Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351

Supplemental Data Set 1. Multiple sequence alignment of VIP proteins used to generate phylogeny in Fig. 2B.AnVIP1 1 ----------------------------------------------------------------------AtVIP1 1 ----------------------------------------------------------------------CeVIP1 1 ----------------------------------------------------------------------CrVIP1 1 ----------------------------------------------------------------------CsVIP1 1 ----------------------------------------------------------------------DmVIP1 1 ----------------------------------------------------------------------HsVIP1 1 ----------------------------------------------------------------------MpVIP1 1 ----------------------------------------------------------------------OlVIP1 1 ----------------------------------------------------------------------OsVIP1 1 ----------------------------------------------------------------------OtVIP1 1 ----------------------------------------------------------------------PtVIP1 1 ----------------------------------------------------------------------ScVIP1 1 -----------------------------------------------MSGIKKEPIESDEVPQQETKNNLSPVIP1 1 ----------------------------------------------------------------------TpVIP1 1 ----------------------------------------------------------------------UmVIP1 1 ----------------------------------------------------------------------VCVIP1 1 ----------------------------------------------------------------------AtVIP2 1 ----------------------------------------------------------------------CrVIP2 1 MPRARPTEAAAAAAEAAAAVGAAGAAAAGARQPMQSNGSGSSSGGGGGGGGGRVGAECADGRGPTGDFRAHsVIP2 1 ----------------------------------------------------------------------OsVIP2 1 ----------------------------------------------------------------------VcVIP2 1 QQQQSRRLISARRSDPCAGPQGGPSATATATAGSSAHAASETCAGLAAAARRPAGGGTAGGSKAAPAPQD

AnVIP1 1 ----------------------------------------------------MDRTHNTTSHGPDSSETPAtVIP1 1 ----------------------------------------------------------------------CeVIP1 1 ----------------------------------------------------------------------CrVIP1 1 ----------------------------------------------------------------------CsVIP1 1 ----------------------------------------------------------------------DmVIP1 1 ----------------------------------------------------------------------HsVIP1 1 ----------------------------------------------------------------------MpVIP1 1 ----------------------------------------------------------------------OlVIP1 1 ----------------------------------------------------------------------OsVIP1 1 ----------------------------------------------------------------------OtVIP1 1 ----------------------------------------------------------------------PtVIP1 1 -------------------------MEETEQGHPTKPADVAGLRGSQVSDSEGIAESPENLALAPVALSAScVIP1 24 PSAPSEMSPLFLNKNTQKAMQSIAPILEGFSPKTSASENMSLKLPPPGIQDDHSEENLTVHDTLQRTISTSPVIP1 1 ----------------------------------------------------------------------TpVIP1 1 ----------------------------------------------------------------------UmVIP1 1 ----------------------------------------------------------------------VCVIP1 1 ----------------------------------------------------------------------AtVIP2 1 ----------------------------------------------------------------------CrVIP2 71 AAGSAAAGDAAGGEATASPTPGATASGSAAAGGTAAAAGSGGGGGGRGSFMRCSSAPVWQLQPSLQPPAAHsVIP2 1 ----------------------------------------------------------------------OsVIP2 1 ----------------------------------------------------------------------VcVIP2 71 LSTAILLGDDSPACAPAAAAVGGAAGLGAGEGLPVTEPDAAGRGADGGLRRPPGLAAGVRVTPQQQVSVA

AnVIP1 19 LKPTASATNLGLEEEKTSARFSCRSSASSSSKGYPHTVQVSQSKASQSDNVTDVPQPGRGARSSTRSSSRAtVIP1 1 ----------------------------------------------------------------------CeVIP1 1 ----------------------------------------------------------------------CrVIP1 1 ----------------------------------------------------------------------CsVIP1 1 --------------------------------------------------------------------MADmVIP1 1 ----------------------------MSYTELESGYQDISQQHQQQNPHQSQPQQRVGFYLGLQDGNGHsVIP1 1 ----------------------------------------MWSLTASEGESTTAHFFLGAGDEGLGTRGIMpVIP1 1 ----------------------------------------------------------------------OlVIP1 1 ------------------------------------------------------------------MSEHOsVIP1 1 ----------------------------------------------------------------------OtVIP1 1 ----------------------------------------------------------------------PtVIP1 46 VPEEAVASLETTSLPKAKEGSGSKATSRPCLVSRHQSRDSSHDLDLDAMDAISETKKSRHSKANTPGANAScVIP1 94 ALGNGNNTNTVTTSGLKKADSESKSEADPEGLSNSNIVNDADNINSISKTGSPHLPQGTMDAEQTNMGTNSPVIP1 1 --------------------------------------------------------------MIQNASHLTpVIP1 1 ----------------------------------------------------------------------UmVIP1 1 ----------------------------------------------------------------------VCVIP1 1 ----------------------------------------------------------------------AtVIP2 1 ----------------------------------------------------------------------CrVIP2 141 AASGPAAPATPGNVAG-ASATSTGGATPIRDISGRPSSSGCSPSSTSGSSSGGAGSSCTRTSTCDSGGGSHsVIP2 1 -------------------------------------------------MSEAPRFFVGPEDTEINPG--OsVIP2 1 ----------------------------------------------------------------------

1

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351VcVIP2 141 ASPGCGVGGTAGCGGQQLPQPHQAWRRSFPRSKSAPSWPPLALNLNLSGGGNGGGSGCNGDGDDEEGPSC

2

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 89 APRRLSGSTAASSMSEVEPPPAFLGKIGVCALDVKARSK------PSQNILTRLQSKG--DFEVIVFGDKAtVIP1 1 -----------MEMEEGASGVGEKIKIGVCVMEKKVKCGSEVFSAPMGEILDRLQSFG--EFEILHFGDKCeVIP1 1 --------MAHKGTESKEQIWPYKITIGICAMNRKATSK------PMRAIMKKIIDFYGQWVDSFIFPEQCrVIP1 1 ---MRRLFKGLFGKVDGQNQNLPKIRVGVCAMDKKARSK------PMKEILERLTAWG--EFEVVIFGDDCsVIP1 3 SVMSKGISIESEPWLADHDSSLPKIHIGICAMDKKAHSR------PMSAIVERLEAYG--EFDIKYFGDEDmVIP1 43 DTDFGDSNDGMDSDTSTSSSNSKQVVVGICAMAKKTQSK------PMKEILTRLGEFE--FIKLVTFEENHsVIP1 31 GMRPEESDSELLEDEEDEVPPEPQIIVGICAMTKKSKSK------PMTQILERLCRFD--YLTVVILGEDMpVIP1 1 -----------------MPLRYRKVTVGVCAMDKKAKSK------AMLEILNRLEDFP--EFEIITFGDDOlVIP1 5 AAPTRDARKQSSRSGASSASKHPIVTLGVCAMDKKSRSS------AMREILTRIESFG--EFEIVIFGDEOsVIP1 1 -----------MERRNDGCDGGGKIKIGVCVMEKKVWCS------PMEQILERLRAFG--EFEIIIFGDKOtVIP1 1 -------------------MKHPAVILGVCAMDKKSRSS------AMREILTRIEAFG--EFEIVVFGDEPtVIP1 116 TKPKALPNIPAADNHDDDESYKDRIRLGICAMDKKARSK------PMAEILSRLDES---LFQVVFFGDDScVIP1 164 SVPTSSASSRKSSTSHPKPRLPKVGKIGVCAMDAKVLSK------PMRHILNRLIEHG--EFETVIFGDKSPVIP1 9 TSIDTESSTRTASPVSSIVTPTKRNVVGICAMDAKARSK------PCRNILNRIIAEG--EFEAIVFGDNTpVIP1 1 ------------------ASVAKRIRLGVCAMDKKARSK------PMSEILNRLNPE---TFEPVFFGDAUmVIP1 1 ---------------------MERIQLGVAAMDRKARSK------PMQNILNRLISTK--EFDVTIFGEKVCVIP1 1 ----MRRLLKLFRAATDGHANLPKIRIGVCAMDKKARSK------PMKEILERLTAWG--EFEVVIFGDDAtVIP2 1 ------------MGVEEGAGVDKKITIGVCVMEKKVFS------APMGQIMDRIHAFG--EFEIIHFGDKCrVIP2 210 SITSSSGSGSAFSNSATPGGFAGRLRLGVCAMARKADSK------PMREILRRLNTFN--EFELVMFPEEHsVIP2 20 NYRHFFHHADEDDEEEDDSPPERQIVVGICSMAKKSKSK------PMKEILERISLFK--YITVVVFEEEOsVIP2 1 -------------MVVEGNGRADSITIGVCVMEKKVFCS------PMEQILERLRAFG--EFEIIIFGDKVcVIP2 211 TTPGGGGGGGSGSRGVNMGSGPARIRLGVCAMARKAKSR------PMREILSRLTTCN--EFELVVFEEE

AnVIP1 151 VILDEAVENWPVCDFLIAFFSDGFPLDKAIAYARLRRPFCVNDLPMQKILWDRRLCLRILDHMSVPTPKRAtVIP1 58 VILEDPIESWPICDCLIAFHSSGYPLEKAQAYAALRKPFLVNELDPQYLLHDRRKVYEHLEMYGIPVPRYCeVIP1 57 VIINEPVENWPLCHCLVSFHSTEFPLEKAIAYVKLRNPYVINNLDRQYDLLDRRTVFKILSDNGIEHPRHCrVIP1 60 VICEKPTEEWPHVECMLCWHSDGFPLKKAQEYILNRRPFLVNDVFMQDALLDRRRVYKMLVEKNIPVPTHCsVIP1 65 TILHKPITEWPTCDCLLSWHSDGFPLPKAQKYAALRKPYLINDMVMQDVLLDRRRVYGSLQENGIPVPHHDmVIP1 105 VILREPVQNWPTCDCLVSFHSKGFPLEKAIEYAQLRNPFVLNNLHMQYDIQDRRRVYAILEKEGIEIPRYHsVIP1 93 VILNEPVENWPSCHCLISFHSKGFPLDKAVAYSKLRNPFLINDLAMQYYIQDRREVYRILQEEGIDLPRYMpVIP1 46 CILNQPIEQWPKVDALISFFSDRFPLAKVQAYAQLRKPFVVNDLDKQWDLLDRRKVYRTLAENDIPVPNHOlVIP1 67 CVVNEPVENWPKVDALIAFYSNGFPLQKVERYVEMHKPFVVNEPNDQWTLLDRRLVYKRLQEHDIPVPNHOsVIP1 52 IILEDPIESWPLCDCLIAFYSAGYPLEKAEKYAALRRPFLVNELDPQYLLHDRSKVYEHLKLFGVPVPTYOtVIP1 44 RIVNDPVETWPRVDALVSFYSNGFPLQKVEAYVELVKPFVVNDPHDQWTLLDRRLVYKRLQEHNIPVPNHPtVIP1 177 VITNKPIEEWPVCDVVVAFFSKGYPLDKAKEYVKLRKPFILNDLDTQELLQDRRRVYDLLEGSGIDVPRHScVIP1 226 VILDERIENWPTCDFLISFFSSGFPLDKAIKYVKLRKPFIINDLIMQKILWDRRLCLQVLEAYNVPTPPRSPVIP1 71 MILDEAVENWPACDYLICFYSSGFPLKKAEKYVELRKPFCVNDVVFQELLWDRRLVLNILDAIRVSTPQRTpVIP1 44 VILNEPVENWPVCDVLIAFYSNGYPLEKAEKYVTLRQPYLLNDLKMQRVLMDRRRVYDLLEESGIDVPRHUmVIP1 42 VILDEPVQDWPIVDVLISFFSTGFPLDKAISYADLRKPVLVNDLRLQQVLWDRRAVLSILDSVGVPTPRRVCVIP1 59 AILEKPIEDWPHVECLLCWHSDGFPLKKAQEYIKYRRPFLVNDVFMQDFLLDRRKVYKLLVERSIPVPTHAtVIP2 51 VILEDPVESWPICDCLIAFYSSGYPLEKVQAYSSLRKPFLVNELDPQYLLHDRRKVYEHLEMYGIPVPRYCrVIP2 272 VILGQPASEWPVVDCLMAWHSDGFPLSRAQEYVALRRPFCVNDLGAQELLLDRRRVYRLLQDSCIPVPRHHsVIP2 82 VILNEPVENWPLCDCLISFHSKGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRREVYSILQAEGILLPRYOsVIP2 50 VILEDPIEIWPKCDCLIAFYSSGFPLKKAEAYAALRRPFLVNELEPQHLLHDRRKVYEHLEKYGIPVPNYVcVIP2 273 VILQQPVDRWPVVDCLLSWYSEAFPLRKAQEYVERVRPFCVNDLAAQEVLLDRRKVYRLLQDSSIPVPRH

