WORK UP LABORATORY AND IMAGING - Medical...
Transcript of WORK UP LABORATORY AND IMAGING - Medical...
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WORK UP
LABORATORY AND IMAGING
Dr. Rodney Martinez 1
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IMAGES AND LABORATORY WORK UP
1. Indications and Purposes
2. Pre-test Preparation
3. Post-test Management
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WHY DO WE USE ADDITIONAL TEST?
Clinical history
Physical examination
Complementary exams (Work Up)
Laboratory medicine
Imaging
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FACTORS AFFECTING MEASURABLE VARIABLES
• Age.
• Sex.
• Ethnicity.
• Altitude.
• Build.
• Physiological conditions (e.g. at rest, after exercise, standing, lying).
• Sampling methods (e.g. with or without using tourniquet).
• Storage and age of sample.
• Container used, e.g. for blood sample, as well as anticoagulant.
• Method of analysis
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SENSITIVITY & SPECIFICITY
Sensitivity % of patients with the disease and in
whom the test is positive
Specificity % of people without the disease in whom
the test is negative
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LABORATORY 6
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ELECTROLYTES
SODIUM
-bsorbed from the small intestine and excreted in the urine in amounts dependents dependent on dietary intake
-ustains osmotic pressure and acid base balance
-s major extracellular cation
-ormal daily requirement is 15 mEq
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ELECTROLYTES
POTASSIUM
-romote cellular water balance, electrical conduction in muscle cells, and acid base balance
-btains K through dietary ingestion and the kidneys preserve or excrete K
-o evaluate cardiac, renal, and gastrointestinal function
- major intracellular cation
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ELECTROLYTES
CHLORIDE
-ighly abundant body anion in the extracellular fluid
-ounterbalance cations and buffer
-ets digestion and maintenance of osmotic pressure and water balance
H
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ELECTROLYTES
MAGNESIUM
lotting mechanism ontrols neuromuscular
activity ofactor that modifies activity
of many enzymes alcium metabolism
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ELECTROLYTES
CALCIUM
-one formation -n conversion of
prothrombin to thrombin -ransmission of nerve
impulse -n contraction ok skeletal
and myocardial muscles
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COAGULATION STUDIES
ACTIVATED PARTIAL THROMBOPLASTIN
TIME (APTT)
-mount of time it takes in seconds for
recalcified plasma to clot after partial
thromboplastin is added
-erformed for patient receiving heparin
-est for deficiencies and inhibitors of clotting
factors
-ime: 20 to 36 seconds
A
P T
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COAGULATION STUDIES PROTHROMBIN TIME (PT) and
INTERNATIONAL NORMALIZED RATIO (INR)
-rothrombin is a vitamin K dependent glycoprotein produced by the liver for fibrin clot formation
-o monitor response to warfarin sodium (Coumadin)
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COAGULATION STUDIES PROTHROMBIN TIME (PT) and
INTERNATIONAL NORMALIZED RATIO
(INR)
Normal Values:
PT:
9.6 to 11.8 secs (male)
9.5 to 11.3 secs (female)
INR:
2.0 to 3.0 (standard warfarin tx)
3.0 to 4.5 (high dose warfarin tx)
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COAGULATION STUDIES CLOTTING TIME
-lient should not receive heparin 3 hours before specimen collection
-ong on any anticoagulation therapy
-n thrombocytopenia
-ime: 8 to 15 minutes
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COAGULATION STUDIES PLATELET COUNT
Plug formation
Clot retraction
Coagulation factor
activation
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COAGULATION STUDIES PLATELET COUNT 150T – 400T
cells/mm3
<PLT – thrombocytopenia (risk for bleeding)
>PLT – thrombocytosis (risk for clot) – prophylaxis of Anicoagulant - Lovenox
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FULL BLOOD COUNT (FBC)
• Called complete blood count (CBC) in the USA.
