Winterhardiness Genetics for the Northern Rockies In 2003, Charlie Rife from KSU sent a collection...

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Winterhardiness Genetics for the Northern Rockies In 2003, Charlie Rife from KSU sent a collection of Russian winter canola lines that he hoped was the ‘Holy Grail’ for winterhardiness to test in Montana against his most winterhardy K-State genetics. This rainfed trial was sown directly into standing wheat stubble using low-disturbance disk openers. Fall rain arrived late so seedlings were only at the 1 to 2-lf stage at dormancy. Overwinter survival was very low (Fig. 2). Bad News: The Russian germplasm collection was not the ‘Holy Grail’. Good News: Winterhardiness of K-State genetics was likely the best in the world. Importance of Fall Irrigation In the 2004 trial it was obvious that we were going to lose yet another rainfed trial due to insufficient fall rain. We decided to rescue the trial with a single application of sprinkler irrigation in mid-Sep 2003 (75 mm = 3 inch). The K-State genotypes showed superior winterhardiness compared with the European checks (data not shown) and the yield of the 7 highest entries averaged > 2500 kg ha In 2005 we tested the importance of fall irrigation at Bozeman. Significant rain (1.4 inch) fell in September leading to timely emergence and fall growth, and thus, only minor differences among water regimes (Fig 4.). Strategies for High Yielding Winter Canola in the Northern Rocky Mountain Strategies for High Yielding Winter Canola in the Northern Rocky Mountain Region Region Perry Miller Perry Miller 1 1 and Duane Johnson and Duane Johnson 2 2 Rationale A successful bio-oil industry will depend on feedstock oil crops with a low cost per unit of production. Winter canola has high yield potential due to synchrony of plant growth with the environment of the northern Rockies. Preliminary research has shown that soil water in early September is a key constraint to winter canola survival. The advent of RR winter canola removes a major source of agronomic risk related to weed management. Objective To test the relative importance of 1) fall irrigation, 2) fall seeding date, 3) plant density, and 4) cultivar, for winter canola survival, seed yield, and oil production. Methods 1)Bozeman (southwest MT) and Kalispell (northwest MT) represent mild winter climates in the Rocky Mountain Front region of Montana. 1971-2000 average annual precipitation was 421 and 516 mm, respectively. Bozeman has a deep silt loam soil and Kalispell has a deep sandy loam soil. 2)Preliminary research has been underway since 2000 at Bozeman and 2002 at Kalispell. It has included cultivar evaluation and agronomic factors such as fall irrigation and seed density. 3)The current 2007 sites were established in Sep 2006 under line-source sprinkler irrigation gradients at both locations. Agronomic factors in the experimental design are: i.Seeding date (early vs late September) ii.Cultivar (European RR vs winter hardy non-RR KS line) iii.Seeding rate (25, 50, 100, 200, and 400 seeds m -2 ) We will collect data on emergence rates, plant development rates, crop yield, and oil yield. Preliminary Research During 2000-2003 at Bozeman, winter canola cultivar evaluation trials were seeded using low disturbance direct-seeding methods in dense cereal stubble. Our goal was to test the adaptation of winter canola in rainfed no-till systems managed for optimal snow cover. Due to dry fall conditions, the stands emerged late each year and small desiccated seedlings entered the winter in the 0 to 2-lf stage, strongly limiting winter survival. In some years green seedlings were present for 24-48 hr after snowmelt, then quickly rotted. We concluded that winter canola was not a viable option for rainfed no-till cropping systems because seedling establishment was not timely during drought-prone fall environments. 1 Dept. Land Resources and Environmental Sciences, Montana State University, Bozeman, MT; 2 Dept. Research Centers, Montana State University, Kalispell, MT We gratefully acknowledge support from the Montana Ag Experiment Station, the Pacific Northwest Canola Research Program, and the MSU Bio-Based Institute. Collaboration with Charlie Rife at Kansas State Univ. was invaluable. Technical expertise was ably provided by Jeff Holmes, Louise Strang, and 0 2 4 6 8 10 12 14 KS2004 R W N 215 R W N 224 KS983565a KS986661a R W N 222 KS2002 R W N 204 R W N 201 KS3579 KS983561a KS983567a Abilene Wichita R W N 205 R W N 213 R W N 225 R W N 210 R W N 207 R W N 227 R W N 206 R W N 216 KS983564a R W N 212 R W N 221 R W N 219 R W N 226 KS1701 R W N 211 R W N 214 R W N 228 Plainsman R W N 229 R W N 202 plants/m2 Figure 2. Winter canola stand densities measured 14 Apr 2003 at Bozeman, MT. Purple bars represent genetic lines from K-State and red lines represent genetic lines from Russia. European Yield Potential An unexpected result of the 2005 cultivar evaluation trials in Montana was that the European checks posted greater yields than the K-State genetic lines (Fig. 5). Excellent winter survival occurred at both locations. Managing Plant Density for Winterhardiness The 2005 trial at Bozeman showed that the greatest yield came from the lowest plant density (80 plants m -2 ; Fig. 4). Measurements of seedling biomass in the fall showed that total seedling biomass increased with plant density but that individual plant weight decreased with plant density (Fig. 6). Understanding this relationship may be critical to managing winter canola for optimal winter survival and oil yield in the northern Rockies. 0 10 20 30 40 50 60 IN T-E X 0386-R R IN T-E X 0354-R R IN T-E X 0362-R R KS2004-M T RW N 215-M T KS3019 K S 9866-6-1A -M T KS3021 KS3007 R W N 215 KS3020 KS2002 KS2004 KS3022 Kronos B aldur KS3017 KS2002-M T KS3011 W ichita KS3003 KS3018 K S 9835-6-5A -M T bu/ac (10% mois Figure 3. Seed yield of winter canola at Bozeman, MT, 2004. The 7 entries with dark blue bars were in the highest statistical group. The 3 lowest yielding entries with red bars were from Germany and had limited survival but thin stands were harvested due to weed management with the RR trait. The pink bars were also European entries. LSD (0.05) was 9 bu/ac. 0 5 10 15 20 25 30 35 40 45 Fallw ater leveland spring plantdensity b u /a c (1 0 % m Figure 4. Seed yields in the Fall Irrigation x Rate of Seeding trial, at Bozeman, MT, 2005. The genetic line KS9835-6-5A-MT was used for this trial. 0 5 10 15 20 25 30 35 40 45 Banjo K ronos K S 3018 W ichita K S 3007 KS9835-6-5A-M T K S 3011 K S 3020 K S 3003 K S 3021 KS9866-6-1A-M T K S 3017 RW N215-M T K S 2002 K S 2004 b u /ac (10% mo 10 20 30 40 50 60 70 80 90 100 110 Kronos N PZ 0326 AR C 92007-2 KS9135 Titan AR C 2189-1 AR C 2180-1 KS3018 Baldur AR C 92004-1 KS9124 Baros Wichita Jetton Abilene VSX-2 KS2169 KS2098 Sum ner KS7436-055 KS2185 C eres KS2064 KS7436-055 KS2004 Virginia Plainsman Casino R asm us bu/ac Figure 5. Winter canola seed yields at Bozeman (top) and Kalispell (bottom), MT. The two highest yielding entries were European in both trials. Figure 1. RR winter canola in agronomy trial at Bozeman, MT. This plot was sown at 200 seeds m -2 and was sprinkler irrigated (3.8 inch) to initiate germination on Sep 8. Volunteer spring wheat was controlled with Roundup @ 12 oz/ac. Photo was taken Oct 5, 2007. 0 0.2 0.4 0.6 0.8 1 1.2 1.4 80 197 377 P lantdensity S eedling biom ass 46 das (M g/ha) 0 20 40 60 80 100 120 140 160 180 P lantw eight(m g/plant) Mg/ha mg/plant Figure 6. Total seedling biomass and plant weight had inverse relationships with plant density (plants m -2 ), measured 46 das at Bozeman, MT, Oct 27, 2004. All differences significant @ P < 0.01.