AnVIP1 221 IEVNRDGGPTLESPELAQHVYKLTGVKLDGPTDGTGGGTPKTKNVTLSDDGDSLIVDGKHFRKPFVEKPVAtVIP1 128 ACVNRKVPNQD--------------------------------LHYFVEEEDFVEVHGERFWKPFVEKPVCeVIP1 127 GYVIRGRPNEPD--------------------------------TELVEHPDHIEVNGEVFNKPFVEKPICrVIP1 130 IIVERDGLPEGV-----------------------------TDPPGFVEDEDYVELNGQRIYKPFVEKPICsVIP1 135 ICVDRDNLPPG------------------------------TDPPGFVETEDYVEQNGVQIKKPFVEKPVDmVIP1 175 AVLDRDSPDPKH--------------------------------HELIESEDHVEVNGITFNKPFVEKPVHsVIP1 163 AVLNRDPARPEE--------------------------------CNLIEGEDQVEVNGAVFPKPFVEKPVMpVIP1 116 IVINRNEEVKPG---------------------VMPDWSRDFEAPGFEEHEDYVELDGKRIDKPFVEKPAOlVIP1 137 VVVN-----------------------------LALPDQPTFEPKNFSQDEEYVEINGKRIYKPFVEKPAOsVIP1 122 AVVRREYPNQE--------------------------------LKYFVEQDDFIEIHGKRFYKPFVEKPIOtVIP1 114 IVVNRNDYVKRGGL-----------RKKPKGDEVALPDQPTFEPKEFSQDEEYVEIQGKRICKP------PtVIP1 247 VYLSRDGYIST----------------------------------GTGDGNGSRHQEVIEFDDHIERNPSScVIP1 296 LEISRDGGPRANEELRAKLR-EHGVEVKPVEEP-----------EWKMVDDDTLEVDGKTMTKPFVEKPVSPVIP1 141 LICSRDGGPKINKVLEEKLRRKFGIEITEVPTP-----------EVKMLDEDTLSVDGKIIKKPYVEKPVTpVIP1 114 VFMSRDGYEPD----------------------------------IEEHDDHIEVNGVVIHKPFVEK-PIUmVIP1 112 LEVDRDGGPDLEDVILQDLNKRLGVDLKKDRQP----------KPCKLDGYDHLIIGTNRISKPFVEKPVVCVIP1 129 IIVERDHLPEGT-----------------------------TDPPGFVEDEDYVELNGQRIVKPFVEKPVAtVIP2 121 ACVNRKVPDED--------------------------------LDYFVEEEDFVEVKGERFWKPFVEKPVCrVIP2 342 VVVSREGLAPG------------------------------QSPPGFVEGEDFVEMDGVRIAKPFVEKPAHsVIP2 152 AILNRDPNNPKE--------------------------------CNLIEGEDHVEVNGEVFQKPFVEKPVOsVIP2 120 ALVNREYPYQE--------------------------------LDYFIEQEDFVEVHGKRFMKPFVEKPVVcVIP2 343 IVVNREGLEPG------------------------------TSPPGFVEGDEYIELDGERISKPFVEKPA

3

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 291 SGEDHNIHIYFPKDQQYGGGGRRLFRKVGNKSSEYDPDLRTPR------SILEDGSSYIYEQFLRVDNAEAtVIP1 166 NGDDHSIMIYYPS--SAGGGMKELFRKIGNRSSEFHPDVR----------RVRREGSYIYEEFMATG-GTCeVIP1 165 SSEDHNVYIYYPS--SVGGGSQRLFRKINNRSSWYSPKSE-----------VRKEGSYIYEEFIPAD-GTCrVIP1 171 SGEDHNIWVYYPH--SMGGGVKYLFRKVDDKASKYDSEHN---------GCVRRDGSFIYEEFLPTG-GTCsVIP1 175 SGEDHNIYIYYPH--AMGGGVKRLYRKIENRSGDYDPNHP---------GTVRRDGSYIIEEFLTTG-GTDmVIP1 213 SAEDHNIYIYYPT--SAGGGSQRLFRKIGSRSSVYSPESR-----------VRKTGSFIYEDFMPTD-GTHsVIP1 201 SAEDHNVYIYYPS--SAGGGSQRLFRKIGSRSSVYSPESS-----------VRKTGSYIYEEFMPTD-GTMpVIP1 165 DAENHNICIYYPH--TVGGGYKALFRKVGNQASRYYPPP--APDSSKPYTPVRRDTSFIYEDFMSTG-GTOlVIP1 178 NAEDHNIFIYYPH--SVGGGYKRLFRKIGNQSSQYYPPPETTAAGELSYAPVRETTSFIYEDFMSTN-GTOsVIP1 160 DGDDHNIMIYYPS--SAGGGMKELFRKVGNRSSEFYPDIR----------KVRRDGSYIYEEFMPTG-GTOtVIP1 167 -----NIFIYYPH--SVGGGYKRLFRKIGNQSSQYYPPPEPVGDGLP-YTPVRETTSFIYEDFMSTN-GTPtVIP1 283 MQKTTILPFSYPT--SAGGGCKKLFRKIGNRSSEFYPDIN----------EVRRDGSYIYEEFVETQ-GTScVIP1 354 DGEDHNIYIYYHS--KNGGGGRRLFRKVGNKSSEFDPTLVHPR------TEG----SYIYEQFMDTDNFESPVIP1 200 YGEDHNIYIYFPK--SVGGGGRKLFRKVANKSSDYDPDLCAPR------TEG----SFIYEEFMNVDNAETpVIP1 149 DADDHNIAIYYPS--SAGGGCKKLFRKVGDRSSEFYPEIN----------EIRRDGSYIYEEFIETQ-GTUmVIP1 172 SGEDHNIHIYFAQ--NRGGGGRRLFRKVGNKSSEYDPNLVEPR------TDG----SYIYEEFMDVDNAEVCVIP1 170 SGEDHNIWVYYPH--SMGGGVKYLFRKVDDKASKYDSSHD---------GKVRRDGSYIYEEFLPTG-GTAtVIP2 159 NGDDHSIMIYYPS--SAGGGMKELFRKVGNRSSEFHPDVR----------RVRREGSYIYEEFMPTG-GTCrVIP2 382 SGEDHNIHIYYPA--AMGGGVKRLFRKVANRSGAYDPAHS---------GAVRREGSFIYEEFLATG-GTHsVIP2 190 SAEDHNVYIYYPT--SAGGGSQRLFRKIGSRSSVYSPESN-----------VRKTGSYIYEEFMPTD-GTOsVIP2 158 NGDDHRIMIYYPN--SAGGGMKELFRKVGNRSSEFHPDVR----------RVRREGSYIYEEFMPTG-GTVcVIP2 383 SGEDHNIWVYYPT--SMGGGVKRLFRKVADRSGNYDPGHS---------GAVRRDGSYIYEEFLATG-GT

AnVIP1 355 DVKAYTVGPDFCHAETRKSPVVDGLVRRNTHGKELRYITKLSKEE-AILRDIFINERRRRESFSLLKSPGAtVIP1 223 DVKVYTVGPEYAHAEARKSPVVDGVVMRNTDGKEVRYPVLLTPAEKQMAREVCIAFRQAVCGFDLLRSEGCeVIP1 221 DVKVYAVGPFYAHAEARKAPGLDGKVERDSDGKEVRYPVILSNKEKQIAKKIVLAFGQTVCGFDLLRANGCrVIP1 229 DVKVYTVGPRYAHAEARKSPVVDGKVLRSADGKEMRFPVLLSPQEKEIARMVCLAFGQKVCGFDLLRSEKCsVIP1 233 DVKVYTVGPRYAHAEARKSPVVDGKVTRSADGKELRFPVLLSPQEKEIARMVCLAFGQKVCGFDLLRSERDmVIP1 269 DVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEIRYPVILNHSEKLISRKVCLAFKQTVCGFDLLRANGHsVIP1 257 DVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEIRYPVMLTAMEKLVARKVCVAFKQTVCGFDLLRANGMpVIP1 230 DVKVYTVGPNYAHAEARKSPVVDGRVQRDENGKEERFPVLLTPDEKEIARRVCLAFGQTVCGFDLLR-TKOlVIP1 245 DVKVYTVGPNYAHAEARKSPVVDGRVQRDESGKEVRYPVLLTPEEKEIARRVCIAFGQRVCGFDLLR-AKOsVIP1 217 DVKVYTVGPGYAHAEARKSPVVDGVVMRNSDGKEVRYPVLLTPTEKQIARNICQAFGQAVCGFDLLRCDLOtVIP1 228 DVKVYTVGPNYAHAEARKSPVVDGRVQRDESGKEVRYPILLTPEEKEIARRVVLAFGQNVCGFDLLR-AKPtVIP1 340 DVKMYTVGPEYGHAEARKSPAVDGKVERNSDGKEVRFPVILTYREKETARRIVLVFKQFVCGFDILRVQVScVIP1 412 DVKAYTIGENFCHAETRKSPVVDGIVRRNTHGKEVRYITELSDEEKTIAGKVSKAFSQMICGFDLLRVSGSPVIP1 258 DVKVYTVGPHYSHAETRKSPVVDGIVRRNPHGKEIRFITNLSEEEKNMASKISIAFEQPVCGFDLLRVSGTpVIP1 206 DVKMYTVGPDYGHAEARKSPAVDGKVERNPDGKEVRFPVILTLREKEIARRIVLVFKQQVCGFDILRIQEUmVIP1 230 DIKVYTIGPHFVHAETRKSPVVDGVVKRNPDGKEIRYITKLSDQEIKMATSISMAFKQNICGFDLLRVGGVCVIP1 228 DVKVYTVGPRYAHAEARKSPVVDGKVLRNADGKEMRFPVLLSPQEKEIARMVCLAFGQKVCGFDLLRSEKAtVIP2 216 DVKVYTVGPEYAHAEARKSPVVDGVVMRNPDGKEVRYPVLLTPAEKQMAREVCIAFRQAVCGFDLLRSEGCrVIP2 440 DVKVYTVGPRYAHAEARKSPVVDGRVARSADGKEVRFPVVLSPQEKEVARMVCLAFGQKICGFDLLRSERHsVIP2 246 DVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGOsVIP2 215 DVKVYTVGPGYAHAEARKSPVVDGVVMRNPDGKEVRYPVLLTPTEKQMSRDVCSAFRQMVCGFDLLRCDGVcVIP2 441 DVKVYTVGPRYAHAEARKSPVVDGRVARTADGKEVRFPVLLSPQEKEIARMVCLAFGQKVCGFDLLRSER

