· Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m)...

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Appendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites in physiological saline for 1 hr

Transcript of  · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m)...

Page 1:  · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites

Appendix 1. Steps followed during preparation of permanent slides (Slides a – m)

Slide a. Collected parasites in petridish

Slide b. Relaxation of collected parasites in physiological saline for 1 hr

Page 2:  · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites

Slide c. Fixation of parasites in 10% formalin

Slide d . Washing of parasites in running water for 6 hrs

Page 3:  · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites

Slide e. Staining the parasites with Borax Carmine for overnight

Slide f. De-stain the parasites in acid alcohol until the stain removed

Page 4:  · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites

Slide g. Dehydration in 90% ethanol for 15 minutes

Slide h. Dehydration in 95% ethanol for 15 minutes

Page 5:  · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites

Slide i. Dehydration in absolute alcohol for 30 minutes

Slide j. Dehydration in acid alcohol for 30 minutes

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Slide k. Cleared in Lectophenol for 15 minutes

Slide l. Mounted in Canada Balsam Slide m. Prepared permanent slides


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