ÒWhat is life? Ó ÒWhat was life? Ó ÒWhat will life be? Ó
Transcript of ÒWhat is life? Ó ÒWhat was life? Ó ÒWhat will life be? Ó
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“What is life?”
Yukawa Symposium
Kyoto, October 19, 2007.
D.W. Deamer, Biomolecular Engineering
University of California, Santa Cruz
“What will life be?”
“What was life?”
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Defining life is like defining any other complex device.
If the device is complex, so must be the definition.
Example: What is a Toyota? Distinguish between city, person, car.
A Toyota is a four wheeled vehicle used for personal transportation.
BUT this also defines a pony cart drawn by a horse:
A Toyota is a four wheeled vehicle with an engine that is used for
personal transportation.
BUT: This could be a pony cart taking an engine to the
mechanic for repair.
A Toyota is a motorized four wheeled vehicle used for personal
transportation in which the engine causes the wheels to rotate
and produce forward motion.
BUT the engine won’t run unless it has fuel.
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A Toyota is a motorized four wheeled vehicle used for personal
transportation in which the engine uses fuel to cause the wheels
to rotate and produce forward motion.
BUT: We need to guide the motion.
A Toyota is a motorized four wheeled vehicle with a steering wheel
that is used for personal transportation in which the engine uses fuel
to cause the wheels to rotate and produce forward motion.
PROBLEM: This definition also fits a Honda.
Also, what is an engine? What is fuel? What is a steering wheel?
MAIN POINT: A Toyota is a complex device and requires a complex
definition.
LIFE is also complex, and the answer to “What Is Life” must be complex.
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What is life?
All life is cellular. The minimal unit of life is a cell.
What is a cell?
A cell is the unit of life, defined as a microscopic compartment
bounded by a lipid bilayer. The compartment contains polymers
called proteins and nucleic acids. The proteins are catalysts, and
the nucleic acids contain genetic information.
BUT: I can make this in the lab, and it is not alive.
What is missing?
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A cell is the unit of life, defined as a microscopic compartment
bounded by a lipid bilayer. The compartment contains polymers called
proteins and nucleic acids. The proteins are catalysts, and the nucleic
acids contain genetic information. The protein catalysts use nutrients
and energy in a process called metabolism to synthesize both proteins
and nucleic acids by polymerization, a process we call growth. When
nucleic acids are synthesized, the genetic information content is
replicated, but not precisely. Errors are called mutations. When a cell
reaches a certain size, it can divide into two daughter cells which may
be identical to the parent cell, but occasionally are different because of
mutations. The differences allow populations of cells to evolve by
natural selection, thereby adapting to changes in their environment.
This group of sentences absolutely defines the simplest unit of life.
Nothing else in the universe fits the definition.
BUT like a Toyota, each of the key words must also be defined: lipid
bilayer, protein, nucleic acid, catalyst, nutrient, energy, polymerization,
genetic, mutation, evolution, natural selection….
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How can we go beyond a simple description,
and understand life at a deeper level?
Origin of life: How did life begin on the early Earth?
Synthesize life: Can we produce artificial life in the laboratory?
We start by asking how life can begin:
Life is not just restricted to the Earth, but is best understood in
terms of astrobiology: Life is part of a universal process.
We must first understand the origin of biogenic elements.
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The biogenic elementsCarbon C
Hydrogen H
Oxygen O
Nitrogen N
Sulfur S
Phosphorus P
Compose >99% of a living cell
WHERE DO THEY COME FROM?
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Carbon synthesis: Triple alpha process in stars at
100 million degrees. Fred Hoyle, 1947.
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Nitrogen and oxygen synthesis in CON cycle
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“Eskimo” nebula “Cat’s Eye” nebula
Planetary nebulas around dying stars
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Interplanetary dust particle (IDP)
Micron size range.
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Ice mantle on grain
UV HMT, amino acids,
ketones, amides,
quinones, amphiphilesAccretion
Comets, IDP, planetesimals
with organic compounds
Dilute solution of organics
In prebiotic global ocean
Late accretionary
infallPlanetary surface
Water, methanol,
CO, ammonia
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How do we know that organic compounds
were delivered to the early Earth?