AnVIP1 424 SS-----KSSDVGSLESSHDGMDFNNGRAGVIPKEQSASPGICTPQGANQPSPTMH--------------AtVIP1 293 -----CSYVCDVNGWSFVKNSYKYYDDAACVLRKMCLDAKAPHLSSTLPPTLPWKV--------------CeVIP1 291 KS-----YVCDVNGFSFVKTSTKYYEDTAKILGNQIVRHYAKSKNWRVPSDMPQPP--------------CrVIP1 299 GR----SYVCDVNGWSFVKNSKKYYDDAAGILRSIILSALAPHRLNVQPHLPTHS---------------CsVIP1 303 GK----SYVCDVNGWSFVKNSHKYYDDAAGILRSIILSAIAPHRLATAPPQLPMS---------------DmVIP1 339 KS-----YVCDVNGFSFVKNSNKYYDDCAKILGNMILRELTP---------TLHIP--------------HsVIP1 327 HS-----FVCDVNGFSFVKNSMKYYDDCAKILGNTIMRELAP---------QFQIP--------------MpVIP1 299 GR----SYVCDVNGWSFVKNSTKYFDDASVCLRAIILQAVAPEHKATMEATEAADQ--------------OlVIP1 314 GR----SYVCDVNGWSFVKNSKKYYDDASVCLRAMILKAVAPNHFSTQPAQKAAAS--------------OsVIP1 287 GE--ARSYVCDVNGWSFVKSSHKYYDDAACILRKMFLDDKAPHISSTIPANLPWKV--------------OtVIP1 297 GR----SYVCDVNGWSFVKNSKKYYDDASVCLRAMILKAVAPNHLSTKPAQKAAAS--------------PtVIP1 410 GQSV-VS---------------KYYDDCAQILSEHILALIKPAALKTFSTLDPLVTTSLNRLDEEITEGDScVIP1 482 KS-----YVIDVNGFSFVKDNKAYYDSCANILRSTFIEAKKKMDMEKKNLP-------------------SPVIP1 328 QS-----YVIDVNGWSFVKDNNDYYDNAARILKQMFHVAERHRRNRVPSVQ-------------------TpVIP1 276 GDSL-VSYVCDVNGWSFVKKSRKYYDDCAQILTEHMLATLKPKSKISLSTLAPLLATMEDSVDDMN----UmVIP1 300 KS-----YVIDVNGWSFVKGNDFYYDKCAEILSRFCKNNVVRRPIASSTSG-------------------VCVIP1 298 GR----SYVCDVNGWSFVKNSKKYYDDAADILRSIILSALAPHRLNVQPNLPTYT---------------AtVIP2 286 -----SSYVCDVNGWSFVKNSYKYYDDAACVLRKMFLDAKAPHLSSTIPPILPWKI--------------CrVIP2 510 GG----SYVCDVNGLSLVKNSTKFYADAADILRSILLQALAPHRLHLMPALPATPTFGLMLES-------HsVIP2 316 QS-----YVCDVNGFSFVKNSMKYYDDCAKILGNIVMRELAP---------QFHIP--------------OsVIP2 285 -----RSYVCDVNGWSFVKNSYKYYDDAACILRKIFLDAKAPHLSSTIPPSLPWKS--------------VcVIP2 511 GG----SYVCDVNGWSFVKSSAKFFGDAADILRSIILQALAPHRLHLVPALPPAPTFGWVPIQALLQPPK

4

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 475 ----------------------------------------------------------------------AtVIP1 344 ----------------------------------------------------------------------CeVIP1 342 ----------------------------------------------------------------------CrVIP1 350 ----------------------------------------------------------------------CsVIP1 354 ----------------------------------------------------------------------DmVIP1 381 ----------------------------------------------------------------------HsVIP1 369 ----------------------------------------------------------------------MpVIP1 351 ----------------------------------------------------------------------OlVIP1 366 ----------------------------------------------------------------------OsVIP1 341 ----------------------------------------------------------------------OtVIP1 349 ----------------------------------------------------------------------PtVIP1 464 VPKRKDTFMSRASRMLQGEPAEDDDEEEDSKYKALLPHENTHSPPRSETGELLSNAATTLVEGAVPVREFScVIP1 528 ----------------------------------------------------------------------SPVIP1 374 ----------------------------------------------------------------------TpVIP1 341 ----------------------------------------------------------------------UmVIP1 346 ----------------------------------------------------------------------VCVIP1 349 ----------------------------------------------------------------------AtVIP2 337 ----------------------------------------------------------------------CrVIP2 569 -------------------------QRTMAAAEAAADAMAAATAAAVAGGGAAAAAAAVAAAGDGGSSIPHsVIP2 358 ----------------------------------------------------------------------OsVIP2 336 ----------------------------------------------------------------------VcVIP2 577 TSGHHHSLLQPTHGL----------MRESQQALAAAEAAAAAAAAVAATGCCSSLAGPEGLLRQDTFIPT

AnVIP1 475 -----------------------------SLEANPPPPASKHSWKLKGMVAVIRHADRTPKQKFKFTFHSAtVIP1 344 -------------------NEPVQSNEGLTRQGSGIIGTFGQSEELRCVIAVVRHGDRTPKQKVKLKVTECeVIP1 342 -----------------------ILDLGLGDDPPMITTPSGKLAELRCVVAVIRHGDRTPKQKMKLIVTDCrVIP1 350 ---SATNPDTGSAVVSDEEDDDDALVPGMDVSEVRHTAKRSQKEELRCVLAVVRHGDRTPKQKLKVVVTQCsVIP1 354 ---SANSAKDDAMMLAERQATEEPNFLPYAAMDSSDEVDDRHYEELRCVLAIVRHGDRTPKQKMKMKITQDmVIP1 381 -----------------------WSVPFQLDDPPIVPTTFGKMMELRCVVAVIRHGDRTPKQKMKVEVRHHsVIP1 369 -----------------------WSIPTEAEDIPIVPTTSGTMMELRCVIAIIRHGDRTPKQKMKMEVKHMpVIP1 351 -------TTTEDDPNAEAMQQQPDESPAEQKKKGKKGQPEPPKEELRAVLAVIRHGDRTPKQKMKMRVKHOlVIP1 366 -------ASVEE-PDIILD---GNDAELKETRLEKAPSPKEPPEELRAVLGVIRHGDRTPKQKMKLRVKNOsVIP1 341 -------------------SEPVQPFDAVRDRERGTVGISRQSEELRCVIAVIRHGDRTPKQKVKLKVTEOtVIP1 349 -------RADEP-DVISTDGFTEAELNDFIPERVPTPTPSEPAEELRAVLGVIRHGDRTPKQKMKLRVTNPtVIP1 534 PNNLTSEPASLTHSANSSVVDEKDRDTPRRRRSTSLSMPGSHQEELRCVLAIVRHGDRTPKQKLKVNMTEScVIP1 528 -------------------------------IIREE---KEQKWVFKGLAIIIRHADRTPKQKFKHSFTSSPVIP1 374 -------------------------------EVLNPPPRESEAWRLKSLVGVLRHADRTPKQKFKFSFTSTpVIP1 341 ------------------------------RRRGRDFVPSTHQEELRCVITIIRHGDRTPKQKLKGDINGUmVIP1 346 ------------------------------LASASPRERERSAWNLKASVTVFRHGDRTPKQKLKRSFKPVCVIP1 349 ---GALTNPDTGSNLASDDEDDESLVPGMDVSEVRHTAKRMQKEELRCVLAVIRHGDRTPKQKLKVVVTQAtVIP2 337 -------------------NEPVQSNEGLTRQGSGIIGTFGQSEELRCVIAIVRHGDRTPKQKVKLKVTECrVIP2 614 DGGVELIRAPTWLRPGGGGEGSSSSGGGGGVSGGGSSGGGLPGEELRCVLAVVRHGDRTPKQKVKLEVSHHsVIP2 358 -----------------------WSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHOsVIP2 336 -------------------NEPVQPTEGLTRQGSGIIGTFGQSEELRSVIVVIRHGDRTPKQKVKLKVTEVcVIP2 637 MAAAAAAAARLGSLDGGPASPCSPPLTPAAAAAAAAAGWGRPGEELRCVLAVIRHGDRTPKQKVKVVVNH

AnVIP1 516 -----QPFIDLLKGHQEE---------VVIKGESALRSVSEAVNLAMEQGLEDADKLKLLRTSLE-----AtVIP1 395 EK-----LLNLMLKYNGG----KPRAETKLKSAVQLQDLLDATRMLVPRTRPGRESDSDAEDLEHAEKLRCeVIP1 389 QR-----FFALFEKYDGYK-----KHEIKMKKPNQLMEVLELARALVIEKQRDRHQILEKLREGTGEEEICrVIP1 417 EP-----LLALFHKYKD-----AKGKQAKLKSPLQLQELLDITRQLVKDFERRDDEK--EGG--VPPLGDCsVIP1 421 EP-----LLELLRKHMD-----SKGKQAKLKSPAELQELLDVTRALLADMDKNRSAKGADGDGAVATGELDmVIP1 428 PK-----FFEIFEKYDGYK-----LGHVKLKRPKQLQEILDIARFLLSEIHTKAHAEIEEKES-------HsVIP1 416 PR-----FFALFEKHGGYK-----TGKLKLKRPEQLQEVLDITRLLLAELEKEPGGEIEEKTG-------MpVIP1 414 EP-----LLQLLKKCTGD----RPRKQAKLKTPARLQELLNISRVLDSPPLAQPRS--SCGSKEEWEEEVOlVIP1 425 RE-----LLDLMLRCTNG----RTRKQAKLKTPQTLQELLNICRDIYKSLSKQTLAPKDEEGHEEAQEELOsVIP1 392 EK-----LLKLMLKYNGG----KAHAEAKLKSALQLQDLLDATRILVPRARSGRESDS-DAEIEHAEKLROtVIP1 411 PE-----LLSLMLRCTNG----RTRKQAKLKTPQLLQELLDICRDIYAGLAERSSQ-ADITLSEEALEELPtVIP1 604 PH-----ILKYFHDHNKGD----CQKDLKVKAKAPLTEFLQTVKNTLKELPGTDHEGVR---YQMMHMR-ScVIP1 564 -----PIFISLLKGHKEE---------VVIRNVNDLKIVLQALRIALDEKAGNPAKIKVLANALE-----SPVIP1 413 -----DPFVKLLQGHTEE---------VILRNE-QLNSVLAATNLATELKCEDINKLKQLRLALE-----TpVIP1 381 KR-----FLEYFQGHAKN-----VKKDLKVKAKQPMVEFLETVKAVIKDKEEEGAKKNREELYKARHIR-UmVIP1 386 GDTWTAPLIALLQGRRQE---------IILRTQLDLVSSAASEALALPG--ANVQDLELIIQLID-----VCVIP1 416 EP-----LLNLFHKYKD-----AKGKQAKLKSPLQMQELLDITRQLVKDFERRDDEK--EGGPGAGALNDAtVIP2 388 EK-----LLNLMLKYNGG----KPRAETKLKTAVQLQDLLDATRMLIPRARSG-ESDSDAEDLEHADKLRCrVIP2 684 P-----AFLALYQRHVDPSKPPGPHRQAKLKSASQLQELLDVTRQLLQQHPQLQPQPHPQPQPHNRPSSGHsVIP2 405 QK-----FFDLFEKCDGYK-----SGKLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKP-------OsVIP2 387 EK-----LLNLMLKYNGG----KPRAETKLKSAVQLQDLLDATRQLVPPTRSGRESDSDAEDLEHIEKLRVcVIP2 707 TGAPSPAFLELYERYADPGKRPGLGRQAKLKSASQLQELLDVTRRLLQR-----PEATTASTASTATIAA

5

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 567 ----------------------------------------------------------------------AtVIP1 456 QVK-------------------------------------------------------------------CeVIP1 449 HKSEHDLE----------------------------------------------------VCEEEMKKWECrVIP1 473 EAKEAAQ--------------------------------------------------------EVRGKLRCsVIP1 481 KLDADQD--------------------------------------------------------ELREKFGDmVIP1 481 -------------------------------------------------------------------KLEHsVIP1 469 -------------------------------------------------------------------KLEMpVIP1 473 EQWKQVVS--------------------------------------------------------------OlVIP1 486 EAWKQVVS--------------------------------------------------------------OsVIP1 452 QVR-------------------------------------------------------------------OtVIP1 471 EAWKQIVS--------------------------------------------------------------PtVIP1 661 ----------------------------------------------------------------------ScVIP1 615 ----------------------------------------------------------------------SPVIP1 463 ----------------------------------------------------------------------TpVIP1 440 ----------------------------------------------------------------------UmVIP1 440 ----------------------------------------------------------------------VCVIP1 474 EEREAAQ--------------------------------------------------------EVRGKLRAtVIP2 448 QVK-------------------------------------------------------------------CrVIP2 749 GGGGGSGAASDGGAGATPAPKAGDLPAGQQKAAAAAAAAAAAAAAPAAAGQQQQPQQPPLVDADTLAVLAHsVIP2 458 -------------------------------------------------------------------KLEOsVIP2 448 QVK-------------------------------------------------------------------VcVIP2 772 AGEGSGSGSGDVGAVMLPSYPMAAVAGCEGAGSSGAAAAAAEVVSTAAAEVD-------LVAAETRSALA

