Wen-Hung Twan.ppt [相容模式] -...
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Hormones and reproduction in Hormones and reproduction in scleractinian coralsscleractinian corals
WenWen--Hung TwanHung Twan1,21,2, , and PeiPei--Jie MengJie Meng1,31,3
1 National Museum of Marine Biology & Aquarium, Pingtung 944, Taiwan2 Institute of Marine Biotechnology, National Dong Hwa University,
Pingtung 944, Taiwan 3 Institute of Marine Biodiversity and Evolution, National Dong Hwa
University, Pingtung 944, Taiwan
Introduction
Patterns of Sexual reproductionBrooderBroadcaster (broadcast spawner)
Synchronous mass spawning
Environmental cuesSeawater temperaturespLunar phase
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Coral and Seawater CollectionCoral and Seawater Collection
Euphyllia glabrescens
Euphyllia ancoras
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Ready to spawning
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mesentery
gonad
mesentery
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Sex Steroids inSex Steroids in E. ancoraE. ancora
Testosterone and Estradiol-17ββ
Sex steroids have significant effects
OH OH
on gonadal development and gametogenesis
O
TestosteroneTestosterone
HO
EstradiolEstradiol--1717ββ
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Polyps were collected by scrapingPolyps were collected by scraping
Tissues were homogenizedTissues were homogenized
Characterization of Steroids in CoralCharacterization of Steroids in Coral
Tissues were homogenizedTissues were homogenized
SupernatantSupernatant
Alumina Gel ChromatographyAlumina Gel Chromatography
Extracted with diethyl etherExtracted with diethyl ether
rp-HPLC
Radioimmunoassay
g p yg p y
Alumina Gel Chromatography
Solvent Systemumn
Solvent System
TestosteroneTestosteroneor
Alu
min
a G
el C
olu
Coral extract
1st 4th3th2nd 5th 6th
EstradiolEstradiol--1717ββ
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The retention time of the testosterone and coral extract in the HPLC
30
10
20
30
oste
rone
(pg/
mg
tissu
e)
20
40
60
80
12
mv
11
7
[1] cortisone[2] cortisol[3] 11-ketotestosterone[4] 11-deoxycortisol[5] 20 βS
0 10 20 30 40 50 60 700
Test
o
0 10 20 30 40 50 60 70
0
201098654321
min
[6] androstenidione[7] deoxycorticosterone[8] testosterone[9] 17α, 20β -dihydroxypregnenone[10] estradiol[11] 17α-hydroxyandrostenone[12] progesterone.
Sex steroids were existed in E. ancora and E. glabrescens.
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The annual Profiles of Sex Steroids The annual Profiles of Sex Steroids in in E. ancoraE. ancora
20
30
40
50 a
b
ababab
ab
tero
ne (n
g/g
tissu
e) a
A
The annual changes of Sex steroid hormonesconcentrations in E. ancora.
Mar-98 Apr-98 May-98 Jul-98 Oct-98 Jan-99 Apr-99 May-990
10 b
Test
ost
500
600
700 a
ng/g
tiss
ue)
aB
spawning spawning
Mar Apr May Jul Oct Jan Apr May0
100
200
300
400
Estra
diol
-17β
(n
b
c bccbcbc
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30
40
50
60
70ne
(ng/g
tis
sue)
The changes of Sex steroid hormones concentrations in E.glabrescens during the spawning period
300
350
400
450
tiss
ue)
0
10
20
Apr May Jun Jul Aug Sep
Test
ost
eron
0
50
100
150
200
250
Apr May Jun Jul Aug Sep
Est
radio
l (ng/g
Conjugated SteroidsConjugated Steroidsj gj g
Conjugation (formation of hydrophilic molecules) is an important step in steroid catabolism
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Steroids and conjugated steroids measured in seawater collected during the spawning periods
During Mass SpawningSpawning
Seawater (pg/ml)
Estradiol
Sea SurfaceAround Coral
20 3 ± 3 4
5.8 ± 0.9 2.7 ± 0.7
5 9 ± 0 8
One dayafter
Mass Spawning
━
One dayprior to
Mass Spawning
━
Testosterone glucuronide
Testosterone
Estradiol glucuronide
Average value (mean ± SEM) in seawater (3 yr)
20.3 ± 3.4 5.9 ± 0.8
━ ━
━0.8 ± 0.2 ━
━
━
━
━
━
Steroids and conjugated steroids measured in coral and seawater collected during the E. ancora spawning periods
During Mass Spawning
Seawater (pg/ml)
Estradiol glucuronide
Estradiol
Sea SurfaceAround Coral
642.8 ± 25.4
20 3 ± 3 4
Coral(ng/g tissue)
228 8 ± 9 8
5.8 ± 0.9 2.7 ± 0.7
5 9 ± 0 8
Testosterone glucuronide
Testosterone
Estradiol glucuronide
Average value (mean ± SEM) in seawater (3 yr) and coral (3 colonies, 2yr)
20.3 ± 3.4228.8 ± 9.8
10.5 ± 2.0
42.7 ± 3.0
5.9 ± 0.8
━ ━
━0.8 ± 0.2
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Steroids and conjugated steroids measured in seawater (around coral) collected during the spawning periods
During Spawningg p g
Seawater (pg/ml)
Estradiol
E. glabrescensE. ancora
20 3 ± 3 4
5.8 ± 0.9 2.3 ± 0.3
Release larvaeRelease eggs
Testosterone glucuronide
Testosterone
Estradiol glucuronide
Average value (mean ± SEM) in seawater (3 yr)
20.3 ± 3.4 ━
━ ━
━0.8 ± 0.2
Thank you for your attentionThank you for your attention