Welcome We are pleased to welcome you to Venom Week VI, to be held on March 14-17, 2018 at Texas A&M University-Kingsville, Peacock Auditorium and Student Union Building at 700 University Blvd, Kingsville, TX 78363. As symposium Chair, Director of the National Natural Toxins Research Center and Associate professor of Chemistry at Texas A&M University-Kingsville along with Steven A. Seifert, MD, FAACT, FACMT organizer and past chair of Venom Week, and Daniel E. Keyler, PharmD, FAACT President of the North American Society of Toxinology who are on the planning committee, we are pleased that you have decided to join us and bringing your expertise to this gathering. Venom Week was founded in 2001. Venom Week VI is the International Scientific Symposium of all things venomous; emphasizing on venomous animals, the venom they produce, and the management of envenomations. This North American scientific meeting is intended for clinicians, basic scientists, field researchers, herpetologists, zookeepers and others with an interest in venomous animals. The world of toxinology is an exciting area in which to work/study/play, and we’ll continue to meet and bring inspired people together in symposiums like this, to guarantee our field remains at the cutting edge. Below is a loose itinerary of some of the topics you can expect and what we hope to achieve over the next few days. Each and every one of you, including our young scientists, is truly our greatest asset today and tomorrow, and we could not accomplish what we do without your encouragement and guidance. Throughout this conference, I ask you to stay involved, keep us proactive and help us shape the future of toxinology. My personal regard and thanks goes out to all of you.

Objectives

At the end of the conference, the participants will be able to:

• Characterize the medically important reptiles and arthropods involved in envenomation

• Describe the medical consequences of envenomation

• Study and review the principles and practice of antivenom use

Who Should Attend It is anticipated that approximately 250 professionals in the fields of emergency medicine, medical toxicology, veterinary medicine, nursing, pharmacy, arachnology, antivenom manufacturing, and captive collections management will attend the 4 day-long conference.

All proceedings will be informal.

Please plan to dress casually and

bring a sweater or jacket for your

comfort.

Lodging Information Holiday Inn Express & Suites 2400 S Hwy 77, Kingsville, TX 78363 (361) 592-8333 4.4 Miles to Peacock Auditorium Reserved Rooms: Double Rooms (25) *February 13, 2018*

Hampton Inn 2489 S Hwy 77, Kingsville, TX 78363 (361) 592-9800 4.7 Miles to Peacock Auditorium Reserved Rooms: King Rooms (21) & Queen Double Rooms (22) Americas Best Value Inn (Best Western) 2402 E King Ave, Kingsville, TX 78363 (361) 595-5656 3.4 Miles to Peacock Auditorium Reserved Rooms: 25 rooms available *February 28th, 2018* Newman Hall 1325 W Corral Ave, Kingsville, TX 78363 (361) 221-1342 (361) 288-3661

0.6 Miles to Peacock Auditorium *Note: Bedding is not provided* Reserved Rooms: 2 double units (2x2) $15 per person per night

2 single units (2x1) $21 per person per night

*Rooms available until capacity is reached* Comfort Inn (Quality Inn) 505 N Hwy 77, Kingsville, TX 78363 (361) 516-1120 3.7 Miles to Peacock Auditorium Super 8 105 S Hwy 77, Kingsville, TX 78363 (361) 592-6471

3.3 Miles to Peacock Auditorium Motel 6 100 North US 77, Kingsville, TX 78363 (361) 489-3516

3.8 Miles to Peacock Auditorium

Econo Lodge 221 S. US Highway 77 Bypass, Kingsville, TX 78363 (361) 592-5251

3.5 Miles to Peacock Auditorium

Contact Hours: Upon request through each participant’s registration form.

