Blood Chapter 12 Bio160. Blood Composition Red blood cells White blood cells Platelets Plasma.
W1130 A DIAGNOSTIC TOOL FOR RED BLOOD CELL DISORDERS … · Our newly designed RBC chips for...
Transcript of W1130 A DIAGNOSTIC TOOL FOR RED BLOOD CELL DISORDERS … · Our newly designed RBC chips for...
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Red blood cells (RBCs) are a very important part
of every human being.
Besides carrying oxygen, RBCs are playing a
pivotal role in different disorders ranging from
different types of anemia over inherited
membrane disorders to parasitic infections.
Additionally, neurological disorders can alter
membrane elasticity and thereby impairing
oxygen transport. Thus, RBCs are representing a
very interesting and important cell population as
biomarker carrier in several diseases.
Tools, to evaluate the morphology of RBCs as well
as expression and staining patterns of different
biomarkers is highly anticipated.
Our newly designed RBC chips for Chipcytometry
only need a few drops of blood to analyze these
parameters on a high amount of Red Blood Cells.
In parallel.
The iterative process of staining and bleaching
allows the detection of different markers on the
same cell. In addition, storage of RBCs on the chip
is possible for later analyzing of interesting
markers.
CONCLUSIONS
With analyzing and storage of RBCs on a chip we offer an
outstanding new tool for red blood cell research.
The new modified chip surface allows the recovery and storage
of red blood cells. Besides analyzing different RBC specific
surface markers (CD235a and CD238) , the detection of
erythroid precursors is possible. Additionally, other markers –
not necessarily specific for RBCs- are detected. Storage of RBCs
is possible up to 20 weeks, with only slight decrease of marker
expression.
As image generation and analysis of the results can be
performed automatically on a high number of cells there is
nearly no limit of throughput to reach statistical significance in
a study.
METHODS AND RESULTS
ZellSafe Microfluidic Chips were modified to contain a cell-adhesive surface suitable for RBC recovery. Heparinized blood was diluted and RBCs loaded on the chip. Biconcavity – a hallmark of RBCs – was retained after immobilization in the chip.
Expression of several markers was analyzed on fresh RBCs.
ZELLKRAFTWERK GmbH Deutscher Platz 5c · 04103 Leipzig Germany Phone: +49 (441) 3508 2403 Fax: +49 (511) 532 8725 E-Mail: info[at]zellkraftwerk.com
A DIAGNOSTIC TOOL FOR RED BLOOD CELL DISORDERS –
CHIPCYTOMETRY AS A NEW RBC ANALYSIS PLATFORM
Anke Hofmeister-Brix, Christian Hennig
ZELLKRAFTWERK GmbH, Germany
www.zellkraftwerk.com
Marker stability was analyzed after different time points.
Storage of RBCs does not impact bioconcavity. All markers were still detectable even after 25 weeks of storage.
RBCs stored with addition of PBMCs
RBC-Storage test 1 (without addition of PBMCs) M1030 -04-025
M1030 -04-025
W1130 -04-028