AnVIP1 567 ---------KKGGWPGTKVQIKPMFRRRDLEGGNVKTSALSDTVEKEPNSLPIDAPQEGENIGRSQTRSDAtVIP1 459 ---AVLEEGGHFSGIYRKVQLKPLKWVKIPKSDGDGEEE-------------------------------CeVIP1 467 QMRTVLEMYGHFSGINRKVQMKYLKERETKTSDEELR---------------------------------CrVIP1 487 IMQTVLESGGQFSGINRKVQIKPLRWGVAPPAASASDGVASPTAAAAPVAHAGPIAGAGGSMEMLTGLGSCsVIP1 495 IVRTVLEHGGSFAGVNRKVQLKPLRWAPPEEGSQD-----------------------------------DmVIP1 484 QLKNVLEMYGHFSGINRKVQMKYQPKGRPRGSSSDDTNL-------------------------------HsVIP1 472 QLKSVLEMYGHFSGINRKVQLTYYPHGVKASNEGQD----------------------------------MpVIP1 481 ----ILQEGGHFSGINRKAQLKPLAWEEIPEDQRKPTKDGKEGPTERVTQA-------------------OlVIP1 494 ----ILEEGGHFSGINRKAQLKPLEWETIDAGTLDSH-------TEKVTEA-------------------OsVIP1 455 ---AVLEEGGHFSGIYRKVQLKPSNWVHIPKSNGNGKEE-------------------------------OtVIP1 479 ----ILEEGGHFSGINRKAQLKPLEWEKVEG-ENDVQ-------NEKVVEA-------------------PtVIP1 661 ----DILERWKILGLNRKLQIKPRKFEEYEDEKGELKKR-------------------------------ScVIP1 615 ---------KKLNFPGTKIQLKPVLNKENE----------------------------------------SPVIP1 463 ---------TKKDLPGTKVQLKPAYSPEGK----------------------------------------TpVIP1 440 ----DILLRWKFSGLNRKLQMKPRKWIEEETPDGNKVTK-------------------------------UmVIP1 440 ---------RKKDLPGTKVQIKPSFDSDTC----------------------------------------VCVIP1 488 IMQTVLESGGQFSGINRKVQIKPLRWGIAADGLVG-----VAGATAAGGGGGGSGSIQSAAMHSNGGGGNAtVIP2 451 ---AVLEEGGHFSGIYRKVQLKPLKWVNVPKSDGEGEEE-------------------------------CrVIP2 819 VVRGVLEAGGRFSGVNRKAQLKPRGWVTAAAAAAGEAAPPPSPPPATAVATGVGVGHVDERLLQGRQRGDHsVIP2 461 QLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEE----------------------------------OsVIP2 451 ---AVLEEGGHFSGIYRKVQLKPLKWIRVPKRCGDGEEE-------------------------------VcVIP2 835 VVRSVLEAGGHFSGVNRKAQLKPRTWVEPQQPTPPPPPQPAPPTPTNTAVTAAPLAVAMS----------

AnVIP1 628 SISG------------------------------------------------------------------AtVIP1 495 ----------------------------------------------------------------------CeVIP1 504 ----------------------------------------------------------------------CrVIP1 557 GIDVGSSSMATLHNSPSVGGAVNASSSAGQPVLEEG----------------------------------CsVIP1 530 -----------------------------APRIVEA----------------------------------DmVIP1 523 ----------------------------------------------------------------------HsVIP1 508 ----------------------------------------------------------------------MpVIP1 528 ----------------------------------------------------------------------OlVIP1 534 ----------------------------------------------------------------------OsVIP1 491 ----------------------------------------------------------------------OtVIP1 518 ----------------------------------------------------------------------PtVIP1 696 ----------------------------------------------------------------------ScVIP1 636 ----------------------------------------------------------------------SPVIP1 484 ----------------------------------------------------------------------TpVIP1 475 ----------------------------------------------------------------------UmVIP1 461 ----------------------------------------------------------------------VCVIP1 553 GSSQNFEVASGSVDMLTTLSSTTPGGGAPAPVLEEA----------------------------------AtVIP2 487 ----------------------------------------------------------------------CrVIP2 889 SPAALGWASGSSSSAAVTPAASRVGSMTPPPPAPPASDSATATISASDAATASATASATASGPAAATSPAHsVIP2 497 ----------------------------------------------------------------------OsVIP2 487 ----------------------------------------------------------------------VcVIP2 895 -----GSGAGSSSSSSSSGGGEGTQLHPHPHPHPHARYRPLAGPTATPTATPTATPTVKSS---------

6

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 632 ---ATFSRFSAVENDLILDKLQLVIKWGGEPTHAARYQSQDLGLNMRDDLKLMNK---------------AtVIP1 495 ----------------RPVEALMVLKYGGVLTHAGRKQAEELGRYFRNNMYPGEG------------TGLCeVIP1 504 ---------------REGPALLLILKWGGELTTAGNMQAEALGRLFRTLYPGIRRTDGKSSPEDTQGLGFCrVIP1 593 ---------------------LLILKWGGVLTHAGRQQAEDLGKIYRMVMYPSG------------GNGLCsVIP1 537 ---------------------LLILKHGGVLTHAGRQQAEALGNVFRTVMYPRYG---------PAGGGLDmVIP1 523 -----------AADQPVEPSLVLILKWGGELTPAGRIQAEELGRIFRCMYPGGQGR---SDYSGTQGLGLHsVIP1 508 -----------PQRETLAPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQG-----DYAGFPGCGLMpVIP1 528 ---------------------LLILKFGGVLTHLGKNQAEFLGRDFRMRMYPGG---------DPNSDGLOlVIP1 534 ---------------------LLIIKFGGVLTYLGKYQAETLGKAFRVRMYPRGNY------YGSDADGLOsVIP1 491 ----------------YPIEALMVLKYGGVLTHAGRKQAEELGRYFRNNMYPSEG------------PGLOtVIP1 518 ---------------------LLIIKFGGVLTYLGKYQAEALGKAFRVRMYPRGNY------YGSDADGLPtVIP1 696 -----------------CVEVQLILKWGGNLTKLGEKQSINLGRRLRHEMYPDAP-----------GGGIScVIP1 636 -----------------VEKVQFILKWGGEPTHSAKYQATELGEQMRQDFDLLNK---------------SPVIP1 484 -----------------LLKLQLIIKWGGEFTHSARYQSKDLGEQFHKDLYIMNR---------------TpVIP1 475 -----------------CSELQMIIKWGGDLTKLGEKQAVNLGNRLRNELYPESG-----------GGGIUmVIP1 461 ----------------ELAKMQLIIKWGGEFSHAARHQAKELGNNMRKDMIIMNA---------------VCVIP1 589 ---------------------LLILKWGGVLTHAGRQQAEDLGKIYRMVMYPSG------------GNGLAtVIP2 487 ----------------RPVEALMVLKYGGVLTHAGRKQAEELGRYFRNNMYPGEG------------TGLCrVIP2 959 AATAATAVAGGGRPGGGPSSLLLIVKWGGVLTHAGRAQAEMLGVRFRCGMYPRAG---------PAGGGLHsVIP2 497 -----------DSR-REEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQG-----DYAGFPGCGLOsVIP2 487 ----------------RPIEALMILKYGGVLTHAGRKQAEELGRFFRNNIYPGEG------------TGLVcVIP2 951 --------------SGGPGSLLLIVKWGGVLTHAGRAQAETLGVRFRTGMYPRAG---------PAGGGL

AnVIP1 684 -----EALNNVRVFTSSER---------------RKEIPEGFIQVRKDLLDDS----------NAAKDLMAtVIP1 537 LRLHSTYRHDLKIYSSDEGRVQMSAAAFAKGLLDLEG---QLTPILVSLVSKD--SSMLDGLDN-ASIEMCeVIP1 559 LRLHSTYRHDLKIYASDEGRVQTTAAAFAKGLLALEG---ELTPILMQMVKSANTDGLLDDDCQ-ARLYQCrVIP1 630 LRLHSTYRHDLKIYSSDEGRVQTSAAAFTKAMLDLEG--ASLTPILVSLVNKD--ASMLEAFGKGASDDICsVIP1 577 LRLHSTYRHDLKIYSSDEGRVQTSAAAFTQGLLDLEG--DSLTPILVSLVKKD--AGMLDAFGKGASEDIDmVIP1 579 LRLHSTFRHDLKIYASDEGRVQMTAAAFAKGLLALEG---ELTPILVQMVKSANTNGLLDNDCD-SSKYQHsVIP1 562 LRLHSTFRHDLKIYASDEGRVQMTAAAFAKGLLALEG---ELTPILVQMVKSANMNGLLDSDGDSLSSCQMpVIP1 568 LRLHSTYRHDLKIYSSDEGRVQITAAAFIKGLLDLETENNQLTPILASLVNKD--AKLLDFVTHEVEEDIOlVIP1 577 LRLHSTYRHDLKIYSSDEGRVQITAAAFAKGLLALDTHHGQLTPILASLVTKD--AKLLDFVTHYVEEDIOsVIP1 533 LRLHSTYRHDLKIYSSDEGRVQMSAAAFAKGLLDLEG---ELTPILVSLVSKD--SSMLDGLQD-GSIEIOtVIP1 561 LRLHSTYRHDLKIYSSDEGRVQITAAAFAKGLLALDTHHGQLTPILASLVTKD--AKLLDFVTHDVEEDIPtVIP1 738 LRLHSTFRHDLKIKTSDEGRVMKTAAAFAKGLLELEG---DLPPILVSLVHKE--KGSLHMLDPSGNKEVScVIP1 674 -----SILQNIKIFSSSERRVLHTAQYWTRALFGADELGSDEISIRKDLLDDS----------NAAKDLMSPVIP1 522 -----DCLKDVEIYTSSERRVSASAEIFAMAFLEQETIPSDLLKVRKDLLDDS----------NAAKDTMTpVIP1 517 LRLHSTFRHDLKIKTSDEGRVMKTAAAFAKGMLELEG---DIPPILVSLVHKE--KSSGHMLDPSGNKEVUmVIP1 500 -----DALSNCTVYTSSERRVTASAEIFAAAFLDESSG-EKEMIIRKDLLDDS----------NAAKDVMVCVIP1 626 LRLHSTYRHDLKIYSSDEGRVQTSAAAFTKAMLDLEG--NSLTPILVSLVNKD--ASMLEAFGKGASDDIAtVIP2 529 LRLHSTYRHDLKIYSSDEGRVQMSAAAFAKGLLDLEG---QLTPILVSLVSKD--SSMLDGLDN-ASSEMCrVIP2 1020 LRLHSTYRHDFKVYSSDEGRVQTSAAAFAQGLLSLEG--ASLTPITASLVNKD--ALLLEAYGKGASADMHsVIP2 550 LRLHSTYRHDLKIYASDEGRVQMTAAAFAKGLLALEG---ELTPILVQMVKSANMNGLLDSDSDSLSSCQOsVIP2 529 LRLHSTYRHDLKIYSSDEGRVQMSAAAFAKGLLDLEG---QLTPILVSLVSKD--SSMLDGLED-ASIEMVcVIP2 998 LRLHSTYRHDFKVYSSDEGRVQTSAAAFTQGLLCLEG--TSLTPILASLVNKD--AAMLEAFGKGASYDM

AnVIP1 724 DKVKKKLKLLLREGS--APSQFTWPKEN------------------------------------------AtVIP1 601 EAAKARLNEIVTSGTKMIDDDQVSSEDFPWMTDG------------------------------------CeVIP1 625 TELKRYLHKALQADRDFTPQDYLELNPNGLRAITAA----------------------------------CrVIP1 696 AEAKEALYQAMTWDPEKPGDKP-LAVSKLSHTPMISPPLSPKVSRASSFAAKSAGGVAAAAAAEVSSLQTCsVIP1 643 RAAKAELYAQMTWDP--MTSQS-LNTPPQITTPMPSPPPSPKTDSALHKANSGSMNSPPQGVSIFKAADADmVIP1 645 NLAKGRLHELMQNDREFSKEDRELINPCNSKSITQA----------------------------------HsVIP1 629 HRVKARLHHILQQDAPFGPEDYDQLAPTRSTSLLNS----------------------------------MpVIP1 636 LHAKQKLYNIMTDGHVKGKNKNKEYSTS------------------------------------------OlVIP1 645 LHSKHKLAGESSHHDTTQKAVTLSTN--------------------------------------------OsVIP1 597 DEAKARLHNIILSSKIANG----ETMEFPWMVDG------------------------------------OtVIP1 629 LHSKHKLYAAMTSKPDRRPSETETETGALNQTLRFASLNKFKIVTQMVIASHRIQSARDFSSS-------PtVIP1 803 KMELDECKEKITANLQRDIDIGTAKESEIEQLVGPM----------------------------------ScVIP1 729 DKVKKKLKPLLREGK--EAPPQFAWPSK------------------------------------------SPVIP1 577 DKVKKHLKSLLRVGD--TARKEFTWPEN------------------------------------------TpVIP1 582 KKDLEVSCTAIIITNLN--------------MVGPE----------------------------------UmVIP1 554 DVVKKKLKASLRPDSPEANQVPDDWPQD------------------------------------------VCVIP1 692 ADAKEALYQAMTWDPDKPSMAP-LAVSKRSHTPMISPPMSPKVSRTSSFATKGSGLSAAAAVAVAAAAAAAtVIP2 593 EAAKAQLNEIITAGSKMVHDHVSS--ELPWMTDG------------------------------------CrVIP2 1086 AAAKQELYARLTWDPEAGVSRCKPPGGSSSGSGSSSPAPPPAATALASMPSLATSTTSGSSSSQQTTPLPHsVIP2 617 QRVKARLHEILQKDRDFTAEDYEKLTPSGSISLIKS----------------------------------OsVIP2 593 DEAKARLHEIIITNAKAKN--TNEPVEFPWMVDG------------------------------------VcVIP2 1064 RAAKEELYKRLTWDPAARVSRCKLPAAPPPPSPAPTPPPSPKLRPASTPPHPPSAAATTTPTGPPRVTSL