VENOM WEEK VI AGENDA Tuesday, March 13, 2018

12:00 - 5:00 pm PRE-REGISTRATION A. L. Kleberg Hall Room 101 975 W. Ave. B., Kingsville, TX

6:00 - 8:00 pm RECEPTION Conner Museum, Texas A&M University-Kingsville 905 W. Santa Gertrudis Ave., Kingsville, TX

VENOM WEEK VI AGENDA Wednesday, March 14, 2018

7:30 REGISTRATION/CONTINENTAL BREAKFAST Student Union Building 2nd Floor, Ballroom B

8:00 WELCOME/OVERVIEW Elda E. Sánchez, PhD, Chair of Venom Week VI, Texas A&M University-Kingsville/NNTRC

8:10 Keynote: How Can Venom Help Us To Understand And Treat Pain? Glenn King, PhD, The University of Queensland, Brisbane, Australia

8:50 Keynote: Crotalus durissus terrificus (Viperidae; Crotalinae) Envenomation And Treatment With Antivipmyn TRI® Antivenom Daniel Keyler, PharmD, University of Minnesota, Minneapolis, MN

9:10 Breaking The Dichotomy: Type 3 Venom Expression In C. v. viridis/C. o. concolor Natural Hybrids Stephen Mackessy, PhD, University of Northern Colorado, Greeley, CO

9:30 Conservation Efforts For The Armenian Viper, Montivipera Raddei: Spatial Ecology, Protected Areas And More Jeff Ettling, PhD, Sedgwick County Zoo, Wichita, KS

10:00 BREAK 10:20 Lachesis Envenomation

Lorenzo R. Pelly, MD, Valley Baptist Medical Center, Brownsville, Texas, USA 10:40 Asp Viper Envenomation In A 4-Year Boy

Cyrus Rangan, MD, Children’s Hospital, Los Angeles, CA 11:00 Keynote: Proteomic Studies On Micrurus (Coral Snakes) Venoms Reveal A Dichotomy Of

Phenotype Bruno Lomonte, PhD, Instituto Clodomiro Picado, Universidad de Costa Rica, San Jose, Costa Rica

11:30 The Evolutionary And Biophysical Origin Of Mojave Toxin Carl Whittington, PhD, Florida State University, Tallahassee, FL

12:00 LUNCH Student Union Building 2nd Floor, Ballroom B

1:00 The Validity, Reliability And Minimal Clinically Important Difference Of The Patient Specific Functional Scale In Snake Envenomation Charles Gerardo, PhD, Duke University, Durham, NC

1:20 Low Socioeconomic Development Impacts Treatment Of Snake Envenomation In Brazil Jinny Ye, PhD, Duke University, Durham, NC

1:40 Occupational Snakebites: A Retrospective Cohort Of Patients Reported To The Toxic North American Snakebite Registry Steven Seifert, MD, University of New Mexico, Albuquerque, NM

2:20 Long Term Effects Of Snakebite, Preliminary Results Of A Subjective Survey Joe Pittman, RN, Florida Snakebite Institute, Tampa, FL

2:40 When Swiss Cheese Almost Kills A Child: A Particular Case Of Severe Envenomation Of

A Child By Eastern Coral Snake Benjamin Abo, MD, University of Florida College of Medicine, Gainesville, FL

3:00 BREAK 3:20 Keynote: Third Generation Antivenomics: Pushing The Limits Of The In Vitro Preclinical

Assessment Of Antivenoms Juan Calvete, PhD, Instituto de Biomedicina de Valencia, Spain

4:00 Keynote: It’s A Snake-Bite-Snake World Sean Bush, MD, East Carolina University, Greenville, NC

4:50 ANNOUCEMENTS 5:10 ADJOURN

VENOM WEEK VI AGENDA Thursday, March 15, 2018

7:30 REGISTRATION/CONTINENTAL BREAKFAST Student Union Building 2nd Floor, Ballroom B

8:00 WELCOME/OVERVIEW Elda E. Sánchez, PhD

8:10 Keynote: Exploring The Basis Of The (Uncommon) Direct Hemolysis Caused By Phospholipases A2 Of Micrurus Fulvius Venom Bruno Lomonte, PhD, Universidad de Costa Rica, San Jose, Costa Rica

8:40 Keynote: Divergent Roads: Distinct Evolutionary Trends Across Crotalus Recognized Through Multi-Omic Approaches Juan Calvete, PhD, Instituto de Biomedicina de Valencia, Spain

9:10 Tumescent Contravenom: Dilute Subcutaneous Epinephrine Delays Systemic Absorption of Neutotoxic Cobra Venom Jeffrey Klein, MD, University of California, Irvine, CA