Transcript of Winterhardiness Genetics for the Northern Rockies In 2003, Charlie Rife from KSU sent a collection...

Page 1: Winterhardiness Genetics for the Northern Rockies In 2003, Charlie Rife from KSU sent a collection of Russian winter canola lines that he hoped was the.

Winterhardiness Genetics for the Northern Rockies

In 2003, Charlie Rife from KSU sent a collection of Russian winter canola lines that he hoped was the ‘Holy Grail’ for winterhardiness to test in Montana against his most winterhardy K-State genetics. This rainfed trial was sown directly into standing wheat stubble using low-disturbance disk openers. Fall rain arrived late so seedlings were only at the 1 to 2-lf stage at dormancy. Overwinter survival was very low (Fig. 2).

Bad News: The Russian germplasm collection was not the ‘Holy Grail’.

Good News: Winterhardiness of K-State genetics was likely the best in the world.

Importance of Fall IrrigationIn the 2004 trial it was obvious that we were going to lose yet another rainfed trial due to insufficient fall rain. We decided to rescue the trial with a single application of sprinkler irrigation in mid-Sep 2003 (75 mm = 3 inch). The K-State genotypes showed superior winterhardiness compared with the European checks (data not shown) and the yield of the 7 highest entries averaged > 2500 kg ha-1 (50 bu/ac) (Fig.3).

In 2005 we tested the importance of fall irrigation at Bozeman. Significant rain (1.4 inch) fell in September leading to timely emergence and fall growth, and thus, only minor differences among water regimes (Fig 4.).