• Red series
• Total red blood cells (absolute number per liter)
• Hemoglobin concentration (grams per deciliter)
• Hematocrit (Pack Volume Concentration) %
• Red blood indices
• Mean Corpuscular Volume (MCV) (fembtolitres)
• Mean Corpuscular hemoglobin (MCH) (picograms)
• Mean Corpuscular Hemoglobin Concentration (MCHC )
• Red blood cells distribution (RDW)
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ERYTHROCYTE STUDIES
ERYTHROCYTE SEDIMENTATION RATE
(ESR)- 0 to 30 mm/hr
ndirectly measures how much
inflammation is in the body.
pecial preparations not needed, but fatty
meal may cause plasma alterations
ate at which erythrocytes settle out of
anticoagulated blood in 1 hour
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ERYTHROCYTE STUDIES
RED BLOOD CELLS
-esults in the delivery of oxygen to the body
tissues
-lood diseases diagnosis
-irculate for 120 days and are removed from
the blood via the liver, spleen, and bone
marrow
-pecial preparation not needed
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ERYTHROCYTE STUDIES
RED BLOOD CELLS 4.5-5.5 million/mm3
<RBC – Anemia (Faitgue, SOB)
>RBC – Polycythemia (erythrocytosis) – management phlebotomy
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ERYTHROCYTE STUDIES
HEMOGLOBIN and HEMATOCRIT
Hemoglobin is the main component of
erythrocytes and serves as the vehicle
for transporting O2 and CO2
Normal Values:
14 to 16.5 g/dl (male)
12 to 15 g/dl (female)
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ERYTHROCYTE STUDIES
HEMOGLOBIN and HEMATOCRIT
Hematocrit represents red blood cell mass
and is an important measurement in the
identification of anemia or polycythemia
Normal Values:
42% to 52% (male)
35% to 47% (female)
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WHITE BLOOD CELL COUNT
WHITE BLOOD CELL
Immune defense system of the body
WBC 5,000-10,000 cells/mm3 <WBC – leukopenia (risk for infection)
>WBC – leukocytosis (infection/inflammation)
>100,000 – incapable of phagocytosis (leukemia)
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CARDIOLOGY
Cardiac enzimes
LDH
CK
TROPONIN
OTHERS
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CARDIAC MARKERS
CREATINE KINASE (CK)
Found in:
CK-MB (Cardiac)--- 0% to 5%
CK-BB (Brain)--- 0%
CK-MM (Muscles)--- 95% to 100%
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CARDIAC MARKERS
CREATINE KINASE (CK)
R: 6 hours
P: 18 hours
N: 2 to 3 days
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CARDIAC MARKERS
LACTASE DEHYDROGENASE (LDH)
R: 24 hours
P: 48 to 72 hours
N: 7 to 14 days
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CARDIAC MARKERS
TROPONIN
- and I
-egulatory protein found in striated muscle
-n bloodstream when an infarction causes damage to the myocardium
T
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CARDIAC MARKERS
TROPONIN I
>1.5 ng/ml… MI
R: 3 hours
N: 7 to 10 days
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CARDIAC MARKERS
TROPONIN T
>0.1 to 0.2 ng/ml… MI
R: 3 hours
N: 7 to 14 days
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CARDIAC MARKERS
MYOGLOBIN
Oxygen-binding protein
found in striated muscle
that releases oxygen at
very low tensions
Injury to skeletal muscle will
cause a release of
myoglobin into the blood
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CARDIAC MARKERS
MYOGLOBIN
>90 mcg/L… MI
R: 1 to 2 hours
P: 4 to 6 hours
N: 24 to 36 hours
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SERUM LIPIDS
Total Cholesterol---
140 to 199 mg/dl
Low Density Lipoprotein (LDL)--- <130
mg/dl
High Density Lipoprotein (HDL)--- 30 to
70 mg/dl
Triglycerides---
< 200 mg/dl
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URINE DIPSTICK TESTING
• Depending on the type of dipstick used, urine
can be tested for
• pH.
• Specific gravity.
• Haemoglobin.
• Leucocyte esterases and nitrites.
• Glucose.
• Ketones.
• Protein.
• Urobilinogen.