9:30 From Syndrome to Venome: Inferring Venom Composition from Clinical Symptoms of Rattlesnake Envenomation William K. Hayes, PhD, Loma Linda University, Loma Linda, CA

10:00 BREAK 10:20 Antivenom Administration was Associated with Shorter Opioid Use in Copperhead

Snakebite Patients Victoria Anderson, MD, Rocky Mountain Poison and Drug Center, Denver, CO

10:40

Opossum Peptide Neutralizes Sub Saharan Africa Viper Venoms Claire Komives, PhD, San Jose State University, San Jose, CA

11:00 Keynote: Opossums Vs. Vipers: Molecular And Functional Characterization Of A Coevolutionary Arms Race Danielle H. Drabeck, PhD Candidate, University of Minnesota, Minneapolis, MN

11:30 Biochemical Warfare: The Coevolution Of Venom And Venom Resistance Among Small Mammals Kelly Robinson, MS, San Diego State University, San Diego, CA

12:00 Lunch (Poster Viewing and Judging) Student Union Building 2nd Floor, Ballroom A & B

1:00 Eco-Epidemiological Circumstances, Pre-Admission Histories And Assessment Of Risk Factors Of Envenoming And Death Due To Krait And Russell’s Viper Bites In Southern Nepal Deb P. Pandey, PhD, Kaligandi Health Foundation, Kawasoti, Nawalparasi, Nepal

1:20 Keynote: Beak Of The Snake: Causes Of Venom Variation In Sistrurus Rattlesnakes

Lilse Gibbs, PhD, Ohio State University, Columbus Ohio

2:00 Venom toxins to drugs: anti-thrombotic and anti-metastasis compounds from snake venoms Stephen Mackessy, PhD, University of Northern Colorado, Greeley, CO

2:30 Keynote: Synergism Among Genomic, Transcriptomic, And Proteomic Approaches To The Study Of Venom Composition And Evolution Darin Rokyta, PhD, Florida State University, Tallahassee, FL

3:10 BREAK 3:20 Keynote: In Vivo Neutralization Of Black Mamba Venom Using A Human IgG Antibody

Cocktail Andreas Laustsen, PhD, Technical University of Denmark, Copenhagen, Denmark

4:00 Self-Immunization Gus Gross, MD, Valley Baptist Medical Center, Brownsville, TX

4:20 The VIPRBITEM cohort experience with venom vaccination and hyper-immunity: risks, safety, and efficacy Brian Hanley, PhD, Butterfly Science, Davis, CA

5:10 ADJOURN 6:30 Tex-Mex Dinner and Mariachi Night at the Tio Kleberg and Janelle Wildlife Research Park

VENOM WEEK VI AGENDA Friday, March 16, 2018

7:30 REGISTRATION/CONTINENTAL BREAKFAST Student Union Building 2nd Floor, Ballroom B

8:00 WELCOME/OVERVIEW Elda E. Sánchez, PhD

8:10 Keynote: Venom Toxins To Drugs: Anti-Thrombotic And Anti-Metastasis Compounds From Snake Venoms Stephen P. Mackessy, PhD, University of Northern Colorado, Greeley, CO

8:50 Synthesis Of Ω-Theraphotoxin-Pv3a, An Aedes Aegypti And Anopheles Albimanus Larvicidal Disulfide Bridged Peptide From The Colombian Tarantula Pamphobeteus Verdolaga (Araneae:Theraphosidae) Sebastian Estrada-Gomez, PhD, Universidad de Antioquia, Medellín, Colombia

9:20 A Venom Peptide For Treating Dravet Syndrome Epilepsy Glenn King, PhD, The University of Queensland, Brisbane, Australia

10:00 BREAK 10:20 Crotalus Oreganus Concolor (Viperidae; Crotalinae): A Case Of Envenomation With

Venom Analysis From The Envenoming Snake And A Diagnostic Conundrum Of Myo- Neurological Symptoms Daniel Keyler, PharmD, University of Minnesota, Minneapolis, MN

