Cancer treatment with combined autologous p53 dendritic cell vaccine and adenovirus-p53 gene therapy
· Web viewKAISO induces apoptosis only in wild type p53-expressing in H1299 cells. (A) Flow...
Transcript of · Web viewKAISO induces apoptosis only in wild type p53-expressing in H1299 cells. (A) Flow...
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SUPPLEMENTARY DATA
Temporal and differential regulation of KAISO-controlled transcription by phosphorylated and acetylated p53 highlights a crucial regulatory role of apoptosis
Seo-Hyun Choi, Dong-In Koh, Su-Yeon Cho, Min-Kyeong Kim, Kyung-Sup Kim, and Man-Wook Hur*
Brain Korea 21 Plus Project for Medical Sciences, Department of Biochemistry and Molecular Biology, Severance Biomedical Research Institute, Yonsei University
School of Medicine, 50-1 Yonsei-Ro, SeoDaeMoon-Ku, Seoul 03722, Korea
*To whom correspondence may be addressed: Man-Wook Hur, Department of Biochemistry and Molecular Biology, Yonsei University School of Medicine, 134, ShinChon-Dong, SeoDaeMoon-Ku, Seoul, Korea 120-752, Tel: 82-2-2228-1678, Fax: 82-2-312-5041; E-mail: [email protected]
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SUPPLEMENTARY FIGURES
Supplementary Figure 1. Induction of KAISO expression by other DNA damaging
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reagents is p53-dependent. (A, B) RT-qPCR and western blot analysis of KAISO and p53
expression. HCT116 p53+/+, HCT116 p53-/- cells were treated with cisplatin (20 µM) or 5-FU (50
µM) harvested at the indicated times, and analyzed for mRNA by RT-qPCR, and protein
expression by western blot analysis, respectively. GAPDH, control.
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Supplementary Figure 2. KAISO induces apoptosis only in wild type p53-expressing in
H1299 cells. (A) Flow cytometry analysis. The SNU61, Colo320DM, LS1034, HT-29 cells
expressing endogenous p53 “hot spot” mutant was transfected with KAISO expression or
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control vector and were stained with propidium iodide and annexin V, using and apoptosis
detection kit (BD Bioscience). (B) Flow cytometry analysis of apoptosis of H1299 p53-null
cells transfected with KAISO, WT p53, p53R175H, p53G245S, p53R248W, and p53R273H
expression vector in the combination indicated.