7

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 750 ----------------------------------------------------------------------AtVIP1 635 ----------------------------------------------------------------------CeVIP1 661 ----------------------------------------------------------------------CrVIP1 765 MSSVEAAATASVPPSVSPAAAN-----------PTVLPVLQRMIERLAGLKAAARERMAEQQKEAERERECsVIP1 710 AQS-------------------------------------------------------------------DmVIP1 681 ----------------------------------------------------------------------HsVIP1 665 ----------------------------------------------------------------------MpVIP1 664 ----------------------------------------------------------------------OlVIP1 671 ----------------------------------------------------------------------OsVIP1 627 ----------------------------------------------------------------------OtVIP1 692 ----------------------------------------------------------------------PtVIP1 839 ----------------------------------------------------------------------ScVIP1 755 ----------------------------------------------------------------------SPVIP1 603 ----------------------------------------------------------------------TpVIP1 604 ----------------------------------------------------------------------UmVIP1 582 ----------------------------------------------------------------------VCVIP1 761 GGGGGGGGGQGLLQGYSSGDASSVGGGGTATPPPYVLPVLQRMVDRLQALKAAARQREEELLKEQKRREEAtVIP2 625 ----------------------------------------------------------------------CrVIP2 1156 SPLRPTPP--------------------------------------------------------------HsVIP2 653 ----------------------------------------------------------------------OsVIP2 625 ----------------------------------------------------------------------VcVIP2 1134 NMLRTTGPPPPPAPPSGGCGGP------------------------------------------------

AnVIP1 750 ----------------------------------------------------------------------AtVIP1 635 ----------------------------------------------------------------------CeVIP1 661 ----------------------------------------------------------------------CrVIP1 824 REAVVATGAAAAAEKEQKEKEERAANPGPRPASSGGAAEPATEGAATAGADEADDEGPAGGEPEAKLPAACsVIP1 713 ----------------------------------------------------------------------DmVIP1 681 ----------------------------------------------------------------------HsVIP1 665 ----------------------------------------------------------------------MpVIP1 664 ----------------------------------------------------------------------OlVIP1 671 ----------------------------------------------------------------------OsVIP1 627 ----------------------------------------------------------------------OtVIP1 692 ----------------------------------------------------------------------PtVIP1 839 ----------------------------------------------------------------------ScVIP1 755 ----------------------------------------------------------------------SPVIP1 603 ----------------------------------------------------------------------TpVIP1 604 ----------------------------------------------------------------------UmVIP1 582 ----------------------------------------------------------------------VCVIP1 831 EAAAAAAAAAAAAQGAGGEVEKAGQKEGQD--SNVGAVEDGCDGGVEVEVDAAQ-QPDSAEETAAAAAAVAtVIP2 625 ----------------------------------------------------------------------CrVIP2 1164 ----------------------------------------------AASPTATAAAPATPLPPSQSPPPSHsVIP2 653 ----------------------------------------------------------------------OsVIP2 625 ----------------------------------------------------------------------VcVIP2 1156 -------------------------------------ARFPALLPQAAMDVVTMSQMSAGPPPSQPPPPP

AnVIP1 750 ----------------------------------------------------------------------AtVIP1 635 ----------------------------------------------------------------------CeVIP1 661 ----------------------------------------------------------------------CrVIP1 894 PVPAAAAPADAAAVDSAHPAHDHPASGKPPPHPSGAGGLARSSVAAHGAHRPSPLSKRSFEP---AAAAACsVIP1 713 -----------------------------------------------RPLSPMPSGLTSRRASHDGSVSSDmVIP1 681 ----------------------------------------------------------------------HsVIP1 665 ----------------------------------------------------------------------MpVIP1 664 ----------------------------------------------------------------------OlVIP1 671 ----------------------------------------------------------------------OsVIP1 627 ----------------------------------------------------------------------OtVIP1 692 ----------------------------------------------------------------------PtVIP1 839 ----------------------------------------------------------------------ScVIP1 755 ----------------------------------------------------------------------SPVIP1 603 ----------------------------------------------------------------------TpVIP1 604 ----------------------------------------------------------------------UmVIP1 582 ----------------------------------------------------------------------VCVIP1 898 AGEVAAAPAGGQSDAPAPPPPPAQPPLPPPLLPSHPSPAAFGSVTRMEPHRPSPLSKRSLDPSTAAATASAtVIP2 625 ----------------------------------------------------------------------CrVIP2 1188 R------------------------------------------------------------PAAAVAAADHsVIP2 653 ----------------------------------------------------------------------OsVIP2 625 ----------------------------------------------------------------------VcVIP2 1189 QPVPPTKQLQQQQQPPPQHRHQQQQQAEPLLTQRQPSMSCQSSLPPSRTLSTTSSSSSSVAAARRHTAGP

8

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 750 -----------------------IPEPSVVLATVVELMKFHRDIMRHNYEKLDRSYTLAPD---------AtVIP1 635 --------------------AGLPPNAHELLRELVKLTKNVTEQVRLLAMDEDENLTEP-----------CeVIP1 661 --------------------MEFIKNPRKMCHEIAGYVEKMCGVIVEYS---------------------CrVIP1 961 GSAHTHTLLRLGSPSAVAIIAGLPDTPLGLLRRLVELLRKLEDRLRQLVREDGVAGV-------------CsVIP1 736 GAGGGGSRRATEDAAARPVIHPMPQKPLSLLRQLTGHIKVLVEQLRQMCLDERVGRN-------------DmVIP1 681 --------------------LDFVKNPVDCCHHVHLLIRELLHIISIKK---------------------HsVIP1 665 --------------------MTIIQNPVKVCDQVFALIENLTHQIRERM---------------------MpVIP1 664 ------DTATYDDFELSRRPPGVPEEPLKLLRLMIDLIQGLTRQLREKCFRTNVPTH--------MGSPTOlVIP1 671 -------------------LMGVPKQPLKKLEMLYELTKSISVQLRTMLSSHQVRYD--------SDSGTOsVIP1 627 --------------------AGVPPNAANLLTNLAQLTKEITAQVKLLSDNEDEEAVTDS----------OtVIP1 692 --GSPPDTNRGSGFVIKEKLMGVPNQPLRKLEKMYELTKFIAVQLRTTLMAQQTRYD--------MSPGAPtVIP1 839 ------------ALTSLRNALENVGNPRKTLFAIHQTMGELLEQLEEMLGDMGSGDE-------------ScVIP1 755 -----------------------MPEPYLVIKRVVELMNYHKKIMDNNFAKK------------------SPVIP1 603 -----------------------MPKPCEVMQQVVQLMKYHRAVMRENFIILG-----------------TpVIP1 604 ------------RLISLHRALKEIGNPRKTLIAIHSTIGKLVEQLDDMLGELASGDE-------------UmVIP1 582 -----------------------LPPPAKLALEIAGLLAKLRQTMRANYKTLAKG---------------VCVIP1 968 AAAALFSDTLLRATSASSIIARLPDTPLGLLRRLVELLRRLIDRLRQLVREEGATAR-------------AtVIP2 625 --------------------AGLPPHADEHLPELVKLAKKVTEQVRLLAQDEHENLAEPSA---------CrVIP2 1198 AAAVVQNTAAATAADTLSTVRPLPDRPLELLQLLRAQLQDLVEAMRGGGGGAAGSSNPGGSSRSSSGSGNHsVIP2 653 --------------------MHLIKNPVKTCDKVYSLIQSLTSQIRHRM---------------------OsVIP2 625 --------------------AGLPANASQLLPKMAKLTKEVTAQVKLLAEDEDEKLALTN----------VcVIP2 1259 AAGPLPPPPPPSGGPWSAAVKGLPERPLELLEELCRQLRHLVEEIKGPAAAHHAAAHVGAAAVRGSGTGG

AnVIP1 788 -AAEKSDELTSSRAADTSNENPAFSAIQGRWCTGEDPMLFKERWEKLFAEFCDTEKVDP-----SKLS-EAtVIP1 674 ---YDIIPPYDQAKALGKTNIDSDRIASGLPCGSEGFLLMFARWIKLARDLYNERKDR---FDITQIP-DCeVIP1 690 ---------------------QTRPTG-STLYLQESMDLAQRRWNKELREFRRKNKHGEVEFDISKIP-DCrVIP1 1018 -------RAG-AKYSSLSMDPKERVHEDGQPCGGEKMLLMFDRWHKLLKSFHNEKKDR---FDISKVP-DCsVIP1 793 -------GPEGRNYSALTQSPREWGLESGKPCSGERLLLMFDRWRKLLKAFYSDKKQQ---FDISKVP-DDmVIP1 710 ---------------------DDPKTKDAILYHGETWDLMRCRWEKIEKDFSTKSKL----FDISKIP-DHsVIP1 694 ---------------------QDPRSVDLQLYHSETLELMLQRWSKLERDFRQKSGR----YDISKIP-DMpVIP1 720 TTTWVDTLAATAPRGSMPKGGLAELKDGSVPAGGESFLLMHARWKKLEQDIYHPRKGR---YDISKVP-DOlVIP1 714 RINWADTHSAVAPRGSLPKGGIQQLKTMIVPAGGESFLLMYSRWKKLEQDLYHSRKAR---FDISKVP-DOsVIP1 667 ---DSPSHPYDQAKALGKTAIDMDRIASGLPCGSESFLLMFARWKKLERDLYNERKN-------------OtVIP1 752 GITKPDTRSALAPRGSLPKGGIQQLKEMTLPAGGE----------SWQRICTTHGKSD---LIFPRFR-DPtVIP1 884 -------KRIEGGEGIKGENDDDVALSGVKLYKGETLLELTERWRFIYDRLYDSDTDQ---FDLSRIP-DScVIP1 784 ----------------------DVNSMQTRWCTSEDPSLFKERWDKLFKEFNNAEKVDP-----SKIS-ESPVIP1 633 ---------------------PEVEQVQSRWCCNENPALFRERWEKLFSEFCDSEKADP-----SKVS-ETpVIP1 649 -------EVIEGGAGLKGKEENDEALSGIKLYKGETLLELTERWKLLQNKLYDEEKDV---FDLSRVPDAUmVIP1 614 -----------------------IDRVQTRWCTHETPQLFRERWEKLFNDFEE-DPHDP-----SRSS-EVCVIP1 1025 -------GGGPPKYSSLSMDPKERVHDDNQPCGGEKMLLMFDRWHKLLKSFYNEKKDR---FDISKVP-DAtVIP2 666 ---YDVVPPYDQAKALGKSNIDVGRIAAGLPCGSEGFLLMFARWRKLERDLYNERRER---FDITQIP-DCrVIP2 1268 GSSSGRGISSGCYYSSLSMSPDEWRAVPGVPCGGEHWMLVYDRWHKLLDSFYSAKKKQ---YDISKIP-DHsVIP2 682 ---------------------EDPKSSDIQLYHSETLELMLRRWSKLEKDFKTKNGR----YDISKIP-DOsVIP2 665 -----SFSRYDQAKAFGKTTIDVARIAAGLPCGSESFLLMFARWKKLERDLYNERKDR---FDITQIP-DVcVIP2 1329 TGPAEAAPPPHLYYSSLSMSPDEWRSEQGEPCGGERWLLLYDRWHKLLDSFYSPKKGL---YDISKVP-D