11:00 Awards, Presentation Of New NAST Officers, And Words From New NAST President Dan Keyler, Carl-Wilhelm Vogel, Elda E. Sánchez, Steven Seifert

11:30 Development Of A Therapeutic Agent From A Component Of Cobra Venom For Treatment Of Diseases With Complement Pathogenesis Carl-Wilhelm Vogel, MD, PhD, University of Hawaii Cancer Center, Honolulu, HI

12:00 Lunch Student Union Building 2nd Floor, Ballroom B

1:00 Keynote: On a Mission: Venom Production, Education and Conservation Challenges Kristen Wiley, Kentucky Reptile Zoo, Slade, KY

1:40 Considerations and Challenges in Designing Snakebite Protocol

Katherine Shoemaker, Kentucky Reptile Zoo, Slade, KY 2:00 Snakebites And Antivenom Management In Nepal

Deb P. Pandey, PhD, University of Peradeniya, Sri Lanka 2:20 To Cut Or Not To Cut

Alejandro A. Betancourt, MD, Valley Baptist Medical Center, Harlingen, TX 2:40 The Acute Effects Of Snake Venom CRiSP Toxins On Blood And Lymphatic Endothelial

Cell Permeability: New Insights Into The Pathophysiology Of Snakebite Montamas Suntravat, PhD, Texas A&M University-Kingsville/NNTRC, Kingsville, TX

3:00 BREAK 3:20 Current Progress Towards The Next Generation Of Antivenoms

Andreas Laustsen, PhD, Technical University of Denmark, Copenhagen, Denmark 3:50 The Neutralization Efficacy Of Expired Polyvalent Antivenoms: An Alternative Option

Chesney Migl, BS, Texas A&M University-Kingsville/NNTRC, Kingsville, TX 4:10 Neurotoxic Snakebite In Guinea: Addressing A Complex Public Health Crisis

Jordon Benjamin, PhD, Whitman University, Seattle, WA 4:30 Attitude, Knowledge And Awareness Of People Towards Snake, Snakebite And Snake

Conservation In Rupandehi District, Nepal Kamal Devkota, Nepal Toxinology Association

5:10 ADJOURN 6:00 NAST Board Meeting

A.L. Kleberg Hall, Room 101

VENOM WEEK VI AGENDA Saturday, March 17, 2018

7:30 REGISTRATION/CONTINENTAL BREAKFAST Student Union Building 2nd Floor, Ballroom B

8:00 WELCOME/OVERVIEW Elda E. Sánchez, PhD

8:10 Early Antivenom Treatment Improved Time to Recovery in Patients with Copperhead Envenomation Victoria Anderson, MD, Rocky Mountain Poison and Drug Center, Denver, CO

8:50 Keynote: Current Veterinary Management of Pit Viper Envenomation in the United States Michael E. Peterson, DVM, MS, Reid Veterinary Hospital, Albany, OR

9:20 The Perplexing Distribution of Type A and Type B Venom Phenotypes in Rattlesnakes William Hayes, PhD, Loma Linda University, Loma Linda, CA

9:40 Polyclonal Antibodies Against A Recombinant Disintegrin: Their Diverse Uses Elda E. Sánchez, PhD, Texas A&M University-Kingsville/NNTRC, Kingsville, TX

10:00 BREAK 10:20 Inhibitory Effects Of Triterpenic Acids On Enzymatic And Pharmacological Activities Of A

Snake Venom Metalloproteinase: Insights From Docking And Molecular Dynamics Lina Preciado, PhD, Universidad de Antioquia, Medellín, Colombia

10:50 The Genetics of Prey-Driven Character Optimization In Venomous Snakes Michael Hogan, PhD Student, Florida State University, Tallahassee, FL

11:10 Keynote: Persistent Pit Viper Envenomation In A Cat Michael Schaer, DVM, University of Florida, Gainesville, FL

11:50 Looking Forward….the Next Venom Week Elda E. Sánchez & Daniel Keyler

12:20 ADJOURN

POSTER PRESENTATIONS Thursday, March 15, 12:00pm

Student Union Building (SUB) P-1 Don’t pet your Gaboon viper!