Because it is still happening today:
Organic compounds are present in
carbonaceous meteorites, IDPs.
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Fusion crust
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Question: Can amphiphilic molecules present in
carbonaceous meteorites form stable
membranes? Answer: YES
Phase image Fluorescence
Encapsulated dye
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THE FIRST CELLS
Cells are molecular systems of boundaries andpolymers - both structures are required for theorigin of cellular life.
Self-assembly of boundary structures
Amphiphilic molecules assemble spontaneouslyinto bilayer structures.
Directed assembly of polymers
Catalyzed growth and replication involving
catalytic polymers and genetic polymers thatcontain sequence information.
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Self Assembly Processes
Single DNA strands -->
Double helix
Nascent proteins --> Folded proteins
Lipid dispersions --> Membranes
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Carbonaceous meteorites provide
samples of prebiotic organics delivered
to the Earth.
What amphiphilic molecules are present?
Monocarboxylic acids 8 - 12 carbons long.
+
+
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WATERHYDROCARBON
CHAINS
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DECANOATE-DECANOL VESICLES
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ENCAPSULATION OF MACROMOLECULES
Fact: Lipid vesicles are impermeable to large molecules.
Question: How could large molecules be captured in a
membrane-bounded compartment boundary to produce
simple protocells?
Dehydration cycles: When an amphiphile is dried in the
presence of a macromolecule, then rehydrated,
vesicles are produced that contain the macromolecule.
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DNA can be easily
encapsulated in
lipid membranes.
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dsDNA (~600 BP) IN DECANOIC ACID VESICLES
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WHERE DID LIFE BEGIN?
Can self-assembly occur in
environmental models of prebiotic
conditions?
Model to be tested: Hydrothermal
regions associated with volcanic
activity.
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Kamchatka geothermal regions in Eastern
Russia are plausible models of the
primitive Earth environment:
High altitude, high latitude, recent
volcanism produce sterile sites for
experimental analysis.
Does organic synthesis occur?
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The RNA World Hypothesis:
The first forms of life used RNA both as genetic
material and as a catalyst (ribozymes)
But how was the first RNA synthesized?
Assume a source of mononucleotides:
How could polymerization reactions be driven?
Use fluctuating wet-dry cycles like the edges
of volcanic hydrothermal ponds.
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RNA + H2O Mononucleotides
(AMP, UMP, GMP, CMP)
RNA hydrolysis in water
Mononucleotides
(AMP, UMP, GMP, CMP)RNA
- H2O
Condensation when water is removed
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AMP
Top view
Side view
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Experimental conditions:
Lipids (prepared as small liposomes) were mixed in
varying mole ratios with mononucleotides (5!-AMP or
UMP) in 0.5 ml water.
The samples were dried and rehydrated up to seven
times. The samples were heated and a stream of carbon
dioxide( ~1 ml/min) was used to remove water vapor.
Variables tested: lipid species, reaction time (30 - 120min), temperature (60 - 90 oC), number of wet-dry cycles.
Polymer synthesis was detected by nanopore analysis,
RiboGreen assays, and end-labeling followed by gelelecrophoresis.
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AMP may polymerize
in fluid lipid
microenvironment
!Go = +5.3 kcal/mol
(Dickson et al. 2000)
Disordered bulk
phase AMP:
Cannot undergo
extensive
polymerization
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RNA-like
polymers are
detected by
end-labeling.
RNA
RNA-32P
Rajamani et al. 2007
Alkaline
phosphatase,
T-4 kinase,
AT32P
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Do the lipid and products
survive multiple cycles at
elevated temperature
(90oC) and low pH?
Examine product
after seven cycles.
Phase microscopy
Fluorescence microscopy,
Ethidium bromide stain for
RNA
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CAN WE PRODUCE ARTIFICIAL LIFE
IN THE LABORATORY?
ENCAPSULATED POLYMERIZATION REACTIONS
RNA synthesis in lipid vesicles
Protein syntheis in lipid vesicles
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aa
RNA synthesisOleic acid vesiclesWalde et al. 1994
RNA synthesisPhospholipid vesiclesChakrabarti et al. 1994
ADP
RNA
PNPase
ADP
ADP
RNA
PNPase
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TRANSCRIPTION: DNA --> RNA
Encapsulated T7 RNA polymerase.