AnVIP1 851 LYDSMKFDALHNRQFLEWVFTPPDAEDSDEETADMYRRGSSRNHASAAESRGNGFDGSGHQRVDEQYSESAtVIP1 737 VYDSCKYDLLHNSHLDLKG---------------------------------------------------CeVIP1 737 IYDNIKYDMEHNPDLCINNEVE------------------------------------------------CrVIP1 1076 IYDSAKYDAIHNGHLGL-----------------------------------------------------CsVIP1 852 IYDSAKYDAIHNSELG------------------------------------------------------DmVIP1 754 IYDCIKYDLQHNQH--TLQYDQ------------------------------------------------HsVIP1 738 IYDCVKYDVQHNG---SLGLQG------------------------------------------------MpVIP1 786 VYDAAKYDAIHNSHLLLDG---------------------------------------------------OlVIP1 780 VYDAVKYDAIHNEHLALEG---------------------------------------------------OsVIP1 721 --ILDRYDLLHNSHLKLNG---------------------------------------------------OtVIP1 808 VYDAAKYDAIHNEHLALDG---------------------------------------------------PtVIP1 943 VHDNVRFDVLHNPHLGLTNT--------------------------------------------------ScVIP1 826 LYDTMKYDALHNRQFLENIFDPGLPNEAIADELGSHSLVDRYPINVLAKNNFKIIDSHSMNNSGKNSSNSSPVIP1 676 LYDTLKYDALHNRQFLERIFTP----YQYLKLPQSPSLIAKEPPQRTDSNGNLVGMTGANTN--------TpVIP1 709 VHDNVRFDMLHNPHLGLTET--------------------------------------------------UmVIP1 654 LYDMLSHDGLHNRQFIESVFADPAVTAADVDQR-------------------------------------VCVIP1 1084 IYDSAKYDAIHNAHLGL-----------------------------------------------------AtVIP2 729 VYDSCKYDLLHNSHLDLKG---------------------------------------------------CrVIP2 1334 IYDAAKHALIHNAFIPLTPPPPPPTPPPPSASAATQPPPPPPAQQPPPPPPPQQSHHQQRQAPQQPQPQPHsVIP2 726 IYDCIKYDVQHNG---SLKLEN------------------------------------------------OsVIP2 726 VYDSCKYDLLHNAHLNLEG---------------------------------------------------VcVIP2 1395 IYDAAKHALIHNAYLPLDPPHPPPTPPLAAPAASAAVVAAPGPLRAPSLDSLHLEPLSPGGKPPPPPPPP

9

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 921 QTLAHRLGLKRRMHAFETSIPHLRALDDSYDHYFKLYPSSNPSKTKLDSRLSKLRELYKLAKVLFDYVTPAtVIP1 756 -----------------------------------------------------LDELFKVAQLLADGVIPCeVIP1 759 -----------------------------------------------------FERMYVCVKNMADIVVPCrVIP1 1093 ---------------------------------------------------DVLEELYVVAKLLADVVIPCsVIP1 868 ---------------------------------------------------LTLKPLYTVAKQLADCVIPDmVIP1 774 -----------------------------------------------------AEELYIYAKNLADIVIPHsVIP1 757 -----------------------------------------------------TAELLRLSKALADVVIPMpVIP1 805 -----------------------------------------------------LEELYRISKRLAEGVVPOlVIP1 799 -----------------------------------------------------LEALYEIAKELADCIVPOsVIP1 738 -----------------------------------------------------LSDLFRVSQSLADGVIPOtVIP1 827 -----------------------------------------------------LEELYEIAKELADCIVPPtVIP1 963 -----------------------------------------------------LHKLYECAKYMADCVVPScVIP1 896 VGSLGWV---------------------LESGKTSTARNPKSSSQFDEPRFMQLRELYKLAKVLFDFICPSPVIP1 734 ---------------------------------------------HTERPLEKLYELYDLAKVLFDFVSPTpVIP1 729 -----------------------------------------------------LQKLYDLAKSMADCVVPUmVIP1 687 --------------------------------------------------LVDLHELYRKALALFSFICPVCVIP1 1101 ---------------------------------------------------DALEELYIVAKLLADVVIPAtVIP2 748 -----------------------------------------------------LDELFKVAQLLADGVIPCrVIP2 1404 QPQPQPQPQPQPQPQQPQQPQQQPRSPP--------------DGVVPLLLGEVVAPLYLTARALADAVVPHsVIP2 745 -----------------------------------------------------TMELYRLSKALADIVIPOsVIP2 745 -----------------------------------------------------LEELFKVAQLLADGVIPVcVIP2 1465 QGTARAAAAAEPASPVSSCKHVKRRDTEGTAVSMECTAAPEAGVAPVRVLGEIMAPLYLIARRLADAVVP

AnVIP1 991 QEYGITDTEKLEIGLLTSLPLLQEIVRDLEEVQASSDAKSV-----------------------------AtVIP1 773 NEYGINPQQKLKIGSKIARRLMGKILIDLRNTREEALSVA------------------------------CeVIP1 776 QEYGIKTENKMVIAQRVCTPLLRKIRNDLHRCLENKESEE------------------------------CrVIP1 1112 CEYGLDSGGKLRIGSKIANELLGKLLVDLASMREESMATAFMEPG-------------------------CsVIP1 887 NEYGIDPAGKMRIGSKICCALLGKLLADLANMREESIATAGMEG--------------------------DmVIP1 791 QEYGLTPQEKLAIGQGICSPLLRKIKGDLQRN-IDEVEDE------------------------------HsVIP1 774 QEYGISREEKLEIAVGFCLPLLRKILLDLQRTHEDESVNK------------------------------MpVIP1 822 NEYGTHAHSKLRIGGTIAHSLLVKLLQDMFTTRQESFNIG------------------------------OlVIP1 816 NEYGTTASSKLRIGGTVANSLIAKLLSDLNNTREESFAVE------------------------------OsVIP1 755 NEYGINAKQKLKIGSKIARRLLGKILIDLHNTRREVAAAG------------------------------OtVIP1 844 NEYGTTTSSKLRIGGTIAAGLIGKLLSDLNSTREESFAVE------------------------------PtVIP1 980 QEYGTTVEEKRSVGVKICKGLLEKIMYDLS----------------------------------------ScVIP1 945 KEYGISDAEKLDIGLLTSLPLAKQILNDIGDMKNRETPACV-----------------------------SPVIP1 759 QEYGIEPKEKLEIGLLTSVPLLRQIIHDIKEARDSDHASTR-----------------------------TpVIP1 746 QEYGITVDEKRSIGSKMCATLLEKIKCLFSDILVLLLHLYFTMQI-------------------------UmVIP1 707 REYGITDAEKEEIGFLTSMPLLNNIVQDLKGAKES-NGICS-----------------------------VCVIP1 1120 CEYGLDPGGKLRIGSKIANELLGKLLVDLASMREESIVTACMEP--------------------------AtVIP2 765 NEYGINPQQKLKIGSKIARRLLGKILIDLRNTREEAMSVA------------------------------CrVIP2 1460 HEYGLTPQAQLHVGSAICRELLGKLLCDMGAMREESLATSYSHTGGGGCSSSG-------YNSYSSYSSYHsVIP2 762 QEYGITKAEKLEIAKGYCTPLVRKIRSDLQRTQDDDTVNK------------------------------OsVIP2 762 NEYGINPKQKLKIGSKIARRLLGKILIDLRNTREEAISVS------------------------------VcVIP2 1535 NEYGIAPQSKLVIGSAICRELLGKLLADMGAMREESLATQGGPTSGSSATSSFRSLSGYTLISTTSGGSG

AnVIP1 1032 ----------------------------------------------------------------------AtVIP1 813 --------------------------ELKESQ-EQVLSLSASQREDRNSQPKLFINSDELRRPGTGD-KDCeVIP1 816 ----------------------------------------------------------------------CrVIP1 1157 --AGARGGAGTPGGTLGFNYDQLEGDVGRISLQDEAMVKHHSHGGLRAGPASAVSSSRGEQRDRSGPAPSCsVIP1 931 -----TGEPVSPFDYVDLNPAASAADIPGPAAQGMQQRADAFSATSAPARLDGADTMLSDDSLLNNAASTDmVIP1 830 ----------------------------------------------------------------------HsVIP1 814 ----------------------------------------------------------------------MpVIP1 862 ------------------KPESYNNSGGKTTDGETTDAEKGESSLRRDPSGDPSNGDTLDNEEEEEDEDEOlVIP1 856 ------------------SGGMSSNEMRRVSISEKIPVLNEEDEAE---------------TIDEETEREOsVIP1 795 --------------------------GESNACHDPTIVPSSKRK-DRGYYGDVKNEGFDRPNSNKKSIDLOtVIP1 884 ------------------TGIDSGMRRMSISEKSSAVVLKEEEDGE---------------PIDEEAD--PtVIP1 1010 -----------------------------------VARTDNEVDMR------------------------ScVIP1 986 ----------------------------------------------------------------------SPVIP1 800 ----------------------------------------------------------------------TpVIP1 791 -----------------------------NSFDLAIARTDNQVDMR------------------------UmVIP1 747 ----------------------------------------------------------------------VCVIP1 1164 --HGARGVGGAPG--CGVNYDQLEGEMGRVSLQDEAMVKHGSGG------------------------SNAtVIP2 805 --------------------------ELKNSQDQVSVSLYSSRKEDRYSQPKLFVKSDELRRPSTGENKECrVIP2 1523 SAGGSSGAAAPAAAQRALAAAAAPAAPPRRSHPGFIAQPPAAHTPQPPQPPQPPQQPQQLPHLRSPFSSAHsVIP2 802 ----------------------------------------------------------------------OsVIP2 802 --------------------------DPKFTEDEATFLPTKESEYQQKLQTRNEDGRRSSSTSEKSLDQEVcVIP2 1605 SGGGGGGGAGISRGSGPLGDGEGKDEGERTTRSALGSLVSTVGDNLQLQLSLGHEAAADGVGVGVGVGGG

10

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 1032 ----------------------------------------------------------------------AtVIP1 855 EDDDKE----------------------------------------------------------------CeVIP1 816 ----------------------------------------------------------------------CrVIP1 1225 TSGTGGGGTEGGAAA---DGAPGSAGNTTSGG--------------------------------------CsVIP1 996 SSTGVLGTEETAGNDDEVE---------------------------------------------------DmVIP1 830 ----------------------------------------------------------------------HsVIP1 814 ----------------------------------------------------------------------MpVIP1 914 KAIKEEEETE------------------------------------------------------------OlVIP1 893 REEQEEEEEE------------------------------------------------------------OsVIP1 838 DDSHKE----------------------------------------------------------------OtVIP1 919 --ENNEEIEE------------------------------------------------------------PtVIP1 1021 ----------------------------------------------------------------------ScVIP1 986 ----------------------------------------------------------------------SPVIP1 800 ----------------------------------------------------------------------TpVIP1 808 ----------------------------------------------------------------------UmVIP1 747 ----------------------------------------------------------------------VCVIP1 1206 GPTGREGDLEGGPAG---EMEP------------------------------------------------AtVIP2 849 EDDDKE----------------------------------------------------------------CrVIP2 1593 ASIGPAPPALAAATATPLPGRAGAATAIPAPG----TAAAAGAALVLLPPPPPPHSMVSEFGNASLEDEMHsVIP2 802 ----------------------------------------------------------------------OsVIP2 846 DEDDRE----------------------------------------------------------------VcVIP2 1675 VSGETRGLGLASKAAPVLAPSALVAAAVMSPPGVLERAVTCNDYGGMGGAPQLLDSMVSEFGSASLEDER