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POISONING & OVERDOSE
• Amphetamines & derivatives (MDMA (ecstasy),
MDEA (eve), MDA (adam)
• Anticonvulsants
• Benzodiazepines
• Carbon monoxide
• Cocaine
• Cyanide
• Digoxin
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ENDOCRINE INVESTIGATION
Fasting plasma
glucose
A1C
GH
TSH, T3 & T4
Norepinephrine and
epinephrine
Cortisol testing
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RENAL MEDICINE
Serum creatinine
Serum urea
24hrs creatinine clearence
Anion gap
Urine culture
Urine microscopy
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RHEUMATOLOGY
Rheumatoid factor
Antinuclear antibody (ANA)
Anticentromere antibodies
Anti-DNA
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IMAGING 40
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CHEST X-RAY
A-natomy
A-ppearance 41
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CHEST X-RAY PREPROCEDURE:
emove all jewelry and other metal objects from the chest area
ssess the client’s ability to inhale and hold his or her breath
ou question women regarding pregnancy or possibility of pregnancy
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CHEST X-RAY
POSTPROCEDURE:
Control the technique
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ULTRASOUND
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USES
Main imaging study in OBGYN
Study blood vessels like
Vena cava
Aorta
Study solid organs like
Heart
Liver
Gallbladder
Kidneys
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PROPERTIES
Advantages
Unexpensive
Shows the organs and structures around it
No side effects
Disadvantages
It cannot penetrate bone structures
Is operator deppendent
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CT SCAN
Scans the following in
successive layers by a
narrow beam of x-rays:
ngiogram
elly and Pelvic
hest
’ heart
xtremities
A
B
C
D
E
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CT SCAN
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CT SCAN PRETEST:
ssess allergies to iodine and seafoods
e sure to obtain informed consent
onscious sedation for claustrophobia
o remove jewelries and hair pins
xplain hot flushed sensation and metallic taste in
the mouth when dye is injected
luids and hydration
ive instruction to lie supine with small pillow under
the head
old if pregnant
t takes 20 minutes
A
B
C
D
E
F
G
H
I 49
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CT SCAN
POSTTEST:
llergic reaction check
e sure to replace fluid
VS
istal pulse check
xtremity color check
ind bleeding and hematoma
A
B
C
D
E
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MRI
-RI is nonivasive
-eveals types of tissue, tumors and vscular abnormalities
-s similar to CT scan
M
R
I
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MRI
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MRI PRETEST
-etal objects must be removed
-ssess for ineligibility and contraindications
-ive instruction to lie supine with small pillow under the head
-ormal audible humming, thumbing, grating, or knocking sounds
-ncourage conscious sedation for claustrophobia
-akes 45 to 60 minutes
-nformed consent
-ompletely enclosed in scanner
M A
G
N
E
T
I
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MRI POSTTEST
Resume normal activities
Fluids and hydration
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MRI Ineligible to undergo MRI: Automatic Internal Defibrillator
Cerebral Aneurysm Clip
Cochlear Implant
Hip Replacement
Knee Replacement
Non-removable dental prosthesis
Pacemaker
Prosthetic Valve Replacement
Soldiers
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EEG
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EEG • graphic recording of electrical
activity of the brain by several small electrodes placed on the scalp
To diagnose:
bnormal firing of electrical activity
rain tumors
ertain psychiatric disorders
egenerative disorders
nflammation of brain and spinal cord
A
B
C
D
E
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EEG PRETEST
ash the client’s hair
ssure that electrodes will not cause electric shock
timulants and depressants avoided for 24 to 48 hours
ypoglycemia prevention, do not omit breastfeeding
W
A
S
H
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EEG POSTTEST
Wash the client’s hair
Maintain side rails and
safety precaution, if the
client was sedated
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EEG
Sleep Deprivation EEG 60
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SKULL X-RAY
Radiographs of the skull:
ize
hape
uture separation
ome calcification
hows erosion and fracture
S
S
S
S
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SKULL X-RAY
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SPINAL X-RAY
Spinal radiographs:
bnormal spine and dislocation
one degeneration
ompression
eformed curvature
rosion
racture
A
B
C
D
E
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SPINAL X-RAY
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SKULL AND SPINAL X-RAY
-clude metal items from
body parts
-eassure nursing support
-ccurate documentation if
with thick and heavy
hair
-ou immobilize
X
R
A
Y 65