Wild species 17 species of tomatoes and their wild relatives Mutants Mostly single gene mutations...
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Wild species• 17 species of tomatoes and their wild relatives
Mutants • Mostly single gene mutations• Induced and spontaneous
Miscellaneous genetic stocks• Introgression lines • Landraces
A collection of genetic stocks and wild relatives of tomato
C. M. RickC. M. Rick
T G R CT G R CTomato Genetics Resource CenterTomato Genetics Resource Center
Total ≈ 3,600 accessions
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Wild Species• From Columbia to Chile
• Also the Galapagos Islands
• Ranges overlap
S. chilense
Moyle 2008 Evolution
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Extreme Xerophytes
• grafting allows growth in the greenhouse
S. sitiens
The Atacama Desert – one of the driest places on Earth
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Wet Conditions
(photo C. Rick)
S. ochranthum on a stream bank S. juglandifolium seedlings
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Galapagos Island Species
Little or no morphological variation
S. cheesmaniae accessions
Charley Rick
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Variation within SpeciesS. peruvianum fruit from a single population
photo C. Jones
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Valuable Traits in Wild Species
• Bacterial and fungal resistance • Gemini virus resistance
• Drought and salinity tolerance• Cold tolerance and insect resistance
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S. lycopersicoides, grows to 3,600m in Andes of S. Peru and N. Chile
(photo C. Jones)
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Mutants in Cultivated Tomato
• Leaf morphologies and colors• Growing habits• Hormone deficiencies• Floral differences: i.e. male steriles
“Lazy” “Ghost”
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“Lanata, Wooly” “Curl”
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Some mutations are lethal or sterile in the homozygote and must be maintained through the
heterozygote
“double dwarf”
“extreme dwarf”
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Pollinations
“Buzz” pollinating Hand pollinating
• Tailored to the mating system: outcrossers and selfers
• 50 plants/accession grown for outcrossers
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Germplasm Evaluation
Germination Testing
Purity, Identity & Diversity
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Seed Regeneration
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Seed Processing
• Fruit is blended and fermented for 48 hours
• Seeds are flattened on paper to dry
• Packaged and placed in storage
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Seed Storage
• Working collection stored at 6°C
• Germ tested after 10 years
• Long term seed stored at -20°C
USDA TGRC
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Seed DistributionAnnual activity•300 - 500 requests from researchers in over 30 countries•approx. 5,000 seed samples distributed•utilization rate of ~ 100%•seed provided gratis
Common uses•breeding•genetics and genomics • leaf development• fruit ripening• fruit biochemistry•evolutionary studies•education
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Threats to Wild Tomatoes in Native Area
agriculture urbanization
herbivores mining
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Department of Plant Sciences
tgrc.ucdavis.edu