AnVIP1 1032 ----------------------------------------------------------------------AtVIP1 861 ----------------------------------------------------------------------CeVIP1 816 ----------------------------------------------------------------------CrVIP1 1254 ----------------------------------------------------------------------CsVIP1 1015 ----------------------------------------------------------------------DmVIP1 830 ----------------------------------------------------------------------HsVIP1 814 ----------------------------------------------------------------------MpVIP1 924 ----------------------------------------------------------------------OlVIP1 903 ----------------------------------------------------------------------OsVIP1 844 ----------------------------------------------------------------------OtVIP1 927 ----------------------------------------------------------------------PtVIP1 1021 ----------------------------------------------------------------------ScVIP1 986 ----------------------------------------------------------------------SPVIP1 800 ----------------------------------------------------------------------TpVIP1 808 ----------------------------------------------------------------------UmVIP1 747 ----------------------------------------------------------------------VCVIP1 1225 ----------------------------------------------------------------------AtVIP2 855 ----------------------------------------------------------------------CrVIP2 1659 LLPTGMAAARQAAVAAAPSPPASPARSAAAGVGSSSRRGGATAGGPAVAPAAAGARAGGAGAGSSTVSPPHsVIP2 802 ----------------------------------------------------------------------OsVIP2 852 ----------------------------------------------------------------------VcVIP2 1745 LLPMSGKATQQQQQQLLPPPPPPPSPKWQQLQEYADTVDVERGTTDESIEAPCETANTVFAVASSDSAEP

AnVIP1 1032 ----------------------------------------------------------------------AtVIP1 861 ----------------------------------------------------------------------CeVIP1 816 ----------------------------------------------------------------------CrVIP1 1254 ----------------------------------------------------------------------CsVIP1 1015 ----------------------------------------------------------------------DmVIP1 830 ----------------------------------------------------------------------HsVIP1 814 ----------------------------------------------------------------------MpVIP1 924 ----------------------------------------------------------------------OlVIP1 903 ----------------------------------------------------------------------OsVIP1 844 ----------------------------------------------------------------------OtVIP1 927 ----------------------------------------------------------------------PtVIP1 1021 ----------------------------------------------------------------------ScVIP1 986 ----------------------------------------------------------------------SPVIP1 800 ----------------------------------------------------------------------TpVIP1 808 ----------------------------------------------------------------------UmVIP1 747 ----------------------------------------------------------------------VCVIP1 1225 ----------------------------------------------------------------------AtVIP2 855 ----------------------------------------------------------------------CrVIP2 1729 LYTVMEECAAHEQQADDREEEGAGGEQAAAAAVEGDGSPPLLHPLGVAGFVGAGEEREQEGEARAAGAAGHsVIP2 802 ----------------------------------------------------------------------OsVIP2 852 ----------------------------------------------------------------------VcVIP2 1815 VAGQPAPPPPPPQRPLTECGMASHRDAAGDGSNAAAFGTPAAAAPAPATDACTKGCRGPDDEMVVAAAVG

11

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 1032 ------------------------------------------------------------------FYFTAtVIP1 861 -------------------------------------------TKYRLDPKYAN-VKTPERHVRTRLYFTCeVIP1 816 -------------------------------------------TQTRLDPRASQGIATPFRHVRTRLYFTCrVIP1 1254 ------------------------------GAGGGEMEPAETETIHRLCPTYASDINSPLRHVRTRIYFTCsVIP1 1015 ---------------------------------------EDRETLHRLCPTYAQDINSPLRHVRTRIYFTDmVIP1 830 -------------------------------------------FMNRLNPHYSHGVASPQRHVRTRLYFTHsVIP1 814 -------------------------------------------LHPLCYLRYSRGVLSPGRHVRTRLYFTMpVIP1 924 ----------------------------------------LSTTRLNHRYANTVGVHSPHRHVRTRLYFTOlVIP1 903 ----------------------------------------LNTTRLNLRYATAHGVHSPFRHVRTRLYFTOsVIP1 844 -------------------------------------------TKYCLDPKYAN-VMEPERRVRTRLYFTOtVIP1 927 ----------------------------------------LNTTRLNLRYATAHGVHSPFRHVRTRLYFTPtVIP1 1021 -------------------------------------------YMINMDYSADLPINTMGRRIRTRLYFTScVIP1 986 ------------------------------------------------------------------AYFTSPVIP1 800 ------------------------------------------------------------------MYFTTpVIP1 808 -------------------------------------------YLINMDYSADLPINSMGRRVRSRLYFTUmVIP1 747 ------------------------------------------------------------------LYFTVCVIP1 1225 ----------------------------------------ETETIHRLCPTYASDINSPLRHVRTRIYFTAtVIP2 855 -------------------------------------------TKYRLDPKYAN-VMTPERHVRTRLYFTCrVIP2 1799 AAGGSCGGGGPPEQQQQEGEEQVEGEGDDDGLVGGPPPPPPLEALHRLCPQYATDINSPLRHVRTRVYFTHsVIP2 802 -------------------------------------------LHP----VYSRGVLSPERHVRTRLYFTOsVIP2 852 -------------------------------------------TKYRLDPKYAN-VKTPDRHVRTRLYFTVcVIP2 1885 EGGGGDGGGLP------------EGEDDSNNGIVGDGVEEEAQALHRLCPQYATDINSPLRHVRTRVYFT

AnVIP1 1036 KESHIYTLLNCILEGGVQTKIA------------------------------------------------AtVIP1 887 SESHIHSLMNVLRYCNLDESLL------------------------------------------------CeVIP1 843 SESHIHTLMNLIRYGNLCS-VDDKK---------------------------------------------CrVIP1 1294 SESHMHSLVNVLRWCHEGPEAADCCPPP------ITPPSGTGGNSSPDLRASAGGAAAAAVAAALATEGGCsVIP1 1046 SESHMHSLINVLRFAHLS----------------------------------------------------DmVIP1 857 SESHVHSLLTVLRYGGLLNVVTDEQ---------------------------------------------HsVIP1 841 SESHVHSLLSVFRYGGLLDETQDAQ---------------------------------------------MpVIP1 954 SESHIHSLLNVLKYCNLEAFTK------------------------------------------------OlVIP1 933 SESHLHSLINVLQYAHLDKPRE------------------------------------------------OsVIP1 870 SESHIHSLMNVLRYCNFDESMD------------------------------------------------OtVIP1 957 SESHLHSLLNVLQYAHLDNTRD------------------------------------------------PtVIP1 1048 SESHLHTVLNALRFAGDG----------------------------------------------------ScVIP1 990 KESHIYTLLNIIYESGIPMRIA------------------------------------------------SPVIP1 804 KESHIYTLLNCILESGLPMKLP------------------------------------------------TpVIP1 835 SESHLHSLLNVLRFASAQPE--------------------------------------------------UmVIP1 751 KESHIHTLLNLVLAS--ELNVV------------------------------------------------VCVIP1 1255 SESHMHSLVNVLRWCHLGADAAGCCAMPP-----ITPPSGTGGTSSPDLRASAGGAPTAAAAAAAMAAAAAtVIP2 881 SESHIHSLMNVLRYCNLDESLQ------------------------------------------------CrVIP2 1869 SESHIHALLNVLRYCHLRQQQQ------------------QQQQQPPAPTAATAATAAAQPSSPAASATSHsVIP2 825 SESHVHSLLSILRYGALCNESKDEQ---------------------------------------------OsVIP2 878 SESHIHSLMNVLRYCNLDECLQ------------------------------------------------VcVIP2 1943 SESHIHALLNVLQYCHLHQQQQPTSNTVSTAATTNVCTAWQPGRGNDAAAAAPLLASSSSEAAVPAGHHP

AnVIP1 1058 ------------------------------------------------------------RSAIPELDYLAtVIP1 909 -----------------------------------------------GEESLICQNALERLCKTKELDYMCeVIP1 867 -----------------------------------------------------WQRAMNFLSGVTEFNYMCrVIP1 1358 -------------------------ASSPVAGAASGAAGEPAYGRQFDNSPLLSANACAQLDDTTELDYLCsVIP1 1064 --------------------------------------------RDIPTESLVSDAAQEMLRNTTELDYMDmVIP1 882 -----------------------------------------------------WRRAMDYISMVSELNYMHsVIP1 866 -----------------------------------------------------WQRALDYLSAISELNYMMpVIP1 976 -----------------------------------------------TNPPSLVSHRLETLGGIGDLDYLOlVIP1 955 -----------------------------------------------DRERGRSPYHTEHSE-SDELDYLOsVIP1 892 -----------------------------------------------GEESLVCKNALDNLFKTRELDYMOtVIP1 979 -----------------------------------------------VEREGRSAQSAAASD-QSDGDKPPtVIP1 1066 -----------------------------------------------GANPLLSEHGVSIINGTPELCYLScVIP1 1012 ------------------------------------------------------------RNALPELDYLSPVIP1 826 ------------------------------------------------------------RNQIPELDYLTpVIP1 855 -----------------------------------------------SVTSPLSVRGQEILASASELCYLUmVIP1 771 ------------------------------------------------------------MPKTPPLDYFVCVIP1 1320 NAFARGGGGGGSGGLDSANSPLMAAAGMAATAAAGGNVGGASFGRQYDSSPLLSEGACAQLDEMTELDYLAtVIP2 903 -----------------------------------------------GEESLVCQSALDRLCKTKELDYMCrVIP2 1921 LGPSGGSSTSAAGVAGSTAAPAAAAATSVPADDLSPAQPQPQPQPPEQPATLLGAAGEALLAGVPEYDYMHsVIP2 850 -----------------------------------------------------WKRAMDYLNVVNELNYMOsVIP2 900 -----------------------------------------------GEDSLVCQSALDRLHRTRELDYMVcVIP2 2013 LPPAGDLGLGGTHTTEGVAAPEGAPVAPHHYHQQQQQQQQQQHGKQVLSATLLGSPGQELLEAAPEYDYM

12

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 1068 SQICFELYEA-----------RDSESSTSS------------YSIRISISPGCHAFDPLDVHLDSRHAIGAtVIP1 932 SYIVLRLFEN----------TEVSLEDPK------------RFRIELTFSRGADLSPLRNND--------CeVIP1 884 TQVVLMVYEDSRKEN-------DEADTGP------------RFHIEILFSPGLYPCFLTEKERIYETRFNCrVIP1 1403 TQVVFRMYEN----------KTVPVESPE------------RFRVEVLFSPGANYNPFDFTMP-LHNNHVCsVIP1 1090 THIVFRMYEN----------KRVPVESAE------------RFRVEVLFSRGAGHN--PFEVIPIKQDHTDmVIP1 899 SQIVIMLYEDPTKD----------PTSEE------------RFHVELHFSPGVN-CCVQKNLPPGPGFRPHsVIP1 883 TQIVIMLYEDNTQD----------PLSEE------------RFHVELHFSPGVKGVEEEGSAPAGCGFRPMpVIP1 999 THIVFRMYEC----------FDVPAEDPN------------RFRIEILLSTGIGLDPFKQN---------OlVIP1 977 THIVFRMFER----------FHVPPSDPR------------RFRIEILFSNGVSLHPFKTNAIELFDSSAOsVIP1 915 SYIVLRMFEN----------TEVSLEDPK------------RFRIEMTYSRGADISSLQFLKNVSIQSEHOtVIP1 1001 SALIFTPGP------------RSSPSKPP------------GLPLGSPPMRSPTLGSPKGEKSVQRGAQPPtVIP1 1089 TQIVMRVFED----------SRREMHDPR------------RFRVEILFSPGATATPFHLHEGDREQDTSScVIP1 1022 SQITFELYES-----------TDA-SGQKS------------HSIRLKMSPGCHTQDPLDVQLDDRHYISSPVIP1 836 TQICFELFER-----------TNP-SGNKE------------FSVRITLSPGCYAQCPLDMNLDAKHCISTpVIP1 878 TQVVIRLFED----------TQKPAEDPR------------RFRVEIWFSPGATATPL------------UmVIP1 781 ASITFEVYEN-----------TSTSTLDNSGSSTSLSAPSQQYSLLISVSEGAHSSNILSINLDARHALTVCVIP1 1390 TQVVFRMYEN----------KTVPVESQE------------RFRVEVLFSPGANYNPFDFASTSLHNNHVAtVIP2 926 SYVVLRLFENTETLKHKNYCLQISLDDPK------------RFRIELTFSRGADLSPLEKKD--------CrVIP2 1991 TQIVFRMFEN----------RHVSAAAGGQEEAGGGGGSGCRFRVEVLFSPGASRAALEARAQSMLARGRHsVIP2 867 TQIVIMLYEDPNKD----------LSSEE------------RFHVELHFSPGAKGCEEDKNLPSGYGYRPOsVIP2 923 SNIVLRMFEN----------TEVPLEDEK------------RFRIEMTFSRGADLSPLEDKT--------VcVIP2 2083 TQIVFRMFEN----------KHVSYLEVI-----------------------------------------