Joe Pittman, RN, Florida Snakebite Institute, Tampa, FL P-2 Harnessing Snake Venoms To Make T. brucei For Ever Go To Sleep

Andreas Laustsen, PhD, Technical University of Denmark, Copenhagen, Denmark P-3 Sting Ray Sting

Fernando Moran, MD, Valley Baptist Medical Center, Brownsville, TX P-4 Snakebites and Antivenom Management in Nepal

Chhabilal Thapa Magar, MD, Kaligandaki Foundation Pvt. Ltd. PhD-1 Venom And Drosophila: Response Variation In Experimental Prey

Micaiah Ward, PhD Student, Florida State University, Tallahassee, FL PhD-2 The Design of Complex Weapons Systems in Scorpions: Sexual, Ontogenetic, and

Interspecific Variation Gerad Fox, PhD Student, Loma Linda University, Loma Linda, CA

PhD-3 Characterization Of The Venom From The Eastern Soil Centipede (Scolopocryptops sexspinosus) Utilizing Venom-Gland Transcriptomics And Venom Proteomics Schyler Ellsworth, PhD Student, Florida State University, Tallahassee, FL

PhD-4 Characterizing Toxin Diversity In The Venom-Gland Transcriptome And Venom-Proteome Of The Florida Blue Centipede, Hemiscolopendra marginata Gunnar Nystrom, PhD Student, Florida State University, Tallahassee, FL

DVM-1 Dose Dependent Effects Of CORM-2 On Mitigating Rattlesnake Venom Induced Coagulopathy In Canine Whole Blood Jennifer Schefski, DVM Candidate, Colorado State University, Fort Collins, CO

MS-1 Isolation And Characterization Of C-Type Lectins From Southern Pacific Rattlesnake On Human Dermal Lymphatic Endothelial Cells Shelby Szteiter,BS, MS Student, Texas A&M University-Kingsville/NNTRC, Kingsville, TX

MS-2 Purification And Characterization Of Cysteine Rich-Secretory Proteins (CriSPs) From The Venom Of The Southern Pacific Rattlesnake (Crotalus oreganus helleri): Their Role On Blood And Lymphatic Endothelial Cell Permeability Jessenia Marquez, BS, MS Student, Texas A&M University-Kingsville/NNTRC, Kingsville, TX

MS-3 Neutralization Of Snake Venom Disintegrins And Metalloproteases By Polyclonal Antibodies Against Disintegrins Ilse Diego, BS, MS Student, Texas A&M University-Kingsville/NNTRC, Kingsville, TX

UG-1 Expression, Purification, And Characterization Of The New Recombinant Crotamine Isoform From The Venom Gland Of Crotalus oreganus helleri On Antimicrobial Activity Roland M. Montemayor, UG Student, Texas A&M University-Kingsville/NNTRC, Kingsville, TX

UG-2 Antibacterial Activity Of 24 Different Snake Venoms From Families Viperidae And Elapidae Oscar Sanchez, UG Student, Texas A&M University-Kingsville/NNTRC, Kingsville, TX

UG-3 Purification And Characterization Of Snake Venom Cysteine-Rich Secretory Proteins (svCRiSPs) From 5 Different Species Of North America Venomous Snakes Andrew Morales, UG Student, Texas A&M University-Kingsville/NNTRC, Kingsville, TX

UG-4 Interspecific Venom Variation of Centruoides vittatus Laura Koffinas, UG Student, Florida State University, Tallahassee, FL

UG-5 Venom Degradation Study Rachel Saul, UG Student, Florida State University, Tallahassee, FL

Invited Speakers Cyrus Rangan, MD

California Poison Control System; Children's Hospital Los Angeles

La Crescenta, CA

Danielle Drabeck, Academic Evolutionary Biologist

Saint Paul, Minnesota

Drain Rokyta, PhD

Florida State University

Tallahassee, FL

Juan Calvette, PhD

Instituto de Biomedicina de Valencia Spain

Kristen Wiley, MS

Kentucky Reptile Zoo

Slade, Kentucky

Stephen Mackessy, PhD

University of Northern Colorado

Greeley, CO

Micheal Schaer

University of Florida, College of Veterinary Medicine

Gainesville, FL

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