Uses DNA template, NTP substrates to
transcribe base sequence from DNA to RNA.
Monnard et al. Phil. Trans. B 2007
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T7 RNA polymerase
ATP
UTP
CTP
GTP
DMPC vesicle
RNA
Transient defect
Phage DNAtemplate
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RNA synthesized in vesicles
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ENCAPSULATED TRANSLATION
Approach:
Capture cytosolic components of disrupted E. coli.
Translation system composed of ribosomes,
tRNAs, amino-acyl tRNA synthetases etc.
Include desired mRNA, 20 amino acids, ATP
Yu et al. 2001; Nomura et al. 2003
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Vesicle with green
Fluorescent protein (GFP)Initial state
Time
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a
Amino acids
ATP
Amino acids
ATP
GFPGFP
GFPGFP
a-HL
Ribosome
mRNA
Noireaux and Libchaber, PNAS 2004
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ENCAPSULATED GENETIC CASCADE
Ishikawa et al. 2004, FEBS Letters 576, 387-390.
Plasmid DNA was prepared containing genes for GFP
and T7 polymerase, both under control of promoters.
The plasmid was incorporated in large liposomes
containing translation system components, amino acids
and ATP.
GFP fluorescence was monitored by flow cytometry.
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Reaction mixtureBuffer (200 mM Tris acetate), 17 mM Mg(OAc)2, 2.5% PEG8000,
ATP, 7.9 mM, GTP, UTP, and CTP, 0.88 mM
20 amino acids, 0.325 mM each
tRNAs, 0.17 mg/ml
16 nM of template DNA (pTH plasmid, 9000 bp)8.0 µl of E. coli S-30 cell extract.
Encapsulation10 µl of the reaction mixture added to the lyophilized liposomes
Egg phosphatidylcholine-cholesterol, 1:1Mixed by pipetting to form large liposomes, 1 - 10 µm diameter
Final lipid concentration 120 mM
RNAse added to inhibit translation external to liposomes
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GFP SYNTHESIS, FLOW CYTOMETRY
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RESULTS OF THE GENETIC CASCADE
1. T7 RNA polymerase is synthesized from its gene.
2. The polymerase binds to its promoter to transcribe
GFP mRNA
3. GFP is translated by the encapsulated ribosomes.
4. Approx. 500 copies of GFP were synthesized per gene.
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Is this system “alive”?
NO. Only two proteins are synthesized.
Everything else -- ribosomes, enzymes,
lipid boundaries -- is left behind.
THE CHALLENGE NOW:
Use self-assembly to reconstitute
cellular assemblies that grow and
reproduce.
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aa
Synthetic cell, initial state
DNA, ~100 genes
20 tRNAs20 aminoacyl-tRNA synthetasesRNA polymeraseHemolysin pores
Ribosomes
Nutrients
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~100 genes required in artificial cell genome
Translation system
20 tRNAs
3 rRNAs (5S, 16S, 23S)
55 ribosomal proteins
20 aminoacyl tRNA synthetases
Nucleic acid synthesis
RNA polymerase
DNA polymerase
Membrane growth - phospholipid synthesis
Acyl transferaseTransport - "-Hemolysin channel
NOT VERY PLAUSIBLE IN PREBIOTIC ENVIRONMENTS !
THE FIRST FORMS OF LIFE MUST HAVE BEEN SIMPLER.
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Initial synthesis of catalytic and replicating polymers?
Assembly of translation system (ribosomes)?
Replication of the transcription and translation systems.
Sources of energy and nutrients?
Membrane growth to accommodate internal growth?
Regulatory feedback between internal polymer growth
and growth of the membrane.
Division into smaller systems.
We can be optimistic: The first cellular forms of life
spontaneously overcame all of these hurdles!
REMAINING QUESTIONS:
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UC Santa Cruz NASA Ames
Pierre-Alain Monnard Lynn RothschildCharles ApelSudha RajamaniSara SingaramTrishool Namani
Supported by NASA Exobiology program,1985 - present.