AnVIP1 1115 CAPRRSLTAHQDWKEV------------------------------------------------------AtVIP1 972 DEAETLLREHTLPIMG------------------------------------------------------CeVIP1 935 LSTNPKPATSSRSSGRESRDTNDSASSSTEGRRPSIEKVVTVVTPTQLSTPSVTNDDLSISSNAESTAAECrVIP1 1450 LPTIPRTALHKGDG--------------------------------------------------------CsVIP1 1136 LPIALRTPLHRDTG--------------------------------------------------------DmVIP1 946 HSHG----DNACNVSLQSSDESNPARIEEENDSNSGEEREGKKRGTSGQRSTDRSAERISPAFGFNRLELHsVIP1 931 ASSE----NEEMKTNQGSMENLCPGKASDEPD-RALQTSPQPPEGPGLPRRSPLIRNRKAGSMEVLSETSMpVIP1 ----------------------------------------------------------------------OlVIP1 1025 RNGVADEDEEMISDD-------------------------------------------------------OsVIP1 963 GKDSLLPDDHTMKIME------------------------------------------------------OtVIP1 1047 DEAQPQVRASLINDS-------------------------------------------------------PtVIP1 1137 RFDTAPLKMIGREGLT------------------------------------------------------ScVIP1 1068 CIPKISLTKHLDMDYV------------------------------------------------------SPVIP1 882 VSPRRSLTRHLDLQQF------------------------------------------------------TpVIP1 ----------------------------------------------------------------------UmVIP1 840 PLPRRPLTTHMDFDDA------------------------------------------------------VCVIP1 1438 LPIVPRTALHKNDG--------------------------------------------------------AtVIP2 976 EEAESLLREHTLPIMG------------------------------------------------------CrVIP2 2051 GRGRRRGRRRRQGAGMARGEEAAGGEEGERQRQQRLGGGCSSSDSSSGEGEEEEEEEGGREGGVGVGGGGHsVIP2 915 ASRE----NEGRRPFKIDNDDEPHTSKRDEVDRAVILFKPMVSEPIHIHRKSPLPRSRKTATNDEESPLSOsVIP2 963 SENTSLLQEHTLPIMG------------------------------------------------------VcVIP2 ----------------------------------------------------------------------

AnVIP1 1131 -----------------------------IETLKAKFNTFYHGFMTKFDKRMSATNNLQNYR--------AtVIP1 988 -----------------------------PERLQEVGSCLSLETMEKMVRPFAMPAEDFPPA--------CeVIP1 1005 STGLVNTTTKTHNDSEDDLNDVESVNLVALDELNNTTKAMADDGKTAKRQRSVTGAEKSMEEGDKPHGEWCrVIP1 1464 ---------------------------ITLAEMEEKLLPFSQTRKFNTCSYALQYSLRAPHL--------CsVIP1 1150 ---------------------------TTWSQLSELLSPHSTPAKASTYTYSMQ----------------DmVIP1 1012 RSKQFKSKPIPIGAHHTVSGHEAMDLAKRLNEELASHQQQQNQQLRPISPDIRAVTPDCEPRSRSFEQRPHsVIP1 996 SSRLFSTTVLGGSSSAPNLQDYARSHGKKLPPASLKHRDELLFVPAVKRFSVSFAHNALSLRQVSEFLSRMpVIP1 ----------------------------------------------------------------------OlVIP1 1040 --------------------------LLKEIQIQNDKIMPGQDYLTLSTMEEYLVNYNRRWN--------OsVIP1 979 -----------------------------PERLQEVGSYLTLDKFDKMVRPFAMPAEDFPPA--------OtVIP1 1062 --------------------------LRALISREKELDYLTHIVFRMFERFQVPPSDPRRFR--------PtVIP1 1153 ---------------CAEVEEFFEKVIMAGRTEEDENELASTSTATVDAKAPKDKSKKKKTV--------ScVIP1 1084 -----------------------------QQKLRNKFTRVIMPPKFTPVNITSPNLSFQKRK--------SPVIP1 898 -----------------------------ITKTEDLCNSVHLPKRFIPVNIN------------------TpVIP1 ----------------------------------------------------------------------UmVIP1 856 -----------------------------MAKLEAHSSRDSRVDRGMVEGSTVFFGQDDPDR--------VCVIP1 1452 ---------------------------ITLAEMEEKLMPFSSTRKFTKCSYALQYSLRAPHL--------AtVIP2 992 -----------------------------PERLQEVGSCLTLETMEKMIRPFAMPAEDFPPP--------CrVIP2 2121 GGDALEADRHCLPLEPLVHLNAGPGAARALEALEALLAPLAAPRKAQPNTYALQVALKAPGA--------HsVIP2 981 VSSPEGTGTWLHYTSGVGTGRRRRRSGEQITSSPVSPKSLAFTSSIFGSWQQVVSENANYLRTPRTLVEQOsVIP2 979 -----------------------------PERLQEVGSCLTLDKFEKMVRPFAMPPEDFPPA--------VcVIP2 ----------------------------------------------------------------------

13

Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 1164 -------------------------------------------------------------KGRIRIGTMAtVIP1 1021 -------------------------------------------------------STPVGFSGYFSKSAACeVIP1 1075 KGNGVAKSGSQISVGSNEMESNNESMETVGGGKGQWVKDLLDQTKRAMAMNSIREVEPPIVIPTPVPSTTCrVIP1 1499 -------------------------------------------------------IHGGVNSRYGSTGALCsVIP1 ----------------------------------------------------------------------DmVIP1 1082 TSG-------------------------------------VCAKEPDGRVSVSVSASVSSANASTSSRRQHsVIP1 1066 VCQRHTDAQAQASAALFDSMHSSQASDNPFSPPRTLHSPPLQLQQRSEKPPWYSSGPSSTVSSAGPSSPTMpVIP1 ----------------------------------------------------------------------OlVIP1 1076 -------------------------------------------------------KGGDSADGTPGKGAROsVIP1 1012 -------------------------------------------------------APSQSLAVRFCKDNGOtVIP1 1098 -------------------------------------------------------IETCLHGVN-S----PtVIP1 1200 -------------------------------------------------------NLDNGTAREIGKTSAScVIP1 1117 -------------------------------------------------------------TRRKSVSVESPVIP1 ----------------------------------------------------------------------TpVIP1 ----------------------------------------------------------------------UmVIP1 889 -------------------------------------------------------------QYMPIKNRRVCVIP1 1487 -------------------------------------------------------HIG-SRYGSGGNLVQAtVIP2 1025 -------------------------------------------------------CTPAGFSGYFSKSAACrVIP2 2183 -------------------------------------------------------ATADSATATATFGGGHsVIP2 1051 KQN------------------------------------------------------PTVGSHCAGLFSTOsVIP2 1012 -------------------------------------------------------AAPQAFSGYFKG-SGVcVIP2 ----------------------------------------------------------------------

AnVIP1 1173 ----------------------------------------------------------------------AtVIP1 1036 VLERLVNLFHNYKNSSSNGRSD------------------------------------------------CeVIP1 1145 TAVVEDEASERQSRSRRYFPYRFKHHTAQLLTGMSGGGVHMQNRLISTDVLTGKFGDHDNKKNSRKDFGACrVIP1 1514 GHNGRSTASIAGNDSTGDLAATKGAGKEKAVDKEAKEKEAKEKETKEKSAEKEKEAKEKSVEKEKEANEACsVIP1 ----------------------------------------------------------------------DmVIP1 1115 RRGIAGRMSCMKMLGFGGFSKKMATSANSLFSTAVISGSSSAPNLRDMITVSSSGFGDVPPIRPLETLHNHsVIP1 1136 TVDGNSQFGFSDQPSLNSHVAEEHQGLGLLQETPGSGAQELSIEGEQELFEPNQSPQVPPMETSQPYEEVMpVIP1 ----------------------------------------------------------------------OlVIP1 1091 SSSVGSERIRAGKNLFT-----------------------------------------------------OsVIP1 1027 LQGARLVPYPVLYHRMILPFYFRENSIS------------------------------------------OtVIP1 ----------------------------------------------------------------------PtVIP1 1215 EINSFPPSASVEPDTVTLLKSRTEKGLPESKTDVRPGEAESVHSTNEKPADSIAIPADSHLNVPFKDREAScVIP1 1126 KLKRPASSGSSSSTSVNKTLDN------------------------------------------------SPVIP1 ----------------------------------------------------------------------TpVIP1 ----------------------------------------------------------------------UmVIP1 898 DSVDTDQSSVRHA---------------------------------------------------------VCVIP1 1501 PPGARNNVSMPGNDSQTDMASKQAPQAPQAPQASQAPQQQQVAGAGTGFQPGAPSAETQSQP--------AtVIP2 1040 VLERLVKLWPFHKNTSNGKS--------------------------------------------------CrVIP2 2198 QLPAGSSTSGSSTSGSSTSYMRRELAAAVASRAPSTALRGPAPLAPDMLLPALGAMATAVADEAAAATAAHsVIP2 1067 SVLGGSSSAPNLQDYARTHRKKLTSSGCIDDATRGSAVKRFSISFARHPTNGFELYSMVPSICPLETLHNOsVIP2 1026 MLERLASLWPFHKGATNGK---------------------------------------------------VcVIP2 ----------------------------------------------------------------------

AnVIP1 ----------------------------------------------------------------------AtVIP1 ----------------------------------------------------------------------CeVIP1 1215 GTAVLSTAVIARSSSAPRLMTYESEDFSVGEIKRFWPPLRSLETLHDSINLSQFDGFLERLIKGALTPLPCrVIP1 1584 ----------------------------------------------------------------------CsVIP1 ----------------------------------------------------------------------DmVIP1 1185 ALSLRKLDSFLQDMILAQIFKTPTGSPPRGFSKNTLPAVSSMTLTASNQTEVVEHEPISTTVTPTFDRRGHsVIP1 1206 SQPCQEVPDISQPCQDISEALSQPCQKVPDISQQCQENHDNGNHTCQEVPHISQPCQKSSQLCQKVSEEVMpVIP1 ----------------------------------------------------------------------OlVIP1 ----------------------------------------------------------------------OsVIP1 ----------------------------------------------------------------------OtVIP1 ----------------------------------------------------------------------PtVIP1 1285 EASKIVARKYFWSTVAVGSFTLAVGCLYMAFSMGDDTRRRRRWASRRN----------------------ScVIP1 ----------------------------------------------------------------------SPVIP1 ----------------------------------------------------------------------TpVIP1 ----------------------------------------------------------------------UmVIP1 ----------------------------------------------------------------------VCVIP1 ----------------------------------------------------------------------AtVIP2 ----------------------------------------------------------------------CrVIP2 2268 ARAAVLGGSTAAAAVQPRPPAAAAAEGAPGAGSGGDAATANTGARGGNGGAAGTGGLPPGAATARLHPYMHsVIP2 1137 ALSLKQVDEFLASIASPSSDVPRKTAEISSTALRSSPIMRKKVSLNTYTPAKILPTPPATLKSTKASSKPOsVIP2 ----------------------------------------------------------------------VcVIP2 ----------------------------------------------------------------------

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Supplemental Data. Couso et al. (2016). Plant Cell 10.1105/tpc.15.00351AnVIP1 -------------------------------------------------AtVIP1 -------------------------------------------------CeVIP1 1285 SPPKTPLPSALSCDAINKTPTQDEVEKVIGKLAPTSSTD----------CrVIP1 -------------------------------------------------CsVIP1 -------------------------------------------------DmVIP1 1255 SESGSTADAHLRLLSESQCPNLDDSNTELRESLAGKMEQYPTEPSIK--HsVIP1 1276 CQLCLENSEEVSQPCQGVSVEVGKLVHKFHVGVGSLVQETLVEV-----MpVIP1 -------------------------------------------------OlVIP1 -------------------------------------------------OsVIP1 -------------------------------------------------OtVIP1 -------------------------------------------------PtVIP1 -------------------------------------------------ScVIP1 -------------------------------------------------SPVIP1 -------------------------------------------------TpVIP1 -------------------------------------------------UmVIP1 -------------------------------------------------VCVIP1 -------------------------------------------------AtVIP2 -------------------------------------------------CrVIP2 2338 QDGLQSAASLPVLPSHRLRAAAALMAAGGGGAAAAAAAAAADVAAAAKEHsVIP2 1207 ATSGPSSAVVPNTSSRKKNITSKTETHEHKKNTGKKK------------OsVIP2 -------------------------------------------------VcVIP2 -------------------------------------------------

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