Strategies for High Yielding Winter Canola in the Northern Rocky Mountain RegionStrategies for High Yielding Winter Canola in the Northern Rocky Mountain RegionPerry MillerPerry Miller1 1 and Duane Johnsonand Duane Johnson22

Rationale

A successful bio-oil industry will depend on feedstock oil crops with a low cost per unit of production.

Winter canola has high yield potential due to synchrony of plant growth with the environment of the northern Rockies.

Preliminary research has shown that soil water in early September is a key constraint to winter canola survival.

The advent of RR winter canola removes a major source of agronomic risk related to weed management.

ObjectiveTo test the relative importance of 1) fall irrigation, 2) fall seeding date, 3) plant density, and 4) cultivar, for winter canola survival, seed yield, and oil production.

Methods

1) Bozeman (southwest MT) and Kalispell (northwest MT) represent mild winter climates in the Rocky Mountain Front region of Montana. 1971-2000 average annual precipitation was 421 and 516 mm, respectively. Bozeman has a deep silt loam soil and Kalispell has a deep sandy loam soil.

2) Preliminary research has been underway since 2000 at Bozeman and 2002 at Kalispell. It has included cultivar evaluation and agronomic factors such as fall irrigation and seed density.

3) The current 2007 sites were established in Sep 2006 under line-source sprinkler irrigation gradients at both locations. Agronomic factors in the experimental design are:

i. Seeding date (early vs late September)

ii. Cultivar (European RR vs winter hardy non-RR KS line)

iii. Seeding rate (25, 50, 100, 200, and 400 seeds m-2)

We will collect data on emergence rates, plant development rates, crop yield, and oil yield.

Preliminary Research

During 2000-2003 at Bozeman, winter canola cultivar evaluation trials were seeded using low disturbance direct-seeding methods in dense cereal stubble. Our goal was to test the adaptation of winter canola in rainfed no-till systems managed for optimal snow cover. Due to dry fall conditions, the stands emerged late each year and small desiccated seedlings entered the winter in the 0 to 2-lf stage, strongly limiting winter survival. In some years green seedlings were present for 24-48 hr after snowmelt, then quickly rotted.

We concluded that winter canola was not a viable option for rainfed no-till cropping systems because seedling establishment was not timely during drought-prone fall environments.

1Dept. Land Resources and Environmental Sciences, Montana State University, Bozeman, MT; 2Dept. Research Centers, Montana State University, Kalispell, MT

We gratefully acknowledge support from the Montana Ag Experiment Station, the Pacific Northwest Canola Research Program, and the MSU Bio-Based Institute. Collaboration with Charlie Rife at Kansas State Univ. was invaluable. Technical expertise was ably provided by Jeff Holmes, Louise Strang, and Bernard Schaff.

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Figure 2. Winter canola stand densities measured 14 Apr 2003 at Bozeman, MT. Purple bars represent genetic lines from K-State and red lines represent genetic lines from Russia.

European Yield Potential

An unexpected result of the 2005 cultivar evaluation trials in Montana was that the European checks posted greater yields than the K-State genetic lines (Fig. 5). Excellent winter survival occurred at both locations.

Managing Plant Density for Winterhardiness

The 2005 trial at Bozeman showed that the greatest yield came from the lowest plant density (80 plants m-2; Fig. 4). Measurements of seedling biomass in the fall showed that total seedling biomass increased with plant density but that individual plant weight decreased with plant density (Fig. 6). Understanding this relationship may be critical to managing winter canola for optimal winter survival and oil yield in the northern Rockies.

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Figure 3. Seed yield of winter canola at Bozeman, MT, 2004. The 7 entries with dark blue bars were in the highest statistical group. The 3 lowest yielding entries with red bars were from Germany and had limited survival but thin stands were harvested due to weed management with the RR trait. The pink bars were also European entries. LSD (0.05) was 9 bu/ac.

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Figure 4. Seed yields in the Fall Irrigation x Rate of Seeding trial, at Bozeman, MT, 2005. The genetic line KS9835-6-5A-MT was used for this trial.

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Figure 5. Winter canola seed yields at Bozeman (top) and Kalispell (bottom), MT. The two highest yielding entries were European in both trials.

Figure 1. RR winter canola in agronomy trial at Bozeman, MT. This plot was sown at 200 seeds m-2 and was sprinkler irrigated (3.8 inch) to initiate germination on Sep 8. Volunteer spring wheat was controlled with Roundup @ 12 oz/ac. Photo was taken Oct 5, 2007.

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Figure 6. Total seedling biomass and plant weight had inverse relationships with plant density (plants m -2), measured 46 das at Bozeman, MT, Oct 27, 2004. All differences significant @ P < 0.01.