiltration Vials• Easy to use!

Discrete Sample MicrofiltrationVacuum Centrifugation

Protasis P/N: PREP-OAFST-VC• Protonated-to-deuterated solvent• Sample concentrator• Variety of vendor options• Customer may opt to purchase

separately

Summary:Open-access, discrete sample filtration? PREP-OAFST-FVSolvent removal, deuterated solvent replacement? PREP-OAFST-VC